Serine proteases. Medical search. Frequent questions

A hydrogen bond unique to Ser190 protease-arylamidine complexes between O gamma(Ser190) and the inhibitor amidine confers an intrinsic preference for such inhibitors toward Ser190 proteases over Ala190 counterparts. (rcsb.org)
Here, we describe zebrafish that are mutant in the serine protease inhibitor Hai1a (Spint1la),which display disrupted epidermal integrity. (biologists.com)
AAF-CMK is a serine protease inhibitor which irreversibly inhibits TPPII (tripeptidyl peptidase II), a giant protease which may substitute for some proteasome functions1, and reversibly inhibits Cln2 (TPPI). (adooq.com)
Progressive neuronal and motor dysfunction in mice overexpressing the serine protease inhibitor protease nexin-1 in postmitotic neurons. (ox.ac.uk)
Protease nexin-1 (PN-1), a secreted serine protease inhibitor, is constitutively expressed in distinct neuronal cell populations of the adult CNS. (ox.ac.uk)
Serine protease inhibitor assays show that 3,4 DIC and TPCK inhibited cell growth from 79.6 to 98.4% respectively. (pacific.edu)
For the drugs used to treat viral infections, see protease inhibitor (pharmacology) . (wikipedia.org)
In medicine , protease inhibitor is often used interchangeably with alpha 1-antitrypsin (A1AT, which is abbreviated PI for this reason). (wikipedia.org)
[3] A1AT is indeed the protease inhibitor most often involved in disease, namely in alpha-1 antitrypsin deficiency . (wikipedia.org)
In-depth kinetic studies for one of the proteases (tissue factor-factor VIIa) revealed that ADP is an uncompetitive inhibitor, with K i = 0.7-2.5 mM. (isth.org)
The serine protease inhibitor is one type of protease inhibitors. (wu.ac.th)
The serine protease inhibitor gene isolated from Hevea brasiliensis leaves, a RRIT251 cultivar and designated RRIT251 H. brasiliensis protease inhibitor ( 251Hbpi) encoded a 70 amino acid protein (Figure 1). (wu.ac.th)
251HbPI is a member of the potato inhibitor I (PI-I) family of serine protease inhibitors. (wu.ac.th)
The amino acid sequence of 251HbPI was aligned with Hb-PI ( H. brasiliensis RRIM600 latex protease inhibitor), PI1 ( H. brasiliensis protease inhibitor protein 1), CI-1A (Subtilisin-chymotrypsin inhibitor-1A of H. vulgare ), CI-1B (subtilisin-chymotrypsin inhibitor-1B of Z. mays ), CI-2A (Subtilisin-chymotrypsin inhibitor-2A of H. vulgare ), and WSCI (Subtilisin-chymotrypsin inhibitor WSCI of T. aestivum ). (wu.ac.th)
2016. Cloning and characterization of a putative gene encoding serine protease inhibitor (251Hbpi) with antifungal activity against Trichophyton rubrum from Hevea brasiliensis leaves. (wu.ac.th)
Comparison of proteomic changes in the hippocampus and spinal cord of these animals revealed several members of the serine protease inhibitor (serpin) protein family are more highly expressed in P497S animals compared to either WT356 or Non-Tg controls. (umaryland.edu)
Predicted to enable serine-type endopeptidase inhibitor activity. (nih.gov)
Background: In hereditary angioedema (HAE), low levels (type 1) or defect in function (type 2) of the serine-protease inhibitor C1 Inhibitor protein results in activation of the classical pathway of the complement system as well as the contact system. (lu.se)

The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)
The aim of this thesis was to look further into the processing of such a protein by in-vestigating the activity of neutrophil serine proteases and to evaluate the processing of a protein X operated by the serine protease, Proteinase 3. (chalmers.se)
Host proteases have been shown to play important roles in many viral activities such as entry, uncoating, viral protein production and disease induction. (biomedcentral.com)
Our group had previously shown in the context of influenza infection that the human host has cell-surface proteases (called type II transmembrane serine proteases or TTSPs) that the virus requires in order to cleave a viral surface protein called hemagglutinin, itself essential for the virus to gain entry into the cell and further replicate using the host cell machinery. (cancovid.ca)
This protein is called the spike glycoprotein (or S protein) and it requires cleavage by human host cell proteases of the TTSP family for its virulence. (cancovid.ca)
Our proposal will test protease inhibitors in models where cells are expressing the S protein and the most potent molecules will then be validated in lung organoids to verify their efficacy at reducing viral propagation. (cancovid.ca)
Plasma levels of MBL-associated proteases ( MASP -1, MASP -2, MASP -3) and their regulatory protein MAp44 were compared among eGDR classifications . (bvsalud.org)
An example of enzymes that bind a single substrate and release multiple products are proteases , which cleave one protein substrate into two polypeptide products. (wikipedia.org)
Serpins function in the regulation of proteolytic cascades by entrapping and destroying proteases through a conformational switch of the protein. (umaryland.edu)
In this study, we expressed and characterized a new serine protease inhibitory protein (PI-QT) from the metagenome of sponge-associated microorganisms in Escherichia coli . (bmrat.org)
In the present study, we established a piggyBac-based transgenic system in fall webworm and generated a dominant male-sterile strain by targeting the seminal fluid protein serine protease 2 (Hcser2), displaying an outstanding trait of gSIT. (geneconvenevi.org)
Protease inhibitors and antioxidants suppressed protein kinase C and NF-kappaB activation and induction of IL-8 promoter activity in cells exposed to dust extract. (cdc.gov)
They are activated when complexed with the MANNOSE-BINDING LECTIN, therefore also known as Mannose-binding protein-Associated Serine Proteases (MASPs). (bvsalud.org)

Serine proteases (or serine endopeptidases) are enzymes that cleave peptide bonds in proteins. (wikipedia.org)
In biochemistry , serine proteases or serine endopeptidases (newer name) are a class of peptidases ( enzymes that cleave peptide bonds in proteins ) that are characterised by the presence of a serine residue in the active site of the enzyme . (bionity.com)
The blotched snakehead virus (BSNV), an aquatic birnavirus, encodes a polyprotein (NH2-pVP2-X-VP4-VP3-COOH) that is processed through the proteolytic activity of its own protease (VP4) to liberate itself and the viral proteins pVP2, X and VP3. (rcsb.org)
It has been suggested that these proteases are re-sponsible for the cleavage of di˙erent proteins, that act as regulators of the immune system. (chalmers.se)
In biology and biochemistry , protease inhibitors , or antiproteases , [1] are molecules that inhibit the function of proteases ( enzymes that aid the breakdown of proteins ). (wikipedia.org)
Many naturally occurring protease inhibitors are proteins . (wikipedia.org)
Serine protease inhibitors have been related to the mobilization of storage proteins, regulation of endogenous enzymatic activities, and stabilization of defense proteins against microorganisms, insects, and animals. (wu.ac.th)
These proteins are suggested as T. rubrum serine protease. (wu.ac.th)

The present work focused on the characterization of two relevant HA cleaving type-II transmembrane serine proteases matriptase and TMPRSS2. (uni-marburg.de)
With a TMPRSS2 dependancy of HA-activation of certain subtypes, the characterization of this protease is an important prerequisite for being available as a target for influenza drug design. (uni-marburg.de)
In this work, a general biochemical characterization in snake venom of serine proteases (SVSP), phospholipases A 2 (PLA 2 ), metalloproteases (SVMP) and hyaluronidases (SVH) of Crotalus aquilus (Ca), Crotalus polystictus (Cp) and Crotalus molossus nigrescens (Cmn) was done. (mdpi.com)
Other viper venom serine proteases have been shown to exert distinct biological effects, and our preliminary functional characterization of rhinocerase suggest it to be multifunctional. (reading.ac.uk)

The triad is located in the active site of the enzyme, where catalysis occurs, and is preserved in all superfamilies of serine protease enzymes. (wikipedia.org)
The whole reaction can be summarized as follows: The polypeptide substrate binds to the surface of the serine protease enzyme such that the scissile bond is inserted into the active site of the enzyme, with the carbonyl carbon of this bond positioned near the nucleophilic serine. (wikipedia.org)
Located very near one another near the heart of the enzyme, these three key amino acids each play an essential role in the cleaving ability of the proteases. (bionity.com)
Chymotrypsin-targeting FLISP probes interact with active catalytic sites of chymotrypsin-like proteases, so cells with greater quantities of active chymotrypsin-like enzyme activity with a greater fluorescence potential than those that are not upregulated. (immunochemistry.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Protease, Serine 23 (PRSS23) in serum, plasma, tissue homogenates and other biological fluids. (worldcarecouncil.org)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Protease, Serine 23 (PRSS23) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (worldcarecouncil.org)
There are several illustrations of serine peptidase enzyme and one of them is alpha-Chymotrypsin which is one of the known enzymes in the Trypsin household. (graduateway.com)
These enzymes are believed to be 'serine peptidase ' enzymes which means they all originate from the same beginning and due to evolution these enzyme has been meeting off from each others way and both enzyme adapted in its environment in order to last. (graduateway.com)
At pH 10 to 11, enzyme activity was high, and alkaline protease was confirmed after incubation for up to 2 and 20 hours at the same pH range. (digiwire.co.in)
Metal ions such as Ca+2, Mg+2, and Mn+2 significantly increased enzyme activity, but phenylmethylsulphonyl fluoride (PMSF) reduced enzyme activity completely, and diisopropyl fluorophosphates (DFP) decreased enzyme activity up to 92 percent, confirming serine protease. (digiwire.co.in)
These agents interfere with HCV replication by inhibiting a key viral enzyme, NS3/4A serine protease. (medscape.com)

Mannan-binding lectin associated serine protease (MASP-2) is homologous to MASP-1 and the two C1q-associated serine proteases C1r and C1s. (lu.se)
Plasma levels of mannan-binding lectin-associated serine proteases are increased in type 1 diabetes patients with insulin resistance. (bvsalud.org)

UTP, GTP and CTP showed protease inhibition comparable to ATP. (isth.org)
IC 50 for inhibition of serine proteases by ATP ranged from 2-11 mM. (isth.org)
Inhibition of serine proteases by nucleotides could serve as a cellular safety mechanism to keep any prematurely activated proteases in the lumen of the ER and Golgi quiescent prior to secretion. (isth.org)
These effects were blocked by heat inactivation or by serine protease inhibition of A. alternata filtrates, and mimicked by PAR 2 specific ligands SLIGRL-NH 2 or 2-furoyl-LIGRLO-NH 2 , but not the PAR 1 -specific ligand TFLLR-NH 2 . (arizona.edu)
Protease inhibition analysis revealed that 251HbPI inhibited the activity of chymotrypsin and subtilisin A but did not trypsin protease. (wu.ac.th)

Serine proteases fall into two broad categories based on their structure: chymotrypsin-like (trypsin-like) or subtilisin-like. (wikipedia.org)
Trypsin-like proteases cleave peptide bonds following a positively charged amino acid (lysine or arginine). (wikipedia.org)
The S1 pocket of chymotrypsin-like enzymes is more hydrophobic than in trypsin-like proteases. (wikipedia.org)
Elastase-like proteases have a much smaller S1 cleft than either trypsin- or chymotrypsin-like proteases. (wikipedia.org)
The three serine proteases of the chymotrypsin-like clan that have been studied in greatest detail are chymotrypsin , trypsin , and elastase . (bionity.com)
Trypsin-like serine proteases are crucial for influenza activation by mediating the cleavage of the viral surface glycoprotein HA and hence promoting the fusion potential of the virus. (uni-marburg.de)
Involved or implicated in a wide spectrum of diseases, trypsin-like serine proteases comprise well studied drug targets and anti-targets that can be subdivided into two major classes. (rcsb.org)
Alice: Trypsin (serine protease) inhibitors in peanut genotypes aiming for control of stored grain pests. (embrapa.br)
The chief feature of a serine peptidase is that they have a alone Ser residue of exceeding responsiveness that forms a covalent bond with some of the substrates or inhibitors ( Creighton J ) . In both Trypsin and subtilisin shows many similarity and both contains the peculiarly extremely reaction serine residue which can be specifically phosphorylated by other substrate such as isoprophylfluorophosphate ( Alden and Wright, 1970 ) , sulphonated and acylated utilizing different enzymes. (graduateway.com)
Results: Dust extracts contained trypsin and elastase activities, and activated protease activated receptor (PAR)-1 and -2. (cdc.gov)

Polyclonal Antibody to Transmembrane Protease, Serine 2 (TMPRSS2) is Available at Gentaur Genprice with the fastest delivery. (proteomecommons.org)

Despite low levels of sequence identity, VP4 shows similarities in its active site to other characterized Ser/Lys proteases such as signal peptidase, LexA protease and Lon protease. (rcsb.org)
In this essay I will be reexamining the construction and map of a serine peptidase known as subtilisin and I will analyse the different techniques used by different research workers to specify the construction or maps of Subtilisin. (graduateway.com)
Subtilisins are illustration of serine peptidase these are found in every being that exists. (graduateway.com)

Protease inhibitors may be classified either by the type of protease they inhibit, or by their mechanism of action. (wikipedia.org)

Description: This Human Serine protease 23 (PRSS23) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS23. (worldcarecouncil.org)
Description: A sandwich ELISA kit for detection of Protease, Serine 23 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (worldcarecouncil.org)

Serine proteases participate in a wide range of functions in the body, including blood clotting , immunity , and inflammation , as well as contributing to digestive enzymes in both prokaryotes and eukaryotes. (bionity.com)
Serine peptidases are extremely specific enzymes as they use His, Ser and Asp portion of their active site. (graduateway.com)
Proteases are enzymes that catalyze the hydrolysis of peptide bonds and play an important role in almost all biological processes. (bmrat.org)

This is the first crystal structure of a birnavirus protease and the first crystal structure of a viral protease that utilizes a lysine general base in its catalytic mechanism. (rcsb.org)

From research revealed that 251HbPI inhibits Trichophyton rubrum (dermatophyte infection in humans) growth, suggesting that 251HbPI inhibits T. rubrum serine protease, which is an important virulence factor secreted during host infection of T. rubrum . (wu.ac.th)
Simeprevir inhibits HCV NS3/4A protease needed for proteolytic cleavage of the HCV-encoded polyprotein into mature forms. (medscape.com)

Within each superfamily, families are designated by their catalytic nucleophile, (S: serine proteases). (wikipedia.org)
Serine proteases are characterised by a distinctive structure, consisting of two beta-barrel domains that converge at the catalytic active site. (wikipedia.org)
Subtilisin is evolutionarily unrelated to the chymotrypsin-clan, but shares the same catalytic mechanism utilising a catalytic triad, to create a nucleophilic serine. (wikipedia.org)
The main player in the catalytic mechanism in the serine proteases is the catalytic triad. (wikipedia.org)
We report here the crystal structure of a VP4 protease from BSNV, which displays a catalytic serine/lysine dyad in its active site. (rcsb.org)
Serine proteases catalyze a multi-step covalent catalytic mechanism of peptide bond cleavage. (escholarship.org)

Nucleotides Inhibit Serine Proteases Involved in Blood Clotting and Other Cellular Processes [abstract]. (isth.org)

In this study, subtilisin-like serine proteases (subtilases) were examined for their putative role in cell viability. (pacific.edu)
Therefore, these cellular proteases are putative targets for the development of antivirals that inhibit their activity. (biomedcentral.com)

Serine proteases are grouped into clans that share structural homology and then further subgrouped into families that share close sequence homology. (bionity.com)
High-temperature-requirement A2 (HtrA2)/Omi/PARK13 is a serine protease with extensive homology to the Escherichia coli HtrAs that are required for bacterial survival at high temperatures. (benthamscience.com)

These immunoregulative functions are in part conveyed by the release of cytokines and chemokines as well as neutrophil derived proteases. (chalmers.se)
This thesis is a part of a larger project, and is based on previous results regarding the act of neutrophil serine proteases. (chalmers.se)
Neutrophil cytoplasts, which are neutrophils devoid of intracellular granular compartments, were prepared and used in the experiments as models for neutrophils with limited serine protease activity. (chalmers.se)

Subtilisin is a serine protease in prokaryotes. (wikipedia.org)

The ancestral gene that gave rise to the AFGP gene was that of a trypsinogen-like protease (TLP), but whether TLP is the trypsinogen that performs digestive function in the notothenioids is not known. (sicb.org)
Transmembrane serine protease 3 (TMPRSS3) is the most common causative hearing loss gene in adults undergoing cochlear implantation and a significant cause of non-syndromic hearing loss. (iupui.edu)
Mutation of the seminal protease gene, serine protease 2, results in male sterility in diverse lepidopterans. (ox.ac.uk)
Proteases and oxidant stress control organic dust induction of inflammatory gene expression in lung epithelial cells. (cdc.gov)
We studied the mechanisms by which protease activities in poultry dust extracts and intracellular oxidant stress induce inflammatory gene expression in A549 and Beas2B lung epithelial cells. (cdc.gov)
Serine protease inhibitors and PAR-1 or PAR-2 knockdown suppressed inflammatory gene induction. (cdc.gov)
Conclusions: Our studies demonstrate that proteases and intracellular oxidants control organic dust induction of inflammatory gene expression in lung epithelial cells. (cdc.gov)

The majority belong to the S1 family of the PA clan (superfamily) of proteases. (wikipedia.org)
For superfamilies, P: superfamily, containing a mixture of nucleophile class families, S: purely serine proteases. (wikipedia.org)

The defects of hai1 mutants can be phenocopied by overexpression and can be fully rescued by simultaneous inactivation of the serine protease Matriptase1a(St14a), indicating that Hai1 promotes epithelial integrity by inhibiting Matriptase1a. (biologists.com)

Together, the structure of VP4 provides insights into the mechanism of a recently characterized clan of serine proteases that utilize a lysine general base and reveals the structure of potential targets for antiviral therapy, especially for other related and economically important viruses, such as infectious bursal disease virus in poultry and infectious pancreatic necrosis virus in aquaculture. (rcsb.org)
Protease inhibitors in organisms are produced for regulating the proteolytic activity of their targets. (wu.ac.th)

Each amino acid in the triad performs a specific task in this process: The serine has an -OH group that is able to act as a nucleophile, attacking the carbonyl carbon of the scissile peptide bond of the substrate. (wikipedia.org)
The serine has an -OH group that is able to act as a nucleophile, attacking the carbonyl carbon of the scissile peptide bond of the substrate. (bionity.com)

The partial amino acid sequence shows similarity to other viper venom serine proteases, but is clearly distinct from the sequence of the only other sequenced serine protease from Bitis gabonica. (reading.ac.uk)

Quantitate intracellular chymotrypsin-like serine protease activity in vitro with ICT's FLISP kits. (immunochemistry.com)

Several protease inhibitors, representing different classes of proteases (AEBSF, Pepstatin A, E-64, TPCK, PMSF and aprotinin), were tested for inhibitory effects on an RSV A2 infection of HEp-2 cells. (biomedcentral.com)

Many highly-toxic thrombin-like serine protease isoforms are found in snake venoms. (wikipedia.org)
It has long been assumed that serine proteases including thrombin carry-out catalysis without significant conformational rearrangement of their stable two-β-barrel structure. (escholarship.org)

The isolation, purification, and partial characterisation of thermostable serine alkaline protease generated by Bacillus thuringiensis were described in this study. (digiwire.co.in)

One strategy of influenza research is to focus on host-specific proteases that are essential for virus activation and spread. (uni-marburg.de)
Host proteases have been described to play essential roles in Ebola, HCV, HIV and influenza, such that specific protease inhibitors are able to reduce infection. (biomedcentral.com)

Serum serine proteases which participate in COMPLEMENT ACTIVATION. (bvsalud.org)

We examined the impact of proteases from A. alternata on lung inflammation in vivo and on cleaving protease-activated receptor-2 (PAR 2 ) in vitro. (arizona.edu)
A. alternata filtrate applied to the airway in nonsensitized Balb/c mice induced a protease-dependent lung inflammation. (arizona.edu)
Transmembrane serine protease 2 is a prognostic factor for lung adenocarcinoma. (cdc.gov)

Because of their supporting role in the apoptotic process, serine protease activity will be greater in apoptotic cell populations compared to healthy cells of the same type. (immunochemistry.com)
FLISP may be used in combination with FLICA to discriminate serine protease activity from caspase activity in the same cell. (immunochemistry.com)
Serine protease activity was eval-uated with the help of activity assays. (chalmers.se)
The experimental model was validated under 14 degrees C, pH 7.54, and 0.55% yeast extract, showing a protease activity of 447.65 +/- 2.6 U/mL by a prediction model of 445.48 U/mL. (unesp.br)
We hypothesize that low mM concentrations of ATP and other nucleotides in the ER and Golgi lumen could stabilize nascent serine proteases (especially, any prematurely activated zymogens) by inhibiting their enzymatic activity prior to secretion. (isth.org)
Investigate how physiologic concentrations of nucleotides effect serine protease activity. (isth.org)
Low mM concentrations of ATP or ADP strongly inhibited the activity of most serine proteases in our screen. (isth.org)
Allergens associated with asthma (asthmagens) share a common protease activity that may directly impact respiratory epithelial biology and lead to symptoms of asthma. (arizona.edu)
In these cases, protease inhibitors can be used as one of versatile tools for regulating proteolytic activity of target proteases as well as therapeutic applications. (bmrat.org)
The best temperature for protease activity was 45°C, with thermal stability of 100 percent evaluated after 350 minutes of incubation. (digiwire.co.in)

We have now cloned and sequenced full length cDNA of both pancreatic TLPs and digestive trypsinogens across notothenioid families, thereby establishing that they are two distinct groups of pancreatic proteases. (sicb.org)
Phylogenetic analyses also reveal separate grouping of digestive trypsinogens, TLPs and other serine proteases. (sicb.org)

RSV infection can be inhibited by broad serine protease inhibitors, AEBSF and TPCK. (biomedcentral.com)

RSV utilizes a host protease in its replication cycle but its potential as antiviral target is unknown. (biomedcentral.com)

These data demonstrate that A. alternata proteases act through PAR2 to induce rapid increases in human airway epithelial [Ca 2+ ] i in vitro and cell recruitment in vivo. (arizona.edu)

This study will form the basis for future research to understand the mechanisms of serine protease action, and examine the potential for rhinocerase to be used clinically to reduce the risk of human haemostatic disorders such as heart attacks and strokes. (reading.ac.uk)

The MEROPS protease classification system counts 16 superfamilies (as of 2013) each containing many families. (wikipedia.org)
In 2004 Rawlings and colleagues introduced a classification of protease inhibitors based on similarities detectable at the level of amino acid sequence. (wikipedia.org)

Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire. (crcm-marseille.fr)

A pair of electrons on the histidine nitrogen has the ability to accept the hydrogen from the serine -OH group, thus coordinating the attack of the peptide bond. (wikipedia.org)
The serine -OH attacks the carbonyl carbon, and the nitrogen of the histidine accepts the hydrogen from the -OH of the [serine] and a pair of electrons from the double bond of the carbonyl oxygen moves to the oxygen. (wikipedia.org)
histidine (His 57), serine (Ser 195) (hence the name "serine protease") and aspartic acid (Asp 102). (bionity.com)

Serine serves as the nucleophilic amino acid at the (enzyme's) active site. (wikipedia.org)
The triad is a coordinated structure consisting of three amino acids: His 57, Ser 195 (hence the name "serine protease") and Asp 102. (wikipedia.org)
These three key amino acids each play an essential role in the cleaving ability of the proteases. (wikipedia.org)

A 2(3) factorial design was applied to optimize the protease production using three independent variables, namely temperature, pH and concentration of yeast extract, was also used a Central Composite Design (CCD) under Response Surface Methodology (RSM). (unesp.br)

Anatomy12

Organisms17

Chemicals and Drugs153

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes47

Information Science4

Health Care1

Inhibitor18

Protein13

Proteins8

Characterization4

Enzyme11

Mannan-binding lectin-as2

Inhibition5

Trypsin10

Transmembrane Protease1

Peptidase3

Type of protease1

ELISA Kit2

Enzymes3

Viral1

Inhibits2

Catalytic6

Abstract1

Putative2

Homology2

Neutrophil3

Subtilisin1

Gene7

Superfamily2

Inactivation1

Targets2

Substrate2

Venom1

Intracellular1

PMSF1

Thrombin2

Characterisation1

Influenza2

Serum1

Lung3

Activity10

Digestive2

TPCK1

Utilizes1

Vitro1

Mechanisms1

Classification2

Receptor1

Histidine3

Amino3

Temperature1