In the current study, we demonstrate for the first time that the over-expression of Rabin8, which functions as a guanine nucleotide exchange factor for Rab8 GTPase, suppresses phosphorylation of Ser235/Ser236 in ribosomal protein S6. (actanaturae.ru)
In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four conserved Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity. (nih.gov)
CK1-dependent phosphorylation of Ser-247 was induced by mitogenic stimuli and required prior phosphorylation of upstream S6 kinase/ribosomal S6 kinase residues. (nih.gov)
Additionally, we show that protein phosphatase 1 (PP1) antagonizes rpS6 C terminus phosphorylation and cap binding in intact cells. (nih.gov)
Studies using S6 knockin mice, where all five phosphorylation site serine residues are replaced by alanine, have provided extensive detail on S6 function. (abwizbio.com)
The phosphorylation sites are located in the carboxyl terminus of the protein and have been mapped in mammals to Ser-235, -236, -240, -244, and -247 (21). (pancreapedia.org)
In a variety of specialized cell types including muscle, neurons and secretory cells, S6 phosphorylation is temporally correlated to the initiation of protein synthesis. (pancreapedia.org)
Deletion of these genes greatly reduced S6 kinase activity but revealed that phosphorylation of rpS6 could be carried out in part by other kinases including Protein kinase A (PKA) (7), protein kinase C (PKC) (23), RSK (8), and casein Kinase 1 (15). (pancreapedia.org)
As TORC1 is activated by a variety of growth factors, hormones and nutrients (34), this explains the general correlation of rpS6 phosphorylation with protein synthesis and growth. (pancreapedia.org)
Subunit5
BACKGROUND Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. (sputnic-group.ru)
Ribosomal protein S6 is a cytoplasmic ribosomal protein that is a component of the 40S subunit. (sputnic-group.ru)
Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5'-m(7)GpppG cap of mRNA. (nih.gov)
Ribosomal protein S6 (rpS6) is one of 33 proteins which along with 18S ribosomal RNA make up the small (40S) subunit of the eukaryotic ribosome. (pancreapedia.org)
It is located in the small head region of the 40S subunit and resides at the interface of the 40S and 60S (large) subunits where a groove is thought to be the site of new protein synthesis (35). (pancreapedia.org)
Kinase5
2 Once p70S6K is activated, it acts on downstream a number of effectors including 40S ribosomal protein S6 , protein synthesis initiation factor 4B, and elongation factor 2 kinase. (sputnic-group.ru)
In this study, we employed phospho-specific antibodies to show that Ser-247 is a target of the casein kinase 1 (CK1) family of protein kinases. (nih.gov)
Ribosomal protein S6 kinase is one of two parallel signaling pathways downstream of mTOR, with the other being 4E-BP1. (abwizbio.com)
Avian and mammalian cells were then shown to also contain a distinct 70 kDa S6 kinase referred to as p70 S6K but now known simply as S6K which can phosphorylate all five sites on rpS6 (1, 18). (pancreapedia.org)
Antibody1
Flow cytometric analysis of K562 cells, untreated and unstained as negative control (blue) or untreated (red) or treated with EGF (green), and stained using Phospho-S6 ribosomal protein (Ser240/Ser244) antibody S6S240S244-CD10 APC conjugate Cat. (abwizbio.com)
Detects endogenous Phospho-RPS6 (Ser235/Ser236) without cross-reactivity with other family members. (sputnic-group.ru)
rpS6 is an evolutionary conserved protein of 236 to 253 amino acids and is the first discovered and main phosphorylated ribosomal protein as shown originally by Gressner and Wool (13). (pancreapedia.org)
Dephosphorylation of rpS6 is carried out primarily by a protein phosphatase 1 (15). (pancreapedia.org)
Phosphorylates1
Activated PKB phosphorylates and subsequently inactivates TSC2, a GTPase-activating protein, leading to an increase in GTP bound Rheb. (sputnic-group.ru)
Synthesis2
These studies support the role phosphorylated S6 plays in regulation of cell size, glucose homeostasis, and protein synthesis. (abwizbio.com)
Most notably, replacing all five phosphorylatable serines in S6 with alanine failed to inhibit protein synthesis in MEFs derived from the knock in mice (27). (pancreapedia.org)
Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. (sputnic-group.ru)
Apoptosis2
Deregulated miR-155 escalates the appearance degree of Fas-associated loss of life domain-containing proteins (FADD) and caspase-3, marketing Fas-mediated apoptosis in IDD (27). (rawveronica.com)
Up-regulated appearance of enhancer-like lncRNA RP11-296A18.3 was observed, causing the overexpression of Fas-associated proteins aspect-1 which induces the Fas-mediated apoptosis of NP cells finally. (rawveronica.com)
MRNA2
The ribosomal protein S6 may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. (sputnic-group.ru)
These particular mRNA transcripts (5'TOP) contain an oligopyrimidine tract in their 5' untranslated regions and encode components of the translational machinery and some proteins involved in cell cycle progression. (sputnic-group.ru)