• Backbone head-to-tail cyclization, i.e., setting a covalent peptide bond linking the last amino acid with the first one, is one effective strategy of peptide-based drug design to stabilize the conformation of bioactive peptides while preserving peptide properties in terms of low toxicity, binding affinity, target selectivity, and preventing enzymatic degradation. (bvsalud.org)
  • Purity is determined by HPLC and indicates the presence/absence of contaminating peptides with undesired sequences. (peptide.com)
  • The gross weight of dry peptide doesn't consist of peptide only, but includes non-peptide components such as water, absorbed solvents, counter ions and salts. (peptide.com)
  • Second, and more importantly, we show that it seems possible to elaborate on the information inferred from protein structures to propose effective candidate linker sequences constrained by length and amino acid composition, providing the first framework for the rational design of head-to-tail cyclization linkers. (bvsalud.org)
  • However, very little is known about the sequence-structure relationship requirements of designing linkers for peptide cyclization in a rational manner. (bvsalud.org)
  • Here, we transpose this approach to linkers, allowing head-to-tail peptide cyclization. (bvsalud.org)
  • First we show that given a linker sequence and the conformation of the linear peptide, it is possible to accurately predict the cyclized peptide conformation. (bvsalud.org)
  • Those ions come from the water used for the peptide purification or the buffer used for the mass analysis. (peptide.com)
  • Peak at m/z 209 after MS 3 experiment corresponded to the internal fragment of peptide sequence. (shimadzu-webapp.eu)
  • These different sequences confer varying degrees of microtubule flexibility, the resolution is too lowhigh. (tradingsignalsforex.com)
  • Stereospecific Effects of Oxygen-to-Sulfur Substitution in DNA Phosphate on Ion Pair Dynamics and Protein-DNA Affinity. (utmb.edu)
  • This is because the protein sequence/structure/function is encoded in DNA, and therefore the physicochemical properties of the nanoparticle are also encoded in DNA. (rsc.org)
  • Most attempts made focus on sequence motifs of protein-protein interactions, binding sites, or sequence conservancy. (biomedcentral.com)
  • excellent in a dim download parallel views Based on the Content leaders are the case of international ratio in sequencing MEF ol. (meyer-nideggen.de)
  • SELEX starts by chemical synthesis of a library of random sequences of double-stranded DNA (dsDNA). (encyclopedia.pub)
  • Microbial rhodopsins (Rhs) are transmembrane proteins that utilize light-induced isomerization of their retinal cofactor to function as light-sensitive ion channels, pumps and sensors. (elifesciences.org)
  • The fragmentation characteristics of b7 ions produced from proline-containing heptapeptides have been studied in detail. (bvsalud.org)
  • In the case of very complex proteolytic mixtures, the apparatus might not be able to carry out the fragmentation of all ions, especially those present in a relatively low amount. (medscape.com)
  • [ 19 ] The huge number of fragmentation spectra can be analyzed automatically by devoted software such as Mascot and Sequest, which compare data with protein sequences available in databanks. (medscape.com)
  • 10] L.C.M. Ngoka and M.L. Gross: "Multistep collisionally activated decomposition in an ion trap for the determination of the amino-acid sequence and gas-phase ion chemistry of lithium-coordinated valinomycin", Int. J. Mass Spectrom. (edu.pl)
  • or conjugate, complex, or fusion protein or fusion polypeptide including the same disclosed amino acid sequence derived from bacterium (e.g., mycoplasma, anaplasma, etc. (patentsencyclopedia.com)
  • Amino acid sequence homology provides evidence that the pol sequence encodes reverse transcriptase, integrase and an HIV protease [Tohours, H. et al. (justia.com)
  • 7] L.C.M. Ngoka and M.L. Gross: "Multistep tandem mass spectrometry for sequencing cyclic peptides in an ion-trap mass spectrometer", J. Am. Soc. (edu.pl)
  • Terpenoid synthases are responsible for the cyclization and/or rearrangement of linear isoprenoid diphosphates into cyclic terpenoids [ 12 , 13 ]. (biomedcentral.com)
  • Type I terpene synthases (TPSs) activate these acyclic molecules by the abstraction of diphosphate to produce a reactive allyl cation that can initiate a cascade reaction through typical carbocation chemistry, including cyclisation reactions by intramolecular attack of an olefinic double bond to the cationic centre Wagner-Meerwein rearrangements, and proton or hydride migrations [2] . (beilstein-journals.org)
  • B. PUMMERER CASCADE REACTIONS The combination of a Pummerer-based reaction (04CR2401) followed by an N-acyliminium ion cyclization in tandem to form pyrrolidine-containing ring systems represents a unique method to synthesize heterocycles. (joecolgan.com)
  • the second one bankruptcy is the 12th within the sequence at the natural chemistry of heterocyclic ligands in metal complexes. (joecolgan.com)
  • goals for -aid are a Intermolecular OP inihstuy defined on Formation sequence &, sulphuric chemistry dyes of the Precipitate itself. (windhamny.com)
  • Recent advances in genome sequencing have led to the discovery of numerous terpene synthases, in particular in Basidiomycota like the wood rotting fungus Coniophora puteana , which further enhances the scope for the manufacture of terpenes for industrial purposes. (biomedcentral.com)
  • All class I TPSs share highly conserved sequence motifs in their respective α-domains, such as the aspartate-rich D DXX D (DD) dyad and the ( N , D )D(L,I,V)X( S , T )XXX E (NSE) triad, with both being involved in complexing of three Mg 2+ ions that are essential for catalysis [ 2 ]. (biomedcentral.com)
  • While the amino acid sequences of two TPSs can strongly deviate, their overall structures are very similar and constitute an α-helical fold that was first described for the FPP synthase (FPPS) from chicken ( Gallus gallus ) [10] . (beilstein-journals.org)
  • 8. For a given target molecule, several different reaction sequences may serve to accomplish its synthesis. (msu.edu)
  • Nikbakht A, Amiri K, Khosravi H, Zhou Y, Balalaie S, Breit B . Copper-Catalyzed Cycloisomerization of Unactivated Allene-Tethered -Propargyl Oximes: A Domino Reaction Sequence toward the Synthesis of Hexahydropyrrolo[3,4-]azepin-5(4)-ones. (academictree.org)
  • Such compounds formed host-guest complexes and accelerated phosphoryl transfer reactions when conducted in DMF to promote ion pair formation. (uni-frankfurt.de)
  • Assuming a constant yield for each step, a linear sequence of reactions gives a poorer overall yield than the same number of convergent reactions, as shown in the following diagram. (msu.edu)
  • Many years ago, we investigated anion receptor molecules composed of guanidinium ions. (uni-frankfurt.de)
  • All sesquiterpenoids feature a complex C 15 carbohydrate skeleton which is formed by the cyclization of the universal, aliphatic precursor farnesyl diphosphate (FPP), a reaction catalyzed by enzymes from the sesquiterpene synthase family. (biomedcentral.com)
  • This classical bottom-up strategy is typically based on the preliminary proteolytic digestion of the sample(s) under analysis, followed by high-resolution mono- or multi-dimensional chromatography (or capillary electrophoresis) analysis coupled online with electrospray ionization (ESI) MS. Different analyzers may be coupled: ion trap (IT) triple quadrupole (QQQ) or a Q-TOF. (medscape.com)
  • V. 1,4-Additions A. MICHAEL ADDITION-INITIATED SEQUENCES Dihydropyridine derivatives can be formed from a cascade sequence wherein conjugate addition of enaminoesters occur with a,b-unsaturated carbonyl compounds Sec. (joecolgan.com)
  • An elimination-conjugate addition sequence (gray shaded box) accounts nicely for this result. (msu.edu)
  • Hosseini-Eshbala F, Sedrpoushan A, Breit B , Mohanazadeh F, Veisi H. Ionic-liquid-modified CMK-3 as a support for the immobilization of molybdate ions (MoO): Heterogeneous nanocatalyst for selective oxidation of sulfides and benzylic alcohols. (academictree.org)
  • Nucleotide sequencing of HIV shows the presence of a pol gene in one open reading frame [Ratner, L. et al. (justia.com)
  • The disubstituted olefin 468 reacted in a similar fashion and gave 469 in 70% yield, though the diastereoselectivity (5:1) of the cyclization was significantly diminished. (joecolgan.com)
  • A RISC complex charged with a specific miRNA (symbolized here by red color) would bind to the complementary "red" RNA sequence, not present in the mRNA example shown above. (uni-frankfurt.de)
  • Such conjugates can be used as universal artificial RNA scissors without sequence restrictions for all kinds of analytical purposes. (uni-frankfurt.de)
  • The results have shown that b7 ions undergo head-to-tail cyclization and form a macrocyclic structure. (bvsalud.org)
  • In 2012, Taucher and Breuker (8) were amongst the first to report sequence coverage of full-length transfer RNA (tRNA) using Fourier transform ion cycloctron resonance (FT-ICR), combining data from electron detachment dissociation (EDD) and collision-activated dissociation (CAD) experiments. (biopharminternational.com)
  • Analysis of sequenced microbial genomes show that glsB is primarily confined to the Rhodobacteraceae family, which includes the ecologically important marine Roseobacter clade that are key players in the marine sulphur and nitrogen cycles. (nature.com)
  • Under the collision-induced dissociation (CID) condition, it generates nondirect sequence ions regardless of the position of the proline and the neighboring amino acid residues. (bvsalud.org)
  • MATERIALS AND METHODS: The study was based on network pharmacology, using a combination of RNA-sequencing, "quantity-effect" weighting coefficients, and pharmacodynamic experiments. (bvsalud.org)
  • The goals of this study were to identify expressed sequence tags (EST) in Citrus sinensis (L.) Osbeck corresponding to genes involved in general phenylpropanoid biosynthesis and the key genes involved in the main flavonoids pathways (flavanones, flavones, flavonols, leucoanthocyanidins, anthocyanins and isoflavonoids). (scielo.br)