Loading...
  • gene
  • Yellow fever structural gene sequences were detected by use of 35 S-labeled negative-sense RNA probe (but not by immunocytochemistry) in 11 of 17 livers from children with fatal illness during the 1965 epidemic in Senegal. (ajtmh.org)
  • Neighbour-joining tree of 16S rRNA gene sequences of species of the genera Comamonas and Delftia with an emphasis on Comamonas testosteroni corresponding to E. coli positions 135-1369. (biomedcentral.com)
  • In situ hybridization is an alternate method in which a "probe," a synthetic nucleic acid with a sequence complementary to the mRNA of the gene, is added to the tissue. (wikipedia.org)
  • However, it is notoriously difficult, and requires knowledge of the sequence of DNA corresponding to the gene of interest. (wikipedia.org)
  • The ISH technique labels specific nucleic acid sequences on a gene that can help detect genetic abnormalities. (wikipedia.org)
  • allele one of multiple alternative forms of a single gene, each of which is a viable DNA sequence occupying a given position, or locus on a chromosome. (wikipedia.org)
  • DNA sequencing dominant double helix duplication Contents: Top 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z electrophoresis endoplasmic reticulum (ER) epigenetics episome epistasis evolution exome exon Any part of a gene that encodes a part of the final mature mRNA produced by that gene after introns have been removed by splicing. (wikipedia.org)
  • PNAs
  • Q-FISH utilizes this unique characteristic of PNAs where at low ionic strengths, PNAs can anneal to complementary single-stranded DNA sequences while single-stranded DNA cannot. (wikipedia.org)
  • mRNA
  • In situ hybridization is a powerful technique for identifying specific mRNA species within individual cells in tissue sections, providing insights into physiological processes and disease pathogenesis. (wikipedia.org)
  • diagnosis
  • Immunocytochemistry is a practical procedure for rapid specific diagnosis of liver stored for months, whereas RNA-RNA hybridization is a sensitive technique which can be applied to material stored for years. (ajtmh.org)
  • Comparative
  • Comparative estimates of disomies X, Y, and 1 were performed in sperm from healthy subjects using FISH, primed in situ labeling (PRINS), and PNA procedures in parallel. (barnardhealth.us)
  • sensitivity
  • Since those original observations, many refinements have increased the versatility and sensitivity of the procedure to the extent that in situ hybridization is now considered an essential tool in cytogenetics. (wikipedia.org)
  • repetitive
  • Apart from studies on crab poly-AT carried out in the early 1960s, Southern was the first to determine the nucleotide sequence of a eukaryotic chromosomal DNA fraction, demonstrating that a guinea pig 'satellite' had an unexpectedly simple repetitive structure based on a sequence of six nucleotides. (wikipedia.org)
  • These and other studies on repetitive DNA sequences enabled him to suggest how non-coding chromosomal DNA may have evolved. (wikipedia.org)
  • specific sequences
  • His 'blot' technique, for the identification of specific sequences among large populations of fragments generated by endonucleases, has found extremely widespread and important applications. (wikipedia.org)
  • coverslip
  • A small volume of the hybridization mixture is placed onto a coverslip and then placed gently onto the microscope slide which contains the fixed cells. (wikipedia.org)
  • amino acid
  • 7. The method of claim 5 wherein the target molecule mimic is a peptide that binds to an 1D5 estrogen receptor-specific monoclonal antibody to produce a detectable reaction and comprising an amino acid sequence QXP, where X can be any amino acid. (google.com)
  • amino acid An organic compound containing amine and carboxyl functional groups, as well as a side chain specific to each individual amino acid. (wikipedia.org)
  • classical genetics cloning codominance codon A series of three nucleotides in a coding region of a nucleid acid sequence which code for a particular amino acid or stop signal during protein synthesis. (wikipedia.org)
  • In general, each codon corresponds to a single amino acid (or stop signal), and the full set of codons is called the genetic code. (wikipedia.org)
  • For example, the codon CAG represents the amino acid glutamine, and TAA is a stop codon. (wikipedia.org)
  • The genetic code is described as degenerate, or redundant, because a single amino acid may be coded for by more than one codon. (wikipedia.org)
  • Introns are regions of DNA that do not code for the amino acid sequence of a protein and were long considered to be 'junk DNA' lacking purpose. (wikipedia.org)
  • technique
  • The most frequently employed technique has been observed to be DNA sequence analysis, which aids in the determination of genotypic changes implicated in genetic diseases. (grandviewresearch.com)
  • As no assumptions are made about the sequence of the origins, this technique is particularly useful for mapping origins in eukaryotes, which are not thought to have precisely defined initiation sequences. (wikipedia.org)
  • 1990
  • These sequences, which ranged from 30 to 150 kilobases in size, were first identified in laboratory mice by David Kipling and Howard Cooke in 1990. (wikipedia.org)
  • fixative
  • The hypotonic solution is then removed by centrifugation and resuspended in a methanol/glacial acetic acid fixative. (wikipedia.org)
  • molecule
  • Carnitine is a molecule that play an essential role in the transport of activated fatty acids across the inner mitochondrial membrane where they are metabolized. (wikipedia.org)
  • vertebrates
  • However, extremely long regions of telomere sequence were not recognized in vertebrates until over a decade later. (wikipedia.org)
  • The currently accepted terminology for these sequences is "mega-telomeres" Mega-telomeres in vertebrates consist of repeats of a six base-pair sequence, TTAGGG, of DNA. (wikipedia.org)
  • Telomerase RNAs differ greatly in sequence and structure between vertebrates, ciliates and yeasts, but they share a 5' pseudoknot structure close to the template sequence. (wikipedia.org)
  • rRNA
  • A short survey of 16S rRNA sequence diversity of C. testosteroni was carried out. (biomedcentral.com)
  • Fig. 1 shows a phylogenetic tree based on 16S rRNA sequences of species of the genera Comamonas and Delftia , with an emphasis on sequences of C. testosteroni isolates with more than 1200 bp length available from Ribosomal Database Project II. (biomedcentral.com)
  • Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al. (wikipedia.org)
  • human
  • This adaptation constituted an interesting challenge because of the particularities of human spermatozoa nuclei in terms of genomic compaction and accessibility of DNA sequences. (barnardhealth.us)
  • While mouse Tyrp1 possesses dihydroxyindole carboxylic acid oxidase activity, the function in human melanocytes is less clear. (wikipedia.org)
  • organisms
  • Parasitology, Vol. 3, 33-46 (1981) and Arnot et a., idem, 47-56 to identify Leishmania organisms by culturing organisms from cutaneous lesions, isolating from the cultures kinetoplast DNA (kDNA) , subjecting it to digestion with a restriction enzyme, then subjecting it to hybridization with labelled kDNA from a known organism. (google.com)
  • Because these label-free technologies do not require knowledge of a particular genetic sequence, they offer specific advantages for characterizing the genetic or protein material of bacterial organisms directly, as well as minimizing the technical complexity of the workflow. (aacc.org)
  • Due to sequence similarity between closely related organisms, higher temperatures are required to melt such DNA hybrids when compared to more distantly related organisms. (wikipedia.org)
  • claim 1 wherein
  • 2. A composition as in claim 1 wherein said second polynucleotide sequence comprises a vector polynucleotide sequence. (google.com)
  • 3. A composition as in claim 1, wherein said first polynucleotide sequence is covalently linked to said second polynucleotide sequence in a chromosome. (google.com)
  • 8. A composition as in claim 1, wherein said third polynucleotide sequence is present in an amount by weight from about 100 to about 1000 fold greater than said second polynucleotide sequence of said composition. (google.com)
  • 9. A composition as in claim 1, wherein said third polynucleotide sequence is from about 50 to about 250 nucleotides in length. (google.com)
  • restriction
  • A telomere array is a unique arrangement of telomeres within a sample (cell, individual, etc.) that is defined by the number of sequence repeats, the pattern of fragments given by restriction digest, the chromosome on which it is found, and the specific location of the sequence on that chromosome. (wikipedia.org)
  • composition
  • This mass is then used to determine the amplicon's nucleic acid base composition from a mathematical algorithm that incorporates the combination of masses of the four nucleic acid bases (A, C, T, and G), along with the reverse complement of the DNA strand, to predict the amplicon's composition. (aacc.org)
  • identification
  • Rapid identification of a microorganism is achieved by hybridization of DNA from a sample containing whole microorganisms with a kabelled specimen of species-specific substantially non-cross-hybridizing DNA from a known microorganism. (google.com)