Sequences are known euchromatic regions. Medical search. Frequent questions

Abstract: '[Researchers] have determined the nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map. (harvard.edu)
P. 2194 middle column 2nd paragraph: 'Genome assembly relied on the use of several types of data, including clone-based sequence, whole-genome sequence from libraries with three insert sizes [2 kb, 10 kb, and 130 kb], and a Bacterial Artificial Chromosome (BAC)-based sequence-tagged site (STS) content map. (harvard.edu)
The combination of these resources resulted in a set of ordered contigs spanning nearly all of the euchromatic region on each chromosome arm. (harvard.edu)
The prevalence of highly repetitive sequences within the human Y chromosome has prevented its complete assembly to date1 and led to its systematic omission from genomic analyses. (pacb.com)
Analyses of the complete genome sequence will significantly add to our knowledge of chromosomes , including more accurate maps for five chromosome arms, which opens new lines of research. (techexplorist.com)
Scientists generated the complete genome sequence using a cell line with identical copies of each chromosome, unlike most human cells , which carry two slightly different copies. (techexplorist.com)
gene is mapped on the wheat chromosome 5B, but, neither obtaining a physical marker nor microdissec-, tion of this chromosomal region gave practical results, [56]. (centrocardiobogota.com)
Affected individuals are missing at least 352,000 base pairs, also written as 352 kilobases (kb), in the q22.3 region of chromosome 9. (medlineplus.gov)
In addition, the high quality of this genome assembly provides a clearer understanding of key genomic features such as a unique repeat content, a scarcity of pseudogenes, an enrichment of zebrafish-specific genes on chromosome 4 and chromosomal regions that influence sex determination. (inra.fr)
We have determined 99.3% of the euchromatic sequence of the X chromosome. (inra.fr)
About 95% of the Y chromosome is composed of the male-specific region of the Y chromosome (MSY), and the other 5% is two pseudoautosomal regions (PAR1 and PAR2) in two ends of this chromosome (Fig. 1 ). (biomedcentral.com)
The X-transposed region is a result of a recent X-to-Y transposition that has preserved 99% similarity to their X chromosome sequences. (biomedcentral.com)
The problem is solved in most eukaryotes by the addition of repeated sequences to the chromosome ends [ 1 ]. (biomedcentral.com)
Drosophila's chromosome ends are capped by arrays of retrotransposons and the adjacent subtelomeric DNA consists of repetitive elements called telomere-associated sequences (TAS) [ 4 ]. (biomedcentral.com)
Most cells contain two copies of each chromosome, one from each parent and each parent's chromosome contributing a different DNA sequence. (thislifemag.com)
An obvious omission is the Y chromosome, because the complete hydatidiform mole cells used to compile this sequence contained two identical copies of the X chromosome. (thislifemag.com)
However, this work is underway and the researchers anticipate their method can also accurately sequence the Y chromosome, despite it having highly repetitive sequences. (thislifemag.com)
This polymorphism is primarily attributed to the various amount of Y heterochromatin, though earlier studies also suggest variation in the euchromatic portion of the chromosome (Power, 1988). (veteriankey.com)
The probes have been used for molecular hybridizations (fluorescence in situ hybridization, or FISH) in clinical cytogenetics, Y chromosome mapping, sequencing, gene discovery, and comparative Y chromosome studies between the horse and other equids/ Perssodactyls. (veteriankey.com)
Therefore, microdissected Y-chromosome probes are good for FISH but not suitable for cloning and sequencing of the male-specific or pseudoautosomal regions of the chromosome. (veteriankey.com)
As of late 2000, the public human sequence was primarily based on approximately 24,000 accessioned bacterial artificial chromosome (BAC) clones covering 97% of the euchromatic portion of the genome [ 6 ]. (biomedcentral.com)
The high degree of similarity between the mouse and human genomes is demonstrated through analysis of the sequence of mouse chromosome 16 (Mmu 16), which was obtained as part of a whole-genome shotgun assembly of the mouse genome. (inrae.fr)
Two assembly strategies-a whole-genome assembly and a regional chromosome assembly-were used, each combining sequence data from Celera and the publicly funded genome effort. (inrae.fr)
The best average estimate was 3,286 Gb based on the sequence of 22 autosomes, one X chromosome, and one Y chromosome (Morton 1991). (blogspot.com)
Recent advances in sequencing technology have produced sequence data covering the repetitive regions in the gaps and the first complete sequence of a human chromosome (X) was published in 2019 [ First complete sequence of a human chromosome ]. (blogspot.com)
Major gene sequencing and the annotation efforts of HGP were finished by 2006, when the chromosome 1 (largest chromosome) sequence and annotations were published. (splice-bio.com)
Translocation of the nucleolus organizer region to the human X chromosome. (cs-tl.de)
The presence of interstitial telomeric sequences in constitutional chromosome abnormalities. (cs-tl.de)
An interstitial nucleolus organizer region in the long arm of human chromosome 7: cytogenetic characterization and familial segregation. (cs-tl.de)
In order to gain insight into the chromosomal distribution of LTR-retrotransposons in the rice genome, a detailed examination of LTR-retrotransposon sequences on Chromosome 10 was carried out. (biomedcentral.com)
The distribution of elements across chromosome 10 is non-random with the highest density (48 elements per Mb) being present in the pericentric region. (biomedcentral.com)
ZOO-FISH and R-banding reveal extensive conservation of human chromosome regions in euchromatic regions of river buffalo chromosomes. (cnr.it)

Segmental duplications in pericentromeric regions are unique in that around 30% of their sequence can be traced to duplications occurring from other chromosomes. (wikipedia.org)
CGH has been widely applied to detect gains and losses of DNA sequences on specific chromosomes in the study of solid tumours, 1 but has not been extensively used to study unbalanced constitutional karyotypes. (bmj.com)
Finally, our data suggest that the boundary between the recombining pseudoautosomal region 1 and the non-recombining portions of the X and Y chromosomes lies 500 kb away from the currently established1 boundary. (pacb.com)
The availability of fully sequence-resolved Y chromosomes from multiple individuals provides a unique opportunity for identifying new associations of traits with specific Y-chromosomal variants and garnering insights into the evolution and function of complex regions of the human genome. (pacb.com)
results can serve as an important argument in favour of the proposal to develop a special program for sequencing the genome chromosomes and for, To estimate the possibility of plant genome mapping using human genome probes, the probes fluorescent in situ hybridization (FISH) of human 18S-28S rDNA (clon 22F9 from the LA-13NCO1 library) was carried out on chromosomes of the spring barleyHordeum vulgareL. (centrocardiobogota.com)
X-degenerate sequences are single copy and broadly expressed genes which were evolved from ancestral autosomes to generate sex chromosomes. (biomedcentral.com)
A cell from a complete hydatidiform mole has two copies of the father's chromosomes only, and the genetic sequence of each pair of chromosomes is identical. (thislifemag.com)
Thirty percent of the genome is in finished form, including the entire sequence of chromosomes 21 and 22 [ 7 ]. (biomedcentral.com)
Comparison of the structure and protein-coding potential of Mmu 16 with that of the homologous segments of the human genome identifies regions of conserved synteny with human chromosomes (Hsa) 3, 8, 12, 16, 21, and 22. (inrae.fr)
The assemblies effectively cover the euchromatic regions of the human chromosomes. (inrae.fr)
In females, all chromosomes remain euchromatic and functional. (wikipedia.org)
Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (embl.de)

The heterochromatin consists mainly of short, simple sequence elements repeated for many megabases, occasionally interrupted by inserted transposable elements, and tandem arrays of ribosomal RNA genes. (harvard.edu)
Our study, together with previous historical and genetic data, suggests that T. infestans was originally a sylvatic species, with large quantities of DNA and heterochromatin, inhabiting the Andean region of Bolivia. (cdc.gov)
However, the spread of domestic T. infestans throughout the non-Andean regions only involved insects with an important reduction of heterochromatin and DNA amounts. (cdc.gov)
Euchromatic breakpoint of In(2)A4 inversion was localized at 105 bp region (chr2L:21182214-21182318) of the second exon of the Mcm10 gene, the heterochromatic breakpoint is located at the block of dodecasatellite in 2L pericentromeric heterochromatin. (refine.bio)
In order to check the effects of heterochromatin on neighbor euchromatic genes and estimate the distance of inactivation spreading, we performed RNA-seq analysis of genes expression in larvae and adults females of genotypes A12/A12 (control) and In(2)A4/In(2)A4. (refine.bio)
The periphery of the mammalian nucleus is delineated by the nuclear envelope and by subjacent domains of compact and repressed heterochromatin separated by more open and active regions in the vicinity of nuclear pores. (frontiersin.org)
In htz1Delta cells, Sir2 and Sir3 spread into flanking euchromatic regions, producing changes in histone H4 acetylation and H3 4-methylation indicative of ectopic heterochromatin formation. (sdbonline.org)
Htz1 is enriched in these euchromatic regions and acts synergistically with a boundary element to prevent the spread of heterochromatin. (sdbonline.org)
2004). This conserved histone variant acts partly to antagonize the spread of silent heterochromatin into euchromatic regions (Meneghini et al. (irpa2006europe.com)
In Arabidopsis cells, most heterochromatin typically aggregates around the centromeric and peri-centromeric regions, forming conspicuous subnuclear foci referred to as chromocenters which are thought to tether transposable elements (TEs) from distant genomic domains [ 6 ] (Fig. 1a ). (biomedcentral.com)

Large-scale sequencing projects have great potential to provide a wealth of knowledge to scientists and the public. (abclonal.com)
For many, the multi-billion endeavor was considered a "moonshot" for biology, but with its successful completion (99% of the euchromatic genome sequenced with 99.99% accuracy) came the launch of many other large-scale sequencing projects such as the Cancer Genome Atlas (2005) or more recently, the Earth Bio-genome Project (2018). (abclonal.com)

To examine this, we generated a high-quality sequence assembly of the zebrafish genome, made up of an overlapping set of completely sequenced large-insert clones that were ordered and oriented using a high-resolution high-density meiotic map. (inra.fr)
The human genome has already been completely sequenced. (ozva.net)
Is the Genome Now Completely Sequenced? (thislifemag.com)

Ampliconic sequences associated with these inversions show differing mutation rates that are sequence context dependent, and some ampliconic genes exhibit evidence for concerted evolution with the acquisition and purging of lineage-specific pseudogenes. (pacb.com)
The above-mentioned ab, of repetitive sequences in plant genomes (up to 99%), makes difﬁcult the application of this technique for, mapping individual plant genes [40]. (centrocardiobogota.com)
Other signs and symptoms related to 9q22.3 microdeletions probably result from the loss of additional genes in the q22.3 region. (medlineplus.gov)
Detailed automatic and manual annotation provides evidence of more than 26,000 protein-coding genes, the largest gene set of any vertebrate so far sequenced. (inra.fr)
We found 1,098 genes in the sequence, of which 99 encode proteins expressed in testis and in various tumour types. (inra.fr)
Of this number, 168 have been explained by mutations in 113 X-linked genes, which in many cases were characterized with the aid of the DNA sequence. (inra.fr)
There is also a region of exceptionally high gene and G + C content including genes paralogous to those in the major histocompatibility complex. (inra.fr)
We have also detected recently duplicated genes that exhibit different rates of sequence divergence, presumably reflecting natural selection. (inra.fr)
Technological limitations meant the original draft human genome sequence covered just the "euchromatic" portion of the genome-the 92% of our genome where most genes are found, and which is most active in making gene products such as RNA and proteins. (thislifemag.com)
These regions were once thought not to contain any important genetic information but they are now known to contain genes that are involved in fundamentally important processes such as the formation of organs during embryonic development. (thislifemag.com)
Among the 200 million newly sequenced base pairs are an estimated 115 genes predicted to be involved in producing proteins. (thislifemag.com)
However, reduction to a single consensus sequence permits placement of genes and other chromosomal structures in their proper positional context. (biomedcentral.com)
The current coverage is, however, sufficient for the Human Genome Project to enter a new phase, in which the entire sequence can be analyzed to identify genes, regulatory regions and other genomic elements and structures. (biomedcentral.com)
Public attention surrounding completion of the draft human sequence has fostered the impression that we are entering a 'post-genomic' era, and that description of genes and their functions is straightforward. (biomedcentral.com)
Analysis of the genome sequence revealed 26,588 protein-encoding transcripts for which there was strong corroborating evidence and an additional approximately 12,000 computationally derived genes with mouse matches or other weak supporting evidence. (inrae.fr)
Although gene-dense clusters are obvious, almost half the genes are dispersed in low G+C sequence separated by large tracts of apparently noncoding sequence. (inrae.fr)
Approximately 20% of LTR retrotransposon sequences lie within putative genes. (biomedcentral.com)
The premature transition from vegetative to reproductive development in mutants results at least in part from a reduction in the expression of the floral repressor genes ((ARP6 Is usually a Member of the Conserved Eukaryotic ARP6 Class Based on overall amino acid sequence similarity, the protein that we have named ARP6 is usually most closely related to members of the ARP6 class. (irpa2006europe.com)

Our revolutionary sequencing technologies combine the completeness of long reads with the accuracy of short reads to provide the most comprehensive view of genomes, transcriptomes, and epigenomes. (pacb.com)
Different variants of FISH can be used to retrieve information on genomes from (almost) base pair to whole genomic level, as besides only second and third generation sequencing approaches can do. (frontiersin.org)
Once the new technology has matured sufficiently to be used routinely to sequence many different human genomes, from different populations, it will be better positioned to make a more significant impact on our understanding of human history, biology, and health. (thislifemag.com)
So even if we could perfectly sequence two genomes, their total lengths would not be the same. (blogspot.com)
From the Human Genome Project and ENCODE, to projects that involve sequencing of millions of human genomes and were announced by the US Obama Administration and Companies such as Craig Venter's Human Longevity Inc., 23andMe and the Chinese BGI. (splice-bio.com)
One of the largest human sequencing projects that has just started is Genomics England's "100000 Genomes Project" that aims at producing genomics, transcriptomics and epigenomics datasets for 100000 cancer or rare disease patients. (splice-bio.com)
This may seem a lot, especially since we got the first human genome just under a decade ago, but projects that involve the sequencing of millions of human genomes were announced by the US Obama Administration and Companies such as Craig Venter's Human Longevity Inc. , 23andMe and the Chinese BGI . (splice-bio.com)
Apart from human genomes, plants, animals, fungi and microbial communities are also being sequenced en masse . (splice-bio.com)
Our closest companions, the microbes living in our guts and on our skin , were profiled by 16S sequencing by the " Human Microbiome Project" , whereas the " 100K Foodborne Pathogen Genome Project " aims at sequencing the genomes of hundreds of thousands of disease causing bacterial and viral isolates . (splice-bio.com)
As for plants, the first massive projects have already sequenced 1000+ Arabidopsis genomes and 3,000 rice varieties (BGI extended the Project to cover 10000 varieties). (splice-bio.com)
Further, University of Alberta plans to sequence 1000 transcriptomes of different plant species , JGI runs a " 1000 Fungal Genome Project" , there's a " Genome10k " Project to sequence 16000 vertebrates, the " i5k " Project to sequence 5000 insect genomes and the "Fish-T1K " project to sequence 1000 fish transcriptomes. (splice-bio.com)

Much of the newly sequenced material is the "heterochromatic" part of the genome, which is more "tightly packed" than the euchromatic genome and contains many highly repetitive sequences that are very challenging to read accurately. (thislifemag.com)

Multiple studies have found a correlation between the location of segmental duplications and regions of chromosomal instability. (wikipedia.org)
In addition, initial sequence analysis reveals highly ordered chromosomal landscapes associated with paralogous gene clusters and distinct functional compartments. (biomedcentral.com)
The chromo and SET domains are conserved sequence motifs present in chromosomal proteins that function in epigenetic control of gene expression, presumably by modulating higher order chromatin. (embl-heidelberg.de)

The array covers 93.7% of the euchromatic (gene-containing) regions of the human genome and substantially improves resolution over previous arrays. (analytica-world.com)
The ﬁrst section introduces sequence-basedanalyses,includinggeneﬁnd-ing, gene family and phylogenetic analy-ses, and comparative genomics approaches. (centrocardiobogota.com)
An intriguing study surveying the transcriptome of murine brain tissues revealed over 1300 imprinted gene loci (approximately 10-fold more than previously reported) by RNA-sequencing from F1 hybrids resulting from reciprocal crosses. (wikipedia.org)
Although genetics have played a dominant role in cancer research, epigenetics (heritable changes in gene function that do not involve alterations in DNA sequence) has become equally important in this field. (aacrjournals.org)
This nucleoplasmic lamin A environment tends to be euchromatic, suggesting distinct roles of lamin A in the regulation of gene expression in peripheral and more central regions of the nucleus. (frontiersin.org)
Comparative genetic maps within the grass family indicate extensive regions of conserved gene content and orders [ 16 ]. (biomedcentral.com)
For instance, Copia elements are abundant in gene-rich euchromatic regions while Gypsy elements are mainly located in Heterochromatic and pericentromeric regions [45]. (portalbubalu.com.br)

The "Human Genome Project" (HGP) started in 1986 and delivered a first genome draft (90% completion) by 2001 and a "compete" sequence (99% of the euchromatic genome with 99.99% accuracy) by 2006. (splice-bio.com)

Besides, it left critical heterochromatic regions unfinished. (techexplorist.com)
The total assembled length is 3.08 billion bp - about 4% smaller than estimates of genome size based on flow cytometry [ 9 ], presumably due to the exclusion of constitutive heterochromatic regions and centromeres. (biomedcentral.com)
2004). Another Ac-LEHD-AFC recent report Ac-LEHD-AFC indicates that ARP6 binds to telomeres and is required to maintain the silencing of transgenes inserted into heterochromatic regions in the telomere but not transgenes in the centromere (Ueno et al. (irpa2006europe.com)

One paper describes a new whole-genome tiling path microarray, which was constructed from the same DNA used to sequence the human genome in 2001. (analytica-world.com)
The release of the draft human genome sequence in 2001 was a seismic moment in our understanding of the human genome, and paved the way for advances in our understanding of the genomic basis of human biology and disease. (thislifemag.com)
After decades of glacial progress, the Human Genome Project achieved its 2001 breakthrough by pioneering a method called "shotgun sequencing," which involved breaking the genome into very small fragments of about 200 base pairs, cloning them inside bacteria , deciphering their sequences, and then piecing them back together like a giant jigsaw. (thislifemag.com)

Since its initial release in 2000, the human reference genome has covered only the euchromatic fraction of the genome. (techexplorist.com)
Overall design: The fractions of small RNAs (19-29 nt) from ovaries of wild type and 11 transgenic lines of Drosophila melanogaster were sequenced using Illumina HiSeq 2000. (refine.bio)
Even if you have accurate reads of 2000 bp there are many possible ways of assembling these reads into a contiguous region. (blogspot.com)

Comparative genomic hybridisation (CGH) is a dual fluorescence in situ hybridisation protocol capable of detecting global gains and losses of genomic regions by competitive DNA hybridisation. (bmj.com)
Ever since we had the first draft human genome sequence, determine the exact sequence of complex genomic regions. (techexplorist.com)
In a recent study, Bi and colleagues identified genomic regions that are associated with the Arabidopsis NUCLEOPORIN1 (NUP1) protein, an inner subunit of the nuclear pore complex (NPC) [ 5 ] responsible for mRNA export that protrudes out into the lamina-like matrix (Fig. 1 ). (biomedcentral.com)

Scientists have currently identified twenty-five of these regions to be directly associated with some form of genetic disorder. (wikipedia.org)
The information is mainly transmitted in the primary sequence of the genome (genetic information), but there are heritable traits that are transmitted by other mechanisms. (plos.org)
According to scientists, having a complete, gap-free sequence of the roughly 3 billion bases (or "letters") in our DNA is critical for understanding the full spectrum of human genomic variation and understanding the genetic contributions to certain diseases. (techexplorist.com)
It offers a detailed understanding of the genetic contributions to certain diseases and uses genome sequence as a routine part of clinical care in the future. (techexplorist.com)
Several next-generation sequencing technologies have been performed to identify the underlying genetic and epigenetic basis of fibroids. (bvsalud.org)
The newly published genome sequence was created using human cells derived from a very rare type of tissue called a complete hydatidiform mole , which occurs when a fertilized egg loses all the genetic material contributed to it by the mother. (thislifemag.com)
Even though sequencing the (almost) complete genome of a human cell is an extremely impressive landmark, it is just one of several crucial steps towards fully understanding humans' genetic diversity. (thislifemag.com)
Single-cell RNA sequencing (scRNA-seq) has the potential to investigate both genetic and nongenetic sources of tumor heterogeneity in a single assay. (pklab.org)
Linkage and genetic association studies can be immediately followed by investigation of candidate regions. (biomedcentral.com)
High-coverage whole-genome sequencing data of a single ethnicity can provide a useful catalogue of population-specific genetic variations, and provides a critical resource that can be used to more accurately identify pathogenic genetic variants. (nature.com)
As a part of the Korean Personal Genome Project (KPGP), we constructed the KoVariome database using 5.5 terabases of whole genome sequence data from 50 healthy Korean individuals in order to characterize the benign ethnicity-relevant genetic variation present in the Korean population. (nature.com)
It is an epigenetic process that involves DNA methylation and histone methylation without altering the genetic sequence. (wikipedia.org)

Cataloging of segmental duplications was originally difficult due to its inconspicuousness, large size, and high degree of sequence similarity. (wikipedia.org)
Computer based sequence similarity searches of the nucleic acid databases are considered to be a more accurate method to identify retrotransposon families and estimate TE content and distribution in rice genome [ 28 - 31 ]. (biomedcentral.com)

Characterization of histone modifications at subtelomeric regions revealed H3 trimethyl-K9, H3 trimethyl-K27, and H4 trimethyl-K20 enrichment. (biomedcentral.com)
The C-terminal region including the post-SET domain is disordered when not interacting with a histone tail and in the absence of zinc. (embl-heidelberg.de)
The structured post-SET region brings in the C-terminal residues that participate in S-adenosylmethine-binding and histone tail interactions. (embl-heidelberg.de)

Adams MD et al, The genome sequence of Drosophila melanogaster. (harvard.edu)
Based on sequence information from the SET domain, we have isolated human (SUV39H1) and mouse (Suv39h1) homologues of the dominant Drosophila modifier of position-effect-variegation (PEV) Su(var)3-9. (embl-heidelberg.de)

Celera never published an updated sequences but IHPG published a "finished" sequence in October 2004. (blogspot.com)

As a logical step following HGP, the "ENCODE" Project was started in 2003 and aimed to identify all functional elements in the human genome , e.g. transcripts, promoters and long-range regulatory regions. (splice-bio.com)
Recently, an international team of scientists from the United States, China, and Canada collaborated on an ENCODE project to survey RNA-binding proteins on the chromatin regions of the human genome. (abclonal.com)
The work was completed as an ENCODE project, which was a large-scale sequencing project that started in 2003 as the logical step to follow the Human Genome Project. (abclonal.com)

The Telomere to Telomere (T2T) consortium recently published a human genome's first complete, gapless sequence. (techexplorist.com)
According to consortium co-chair Adam Phillippy, Ph.D., whose research group at NHGRI led the finishing effort, sequencing a person's entire genome should get less expensive and more straightforward in the coming years. (techexplorist.com)
The sequence of the human nuclear genome has been completed in draft form by an international public consortium consisting of 16 sequencing centers and associated computational facilities [ 1 ]. (biomedcentral.com)
Recently, the consortium has distributed a working draft assembly of the entire genome that removes redundancies, orients sequence fragments and clearly indicates gaps arising from sequencing and assembly. (biomedcentral.com)
International Human Genome Sequencing Consortium (2004) Finishing the euchromatic sequence of the human genome. (blogspot.com)

Now, with two additional decades of work and technological advances, the complete human genome of around 3 billion bases has finally been sequenced without gaps . (ozva.net)
The newly updated sequence fills in most of the remaining gaps, providing the full 3.055 billion base pairs ("letters") of our DNA code in its entirety. (thislifemag.com)
The IHGP sequence covered about 82% of the genome and it contained about 250,000 gaps and millions of sequencing errors. (blogspot.com)
The amount of actual nucleotide sequence in the latest version of the reference genome ( GRCh38.p13 ) is 3,110,748,599 bp and the estimated total size is 3,272,116,950 bp based on estimating the size of the remaining gaps. (blogspot.com)
As I understand it the 5% that is remaining as unsequenced gaps are difficult to sequence primarily because they're highly repetitive in nature, is that correct? (blogspot.com)

Thousands of researchers have developed better laboratory tools, computational methods, and strategic approaches to decipher complex sequences. (techexplorist.com)
The sequences also show an The conventional and marker-assisted breeding (MAB) are the two approaches used to accomplish plant breeding (Breseghello and Coelho, 2013). (centrocardiobogota.com)
(A) Lamina-associated domains (LADs), at the nuclear envelope, schematized from analyses of cell populations using genome-wide approaches such as DamID- or ChIP-sequencing. (frontiersin.org)

Based on evolutionary origin, euchromatic sequences of MSY are divided into three different classes: X-degenerate, X-transposed, and ampliconic sequences. (biomedcentral.com)

The Web site "www.artichokegenome.unito.it" hosts all the available genomic sequences, together with their structural/functional annotations and project information are presented to users via the open-source tool JBrowse, allowing the analysis of collinearity and the discovery of genomic variants, thus representing a one-stop resource for Cynara cardunculus genomics. (researchgate.net)

But this only included the euchromatic regions, which comprise about 92% of the total genome. (ozva.net)

This complete human genome sequence has already provided new insight into genome biology, and I look forward to the next decade of discoveries about these newly revealed regions. (techexplorist.com)
Analysis of high coverage sequencing data (more than 30x) from 10,000 individuals showed that each newly analyzed genome added roughly 0.7MB of new sequences to the human reference genome and contributed an average of 8,579 new SNVs to the existing human variation data set 13 . (nature.com)
By combining 22 newly sequenced faecal metagenomes of individuals from four countries with previously published data sets, here we identify three robust clusters (referred to as enterotypes hereafter) that are not nation or continent specific. (embl.de)

It's now possible to complete the human genome reference sequence by sequencing at least one individual but I'm not sure that the effort and the expense are worth it. (blogspot.com)

Similarly, formerly independent maps, based on cytogenetic banding patterns, meiotic crossovers and radiation hybrids, may be placed within the single consensus sequence. (biomedcentral.com)

One was developed by PacBio, and allows longer DNA fragments to be sequenced with very high accuracy. (thislifemag.com)

Segmental duplications are shown to be flanked on both sides by large homologous repeats, which exposes the region to recurrent rearrangement by nonallelic homologous recombination, leading to either deletion, duplication, or inversion of the original sequence. (wikipedia.org)
It has been shown that the larger and more homologous duplicated sequences lead to more sporadic segmental aneusomy events. (wikipedia.org)

Both techniques produce much longer sequence reads: 1. (techexplorist.com)
The 14.8-billion bp DNA sequence was generated over 9 months from 27,271,853 high-quality sequence reads (5.11-fold coverage of the genome) from both ends of plasmid clones made from the DNA of five individuals. (inrae.fr)
If a repetitive region is significantly longer than the length of the typical read, it's hard to concatenate those reads into a complete sequence. (blogspot.com)
This has been particularly difficult in centromeric regions but with the development of long sequence reads this problem has been overcome. (blogspot.com)

With ChiP sequencing, the scientists found that RNA-binding proteins were in fact predominantly (~60%) localized on active chromatin regions. (abclonal.com)
In metazoans, a complex meshwork of intermediate filament proteins constitutes the so-called lamina at the inner nuclear membrane to which chromatin regions and associated factors are anchored [ 2 ]. (biomedcentral.com)

Velasco I and Wu, J. Q., Single-cell and single-nuclei RNA sequencing as powerful tools to decipher cellular heterogeneity and dysregulation in neurodegenerative diseases. (uth.edu)

Highly repetitive regions are unstable in plasmid/cosmids/BACs so their length and sequences couldn't be accurately determined. (blogspot.com)

The number of segmental duplications in pericentromeric regions is highly variable, having as few as zero. (wikipedia.org)
Twenty-nine of the forty-three pericentromeric regions have some form of segmental duplication totaling 47.6Mb, which is almost a third of all segmental duplications found in the human genome. (wikipedia.org)

Radial placement of beads is determined from, here, lamin A ChIP-sequencing data in fibroblasts. (frontiersin.org)
Both projects make extensive use of next generation sequencing techniques such as RNA-Seq , ChIP-Seq and Bisulfite-Seq . (splice-bio.com)
Precisely at those repetitive regions, a signal about the microarray was apparent, arguing for interference of repetitive sequences with all the probe amplication and hy bridization demanded during the ChIP on chip process, and hence presumably representing a sequence connected artifact. (sodiumchannel-signal.com)

Here, we perform single-cell RNA sequencing (scRNA-seq) on 19 human NB samples coupled with multiplex immunohistochemistry, survival analysis, and comparison with normal fetal adrenal gland data. (pklab.org)
The assembly provides simplified descriptions of the genome, as disparate data sources such as GenBank and numerous expressed sequence tag (EST) and protein databases are unified. (biomedcentral.com)
The public data were shredded into 550-bp segments to create a 2.9-fold coverage of those genome regions that had been sequenced, without including biases inherent in the cloning and assembly procedure used by the publicly funded group. (inrae.fr)
DNA sequence comparisons between the consensus sequence and publicly funded genome data provided locations of 2.1 million single-nucleotide polymorphisms (SNPs). (inrae.fr)
Lines represent the HT events identified from genome short read sequencing data. (portalbubalu.com.br)
Durocher Y, Perret S, Kamen A( 2002) High-level and aortic AbstractRecombinant pLT43 region by multidimensional method of modeling supernatant longitudinal data. (naturheilpraxis-gisbert-fussek.de)

The sequence of these clones is approximately 93% complete to at least 4-fold coverage [ 7 ]. (biomedcentral.com)

The human genome contains around 130 regions, totaling 274Mb and ten percent of the total genome, that are flanked by these intrachromosomal duplications. (wikipedia.org)

Any large-scale sequencing project, even the Human Genome Project, is a major investment requiring global collaboration. (abclonal.com)

According to classic definitions, epigenetics refers to heritable differences between cells or organisms that occur without changes in DNA sequence, and do not depend on different external conditions [1] - [4] . (plos.org)

A 2.91-billion base pair (bp) consensus sequence of the euchromatic portion of the human genome was generated by the whole-genome shotgun sequencing method. (inrae.fr)

Their telomeres are centrally localized, forming nucleolus-associated domains (NADs), whereas their heterochromatic repetitive elements are condensed around centromeric and peri-centromeric regions, forming peripheral chromocenters. (biomedcentral.com)

Using both techniques, scientists could generate the complete human genome sequence. (techexplorist.com)

Regions of the genome which are flanked by repeats represent potential hotspots of genomic instability that are prone to copy-number variation. (wikipedia.org)
Research has also found an increase of segmental duplications within regions of the CNPs showing that the duplications are responsible for the variation in copy number. (wikipedia.org)
The largest heterochromatic region in the human genome, Yq12, is composed of alternating repeat arrays that show extensive variation in the number, size and distribution, but retain a 1:1 copy-number ratio. (pacb.com)
This new human genome sequence is especially useful for studies that aim to establish comprehensive views of human genomic variation or how people's DNA differs. (techexplorist.com)

Anatomy5

Organisms1

Chemicals and Drugs5

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes11

Information Science4

Chromosome32

Chromosomes12

Heterochromatin10

Large-scale Sequencing Projects2

Completely sequenced3

Genes18

Genomes11

Highly repetitive sequences1

Chromosomal3

Gene7

99.991

Heterochromatic regions3

20013

20003

Genomic regions3

Genetic12

Similarity2

Histone3

Drosophila2

Celera1

Encode3

Consortium5

Gaps5

Approaches3

Ampliconic1

Genomics1

Comprise1

Newly3

Reference sequence1

Hybrids1

Fragments1

Homologous2

Reads4

Proteins2

Decipher1

BACs1

Pericentromeric regions2

ChIP3

Data6

Clones1

Total genome1

Human Genome1

Organisms1

Portion1

Repetitive elements1

Scientists1

Variation4