SerineThreoninePhosphorylationAmino Acid SequenceMolecular Sequence DataPhosphoserinePhosphothreonineMutagenesis, Site-DirectedProtein-Serine-Threonine KinasesBinding SitesProtein KinasesSerine EndopeptidasesCell LineMutationProtein BindingKineticsSequence Homology, Amino AcidProtein Structure, TertiaryTyrosineBase SequencePeptide MappingSubstrate SpecificityEnzyme ActivationPhosphoproteinsRecombinant ProteinsPhosphopeptidesModels, MolecularAmino Acid SubstitutionProtein Processing, Post-TranslationalProtein ConformationProtein Kinase CPeptide FragmentsAmino AcidsCloning, MolecularTransfectionStructure-Activity RelationshipCasein Kinase IIPhosphoprotein PhosphatasesElectrophoresis, Polyacrylamide GelRecombinant Fusion ProteinsSignal TransductionPhosphoamino AcidsSerine ProteasesSequence AlignmentSerine Proteinase InhibitorsCells, CulturedProtein-Tyrosine KinasesEscherichia coliCOS CellsCyclic AMP-Dependent Protein KinasesPeptidesAmino Acid MotifsEnzyme InhibitorsThreonine DehydrataseAlanineProtein Structure, SecondaryMolecular WeightBlotting, WesternPhosphotyrosineTrypsinHeLa CellsCalcium-Calmodulin-Dependent Protein KinasesProteinsCysteineCatalysisCatalytic DomainCattleDNA-Binding ProteinsPrecipitin TestsOkadaic AcidMembrane ProteinsConserved SequenceGlycosylationCasein KinasesCell MembranePoint MutationMass SpectrometryProtein Phosphatase 2Carrier ProteinsDNA PrimersAcetylglucosamineCricetinaeProto-Oncogene ProteinsBacterial ProteinsOxidative PhosphorylationMitogen-Activated Protein KinasesProto-Oncogene Proteins c-akt14-3-3 ProteinsChromatography, High Pressure LiquidImmunoblottingIsoenzymesDNAIntracellular Signaling Peptides and ProteinsMutagenesisPhosphorus RadioisotopesAdenosine TriphosphateDNA, ComplementaryProtein Phosphatase 1ImmunoprecipitationProtein Transport