• It possesses an N-terminal pleckstrin homology (PH) domain that interacts with equal affinity with the second messengers PtdIns(3,4,5)P3 and PtdIns(3,4)P2, generated through insulin and growth factor-mediated activation of phosphoinositide 3-kinase (PI3K). (rcsb.org)
  • Phosphatidylinositol 3-kinase type 2α (PI3KC2α) is an essential member of the structurally unresolved class II PI3K family with crucial functions in lipid signaling, endocytosis, angiogenesis, viral replication, platelet formation and a role in mitosis. (nature.com)
  • Complexes of Vps34, the sole class III PI3K member, produce PI 3-phosphate (PI(3)P) in the endolysosomal system and during autophagy to regulate vesicle-mediated sorting en route to lysosomes 1 . (nature.com)
  • Differentially regulated genes include numerous factors within the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signalling pathway that PTEN counter-regulates. (biomed.news)
  • These guanosine triphosphatases bind GTP causing the activation of mitogen activated protein kinase (MAPK), phosphoinositide-3-kinase (PI3K) and Ras-like (RAL) pathways [ 1 ]. (biomedcentral.com)
  • Homo-oligomers are macromolecular complexes constituted by only one type of protein subunit. (wikipedia.org)
  • Orthologous to human PIK3C2A (phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha). (nih.gov)
  • The IREM-1 (CD300f) inhibitory receptor associates with the p85alpha subunit of phosphoinositide 3-kinase. (nih.gov)
  • This is accompanied by increased, VAMP3-dependent secretion of MT1-MMP, enhanced degradation of extracellular matrix, and increased cell invasion. (nature.com)
  • Storage protein degradation is induced by cold temperature, and the accumulation of a soluble N fraction, especially glutamine, occurs. (globalsciencebooks.info)
  • FTS blocks Ras association with the cellular membrane and renders Ras protein susceptible to proteasomal degradation. (checkpointinhibitor.com)
  • Gain-of-function variants in the three RAS genes have been found in 27 % of human cancers [ 3 ]. (biomedcentral.com)
  • From experimentally validated target genes of these 86 miRNAs, pan-sensitive and pan-resistant genes with concordant mRNA and protein expression associated with in-vitro drug response to 19 NCCN-recommended breast cancer drugs were selected. (cdc.gov)
  • The mRNA and protein expression of the 26 genes was associated with sensitivity or resistance to 18 NCCN-recommended drugs for treating breast cancer. (cdc.gov)
  • The PI 3-kinase PI3KC2α regulates mouse platelet membrane structure and function independently of membrane lipid composition. (nih.gov)
  • The binding of PKB to PtdIns(3,4,5)P3/PtdIns(3,4)P2 recruits PKB from the cytosol to the plasma membrane and is also thought to induce a conformational change that converts PKB into a substrate that can be activated by the phosphoinositide-dependent kinase 1 (PDK1). (rcsb.org)
  • Here, we have studied the function of WDFY2, a cytosolic protein that has been described to reside on endocytic vesicles close to the plasma membrane 6 . (nature.com)
  • The mechanistic basis for the ability of class II PI3Ks to recognize PI(4)P as a substrate to directly produce local pools of PI(3,4)P 2 at defined endocytic membrane nanodomains is unknown. (nature.com)
  • The C2 domain is thought to be involved in calcium-dependent phospholipid binding [ ( PUBMED:8253763 ) ] and in membrane targetting processes such as subcellular localisation. (embl.de)
  • MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
  • New technologies for purifying membrane-bound protein complexes in combination with cryo-electron microscopy (EM) have recently allowed the exploration of such complexes under near-native conditions. (researchgate.net)
  • In particular, polymer-encapsulated nanodiscs enable the study of membrane proteins at high resolution while retaining protein-protein and protein-lip. (researchgate.net)
  • Particular C2s appear to bind phospholipids, inositol polyphosphates, and intracellular proteins. (embl.de)
  • Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)
  • We observed the proteins internalization within macrophages and their capacity to eradicate the intracellular mycobacterial infection at a low micro-molar range. (frontiersin.org)
  • This is characterized by marked changes in certain residues making up the phosphoinositide-binding site, formation of a short a-helix in variable loop 2, and a movement of variable loop 3 away from the lipid-binding site. (rcsb.org)
  • WDFY2 localizes to actin-stabilized endosome tubules positive for the small GTPase RAB4 and shows a preference for highly curved membranes enriched for the lipid phosphatidylinositol 3-phosphate (PtdIns3P). (nature.com)
  • Phosphoinositide 3-kinases (PI3Ks) are a family of lipid-modifying enzymes that phosphorylate the 3′-OH group of inositol phospholipids and play key roles in physiology ranging from cell growth and metabolism to organismal development. (nature.com)
  • Lipid-specific oligomerization of the Marburg virus matrix protein VP40 is regulated by two distinct interfaces for virion assembly. (uci.edu)
  • FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)
  • Adipose tissue signals to brain, liver, and muscles to control whole body metabolism through secreted lipid and protein factors as well as neurotransmission, but the mechanisms involved are incompletely understood. (biomed.news)
  • Phosphatidylinositol 3-kinase inhibitors (PI3Ki) are approved for relapsed chronic lymphocytic leukemia (CLL). (biomed.news)
  • A hypoxic environment devoid of nutrients prevents the cell undergoing energy dependent apoptosis and cells become necrotic. (bmj.com)
  • Apoptosis regulatory proteins are delicately balanced. (bmj.com)
  • The key regulator of this process, hypoxia inducible factor 1 (HIF-1), can initiate apoptosis by inducing high concentrations of proapoptotic proteins, such as BNIP3, and can cause stabilisation of p53. (bmj.com)
  • The Drosophila retinoblastoma protein, Rbf1, induces a debcl and drp1 -dependent mitochondrial apoptosis. (sdbonline.org)
  • Both proteins can localize at the mitochondrion , but the way they control apoptosis still remains unclear. (sdbonline.org)
  • Interestingly, rbf1 -induced apoptosis leads to a debcl - and drp1 -dependent Reactive Oxygen Species production, which in turn activates the Jun Kinase pathway to trigger cell death. (sdbonline.org)
  • These results provide a mechanism by which Drosophila Bcl-2 family proteins can control apoptosis and shed light on a link between Rbf1 and mitochondrial dynamics, in vivo. (sdbonline.org)
  • This group of proteins is responsible for cell proliferation, differentiation and inhibition of apoptosis. (biomedcentral.com)
  • Downstream signalling is controlled by both RAS-intrinsic GTP hydrolysis, guanine nucleotide exchange factors (GEFs) that catalyse hydrolysis and GTPase-activating proteins (GAPs) [ 2 ]. (biomedcentral.com)
  • Clinical follow-up revealed thrombocytopenia, lymphopenia and increased polyclonal immunoglobulins and C-reactive protein. (biomedcentral.com)
  • Myosin filaments act as molecular motors and by binding to actin enables filament sliding. (wikipedia.org)
  • PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress. (nih.gov)
  • Cancer cells secrete matrix metalloproteinases to remodel the extracellular matrix, which enables them to overcome tissue barriers and form metastases. (nature.com)
  • They can be found in telomeric proteins (6), in proteins of the extracellular matrix (7)and seemingly every macromolecular complex in between. (insulin-receptor.info)
  • Over the right time that we have worked to develop the Eukaryotic Linear Motif resource ELM, our conviction is continuing to grow that you will see more than a million LM situations in a ACY-1215 kinase inhibitor higher eukaryotic proteome. (insulin-receptor.info)
  • Experimentalists are similarly afflicted: while trying to identify LMs, they are likely to spend a lot of resources, time and ACY-1215 kinase inhibitor effort carrying out experiments within the false motif candidates, which usually vastly outnumber the genuine ones in any set of proteins of interest (1). (insulin-receptor.info)
  • The microbiome associated with healthy individuals of the red seaweed Laurencia dendroidea can fix nitrogen and provide relevant amino acids and vitamins to the seaweed ( 3 ). (asm.org)
  • A cDNA of -amylase was obtained by RT-PCR, and the deduced amino acid sequence has a high similarity (60% identity) to those of rice and maize. (globalsciencebooks.info)
  • The essence of their function is usually embodied in the linear amino acid sequence and is not dependent on the tertiary structural context. (insulin-receptor.info)
  • Its product is a GTP-binding protein which probably activates phospholipase C that generates the internal promoter diazylglycerol for kinase C, thus signalizing cell proliferation [14-16]. (science-connections.com)
  • In the case of the mitochondrial P450 systems, the specific residues involved in the binding of the electron transfer protein adrenodoxin to its reductase were identified as two basic Arg residues on the surface of the reductase and two acidic Asp residues on the adrenodoxin. (wikipedia.org)
  • More recent work on the phylogeny of the reductase has shown that these residues involved in protein-protein interactions have been conserved throughout the evolution of this enzyme. (wikipedia.org)
  • Protein subunits assembly is guided by the establishment of non-covalent interactions in the quaternary structure of the protein. (wikipedia.org)
  • and phosphatidylinositol-3-phosphate biosynthetic process. (nih.gov)
  • WDFY2 localizes to endosomal tubules by binding to membranes enriched in phosphatidylinositol 3-phosphate (PtdIns3P). (nature.com)
  • An choice activator of MEK 1/2 sig naling is protein kinase Ca. To examine RAF265 927880-90-8 the role of PKCa on NO activation of MEK/ERK/Ets one signaling, cells were treated with EGF or 0. (checkpointinhibitor.com)
  • Many molecular processes within a cell are carried out by molecular machines that are built from numerous protein components organized by their PPIs. (wikipedia.org)
  • Additional proteins participate in various processes. (actanaturae.ru)
  • carried out a cardiac hypertrophy study in mice based on transcriptomics, proteomics and protein turnover data. (biomedcentral.com)
  • The ,src oncogene is possibly, like src, involved in the transmission of proliferation signals which, on this evolutionary level, possibly include the phosphoinositide phosphoinositol turnover [15]. (science-connections.com)
  • 3- 5 Therefore, during severe hypoxia or anoxia, the cell initiates a cascade of events that leads to apoptotic cell death, thereby preventing the accumulation of cells with hypoxia induced mutations. (bmj.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Several enzymes, carrier proteins, scaffolding proteins, and transcriptional regulatory factors carry out their functions as homo-oligomers. (wikipedia.org)
  • These domains were first discovered as the loci of phorbol ester and diacylglycerol binding to conventional protein kinase C isozymes, which contain 2 C1 domains (C1A and C1B) in their N-terminal regulatory regions. (embl.de)
  • Within the RNaseA superfamily there is a group of highly cationic proteins secreted by innate immune cells with anti-infective and immune-regulatory properties. (frontiersin.org)
  • Nevertheless, as a consequence of low affinity binary binding interactions, they usually take action in a concerted and cooperative manner, enabling regulatory decisions to be made on the basis of multiple inputs (8C12). (insulin-receptor.info)
  • However, useful advances are now being made in the bioinformatics tools that address the amazing modularity of eukaryotic regulatory proteins. (insulin-receptor.info)
  • All this information enables the creation of large protein interaction networks - similar to metabolic or genetic/epigenetic networks - that empower the current knowledge on biochemical cascades and molecular etiology of disease, as well as the discovery of putative protein targets of therapeutic interest. (wikipedia.org)
  • Our data provides the first structural insight into the mechanism by which the interaction of PKB with PtdIns(3,4,5)P3/PtdIns(3,4)P2 induces conformational changes that could enable PKB to be activated by PDK1. (rcsb.org)
  • Interaction of protein kinase C zeta with ZIP, a novel protein kinase C-binding protein. (embl.de)
  • This gene encodes a member of the CD300 protein family. (nih.gov)
  • DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
  • Here we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. (uci.edu)
  • It has been previously shown that rbf1 pro-apoptotic activity depends on its ability to decrease the level of anti-apoptotic proteins such as the Bcl-2 family protein Buffy. (sdbonline.org)
  • Buffy often acts opposite to Debcl , the other Drosophila Bcl-2-family protein. (sdbonline.org)
  • The RAS family consists of three proteins all sharing a highly conserved N-terminus region: Harvey RAS (HRAS), Kirsten RAS (KRAS) and neuroblastoma RAS (NRAS). (biomedcentral.com)
  • INTRODUCTION Linear motifs (LMs) are short elements embedded within larger protein Rabbit polyclonal to ZFP161 sequence segments that operate as sites of regulation (1C5). (insulin-receptor.info)
  • In many metabolic reactions, a protein that acts as an electron carrier binds to an enzyme that acts as its reductase. (wikipedia.org)
  • Protein-protein interactions (PPIs) are physical contacts of high specificity established between two or more protein molecules as a result of biochemical events steered by interactions that include electrostatic forces, hydrogen bonding and the hydrophobic effect. (wikipedia.org)
  • These interactions between proteins are dependent on highly specific binding between proteins to ensure efficient electron transfer. (wikipedia.org)
  • Another important distinction to identify protein-protein interactions is the way they have been determined, since there are techniques that measure direct physical interactions between protein pairs, named "binary" methods, while there are other techniques that measure physical interactions among groups of proteins, without pairwise determination of protein partners, named "co-complex" methods. (wikipedia.org)
  • A protein may be carrying another protein (for example, from cytoplasm to nucleus or vice versa in the case of the nuclear pore importins). (wikipedia.org)
  • Remarkably, in contrast to all other PH domains crystallized so far, our data suggest that binding of Ins(1,3,4,5)P4 to the PH domain of PKB, induces a large conformational change. (rcsb.org)
  • Enables 1-phosphatidylinositol-3-kinase activity. (nih.gov)
  • Stem cells are characterized by a lower telomerase activity, which enables only partial compensation for the shortening of telomeres. (actanaturae.ru)
  • High activity of src measured as activity of its product, the pp60c-src kinase, was detected in the nervous cell systems of all groups of animals tested. (science-connections.com)
  • Protein kinase B (PKB/Akt) is a key regulator of cell growth, proliferation and metabolism. (rcsb.org)
  • In addition, cell proliferation and angiogenesis will be stimulated, enabling better oxygenation of the tissue. (bmj.com)
  • Chapter 3 Biochemistry and cell biology of calcium channels and signaling involved in plant growth and environmental responses. (globalsciencebooks.info)
  • Longitudinal single-cell sequencing of TP53-deficient gastric organoids similarly indicates progression towards malignant transcriptional programmes. (stanford.edu)
  • Deep sequencing analyses of perturbed (KO and KD) cell lines uncovered the differential regulation of Pten APA by CFIm59 and CFIm68 and further revealed that their divergent functions have widespread impact for APA in transcriptomes. (biomed.news)
  • Cell viability was monitored by detection of cleaved caspase-3 and the CellTiter-Glo assay. (biomed.news)
  • DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
  • The telomeres consist of repeating sequences and a set of special proteins, which interact with these repeats and spatially organize them in a specific manner, resulting in the formation of the nucleoprotein complex known as telomeric heterochromatin [ 6 , 7 ]. (actanaturae.ru)
  • What does this gene/protein do? (cancerindex.org)
  • The seaweed RNA was sequenced, providing an unprecedentedly high coverage of the transcriptome of Laurencia , and the gene expression levels were compared between control and inoculated samples after 24, 48, and 72 h. (asm.org)
  • To define the localization of WDFY2 in the endocytic pathway, we transiently transfected hTERT-RPE1 cells with green flourescent protein (GFP)-WDFY2 and performed structured illumination microscopy (SIM) together with APPL1 and EEA1 visualized with antibodies (Fig. 1a ). (nature.com)
  • Proteins rarely act alone as their functions tend to be regulated. (wikipedia.org)
  • In accordance with its tumor suppressor role, the Retinoblastoma protein pRb can ensure pro-apoptotic functions. (sdbonline.org)
  • The inhibition of porin under the control of neuronal Ddc-Gal4 result in short lifespan and in an age-dependent loss in locomotor function, phenotypes that are strongly associated with Drosophila models of Parkinson disease . (sdbonline.org)
  • Here, we used Aedes aegypti, which is the primary vector of dengue virus (DENV), to ask if carryover effects enable normal function in adults with no microbiota. (uga.edu)
  • We conclude carryover effects do not enable normal function. (uga.edu)
  • A protein complex assembly can result in the formation of homo-oligomeric or hetero-oligomeric complexes. (wikipedia.org)
  • Predicted to be part of phosphatidylinositol 3-kinase complex. (nih.gov)
  • Here, we reveal the direct regulation of Pten APA by the mammalian cleavage factor I (CFIm) complex, which in turn contributes to PTEN protein dosage. (biomed.news)
  • While the general sequence of SNARE complex formation is well-established, our knowledge on. (researchgate.net)
  • Using the links, experts can explore the motifs, proteins, complex structures and associated literature to evaluate whether candidate motifs might be worth experimental investigation. (insulin-receptor.info)
  • Here we report the discovery of a non-malignant somatic KRAS variant using whole exome sequencing in a minimally symptomatic then-8-year-old girl with unexplained massive splenomegaly. (biomedcentral.com)
  • The protein induction of autophagy was analyzed by RT-qPCR, western blot, immunofluorescence, and electron microscopy. (frontiersin.org)
  • Mitochondrial porin , also known as the voltage-dependent anion channel (VDAC), is a multi-functional channel protein that shuttles metabolites between the mitochondria and the cytosol and implicated in cellular life and death decisions. (sdbonline.org)