SiglecsReceptorsAntigenAlpha-2,6-linked sialicMediates B-cellProteinSuperfamilyReceptorExtracellularTransmembraneGlycansLigandMoleculesELISAProteinsSialoadhesinMoleculeCharacterizationGlycanLymphoidRecombinantMonoclonalAntibodyAmino AcidsMicrogliaAcute myeloidHumanRecognitionConcentrationIgG1DomainInteractionsImmune systemEvolutionInteractionCellReduction
Siglecs12
- Sialic acid-binding Immunoglobulin-like lectins (Siglecs) are cell-surface I-type lectins that act as immunomodulatory sialic acid-binding receptors. (medchemexpress.com)
- Siglecs belong to the Immunoglobulin (Ig) superfamily. (medchemexpress.com)
- All Siglecs are single-pass type 1 integral membrane proteins containing extracellular domains with one (or two, in the case of Siglec-12) unique and homologous N-terminal V-set Ig domain, followed by variable numbers of C2-set Ig domains, ranging from 16 in sialoadhesin to 1 in CD33. (medchemexpress.com)
- Based on sequence homologies,the Siglecs can be divided into two groups: one including all CD33-related Siglecs and the other consisting of Siglec-1 (Sialoadhesin), Siglec-2 (CD22), Siglec-4 (myelin-associated glycoprotein, MAG) and Siglec-15. (medchemexpress.com)
- Zum anderen ist CD22 ein Adhäsionsmolekül, das in die Gruppe der Siglecs (Sialic acid-binding Ig-like lectin) gehört. (uni-wuerzburg.de)
- Siglecs are integral membrane proteins with extracellular N-terminal, V-set Ig domains, followed by variable numbers of C2-set Ig domains. (bdbiosciences.com)
- Crocker PR, Varki A. Siglecs, sialic acids and innate immunity. (bdbiosciences.com)
- Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. (kactusbio.com)
- Siglecs are cell surface proteins that bind sialic acid. (transcriptionfactor.org)
- Siglec 9 and siglec 6-8,10-12 are CD33 (siglec 3) like siglecs, which have two ITIMs in the cytoplasmic tails, suggesting their functional involvement in signal transduction. (biolegend.com)
- The crystal structure of the N-terminal immunoglobulin-like domain of the Siglec sialoadhesin (SnD1) in complex with 2,3-sialyllactose has informed the design of sialic acid analogs (sialosides) that bind Siglecs with significantly enhanced affinities and specificities. (ox.ac.uk)
- Myeloid cell surface antigen CD33 also known as SIGLEC3, Siglecs (sialic acid binding Ig-like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. (biovisi.com)
Receptors5
- It interacts with SIALIC ACIDS and mediates signaling from B-CELL ANTIGEN RECEPTORS. (bvsalud.org)
- Since sialic acids can trigger immune inhibitory sialic acid-binding Ig-like lectin (Siglec) receptors, we screened for Siglec ligands on the EVs. (amsterdamumc.org)
- The efficacy of a therapeutic antibody depends on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors.Therefore, candidates must be tested against a panel of receptors during antibody engineering. (acrobiosystems.com)
- Paired Siglec receptors generate opposite inflammatory responses to a human-specific pathogen. (unige.ch)
- The Siglec family of receptors mediates cell surface interactions through recognition of sialylated glycoconjugates. (ox.ac.uk)
Antigen7
- B-cell receptor CD22 is also known as Sialic acid-binding Ig-like lectin 2 (Siglec-2), B-lymphocyte cell adhesion molecule (BL-CAM), T-cell surface antigen Leu-14, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. (dimabio.cn)
- The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g. (elabscience.com)
- It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. (elabscience.com)
- By binding to the CD33 antigen on tumors, the cytotoxic agent blocks the growth of cancerous cells and causes cell death. (sydlabs.com)
- Binding of the anti-CD33 antibody portion of Gemtuzumab with the CD33 antigen results in the formation of a complex that is internalized. (sydlabs.com)
- They exist in pentameric form (IgM), dimeric form (IgA) or single form (IgG, IgE, IgD), which consist of Y-shaped units that each have two antigen-binding pockets on one side and a region recognized by immune cells on the opposite. (anthropogeny.org)
- BCRs allow the B-cell to bind to a specific antigen , against which it will initiate an antibody response. (anthropogeny.org)
Alpha-2,6-linked sialic2
- Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. (kactusbio.com)
- Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. (cusabio.com)
Mediates B-cell1
- Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. (elabscience.com)
Protein14
- Sialic acid binding Ig-like lectin 15 is a protein that in humans is encoded by the SIGLEC15 gene. (wikipedia.org)
- Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. (creativebiolabs.net)
- DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. (creativebiolabs.net)
- After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. (creativebiolabs.net)
- Recombinant Biotinylated Human Siglec-5/CD170 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu17-Thr434. (kactusbio.com)
- ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD22, hFc-His tagged protein (PME100005) can bind Anti-CD22 Neutralizing antibody ( BME100029 ) in a linear range of 3.2-400 ng/ml. (dimabio.cn)
- Polysialic acid (polySia) is a polymeric glycan with variable chain lengths, presented as posttranslational modification on select protein carriers. (for2953-sia.de)
- As shown recently, polySia-modified proteins can also be released by activated microglia, and the intrinsically released protein-bound and exogenously applied free polySia were equally able to attenuate proinflammatory microglia activation via the inhibitory immune receptor Siglec-E. Here, we explore polySia as a candidate substance for promoting myelin regeneration by immunomodulation. (for2953-sia.de)
- CD22 molecule is a transmembrane protein from SIGLEC (Sialic acid-binding immunoglobulin-type lectins) family. (biomedcentral.com)
- Streptavidin is a tetrameric protein providing 4 high-affinity biotin binding sites. (acrobiosystems.com)
- Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5. (anthropogeny.org)
- The in vivo grade gemtuzumab biosimilar specifically binds to the human CD33 protein. (sydlabs.com)
- These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. (cusabio.com)
- Human Siglec-10 can bind to vascular adhesion protein-1 and serves as its substrate. (genome.jp)
Superfamily2
- They are a family of carbohydrate binding proteins within the immunoglobulin superfamily. (bdbiosciences.com)
- Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. (transcriptionfactor.org)
Receptor5
- Once bind, the toxin enters cells by receptor-mediated endocytosis. (creativebiolabs.net)
- Surprisingly, however, glioblastoma EVs lack glycans that could bind Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin (DC-SIGN, CD209), a receptor that mediates uptake and induction of CD4+ and CD8+ T cell activation. (amsterdamumc.org)
- Siglec-14 is a sialic acid-recognizing activatory receptor found on certain immune cells types that is undergoing concerted evolution with the gene for Siglec-5 (an inhibitory receptor) via ongoing gene conversion of exons encoding amino-terminal V-set lg-like domains. (anthropogeny.org)
- Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. (anthropogeny.org)
- In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 Domain (s) that block signal transduction through dephosphorylation of signaling molecules. (biovisi.com)
Extracellular1
- The extracellular domain of siglec-9 contains an IgV region, which binds sialic acid, followed by two IgC regions. (biolegend.com)
Transmembrane2
- Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail. (transcriptionfactor.org)
- Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N--terminal Ig--like V--type domain, one Ig--like C2--type domains, a transmembrane region and a cytoplasmic tail. (biovisi.com)
Glycans2
- The surface glycan profile of human glioblastoma cell line-derived EVs (50-200 nm) was dominated by α-2,3- and α-2,6 linked sialic acid-capped complex N-glycans and bi-antennary N-glycans. (amsterdamumc.org)
- Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. (transcriptionfactor.org)
Ligand1
- The tyrosine residues of the ITIMs become phosphorylated with the ligand binding, which leads to activation of SHP-1 ( Src homology region 2 domain-containing phosphatase-1), SHP-2 ( Src homology region 2 domain-containing phosphatase-2), SHIP-1 (( Src homology region 2 domain-containing inositol 5′ polyphosphatase-1). (biomedcentral.com)
Molecules2
- The result is that the two molecules have similar binding properties for sialic acids. (anthropogeny.org)
- It appears that this may be the ancestral condition, and that the human molecules have restored the arginine residue required for sialic acid recognition. (anthropogeny.org)
ELISA2
- Here, we set out to characterize the glycocalyx composition of glioblastoma EVs by lectin-binding ELISA and comprehensive immunogold transmission electron microscopy (immuno-TEM). (amsterdamumc.org)
- Additional reported applications (for the relevant formats) include: immunofluorescence staining 2 , Western blotting 2 , immunoprecipitation 2 , and ELISA 3 . (biolegend.com)
Proteins1
- Polymeric sialic acid (polysialic acid, polySia) is a remarkable posttranslational modification of only few select proteins. (for2953-sia.de)
Sialoadhesin1
- Structure-guided design of sialic acid-based Siglec inhibitors and crystallographic analysis in complex with sialoadhesin. (ox.ac.uk)
Molecule3
- Siglec 5 is putative adhesion molecule that mediates sialic-acid dependent binding to cells. (kactusbio.com)
- A lectin and cell adhesion molecule found in B-LYMPHOCYTES. (bvsalud.org)
- A recently evolved signaling molecule that can interact with SHP-1 and SHP-2 and is expressed by tissue macrophages, including brain microglia. (unige.ch)
Characterization4
- Angata T, Hingorani R, Varki NM, Varki A. Cloning and characterization of a novel mouse Siglec, mSiglec-F: differential evolution of the mouse and human (CD33) Siglec-3-related gene clusters. (bdbiosciences.com)
- Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes. (bdbiosciences.com)
- Cloning and characterization of human Siglec-11. (unige.ch)
- Molecular characterization, tissue expression, and mapping of a novel Siglec-like gene (SLG2) with three splice variants. (genome.jp)
Glycan1
- Therefore, we explored whether modification of the EV glycan surface could reduce immune inhibitory Siglec binding, while enhancing EV internalization by DCs in a DC-SIGN dependent manner. (amsterdamumc.org)
Lymphoid1
- CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. (biovisi.com)
Recombinant1
- SARS-CoV-2 Mutant (L452R, T478K)Alternates names: N/ATaglines: Recombinant Spike RBD mutant of the SARS-CoV-2. (transcriptionfactor.org)
Monoclonal1
- The F023-420 monoclonal antibody specifically recognizes CD328 which is also known as sialic acid binding Ig like lectin 7 (Siglec-7). (bdbiosciences.com)
Antibody4
- Standards and samples are pipetted into the wells and MAG present in a sample is bound to the wells by the immobilized antibody. (raybiotech.com)
- The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. (bdbiosciences.com)
- Setting the corresponding isotype control antibody to detect non-specific binding can reduce the generation of false positive results and evaluate the possible influencing factors accurately in the drug development process. (acrobiosystems.com)
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. (biolegend.com)
Amino Acids1
- Amino acids are encoded by the genome as different three nucleotide codes. (anthropogeny.org)
Microglia3
- Based on determination of DP24 as the minimal polySia chain length required for inhibition of inflammatory BV-2 microglia activation, pools with short and long polySia chains (DP8-14 and DP24-30) were generated and applied to slice cultures during remyelination. (for2953-sia.de)
- Unlike DP8-14, treatment with DP24-30 significantly improved remyelination, increased arginase-1-positive microglia ratios, and reduced the production of nitric oxide in wildtype, but not Siglec-E-deficient slice cultures. (for2953-sia.de)
- Collectively, these results suggest a beneficial effect of exogenously applied polySia DP24-30 on remyelination by Siglec-E-dependent microglia regulation. (for2953-sia.de)
Acute myeloid1
- CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia. (biovisi.com)
Human4
- Profile of human Siglec-7 (clone F023-420) reactivity on peripheral blood lymphocytes analyzed by flow cytometry. (bdbiosciences.com)
- The purity of Biotinylated Human Siglec-5 is greater than 95% as determined by SEC-HPLC. (kactusbio.com)
- Biotinylated Human Siglec-5 on Tris-Bis PAGE under reduced condition. (kactusbio.com)
- A second uniquely human mutation affecting sialic acid biology. (unige.ch)
Recognition1
- The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. (kactusbio.com)
Concentration1
- It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH 2 O. (medchemexpress.com)
IgG12
- No. 426103) and Siglec-9 (clone K8) PerCP/Cyanine5.5 (left) or mouse IgG1, κ isotype control PerCP/Cyanine5.5 (right). (biolegend.com)
- No. 426103) and Siglec-9 (clone K8) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ isotype control PerCP/Cyanine5.5 (open histogram). (biolegend.com)
Domain3
- They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. (kactusbio.com)
- It has three parts: (i) V-type Ig domain with sia -binding site, (ii) C1-type Ig domain, (iii) C2-type Ig domain. (biomedcentral.com)
- Most PWS and AS cases (~70%) are due to ~5 Mb de novo deletions spanning a 2 Mb imprinted domain and several adjacent non-imprinted genes [ 1 ]. (biomedcentral.com)
Interactions1
- Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. (cusabio.com)
Immune system1
- Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates. (transcriptionfactor.org)
Evolution1
- Evolution of siglec-11 and siglec-16 genes in hominins. (unige.ch)
Interaction1
- Possible selection pressures include the interaction with sialic acid-expressing pathogens and general control of innate immune responses. (anthropogeny.org)
Cell2
- B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb. (elabscience.com)
- Siglec-9 plays a role in negative regulation of T cell activation, and it also affects neutrophil apoptosis. (biolegend.com)
Reduction1
- Desialylation with a pan-sialic acid hydrolase led to reduction of sialic acid expression on EVs. (amsterdamumc.org)