Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
Dovitinib enhanced the BMP-2-induced alkaline phosphatase (ALP) induction, which is a representative marker of osteoblast differentiation. (molcells.org)
they not only effectively induced macrophage polarization to M2 phenotype but also promoted the migration, tube formation, and angiogenic differentiation of human umbilical vein endothelial cells (HUVECs) and facilitated the migration, osteo-differentiation, and extracellular matrix (ECM) mineralization of MC3T3-E1 cells. (biomedcentral.com)
For the quantification of cellular differentiation at the molecular level, osteogenic differentiation of MSCs is controlled by the interaction of hormones and transcription factors: runt-related transcription factor-2 (RUNX2) effectuates the expression of bone-specific genes, e.g. osterix (OSX), collagen type 1 alpha-1 (COL1A1), osteocalcin (OC), and bone sialoprotein (BSP) by binding to the promoters of these genes. (biomedcentral.com)
Generally, alkaline phosphatase (ALP), COL1A1, BSP, RUNX2, transforming growth factor-beta 1 (TGFB1), osteonectin (ON), and bone morphogenetic protein-2 (BMP2) are known to be early markers of osteoblastic differentiation, whereas OC and osteopontin (OPN) are expressed later in the differentiation process [ 20 ]. (biomedcentral.com)
Syndrome1
Association of Neuropeptide Y Gene rs16147 Polymorphism with Cardiovascular Risk Factors, Adipokines, and Metabolic Syndrome in Patients with Obesity. (cdc.gov)
Variety of metabolic2
We have conducted large population studies relating serumosteocalcin levels to race, gender, normal growth, development, and aging and to a variety of metabolic bone diseases including osteoporosis. (yale.edu)
High leptin levels are interpreted by the brain that energy reserves are high, whereas low leptin levels indicate that energy reserves are low, in the process adapting the organism to starvation through a variety of metabolic, endocrine, neurobiochemical, and behavioral changes. (cloudfront.net)
Proteins3
Current in vivo studies include analysis of the phenotype of several transgenic mice with targeted deletions of bone matrix proteins. (yale.edu)
even in all healthy humans tested, a significant percentage of the extra-hepatic VKD proteins, which are activated by vitamin K2, e.g., osteocalcin and matrix Gla-protein, are only partly carboxylated and therefore inactive. (vitamindwiki.com)
We are (1) investigating the source and regulation of osteocalcin production, and (2) identifying regulators of osteocalcin synthesis and secretion in vivo and in vitro. (yale.edu)
Respectively, in vitro, freezing and thawing serum samples may decrease the binding of calcium as well. (abdominalkey.com)
Osteoporosis2
It has been observed that higher serumosteocalcin levels are relatively well correlated with increases in bone mineral density during treatment with anabolic bone formation drugs for osteoporosis, such as teriparatide. (wikipedia.org)
My laboratory is interested in the development of serum markers for the evaluation of osteoporosis and metabolic bone disease. (yale.edu)
Secretion2
We are currently actively involved in characterizing the circulating forms of osteocalcin and defining the factors that influence its synthesis and secretion. (yale.edu)
Osteocalcin promotes pancreatic β-cell proliferation and insulin secretion, and also affects the insulin sensitivity of peripheral tissues. (dntb.gov.ua)
Biosynthesis2
In the testes, osteocalcin acts on Leydig cells, stimulating testosterone biosynthesis and therefore affects male fertility. (wikipedia.org)
Purpose: Undercarboxylated-Osteocalcin (ucOCN), acting on its putative receptor GPRC6A, was shown to stimulate of testosterone (T) production by Leydig cells in rodents, in parallel with the hypothalamus-pituitary-gonadal axis (HPG) mediated by luteinizing hormone (LH). (endocrine-abstracts.org)
Osteoblasts2
Osteocalcin is secreted solely by osteoblasts and thought to play a role in the body's metabolic regulation. (wikipedia.org)
Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a small (49-amino-acid) noncollagenous protein hormone found in bone and dentin, first identified as a calcium-binding protein. (wikipedia.org)
We have focused primarily on osteocalcin, a vitamin K-dependent protein synthesized only in bone. (yale.edu)
Serum insulin-like growth factor (IGF)-I and IGF-binding protein-3 concentrations and prostate cancer risk: results from the European Prospective Investigation into Cancer and Nutrition. (mdc-berlin.de)
An increase in serum albumin concentration of 1 g/dL increases protein-bound calcium by 0.8 mg/dL, whereas an increase of 1 g/dL of globulin increases protein-bound calcium by 0.16 mg/dL. (abdominalkey.com)
To that end, we have developed site specific antibodies and are investigating the circulating forms of osteocalcin through the use of these monoclonal antibodies. (yale.edu)
Calcium13
Impairment of the capability of the kidney to produce and reuptake enough calcitriol and therefore maintain vitamin D, phosphate, and calciumhomeostasis is one of the principal pathophysiological components of metabolic bone disease in CKD. (karger.com)
The normal range for total serumcalcium must be established for each laboratory and varies according to the method used. (abdominalkey.com)
Ionized calcium in normal serum, ultrafiltrates and whole blood determined by ion-exchange electrode. (abdominalkey.com)
Thus, it is obvious that changes in total serumcalcium concentration cannot be used for the assessment of the effect on bound calcium concentration unless the changes in albumin and globulin concentrations also are determined. (abdominalkey.com)
Serum ultrafiltrable calcium is obtained by applying pressure on serum against a semipermeable membrane. (abdominalkey.com)
Thus, serum water is forced across the membrane, and the ultrafiltrate is analyzed for calcium concentration and then corrected for total serum solids. (abdominalkey.com)
Determinations are best made on freshly separated serum, because heparin creates complexes with calcium, and the presence of fibrin may interfere with the structural integrity of the porous membrane used in the procedure. (abdominalkey.com)
An increase in serum pH of 0.l unit may cause a decrease in ionized calcium of 0.16 mg/dL ( 1 , 2 ). (abdominalkey.com)
The amount of complexed calcium is measured indirectly by subtracting the ionized calcium (47%) from the ultrafiltrable calcium (60%) and thus equals about 13% of total serumcalcium. (abdominalkey.com)
In the pancreas, osteocalcin acts on beta cells, causing beta cells in the pancreas to release more insulin. (wikipedia.org)
In fat cells, osteocalcin triggers the release of the hormone adiponectin, which increases sensitivity to insulin. (wikipedia.org)
Alkaline2
The behavior of mesenchymal stem cells from an umbilical cord vein was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a mineralization assay, and an alkaline phosphatase activity assay and by carrying out a quantitative real-time polymerase chain reaction for osteogenic markers. (biomedcentral.com)
The laser-processed titanium surface increased the proliferation of CHO-k1 cells, reduced the proliferation of mesenchymal stem cells, upregulated the expression of the osteogenic markers, and enhanced alkaline phosphatase activity. (biomedcentral.com)
The Effect of Genistein Supplementation on Vitamin D Levels and Bone Turnover Markers during the Summer in Healthy Postmenopausal Women: Role of Genotypes of Isoflavone Metabolism. (cdc.gov)
Kaygusuz SB, Alavanda C, Kirkgoz T, Eltan M, Yavas Abali Z, Helvacioglu D, Guran T, Ata P, Bereket A, Turan S. Does Genotype-Phenotype Correlation Exist in Vitamin D-Dependent Rickets Type IA: Report of 13 New Cases and Review of the Literature. (abdullahbereket.com)
Illnesses2
Renewing interest in the study of intermediate metabolism and cellular bioenergetics is brought on by the global increase in the prevalence of metabolic illnesses. (mdpi.com)
Many epidemiological studies show that cardiovascular illnesses, heart stroke and metabolic symptoms are connected with abnormal degrees of liver organ enzymes, such as for example AST and ALT [31, 32]. (techieindex.net)
Pathogenic1
In the advanced stages of the disease, the pathogenic bacteria of dental plaque and their metabolic products may enter into the systemic circulation during mastication or mechanical procedures. (encyclopedia.pub)
Associations of SerumOsteocalcin and Polymorphisms of the Osteocalcin Gene with Bone Mineral Density in Postmenopausal and Elderly Chinese Women. (cdc.gov)
Cells1
However, whether RESV promotes function of senescent stem cells in alleviating age-related phenotypes remains largely unknown. (thno.org)
Factors1
That is accomplished by transferring factors that modulate different metabolic and signaling pathways. (frontiersin.org)
Plays an important role2
In the brain, osteocalcin plays an important role in development and functioning including spatial learning and memory. (wikipedia.org)
It is shown that the level of osteocalcin in the blood serum is significantly reduced compared to healthy controls, both in patients with type 1 diabetes mellitus and, especially, in type 2 diabetes mellitus. (dntb.gov.ua)
Given the importance of developing new approaches to the diagnosis and correction of metabolic disorders in diabetic patients, the study of the influence of bone hormones on hormonal and metabolic parameters and bone status, including the risk of fractures, remains relevant in modern diabetology. (dntb.gov.ua)
Muscle1
In muscle, osteocalcin acts on myocytes to promote energy availability and utilization and in this manner favors exercise capacity. (wikipedia.org)
Studies2
In many studies, osteocalcin is used as a preliminary biomarker on the effectiveness of a given drug on bone formation. (wikipedia.org)
Recent studies have demonstrated the importance of bone as an endocrine organ that produces biologically active substances, which regulate both local bone metabolism and metabolic functions throughout the body. (dntb.gov.ua)
Levels1
Injections of high levels of osteocalcin alone can trigger an ASR in the presence of adrenal insufficiency. (wikipedia.org)
Liver1
nonalcoholic fatty liver organ disease (NAFLD) is known as an element of metabolic symptoms, which is connected with atherosclerosis highly, coronary disease and heart stroke [32C34]. (techieindex.net)
Formation2
For instance, one study which aimed to study the effectiveness of a glycoprotein called lactoferrin on bone formation used osteocalcin as a measure of osteoblast activity. (wikipedia.org)
HSI: hepatic steatosis index, non-HDL-C: serum degree of non-HDL cholesterol CW069 Debate Main results and interpretation The prevalence of scleroedema in sufferers with diabetes mellitus (9.7%) identified within this research was like the beliefs reported in previous observational research (2.5C14%) [16, 17]. (techieindex.net)
Several reports demonstrated even more frequent presence from the aPLs in the sera of sufferers with type II diabetes mellitus. (techieindex.net)
Development1
Although, the disease appears as a locoregional infection, the periodontal pathogens, in addition their metabolic products and systemic mediators, receive access to the bloodstream, thereby contributing to the development of systemic disorders. (encyclopedia.pub)
Activity1
Metabolic statuses of BMMSCs and the mitochondrial activity, transcription and morphology were also examined. (thno.org)