• Enables detection of low abundance targets. (cellsignal.com)
  • Multispectral imaging systems support software enabling linear unmixing of fluorophores (and chromogens) that have overlapping excitation/emission spectra and thus promote multiplex target detection with superior resolution. (cellsignal.com)
  • Detection of gene targets are coded by a library of 64 distinct Masscode tags (Qiagen Masscode technology, Qiagen, Hilden, Pathogens Germany). (cdc.gov)
  • Secondary antibodies facilitate signal detection and amplification. (bdbiosciences.com)
  • In fluorescence-based secondary detection, fluorophore-conjugated secondary antibodies are used to bind the primary antibody and the signal is detected using a digital imager. (bdbiosciences.com)
  • This method either uses autoradiography or a digital imager for signal detection. (bdbiosciences.com)
  • After amplification the samples are typed by hybridization to the typing strips followed by colorimetric detection. (cdc.gov)
  • In the present study, we developed and evaluated rapid, sensitive and robust C. psittaci -specific and C. pecorum -specific Loop Mediated Isothermal Amplification (LAMP) assays for detection of these pathogens. (peerj.com)
  • The digene HC2 High-Risk HPV DNA Test is an in vitro nucleic acid hybridization assay with signal amplification and chemiluminescence for the qualitative detection of 13 types of human papillomavirus (HPV) in cervical specimens. (qiagen.com)
  • The digene HC2 High-Risk HPV DNA Test detects the presence of 13 high-risk HPV types (16/18/31/33/35/39/45/51/52/56/58/59/68, which are carcinogenic) using full genome probes complementary to HPV DNA, specific antibodies, signal amplification, and chemiluminescent detection. (qiagen.com)
  • Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. (thermofisher.com)
  • Rapid, sensitive and precise probe-based qPCR detection and quantitation of target RNA targets. (neb.com)
  • Here we explore the possibility that PCR amplification of transposon insertions in a TnSeq library skews the results by introducing bias into the detection and/or enumeration of insertions. (microbiologyresearch.org)
  • Here, researchers from Southeast University developed a fluorescent biosensing platform, namely a dual signal amplification, for the ultrasensitive detection of leukemia cell-derived exosomes. (exosome-rna.com)
  • A dual signal amplification system with specific signal identification for rapid and sensitive detection of miRNA. (bvsalud.org)
  • False positive which is mostly caused by the nonspecific amplification has severely hindered the development of nucleic acid detection and it is hard to avoid. (bvsalud.org)
  • Herein, we proposed a one-step and rapid miRNA detection strategy with specific signal identification, dual amplification and output. (bvsalud.org)
  • The most dominant techniques are PCR viral detection and signal amplification DNA-based assays (Hybrid Capture II is the only FDA-approved technique at present). (medscape.com)
  • CoV-2 genome and can be targeted as a region for detection of VOC/VOI. (who.int)
  • ABSTRACT We evaluated the utility of 2 methods for detection of methicillin-resistant Staphylococcus aureus (MRSA) directly from signal-positive blood culture bottles: loop-mediated isothermal amplification (LAMP) assay, and direct cefoxitin disk diffusion (DCDD) test using a 30 g cefoxitin disk. (who.int)
  • Nucleic acid amplification tests, in particular, have emerged as a preferred approach for TB detection. (cdc.gov)
  • These steps aim to remove nonspecific hybrids and get rid of unbound probe molecules from the samples to reduce any background signaling. (wikipedia.org)
  • Thus HRP-catalyzed deposition of tyramide-fluorophore molecules provides enhanced amplification of fluorescence signal at the site of the antigen. (cellsignal.com)
  • The expression of EMT-related molecules and Jagged-1/Notch signaling pathway was analyzed by Western blot and qPC experiments. (researchsquare.com)
  • In this review, we discuss the changes in irradiated cancer cells and immune cells in the TME under different RT regimens and describe existing and potential molecules that could be targeted to improve the therapeutic effects of RT. (nature.com)
  • Though this technology can be used to detect a variety of molecules, this dissertation establishes the utility of microring resonator chips for multiparameter analysis of several challenging protein targets in cell cultures, human blood sera, and other clinical samples such as cerebrospinal fluid. (illinois.edu)
  • To do so, cells release signaling molecules that can move around within a tissue and be detected by receptors on other cells. (elifesciences.org)
  • We tend to assume that the signaling molecules are evenly distributed across a tissue and affect all the receiving cells in the same way. (elifesciences.org)
  • First of all, it is well-known that the electrode materials play a critical role in the construction of high-performance electrochemical sensing platforms for detecting target molecules through various analytical principles. (cdc.gov)
  • The expression of anti-inflammatory cell signaling molecules, including c-Jun N-terminal kinase (JNK), p38, extracellular signal regulated kinase (ERK), Akt, nuclear factor-κB (NF-κB), inhibitory-κBα (IκBα), iNOS and COX-2, was evaluated by western blot analysis. (spandidos-publications.com)
  • Given the vast archive of formalin-fixed and paraffin-embedded (FFPE) tissue blocks in transplant centers, a reliable protocol for utilizing this tissue bank for clinical validation of target molecules as predictors of graft outcome over time, would be of great value. (escholarship.org)
  • The LAMP assays, performed in a Genie III real-time fluorometer, targeted a 263 bp region of the C. psittaci -specific Cps_0607 gene or a 209 bp region of a C. pecorum -specific conserved gene CpecG_0573, and were evaluated using a range of samples previously screened using species-specific quantitative PCRs (qPCRs). (peerj.com)
  • Both LAMP assays were demonstrated to species-specific, highly reproducible and to be able to detect as little as 10 genome copy number/reaction, with a mean amplification time of 14 and 24 min for C. psittaci and C. pecorum , respectively. (peerj.com)
  • Fields such as medical diagnostics and cellular biology would benefit greatly from a technology that enables rapid, quantitative and reproducible assays for many targets within a single sample. (illinois.edu)
  • Is amplification bias consequential in transposon sequencing (TnSeq) assays? (microbiologyresearch.org)
  • Methods: We designed and optimized assays to quantify 19 target genes, including previously reported set of tissue common rejection module (tCRM) genes. (escholarship.org)
  • Probe-based assays have the advantages of high specificity and the possibility to detect multiple targets in the same vessel by combining probes labeled with dyes with different fluorescent spectra. (cdc.gov)
  • Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)
  • During RT, alterations in signaling pathways result in changes in the local immune microenvironment. (nature.com)
  • This work package utilises our large islet RNA data sets as well as a new human islet cell encyclopedia generated by single-cell RNA sequencing, and focuses on miRNA translation pathways, membrane receptor and intracellular Ca2+ signalling pathways. (lu.se)
  • It uses rolling-circle amplification of RNAs to achieve a high signal/noise ratio. (sinica.edu.tw)
  • With this dual signal amplification platform, as low as 1 × 102 particles per μL exosomes could be detected. (exosome-rna.com)
  • The hybridization signals for each probe when a nucleic abnormality is detected. (wikipedia.org)
  • After checking all the necessary conditions, hybridization steps can be started by first adding a target-specific probe, composed of 20 oligonucleotide pairs, hybridizes to the target RNA(s). (wikipedia.org)
  • Signal amplification is achieved via series of sequential hybridization steps. (wikipedia.org)
  • Yes, CF® dye tyramides can be used for in situ hybridization using Tyramide Signal Amplification 1 (TSA). (biotium.com)
  • The degree of homology between target and probe results in stable hybridization. (enzolifesciences.com)
  • And this strategy was named as high- temperature hybridization chain reaction coupled with strand displacement amplification (HSA). (bvsalud.org)
  • And the trigger can be identified by a high- temperature hybridization chain reaction and initiate a secondary signal amplification. (bvsalud.org)
  • An ultrahigh-throughput single-cell miRNA assay is developed by a continuous-flow microfluidic process employing isothermal amplification to amplify the target miRNA signal. (rsc.org)
  • FISH can also be used to detect and localize specific RNA targets (mRNA, lncRNA and miRNA)[citation needed] in cells, circulating tumor cells, and tissue samples. (wikipedia.org)
  • In biological study, one sometimes needs to detect the simultaneous presence of a micro RNA (miRNA) and its putative target mRNA in the same cell. (sinica.edu.tw)
  • If the target miRNA exists, RRO probe can initiate a strand displacement amplification and output a target-related special single-stranded DNA (trigger). (bvsalud.org)
  • In terms of mechanism, the functional gain and loss experiments of human LEC show that TLR3 regulates the proliferation, migration, invasion of LEC and EMT induced by TGF-β2 by targeting the Jagged-1/Notch signaling pathway to affect the development of fibrotic cataract. (researchsquare.com)
  • These findings indicate that TLR3 prevents the progression of lens fibrosis by targeting the Jagged-1/Notch signaling pathway to regulate LEC proliferation, migration, invasion and TGF-β2-induced EMT. (researchsquare.com)
  • On the biochemistry function, POGLUT2 can catalyze to transfer the glucose from UDP-glucose to a serine residue and specifically target extracellular epidermal growth factor (EGF) repeats of proteins such as Notch signaling members (NOTCH1 and NOTCH3) ( 9 , 10 ), thereby affecting Notch signaling pathway ( 11 ). (frontiersin.org)
  • I n an attempt to improve behavioral memory, we devised a strategy to amplify the signal-to-noise ratio of the cAMP pathway, which plays a central role in hippocampal synaptic plasticity and behavioral memory. (biopsychiatry.com)
  • Furthermore, in addition to electrode materials, functional nanomaterials can not only produce a synergic effect among catalytic activity, conductivity, and biocompatibility to accelerate the signal transduction but also amplify biorecognition events with specifically designed signal tags, leading to highly sensitive biosensing. (cdc.gov)
  • Labeled antibodies against several important biological target proteins are commercially available. (bdbiosciences.com)
  • Our Tyramide Amplification Kits have been demonstrated to be robust and versatile for multi-color fluorescence imaging, compatible with dye-labeled antibodies and various cell staining methods (see Figure 1). (biotium.com)
  • In a typical experiment, microfluidics are used to spatially control chip functionalization by directing capture antibodies against a variety of protein targets to groups of microring sensors. (illinois.edu)
  • They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. (thermofisher.com)
  • Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. (thermofisher.com)
  • Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway. (enzolifesciences.com)
  • Cytokines are small signaling proteins that are present in serum and cell secretomes at concentrations in the pg/mL or ng/mL range. (illinois.edu)
  • Provides marked enhancement of antigen-associated fluorescence signal. (cellsignal.com)
  • The covalent nature of tyramide-tyrosine engagement allows for heat-mediated removal (stripping) of primary/secondary antibody pairs bound to the antigen, while preserving the antigen-associated fluorescence signal. (cellsignal.com)
  • If the target sequence is present, the probe anneals downstream from one of the primer sites and is cleaved by the 5´ nuclease activity of Taq DNA polymerase as this primer is extended. (thermofisher.com)
  • Removes the probe from the target strand, allowing primer extension to continue to the end of the template strand. (thermofisher.com)
  • Specimens containing the target DNA hybridize with the HR HPV RNA probe cocktail. (cdc.gov)
  • The Luna Probe One-Step RT-qPCR Kit (No ROX) is optimized for real-time quantitation of target RNA sequences using hydrolysis probes. (neb.com)
  • Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5´ → 3´ exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. (neb.com)
  • In HSA, we well designed a target signal recognition, replication, and output probe (RRO probe). (bvsalud.org)
  • The aim of this study was to investigate whether resveratrol (RSV) could ameliorate ischemia- and hypoxia-associated cardiomyocyte apoptosis and injury via inhibiting senescence signaling and inflammasome activation. (hindawi.com)
  • The p53-associated signaling pathway has been demonstrated to regulate hypoxia-induced apoptosis and senescence [ 5 ]. (hindawi.com)
  • Multispectral analysis software addresses this issue by subtracting the artifactual fluorescence signal that originates from unstained tissue. (cellsignal.com)
  • At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle or C q value can be determined. (neb.com)
  • In real-time PCR, the DNA amplification is monitored by measuring the fluorescence signal generated in the reaction vessel. (cdc.gov)
  • The fluorescence signal is measured every cycle and is proportional to the amount of accumulated PCR product. (cdc.gov)
  • As the reaction proceeds and PCR product accumulates, the amount of double-stranded DNA increases and with it the fluorescence signal (4-5). (cdc.gov)
  • It amplifies the signal (its target) and the noise (anything in the background). (planetwaves.net)
  • With remarkable achievements in nanotechnology and nanoscience, nanomaterial-based electrochemical signal amplifications have great potential of improving both sensitivity and selectivity for electrochemical sensors and biosensors. (cdc.gov)
  • Expression, gene mutation and amplification, methylation, and copy number alteration (CNA) of POGLUT2 were evaluated using The Cancer Genome Atlas (TCGA), Cancer Cell Line Encyclopedia (CCLE), and Genotype-Tissue Expression (GTEx) databases. (frontiersin.org)
  • HC-2 can detect as little as 1 pg/mL of target DNA (5000 genome copies per test). (medscape.com)
  • The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. (neb.com)
  • At the start of the reaction, very little double stranded DNA is present, and so the fluorescent signal detected by the thermocycler is low (3). (cdc.gov)
  • The coagulation cascade is the most prevalent target for these drugs. (chemdiv.com)
  • It is becoming increasingly clear that exosomes have specialized functions and play a key role in, for example, coagulation, intercellular signaling, and waste management. (exosome-rna.com)
  • Morphogen gradients induce sharply defined domains of gene expression in a concentration-dependent manner, yet how cells interpret these signals in the face of spatial and temporal noise remains unclear. (elifesciences.org)
  • Results: A QPCR (Fluidigm) and a barcoded oligo-based (NanoString) gene expression platform were compared for evaluation of amplification of gene expression signal for 19 genes from degraded RNA extracted from FFPE biopsy sections by a set protocol. (escholarship.org)
  • Conclusions: Target gene expression amplification across a custom set of genes can identify AR independent of histology, and quantify inflammation from archival kidney transplant biopsy tissue, providing a new tool for clinical correlation and outcome analysis of kidney allografts, without the need for prospective kidney biopsy biobanking efforts. (escholarship.org)
  • RSV has also been found to regulate other molecular targets such as AMP-activated protein kinase, nuclear factor erythroid 2-related factor 2, nuclear factor- (NF-) κ B, and endothelial nitric oxide synthase and has also been considered to have powerful antioxidant and anti-inflammatory properties. (hindawi.com)
  • However, recent studies have suggested that BRAFi/MEKi and ERK1/2i resistance can arise through activation of a parallel signalling pathway leading to activation of ERK5, an unusual protein kinase that contains both a kinase domain and a transcriptional transactivation domain. (babraham.ac.uk)
  • Studies using pharmacological inhibitors and genetic manipulations have shown that this difference in response depends on the activity of cAMP-dependent protein kinase A. Genetic studies have also indicated that protein kinase A and one of its target transcription factors, cAMP response element binding protein, are important in memory in vivo. (biopsychiatry.com)
  • The availability of these fluorogenic probes enabled the development of a real-time method for detecting only specific amplification products. (thermofisher.com)
  • Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
  • We conclude that further development of targeted approaches should prevent inaccurate conclusions about the nature of biological regulations and in particular kinase-substrate networks. (acs.org)
  • Using fluorescence lifetime imaging microscopy (FLIM) and phasor analysis to measure endogenous retinoic acid (RA) directly in vivo, we have investigated the amplitude of noise in RA signaling, and how modulation of this noise affects patterning of hindbrain segments (rhombomeres) in the zebrafish embryo. (elifesciences.org)
  • The purpose of using RNA FISH is to detect target mRNA transcripts in cells, tissue sections, or even whole-mounts. (wikipedia.org)
  • Understand co-expression and spatial organization of multiple targets within preserved tissue architecture, unlike alternative multiplex approaches (e.g., next generation sequencing, PCR, mass spectrometry, etc. (cellsignal.com)
  • Cells responding to the signal somehow can ignore this noise and establish sharp boundaries between different cell types so that neighboring cells have distinct roles in the tissue. (elifesciences.org)
  • ISH confirmed a high degree of correlation of signal amplification and tissue localization. (escholarship.org)
  • Currently used analytical methods are deficient in that they often provide either highly quantitative data for a single biomarker or qualitative data for many targets, but methods that simultaneously provide highly quantitative analysis of many targets have yet to be adequately developed. (illinois.edu)
  • Separate but compatible signal amplification systems enable the multiplex assay (up to two targets per assay). (wikipedia.org)
  • These PCR-based and PCR-free experiments demonstrate that, overall, PCR amplification does not significantly bias the results of TnSeq insofar as insertions in the majority of genes represented in our library were similarly detected regardless of PCR cycle number and whether or not PCR amplification was employed. (microbiologyresearch.org)
  • We conclude that PCR-based enrichment of transposon insertions in a TnSeq assay is reliable, but researchers interested in profiling putative essential genes should carefully weigh the number of amplification cycles employed in their library preparation protocols. (microbiologyresearch.org)
  • The method is based on a magnetic barcoding strategy: PCR-amplified target MTB genes are captured and magnetically labeled by a pair of complementary oligonucleotides conjugated to microspheres and magnetic nanoprobes. (cdc.gov)
  • Activation of Hedgehog signaling by the environmental toxicant arsenic may contribute to the etiology of arsenic-induced tumors. (mbl.edu)
  • Activation-- The process of inducing radioactivity by neutron irradiation of a target material. (cdc.gov)
  • To test the hypothesis that escape from G1 arrest in drug promotes amplification, we exploited the combination of palbociclib and selumetinib. (babraham.ac.uk)
  • A device that uses specific biochemical reactions mediated by isolated enzymes, immunosystems, tissues, organelles or whole cells to detect chemical compounds, usually by electrical, thermal or optical signals. (genomicglossaries.com)
  • The mechanisms of POGLUT2 in breast cancer were briefly evaluated via its connection with Notch signaling. (frontiersin.org)
  • These resistance mechanisms frequently involve reinstatement of ERK1/2 signalling and BRAFi are now deployed in combination with one of three approved MEK1/2 inhibitors (MEKi) to provide more durable, but still transient, clinical responses. (babraham.ac.uk)
  • Cells responding to signals need to be able to distinguish these signals from random fluctuations (i.e., noise) and presumably have evolved mechanisms to do so. (elifesciences.org)
  • In this work package we aim to examine underlying mechanisms of to find possible pharmacological targets to be used in personalised treatment. (lu.se)
  • Each nanoparticle subsequently produces an electronic signal that enables precise measurement of their electric charge in low concentrations and small volumes. (acs.org)
  • Sur 60 hémocultures positives pour les cocci à Gram positif en grappes, l'amplification isotherme induite par boucle (au moyen du dépistage des gènes Fem A et Mec A) a montré une sensibilité et une spécificité de 100 % pour l'identification de Staphylococcus aureus résistant et sensible à la méthicilline. (who.int)
  • Increased levels of POGLUT2 could result in the dysregulated immune cell infiltration and tumor microenvironment and showed a significant regulatory effect on the progression of breast cancer through Notch-related signaling. (frontiersin.org)
  • Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)
  • Our findings demonstrate that acquisition of MEK inhibitor resistance often occurs through gene amplification and can be suppressed by impeding cell cycle entry in drug. (babraham.ac.uk)
  • Species-specificity for C. psittaci and C. pecorum LAMP targets was tested against DNA samples from related chlamydial species and a range of other bacteria. (peerj.com)
  • As with amplification, turn it up high enough and the signal and the noise blend into one thing. (planetwaves.net)
  • Few studies have attempted to measure such noise or address how cells manage to respond to noisy signals in a consistent manner. (elifesciences.org)
  • In each case, binding of analytes to the rings causes a change in the local refractive index that is transduced into a real-time, quantitative optical signal. (illinois.edu)
  • In real-time PCR (qPCR), reactions are characterized by the point in time during cycling when amplification of a target is first detected rather than the amount of target accumulated after a fixed number of cycles. (thermofisher.com)
  • PKCι Is a Promising Prognosis Biomarker and Therapeutic Target for Pancreatic Cancer. (nih.gov)
  • TLR3-Jagged-1/Notch' signal axis may be a therapeutic target for the treatment of fibrotic cataract. (researchsquare.com)
  • This research provides critical new insights regarding SERS signal variation and offers a simple methodology for reliable determination of the SERS enhancement factors. (acs.org)
  • C q values can be used to evaluate relative target abundance between two or more samples, or to calculate absolute target quantities in reference to an appropriate standard curve derived from a series of known dilutions. (neb.com)
  • Being able to visualize multiple targets simultaneously is important in research fields like tumor immunology where it is necessary to catalog subsets of immune and cancer cells within the tumor microenvironment. (cellsignal.com)
  • By transmitting signals from space directly to receiving antennas on Earth, satellites can provide coverage over vast distances without being hindered by physical obstacles or expensive infrastructure development. (mabuhaysat.com)
  • Retinoic acid is a signaling molecule that plays an important role in the development of the brain in animal embryos. (elifesciences.org)
  • This affinity and complementary sequence allow binding to specific regions of a target sequence of nucleotides. (enzolifesciences.com)
  • Therefore, specific signals recognition and output in nucleic acid amplification are crucial to reliability of clinical diagnosis . (bvsalud.org)