Sequential mammalian embryos. Medical search. Frequent questions

Determine PIF levels in culture medium of multiple mouse and single bovine embryos cultured up to the blastocyst stage using PIF-ELISA. (biomedcentral.com)
Giraldo A.M., Hylan D.A., Ballard C.B., Purpera M.N., Vaught T.D., Lynn J.W., Godke R.A., Bondioli K.R., Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos. (biologie-journal.org)

This system drives the rhythmic, sequential, and precise formation of embryonic body segments, exhibiting rich spatial and temporal phenomena spanning from molecular to tissue scales. (ens-lyon.fr)
Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. (caltech.edu)
Modeling human embryo development with embryonic and extra-embryonic stem cells. (caltech.edu)
We explore the method on three circuits for haematopoiesis and embryonic stem cell development for commitment and reprogramming scenarios and illustrate how the method can be used to determine sequential steps for onsets of external factors, essential for efficient reprogramming. (lu.se)
Christians E., Rao V.H., Renard J.P., Sequential acquisition of transcriptional control during early embryonic development in the rabbit. (biologie-journal.org)
Several investigations [ 5 - 7 ] have been carried out with isolated embryonic, fetal, and adult SCs in a well-defined culture microenvironment to define the sequential steps and intracellular pathways that are involved in their differentiation into the specific cell lineages. (biomedcentral.com)
It is concerned with the sequential stages of embryonic and fetal development, beginning with fertilization. (ac.ir)
Furthermore, due to ethical and legal concerns, very limited techniques can be applied to human embryos to validate some of significant conclusions drawn from descriptive studies regarding human embryonic development. (nature.com)

Together, this comprehensive delineation of mammalian cell differentiation trajectories in vivo represents a baseline for understanding the effects of gene mutations during development, as well as a roadmap for the optimization of in vitro differentiation protocols for regenerative medicine. (nih.gov)
Machine learning-assisted high-content analysis of pluripotent stem cell-derived embryos in vitro. (caltech.edu)
Huang J.C., Lei Z.L., Shi L.H., Miao Y.L., Yang J.W., Ouyang Y.C., Sun Q.Y., Chen D.Y., Comparison of histone modifications in in vivo and in vitro fertilization mouse embryos. (biologie-journal.org)

Rossant, J. & Vijh, K. M. Ability of outside cells from preimplantation mouse embryos to form inner cell mass derivatives. (nature.com)
Aoki F., Worrad D.M., Schultz R.M., Regulation of transcriptional activity during the first , and second cell cycles in the preimplantation mouse embryo. (biologie-journal.org)

In Drosophila , the BX-C cluster contains the three most posterior Hox genes, where their collinear activation incorporates progressive replacement of histone modifications, reorganization of 3D chromatin architecture and sequential activation of boundary elements and cis -regulatory regions. (biorxiv.org)
Hox genes encode crucial developmental regulators that specify segmental identities along the Antero-Posterior (A-P) body axis in the developing embryo of bilaterian species. (biorxiv.org)
These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. (uth.edu)
Unlike the other E(spl)-C genes, the gene is expressed within neuronal cells in the embryo. (sdbonline.org)
Expression analysis of several Hox genes in human embryos. (cnr.it)
Three hemoglobin genes are specifically expressed in the embryo, four genes in the filter feeding larva (ammocoete), and nine genes correspond to the adult hemoglobin chains. (biomedcentral.com)

Here we report the transcriptional profiles of 116,312 single cells from mouse embryos collected at nine sequential time points ranging from 6.5 to 8.5 days post-fertilization. (nih.gov)
Furthermore, we use single-cell profiling to show that Tal1 -/- chimeric embryos display defects in early mesoderm diversification, and we thus demonstrate how combining temporal and transcriptional information can illuminate gene function. (nih.gov)
Mammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. (uth.edu)
This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation, and metabolism in a step-wise fashion from cleavage to morula. (uth.edu)
Adenot P.G., Mercier Y., Renard J.P., Thompson E.M., Differential H4 acetylation of paternal, maternal chromatin precedes DNA replication, and differential transcriptional activity in pronuclei of 1-cell mouse embryos. (biologie-journal.org)

Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos. (uth.edu)
His research has led to an atlas of gene expression that has the potential to revolutionize the understanding of mammalian development with attendant clinical implications for birth defects and other developmental disorders. (washington.edu)
Bellier S., Chastant S., Adenot P., Vincent M., Renard J.P., Bensaude O., Nuclear translocation , and carboxyl-terminal domain phosphorylation of RNA polymerase II delineate the two phases of zygotic gene activation in mammalian embryos. (biologie-journal.org)
Isolation and expression analysis both in invertebrate and vertebrate embryos of a new homeobox containing gene named Orthopedia (Otp). (cnr.it)
Isolation and expression analysis in murine embryos of Dlx5 and Dlx6, two murine homologues of the Drosophila Distal-less gene. (cnr.it)

A model of the post-implantation human embryo derived from pluripotent stem cells. (caltech.edu)
Fulka H., St John J.C., Fulka J., Hozak P., Chromatin in early mammalian embryos: achieving the pluripotent state. (biologie-journal.org)

Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. (nature.com)

Driever, W. & Nüsslein-Volhard, C. A gradient of bicoid protein in Drosophila embryos. (nature.com)
Driever, W. & Nüsslein-Volhard, C. The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner. (nature.com)

The mammalian telencephalon, which comprises the cerebral cortex, olfactory bulb, hippocampus, basal ganglia (striatum and globus pallidum), and amygdala, is a highly complex and evolutionarily advanced brain structure. (intechopen.com)
We trained adult zebrafish in an appetitive odor discrimination task and analyzed odor representations in a specific compartment of the posterior zone of the dorsal telencephalon (Dp), the homolog of mammalian olfactory cortex. (janelia.org)

In single bovine IVF-derived embryos, PIF levels in medium at day 3 of culture were higher than non-cleaving embryos (control) (P = 0.01) and at day 7 were higher than day 3 (P = 0.03). (biomedcentral.com)

Louvet-Vallee, S., Vinot, S. & Maro, B. Mitotic spindles and cleavage planes are oriented randomly in the two-cell mouse embryo. (nature.com)
Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)
This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
We conclude that cel -free DNA levels in CM might be associated with delayed embryo cleavage. (who.int)

Here, we present whole-genome chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) analyses of a wide variety of histone markers in the brain, heart, and liver of early human embryos shortly after their formation. (researchgate.net)

Powerful advancements in molecular genetic manipulation and assisted reproductive technolo-gies are employed during embryo and fetal development, and these efforts have had profound impact on animal production worldwide. (ac.ir)

Adenot P.G., Szollosi M.S., Geze M., Renard J.P., Debey P., Dynamics of paternal chromatin changes in live one-cell mouse embryo after natural fertilization. (biologie-journal.org)

Despite the importance of the dermis in the structural and functional integrity of the skin, genetic analysis of dermal development in different parts of the embryo is incomplete. (biologists.com)
The signaling requirements for ventral dermal cell development have not been established in either the chick or the mammalian embryo. (biologists.com)
The mechanisms underlying the appearance of asymmetry between cells in the early embryo and consequently the specification of distinct cell lineages during mammalian development remain elusive. (nature.com)
Figure 2: The self-organization theory of early mammalian development. (nature.com)
To determine the role of PIF in post-fertilization embryo development, we measured the peptide's concentration in the culture medium and tested endogenous PIF's potential trophic effects and direct interaction with the embryo. (biomedcentral.com)
Examine the inhibitory effects of anti-PIF-monoclonal antibody (mAb) added to medium on cultured mouse embryos development. (biomedcentral.com)
PIF is an early embryo viability marker that has a direct supportive role on embryo development in culture. (biomedcentral.com)
Overall, our data supports PIF's endogenous self sustaining role in embryo development and the utility of PIF- ELISA to detect viable embryos in a non-invasive manner. (biomedcentral.com)
Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. (uth.edu)
Sequential expression of multiple POU proteins during amphibian early development. (wikigenes.org)
After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
[ 2 ] This hypothesis detailed the sequential development of junctional, compound, and finally purely dermal melanocytic nevi. (medscape.com)

PIF levels in mouse embryo culture medium significantly increased from the morula to the blastocyst stage (ANOVA, P = 0.01). (biomedcentral.com)

In collaboration with the laboratories of Drs. Jay Shendure in Genome Sciences and Kelly Stevens in Bioengineering, his research has focused on developing high throughput single cell RNA sequencing technologies, employing "sequential combinatorial indexing. (washington.edu)
Abdalla H., Yoshizawa Y., Hochi S., Active demethylation of paternal genome in mammalian zygotes. (biologie-journal.org)
Although the genome-wide DNA demethylation is believed to be a hallmark of mammalian embryogenesis, previous study also indicated that the somatic form of dnmt1 ( dnmt1s ) is actually expressed at each stage of pre-implantation embryos and plays a role in the maintenance of DNA imprinting 8 . (nature.com)
As part of the genome sequencing initiative for the tammar, we have explored the evolution of each of the major classes of mammalian small RNAs in an Australian marsupial for the first time, including the first genome-scale analysis of the newest class of small RNAs, centromere repeat associated short interacting RNAs (crasiRNAs). (biomedcentral.com)

The invention provides transgenic non-human animals, such as transgenic rodents and transgenic non-human mammalian cells harboring a transgene encoding an APP polypeptide comprising the Swedish mutation. (justia.com)
Low-quality embryos, on the other cells, thereby maintaining a relatively low basal hand, frequently display morphological level [16-18]. (who.int)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
Three isoforms have been described in mammalian cells. (lookformedical.com)
Most recombinant proteins are currently produced in prokaryotic cells (mainly the bacterium Escherichia coli ) and a small number of well-characterized mammalian cell lines, such as Chinese hamster ovary (CHO) cells. (frontiersin.org)
It is now established that melanocytic nevus precursor cells are derived from neural crest tissue and migrate to and populate the developing epidermis of the embryo. (medscape.com)

A strong and high-shifting binding activity was detected in the extracts of zebrafish embryo (12 h after fertilization), and the expression of the activity dramatically decreased in 60 to 84-h-old zebrafish. (ncl.edu.tw)
Haaf T., The battle of the sexes after fertilization: behaviour of paternal and maternal chromosomes in the early mammalian embryo. (biologie-journal.org)

More recently, he has developed a new method for sequencing mRNA transcripts at near single cell resolution in developing embryos. (washington.edu)

Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. (uth.edu)
In 1-cell and 2-cell embryos Dnmt1s is derived from the oocyte, whereas from the 2-cell stage onward the embryo starts to synthesize its own Dnmt1s 8 . (nature.com)

The role of polarization and early heterogeneities in the mammalian first cell fate decision. (caltech.edu)

Human embryo polarization requires PLC signaling to mediate trophectoderm specification. (caltech.edu)

A pathway for taurine biosynthesis from serine and sulfate is reported in microalgae, developing chicken embryos, and chick liver. (wikipedia.org)

Some anatomical structures in the developing embryo disappear completely or regress substantially once they serve their developmental purpose, remaining only as scar-like vestiges (literally, "footprints") in the mature human. (answersingenesis.org)

Since the immune milieu of pregnancy is unique - not replicated in any other circumstances - specific embryo-derived signals have a crucial role leading to maternal recognition of pregnancy [ 4 ]. (biomedcentral.com)

To orchestrate such critical 'cross-talk', a viable embryo must be present, which may be accepted by the mother, whereas, a non-viable conceptus will fail to develop or later be rejected since maternal acceptance does not occur. (biomedcentral.com)

Embryos and fetuses were extracted via a ventral incision on the abdominal and uterine walls of one pregnant doe each day after euthanization with thiopental sodium at the dose of 31 mg/kg body weight intravenously. (ac.ir)

Figure 1: Classic models for lineage segregation in the early mouse embryo. (nature.com)

Generation of Stem Cell-Based Mouse Embryo-Like Structures. (caltech.edu)
UV-binding factors found in 12-h-old zebrafish embryos may be involved in processing developmental stage-specific DNA structures similar to UV-damage DNA. (ncl.edu.tw)

Haeckel produced these artistic drawings, supposedly based on his own specimens 1 of different embryos, claiming that all of them pass through stages reminiscent of their evolutionary ancestors. (answersingenesis.org)

Mammalian taurine synthesis occurs in the pancreas via the cysteine sulfinic acid pathway. (wikipedia.org)
Bouniol C., Nguyen E., Debey P., Endogenous transcription occurs at the 1-cell stage in the mouse embryo. (biologie-journal.org)

Kopecny V., Fléchon J.E., Camous S., Fulka J. Jr., Nucleologenesis , and the onset of transcription in the eight-cell bovine embryo: fine-structural autoradiographic study. (biologie-journal.org)

Do human embryos replay the evolutionary history of their species as they develop? (answersingenesis.org)
Do developing embryos really replay the evolutionary history of their species as they develop? (answersingenesis.org)

The group studies a population of coupled genetic oscillators in the vertebrate embryo termed the segmentation clock. (ens-lyon.fr)

Stem-cell-based human and mouse embryo models. (caltech.edu)

However, the market is still dominated by prokaryotic and mammalian expression systems, the former offering high production capacity at a low cost, and the latter favored for the production of complex biopharmaceutical products. (frontiersin.org)

Detectable - though low - PIF levels were secreted already by 2-cell stage mouse embryos. (biomedcentral.com)

The binding activity expressed in embryos was apparently unrelated to human DNA repair damage-recognition proteins XPA、RPA-70 and RPA-32, since those polypeptides recognized by an anti-human XPA、RPA-70 and RPA-32 antibody were detected only in 84-h-old zebrafish extracts. (ncl.edu.tw)

Identification and isolation of damage-recognition protein complexes from zebrafish(Danio rerio) early embryos. (ncl.edu.tw)

Piotrowska, K. & Zernicka-Goetz, M. Role for sperm in spatial patterning of the early mouse embryo. (nature.com)

In non-cleaving embryos culture medium was similar to medium alone (control). (biomedcentral.com)
Measurement of free radicals in the culture medium of developing embryos has been reported as an index for embryo viability. (biomedcentral.com)

PIF-ELISA use to assess IVF embryo quality prior to transfer is warranted. (biomedcentral.com)
Due to its clinical applications success of IVF results is the morphological and the expansion of non-invasive treatment quality of the embryo. (who.int)

Anatomy53

Organisms30

Diseases4

Chemicals and Drugs47

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Phenomena and Processes79

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science5

Health Care1

Bovine embryos2

Embryonic8

Vitro3

Preimplantation mouse2

Genes6

Transcriptional5

Gene5

Pluripotent2

Methylation1

Drosophila2

Telencephalon2

0.031

Cleavage4

Histone1

Fetal1

Chromatin1

Development13

Blastocyst1

Genome4

Cells6

Fertilization2

MRNA1

Oocyte2

Fate1

Specification1

Chick1

Anatomical1

Crucial1

Maternal1

Ventral1

Lineage1

Structures2

Stages1

Occurs2

Transcription1

Species2

Genetic1

Human and m1

Expression1

Stage1

Unrelated to human1

Protein1

Early mouse1

Culture2

Quality2