Severe dengue antibodies to dengue neutralization tests. Medical search. Frequent questions

In 2010, a special surveillance for West Nile virus (WNV), dengue virus (DENV), and chikungunya virus (CHIKV) was initiated in the Veneto Region of northeastern Italy. (cdc.gov)
Rapid tests included detection of anti-CHIKV IgM by using the OnSite Chikungunya IgM Combo Rapid Test (CTK Biotech, Inc., San Diego, CA, USA) and of DENV nonstructural protein (NS) 1 antigen by using the Dengue NS1 Ag STRIP (Bio-Rad Laboratories, Hercules, CA, USA) on serum samples. (cdc.gov)
Second-line laboratory testing consisted of detection of DENV and CHIKV nucleic acids in plasma specimens by using real-time PCR and endpoint PCR, respectively, and detection of serum IgM and IgG by using an anti-CHIKV indirect immunofluorescence assay (Euroimmun AG, Lübeck, Germany), DENV IgG DxSelect (Focus Diagnostics, Cypress, CA, USA), and DENV IgM Capture DxSelect (Focus Diagnostics). (cdc.gov)
Samples with DENV -positive results by ELISA were further tested by plaque-reduction neutralization test to confirm specificity of antibody response. (cdc.gov)
Sri Lanka is a dengue (DENV) endemic country which since 1988 has introduced vaccination against Japanese encephalitis virus (JEV). (ox.ac.uk)
This cross-reactivity between DENV and JEV is known to affect dengue severity. (ox.ac.uk)
Antibodies to DENV NS1 are associated with severe dengue. (ox.ac.uk)
JEV NS1 responses were higher in older unvaccinated individuals and correlated with higher DENV antibodies. (ox.ac.uk)
The four dengue serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) circulate throughout the Americas, and in some countries simultaneously. (paho.org)
Although dengue virus (DENV) control strategies have targeted vector control and disease surveillance, the development of an effective vaccine is the holy grail of prevention. (blogspot.com)
A candidate vaccine (Sanofi Pasteur, Swiftwater, PA, USA) containing all 4 DENV serotypes is in advanced clinical testing. (blogspot.com)
Although protective immunity was raised in susceptible rhesus monkeys inoculated with all 4 DENVs at a single site, inoculation of 4 chimeric dengue viruses at 1 or 2 sites did not result in neutralizing antibody responses to all 4 DENV ( 3 ). (blogspot.com)
It has therefore been assumed that persons with DENV neutralizing antibodies are protected against infection. (blogspot.com)
In clinical testing of the Sanofi Pasteur vaccine, failure of multiple booster doses to show protection was unexpected because the children were already substantially immune from prior exposure to DENV or Japanese encephalitis vaccine. (blogspot.com)
There is evidence that DENV neutralization titers differ when the same antibodies are assayed in epithelial and Fc-receptor-bearing cells ( 8 ). (blogspot.com)
Some arboviruses, like dengue (DENV), chikungunya (CHIKV) and Zika (ZIKV), have become fully adapted to urban cycles and no longer require NHPs, forest mosquitoes and a sylvatic cycle for their maintenance [ 7 ]. (biomedcentral.com)
While several lines of evidence suggest a protective role of T cells against disease associated with Dengue virus (DENV) infection, their potential contribution to immunopathology in the acute phase of DENV infection remains controversial, and it has been hypothesized that the more severe form of the disease (dengue hemorrhagic fever, DHF) is associated with altered T cell responses. (mdpi.com)
Dengue virus (DENV) is an enveloped, single-stranded RNA virus belonging to the Flaviviridae family. (frontiersin.org)
Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen. (who.int)
Module B contained human serum samples for the detection of anti-DENV antibodies. (who.int)
Among 20 laboratories testing Module A, 17 (85%) correctly detected DENV RNA by reverse transcription polymerase chain reaction (RT-PCR), 18 (90%) correctly determined serotype and 19 (95%) correctly identified CHIKV by RT-PCR. (who.int)
suggested that there are 390 million dengue virus (DENV) infections per year, of which 96 million manifest clinically. (who.int)
Zika virus (ZIKV) and dengue virus (DENV) share a lot of similarities being both phylogenetically closely related, share the same insect vector passage for reaching the host, affinity for the same carbohydrate. (biomedcentral.com)
Developed by scientists at the New York State Department of Health Wadsworth Center and the University of Texas Medical Branch, the assay outperforms single antigen/antibody ZIKV IgM-Capture ELISA testing in overall sensitivity, specificity, positive predictive value and negative predictive value by detecting IgG-IgA-IgM antibodies to Zika Virus (ZIKV) and Dengue virus (DENV) serotypes 1-4. (healthresearch.org)
Unlike DENV, ZIKV infections during pregnancy correlate with severe defects, including microcephaly and neurological disorders. (healthresearch.org)

Dengue and dengue hemorrhagic fever (DHF) result from infection by any of four serotypes of dengue viruses. (cdc.gov)
Health care providers should contact their state or local health department about testing patients with symptoms of Zika virus infection and a compatible travel history. (cdc.gov)
Following infection with one serotype, subsequent infection with a different serotype increases a person's risk of severe dengue and death. (paho.org)
The infection may be asymptomatic, or it may present with symptoms ranging from a moderate fever to a disabling high fever, with severe headache, pain behind the eyes, muscle and joint pain, and rashes. (paho.org)
Studies on human primary immune responses to dengue infection have identified critical attachment sites on the virion for neutralizing antibodies ( 4 ). (blogspot.com)
Myeloid cells are major targets of dengue infection in humans. (blogspot.com)
To our knowledge, only once has an in vitro test correctly predicted which children would be susceptible or have silent infections accompanying a second heterotypic dengue infection ( 9 ). (blogspot.com)
In rare cases, other severe sequelae of coxsackievirus B infection develop, including meningitis and carditis. (medscape.com)
Older people are usually at high risk for severe infection due to underlying health conditions. (johansen.se)
This is because the immune response from the first infection increases the risk of developing DHF upon re-infection, a phenomenon known as antibody-dependent enhancement. (tanzirislambritto.com)
The primary cause of Dengue Hemorrhagic Fever (DHF) is infection by one of the four dengue virus serotypes (DEN-1, DEN-2, DEN-3, and DEN-4). (tanzirislambritto.com)
Interestingly, it's not the first infection with the dengue virus that usually causes DHF but rather a subsequent infection with a different dengue serotype. (tanzirislambritto.com)
In ADE, antibodies from a first dengue infection fail to neutralize a second dengue virus of a different serotype during re-infection. (tanzirislambritto.com)
The primary response of our immune system to an infection is to produce specific antibodies that can neutralize the invading pathogen. (tanzirislambritto.com)
These antibodies will neutralize the virus and aid in its clearance, thus resolving the infection. (tanzirislambritto.com)
The antibodies from the first infection can't neutralize the new serotype effectively. (tanzirislambritto.com)
For instance, infants born to dengue-immune mothers are at a higher risk of severe disease due to the presence of maternal antibodies, which can enhance viral infection. (tanzirislambritto.com)
Pediatric health care providers should work closely with obstetric providers to identify infants whose mothers were potentially infected with Zika virus during pregnancy (based on travel to or residence in an area with Zika virus transmission [ http://wwwnc.cdc.gov/travel/notices ]), and review fetal ultrasounds and maternal testing for Zika virus infection (see Interim Guidelines for Pregnant Women During a Zika Virus Outbreak*) ( 1 ). (blogspot.com)
or 2) infants born to mothers with positive or inconclusive test results for Zika virus infection. (blogspot.com)
The diagnosis of Zika virus infection is made through molecular and serologic testing ( 2 ). (blogspot.com)
When the Zika epidemic spread through the American continent and then later to Africa and Asia in 2015, researchers compared the characteristics of the Zika infection to Dengue, considering both these viruses were transmitted primarily through the same vector, the Aedes aegypti female mosquitoes. (frontiersin.org)
B cells manufacture antibodies which are exquisitely tailored protein tools critical to controlling infection. (uvm.edu)
Alternatively, activated B cells can develop into memory B cells that persist long after infection to provide an institutional memory of those manufactured antibodies to facilitate their rapid remanufacture (or improvement) to protect against a subsequent pathogen encounter. (uvm.edu)
His goal is to develop novel immune metrics and specific antibody tools that can be used to advance vaccine development and to understand host-pathogen interactions during viral infection or vaccination. (uvm.edu)
Our work on dengue and zika immunology draws on an extensive bank of human vaccine clinical trial specimens and natural infection studies in partnership with the Vaccine Testing Center and other collaborators. (uvm.edu)
Dengvaxia is approved for use in individuals 9 through 16 years of age with laboratory-confirmed previous dengue infection and living in endemic areas. (cdc.gov)
Previous dengue infection can be assessed through (a) medical records of a previous laboratory-confirmed dengue infection or (b) serological testing prior to vaccination . (cdc.gov)
We propose that additional investigation into the role of ADCP in protective viral responses, the specific virus epitopes targeted by ADCP antibodies, and the types of phagocytes and Fc receptors involved in ADCP at sites of virus infection will provide insight into strategies to successfully leverage this important immune response for improved antiviral immunity through rational vaccine design. (frontiersin.org)
and monoclonal recombinant antibodies are also currently being pursued for prevention of HIV-1 infection in large Phase IIb clinical trials (NTC02716675 and NCT02568215). (frontiersin.org)
Although the mice initially infected with Chikungunya had low levels of neutralizing antibodies in their bloodstream, these levels rose rapidly after secondary infection with Mayaro, inducing cross-protection against the latter. (azolifesciences.com)
The levels of antibodies produced after each infection were measured. (azolifesciences.com)
Production of memory cells [ B and T lymphocytes ] takes time, but the mice first infected with Chikungunya already had them, so during secondary infection by Mayaro the immune response was faster, including a rise in levels of neutralizing antibodies,' Fumagalli explained. (azolifesciences.com)
This experiment served to demonstrate that antibodies produced in response to infection by Chikungunya also led to cross-protection against Mayaro. (azolifesciences.com)
A specialized blood test, which is exclusively done for the diagnosis of yellow fever, may reveal specific antibodies, which are virus-specific, produced in response to the particular infection. (practo.com)
Dengue virus infection has emerged as marker in clinical practice ( 11 ). (who.int)
An extra step in our assay measures IgG antibodies by avidity testing, which determines the time-frame of infection, whether primary or secondary, and is quick and simple. (healthresearch.org)
Han J, Sun J, Zhang G, Chen H. DCs-based therapies: potential strategies in severe SARS-CoV-2 infection. (medsci.org)
We focus on the essential functions of dendritic cell in severe SARS-CoV-2 infection. (medsci.org)
Thus, it is urgent to develop therapeutic strategies to face the SARS-CoV-2 viral infection at the severe stage. (medsci.org)
On the contrary, second dose of vaccination has been shown to produce enough antibodies against COVID-19 infection, suggestive of its efficiency. (metropolisindia.com)
The disease requires effective post-management of antibody production by complete healers and vaccinators because there is no perfect remedy for the virus infection. (kjcls.org)

After malaria was ruled out, cases were further classified as probable if rapid tests yielded positive results for dengue and chikungunya viruses. (cdc.gov)
West Nile or dengue viruses), so positive results should be confirmed with a more specific test (e.g., plaque-reduction neutralization test). (cdc.gov)
mosquitoes are found throughout much of the Americas, including parts of the United States, and also transmit dengue and chikungunya viruses. (cdc.gov)
The inability of a mixture of 4 dengue chimeric viruses to elicit an initial primary neutralizing antibody response in nonhuman primates and susceptible humans was recognized during preclinical testing and explained by the phenomenon of interference ( 2 ). (blogspot.com)
The introduction of the naturally occurring wMel Wolbachia strain into Aedes aegypti mosquitoes has been shown to reduce the ability of the mosquitoes to transmit dengue and other viruses. (mvcac.org)
It is spread by the yellow fever mosquito ( Aedes aegypti ) and the Asian tiger mosquito ( Aedes albopictus ), which also transmit dengue and chikungunya viruses. (contemporarypediatrics.com)
For dengue, chikungunya, Zika and yellow fever viruses, sylvatic (forest) transmission cycles also exist in some regions and involve non-human primates and forest-dwelling mosquitoes. (biomedcentral.com)
Here we review the investigation methods and available data on sylvatic cycles involving non-human primates and dengue, chikungunya, Zika and yellow fever viruses in Africa, dengue viruses in Asia and yellow fever virus in the Americas. (biomedcentral.com)
mosquitoes are found throughout much of the Region of the Americas, including parts of the United States, and also transmit dengue and chikungunya viruses ( 4 ). (blogspot.com)
Meanwhile, the cross-reactivity of CD8+ and CD4+ T cells response to Dengue and Zika viruses provide important clues for further development of potential treatments. (frontiersin.org)
Together, this information appraises the current understanding of both Zika and Dengue infections, providing insights for future vaccine design approaches against both viruses. (frontiersin.org)
More recently we seek to understand how norovirus, a family of viruses for which there is no vaccine and causes significant gastrointestinal illness (vomiting and diarrhea) and hospitalization can skew the infant antibody response towards those that may not offer protection. (uvm.edu)
Some viruses also can cause more characteristic clinical manifestations, such as severe polyarthralgia or arthritis due to Chikungunya virus or other alphaviruses (e.g. (cdc.gov)
TWiV 1045: Less Lassa, CRISPR RNA viruses September 17, 2023 TWiV reviews a Lassa virus mRNA vaccine that confers protection against disease without inducing neutralizing antibodies, and a CRISPR-based method for engineering the genome of RNA viruses. (microbe.tv)

Other tests can be used such as antibody-capture enzyme-linked immunosorbent assay (ELISA), antigen-capture detection tests, serum neutralization tests, electron microscopy, and virus isolation by cell culture. (unamhe.or.ug)
This allows me to test the exciting hypothesis that a non-HIV antigen may be required to elicit HIV-bnAbs. (wrfseattle.org)
Antiviral activities of antibodies may either be dependent only on interactions between the antibody and cognate antigen, as in binding and neutralization of an infectious virion, or instead may require interactions between antibody-antigen immune complexes and immunoproteins or Fc receptor expressing immune effector cells. (frontiersin.org)
The Fc receptor-dependent function of antibody-dependent cellular phagocytosis (ADCP) provides mechanisms for clearance of virus and virus-infected cells, as well as for stimulation of downstream adaptive immune responses by facilitating antigen presentation, or by stimulating the secretion of inflammatory mediators. (frontiersin.org)
Some functions, such as neutralization, mainly depend on interaction of the Fv domain ( Figure 1A ) with antigen and are therefore predominantly Fc domain independent. (frontiersin.org)
Fc receptor-dependent antibody functions are also involved in activation of downstream adaptive immune responses by facilitating antigen presentation or by stimulating the secretion of inflammatory mediators ( 12 , 13 ). (frontiersin.org)
33 Antigen detection assays are not available for MVEV, but have been used for JEV and Dengue fever virus. (mja.com.au)
Our serologic assay overcomes these barriers by using an antibody-antigen sandwich formed on six Luminex beads with ZIKV envelope, ZIKV NS1, DENV1 NS1, DENV2 NS1, DENV3 NS1 and DENV4 NS1 conjugated to polyvalent IgG+IgA+IgM detector antibodies. (healthresearch.org)
Has a positive test result at the Screening Visit for hepatitis B surface antigen, hepatitis C virus antibody, or HIV type 1 or 2 antibodies. (who.int)
This biopsy material tested positively for La Crosse virus antigen on direct immunofluorescence assay. (medscape.com)
Progress in this arena, however, is being made with a new generation of merozoite antigen targets identified in recent years that exhibit relatively low levels of polymorphism and against which functional neutralizing antibodies can be raised by vaccination. (nature.com)

While the symptoms resembled dengue fever, testing ruled out this and several other potential causes. (wikipedia.org)
It is an illness that affects infants, young children, and adults, with symptoms ranging from mild fever to incapacitating high fever, with severe headache, pain behind the eyes, muscle and joint pain, and rash. (paho.org)
A small number of people (around one per cent) experience severe symptoms, including encephalitis. (johansen.se)
It causes high fever and the typical flu-like symptoms seen in dengue and leads to severe bleeding, blood plasma leakage, and low platelet count. (tanzirislambritto.com)
Clinical symptoms resemble dengue, and while chikungunya is a generally milder disease, debilitating sequelae such as persistent arthralgia have been reported in 36-64% of cases. (who.int)
however, in a significant proportion of infections, individuals develop severe symptoms that can lead to long-lasting lung damage or death. (nature.com)
Our results show that SARS-CoV-2 directs a cGAS-STING mediated, NF-κB-driven inflammatory immune response in human epithelial cells that likely contributes to inflammatory responses seen in patients and could be therapeutically targeted to suppress severe disease symptoms. (nature.com)
28 Indeed, by the time neurological symptoms develop, PCR testing of serum or CSF often returns a negative result. (mja.com.au)
Diagnosis of yellow fever can be a complicated process due to the fact that the symptoms match a variety of other ailments, such as flu , severe malaria , typhoid, leptospirosis, or viral hepatitis. (practo.com)
Monitor your symptoms and do get an RT-PCR test done if you found yourself facing symptoms suggestive of COVID-19 The Delta variant In view of the growing COVID-19 pandemic throughout the world, health officials are evaluating certain coronavirus mutations and variants that could be more contagious or deadly than the original strain. (metropolisindia.com)

Elevations of serum transaminase levels occur in severe hepatorenal disease and might remain elevated for up to 2 months after onset. (cdc.gov)
Laboratory diagnosis generally is accomplished by testing serum to detect virus-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies by serologic assays. (cdc.gov)
We tested archived serum samples of NHPs collected in Kenya for evidence of neutralizing ZIKV antibodies. (cdc.gov)
Serum samples from children given ≥1 doses of the dengue chimeric vaccine can now be tested for primary neutralizing antibody responses to each of the 4 DENVs. (blogspot.com)
Virus-specific IgM antibodies in CSF or serum. (cdc.gov)
Virus-specific IgM antibodies in CSF or serum but with no other testing. (cdc.gov)
2015 we analysed the serum samples of 140 patients with a suspicion of dengue, using ELISA and multiplex polymerase chain reaction. (who.int)
125 countries includ- female mosquitoes, Aedes aegypti and Serum samples that tested positive ing Pakistan ( 2 , 3 ). (who.int)
Has clinical laboratory test results (hematology, serum chemistry, or coagulation) with a toxicity score of Grade = 1 at Screening. (who.int)
Specific immunoglobulin M (IgM) and neutralizing antibodies to the virus in serum or cerebrospinal fluid (CSF) using enzyme-linked immunosorbent assay (ELISA) technique: IgM is usually but not always present during acute illness. (medscape.com)

First, we aimed to increase the detection rate of imported chikungunya and dengue in travelers from areas to which these diseases are endemic, including in new immigrants and settled immigrants visiting relatives and friends, and to promptly identify potential autochthonous cases. (cdc.gov)
It appears Zika's route - from Africa and Asia to Oceania and then the Americas - may mirror that of chikungunya and dengue, both of which are now endemic in a large portion of the Americas. (wikipedia.org)
The second reason dengue should concern health care providers in this country is the potential for encountering cases of dengue among travelers arriving from dengue-endemic areas. (cdc.gov)
all the rest were travelers arriving from dengue-endemic areas. (cdc.gov)
Dengue is endemic to the tropical regions of the world in latitudes from about 35 ° north to 35 ° south. (msdmanuals.com)
An important difference to note, however, was that the Zika epidemic diffused in a shorter time span compared to the persisting feature of Dengue infections, which is endemic in many Asian countries. (frontiersin.org)
The mosquito-borne flaviviruses dengue and zika cause endemic or pandemic disease and long-term sequelae across over 40% of the world's population and there are no broadly licensed vaccines for either. (uvm.edu)

Dengue Plaque Reduction Neutralization Test (PRNT) in Primary and Secondary Dengue Virus Infections: How Alterations in Assay Conditions Impact Performance. (kmptm.pl)
This includes reverse transcription-polymerase chain reaction (RT-PCR) for viral RNA, and immunoglobulin (Ig) M ELISA and plaque reduction neutralization test (PRNT) for Zika virus antibodies. (blogspot.com)
Significant antibody titers include levels of more than 320 by hemagglutination inhibition, more than 128 by complement fixation, more than 256 by immunofluorescence, or more than 160 by plaque reduction neutralization test. (medscape.com)

In May 2023, Oxitec voluntarily withdrew its research authorization application to test the use of genetically engineered mosquitoes in California. (geneconvenevi.org)

The early history of dengue is clouded by the similarity of its clinical picture to that of other febrile illnesses. (cdc.gov)
DHF is marked by the unique clinical features that differentiate it from common dengue fever. (tanzirislambritto.com)
Despite revised clinical classifications of dengue infections by the World Health Organization, the wide spectrum of the manifestations of dengue illness continues to pose challenges in diagnosis and patient management for clinicians. (frontiersin.org)
Clinical disease ranges from mild febrile illness to severe encephalitis. (cdc.gov)
A minority of these cases progress to clinical forms which is with one or more severe complications, such as acute respiratory distress syndrome (ARDS) that is potentially fatal [ 1 , 2 ]. (medsci.org)
This study aimed to develop recombinant proteins specifically responsive to neutralizing antibodies in clinical specimens and use them to develop a rapid diagnostic kit to diagnose neutralizing antibodies quickly and conveniently against the COVID-19 virus and confirm the possibility of commercialization through a performance evaluation. (kjcls.org)

JEV NS1 immunity is probably acquired by cross-reactivity with other flaviviruses such as dengue than through vaccination. (ox.ac.uk)
Consideration of the genetic identity of both the Dengue and Zika flaviviruses as well as the cross-reactivity of relevant T cells along with the actions of CD4+ cytotoxic cells in these infections are also presented. (frontiersin.org)
However, cross-reactive antibodies remain problematic, and may complicate the ability to distinguish between MVEV and other flaviviruses. (mja.com.au)

Therefore, a JEV NS1 ELISA was developed as a community assessment tool for testing seroprevalence. (ox.ac.uk)
An in-house indirect JEV NS1 ELISA was developed and tested in a community cohort of 80 individuals between the ages of 6-80 and with varying JEV vaccine status and past dengue severity. (ox.ac.uk)
The JEV NS1 ELISA is a useful tool to assess seroprevalence in the community and could be potentially useful in community assessment prior to dengue vaccine introduction. (ox.ac.uk)
38 Neutralisation tests or an epitope-blocking ELISA can help identify MVEV-specific antibodies. (mja.com.au)
Les échantillons de sérum de 140 patients susceptibles d'avoir contracté le virus de la dengue ont été analysés entre mai et octobre 2015 à l'aide du test ELISA et de l'amplification en chaîne par polymérase multiplexe. (who.int)
For flavivirus-seropositive group, has positive flavivirus test results (including dengue, West Nile, and Zika) as determined by ELISA or other commercially available serological assay. (who.int)

These results were obtained even though 91% of the children had circulating dengue or Japanese encephalitis antibodies before vaccination, neutralizing antibodies developed to all 4 DENVs, and neutralizing antibody titers increased 2-3 fold after 3 doses of vaccine in 80%-90% of vaccinated children. (blogspot.com)
In the first phase test of the two vaccines, high titers of neutralizing antibodies can be induced, and strong CD4+ and CD8+ reactions are produced, accompanied by mild to moderate adverse reactions. (medicaltrend.org)

It is transmitted by the bite of a mosquito infected with one of the four serotypes of dengue virus. (paho.org)
We determined the frequency of different serotypes of dengue virus to highlight its hyperendemicity in Rawalpindi, Pakistan. (who.int)

Infections with 2 different DENVs can protect against severe disease during subsequent infections ( 6 ). (blogspot.com)
This review discusses similarities between Dengue and Zika infections, comparing their disease transmissions and vectors involved, and both the innate and adaptive immune responses in these infections. (frontiersin.org)
Finally, a summary of the immune biomarkers that have been reported for dengue and Zika viral infections are discussed which may be useful indicators for future anti-viral targets or predictors for disease severity. (frontiersin.org)
Dr. Diehl's work in the Department of Microbiology and Molecular Genetics encompasses translational and basic research aimed at understanding the human immune response to viral infections, primarily to dengue virus, zika virus, norovirus, and SARS-CoV-2. (uvm.edu)

10) Adding to the concern of indigenous dengue transmission is the recent establishment in the United States of another known dengue vector, Aedes albopictus, which was probably imported in shipments of tires from Asia. (cdc.gov)
In the Americas, Aedes aegypti is the mosquito vector that is the main source of dengue transmission. (paho.org)
The Aedes mosquito, mainly Aedes aegypti and, to a lesser extent, Aedes albopictus, plays a crucial role in transmitting the dengue virus, which causes Dengue Hemorrhagic Fever. (tanzirislambritto.com)
Dengue is a disease transmitted by mosquitoes, with the primary vector being the Aedes Aegypti mosquito. (frontiersin.org)
Field trials and modelling studies suggest that elimination of dengue transmission may be possible through widespread release of Aedes aegypti mosquitoes infected with the insect bacterium Wolbachia pipientis (wMel strain), in conjunction with routine dengue control activities. (geneconvenevi.org)

It is important to obtain a yellow fever vaccination history, as IgM antibodies to yellow fever vaccine virus can persist for several years following vaccination. (cdc.gov)
There is currently no specific treatment for dengue, and vector control and vaccination are the only effective methods to prevent future outbreaks. (who.int)
Negative urine pregnancy test at the Screening visit and at the day of each vaccination for females of childbearing potential. (who.int)

Dengue incidence has increased in the Americas over the past four decades, from 1.5 million cumulative cases in the 1980s to 16,2 million in the decade 2010-2019. (paho.org)
more recently, dengue incidence has increased in Central and South America. (msdmanuals.com)
Global dengue incidence has increased in recent decades, though the actual numbers of dengue cases are masked by underreporting. (who.int)

CDC is working with states to expand Zika virus laboratory testing capacity, using existing RT-PCR protocols. (cdc.gov)
West Nile virus is an RNA virus, a close cousin of Dengue , Yellow fever , St. Louis encephalitis and Zika virus , to name a few. (johansen.se)
Further comprehensive diagnostic testing, including testing for Zika virus, should comprise future iterations of the EQA. (who.int)

14,18,19) Nevertheless through the first half of the 20th century, dengue was generally described as a self-limited, nonfatal febrile illness, with occasional hemorrhagic manifestations such as petechiae, epistaxis, gingival bleeding and menorrhagia, that only rarely resulted in more severe or fatal outcomes. (cdc.gov)
We assessed predominantly pediatric patients in Vietnam with dengue and other febrile illness 3 months after acute illness. (researchgate.net)

In 2010, in Veneto Region, Italy, surveillance of summer fevers was conducted to promptly identify autochthonous cases of West Nile fever and increase detection of imported dengue and chikungunya in travelers. (cdc.gov)
People with diabetes and uncontrolled hypertension have a greater risk of developing severe neurological disease from the West Nile virus. (johansen.se)

Hundreds of thousands of cases of dengue and DHF are reported each year in tropical regions of the Americas, Africa, Asia and Oceania. (cdc.gov)
From 1980 through 1987, 879 632 cases of dengue were reported to the Pan American Health Organization from countries in the American region. (cdc.gov)
13-15) From 1980 through 1989, 1457 suspected and 276 confirmed cases of dengue in the continental United States and Hawaii were reported to the Centers for Disease Control. (cdc.gov)
Although the incidence of hemorrhagic dengue in United States travelers abroad is low,(13,15) clinicians in this country may encounter severe cases of dengue, particularly among immigrants from areas with a high incidence of DHF. (cdc.gov)
50-100 million cases of dengue fever brate hosts of dengue virus in nature cessed by multiplex polymerase chain and 500 000 of dengue haemorrhagic are humans and several species of Asian reaction (PCR) in which the presence fever (DHF) reported annually ( 4 ). (who.int)

Since the initial outbreak of the disease, it has been recognized that the sensitivities and specificities for current ZIKV testing algorithms have shortcomings. (healthresearch.org)
This is an important distinction since currently serological assays only use ZIKV envelope or ZIKV NS1 antigens, as well as only one antibody, usually IgM, for their diagnostic assays. (healthresearch.org)

Further validation studies with neutralization assays need to be done on a larger number. (ox.ac.uk)
Twenty-four national-level public health laboratories performed routine diagnostic assays on a proficiency testing panel consisting of two modules. (who.int)
29 , 37 Most reference laboratories have developed in-house MVEV serological tests that can detect total antibody by haemagglutination inhibition, IgG by immunofluorescence assays (IFA) or enzyme immunoassays (EIA), and IgM by IFA or EIA. (mja.com.au)

Of primary concern is the risk of dengue transmission within the continental United States. (cdc.gov)
Approximately 500 million people in the Americas are today at risk of dengue. (paho.org)

Illness caused by Marburg virus begins abruptly, with high fever, severe headache, and severe malaise. (unamhe.or.ug)
Patients who become ill with a fever and severe headache within a few days of a mosquito bite should visit their family physician or any health-care facility. (johansen.se)
Dengue Hemorrhagic Fever (DHF) is an acute and potentially lethal complication of dengue fever, a tropical disease caused by the dengue virus. (tanzirislambritto.com)
What Causes Dengue Hemorrhagic Fever? (tanzirislambritto.com)
Dengue fever usually results in abrupt onset of high fever, headache, myalgias, arthralgias, and generalized lymphadenopathy, followed by a rash that appears with a recurrent fever after an afebrile period. (msdmanuals.com)
Dengue can also cause potentially fatal hemorrhagic fever with a bleeding tendency and shock. (msdmanuals.com)
Treatment is symptomatic and, for dengue hemorrhagic fever, includes meticulously adjusted intravascular volume replacement. (msdmanuals.com)
11 It is also possible that prior immunisation with yellow fever virus or JEV vaccine may induce cross-reactive antibodies. (mja.com.au)
Severe cases of yellow fever can cause high fever, internal haemorrhaging, and liver and kidney damage. (practo.com)

These mosquitoes are highly adaptive to the human environment, coupled with increasing urbanization and inadequate vector control, which has led to a surge in dengue transmission in urban and semi-urban settings. (tanzirislambritto.com)

When a person is infected with the dengue virus for the first time, the immune system responds by producing antibodies specific to that serotype. (tanzirislambritto.com)
However, problems arise when the person is later infected with a different dengue serotype. (tanzirislambritto.com)
DHF and DSS are also influenced by various other factors, such as the strain and serotype of the dengue virus, age, genetic predisposition, and the individual's immune status. (tanzirislambritto.com)

My job is to learn from the patients' samples what is required to induce our immune system to make these super-effective antibodies. (wrfseattle.org)
SARS-CoV-2 mRNA vaccines induce greater complement activation and decreased viremia and Nef antibodies in men with HIV-1. (amedeo.com)
Blood-stage vaccines seek to induce antibodies against the merozoite form of the parasite that invades erythrocytes 2 , and could complement pre-erythrocytic immunity afforded by RTS,S/AS01, protect against disease severity and/or reduce transmission by accelerating the control and clearance of blood-stage parasitemia. (nature.com)

this population is not likely to qualify for access to confirmatory plaque reduction neutralization testing (PRNT) and therefore the virus could go undetected. (healthresearch.org)

17) Hemorrhagic manifestations, including gastrointestinal hemorrhage, were described during dengue epidemics in Texas and Louisiana in 1922. (cdc.gov)

1) Outbreaks of the more severe form of dengue, DHF, occurred in Cuba in 1981 (2) and in Venezuela in 1989. (cdc.gov)
9) Outbreaks of indigenous dengue transmission occurred in Texas in 1980 and again in 1986. (cdc.gov)
Based on information from previous outbreaks, severe disease requiring hospitalization is uncommon and fatalities are rare ( 6 , 7 ). (blogspot.com)
1 Estimated to bear around 70% of the global burden, the Asia Pacific region (comprising the World Health Organization [WHO] South-East Asia and Western Pacific Regions) is an area of high dengue activity with multiple and large outbreaks occurring yearly. (who.int)
In the Western Pacific Region in 2014 alone, there were outbreaks involving 1513 dengue cases in Solomon Islands, 2 45 171 cases in China and 108 698 cases in Malaysia. (who.int)
The causative agent, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), was promptly identified, and determined to be closely related to SARS and the Middle East Respiratory Syndrome (MERS) coronaviruses, which had caused geographically localized outbreaks in 2002-2004 and from 2012 onwards, respectively. (cov19longhaulfoundation.org)
Previously, there were two outbreaks of severe coronavirus caused by different coronaviruses worldwide, namely Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and the Middle East Respiratory Syndrome Coronavirus (MERS-CoV). (medsci.org)

Retrospective Serological Investigation of Severe Acute Respiratory Syndrome Coronavirus Antibodies in Recruits from Mainland China. (kmptm.pl)
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants has challenged the effectiveness of current therapeutic regimens. (biomedcentral.com)
Since the end of 2019, a new type of coronavirus pneumonia (COVID-19) caused by the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been spreading rapidly throughout the world. (medsci.org)
Pneumonia caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is spreading globally. (medsci.org)
In the past dozen years, the outbreak of the novel coronavirus is the third on record spillover of an animal coronavirus to humans after occurring of severe acute respiratory syndrome (SARS) started in 2002 and Middle East respiratory syndrome coronavirus (MERS-CoV) in 2014, which is resulting in a pandemic in global. (medsci.org)

Further work is aimed at identifying novel monoclonal antibodies and T cell responses to the virus. (uvm.edu)
All variants bound the PfRH5 receptor basigin and were recognized by a panel of monoclonal antibodies. (nature.com)

In March 2020 we implemented broad serological testing for SARS-CoV-2, the virus underlying COVID-19 used to profile hospitalized COVID-19 patients as well as those exposed to the virus but did not require hospitalization. (uvm.edu)
20 , 29 - 32 The use of PCR in conjunction with serological tests can increase the sensitivity of WNV diagnostic tests to 94% compared with 45% for serological tests alone. (mja.com.au)
Serological tests remain the mainstay of confirmatory diagnostic testing. (mja.com.au)

In late 2019, a novel severe respiratory illness emerged in Wuhan, China [13] . (cov19longhaulfoundation.org)

We believe that the unanticipated results of the dengue vaccine efficacy trial in Thailand call for new methods of assessing dengue immunity. (blogspot.com)
If the performance of the rapid diagnostic kit is improved and neutralizing antibodies can be analyzed quantitatively using quantitative analysis equipment, it can be used as important data to predict immunity to the COVID-19 virus and determine additional vaccinations. (kjcls.org)

Dengue Branch and the Division of Vector Borne Infectious Diseases, Centers for Disease Control, CID, Fort Collins, CO. Duane J. Gubler, Sc.D and Edward B. Hayes, MD. (cdc.gov)
is the vector mosquito for dengue and is widely distributed in the Americas. (paho.org)
Only Canada and continental Chile are free from dengue and its vector. (paho.org)
To sum up, the cause of DHF is multi-factorial, involving not only the dengue virus and its serotypes but also the human immune response, the vector mosquito species, and various ecological factors. (tanzirislambritto.com)
aegypti) is the major vector that transmits many diseases including dengue, Zika, and filariasis in tropical and subtropical regions. (geneconvenevi.org)

IVI tested international standard candidates in terms of binding antibody titer and neutralizing antibody titer, and transferred the results to the United Kingdom's National Institute for Biological Standards and Control. (ivi.int)
To date, IVI has completed two samples for binding antibody titer and neutralizing antibody titer and the KDCA has since provided four additional samples which are undergoing testing. (ivi.int)

2020 . Case report: dengue and COVID-19 coinfection in Thailand . (ajtmh.org)

Severe disease requiring hospitalization is uncommon and fatalities are rare. (cdc.gov)
Laboratory testing for coronavirus disease (COVID-19) in suspected human cases: interim guidance. (kmptm.pl)
The disease can evolve into severe dengue, characterized by shock, shortness of breath, severe bleeding and / or complications in the organs. (paho.org)
PAHO/WHO provides advice and technical support for dengue prevention and control based on a regional strategy adopted by PAHO / WHO member States in 2016, called the " Strategy for arboviral disease prevention and control " (CD55.R6). (paho.org)
The increasing spread of the dengue virus worldwide and the high fatality rate of DHF make this disease a significant global health concern. (tanzirislambritto.com)
Instead, they aid the virus entering host cells, enhancing its reproduction ability and leading to severe disease. (tanzirislambritto.com)
This leads to increased viral replication, a higher viral load, and a much more severe disease, resulting in DHF or even more severe dengue shock syndrome (DSS). (tanzirislambritto.com)
Довідковий матеріал Dengue is a mosquito-borne disease caused by a flavivirus. (msdmanuals.com)
Dengue is a major public health concern, affecting almost 400 million people worldwide, with about 70% of the global burden of disease in Asia. (frontiersin.org)
As the pathogenesis of viral illnesses is affected by host immune responses, various immune modulators have been proposed as biomarkers to predict the risk of the disease progression to a severe form, at a much earlier stage of the illness. (frontiersin.org)
The purpose of this assignment was to assess our selected article--Isolation of Potent SARS-CoV-2 Neutralizing Antibodies and Protection from Disease in a Small Animal Model--in terms of accuracy, availability, and reliability. (openwetware.org)
Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model. (openwetware.org)
Each disease requires the production of different types of antibody, although some recognize the same proteins. (azolifesciences.com)
SARS-CoV-2 progressed to a global pandemic with substantial consequences due to its high infectivity and transmissibility, and its ability to cause both a severe respiratory illness, and a systemic disease with fatal consequences for vulnerable populations. (cov19longhaulfoundation.org)
Incidence of dengue has increased many fold in the past 50 years and has expanded to areas that were previously free from the disease. (who.int)
In addition, it was reported that elderly patients with SARS-CoV-2 are at significant risk to develop severe disease and that the proportion of severe cases is elevated in hypertensive or diabetic patients with SARS-CoV-2 [ 7 ]. (medsci.org)
Only a few state laboratories or other specialized laboratories, including those at the Centers for Disease Control and Prevention (CDC), are capable of performing this specialized testing. (medscape.com)
Plasmodium falciparum parasites are the causative agent of the most severe form of human malaria, and the development of an effective vaccine remains a key strategic goal to aid the control, local elimination and eventual eradication of this disease. (nature.com)

Many patients have an uneventful recovery, but in approximately 15% of infected persons, the illness recurs in more severe form within 48 hours following the viremic period. (cdc.gov)
The illness can evolve to severe dengue, characterized by shock, respiratory distress, severe bleeding, and/or serious organ impairment. (paho.org)
There are several reasons to be worried about, including faster transmission, more severe illness, decreased neutralisation by antibodies and or reduced efficiency of treatment and vaccines.The pandemic is far from over. (metropolisindia.com)

When the tetravalent dengue chimeric vaccine was given to partially dengue-immune children and adults in the Philippines, a broad neutralizing antibody response was observed after administration of only 2 vaccine doses ( 7 ). (blogspot.com)
Detailed studies on innate immune responses in human myeloid cells with a variety of dengue immune complexes should proceed forthwith. (blogspot.com)
This knowledge is critical to developing better vaccines and to develop better laboratory tests to determine whether vaccine candidates engage the immune system in a way that is likely to lead to protection. (uvm.edu)
These Fc receptor-dependent antibody functions provide a direct link between the innate and adaptive immune systems by combining the potent antiviral activity of innate effector cells with the diversity and specificity of the adaptive humoral response. (frontiersin.org)
Antibodies are a key component of the human adaptive immune system, and the elicitation of antibodies has been correlated with vaccine efficacy in many diseases ( 1 ). (frontiersin.org)
Fc receptor-dependent antibody functions are important components of the immune response that provide mechanisms for clearance of infected host cells, immune complexes, or opsonized pathogens. (frontiersin.org)
The researchers analyzed neutralization by antibodies but also identified other factors in the mice's immune system that could influence this cross-protection. (azolifesciences.com)
The immune response to pathogens is both innate [ macrophages, neutrophils, natural killer cells ] and adaptive [ B and T lymphocytes ], as well as involving soluble mediators such as antibodies and cytokines. (azolifesciences.com)
Therapies aimed at modulating immune activation to attenuate the detrimental inflammatory response or promote an antiviral cytokine response represent an important avenue for treating patients with severe COVID-19. (nature.com)

This work also directly led to the development of a new antibody (Nirsevimab) that can protect premature infants after one dose for an entire winter season against respiratory syncytial virus (RSV). (uvm.edu)

d WHO Collaborating Centre for Arbovirus Reference and Research (Dengue/Severe Dengue), Tropical Infectious Diseases Research and Education Centre, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. (who.int)
In other words, Mayaro and Chikungunya trigger the production of different antibodies, but some of these antibodies are effective against both diseases,' Fumagalli said. (azolifesciences.com)
ABSTRACT Dengue is one of the most important arthropod-borne viral diseases. (who.int)
Dengue virus is transmitted to was approved by the Ethics Committee borne viral diseases worldwide ( 1 ). (who.int)

He studied how naïve B cells that do not yet make antibodies, become activated (in part by CD4+ T cells) to scale up and develop into antibody-producing cellular factories such as plasmablasts and plasma cells. (uvm.edu)
When a vaccine is administered to an individual, they can make antibodies to many different parts of the virus. (metropolisindia.com)

SARS-CoV-2 specific antibody responses in COVID-19 patients. (kmptm.pl)
The effect of JEV NS1 on dengue severity has not been evaluated in Sri Lanka and underlying JEV NS1 seroprevalence can potentially be important in dengue vaccine responses. (ox.ac.uk)
We and others have described the unique biologic responses when dengue virus-antibody complexes are presented to myeloid cells ( 8 ). (blogspot.com)
These severe cases are often associated with high levels of pro-inflammatory cytokines and low antiviral responses, which can cause systemic complications. (nature.com)
The antibodies induced by one protein variant were shown to be qualitatively similar to responses induced by other vaccine platforms. (nature.com)

Diagnosis involves serologic testing and polymerase chain reaction (PCR). (msdmanuals.com)
Polymerase chain reaction (PCR) for the diagnosis of La Crosse encephalitis and Metagenomic next-generation sequencing (NGS) of the CSF has the potential to identify a broad range of pathogens in a single test (ref DOI: 10.1056/NEJMoa1803396). (medscape.com)

Moreover, the Delta plus variant of coronavirus, due to its mutation of K417N spike protein, may escape vaccines and antibodies more than the Delta variant. (metropolisindia.com)

Book a quantitative COVID antibody test and check how many antibodies you developed after completing your COVID jabs. (metropolisindia.com)
Analysis following immunization of rabbits identified quantitative and qualitative differences in terms of the functional IgG antibody response against the P. falciparum parasite. (nature.com)

Dengue prevention and control must be intersectoral and involve the family and the community. (paho.org)

46 There are numerous reports of immunotherapy for WNV encephalitis in Israel and the United States with WNV-specific intravenous immunoglobulin (Omr-IgG-am, Omrix Biopharmaceuticals, Tel Aviv, Israel) obtained from donors with high titres of WNV antibodies. (mja.com.au)
Left image of a CT scan of an 8-year-old boy with severe La Crosse encephalitis complicated by uncal herniation (obtained on the second hospital day) reveals brain edema with associated obliteration of perimesencephalic cisterns (arrows). (medscape.com)
On the right, a T2-weighted magnetic resonance image obtained from a 7-year-old boy with severe La Crosse encephalitis shows focal areas of increased signal intensity in the right temporoparietal and left frontotemporal regions (arrows). (medscape.com)
Brain biopsy specimen from a 7-year-old boy with severe La Crosse encephalitis (hematoxylin and eosin stain, 200X). (medscape.com)

One of the earliest, if not the first, dengue epidemics described in the medical literature occurred in Philadelphia in 1780. (cdc.gov)
5-7) Dengue epidemics were reported in various parts of the south- eastern United States through the 19th and 20th centuries, (6) most recently in Louisiana in 1945. (cdc.gov)
Dengue-like epidemics occurred in Egypt and on Java in 1779, but these may actually have been caused by chikungunya virus. (cdc.gov)
7) Dengue or dengue-like epidemics were reported through- out the 19th and early 20th centuries in the Americas, southern Europe, North Africa, the Middle East, Asia and Australia and on various islands in the Indian Ocean, South and Central Pacific and the Caribbean. (cdc.gov)
7) Deaths occurred during dengue epidemics in Australia in 1897 and in Greece in 1928, when over 1000 deaths were reported. (cdc.gov)

Samples from persons with possible cases were sent to the regional reference laboratory for second-line laboratory testing and confirmation. (cdc.gov)
As stated by Kuno & Chang [ 15 ], "The three commonly used data for identifying vertebrate reservoirs for arboviruses have been (i) virus isolation from suspected animals, (ii) relatively high antibody prevalence in the animals captured in the field and (iii) demonstration of viraemia (of higher virus titre and duration) in the suspected animals typically obtained under laboratory conditions" [ 15 ]. (biomedcentral.com)
Accurate laboratory testing is a critical component of dengue and chikungunya surveillance and control. (who.int)

Healthcare providers should contact their state or local health department and CDC (at telephone 1-970-221-6400) for assistance with diagnostic testing. (cdc.gov)
I built tools to study this virus and contributed to a diagnostic test during the first year of my M.Sc. (wrfseattle.org)

Severe haemorrhagic manifestations may appear between five and seven days from symptom onset, and fatal cases usually have some form of bleeding, often from multiple areas. (unamhe.or.ug)

It develops faster than dengue , malaria , or chikungunya . (practo.com)

Anatomy2

Organisms25

Diseases11

Chemicals and Drugs18

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes10

Information Science2

Health Care6

Geographicals4

DENV25

Infection41

Viruses14

Antigen11

Symptoms10

Serum10

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20231

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ELISA6

Titers2

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Infections4

Aedes5

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Incidence has increased3

Zika virus3

Febrile illness2

West Nile2

Cases of dengue5

ZIKV2

Assays3

Risk of dengue2

Fever9

Mosquitoes1

Serotype3

Induce3

PRNT1

Hemorrhagic1

Outbreaks7

Acute5

Monoclonal antibodies2

Serological3

20191

Immunity2

Vector5

Titer2

20201

Disease18

Illness3

Immune9

Respiratory1

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Make antibodies2

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Polymerase chain re2

Protein1

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Malaria1