SerineThreoninePhosphorylationAmino Acid SequenceMolecular Sequence DataProtein-Serine-Threonine KinasesProtein KinasesPhosphoserinePhosphothreonineMutagenesis, Site-DirectedBinding SitesMutationCell LineProtein BindingKineticsProtein Kinase CTyrosineSerine EndopeptidasesProtein Structure, TertiarySubstrate SpecificityBase SequenceSequence Homology, Amino AcidEnzyme ActivationPhosphoproteinsRecombinant ProteinsPeptide MappingPhosphatidylinositol 3-KinasesCalcium-Calmodulin-Dependent Protein KinasesCyclic AMP-Dependent Protein KinasesCasein Kinase IIMAP Kinase Signaling SystemModels, MolecularPhosphopeptidesProtein-Tyrosine KinasesAmino Acid SubstitutionTransfectionProtein Processing, Post-TranslationalSignal TransductionProtein ConformationCloning, MolecularRecombinant Fusion ProteinsProtein Kinase InhibitorsPhosphoprotein PhosphatasesAmino AcidsStructure-Activity RelationshipElectrophoresis, Polyacrylamide GelPeptide FragmentsCells, Culturedsrc-Family KinasesCasein KinasesSequence AlignmentMitogen-Activated Protein Kinase 1Blotting, WesternPhosphoamino AcidsEnzyme InhibitorsEscherichia coliSerine ProteasesPeptidesPhosphotyrosineMitogen-Activated Protein KinasesSerine Proteinase InhibitorsAmino Acid MotifsCDC2 Protein KinaseHeLa CellsMolecular WeightIsoenzymesProto-Oncogene ProteinsMitogen-Activated Protein Kinase 3ProteinsCOS CellsPrecipitin Testsp38 Mitogen-Activated Protein KinasesProto-Oncogene Proteins c-aktMitogen-Activated Protein Kinase KinasesDNA-Binding ProteinsProtein Structure, SecondaryAlanineCatalytic DomainTrypsinCatalysisCattleMembrane ProteinsGlycogen Synthase Kinase 3Intracellular Signaling Peptides and ProteinsThreonine DehydrataseOkadaic AcidCell MembraneCarrier ProteinsCysteineImmunoblottingTetradecanoylphorbol AcetateDNA PrimersCyclin-Dependent KinasesConserved SequenceProtein Phosphatase 2Point MutationCricetinaeAdenosine TriphosphateCalciumTumor Cells, Cultured