Sera by indirect. Medical search. Frequent questions

Parasite-infected-cell-agglutination and indirect immunofluorescence assays for detection of human serum antibodies bound to antigens on Plasmodium falciparum-infected erythrocytes. (ox.ac.uk)
Two methods are described for detecting the binding of serum antibodies from adults in an endemic malarious area (The Gambia) to surface antigens on Plasmodium falciparum-infected erythrocytes. (ox.ac.uk)
In contrast to the results with freshly collected isolates, antibodies from sera of Gambian adults did not bind to the surface of infected cells from five different culture-adapted isolates of P. falciparum. (ox.ac.uk)
Title : Rabies Antibodies In Human Serums Titrated By The Indirect Fa Method Personal Author(s) : Leffingwell, L.;Irons, J. V. (cdc.gov)
Of the 54,911 sera routinely tested for anti-double stranded-DNA antibodies (Ab), 2,297 gave a positive reaction with both indirect immunofluorescence (IIF) on Crithidia luciliae (CL) and the Farr test, or with only one of the two tests. (nih.gov)
Among the discrepant Farr test(-) IIF-CL(+) sera, 1/3 corresponded to false positive reactions IIF-CL and 2/3 of the remaining sera contained weakly avid anti-ds-DNA antibodies undetectable using the Farr test. (nih.gov)
The IDEXX BVDV Total Ab X3 Test is an enzyme immunoassay (ELISA) designed to detect BVDV antibodies in serum and plasma samples. (idexx.com)
Assays that have been applied to the detection of antibodies are classically referred to as indirect immunoassays, which include three different formats: indirect, double-antigen sandwich, and total IgG capture. (rsc.org)
We compare the performances of these indirect immunoassays by developing assays for HIV antibodies in human serum. (rsc.org)
Testing for the presence of antineural antibodies in both serum and CSF may improve diagnostic yield. (arupconsult.com)
The IDEXX ORT Ab Test is an indirect ELISA for the detection of ORT antibodies in chicken and turkey serum. (idexx.co.uk)
Indirect immunofluorescence and ELISA can measure desmoglein antibodies in blood serum. (wikidoc.org)
If acute Q fever is suspected, physicians should order IgG IFA (indirect immunofluorescence assay) testing for both phase I and phase II antibodies in paired serum specimens, taken 3-6 weeks apart. (medscape.com)

Indirect serum bilirubin measurement is used to evaluate liver function and jaundice. (athenslab.gr)
There are three types of bilirubin: total, direct (conjugated) and indirect (unconjugated). (athenslab.gr)
Total bilirubin is made up of the sum of direct bilirubin plus indirect bilirubin. (athenslab.gr)
Indirect bilirubin , also known as free or unbound bilirubin, is usually found in the bloodstream. (athenslab.gr)
Indirect bilirubin is increased in cases of hemolytic jaundice, in which the degradation of hemoglobin results in higher levels of indirect bilirubin. (athenslab.gr)
If total bilirubin measurement is not possible, further testing is carried out to determine the level of direct and indirect bilirubin. (athenslab.gr)
Practice guidelines have been created to assist with this clinical situation and have recommended that interpretation of serum total bilirubin measurements must consider the age of the infant in hours in addition to other risk factors such as gestational age. (aacc.org)
Most automated assays determine total, direct and indirect (calculated) bilirubin. (aacc.org)
Calibration of these methods is complicated by the use of bovine serum, instead of human serum, enriched with unconjugated bilirubin and ditauraobilirubin. (aacc.org)
For diazo based methods, unconjugated bilirubin in bovine serum reacts incompletely and unpredictably. (aacc.org)
Therefore it is virtually impossible to assign accurate bilirubin calibrators using bovine serum as a protein base! (aacc.org)
Nevertheless, many laboratories have done this comparison and implemented the device with a cutoff, above which a confirmatory measurement using a serum total bilirubin is requested. (aacc.org)
Measure serum ALT, AST, and bilirubin prior to initiation of eltrombopag, every 2 weeks during the dose adjustment phase, and monthly following establishment of a stable dose. (salesandmarketingnetwork.com)
Contraction index was negatively correlated with serum total bilirubin. (who.int)
Impaired gall bladder motility was evident in patients with beta- thalassaemia and it may be related to disease duration, serum ferritin and total serum bilirubin level. (who.int)

An antibody-mediated parasite-infected-cell-agglutination assay (without secondary antibody) and an indirect immunofluorescence assay employing an anti-Fc secondary reagent were used to detect bound antibody. (ox.ac.uk)
Serum from 'non-immune' Europeans did not agglutinate infected erythrocytes, however, in the immunofluorescence test with anti-Ig and anti-F(ab')2 secondary reagents we could detect the binding of IgG antibody from 'non-immune' European serum to a small proportion of infected cells. (ox.ac.uk)
The IDEXX PM Ab Tests are enzyme-linked immunosorbent assay (ELISA) designed to detect antibody to Pasteurella multocida in chicken serum. (idexx.com)
An assessment of immune status, as well as serologic identification of PM, requires a measurement of antibody in serum. (idexx.com)
Each of these three approaches relies on a unique pair of reagents to capture the target antibody and transduce a detectable signal, which permits assays to be tuned for ideal performance based on the availability and quality of reagents, the resources available to the operator, and the complexity of the sample ( e.g. , serum vs. saliva). (rsc.org)
Low glutamic acid decarboxylase 65-kd isoform (GAD65) antibody titers in serum should be followed by CSF testing to confirm the diagnosis. (arupconsult.com)
An assessment of immune status, as well as serologic identification of ORT, requires a measurement of antibody to ORT in serum. (idexx.co.uk)
In the present study, we have established human cell lines that stably express human AQP4 and used these cells to detect and titrate anti-AQP4 antibody present in the sera of patients with NMO by immunofluorescence assay. (go.jp)
The staining pattern of AQP4-expressing cells treated with each serum of these eight NMO patients corresponded to that with a commercially available anti-AQP4 antibody. (go.jp)
Antibody responses, including isotypes, were assessed by virus neutralization (VN) and indirect fluorescent antibody (IFA) tests over time before and after re-challenge. (usda.gov)
Euthyroid, but TPO or Tg antibody positive, pregnant women should have measurement of serum TSH concentration performed at time of pregnancy confirmation, and every 4 wk through mid-pregnancy. (medscape.com)

furthermore, serum levels of various biomarkers change, in addition to the appearance of new biomarkers in the serum during the different stages of fibrosis [ 7 , 8 , 15 ]. (hindawi.com)
Aiming to provide proof-of-concept evidence and methodology for the potential role of serum-derived EVs as biomarkers in leukemic versus healthy samples in this study, we hope to pave the way for future detection of promising biomarkers for imminent disease progression and the identification of potential targets to be used in a therapeutic strategy. (nature.com)
ntroduction: Indirect serum biomarkers present an acceptable noninvasive and cheap alternative for screening of significant liver fibrosis (SLF). (bvsalud.org)
The diagnostic value of indirect serum biomarkers for diagnosis of SLF was evaluated using the area under the receiver operating characteristics curve (AUROC) using Fibroscan® as gold standard. (bvsalud.org)
Conclusion: Despite APRI and FIB-4 being more likely to identify participants with SLF, the overall diagnostic value of all serum biomarkers was poor with and without stratification by HIV status. (bvsalud.org)
no validated serum biomarkers currently exist. (lu.se)
The serum biomarkers proposed to date have not yet demon- pendent test cohort (prevalidation). (lu.se)
The results from this prevali- the field of cancer biomarkers (11-13), and that approach was dation study showed that patients could be classified into high- adopted here to define predictive serum biomarkers associated versus low-risk groups for developing metastatic breast cancer with tumor relapse in breast cancer patients. (lu.se)

The objective of this studywas to evaluate diagnostic performance and discriminative ability of Rose Bengal Plate Test (RBPT), complement fixation test (CFT), and indirect enzyme linked immunosorbent assay (I-ELISA) tests used for screening and confirmatory diagnosis of bovine brucellosis in Ethiopia using Bayesian method. (hindawi.com)
Purified oocysts were used as antigens in the indirect immunofluorecence assay. (scielo.br)
The indirect immunoflorescence assay, when used with other parasitological techniques appears to be useful for retrospective population studies and for diagnosis of acute infection. (scielo.br)
Serum samples from the subjects were tested for rickettsial infections using indirect micro-immunofluorescence assay. (who.int)

Serum concentrations of human C-reactive protein (CRP) were measured via ELISA to assess inflammation. (wku.edu)

Patients may have decreased serum haptoglobin and elevated reticulocyte counts. (medscape.com)

High concentrations of haptocorrin interfere with routine measurement of cobalamins in human serum and milk. (kcl.ac.uk)
The accuracy of serum free T4 measurement by the indirect analog immunoassays is influenced by pregnancy and also varies significantly by manufacturer. (medscape.com)

Serum or plasma vitamin B12 has been measured using commercially available competitive protein binding immunoassays (CPBA). (kcl.ac.uk)
Special EV surface protein patterns in serum have the potential as noninvasive biomarker candidates to distinguish several disease-related patterns ex vivo or in vivo. (nature.com)
6] Thus, a low baseline serum TIBC is associated with iron deficiency, protein emerge malnutrition, inflammation, a poor quality of life, and increased risk of mortality. (medscape.com)
In this respect, in contrast to predictors based for each serum protein was determined by comparing the samples upon tumor characteristics at the time of surgery, serum is a par- collected at the primary operation and then 3-6 mo later. (lu.se)
Conse- demonstrated that this protein signature provided an added value quently, using minute amounts of nonfractionated serum (14) and compared with conventional clinical parameters. (lu.se)

however, most symptomatic serum samples submitted as their primary specimen persons have fever, myalgia, and headache associated because serology is the standard diagnostic method with thrombocytopenia, leukopenia, and elevated for Lyme disease, the most common tickborne infec- transaminase levels ( 3 , 6 ). (cdc.gov)
Laboratory diagnosis of HGA can be estab- The Study lished by using microscopy, serology, or nucleic We tested 2 different serum specimen groups for A. acid amplification test (NAAT) ( 3 , 11 ). (cdc.gov)

All participants underwent an indirect calorimetry to measure the respiratory quotient. (frontiersin.org)
Fat oxidation can be assessed clinically by measuring respiratory quotient (RQ), which is the ratio of the carbon dioxide expired to the oxygen consumed during indirect calorimetry. (frontiersin.org)
REE was assessed via 35 minutes of indirect calorimetry. (wku.edu)

Serum creatinine measurements should be obtained to assess potential renal insufficiency. (medscape.com)
Increased if poor kidney function so often requires serum creatinine analysis as well. (kcl.ac.uk)
We measured serum lipids and creatinine and transaminases at basal and follow-up visit. (researchgate.net)

The serum dilution titers were reproducible in blinded studies. (go.jp)

Various direct and indirect tests may be useful for diagnosis (see below), though the results of laboratory studies may be unremarkable in individuals who are asymptomatic. (medscape.com)

Reciprocal of highest serum dilution showing flourescence. (cdc.gov)
Reciporcal of highest serum dilution with neutalizing 100 TCID50 of virus in 50% of the wells. (cdc.gov)

serology as a nonspecific febrile illness can lead to lack of rec- in Canada to determine if serum samples could be ognition, and delays in antimicrobial administration an acceptable alternative to whole blood for the di- can cause illness and death ( 9 , 10 ). (cdc.gov)
We isolated DNA from 100 L of serum by us- tested positive samples to ensure reproducibility. (cdc.gov)
A total of 278 sera samples from five dairy herds were collected and tested. (hindawi.com)
Serum - Hemolysis Samples - Tier 1. (leebio.com)
Serum - Hemolysis Samples - Tier 3. (leebio.com)
Serum - Lipemic Samples - Tier 1. (leebio.com)
EVs collected from the serum of leukemic samples might correlate with disease stage, drug-/immunological resistance, or might correlate with antileukemic immunity/immune response. (nature.com)
Serum samples of all five follow-up cases (100%) had strong-positive IgG responses to scrub typhus. (who.int)

EVs were isolated from the serum of acute myeloid leukemia (AML), acute lymphoid leukemia (ALL), chronic lymphoid leukemia (CLL) patients, and healthy volunteers. (nature.com)

The concentration of serum IgE in four human helminthiases was determined by an indirect single radial immunodiffusion method. (ajtmh.org)
The results clearly indicate that an increase of serum IgE concentration occurs in various helminthic infections, although the significance of this is still obscure. (ajtmh.org)
Evaluation of serum TSH concentration is recommended for all women seeking care for infertility. (medscape.com)
1, 7] A low serum iron concentration is typically present along with a normal transferrin saturation in individuals with anemia of chronic inflammation. (medscape.com)

In the later stages of chronic pancreatitis, atrophy of the pancreatic parenchyma can result in normal serum enzyme levels because of significant fibrosis of the pancreas, resulting in decreased concentrations of these enzymes within the pancreas. (medscape.com)
Although low concentrations of serum trypsin are relatively specific for advanced chronic pancreatitis, they are not sensitive enough to be helpful in most patients with mild to moderate disease. (medscape.com)

These serum and cerebrospinal fluid (CSF) autoimmune stiff-person disorders panels can be used for the evaluation of patients with stiffness, spasms, hyperekplexia, limb rigidity, myoclonus, or limb dystonia in the presence or absence of encephalopathy. (arupconsult.com)
Indirect assessment of 4,4'-diphenylmethane diisocyanate (MDI) exposure by evaluation of specific humoral immune responses to MDI conjugated to human serum albumin. (cdc.gov)

Laboratory studies to identify the causative factors of chronic pancreatitis include serum calcium and triglyceride levels. (medscape.com)
Indirect Reference Intervals of Serum Calcium in Pakistani Children Using KOSMIC, Bhattacharya, and Hoffman Approach. (aku.edu)

Of the discrepant Farr test(+), IIF-CL(-) sera, slightly fewer than half corresponded to a false positive reaction of the Farr test due to the presence in the serum of proteins other than Ab which were able to bind to the labelled ds-DNA to form a complex precipitable by 50% saturated ammonium sulfate solution. (nih.gov)
This risk assessment was not dependent on the type of adju- decoding patterns of immunoregulatory serum proteins could re- vant therapy received by the patients. (lu.se)

The retrospective study was conducted at Bursa Uludag University Hospital, Bursa, Turkey, and comprised data from January 2009 to December 2017 related to patients of either gender with suspected cystic echinococcosis who underwent indirect haemagglutination testing. (org.pk)

Each serum sample was subjected to the three tests and the results obtained were recorded and the test outcomes were cross-classified to estimate the sensitivity and specificity of the tests using Bayesian model. (hindawi.com)

After analyzing this test in sera from selected groups of patients, the frequency of both specific IgG and IgM of immunocompetent children who were excreting oocysts in their faeces was 62% and in children with negative excretion of oocysts was 20% and 40%, respectively. (scielo.br)
For patients presenting with a broader phenotype concerning for immune-mediated movement disorder, consider ARUP's Autoimmune Movement Disorder Panel in serum ( 3006206 ) or CSF ( 3006207 ). (arupconsult.com)
Abstract Background Most studies focused on the benefits of lycopene on serum lipids but no studies have been specifically designed to assess the role of a tomato sauce from vine-ripened tomatoes on patients affected by polygenic hypercholesterolemia. (researchgate.net)
We tested the sera from 10 patients with NMO, 10 with MS and five with other neurological disorders. (go.jp)
Whereas in most normal adults serum levels of IgE were below 300 units/ml, raised levels of IgE were found in patients infected with helminths. (ajtmh.org)
However, a simple way to screened 240 sera from 64 patients with primary breast cancer. (lu.se)

has had a negative serum pregnancy test within 2 weeks prior to beginning therapy. (nih.gov)

In the study realized, by an indirect immunofluorescence technique, specific immunoglobulins (IgG and IgM) have been demonstrated in human serum against Cryptosporidium oocysts. (scielo.br)
Children infected with human immunodeficiency virus had no IgM and only 14% had IgG detectable in their sera. (scielo.br)

and total homocysteine in serum or plasma. (kcl.ac.uk)
Total iron-binding capacity (TIBC) is an indirect method assessment of transferrin levels. (medscape.com)

Among the remaining 798 sera (34.7% of positive sera), 48.25% gave a positive reaction with the Farr test and 51.75% with the IIF reaction. (nih.gov)
Slightly more than half of the other Farr test(+) IIF-C'(-) sera corresponded to a defect in the IIF-CL reaction. (nih.gov)

Methylmalonic acid quantification in low serum volumes by UPLC-MS/MS . J Chromatogr B 2011;879:1502-6. (kcl.ac.uk)
There was significant positive correlation between fasting and residual volumes and age, weight and height, and between fasting volume and body mass index and serum ferritin level. (who.int)

A relationship with Tibrogagan virus was demonstrated by serum neutralization and cross-indirect immunoflourescence. (cdc.gov)

But how do they compare to serum measurements? (aacc.org)

Twenty three percent of immunocompromised adults with not determined excretion of oocysts in their stools had anti-Cryptosporidium IgG in their sera. (scielo.br)

Evaluate abnormal serum liver tests with repeat testing within 3 to 5 days. (salesandmarketingnetwork.com)
If the abnormalities are confirmed, monitor serum liver tests weekly until resolved or stabilized. (salesandmarketingnetwork.com)

Today, pemphigus, pemphigoid, and psoriasis, which were of unknown etiology, are now diagnosed worldwide by immunofluorescent studies of blood serum and/or biopsies of skin or affected mucous membranes. (wikipedia.org)

Of the sera giving positive reactions, only 1,499 (65.3% of the positive sera) were positive with both reactions. (nih.gov)

Anatomy10

Organisms29

Diseases42

Chemicals and Drugs87

Analytical, Diagnostic and Therapeutic Techniques and Equipment57

Phenomena and Processes29

Disciplines and Occupations1

Information Science3

Named Groups2

Health Care8

Antibodies13

Bilirubin15

Antibody11

Biomarkers8

Assay4

ELISA1

Haptoglobin1

Measurement2

Protein5

Specimen2

Calorimetry3

Creatinine3

Titers1

Diagnosis1

Dilution2

Samples8

Acute1

Concentration4

Concentrations2

Evaluation2

Calcium2

Proteins2

Underwent1

Sensitivity1

Patients6

Pregnancy1

Human2

Total2

Test2

Volumes2

NEUTRALIZATION1

Measurements1

Adults1

Tests2

Blood1

Positive1