Sequencing genome mitochondrial. Medical search. Frequent questions

The sources of genome expansion include group I introns and accessory genes encoding putative homing endonucleases, DNA and RNA polymerases (presumed to be of plasmid origin) and hypothetical proteins. (umd.edu)
The two smallest sequenced genomes (A. terreus and P. chrysogenum) do not contain introns in protein-coding genes, whereas the largest genome (T. stipitatus), contains a total of eleven introns. (umd.edu)
Despite the conservation of the core genes, the mitochondrial genomes of Aspergillus and Penicillium species examined here exhibit significant amount of interspecies variation. (umd.edu)
Most of this variation can be attributed to accessory genes and mobile introns, presumably acquired by horizontal gene transfer of mitochondrial plasmids and intron homing. (umd.edu)
We sequenced the complete mt genome of W. compar and conducted phylogenetic analyses using concatenated coding sequences of 12 protein-coding genes (PCGs) by maximum likelihood and Bayesian inference. (nature.com)
The complete mt genome is 14,373 bp in size and comprises 36 genes, including 12 protein-coding, two rRNA and 22 tRNA genes. (nature.com)
However, recent molecular studies based on two mitochondrial genes found that the lineage is deeply embedded within the scosp-owl Otus clade rather than with the much larger Eagle-owl Bubo (Miranda et al 1997, Miranda et al 2010). (emerging-researchers.org)
We find frequent somatic nuclear transfers of mitochondrial DNA, some of which disrupt therapeutic target genes. (lu.se)
Co-expression analysis highlights the function of mitochondrial genes in oxidative phosphorylation, DNA repair and the cell cycle, and shows their connections with clinically actionable genes. (lu.se)
Surprisingly, both of our thylacine sequences differ by 11%-15% from putative thylacine mitochondrial genes in GenBank, with one of our samples originating from a direct offspring of the previously sequenced individual. (psu.edu)
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. (wikipedia.org)
This first comprehensive analysis includes genes and proteins and their relation to human disease, repeated sequences, comparative genome-wide studies of mammalian orthologous chromosomal regions and rearrangement breakpoints, reconstruction of ancestral karyotypes and the events leading to existing species, rates of variation, and lineage-specific and lineage-independent evolutionary events such as expansion of gene families, orthology relations and protein evolution. (nih.gov)
The cp genome of A. tsao-ko has 120 annotated genes , including 82 protein - coding genes , while A. paratsaoko has 121 annotated genes , including 83 protein - coding genes . (bvsalud.org)
Both cp genomes contained 30 tRNA genes and 8 rRNA genes . (bvsalud.org)
The S. purpurea mt genome contains 52 genes: 31 protein-coding, 18 tRNAs, and three rRNAs. (springeropen.com)
First, all hitherto known ciliate mitochondrial genomes are linear, from 40 kb ( P. tetraurelia ) to 47 kb ( Tetrahymena ) long, and contain some 50 tightly packed genes without introns. (biomedcentral.com)
Three rRNA genes, 17 tRNA genes and five pseudo tRNA sequences were identified. (bioseek.eu)
In addition, eleven ribosomal protein genes and two pseudo ribosomal protein genes were found, which are homologous to 13 of the 16 genes for ribosomal proteins in the mitochondrial genome of the liverwort (Marchantia polymorpha). (bioseek.eu)
There are 16,569 base pairs of mitochondrial DNA which only code for 37 genes. (bartleby.com)
RNA sequencing allows scientists to study the expression of genes in a cell. (epfl.ch)
Since messenger RNA (mRNA) is generated from a DNA gene, that information can be used to identify the original gene sequence and thus measure the activity of thousands of genes (i.e. the transcriptome) in these cells. (epfl.ch)
Watkins PA, Maiguel D, Jia Z, Pevsner J. Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome. (medlineplus.gov)
In addition, mitochondrial DNA (mtDNA) encodes 2 ribosomal RNA genes and 22 transfer RNA (tRNA) genes necessary for the intramitochondrial synthesis of these 13 polypeptides. (medscape.com)
A deletion anywhere in the mitochondrial genome may affect transcription or translation of genes that were not affected by the deletion. (medscape.com)

Silver Age of GOLD Introduces New Features The Genomes OnLine Database makes curated microbiome metadata that follows community standards freely available and enables large-scale comparative genomics analysis initiatives. (doe.gov)
This research will contribute to protecting New Zealand's natural heritage, build capacity for genome scale projects in New Zealand, and help establish New Zealand at the cutting edge of the rapidly growing field of conservation genomics. (otago.ac.nz)
The high-throughput - next generation sequencing (HT-NGS) technologies are currently the hottest topic in the field of human and animals genomics researches, which can produce over 100 times more data compared to the most sophisticated capillary sequencers based on the Sanger method. (springer.com)
The leading genome research centers and scientists have publicly recognized that these are the core enabling goals for the next decade genomics research. (springer.com)
The National Human Genome Research Institute (NHGRI) has echoed this need through its vision for genomics research (Collins et al. (springer.com)
As the integration of NGS in the study and treatment of cancer continues to mature, we believe that the field of cancer genomics will need to move toward more complete 100% genome sequencing. (frontiersin.org)
First, this article summarizes the history of massively parallel next-generation sequencing (NGS) in the context of cancer genomics and reviews recent research and clinical applications. (frontiersin.org)
are applicable to biobanks associated with genome, and the development of genomics population-based studies coordinated by databases and new bioinformatics tools. (who.int)

As a first step in evaluating the most variable molecular markers for further population genetic and conservation studies of this and other insects, the entire mitochondrial genome (mitogenome) was sequenced (16,094bp). (bioone.org)
The entire mitochondrial genome of rice (Oryza sativa L.), a monocot plant, has been sequenced. (bioseek.eu)

While whole exome sequencing (WES) remains popular and effective as a method of genetically profiling different cancers, advances in sequencing technology has enabled an increasing number of whole-genome based studies. (frontiersin.org)
Compared to whole genome sequencing (WGS), exome sequencing covers only the 1% of the genome that is translated into protein, greatly reducing the technical burden of data collection and analysis. (frontiersin.org)
Alfares A, Nunez LD, Al-Thihli K, Mitchell J, Melancon S, Anastasio N, Ha KC, Majewski J, Rosenblatt DS, Braverman N. Combined malonic and methylmalonic aciduria: exome sequencing reveals mutations in the ACSF3 gene in patients with a non-classic phenotype. (medlineplus.gov)
Exome sequencing identifies ACSF3 as a cause of combined malonic and methylmalonic aciduria. (medlineplus.gov)
This technology involves breaking the entire genome into small segments, sequencing the segments, and then reassembling the sequences using intensive computational techniques to provide the base-by-base sequence of the entire genome or more limited regions, such as the expressed portion of the genome known as the exome. (msdmanuals.com)
However, the sheer volume of information generated by sequencing the exome or genome results in a variety of interpretive problems that complicate understanding of the results. (msdmanuals.com)
Multiple genome-wide association and whole exome sequencing studies have provided extraordinary insights into the genetic variants underlying familial as well as polygenic forms of the disease. (lu.se)

Li-Wei Wu , David C. Lees , and Shen-Horn Yen "The Complete Mitochondrial Genome of the Near-Threatened Swallowtail, Agehana maraho (Lepidoptera: Papilionidae): Evaluating Sequence Variability and Suitable Markers for Conservation Genetic Studies," Entomological News 121(3), 267-280, (1 June 2010). (bioone.org)
To characterize genetic diversity and gene flow among Daphnia galeata populations, the complete nucleotide (nt) sequences of the mitochondrial (mt) DNAs of D. galeata clones isolated from five lakes in Japan (Lakes Shirakaba, Suwa, Kizaki, Kasumigaura, and Biwa) were determined. (unboundmedicine.com)
The study expands the genomic resources available to fungal biologists by providing mitochondrial genomes with consistent annotations for future genetic, evolutionary and population studies. (umd.edu)
Our two sequences differ in only five nucleotides out of 15,452, hinting at a very low genetic diversity shortly before extinction. (psu.edu)
The secondary PCR products were sequenced in both directions on an ABI 3130 Genetic Analyzer (Applied Biosystems). (cdc.gov)
A neighbor-joining analysis was used to assess the genetic relatedness of various C. cayetanensis sequences for each locus and concatenated sequences of 5 loci. (cdc.gov)
Although several studies have investigated genetic diversity of Leishmania infantum in North Africa, genome-wide analyses are lacking. (hal.science)
The results indicate frequent and independent DNA sequence flow to and from the mitochondrial genome during the evolution of flowering plants, and this may account for the range of genetic variation observed between the mitochondrial genomes of higher plants. (bioseek.eu)
Identification of genetic risk loci and causal insights associated with Parkinson's disease in African and African admixed populations: a genome-wide association study. (ucl.ac.uk)
Massively parallel sequencing enables the exploration of the genetic heterogeneity within microbial, viral and tumor cell populations. (avhandlingar.se)
Instead of using random and often neutral genetic markers to guide conservation efforts, complete genome data will be used to evaluate how populations of threatened bird species differ on a functional genomic level and to develop targeted conservation strategies based on these data. (otago.ac.nz)
For the future direction, we need the in-depth genome sequence information and analysis for most of the mammals, including human to fully understand genome variation of economic traits, genetic susceptibility to diseases, and pharmacogenomics of drug response. (springer.com)
Until recent years, the mitochondrial genome, located in the mitochondrion, and the genetic information encoded by it have been given little attention. (bartleby.com)
Nuclear DNA harbors most of the genetic information, while the much smaller mitochondrial genome is present in thousands of copies. (nobelprize.org)
The mitochondrial genome is small and contains only a fraction of the genetic information in the cell, but it is present in thousands of copies, increasing the chance of success. (nobelprize.org)
The mitochondrial genome encodes the genetic information for the 13 polypeptide subunits essential for the process of oxidative phosphorylation. (medscape.com)
Next-generation sequencing technologies have dramatically changed the approach to genetic diagnosis. (msdmanuals.com)
This revolutionary and rapidly evolving technology has moved a significant portion of the technical aspects of genetic diagnosis to next-generation sequencing and has become the mainstay of genetic diagnosis. (msdmanuals.com)

The mitochondrial genome refers to all the DNA in the mitochondrion, the energy-producing component of most eukaryotic (complex) cells. (sciencedaily.com)

Mitochondrial diseases are a heterogeneous group of disorders that can be caused by mutations in the nuclear or mitochondrial genome. (prinsesmaximacentrum.nl)
Next-generation sequencing (NGS) techniques have led to major breakthroughs in the characterization of the chronic lymphocytic leukemia (CLL) genome with discovery of recurrent mutations of potential prognostic and/or predictive relevance. (avhandlingar.se)
However, recently it became apparent that the mitochondrial genome, despite its small size, is crucial for the study of human evolution and disease, as mtDNA mutations lead to some serious diseases. (bartleby.com)
Certain mitochondrial DNA mutations have been found to result in mitochondrial dysfunction and have been found to be heavily implicated in the aging process as well as various age-related disorders and diseases. (bartleby.com)

Their mitochondrial genomic sequences may hold vital clues into the mechanisms of their evolution, population genetics, and biology, yet only a handful of these genomes have been fully sequenced and annotated. (umd.edu)
Our data sample each mitochondrial nucleotide an average of 50 times, thereby providing the first high-fidelity reference sequence for thylacine population genetics. (psu.edu)
Our bioinformatics pipeline more accurately captures features of mitochondrial genetics than existing pipelines that are important in understanding how mitochondrial dysfunction contributes to disease. (prinsesmaximacentrum.nl)

All of the sequenced genomes have a group I intron in the large ribosomal subunit RNA gene, suggesting that this intron is fixed in these species. (umd.edu)
Methodology/Principal findings To understand the species' population relationships and dispersal patterns within Europe, a fragment of the cytochrome oxidase I (COI or COX1) gene was sequenced from 130 mosquitoes, collected from five countries where the species has been introduced and/or established. (fmach.it)
39S ribosomal protein L4, mitochondrial is a protein that in humans is encoded by the MRPL4 gene. (wikipedia.org)
Plant mitochondrial (mt) genomes possess several complex features, including a variable size, a dynamic genome structure, and complicated patterns of gene loss and gain throughout evolutionary history. (springeropen.com)
The analysis of mitochondrial and nuclear gene sequences supports all studied tumours belong to a single neoplastic lineage that spreads in the Seas of Southern Europe. (sciety.org)
Regarding mapping of reads to different reference Cox1 sequences (for Figure 2a): "Then, we mapped the paired-end reads onto a dataset containing non-redundant mitochondrial Cytochrome C Oxidase subunit 1 (Cox1) gene references from 137 Vererid clam species. (sciety.org)
To test this question, we determined the mitochondrial genome sequence of Paramecium caudatum and compared the gene content and sequence properties to the closely related P. tetraurelia . (biomedcentral.com)
This gene is ~1,350 amino acid residues long, shows extreme sequence divergence among Tetrahymena species and has no homolog in the P. tetraurelia or Euplotes mitochondrial genomes. (biomedcentral.com)
We found that the apicoplast genome of this parasite consists of a 34?kb monocistronic circular molecule encoding functions that are important for apicoplast gene transcription as well as translation and maturation of the organelle's proteins. (pacb.com)

Oxford Nanopore and Illumina sequencing techniques were combined to generate the first complete nuclear and mitochondrial genomic sequences of Ae. (fmach.it)

Accurate mtDNA mapping from WGBS data required alignment to an mtDNA reference only, to avoid misalignment to nuclear mitochondrial pseudogenes. (sens.org)
In addition, we propose a robust framework for methylation analysis of mtDNA from WGBS data, which is less prone to false-positive findings due to misalignment of nuclear mitochondrial pseudogene sequences. (sens.org)
I want to detect NUMTS (nuclear mitochondrial sequences) that have migrated from the mitochondrial to the nuclear genome using the whole genome sequence data. (opensourcebiology.eu)

Currently, I am trying to align the raw sequenced data with the nuclear reference genome followed by the command line BLAST where I use the mitochondrial genome as Query against the database of nuclear reference genome of the same species. (opensourcebiology.eu)
Nuclear genome diversity of the isolates was analysed by comparison to the L. infantum JPCM5 reference genome. (hal.science)
DNA from a patient is compared to a reference genome using many oligonucleotide probes. (msdmanuals.com)

Sequence analysis identified alternatively spliced variants that encode different protein isoforms. (wikipedia.org)
Mitochondrial DNA (mtDNA) variants may exist in a state of heteroplasmy, where a percentage of DNA molecules harbor a variant, or homoplasmy, where all DNA molecules have the same variant. (prinsesmaximacentrum.nl)
To facilitate accurate identification of heteroplasmy and quantify mtDNA-CN, we built a bioinformatics pipeline that takes whole genome sequencing data and outputs mitochondrial variants, and mtDNA-CN. (prinsesmaximacentrum.nl)
During this period, tremendous success has been achieved in the fields of decoding of human genome, technological advancement of new era of human genome applications, toward personalized genomes and discovery of rare variants, leveraging genome sequencing to impact on cancer researches and mammalian evolution and population structure. (springer.com)
DNA microarrays can be used in genome-wide association studies (GWAS) to compare patient and control populations to identify DNA variants that can contribute to disease risk. (msdmanuals.com)

Here we report the complete sequence and annotation of the mitochondrial genomes of six Aspergillus and three Penicillium species: A. fumigatus, A. clavatus, A. oryzae, A. flavus, Neosartorya fischeri (A. fischerianus), A. terreus, P. chrysogenum, P. marneffei, and Talaromyces stipitatus (P. stipitatum). (umd.edu)
Investigators around the world are currently sequencing the complete mitochondrial genome of other birds but none has worked on many rare species such as the Giant Scops-Owl. (emerging-researchers.org)
The first complete genomic sequence also provides a significant leap in the general understanding of this species, opening the possibility for future genome-related studies, such as the detection of 'Single Nucleotide Polymorphism' markers. (fmach.it)
Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. (wikipedia.org)
Phylogenetic analysis using a total chloroplast genome DNA sequence of 28 species revealed a close relationship between A. tsao-ko and A. paratsaoko with 100% bootstrap value. (bvsalud.org)
Our analysis contributes toward understanding the organization and evolution of organelle genomes in Salicaceae species. (springeropen.com)
2015 ). Plant mt genomes can be extraordinarily larger than animal mt genomes, and vary significantly in size, even between very closely related species or within a single family (Alverson et al. (springeropen.com)
Remarkably, based on the analysis of mitochondrial DNA, the transmissible cancer seems to have jumped species. (sciety.org)
Mitochondrial genome sequencing analysis in tumour tissues from neoplastic animals revealed the coexistence of haplotypes from two different clam species. (sciety.org)
Phylogenies estimated from mitochondrial and nuclear markers confirmed this leukemia originated in striped venus clams and later transmitted to clams of the species warty venus, in which it survives as a contagious cancer. (sciety.org)
They then use Illumina sequencing to discover that the samples with cancer DNA had reads which mapped to the mtDNA reference sequences from a different clam species in the same family, suggesting a cross-species transmissible cancer. (sciety.org)
Earlier studies documented that the mitochondrial guanine + cytosine contents are very different between Paramecium tetraurelia and all studied Tetrahymena species. (biomedcentral.com)
This difference in the mitochondrial nucleotide composition was accompanied by significantly different codon usage patterns in both species, i.e. within P. caudatum clearly A/T ending codons dominated, whereas for P. tetraurelia the synonymous codons were more balanced with a higher number of G/C ending codons. (biomedcentral.com)
Therefore, to fill in one of the above mentioned gaps, we decided to sequence the mitochondrial genome of P. caudatum , a close relative of the P. aurelia species complex, in order to elucidate the mitochondrial genome evolution within this important ciliate genus. (biomedcentral.com)
Sequencing of the human genome and the genome of many other species has recently been completed and these data are available in the public domain to all researchers for interpretation. (icr.org)
To conduct their analyses, the researchers used a variety of techniques including isotope analyses, high-throughput genomic sequencing, bioinformatics, and phylogenetic analysis, which traces evolutionary relationships among species. (sciencedaily.com)

A rapid diagnostic 'mulitplex' one-step polymerase chain reaction protocol (RD-PCR) was developed using cytochrome oxidase subunit 1 (COX1) mitochondrial DNA (mtDNA) to discriminate between S. haematobium and S. bovis. (imperial.ac.uk)

The accompanying comparative analysis of these and related publicly available mitochondrial genomes reveals wide variation in size (25-36 Kb) among these closely related fungi. (umd.edu)
Lindqvist explains that ancient DNA studies have tended to focus on the mitochondrial genome because it generally reveals characteristics useful for evolutionary analyses and allows for DNA to be retrieved from ancient samples most easily. (sciencedaily.com)

Here, as part of the International Cancer Genome Consortium/The Cancer Genome Atlas Pan-Cancer Analysis of Whole Genomes Consortium, which aggregated whole-genome sequencing data from 2,658 cancers across 38 tumor types, we performed a multidimensional, integrated characterization of mitochondrial genomes and related RNA sequencing data. (lu.se)

Karlyshev, Andrey V. (2019) Remarkable features of mitochondrial DNA of 'Acanthamoeba polyphaga' Linc Ap-1, revealed by whole-genome sequencing. (kingston.ac.uk)

Background: Mitochondria contain small genomes that are physically separate from those of nuclei. (doe.gov)
Mitochondrial DNA is the small circular chromosome that is found inside mitochondria. (bartleby.com)
Mitochondria and Mitochondrial DNA are only passed from mother to offspring through the egg. (bartleby.com)
The DNA from the mitochondria is called the mitochondrial DNA (mtDNA), this DNA has over 16,000 base pairs (bps) that can be used to view the the lineage of a person's history. (bartleby.com)
The ACSF3 enzyme is found only in mitochondria and is involved in mitochondrial fatty acid synthesis. (medlineplus.gov)

The fossil's DNA is, by far, the oldest mammal mitochondrial genome to be sequenced -- about twice the age of the oldest genome sequence from a woolly mammoth. (sciencedaily.com)
Using the four mitochondrial genome sequences they generated along with mitochondrial genome sequences from 16 woolly mammoth ( Mammuthus primigenius ), two E. maximus ,and 13 Loxodonta individuals, the researchers pieced together a phylogenetic tree. (genomeweb.com)

The mt genome accumulates mutation rates at a faster rate than the nuclear genome and is characterised by fast evolution, maternal inheritance, and a simple structure. (nature.com)
Located within the mitochondrial matrix, and lacking the efficient repair mechanisms available to nuclear DNA, mtDNA has a relatively high rate of mutation. (medscape.com)

Recent molecular analyses of mt genome sequences have provided selectable hypotheses for the phylogenetic relationships of nematodes based on 18S rRNA. (nature.com)
Here, we conducted comparative analyses of nuclear and mitochondrial genomes of seven L. infantum isolates from Tunisia with the aim to gain insight into factors that drive genomic and phenotypic adaptation. (hal.science)

abstract = "We report the first two complete mitochondrial genome sequences of the thylacine [Thylacinus cynocephalus), or so-called Tasmanian tiger, extinct since 1936. (psu.edu)

Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. (wikipedia.org)
Using the conserved cytochrome b (Cytb) and cytochrome c oxidase subunit I (coxI) proteins encoded by the mitochondrial genome, phylogenetic analysis revealed that B. duncani defines a new lineage among apicomplexan parasites distinct from B. microti, Babesia bovis, Theileria spp. (pacb.com)

Although the S. purpurea chloroplast genome has been published (Carlson et al. (springeropen.com)

Here we report the first known completed sequence and annotation of the apicoplast and mitochondrial genomes of B. duncani. (pacb.com)
Annotation of the apicoplast and mitochondrial genomes of B. duncani identified targets for development of effective therapies. (pacb.com)

Predicted to be involved in isoleucyl-tRNA aminoacylation and mitochondrial translation. (mcw.edu)

Through his pioneering research, Svante Pääbo accomplished something seemingly impossible: sequencing the genome of the Neanderthal, an extinct relative of present-day humans. (nobelprize.org)
However, studies of the relationship between present-day humans and the extinct Neanderthals would require the sequencing of genomic DNA recovered from archaic specimens. (nobelprize.org)
This enabled them to identify a Zinc finger protein (ZNF) specifically expressed in humans and not chimpanzees and demonstrate that the human-specific ZNF (named ZNF558) is crucial for both mitochondrial homeostasis and early human brain development. (lu.se)
A particularly intriguing piece of information provided in this study, is that the ZNF558 expression is controlled by a non-coding DNA sequence, which is structurally different between humans and chimpanzee. (lu.se)
In contrast to the coding parts of our genomes, where humans and chimpanzees are close to identical, the non-coding parts show great interspecies variation. (lu.se)
This study provides a concrete example of how the non-coding part of the genome can influence the process of brain development differently between humans and chimpanzees. (lu.se)

The mt genome was sequenced, assembled, and annotated as a circular-mapping DNA molecule. (springeropen.com)

This study also includes a phylogenetic analysis based on 14 concatenated core mitochondrial proteins. (umd.edu)
A complete mitochondrial genome sequence of Ogura-type male-sterile cytoplasm and its comparative analysis with that of normal cytoplasm in radish (Raphanus sativus L. (biomedcentral.com)
Our analysis presents the most definitive mutational landscape of mitochondrial genomes and identifies several hypermutated cases. (lu.se)
1997 ). The comparative analysis of plant mt genomes enhances our understanding of genome rearrangement and DNA transfer mechanisms, and of phylogenetic diversity. (springeropen.com)
We designed primers for nested PCR analysis of the targets based on flanking nucleotide sequences. (cdc.gov)
Mitochondrial DNA (mtDNA) analysis plays a specialized role in forensic applications, overcoming certain limitations of autosomal DNA markers. (avhandlingar.se)
MycoSNP: A Portable Workflow for Performing Whole-Genome Sequencing Analysis of Candida auris. (cdc.gov)
The overarching aim of the Epigenetics common cause of gastric cancer, Pan-cancer genoMe and Group (EGE) is to advance the which is the third most common cause tranScriPtoMe analySiS and understanding of the role of epigenetic of cancer-related deaths worldwide. (who.int)

With the development of next generation sequencing (NGS) technologies, such as the Roche and Illumina platforms, new strategies are being used to characterize plant mitochondrial genomes. (springeropen.com)

The rapid development of DNA sequencing technologies has driven a revolution in our understanding of this highly complex and diverse group of diseases ( Devita and Rosenberg, 2012 ). (frontiersin.org)
Cardiac involvement in mitochondrial diseases. (medscape.com)
This group includes lysosomal storage disorders, various mitochondrial diseases, other neurometabolic disorders, and several other miscellaneous disorders. (medscape.com)

Despite very high sequence similarity with the mtDNA of Acanthamoeba castellanii Neff, in contrast to Acanthamoeba polyphaga Linc Ap-1, the determined DNA sequence revealed a complete absence of introns. (kingston.ac.uk)
[ 2 ] The mitochondrial genome is remarkably concise, containing little noncoding capacity and no introns. (medscape.com)

2001). "Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. (wikipedia.org)

the scientists then used that information to develop mitochondrial sequencing of the other bears and to construct phylogenies showing that the ancient polar bear evolved within the lineage of brown bears. (sciencedaily.com)
The strongly supported mitochondrial and nuclear DNA phylogenies clearly demonstrate that Palaeoloxodon antiquus is more closely related to Loxodonta than to Elephas ," the researchers wrote in their paper. (genomeweb.com)

Continuous admixture of populations from Belgium, Italy, and Hungary was highlighted, additionally, haplotype diversity and clustering indicate a separation of German sequences from other populations, pointing to an independent introduction of Ae. (fmach.it)

Nucleotide sequence alignment led to the identification of 4 sequence types at locus CYC3, 10 at locus CYC13, 2 at locus CYC15, 8 at locus CYC21, and 4 at locus CYC22 ( Technical Appendix Table 2). (cdc.gov)

See how the University of Washington used HiFi sequencing to uncover a key finding about ALS and the human genome. (pacb.com)
The BN rat sequence is the third complete mammalian genome to be deciphered, and three-way comparisons with the human and mouse genomes resolve details of mammalian evolution. (nih.gov)
The relative quantity of mtDNA in a cell, or copy number (mtDNA-CN), is associated with mitochondrial function, human disease, and mortality. (prinsesmaximacentrum.nl)
and estimation of the age of the human race from polymorphism rates, Y-chromosome diversity, linkage block size, and divergence of duplicate sequences. (icr.org)
The completion of the first human genome drafts (Yamey 2000 ) was just a start of the modern DNA sequencing era which resulted in further invention, improved development toward new advanced strategies of high-throughput DNA sequencing, so called the " high-throughput next generation sequencing " (HT-NGS). (springer.com)
Year 2011 is celebrated as the 10th anniversary since the human genome was first sequenced ( www.nature.com/natureconferences/hg10years/index.html ). (springer.com)
In areas where human and cattle schistosomiasis overlap and are transmitted in close proximity, this mitochondrial assay will be a valuable identification tool for epidemiological studies, especially when used in conjunction with other nuclear diagnostic markers. (imperial.ac.uk)
GENOMIC AND MITOCHONDRIAL DNA IN FORENSIC DENTISTRY The genomic DNA is found in the nucleus of each cell in the human body and represents a DNA source for most forensic applications. (bartleby.com)
By the end of the 1990's, almost the entire human genome had been sequenced. (nobelprize.org)
The first cancer exomes were sequenced soon after the completion of the Human Genome Project in 2001. (frontiersin.org)
Sequence and organization of the human mitochondrial genome. (medscape.com)
Johan Jakobsson and his team tries to understand how the human brain is so different in complexity and size to the brains of other great apes, even though our coding genome is almost identical. (lu.se)

Further, we highlight different examples of how these models have shown alterations in neurogenesis, neuronal maturation, neuronal connectivity and synaptic impairment as well as mitochondrial dysfunction and dysregulation of miRNAs in SZ patient-derived cultures compared to controls. (lu.se)

Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)

Use whole genome sequencing to study how and why certain fungi spread and make people sick. (cdc.gov)

How can a heterogeneous group of mitochondrial deletions lead to a similar phenotype? (medscape.com)

insights from mitochondrial genome sequencing. (hal.science)
Studies of plant mt genomes can, therefore, provide unique insights into organelle evolution. (springeropen.com)
Insights into the evolution and drug susceptibility of Babesia duncani from the sequence of its mitochondrial and apicoplast genomes. (pacb.com)
To do this, scientists have developed "single-cell RNA sequencing" (scRNA-seq), which provides unprecedented insights for basic and biomedical research, and even drug development. (epfl.ch)

With the ongoing developments of high throughput sequencing machines and advancement of modern bioinformatics tools at unprecedented pace, the target goal of sequencing individual genomes of living organism at a cost of $1,000 each is seemed to be realistically feasible in the near future. (springer.com)

Prevalence of large-scale mitochondrial DNA deletions in an adult Finnish population. (medscape.com)
Mitochondrial DNA deletions in progressive external ophthalmoplegia and Kearns-Sayre syndrome. (medscape.com)
Kearns Sayre Syndrome (OMIM #530000) occurs as a result of large-scale single deletions (or rearrangements) of mitochondrial DNA (mtDNA), which are usually not inherited but occur spontaneously, probably at the germ-cell level or very early in embryonic development. (medscape.com)
[ 5 ] The deletions vary in size and location on the mitochondrial genome in different individuals, although a common deletion of 4.9kB is present in at least a third of patients with Kearns-Sayre syndrome. (medscape.com)

Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA). (atcc.org)

Genome Insider: A Shrubbier Version of Rubber Hear from the consortium working on understanding the guayule plant's genome, which could lead to an improved natural rubber plant. (doe.gov)

Thirty two haplotypes were formed from 89 D-loop sequences of Proto Malay individuals. (scialert.net)
Most Kanaq and Seletar sequences were merged into tribe specific haplotypes, showing close relationships within tribes. (scialert.net)

Risk of developing a mitochondrial DNA deletion disorder. (medscape.com)

The mitochondrial (mt) genome has drawn increased attention during the genomic and now post-genomic eras owing to its maternal pattern of inheritance and unique evolutionary features, and is often used for the phylogenetic study of plants (Gualberto et al. (springeropen.com)

Phylogenetic relationships among sequence types of Cyclospora cayetanensis at 5 microsatellite loci: A) CYC3, B) CYC13, C) CYC15, D) CYC21, and E) CYC22. (cdc.gov)

Whole-genome sequencing of Acanthamoeba polyphaga Linc Ap-1 resulted in a draft assembly of the chromosomal DNA and a complete sequence of the mitochondrial DNA (mtDNA). (kingston.ac.uk)
The complete sequence of the rice (Oryza sativa L.) mitochondrial genome: frequent DNA sequence acquisition and loss during the evolution of flowering plants. (bioseek.eu)

Tracing the Evolution of Shiitake Mushrooms Understanding Lentinula genomes and their evolution could provide strategies for converting plant waste into sugars for biofuel production. (doe.gov)
Their comparison serves as a model system for understanding the processes of genome evolution. (doe.gov)
Additionally, we compared the S. purpurea mt genome to several previously published genomes to gain enhanced understanding of the evolution of organellar genomes. (springeropen.com)
The genomic information and evolution of the mitochondrial genomes of metazoans is well documented. (biomedcentral.com)
Knowledge on the evolution of mitochondrial DNA (mtDNA) in single-celled organisms on the other hand is more scarce. (biomedcentral.com)
In order to promote the understaning of the mitochondrial genome evolution of single-celled eukaryotes, it is necessary to fill the large existing gaps of knowledge and data. (biomedcentral.com)

Mitochondrial copy number varies greatly within and across cancers and correlates with clinical variables. (lu.se)
Our study lays a foundation for translating mitochondrial biology into clinical applications. (lu.se)

Unique sequences generated from the 5 MLST loci were deposited in GenBank (accession nos. (cdc.gov)

Prevalence of genetically confirmed skeletal muscle channelopathies in the era of next generation sequencing. (ucl.ac.uk)
Next generation sequencing technology now allows us to address these challenges in a new way. (otago.ac.nz)
This article will review recent impact of massively parallel next-generation sequencing (NGS) in our understanding and treatment of cancer. (frontiersin.org)

Anatomy11

Organisms21

Diseases4

Chemicals and Drugs43

Analytical, Diagnostic and Therapeutic Techniques and Equipment41

Phenomena and Processes140

Disciplines and Occupations5

Technology, Industry, Agriculture3

Information Science14

Health Care7

Genes24

Genomics8

Entire mitochondrial genome2

Exome7

Genetic18

Mitochondrion1

Mutations4

Genetics3

Gene9

Genomic sequences1

Nuclear mitochondrial3

Reference genome3

Variants5

Species16

Cytochrome oxidase1

Reveals2

Characterization1

Features of mitochondrial1

Mitochondria5

Woolly mammoth2

Mutation2

Analyses2

Abstract1

Subunit2

Chloroplast genome1

Annotation2

TRNA1

Humans6

Circular-mapping DNA1

Analysis8

Illumina1

Diseases3

Introns2

Proteins1

Phylogenies2

Populations1

Nucleotide sequence1

Human12

Dysfunction1

Comparative1

Fungi1

Heterogeneous group1

Insights4

High-throughput1

Deletions4

Joint Genome In1

Consortium1

Haplotypes2

Deletion1

Evolutionary1

Phylogenetic relationships1

Complete sequence2

Evolution6

Clinical2

Genbank1

Next-generation3