Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. (wikipedia.org)
Zum anderen ist CD22 ein Adhäsionsmolekül, das in die Gruppe der Siglecs (Sialic acid-binding Ig-like lectin) gehört. (uni-wuerzburg.de)
Sialic acid-binding Immunoglobulin-like lectins (Siglecs) are cell-surface I-type lectins that act as immunomodulatory sialic acid-binding receptors. (medchemexpress.com)
Siglecs belong to the Immunoglobulin (Ig) superfamily. (medchemexpress.com)
All Siglecs are single-pass type 1 integral membrane proteins containing extracellular domains with one (or two, in the case of Siglec-12) unique and homologous N-terminal V-set Ig domain, followed by variable numbers of C2-set Ig domains, ranging from 16 in sialoadhesin to 1 in CD33. (medchemexpress.com)
Based on sequence homologies,the Siglecs can be divided into two groups: one including all CD33-related Siglecs and the other consisting of Siglec-1 (Sialoadhesin), Siglec-2 (CD22), Siglec-4 (myelin-associated glycoprotein, MAG) and Siglec-15. (medchemexpress.com)
Siglecs are integral membrane proteins with extracellular N-terminal, V-set Ig domains, followed by variable numbers of C2-set Ig domains. (bdbiosciences.com)
The crystal structure of the N-terminal immunoglobulin-like domain of the Siglec sialoadhesin (SnD1) in complex with 2,3-sialyllactose has informed the design of sialic acid analogs (sialosides) that bind Siglecs with significantly enhanced affinities and specificities. (ox.ac.uk)
It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. (kactusbio.com)
Sialic acid-binding Ig-like lectin 12, or Siglec-XII, is a protein that in humans, is encoded by the SIGLEC12 gene. (wikipedia.org)
Here we identified a subset of HLA-DR+ CD14+ CD11c+ cervical DCs at the lamina propria of the ectocervix and the endocervix that expressed the type-I interferon inducible lectin Siglec-1 (CD169), which promoted viral uptake. (gencat.cat)
In the cervical biopsy of a viremic HIV-1+ patient, Siglec-1+ cells harbored HIV-1-containing compartments, demonstrating that in vivo, these cells trap viruses. (gencat.cat)
Ex vivo, a type-I interferon antiviral environment enhanced viral capture and trans-infection via Siglec-1. (gencat.cat)
Nonetheless, HIV-1 transfer via cervical DCs was effectively prevented with antibodies against Siglec-1. (gencat.cat)
Our findings contribute to decipher how cervical DCs may boost HIV-1 replication and promote systemic viral spread from the cervical mucosa, and highlight the importance of including inhibitors against Siglec-1 in microbicidal strategies. (gencat.cat)
The F023-420 monoclonal antibody specifically recognizes CD328 which is also known as sialic acid binding Ig like lectin 7 (Siglec-7). (bdbiosciences.com)
CD22 molecule is a transmembrane protein from SIGLEC (Sialic acid-binding immunoglobulin-type lectins) family. (biomedcentral.com)
Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. (elabscience.com)
The extracellular domain of siglec-9 contains an IgV region, which binds sialic acid, followed by two IgC regions. (biolegend.com)
Structure-guided design of sialic acid-based Siglec inhibitors and crystallographic analysis in complex with sialoadhesin. (ox.ac.uk)
Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. (fishersci.com)
The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. (fishersci.com)
Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. (transcriptionfactor.org)
Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail. (transcriptionfactor.org)
B-cell receptor CD22 is also known as Sialic acid-binding Ig-like lectin 2 (Siglec-2), B-lymphocyte cell adhesion molecule (BL-CAM), T-cell surface antigen Leu-14, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. (dimabio.cn)
Since sialic acids can trigger immune inhibitory sialic acid-binding Ig-like lectin (Siglec) receptors, we screened for Siglec ligands on the EVs. (amsterdamumc.org)
Glioblastoma EVs showed significant binding to Siglec-9, which is highly expressed on DCs. (amsterdamumc.org)
Therefore, we explored whether modification of the EV glycan surface could reduce immune inhibitory Siglec binding, while enhancing EV internalization by DCs in a DC-SIGN dependent manner. (amsterdamumc.org)
Antigen5
Antigen presenting cells from the cervical mucosa are thought to amplify incoming HIV-1 and spread infection systemically without being productively infected. (gencat.cat)
The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g. (elabscience.com)
It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. (elabscience.com)
By binding to the CD33 antigen on tumors, the cytotoxic agent blocks the growth of cancerous cells and causes cell death. (sydlabs.com)
Binding of the anti-CD33 antibody portion of Gemtuzumab with the CD33 antigen results in the formation of a complex that is internalized. (sydlabs.com)
Immunoglobulin superfamily1
They are a family of carbohydrate binding proteins within the immunoglobulin superfamily. (bdbiosciences.com)
Mediates4
It mediates negative impact on BCR signaling pathway by dephosphorylating the associated cascade components via protein tyrosine phosphatases [ 67 , 68 , 69 ] (Fig. 1 ). (biomedcentral.com)
SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. (fishersci.com)
They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. (kactusbio.com)
Surprisingly, however, glioblastoma EVs lack glycans that could bind Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin (DC-SIGN, CD209), a receptor that mediates uptake and induction of CD4+ and CD8+ T cell activation. (amsterdamumc.org)
Receptors1
They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein. (bvsalud.org)
Amino acids2
Description: SIGLEC6 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 563 amino acids (27-347a.a.) and having a molecular mass of 62.6kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). (therabio.org)
SIGLEC6 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques. (therabio.org)
Receptor9
Consistent with immunological imprinting, most antibodies derived from memory B cells or plasma cells of Omicron breakthrough cases cross-react with the Wuhan-Hu-1, BA.1 and BA.2 receptor-binding domains whereas Omicron primary infections elicit B cells of narrow specificity. (bvsalud.org)
Here, we tested a panel of anti-receptor binding domain monoclonal antibodies (mAbs) corresponding to those in clinical use by Vir Biotechnology (S309, the parent mAb of VIR-7831 [Sotrovimab]), AstraZeneca (COV2-2196 and COV2-2130, the parent mAbs of AZD8895 and AZD1061), Regeneron (REGN10933 and REGN10987), Lilly (LY-CoV555 and LY-CoV016), and Celltrion (CT-P59) for their ability to neutralize an infectious B.1.1.529 Omicron isolate. (bvsalud.org)
The recently emerged SARS-CoV-2 Omicron variant harbors 37 amino acid substitutions in the spike (S) protein, 15 of which are in the receptor-binding domain (RBD), thereby raising concerns about the effectiveness of available vaccines and antibody therapeutics. (bvsalud.org)
Most (26 out of 29) receptor-binding motif (RBM)-directed monoclonal antibodies (mAbs) lost in vitro neutralizing activity against Omicron, with only three mAbs, including the ACE2-mimicking S2K146 mAb, retaining unaltered potency. (bvsalud.org)
Broad-scale unbiased genomic studies bring several genetic variants such as sialic acid binding Ig-like lectin 3 (CD33), triggering receptor expressed on myeloid cells 2 (TREM2) or complement receptor type 1 (CR1) into the focus of researchers' attention as potential risk factors for neuroinflammation. (cannabisclinicians.org)
The most common example of this type of fusion protein combines the hinge and Fc regions of an immunoglobulin (Ig) with domains of a cell-surface receptor that recognizes a specific ligand (Box 1). (coek.info)
Immunoadhesin structure The majority of immunoadhesins combine the hinge and Fc regions of an IgGl heavy chain with the extracellular domain (ECU) of a type I transmembrane protein, usually a receptor or an adhesion molecule (Fig. 1). (coek.info)
A recently evolved signaling molecule that can interact with SHP-1 and SHP-2 and is expressed by tissue macrophages, including brain microglia. (unige.ch)
CD2214
CD22 ist ein B-zellspezifisches Transmembranprotein der Immunglobulin (Ig)-Superfamilie. (uni-wuerzburg.de)
Nach BZR-Ligation lagert sich die Tyrosin-Phosphatase SHP-1 an die cytoplasmatische Domäne von CD22 an, wodurch SHP-1 aktiviert wird. (uni-wuerzburg.de)
Die N-terminale Ig-Domäne von CD22 weist die Bindungseigenschaften eines Lectins auf und bindet spezifisch 2,6-gekoppelte Sialinsäure. (uni-wuerzburg.de)
Daher wurde das Substrat der an CD22 rekrutierten und aktivierten Phosphatase SHP-1 in vergleichenden Analysen von CD22-defizienten und Kontroll-B-Zellen nach BZR-Stimulation gesucht. (uni-wuerzburg.de)
Transfektionsexperimente mit der CD22-defizienten Plasmazytom-Zelllinie J558Lm3 wurden begonnen, um den molekularen Zusammenhang zwischen CD22, SHP-1 und SLP-65/BLNK zu bestätigen. (uni-wuerzburg.de)
Das in J558Lm3 Zellen ektopisch exprimierte CD22 wurde Tyrosin-phosphoryliert, und SHP-1 konnte mit CD22 co-präzipitiert werden. (uni-wuerzburg.de)
Diese Ergebnisse verdeutlichten, dass das Adaptermolekül SLP-65/BLNK zwar ein Substrat des CD22/SHP-1 Signalweges ist, aber keine essentielle Rolle in der inhibitorischen Wirkung von CD22 übernimmt. (uni-wuerzburg.de)
Entsprechend war die Tyrosin-Phosphorylierung von CD22 nach Vorbehandlung mit dem Sialosid in den humanen B-Zellen verringert, und weniger SHP-1 konnte mit CD22 co-präzipitiert werden. (uni-wuerzburg.de)
Als Nebenprojekt wurde die Rolle von CD22 in knock-out Mäusen des transkriptionellen Co-Aktivators BOB.1/OBF.1 untersucht. (uni-wuerzburg.de)
Recombinant Mouse Antibody Fab Fragment is capable of binding to Human CD22, expressed in Chinese Hamster Ovary cells(CHO). (creativebiolabs.net)
Polygenic autoimmune traits: Lyn, CD22, and SHP-1 are limiting elements of a biochemical pathway regulating BCR signaling and selection. (ox.ac.uk)
As in invertebrates, genetic effects of loci with only one functional allele can be used to analyze signaling networks in mice, demonstrating that negative regulation of the BCR is a complex quantitative trait in which Lyn, the coreceptor CD22, and the tyrosine phosphatase SHP-1 are each limiting elements. (ox.ac.uk)
The biochemical basis of this complex trait involves a pathway requiring Lyn to phosphorylate CD22 and recruit SHP-1 to the CD22/BCR complex. (ox.ac.uk)
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD22, hFc-His tagged protein (PME100005) can bind Anti-CD22 Neutralizing antibody ( BME100029 ) in a linear range of 3.2-400 ng/ml. (dimabio.cn)
Extracellular1
Journal of extracellular vesicles , 8 (1), Article 1648995. (amsterdamumc.org)
ELISA9
Description: A sandwich ELISA kit for detection of Sialic Acid Binding Ig Like Lectin 6 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (therabio.org)
Description: A sandwich quantitative ELISA assay kit for detection of Human Sialic Acid Binding Ig Like Lectin 10 (SIGLEC10) in samples from serum, plasma, tissue homogenates or other biological fluids. (therabio.org)
Description: A sandwich ELISA kit for detection of Hepatocyte Nuclear Factor 1 Alpha from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (wildpalm.net)
Description: Quantitativesandwich ELISA kit for measuring Rat Hepatocyte nuclear factor 1-alpha (HNF1A) in samples from serum, plasma, cell culture supernates, tissue homogenates. (wildpalm.net)
Description: A sandwich ELISA kit for detection of Glutathione Peroxidase 1 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (jsce-ip.com)
Description: A sandwich quantitative ELISA assay kit for detection of Human Actin Alpha 1, Skeletal Muscle (ACTa1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (myelisakit.com)
Description: A sandwich ELISA kit for detection of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (jsce-ip.com)
Here, we set out to characterize the glycocalyx composition of glioblastoma EVs by lectin-binding ELISA and comprehensive immunogold transmission electron microscopy (immuno-TEM). (amsterdamumc.org)
Description: Quantitativesandwich ELISA kit for measuring Human Protein jagged-1 (JAG1) in samples from serum, plasma, tissue homogenates. (kits-elisa.com)
Ligand1
The tyrosine residues of the ITIMs become phosphorylated with the ligand binding, which leads to activation of SHP-1 ( Src homology region 2 domain-containing phosphatase-1), SHP-2 ( Src homology region 2 domain-containing phosphatase-2), SHIP-1 (( Src homology region 2 domain-containing inositol 5′ polyphosphatase-1). (biomedcentral.com)
Specifically binds1
The in vivo grade gemtuzumab biosimilar specifically binds to the human CD33 protein. (sydlabs.com)
Antibody11
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Sialic Acid Binding Ig Like Lectin 10 (SIGLEC10) in serum, plasma, tissue homogenates and other biological fluids. (therabio.org)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Sialic Acid Binding Ig Like Lectin 10 (SIGLEC10) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (master-sistemisanitari-medicinenonconvenzionali.org)
We studied correlates of disease severity in the COMET-ICE clinical trial that randomized 1:1 to placebo or to sotrovimab, a monoclonal antibody for the treatment of SARS-CoV-2 infection. (bvsalud.org)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in Tissue homogenates, cell lysates and other biological fluids. (wildpalm.net)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (wildpalm.net)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Glutathione Peroxidase 1 (GPX1) in serum, plasma and other biological fluids. (jsce-ip.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Glutathione Peroxidase 1 (GPX1) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. (jsce-ip.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) in tissue homogenates, cell lysates and other biological fluids. (jsce-ip.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (jsce-ip.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tankyrase 1 (TNKS1) in Tissue homogenates, cell lysates and other biological fluids. (lipidx.org)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Jagged 1 Protein (JAG1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (kits-elisa.com)
Glycan1
The surface glycan profile of human glioblastoma cell line-derived EVs (50-200 nm) was dominated by α-2,3- and α-2,6 linked sialic acid-capped complex N-glycans and bi-antennary N-glycans. (amsterdamumc.org)
Domain3
It has three parts: (i) V-type Ig domain with sia -binding site, (ii) C1-type Ig domain, (iii) C2-type Ig domain. (biomedcentral.com)
Genetic alterations leading to impaired function of the machinery devoted to clear apoptotic cells, waste nucleic acids and immune complexes play a significant role in both Mendelian and sporadic forms of SLE. (biomedcentral.com)
CD238 is an I-type lectin of approximately 65 kDa, expressed as a monomer on a major subset of NK cells and a subset of CD8+ cells. (bdbiosciences.com)
We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10 6 cells in 100 μL volume]. (elabscience.com)
They are found primarily on the surface of immune cells and are a subset of the I-type lectins. (transcriptionfactor.org)
Interactions2
They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. (wikipedia.org)
The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. (fishersci.com)
Human-specific polymorphic pseudogenization of SIGLEC12 protects against advanced cancer progression, FASEB BioAdvances, 3:69-82, 2021 Jones, Asher, "Rogue" Protein Could Contribute to Humans' High Cancer Rates, The Scientist, April 1, 2021 Maruyama K, Sugano S (1994). (wikipedia.org)
Here, we show that the Omicron RBD binds to human ACE2 with enhanced affinity relative to the Wuhan-Hu-1 RBD and acquires binding to mouse ACE2. (bvsalud.org)
Cell2
B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb. (elabscience.com)
In addition, advanced proteomic analyses have been revealing links between these genetic contributors and complex, malfunctioning signaling pathways (including the upregulation of factors like tumor necrosis factor TNF-α, tumor growth factor TGF-β and interleukin IL-1α) that promote proinflammatory mechanisms via intracellular signaling and trafficking, synaptic function, and cell metabolism/proliferation. (cannabisclinicians.org)
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the "Elabscience Publication Reward Application Form" carefully and send it to [email protected] , we will get back to you with the reward after we confirm it ASAP! (elabscience.com)