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  • tumors
  • BEVERLY, MA-(Marketwired - February 22, 2016) Cellceutix Corporation (OTC: CTIX) (the "Company"), a clinical stage biopharmaceutical company developing innovative therapies with oncology, dermatology, anti-inflammatory and antibiotic applications, is pleased to release the latest p21 data from the recently completed Phase 1 trial of Kevetrin for patients with advanced solid tumors. (ipharminc.com)
  • Now, this new p21 data only reinforces our high expectations for and confidence in Kevetrin, specifically, its potential to treat patients across a broad spectrum of cancer types and even those in late stages with refractory tumors. (ipharminc.com)
  • Overall survival analysis from Q-PCR data obtained from tumors of high risk patients suggests that lower levels of p21 expression could be associated with a poorer outcome. (sigmaaldrich.com)
  • Nutlin-3 also showed good efficacy against tumors with normal MDM2 expression, suggesting that many of the patients with wild-type p53 tumors may benefit from antagonists of the p53-MDM2 interaction. (pnas.org)
  • induces
  • Prolonged p21 up-regulation induces a senescent phenotype in normal and cancer cells, accompanied by an increase in intracellular reactive oxygen species (ROS). (biomedsearch.com)
  • inhibits
  • Follow up work, found evidence that this bistability is underpinned by double negative feedback between p21 and CDK2, where CDK2 inhibits p21 activity via ubiquitin ligase activity. (wikipedia.org)
  • Rest deletion de-represses p21 and inhibits the cardiomyocyte cell cycle and proliferation in embryonic or regenerating hearts. (nature.com)
  • polymerase
  • Polymerase chain reaction based restriction analysis was used for the study of the association of p21 codon 31 polymorphism with bladder cancer. (jove.com)
  • expression
  • The Phase 1 study, conducted at Dana-Farber Cancer Institute and partner Beth Israel Deaconess Medical Center, evaluated not only the safety and pharmacokinetics of Kevetrin, but also assessed changes in p21 expression in peripheral blood monocytes as a measure of p53 activation resulting from Kevetrin treatment. (ipharminc.com)
  • Finally, the suggestion of a dose-response in p21 expression with higher Kevetrin doses will be further evaluated in the planned clinical study in patients with ovarian carcinoma. (ipharminc.com)
  • Overexpression of miR-106b reduced the expression of PTEN and p21 and increased the expression of p-AKT, which is a downstream of PTEN. (biomedcentral.com)
  • Restoring the expression of PTEN or p21 in stably miR-106b-overexpressed cells could rescue the effect of miR-106b on cell radioresistance. (biomedcentral.com)
  • As recent findings show low IFN-β expression by non-responsive monocytes in delayed sepsis patients, we discuss the therapeutic value of blocking or enhancing the levels of this cytokine to modulate immunosuppression. (frontiersin.org)
  • In order to elucidate the significance of p21 expression in lung adenocarcinomas, we performed immunohistochemical analysis of p21, p53 and Ki-67 expression in surgically resected lung adenocarcinomas. (oup.com)
  • All 91 lung adenocarcinomas examined showed p21 immunoreactivity: 39 cases (42.9%) and 52 cases (57.1%) showed high and low p21 expression levels, respectively. (oup.com)
  • More intense p21 expression tended to occur in the more highly differentiated areas. (oup.com)
  • We also further explored the relationship between expression of vimentin and p21, by asking whether vimentin could regulate p21 expression. (sigmaaldrich.com)
  • Conversely, overexpressing vimentin triggered an increase in p21 promoter activity in cells with a nuclear expression of vimentin. (sigmaaldrich.com)
  • Normal cells generally display a rather intense nuclear p21 expression. (novusbio.com)
  • Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). (novusbio.com)
  • B ) Western blot showing the expression of several enzymes involved in glucose metabolism is unaltered in HepG2 cells overexpressing GFP, PTG or GBE1. (bioscirep.org)
  • We found that expression of Batf3 was selectively low in Treg cells but not in effector CD4 (show CD4 ELISA Kits ) T (Teff) cells and that ectopic expression of Batf3 caused a marked reduction in the number of Foxp3 (show FOXP3 ELISA Kits )+ Treg cells. (antibodies-online.com)
  • Data indicte that interferon regulatory factor 8 (IRF8 (show IRF8 ELISA Kits )) expression becomes dependent on transcription factor Batf3 . (antibodies-online.com)
  • These data indicate that HP1-gamma is a novel epigenetic repressor of epsilon-globin (show HBe1 ELISA Kits ) gene expression. (antibodies-online.com)
  • inhibition
  • The inhibition of H2AX with RNA interference or the use of H2AX-deficient cells showed that γH2AX is dispensable for the formation and maintenance of DNA repair foci induced by stalled replication. (asm.org)
  • Clonogenic assays also showed greatest inhibition with this sequence. (aacrjournals.org)
  • It has been shown previously that proteasome inhibition results in the accumulation of HIF-1α in tissue culture under aerobic conditions, although in at least some experimental systems, this is not able to form transcriptionally active HIF-1 heterodimers ( 7 , 8 ). (aacrjournals.org)
  • induction
  • Recent reports show an association of low IFN-β levels with the hyporesponsive state of monocytes from sepsis patients and after endotoxin tolerance induction. (frontiersin.org)
  • contrast
  • In contrast, H2AX-proficient cells as well as H2AX-complemented H2AX −/− cells reacted by increasing p21 levels and arresting the cell cycle. (asm.org)
  • By contrast, REST overexpression in cultured cardiomyocytes represses p21 and increases proliferation. (nature.com)
  • In contrast with the negative cell-cycle regulation, p21 may also serve as an assembly factor for cyclin D-CDK4/6 complexes, thus promoting cyclin D-dependent events, and downstream activation of cyclin E-CDK2 [ 7 , 8 ]. (intechopen.com)
  • In contrast to HP1alpha (show CBX5 ELISA Kits ), HP1gamma could not recognize H3K9me3 in extended nucleosomes. (antibodies-online.com)
  • gene
  • P21 Gene Codon 31 Polymorphism is Associated with Bladder Cancer Urologic Oncology. (jove.com)
  • mitosis
  • Experiments looking at CDK2 activity within single cells have also shown p21 to be responsible for a bifurcation in CDK2 activity following mitosis, cells with high p21 enter a G 0 /quiescent state, whilst those with low p21 continue to proliferate. (wikipedia.org)
  • Uncoupling of S phase and mitosis induced by anticancer agents in cells lacking p21. (biomedsearch.com)
  • Mechanism
  • Our study reveals a REST-p21 regulatory axis as a mechanism for cell cycle progression in cardiomyocytes, which might be exploited therapeutically to enhance cardiac regeneration. (nature.com)
  • By elucidating the REST-p21 genetic mechanism underlying the cell cycle regulation of proliferating cardiomyocytes during cardiac development and regeneration, our study provides an opportunity for developing cell-based therapeutics for heart disease. (nature.com)
  • induce cell
  • These observations illustrated that miR-106b could induce cell radioresistance by directly targeting PTEN and p21, this process was accompanied by tumour-initiating cell capacity enhancement, which is universally confirmed to be associated with radioresistance. (biomedcentral.com)
  • mitotic
  • However, in the absence of H2AX, the AAV-containing cells showed proteosome-dependent degradation of p21, followed by caspase-dependent mitotic catastrophe. (asm.org)
  • anticancer
  • However, there is little direct evidence to show that suppression of NF-κB activation occurs in cancer patients treated with bortezomib, and it is important to consider other potential molecular mechanisms of anticancer effect. (aacrjournals.org)
  • cells
  • Mammary Paget's disease and extramammary Paget's disease are neoplastic conditions, in which there is intraepithelial (usually intraepidermal) infiltration by neoplastic cells showing glandular differentiation. (bmj.com)
  • Working in mouse models, it was also shown that whilst mice lacking p21 were healthy, spontaneous tumours developed and G1 checkpoint control was compromised in cells derived from these mice. (wikipedia.org)
  • Furthermore, H2AX-deficient embryonic stem cells were also genetically unstable, whereas H2AX haploinsufficiency was shown to contribute to genomic instability ( 5 , 13 ). (asm.org)
  • We use several methods to show that human lymphoblastoid cells with mutated MSH6 are unable to mount a robust apoptotic response upon treatment with low doses of alkylator, that mutations in MSH6 impair the proper phosphorylation of threonine 68 on Chk2, and also that p53 Serine 20 phosphorylation (but not Serine 15 phosphorylation) is almost completely abrogated in these mutant cells. (ufl.edu)
  • Mitral cells, the principal cells of the accessory olfactory bulb (AOB), receive monosynaptic input from the sensory periphery and already show highly specific response properties, firing selectively for combinations of genetic markers and gender-specific cues. (jneurosci.org)
  • We have studied dendritic excitability and synaptic integration in AOB mitral cell dendrites, and we show that dendrites of accessory olfactory bulb mitral cells support action potential propagation and can fire regenerative spike-like events that are likely to contribute to the integration of inputs to these cells. (jneurosci.org)
  • Here we show that polymorphonuclear cells (PMNs) serve as key regulators in IL-8-induced HPC mobilization. (jove.com)
  • 0.05 when compared with the corresponding cell line in the presence of glucose ( C ) Western blot showing increased levels of HO-1 in GFP and GBE1 expressing cells, but not PTG expressing cells. (bioscirep.org)
  • D ) Western blot analysis showing increased p21 and decreased p34cdc2 levels in the PPP1R3C knockdown cells. (bioscirep.org)
  • IL-12 (show IL12A ELISA Kits ) production by Batf3 -dependent CD103 + dendritic cells is crucial for maintenance of local Th1 (show HAND1 ELISA Kits ) immunity against L. major infection. (antibodies-online.com)
  • bladder
  • We aimed to investigative the polymorphism of p21 codon 31 as a candidate for the genetic marker of bladder cancer and its progression. (jove.com)
  • 2002
  • Sagittal olfactory bulb slices (300-350 μm thick) were prepared from young mice [postnatal day 14 (P14) to P45, mostly between P21 and Pas described previously ( Urban and Sakmann, 2002 ). (jneurosci.org)
  • dependent
  • Recent work exploring p21 activation in response to DNA damage at a single-cell level have demonstrated that pulsatile p53 activity leads to subsequent pulses of p21, and that the strength of p21 activation is cell cycle phase dependent. (wikipedia.org)
  • proteins
  • On the other hand, γ-H2AX has been shown to be essential for concentrating repair proteins and maintaining the integrity of the DNA damage response foci ( 14 ). (asm.org)
  • Schematic representation of interplay between PCNA, p21 and PCNA-interacting proteins, during NER. (intechopen.com)
  • mice
  • Previously we showed that mobilization could be prevented completely in mice by pretreatment with neutralizing antibodies against the beta2-integrin LFA-1 (CD11a). (jove.com)
  • Here, we show that Cadherin-6 (Cdh6), a homophilic cell adhesion molecule, is a reliable marker of ChCs and Cdh6-CreER mice (both sexes) provide genetic access to hippocampal ChCs (h-ChCs). (jneurosci.org)
  • Batf3 (-/-) mice remained competent for skin graft rejection and delayed hypersensitivity, and retained a CD8 (show CD8A ELISA Kits )(+) dendritic cell population. (antibodies-online.com)
  • PCNA
  • From left to right, are depicted the steps of the binding of PCNA to XPG, followed by the arrival of p21, which then displaces XPG from PCNA, to leave space for binding of the next partner, i.e. (intechopen.com)
  • cell
  • We further show that p21 knockout rescues cardiomyocyte cell cycle and proliferation defects resulting from Rest deletion. (nature.com)
  • targets
  • We further identified PTEN and p21 as novel direct targets of miR-106b by using target prediction algorithms and a luciferase assay. (biomedcentral.com)
  • results
  • Stimate treatment provided opposite results of MO and VMO, showing a decrease in TTO and TTA. (unt.edu)
  • reduction
  • Evidence of vWF involvement in hemostasis and thrombosis was shown using MO and VMO technology to produce a vWD like phenotype, resulting in an increase in TTO and TTA, as well as a reduction in FVIII when blood was tested using the kPTT assay, coinciding with a decrease in vWF. (unt.edu)
  • absence
  • In addition, PrP has been shown to be part of a receptor complex for soluble neurotoxic Alzheimer's disease-associated amyloid-beta peptides [ 15 , 16 ] and the presence or absence of PrP changes NSC self-renewal in response to amyloid-beta peptides [ 17 ], thereby suggesting that a putative neurogenic function of PrP may become corrupted during neurodegenerative disease. (springer.com)
  • occur
  • The involvement of cyanide oxygenase in the metabolism of pyruvate and a-ketoglutarate-cyanohydrin was investigated and shown to occur indirectly by the consumption of free cyanide arising from the cyanohydrins via chemical dissociation. (unt.edu)
  • replication
  • The formation of γ-H2AX foci has also been shown after replication stalling and single-stranded DNA breaks ( 24 , 39 , 55 ). (asm.org)
  • further
  • Further, immunostaining showed that REST was expressed in the majority of cardiomyocytes between embryonic day (E) 11.5 and postnatal day (P) 3, whereas the number of REST-expressing cardiomyocytes was drastically reduced from P3 to P28 (Supplementary Fig. 1c, d ). (nature.com)