Code for cardio scRNA-seq analysis and figure plots from manuscript A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes . (github.com)
Manuscript data set contains single cell RNA-sequencing data from in vitro hiPSC derived cardiomyocytes and includes both raw (fastq) and processed data (count matrix). (github.com)
Confocal immunofluorescence image of hiPSC-derived cardiomyocytes stained for the sarcomere proteins cardiac troponin T (red) and a -actinin (green). (tum.de)
We compared this data with existing murine and 3D cardiac organoid scRNA-seq data and confirmed the dominance of left ventricular cardiomyocytes (>90%) in our hiPSC-derived progeny. (stanford.edu)
Fibroblast1
Here, we describe the StemCellFactory, an automated, modular platform covering the entire process of hiPSC production, ranging from adult human fibroblast expansion, Sendai virus-based reprogramming to automated isolation, and parallel expansion of hiPSC clones. (frontiersin.org)
Differentiation4
We attempted to establish a hiPSC line that stably expresses dCas9-VPR after differentiation into neurons. (jax.org)
It is evident that the dCas9-VPR transgene inserts randomly into the genome since the success rate of transgenic hiPSC to neuron differentiation is quite low. (jax.org)
The differentiation method presented here generates hiPSC-ECs in only 6 days, without addition of animal sera and at high efficiency, hence providing a scalable source of hiPSC-ECs. (biomedcentral.com)
Thus, the unmatched demand is a robust hiPSC-EC differentiation protocol, which is highly reproducible, cost-effective, omits animal-derived media compounds and yields highly pure ECs. (biomedcentral.com)
Neurons2
Preliminary analysis suggests that combinations of small molecules, such as EB1089 + Selenite + R08191/IFNARIN are the most effective in increasing stroke resiliency in hiPSC derived neurons. (jax.org)
Although the vast majority of cell clones dies, we successfully differentiated a clone into cortical neurons and characterized the effectiveness of the technology to integrate into the genome. (jax.org)
Donor1
By the end of year three, we have generated iPSC clones from 1737 donor samples (out of 2670 received). (ca.gov)
HiPSCs5
While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
Our set-up enables automated, user-independent expansion of hiPSCs under fully defined conditions, and could be exploited to generate a large number of hiPSC lines for disease modeling, and drug screening at industrial scale, and quality. (frontiersin.org)
However, to realize the full potential of hiPSCs for disease modeling and drug screening, several challenges still need to be overcome, as there is the high variability between hiPSC lines, the risk of accumulating genetic aberrations when culturing hiPSCs, and the lack of standardized procedures for hiPSC generation as such (reviewed by Volpato and Webber, 2020 ). (frontiersin.org)
This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs. (addgene.org)
Vector3
The plasmid map, partial sequence will be disclosed upon purchase, so that you can construct a cloning vector, though we are not disclosing the landing pad sequences, nor the entire plasmid sequence. (appliedstemcell.com)
To create plasmid that expresses zebrafish leukemia inhibitory aspect (Lif) the zebrafish cDNA [16] was sub-cloned right into a vector that transported the puromycin resistant gene (and Rev2: had been utilized to amplify cDNA that encodes zebrafish Gdnf from zebrafish testicular cDNA using Benefit? (bio-biz-navi.com)
The ensuing PCR product was initially cloned into pGEM-T-easy vector (Promega Madison WI USA) and sub-cloned into pZeoSV2 (Invitrogen) to create pZeoSV2-Gdnf plasmid. (bio-biz-navi.com)
Human3
hiPSC (human induced pluripotent stem cell) are a source of potential cellular immunotherapies. (aicompanies.com)
We routinely produce engineered heart tissue based on hiPSC-derived cardiac cells seeded on decellularized ventricular matrix from pigs or non-human primates, which we cultivate in a biomimetic chamber system promoting tissue maturation by continuous mechanical and electrical stimulation. (tum.de)
The CReM iPSC Core is offering basic training in human induced pluripotent stem cell (hiPSC) culture for research investigators working with or planning to work with hiPS cells. (bu.edu)
Banks1
The easy access to blood samples using the new technique could potentially boost the recruitment of greater numbers and diversities of donors, and could lead to the establishment of large-scale hiPSC banks. (stonescryout.org)
Therapeutic2
Once a therapeutic target has been identified, we also use hiPSC-derived cells as a platform for testing novel therapies, as we have previously done for Duchenne muscular dystrophy (DMD). (tum.de)
hiPSC Modeling of Lineage-Specific Smooth Muscle Cell Defects Caused by TGFBR1(A230T) Variant, and Its Therapeutic Implications for Loeys-Dietz Syndrome. (nih.gov)
Establish1
Infection of iPSCs with lentiviral inserts is a highly efficient process since stem cells grow quickly, remain undifferentiated in specific cell culture conditions and can establish fully infected clones within 2-3 passages. (nature.com)
Puromycin1
Clones were selected due to their puromycin resistence. (hpscreg.eu)
Lines2
It would be beneficial to continue to study small molecule treatments across various hiPSC-derived cortical neuron lines, as well as dCas-9VPR technology that transfects into a safe harbor locus in the genome to ensure expression. (jax.org)
Serve as a repository for sharing control and disease-specific hiPSC lines with internal and external investigators to conduct both basic and translational research. (bu.edu)
Disease2
In particular, hiPSC-based models provide a powerful tool to identify genetic risk factors, and to study cellular and molecular mechanisms that contribute to the pathogenesis of a disease. (frontiersin.org)
A major focus of our research is using hiPSC-based disease modelling to gain insights into the molecular and cellular mechanisms involved in cardiovascular disorders such as arrhythmogenic right ventricular cardiomyopathy (ARVC), hypoplastic left heart syndrome (HLHS), cardiomyopathy associated with Noonan syndrome, left ventricular non-compaction (LVNC), and others. (tum.de)
Majority1
Furthermore, the majority of hiPSC-ECs express the vascular endothelial marker CD184 (CXCR4). (biomedcentral.com)
Control1
Panel A. DEF-hiPSC ChiPSC18 cell�� CD81 negative( �� ) control positive( �� ��) control. (takara.co.kr)
Combination1
This combination generates hiPSC derived ECs (hiPSC-ECs) at a fraction of 90.9 ± 1.5% and is easily transferable from the two-dimensional (2D) monolayer into three-dimensional (3D) scalable bioreactor suspension cultures. (biomedcentral.com)
Positive1
hiPSC-ECs are positive for CD31, VE-Cadherin, von Willebrand factor and CD34. (biomedcentral.com)
IPSC1
Resultant iPSC clones are validated for pluripotency, authenticity, and genetic integrity. (reprocell.com)
HESC1
hiPSC clones exhibiting characteristic human embryonic stem cell (hESC) morphology were isolated ~45 days after transduction ( Supplementary Fig. 1a ). (nature.com)
Tissue1
Cardiac tissue comprised of RyR2-H29D hiPSC-CMs exhibited aberrant contractile properties that were largely prevented by S107, flecainide and propranolol. (bvsalud.org)
Partial1
CONCLUSIONS: By testing 4 drugs on patient-specific PMVT hiPSC-CMs, we concluded that S107 and flecainide are the most potent molecules in terms of preventing the abnormal SR Ca2+ release and contractile properties in RyR2-H29D hiPSC-CMs, whereas the effect of propranolol is partial, and verapamil appears ineffective. (bvsalud.org)
Line1
hiPSC line YZWJs524 (Kyoto University's Center for iPS Cell Research and Application). (researchsquare.com)
Cells1
This study validated our published hiPSC array-based system as a useful platform for GWAS and confirmed the role of NDUFA4 as a susceptibility locus for ZIKV in disease-relevant trophectoderm cells. (bvsalud.org)
Single1
We isolated and characterized single cell clones. (nih.gov)
Order2
Chromosomal integrity is the first quality control the core facility applies to hiPSC generated clones in order to identify the ones that will be selected for the rest of the workflow. (stemgenomics.com)
In the present study, we evaluated the effects of clinical and experimental anti-arrhythmic drugs on the intracellular Ca2+ handling, contractile and molecular properties in PMVT hiPSC-CMs in order to model a personalized medicine approach in vitro. (bvsalud.org)
Quality control1
quality control of targeted clones does. (nih.gov)
System1
2. Efficient generation of hiPSC neural lineage specific knockin reporters using the CRISPR/Cas9 and Cas9 double nickase system. (nih.gov)
Similar1
Thus, the hiPSC-derived EpSCs are folliculogenic and functionally similar to bulge EpSCs. (nature.com)
Technologies1
Using patient-specific hiPSC and CRISPR/Cas9 technologies, we showed that S107 is the most efficient in vitro candidate for treating the short-coupled PMVT at rest. (bvsalud.org)
Learn1
Learn about a new, improved hiPSC-derived beta-cell model that addresses the shortcomings of current models. (takarabio.com)
Channel1
RESULTS: The voltage-dependent Ca2+ channel inhibitor verapamil did not prevent the aberrant release of sarcoplasmic reticulum (SR) Ca2+ in the RyR2-H29D hiPSC-CMs, whereas it was prevented by S107, flecainide or propranolol. (bvsalud.org)