• The toxin, known as pertussis toxin (or PTx), inhibits G protein coupling that regulates an adenylate cyclase-mediated conversion of ATP to cyclic AMP. (wikipedia.org)
  • However, "B. bronchiseptica" has the genes to express the toxin which shows that "B. bronchiseptica" is closely related to "B. pertussis" [4]. (kenyon.edu)
  • High-Quality Draft Genome Sequences for Five Non-O157 Shiga Toxin-Producing Escherichia coli Strains Generated with PacBio Sequencing and Optical Maps. (cdc.gov)
  • Complete Genome Sequences of Two Shiga Toxin-Producing Escherichia coli Strains from Serotypes O119:H4 and O165:H25. (cdc.gov)
  • Draft Whole-Genome Sequences of Nine Non-O157 Shiga Toxin-Producing Escherichia coli Strains. (cdc.gov)
  • According to the CDC, B. parapertussis causes a pertussis-like illness that is generally milder than pertussis, likely because the bacteria do not produce pertussis toxin. (rapidmicromethods.com)
  • Furthermore, insertion sequence disruption of fhaB was also detected in a previously identified pertussis toxin-deficient isolate that still produced normal levels of Fha. (nabsys.com)
  • Pentacel and VAXELIS contain twice the amount of detoxified pertussis toxin (PT) and four times the amount of filamentous hemagglutinin (FHA) as DAPTACEL. (drugs.com)
  • Pretreatment of cells with pertussis toxin or intracellular infusion of the GDP analogue guanosine-5'-O-(2-thiodiphosphate) was without effects on the control Ca2+ channel current but abolished its hormonal inhibition. (mdc-berlin.de)
  • Four pertussis toxin-sensitive G proteins were identified in membranes of PC-12 cells: two members of the Gi family, Gi1 and Gi2, and two members of the Go family, Go2 and another Go subtype (possibly Go1). (mdc-berlin.de)
  • The present data indicate that activated alpha 2-adrenergic and muscarinic receptors inhibit dihydropyridine-sensitive Ca2+ channels via pertussis toxin-sensitive G proteins without the involvement of a cAMP-dependent intermediate step. (mdc-berlin.de)
  • Maximum-likelihood phylogenetic tree for Bordetella pertussis isolates based on the concatenated multiple sequence alignments of 2,038 core genome multilocus sequence typing loci. (cdc.gov)
  • BP1492 is present in all B. pertussis isolates sequenced to dateand there are not homologues in other Bordetella species. (conicet.gov.ar)
  • Genomic Content of Bordetella pertussis Clinical Isolates Circulating in Areas of Intensive Children Vaccination », PloS One , June 18, 2008. (pasteur.fr)
  • Complete Genome Sequences of 11 Haemophilus ducreyi Isolates from Children with Cutaneous Lesions in Vanuatu and Ghana. (cdc.gov)
  • CDC also performs characterization of B. pertussis isolates by molecular sub-typing, vaccine immunogens deficiency, and whole genome sequencing methods for outbreak support and other public health concerns. (cdc.gov)
  • The United States employs acellular vaccines exclusively, and current Bordetella pertussis isolates are predominantly deficient in at least one immunogen, pertactin (Prn). (nabsys.com)
  • Wholegenome sequencing of deficient isolates revealed putative, underlying genetic changes. (nabsys.com)
  • B. pertussis, B. parapertussis and B. bronchiseptica form a closely related phylogenetical group. (wikipedia.org)
  • B. bronchiseptica" is closely related to "Bordetella pertussis" and "Bordetella parapertussis", causing pertussis or whooping cough in humans [2]. (kenyon.edu)
  • Co-infection of B. pertussis and B. parapertussis can occur but is uncommon. (rapidmicromethods.com)
  • 6] B. parapertussis is not easily distinguished from B. pertussis infection by symptoms, and unlike B. pertussis, usually it is not laboratory confirmed. (rapidmicromethods.com)
  • When paired with Quidel's Virena ecosystem, clinicians will be able to see real-time positive cases at the local level, giving them further insights into disease prevalence for both parapertussis and pertussis. (rapidmicromethods.com)
  • Complete Genome Sequences of Bordetella pertussis Vaccine Reference Strains 134 and 10536. (cdc.gov)
  • Complete Genome Sequences of Four Bordetella pertussis Vaccine Reference Strains from Serum Institute of India. (cdc.gov)
  • Bordetella pertussis is a Gram-negative, aerobic, pathogenic, encapsulated coccobacillus of the genus Bordetella, and the causative agent of pertussis or whooping cough. (wikipedia.org)
  • The paroxysmal cough precedes a crowing inspiratory sound characteristic of pertussis. (wikipedia.org)
  • Bordetella pertussis is a Gram-negative strictly human pathogen of the respiratory tract and the etiological agent of whooping cough. (conicet.gov.ar)
  • Pertussis, or whooping cough, is a very contagious disease caused by the Bordetella pertussis bacteria, which attach to the cilia that line part of the upper respiratory tract, causing inflammation through the release of toxins, which cause airways to swell. (rapidmicromethods.com)
  • 1] Pertussis is spread from person to person through the inhalation of bacteria from an infected person's cough or sneeze. (rapidmicromethods.com)
  • Filamentous hemagglutinin [FHA] is one of the most important immunoprotective antigens of Bordetella pertussis [B. pertussis] and a major component of the acellular pertussis vaccine. (bvsalud.org)
  • DAPTACEL contains the same pertussis antigens, manufactured by the same process, as Pentacel ® [Diphtheria and Tetanus Toxoids and Acellular Pertussis Adsorbed, Inactivated Poliovirus and Haemophilus b Conjugate (Tetanus Toxoid Conjugate) vaccine] and VAXELIS (Diphtheria and Tetanus Toxoids and Acellular Pertussis, Inactivated Poliovirus, Haemophilus b Conjugate and Hepatitis B Vaccine). (drugs.com)
  • High-Quality Draft Genome Sequences for Four Drug-Resistant or Outbreak-Associated Shigella sonnei Strains Generated with PacBio Sequencing and Whole-Genome Maps. (cdc.gov)
  • Complete Genome Sequences of Four Different Bordetella sp. (cdc.gov)
  • Complete Genome Sequences of Two Bordetella hinzii Strains Isolated from Humans. (cdc.gov)
  • Gross, R. (2000) Characterization of a bordetella pertussis diaminopimelate (DAP) biosynthesis locus identifies dapC, a novel gene coding for an N- succinyl-L,L-DAP aminotransferase J Bacteriol 182 3626-31. (unipr.it)
  • Dr. Weigand is a Bioinformatics Health Scientist in CDC's Pertussis and Diphtheria Laboratory, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases. (cdc.gov)
  • His primary research interest is comparative genomics of bacterial pathogens, focusing on Bordetella pertussis . (cdc.gov)
  • An Excel Macro for Determining Allelic and Sequence Types of Bacterial Clones in Multilocus Sequence Typing. (cdc.gov)
  • Scientists have finally observed that some bacterial genetic sequences likely to be involved in bacterial virulence are particularly unstable. (pasteur.fr)
  • CDC is actively researching new laboratory methods to better identify and characterize other Bordetella and Corynebacterium species. (cdc.gov)
  • Advancements in DNA sequencing technologies, particularly next-generation sequencing, have accelerated the discovery of numerous genes from an extensive variety of species. (gatech.edu)
  • Database of sequence clusters obtained as a result of all-against-all BLAST search of proteins in 95 organism species. (biosciencedbc.jp)
  • 2.2 Amino acid sequences of predicted proteins and their annotation for 95 organism species. (biosciencedbc.jp)
  • The number of sequences by organism species contained in the cluster. (biosciencedbc.jp)
  • Protein sequences that are similar to any clustered sequence of 95 organisms species, but not clustered. (biosciencedbc.jp)
  • A tab-delimited text file specifying the ID, length and annotation information of the amino acid sequences of the predicted proteins for 95 organism species. (biosciencedbc.jp)
  • Aspects of the disclosure relate to compositions and methods for amplifying and/or detecting one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens) in a biological sample obtained from a subject. (justia.com)
  • In particular, we have investigated the activation of phage T4 promoters during T4 infection and the regulation of promoters that express virulence gene products in the pathogens Bordetella pertussis and Vibrio cholerae . (nih.gov)
  • TETyper: a bioinformatic pipeline for classifying variation and genetic contexts of transposable elements from short-read whole-genome sequencing data. (cdc.gov)
  • Harmonization of whole-genome sequencing for outbreak surveillance of Enterobacteriaceae and Enterococci. (applied-maths.com)
  • The benefits of whole genome sequencing for foodborne outbreak investigation from the perspective of a National Reference Laboratory in a smaller country. (applied-maths.com)
  • Genome sequencing is a powerful tool for public health, and the COVID-19 Genomic Epidemiology Toolkit provides a practical introduction to genomics for SARS-CoV-2 through a series of short modules specifically tailored to epidemiologists. (cdc.gov)
  • A laboratory-confirmed case meets the clinical case definition and is laboratory-confirmed by isolation of Bordetella pertussis or detection of genomic sequences by means of the polymerase chain reaction (PCR) or positive paired serology. (who.int)
  • Three overlapping coding sequences of FHA antigen were amplified from B. pertussis genomic DNA by PCR. (bvsalud.org)
  • Genomic surveillance and improved molecular typing of Bordetella pertussis using wgMLST. (applied-maths.com)
  • Like B. bronchiseptica, B. pertussis can express a flagellum-like structure, even if it has been historically categorized as a nonmotile bacteria. (wikipedia.org)
  • Bordetella bronchiseptica", stain RB50, is a small (0.4 by 8.0 um), gram negative, rod shaped beta-proteobacteria belonging to the Bordetellae family. (kenyon.edu)
  • Bordetella bronchiseptica" can infect a wide range of hosts causing different diseases and is most commonly found in canines, pigs, and laboratory animals [2]. (kenyon.edu)
  • In vitro peripheral blood mononuclear cells [PBMC] proliferation and IFN- gamma production were assessed in a limited number of healthy adults vaccinated with a commercial acellular pertussis vaccine in response to all purified FHA fragments by H3-Thymidine incorporation and ELISA, respectively. (bvsalud.org)
  • Since 2014, Dr. Weigand has led the laboratory's efforts to characterize novel genetic variation among circulating B. pertussis using high-throughput sequencing and bioinformatics. (cdc.gov)
  • How does an amino acid sequence that is unfolded when it leaves the ribosome manage to fold properly into a highly ordered, lightning-fast enzyme or sturdy structural protein? (anl.gov)
  • For this work, they turned to the N-terminal passenger domain of the pertactin virulence protein from Bordetella pertussis, a typical autotransporter. (anl.gov)
  • BteA, a 69-kDa cytotoxic protein, is a type III secretion system (T3SS) effector in the classical Bordetella, the etiological agents of pertussis and related mammalian respiratory diseases. (nih.gov)
  • The increased number of resulting protein sequences creates an opportunity to expand protein engineering, but also presents a challenge as many gene product molecular functions are poorly annotated. (gatech.edu)
  • A critical component of employing ML for protein engineering is the development of a model that predicts the fitness of a protein given its sequence. (gatech.edu)
  • During Spring of 2023 I found that using a Multilayer Perceptron taking as input a PLM embedding of a mutated amino acid with a protein sequence can accurately predict protein function (Fig. 1). (gatech.edu)
  • Clustering was performed by the method in which the round-robin BLAST search of the above amino acid sequence data is performed, the E-value and the overlap score (the All-against-all BLASTP search of the above amino acid sequence data, and heuristic estimation of a similarity threshold for homologs of each protein by entropy-optimized organism count method ( Bioinformatics 2009 Mar 1;25(5):599-605. ). (biosciencedbc.jp)
  • Pertussis is an infection of the respiratory system characterized by a "whooping" sound when the person breathes in. (wikipedia.org)
  • Rhinovirus, C pneumoniae , and B pertussis are found in the sputum or pharyngeal swab specimens of asthmatic subjects without concurrent symptoms of infection or asthma exacerbation, as well as in some healthy controls. (bmj.com)
  • During infection B. pertussis is exposed to acidic conditions when phagocyte by host cells. (conicet.gov.ar)
  • Other "in-house" PCRs have been developed at individual laboratories to diagnose infections (eg, testing of cerebrospinal fluid [CSF] for herpes simplex virus to diagnose herpes encephalitis and testing of nasopharyngeal wash fluid to diagnose Bordetella pertussis infection), particularly if traditional culture and antigen detection techniques have failed. (medscape.com)
  • B. pertussis is a reemerging pathogen and although there is a vaccine for pertussis, its effectiveness wanes after only a few years. (nih.gov)
  • PacBio's participation in a historic project to sequence 3,000 important strains of bacteria housed at the UK's National Collection of Type Cultures (NCTC) will also be highlighted. (pacb.com)
  • The authors have identified two novel intimin genes ( μ B and ξ R/ β 2B) in typical EPEC strains of serotypes O55 : H51/H− and O119 : H6/H−. The complete nucleotide sequences of the novel μ B and ξ R/ β 2 variant genes were determined. (microbiologyresearch.org)
  • In recent decades, the incidence of B. pertussis infections increased in countries with high vaccine coverage, which could have been caused by 1) genetic changes 2) decreased vaccine uptake or vaccine efficacy 3) waning immunity after immunization 4) better surveillance 5) new sensitive diagnostic tests or 6) age-structured contacts [ 9 , 10 ]. (biomedcentral.com)
  • Despite high vaccine coverage, pertussis cases in the United States have increased over the last decade. (nabsys.com)
  • They made different versions of the pertactin domain by swapping amino acids to change the clustering of hydrophobic amino acids but keep the overall sequence and hydrophobicity ratio the same. (anl.gov)
  • Respiratory specimens were analysed by RT-PCR for rhinovirus, enterovirus and respiratory syncytial virus and by PCR for adenovirus, Chlamydia pneumoniae , Mycoplasma pneumoniae and Bordetella pertussis . (bmj.com)
  • Bordetella pertussis , Chlamydophila pneumoniae , and Mycoplasma pneumoniae . (genomeweb.com)
  • However other regulators systems are present in B. pertussis and needs further research.In the present work we focused on the role of the second messenger c-di-GMP, a key signaling molecule that regulates many importantphysiological processes in bacteria. (conicet.gov.ar)
  • To study the role of this DGC in B.pertussis we interrupted bp1492 gen and evaluate phenotypes previously described as c-di-GMP regulated in other bacteria. (conicet.gov.ar)
  • These efforts will enhance the diagnosis and surveillance of agents causing pertussis or diphtheria. (cdc.gov)
  • Problematic testing for B. pertussis can compromise prevention programs, surveillance activities, vaccine effectiveness studies, and outbreak management. (cdc.gov)
  • These results demonstrate the genetic potential for additional vaccine immunogen deficiency and underscore the importance of continued surveillance of circulating B. pertussis evolution in response to vaccine pressure. (nabsys.com)
  • We are looking forward to updating the community on our recently launched microbial multiplexing workflow, which incorporates two new validated barcoded adapter kits and leverages other advancements with Sequel Binding/Sequencing Kit 2.1 and SMRT Link v5.1.0. (pacb.com)
  • ASM meeting attendees and other interested scientists can apply for free SMRT Sequencing and bioinformatics analysis through the annual Microbial Genomics SMRT Grant Program, sponsored in partnership with the University of Maryland's Institute for Genome Sciences , one of four Certified Service Providers that will be represented at the conference (booth #3224). (pacb.com)
  • Despite thehigh vaccination coverage among children, pertussis is considered a re-emerging disease. (conicet.gov.ar)
  • Data are not available on the safety and effectiveness of using mixed sequences of DAPTACEL and DTaP-containing vaccines from different manufacturers for successive doses of the DTaP vaccination series. (drugs.com)
  • mary vaccination against pertussis proved, with no relapse. (cdc.gov)
  • Recent studies from around the world suggest that B. pertussis infections again follow a seasonal pattern with increased incidence in summer.The aim of this study was to investigate whether respiratory infections caused by B. pertussis in the period from January 2008 to December 2018 also seasonally spread in Germany and if so, when the B. pertussis activity peaked. (biomedcentral.com)
  • The incidence of pertussis has risen steadily over the last few years. (rapidmicromethods.com)
  • 3] Factors that have likely contributed to the increased incidence of pertussis include a decline in vaccine use, waning vaccine-induced immunity in adolescent and adult populations, failure to receive booster shots later in life, and continued circulation of B. pertussis in our population. (rapidmicromethods.com)
  • Schulzke S, Adler H, Bar G, Heininger U, Hammer J. Mycobacterium bohemicum --a the incidence of pertussis. (cdc.gov)
  • The causative agent of pertussis was identified and isolated by Jules Bordet and Octave Gengou in 1906. (wikipedia.org)
  • If there is a pertussis outbreak or suspected diphtheria case that requires assistance, please call the number listed below. (cdc.gov)
  • If there is a pertussis outbreak that requires assistance or you have questions that your local health department cannot answer, contact CDC. (cdc.gov)
  • The disclosure generally relates to methods for detecting the presence of a target nucleic acid sequence. (justia.com)
  • This method has already yielded several algorithms that can successfully predict the effects of a mutation on function given evolutionary information of homologous sequences [2][3]. (gatech.edu)
  • An unrooted tree diagram derived from phylogenetic analysis of the conserved domains of 60 sirtuin sequences from all sirtuin classes. (biomedcentral.com)
  • A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms. (cdc.gov)
  • High-Quality Genome Sequence of an Escherichia coli O157 Strain Carrying an mcr-1 Resistance Gene Isolated from a Patient in the United States. (cdc.gov)
  • We are interested in elucidating the details of gene regulation by the B. pertussis global response regulator, BvgA, which regulates all the known pertussis virulence genes. (nih.gov)
  • Our work has revealed a unique architecture for the interaction of the Bordetella pertussis response regulator (BvgA) with a particular virulence gene promoter. (nih.gov)
  • This hypothesis was borne out by analysis of other autotransporter sequences that also appear to have patterning based on spacing of hydrophobic clusters. (anl.gov)
  • CDC's pertussis and diphtheria laboratorians and epidemiologists can assist health departments during pertussis outbreaks or when a doctor suspects a case of diphtheria. (cdc.gov)
  • CDC actively participates in international capacity building and consultation for both pertussis and diphtheria diagnostics. (cdc.gov)
  • CDC will provide laboratory testing only upon prior communication with the Pertussis and Diphtheria Laboratory indicating the reason for this service. (cdc.gov)
  • Immediate investigation of all pertussis outbreaks with collection of case-based data is also recommended. (who.int)
  • After the introduction of a vaccine against B. pertussis the seasonal pattern with the highest number of infections in the spring to summer months changed. (biomedcentral.com)
  • Our study suggests that clinicians should be more aware of B. pertussis infections in the months from June until September to prevent further transmission to vulnerable family members. (biomedcentral.com)
  • Recently, several studies from around the world suggest that B. pertussis infections again follow a pronounced seasonal pattern. (biomedcentral.com)
  • The aim of this study was to investigate whether respiratory infections caused by B. pertussis have also shown a seasonal distribution in Germany in the period from January 2008 to December 2018 and if so, when the B. pertussis activity peaked. (biomedcentral.com)
  • Humans are the only known reservoir for B. pertussis. (wikipedia.org)
  • Long read sequencing technologies (policy briefings) PHG Foundation, Oct 29, 2018. (cdc.gov)
  • The Solana Bordetella Complete Assay leverages the Helicase-Dependent Amplification (HDA) technology that is resident in Quidel's AmpliVue® molecular product line to generate a fast and accurate test result on the Solana molecular diagnostic instrument. (rapidmicromethods.com)
  • Marienhagen J, Kennerknecht N, Sahm H, Eggeling L. (2005) Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum J Bacteriol 187 7639-46. (unipr.it)
  • Chromosome and Linear Plasmid Sequences of a 2015 Human Isolate of the Tick-Borne Relapsing Fever Spirochete, Borrelia turicatae. (cdc.gov)
  • The complete B. pertussis genome of 4,086,186 base pairs was published in 2003. (wikipedia.org)
  • The final benchmark set contains 12,745 isolated, sequence-resolved insertion (7,281) and deletion (5,464) calls ≥50 base pairs (bp). (nabsys.com)
  • The importance of structural variation in human disease and the difficulty of detecting structural variants larger than 50 base pairs has led to the development of several long-read sequencing technologies and optical mapping platforms. (nabsys.com)
  • Takao T, Hitouji T, Aimoto S, Shimonishi Y, Hara S, Takeda T, Takeda Y, Miwatani T (1983) Amino acid sequence of a heat-stable enterotoxin isolated from enterotoxigentic Escherichia coli strain 18D. (springer.com)
  • Mycobiome diversity: high-throughput sequencing and identification of fungi. (cdc.gov)
  • To help translate these methods to routine research and clinical practice, we developed a sequence-resolved benchmark set for identification of both false-negative and false-positive germline large insertions and deletions. (nabsys.com)
  • Throughout life, factors that interact with RNA polymerase and with sequences close to or within the core promoter itself can alter promoter recognition. (nih.gov)
  • These interactions result in the formation of a remodeled specificity factor for RNA polymerase that recognizes a new promoter sequence. (nih.gov)
  • Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
  • Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
  • B. pertussis has the ability to inhibit the function of the host's immune system. (wikipedia.org)
  • Scientific advances enabled by PacBio long-read sequencing technology will be showcased in more than 25 posters and presentations spanning basic molecular biology and physiology, antimicrobial agents and resistance, environmental science, ecology and evolution, and clinical and public health microbiology. (pacb.com)