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Coxiella burnetiiCoxiellaQ FeverGoat DiseasesAntibodies, BacterialGoatsSheep DiseasesEndocarditis, BacterialDNA, BacterialZoonosesComplement Fixation TestsSeroepidemiologic StudiesCOS CellsRickettsiaAbortion, VeterinaryVacuolesRickettsia rickettsiiVeterinariansPefloxacinDoxycyclineHeart ValvesSerumBartonella quintanaDairyingTicksMilkLivestockPolymerase Chain ReactionCattle DiseasesBartonellaChlamydophilaAntigens, BacterialBlotting, SouthwesternBacterial VaccinesEnvironmental MicrobiologyFluorescent Antibody Technique, IndirectCortisone ReductaseSerologic TestsBacterial Secretion SystemsTransfer AgreementBiological Specimen BanksWaste ManagementTissue BanksSpecimen HandlingCase-Control StudiesTime FactorsBhopal Accidental ReleaseEncyclopedias as TopicChemical Hazard ReleaseAustraliaDissent and DisputesDrug IndustryFederal Government
AntibodiesBacteriumInfectionSerologicalELISASpecimensSeroprevalenceGenotyping of CoxiellaDetectionZoonosis causedRickettsiaObligate intracellularExposureSpecimenAcute Q feverMolecularAgent of Q feverRuminantsAntibodyAerosolsBurnettiSerologicPathogenInfectious DiseasePhaseMilk samplesBacteriaGoatGoatsChronic Q-feverSeropositiveUrineDistinguishVeterinaryBovineEndocarditisRickettsialVaccineSamplesPlasmaOccurrenceGenotypesLiver enzymesPresence2018NHANESEpidemiologic
Antibodies12
  • Their susceptibility to C. burnetii infection has been confirmed by the presence of serum antibodies ( 2 ), but strong evidence for pigs serving as reservoirs of C. burnetii is lacking. (cdc.gov)
  • Sera from patients and the control group were subjected to serological tests to search specific antibodies to the bacteria. (medscimonit.com)
  • Detection of antibodies to Coxiella burnetii, the causative agent of Q fever, by ELISA. (tamu.edu)
  • The IDEXX Q Fever Ab Test is an enzyme immunoassay for the detection of antibodies against Coxiella burnetii in serum, plasma and milk samples of ruminants. (idexx.com)
  • Use of ELISA testing allows for the serological diagnosis of Q fever by detecting C. burnetii -specific antibodies. (idexx.com)
  • Background SERION ELISA classic Coxiella burnetii tests are recommended for the detection of human antibodies in serum or plasma directed against Coxiella burnetii in Phase 1 or Phase 2. (qedbio.com)
  • Determining antibodies against phase I and phase II C. burnetii can help distinguish acute and chronic Q fever. (vircell.com)
  • The presence of C. burnetii DNA in swabs and the tick H. lusitanicum was determined by PCR and serum antibodies by ELISA. (jsce-ip.com)
  • Banked serum samples were secondarily tested for C. burnetii specific antibodies. (biomedcentral.com)
  • paratuberculosis (MAP) in Missouri Boer goat herds were secondarily tested for C. burnetii specific antibodies [ 14 ]. (biomedcentral.com)
  • If acute Q fever is suspected, physicians should order IgG IFA (indirect immunofluorescence assay) testing for both phase I and phase II antibodies in paired serum specimens, taken 3-6 weeks apart. (medscape.com)
  • Prevalence of peste des petits ruminants virus antibodies in sheep and goats sera from. (aosis.co.za)
Bacterium7
Infection17
  • In the veterinary field, commercial immunologic methods are the easiest to interpret and are used at the herd level to detect C. burnetii infection or exposure within a population of animals ( 3 ). (cdc.gov)
  • An enzyme-linked immunosorbent assay (ELISA) will be used to initially screen all sera specimens for IgG Phase II antibody seropositivity, a marker of acute infection. (cdc.gov)
  • Infection with Coxiella burnetii in cows usually runs without typical symptoms. (academicjournals.org)
  • La fièvre Q est-elle une infection émergente en Turquie? (who.int)
  • Hence, all isolates were genetically identical, and the infection in animals could originate from one Coxiella burnetii strain (Nine Mile RSA493 strain). (ijmm.ir)
  • Following a primary-infection, C. burnetii may persist in some patients, leading to endocarditis and vascular infections. (embrn.eu)
  • Conclusion Evidence of C. burnetii infection in beef cattle has public health implications for occupational exposure of primary producers and veterinarians and for the proximity of beef cattle properties to residential areas in regional Queensland. (qld.gov.au)
  • Q fever is a rare zoonotic infection caused by Coxiella burnetii . (hindawi.com)
  • Q fever is a rare zoonotic rickettsiosis caused by infection with C. burnetii . (hindawi.com)
  • PCR, immunohistochemistry, or culture of affected tissue can provide definitive confirmation of infection by Coxiella burnetii. (medscape.com)
  • Detection of C. burnetii DNA by polymerase chain reaction (PCR) can rapidly confirm an acute Q fever infection. (cdc.gov)
  • This area has more details on infection with Coxiella burnetii / Q Fever which is an important zoonotic disease with a wide geographical distribution. (interchem.ie)
  • Schematic outcomes of an intrauterine infection with Coxiella burnetii in a pregnant animal. (interchem.ie)
  • This study indicates that seroprevalence of C. burnetii in Boer goats raised in Missouri was low, but it does not preclude the existence of a higher level of infection in Missouri's meat goat herds. (biomedcentral.com)
  • More research is warranted to corroborate the preliminary findings reported here in order to determine the public health significance C. burnetii infection risks associated with contemporary goat production systems in the US. (biomedcentral.com)
  • A review of the burden of Q fever in the US revealed a 41.6% average prevalence of C. burnetii infection in goats [ 9 ]. (biomedcentral.com)
  • C. burnetii is also maintained in nature through an animal-tick cycle, but arthropods are not involved in human infection. (msdmanuals.com)
Serological2
  • All SERION ELISA classic Coxiella burnetii are used for the serological therapy follow-up in acute and chronic diseases. (qedbio.com)
  • Serological analysis was performed by ELISA and an immunofluorescence assay (IFA) for acute and convalescence sera of patients. (lymeresourcecentre.com)
ELISA8
  • Any sera samples positive by ELISA will then be tested by immunofluorescence antibody assay (IFA) in order to obtain end point titers for IgG to both phase I and phase II antigens. (cdc.gov)
  • 2018) Valoración de un nuevo ensayo quimioluminiscente en comparación con ELISA en la detección de IgG contra el virus de la hepatitis E. Revista de la Sociedad Andaluza de Microbiología y Parasitología Clínica. (vircell.com)
  • Description SERION ELISA classic Coxiella burnetii Phase 1 IgG/IgA resp. (qedbio.com)
  • SERION ELISA classic Coxiella burnetii IgM is recommended for the detection of acute Q-fever, while SERION ELISA classic Coxiella burnetii (Phase 2) IgG supports the differential diagnosis of infections of the respiratory tract, especially atypical pneumonia. (qedbio.com)
  • SERION ELISA classic Coxiella burnetii (Phase I) tests are recommended for the diagnosis of chronic Q-fever. (qedbio.com)
  • A total of 385 serum samples were taken from the sheep and goats from different flocks with a history of abortion and subjected to i-ELISA. (bvsalud.org)
  • The result of indirect commercial ELISA showed 13.0, 4.2 and 11.2% prevalence for Coxiella burnetii, Chlamydia abortus, and Brucella spp. (bvsalud.org)
  • Methods Serum samples were tested by ELISA for both phase II and phase I antigens of the organism using an Australian isolate. (qld.gov.au)
Specimens5
  • Serum, plasma, and urine specimens are obtained from NHANES study participants aged six years and older who have given consent for their specimens to be used in future studies. (cdc.gov)
  • and serum, urine, and a limited number of plasma specimens are available from continuous NHANES (1999-present). (cdc.gov)
  • In total, there are over 1 million combined serum, plasma, and urine specimens available. (cdc.gov)
  • Is there a time limit on how long I can use the serum, plasma, and urine specimens? (cdc.gov)
  • Dengue and dengue hemorrhagic fever were initially suspected as the cause of the outbreak but were ruled out in Nicaragua and at CDC by serologic tests and polymerase chain reaction assays of serum specimens. (cdc.gov)
Seroprevalence7
  • Since Q fever is not notifiable in many states and many human infections are inapparent, there is not reliable way of assessing how many cases of Q fever are actually occurring in the U.S Because of this, stored sera form NHANES 2003-2004 have been tested to establish baseline Q fever seroprevalence for the U.S. (cdc.gov)
  • Seroprevalence rates of less than 5% were observed for Coxiella burnetii, Echinococcus granulosus, and Toxocara canis. (nih.gov)
  • The aim of this study was to undertake a cross-sectional seroprevalence survey of Coxiella burnetii, the causative agent of Q fever, in beef cattle in Queensland. (qld.gov.au)
  • This study is the first known investigation of C. burnetii seroprevalence in beef cattle in Queensland and the first known use of an Australian C. burnetii isolate for screening using both phase II and phase I antigens. (qld.gov.au)
  • The animal and herd-level seroprevalence estimates for C. burnetii were 1.2% (3/249) and 4.2% (1/24) respectively. (biomedcentral.com)
  • This result is inconclusive because this study was disadvantaged by the small number of individual animal and herds tested, which compromised the statistical power of this study to detect a possible higher seroprevalence of C. burnetii in this population, if present. (biomedcentral.com)
  • The aim of this pilot investigation was to determine whether Boer goats, the preponderant meat goat breed in Missouri, were exposed to C. burnetii and to estimate seroprevalence of the exposure. (biomedcentral.com)
Genotyping of Coxiella2
  • Number of pig farms in the provincial administrative districts and number of farms on which pigs were sampled for the detection and genotyping of Coxiella burnetii , Gyeongsang Province, South Korea, 2014-2015. (cdc.gov)
  • Genotyping of Coxiella burnetii in sheep and goat abortion samples. (ijmm.ir)
Detection2
  • Demonstration of C. burnetii in a clinical specimen by detection of antigen or nucleic acid. (cdc.gov)
  • First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a uncared for meals security hazard in ready-to-eat raw-milk product Background: World publications on Q fever have elevated after the 2007 epidemic within the Netherlands. (atto-gentaur.com)
Zoonosis caused1
Rickettsia5
Obligate intracellular1
  • Coxiella burnetii , an obligate intracellular pathogen of both humans and animals is the causative agent of Q-fever [ 1 ]. (biomedcentral.com)
Exposure3
  • We investigated C. burnetii exposure in a remote indigenous tribe residing in the Sierra Nevada de Santa Marta, Colombia. (ajtmh.org)
  • However, there has been very little research on the prevalence of C. burnetii exposure and risk in meat goats farmed in the US. (biomedcentral.com)
  • however, an elevated serum phosphate level might indicate that an exposure has occurred. (cdc.gov)
Specimen1
  • The civilian hospital collected a serum specimen on 5 March 2018 for a C. psittaci microimmunofluorescence (MIF) immunoglobulin M (IgM) antibody test. (health.mil)
Acute Q fever2
  • In sera from acute Q fever patients, the magnitude of antiphase II titers exceeds those of antiphase I titers. (vircell.com)
  • Polymerase chain reaction (PCR) of whole blood or serum provides rapid results and can be used to diagnose acute Q fever in the first 2 weeks after symptom onset but before antibiotic administration. (medscape.com)
Molecular2
  • Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing. (ijmm.ir)
  • Unveiling the molecular mechanisms underpinning biorecognition of early-glycated human serum albumin and receptor for superior glycation finish merchandise. (atto-gentaur.com)
Agent of Q fever1
Ruminants2
  • In South Korea, there have been several studies on C. burnetii in ruminants ( 4 , 5 ), but studies evaluating C. burnetii in pigs are lacking. (cdc.gov)
  • While infected ruminants remain largely asymptomatic, the primary signs of C. burnetii include spontaneous, late-term abortions in pregnant animals. (biomedcentral.com)
Antibody1
  • Setiyono A , Subangkit M, Marea W, Santi VD, Elvira E, Fadhilah M, Aufa S. 2013. Anti Coxiella burnetii Antibody Specific for Q Fever Diagnosis Immunohistochemically in Ruminant. Proceeding of International Seminar on the Role of Veterinary Science to Support Millennium Development Goals and the 12th Asian Association of Veterinary Schools Congress. (ipb.ac.id)
Aerosols1
  • C. burnetii persists in stool, urine, milk, and tissues (especially the placenta), so that fomites and infective aerosols form easily. (msdmanuals.com)
Burnetti1
  • Positive Coxiella burnetti PCR results should be reported in Ireland to the Department of Agriculture. (interchem.ie)
Serologic3
  • the serum was separated for serologic testing. (cdc.gov)
  • The two antigenic forms of C. burnetii that are important for serologic diagnosis of Q fever are the phase I (i.e. virulent microorganism with smooth LPS [S-LPS]) and phase II (i.e. avirulent microorganism with rough LPS [R-LPS]) whole-cell antigens. (vircell.com)
  • For definitive diagnosis in the early stages of illness it is recommended to use serologic tests in combination with PCR of whole blood or serum. (cdc.gov)
Pathogen1
  • Coxiella burnetii is an underreported zoonotic pathogen in many rural regions globally. (ajtmh.org)
Infectious Disease1
Phase1
  • However, in chronic Q fever patients, the antiphase I titers exceed those of anti-phase II titers, and patients with chronic Q fever endocarditis can have high levels of serum IgA. (vircell.com)
Milk samples3
  • Khademi P, Ownagh A, Mardani K, Khalili M. PCR-RFLP of Coxiella burnetii Plasmids Isolated from Raw Milk Samples in Iran. (ijmm.ir)
  • A recent report found that 94% of bulk tank milk samples collected from US dairy herds contained C. burnetii specific DNA [ 6 ]. (biomedcentral.com)
  • Loftis and others detected C. burnetii in 42.9% (9/21) of commercial raw milk samples in the US [ 7 ], and a recent case report found Q fever clusters among raw milk consumers in the State of Michigan [ 4 ]. (biomedcentral.com)
Bacteria1
  • There is evidence that C. burnetii is a public health hazard in the US with humans being exposed to the bacteria through milk. (biomedcentral.com)
Goat2
  • Serial twofold dilutions of sera will be prepared in phosphate-buffered saline containing 1% bovine serum albumin and 1% normal goat serum. (cdc.gov)
  • C. burnetii has a worldwide occurrence with an increased prevalence in countries with dense cattle, sheep and goat populations. (idexx.com)
Goats1
  • No studies have evaluated the prevalence and risk factors of C. burnetii infections in meat goats farmed in Missouri. (biomedcentral.com)
Chronic Q-fever2
  • Anyone who was infected with C. burnetii may be at risk for developing chronic Q fever, however, people with a history of valvular defects, arterial aneurisms, or vascular grafts are at increased risk. (cdc.gov)
  • Women infected by C. burnetii during pregnancy and those with immunosuppression have also been linked to the development of chronic Q fever. (cdc.gov)
Seropositive2
  • The aim of the paper was to determine the relation between IgG concentration and the presence of C. burnetii in serum of seropositive cows. (academicjournals.org)
  • Values of different serum parameters (ALT, AST, and total protein) of seropositive animals were also determined. (bvsalud.org)
Urine2
  • Therefore, all proposals seeking the use of NHANES serum, plasma, or urine for must not produce laboratory results that are clinically significant to the NHANES participant. (cdc.gov)
  • Blood, serum, and urine samples are tested on Mondays, Wednesdays, and Thursdays with a turnaround time of 5 days. (tamu.edu)
Distinguish1
  • Several methods have been employed to identify Coxiella burnetii isolates based on the specific Coxiella burnetii QpH1 plasmid to distinguish the acute form from the chronic form of Q fever disease in humans and animals owing to the presence of unique gene sequences in this plasmid. (ijmm.ir)
Veterinary1
  • Provided veterinary and medical staff in FBiH with laboratory supplies and expertise to conduct C. burnetii IFA. (cdc.gov)
Bovine1
  • 2. Sobotta K, Hillarius K, Jiménez PH, Kerner K, Heydel C, Menge C. Interaction of Coxiella burnetii Strains of Different Sources and Genotypes with Bovine and Human Monocyte-Derived Macrophages. (ijmm.ir)
Endocarditis1
  • 6. Savinelli EA, Mallavia LP. Comparison of Coxiella burnetii plasmids to homologous chromosomal sequences present in a plasmidless endocarditis-causing isolate. (ijmm.ir)
Rickettsial1
Vaccine1
  • CSL was founded in 1916 as Commonwealth Serum Laboratories, an Australian government body focused on vaccine manufacture. (wikipedia.org)
Samples5
  • To detect C. burnetii -positive samples, we used 2 different assays and nested PCR. (cdc.gov)
  • The results of the nucleic acid sequencing of all 4 samples indicated that they had a Coxiella burnetii type (Nine Mile RSA493 strain). (ijmm.ir)
  • Further, samples were analysed for liver enzymes and total serum protein using a semi-automated chemistry analyzer. (bvsalud.org)
  • Total protein decreased for all three infections, but a significant drop was noted in Brucella positive sheep serum samples. (bvsalud.org)
  • In this study, banked serum samples from a previous study performed to estimate the apparent prevalence of Mycobacterium avium subsp. (biomedcentral.com)
Plasma1
Occurrence1
  • However, epidemiologic data on the occurrence of C. burnetii in pigs are limited. (cdc.gov)
Genotypes1
Liver enzymes1
  • Laboratory tests demonstrated a normal blood count, an elevated CRP of 244 mg/L, an accelerated erythrocyte rate of 37 mmH1, an inflammatory pattern on serum protein electrophoresis, and slightly elevated liver enzymes (AST = 46 UI/L and ALT = 36 UI/L) while liver and renal functions were normal. (hindawi.com)
Presence3
  • PCR method was used to prove the presence of C. burnetii in serum. (academicjournals.org)
  • Presence of C. burnetii in serum was from 48.6 to 66.6% in different stages of lactation and the concentration of IgG was from 76.1 to 95.4 g/L in different stages of lactation. (academicjournals.org)
  • There were small variations according to sex and age of the rabbits but significant according to the habitat (4) Conclusions: The results indicate that C. burnetii circulates freely between wild rabbits and H. lusitanicum and the sylvatic cycle in meso-Mediterranean environments relies in the presence of wild rabbits and H. lusitanicum above all if sharing habitat with red deer. (jsce-ip.com)
20181
  • A total of 86 isolates of Coxiella burnetii QpH1 plasmid, which were confirmed by the Nested-PCR method in 2018, were used to determine the RFLP panel of the QpH1 plasmid. (ijmm.ir)
NHANES1
  • All participants aged 20+ years from of NHANES 2003-2004 who gave consent for storage and future testing of their sera. (cdc.gov)
Epidemiologic1
  • Prevalence of Anaplasma phagocytophilum and Coxiella burnetii in Ixodes ricinus ticks in Switzerland: an underestimated epidemiologic risk. (unog.ch)