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Miniaturized affinity chromatography: A powerful technique for the isolation of high affinity GAGs sequences prior to their identification by MALDI-TOF MS. (bvsalud.org)
Finally, this original miniaturized analytical workflow coupling miniaturized AT III- affinity chromatography to MALDI -TOF MS detection is able to select, enrich and detect and identify high affinity sequences (mainly DP4 in size length with a high degree of sulfation) from low molecular weight heparin samples. (bvsalud.org)
A more specific selection of GAG sequences can be achieved by increasing the ionic strength during the washing step of affinity chromatography . (bvsalud.org)
Anti-Human RANTES (CCL5)-specific antibody was purified by affinity chromatography and then biotinylated. (fishersci.com)
After expression, the protein is captured by means of immobilized metal affinity chromatography (IMAC). (genengnews.com)
A serine protease was purified from poultry organic dust by benzamidine-agarose affinity chromatography. (cdc.gov)
Analysis of folates using combined affinity and ion-pair chromatography. (cdc.gov)
The corresponding enzyme was purified from grain extracts by affinity chromatography on a pepstatin-Sepharose column. (lu.se)

The figure below demonstrates that the humanized antibody maintains a similar level of affinity when compared to the parental antibody. (news-medical.net)
Affinity of antibody before and after humanization. (news-medical.net)
Nanobody sequences generated from RenNano mice have the highest possible diversity and do not require antibody humanization, which saves time and cost and reduces the risk of failure during later stages of drug development. (businesswire.com)
We show here that the reactivity of a high affinity, virus-neutralizing human antibody against the AD-2 epitope of cytomegalovirus gB can be modified by introducing other Vkappa sequences together with the original VH sequence. (lu.se)
It was also evident that the VH/Vkappa pairing was not promiscuous, since antibody fragments selected by phage display retained light chain sequences very similar to the original hybridoma-derived light chain, proving that a high affinity interaction was very dependent on a co-operativity between both variable domains. (lu.se)

The peptides were then synthesized and evaluated for metal affinity and specificity. (rsc.org)
The affinity of a TCR for a given peptide epitope and the specificity of the binding are governed by the heterodimeric αβ T-cell receptors ( 2 ). (frontiersin.org)
In both TCRα and TCRβ chains, CDR3 loops have the highest sequence diversity and are the principal determinants of receptor binding specificity. (frontiersin.org)
A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)

The mecA gene produces transpeptidase PBP2a (penicillin-binding peptide) that decreases the bacterial affinity for beta-lactam antibiotics. (medscape.com)
The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)

The uniqueness and low cross-reactivity of the Prestige Antibodies ® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. (sigmaaldrich.com)
Translating Ribosome Affinity Purification (TRAP) is a technology to isolate the population of mRNAs associated with at least one 80S ribosome, referred as the translatome. (rna-seqblog.com)
TRAP is based on the expression of an epitope-tagged version of a ribosomal protein and the affinity purification of ribosomes and associated mRNAs using antibodies conjugated to agarose beads. (rna-seqblog.com)
Further purification on a Mono S column followed by activity measurements and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the affinity-purified enzyme preparation contained two active heterodimeric aspartic proteinases: a larger 48k Da enzyme, consisting of 32-kDa and 16-kDa subunits and a. (lu.se)
Further purification on a Mono S column followed by activity measurements and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the affinity-purified enzyme preparation contained two active heterodimeric aspartic proteinases: a larger 48k Da enzyme, consisting of 32-kDa and 16-kDa subunits and a smaller one of 40 kDa, consisting of 29-kDa and 11-kDa subunits. (lu.se)

repeat analysis and multiple antigen sequence typing Coverage among infants in Denmark for the third dose to characterize isolates of Bordetella pertussis strains of pertussis vaccine has traditionally been high (87%- circulating in Denmark during periods with and without 91%) ( 3 ). (cdc.gov)

These findings show that phage display technology might modify the binding properties of pre-existing, high affinity antibodies. (lu.se)

Following humanization, the humanized nanobody maintains a similar level of affinity when compared to the parental nanobody. (news-medical.net)

Using second generation sequencing, instead of measuring relative hybridization of sequences to probes in the case of microarrays or sequencing short segments in the case of SAGE, a researcher can simply sequence the bulk RNA within a sample and measure relative abundances of specific types of RNA by comparing the number of times each RNA molecule was sequenced in a given sample. (wikipedia.org)
Click molecule labels to explore molecular sequence information. (nih.gov)

In this context, this work investigates the use of a new miniaturized monolithic affinity column (poly(GMA-co-MBA) grafted with antithrombin III (AT III)) to specifically capture and elute high affinity sequences contained in low molecular weight heparin ( enoxaparin ) for further on-line characterization. (bvsalud.org)
Recently, high-throughput DNA sequencing has enabled large-scale characterization of TCR sequences, producing detailed T cell repertoire (Rep-Seq) ( 11 ). (frontiersin.org)
They are isolated via SELEX (Systematic Evolution of Ligands by Exponential Enrichment), an evolutionary process that involves iterative rounds of selection and amplification before sequencing and aptamer characterization. (genomicglossaries.com)

Mass spectrometry and amino-terminal sequence analysis identified the purified protease as chicken trypsin II P29. (cdc.gov)

The amino-terminal sequence obtained for P450UK was identical to the sequence deduced from a cDNA encoding CYP2C19, thus identifying P450UK as 2C19. (nih.gov)

Affinity tagging, a popular method for capturing or immobilizing proteins, has several incarnations, none more popular than polyhistidine tagging. (genengnews.com)
NTA and IDA fix transition metal ions to the matrix, where they complex polyhistidine sequences on proteins of interest. (genengnews.com)
RNA binding proteins recognize these sequences and assemble with their mRNA target into mRNPs. (europa.eu)
It is thought that motor or adaptor proteins recognise different features on the surface of mRNPs with different affinities. (europa.eu)

The biosimilar is going to have the same protein sequence . (medscape.com)
The proposed method is novel because it works independently of protein sequence databases and without any prior knowledge about modifica- tions. (lu.se)
PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)
Native or wild-type quaternary protein structure is usually born from a single translated protein sequence with one ordered conformation with downstream protein interactions. (medscape.com)
The mutations change single protein building blocks (amino acids) in the filamin B protein or delete a small section of the protein sequence, resulting in an abnormal protein. (medlineplus.gov)

Folic acid and 5MeTHF concentrations were determined using the affinity/HPLC method with electrochemical (coulometric) detection method Which was developed by us at the USDA Human Nutrition Research Center at Tufts University (Bagley et al. (cdc.gov)

However, specific disruption of each RDRC imposes distinct loss-of-function consequences at the cellular level and has a differential impact on the accumulation of specific 23-24-nt sRNA sequences in vivo. (nih.gov)

An in vitro binding kinetic analysis revealed that these phosphorylation-dependent events were attributable to a decrease in the affinity of phosphorylated Homer3 for its ligand. (jneurosci.org)

HCAbs generated from the RenNano mouse have highly diverse CDR3 sequences and can recognize many epitopes with nM-level high affinities. (businesswire.com)

Whole genome sequencing for predicting Mycobacterium abscessus drug susceptibility bioRxiv, Jan 24, 2018. (cdc.gov)

Nanoscaffolding and nanoquenching properties of thiol-capped gold nanocrystals (GNCs), covalently linked to fluorophore-lebeled oligonucleotide through metal-sulfur bond, were extensively studied for decades to detect specific sequences and single-nucleotide mismatches [ 1 - 3 , 21 , 22 ]. (hindawi.com)

In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)

It's going to bind to that target with the same binding affinity to within a narrow range, possibly an even narrower range than for one of the batches of the innovator. (medscape.com)

Second-Generation DNA sequencing has enabled cost effective, high throughput and unbiased analysis of the transcriptome. (wikipedia.org)
This miniaturized, high binding capacity affinity column allows the specific capture of high-affinity oligosaccharide chains from Enoxaparin , even at low concentrations and with a minimal consumption of AT III. (bvsalud.org)
In this study, we systematically analyzed CD19 CAR-T cells of 10 children with R/R B-ALL enrolled in the CARPALL trial via high-throughput single-cell gene expression and T cell receptor sequencing of infusion products and serial blood and bone marrow samples up to 5 years after infusion. (nature.com)
miRCURY LNA miRNA Power Target Site Blockers are efficient at very low concentrations, and due to their high affinity from LNA enhancement , the target site blockers outcompete miRNAs for their target sites. (qiagen.com)
Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)

I found some XML scripts in rosetta_demos/rosetta_scripts/experimental/computational_affinity_maturation_strategy*, but I don't know if these are what we want. (rosettacommons.org)

It utilizes trends, patterns, sequences, and affinities found in data to make determinations about when and where crimes will occur. (commondreams.org)
[ 3 ] It is also important to understand that the same polypeptide sequence can produce many different patterns of interresidue or intraresidue interactions. (medscape.com)

Higher sequence diversity in the vaginal tract than in blood at early HIV-1 infection. (cdc.gov)

Utility of a portable desiccant system for preservation of fecal samples for downstream 16S rRNA amplicon sequencing. (cdc.gov)

This allows one affinity column to be loaded with the sample and subsequently washed with a pH 7.0 phosphate buffer, while the second is eluted into the analytical column with an acetonitrile gradient at acid pH and into the electrochemical detector. (cdc.gov)

After optimization of the sample preparation and ionization parameters , direct on-line analysis was performed by applying the elution volume released from the miniaturized affinity column (≤1 µL) directly to the MALDI plate. (bvsalud.org)
Affinity kinetic analysis of Parental VHH (Biacore). (news-medical.net)
Separation and partial amino acid sequence analysis of each subunit indicate that the 40-kDa enzyme is formed by proteolytic processing of the 48k Da form. (lu.se)

It was found that many of oligosaccharide chains in enoxaparin were eliminated and that certain chain sequences were retained and enriched. (bvsalud.org)

Time-resolved RNA sequencing methods are applications of RNA-seq that allow for observations of RNA abundances over time in a biological sample or samples. (wikipedia.org)
The assay was a viable alternative approach of qPCR for detecting, quantifying and counting copy number of shorter size DNA sequences to address a wide ranging biological problem in food industry, diagnostic laboratories and forensic medicine. (hindawi.com)
No studies so far practically explored the sequence and mismatch detecting power of the fluorophore-labeled-oligo-nanoparticle conjugates in heterogeneous biological samples. (hindawi.com)

Amino-acid sequence alignment and inhibition studies showed that the barley aspartic proteinase resembles mammalian lysosomal cathepsin D (EC 3.4.23.5). (lu.se)

With Target CPM, Google Ads will optimize bids to show your whole sequence to your audience, which can help to get a higher sequence completion rate. (semrush.com)
Non-target DNA binding affinity scales with PAM density, and sequences fully complementary to the guide RNA but lacking a nearby PAM are ignored by Cas9-RNA. (nature.com)
Competition assays provide evidence that DNA strand separation and RNA-DNA heteroduplex formation initiate at the PAM and proceed directionally towards the distal end of the target sequence. (nature.com)
The biosensor probe identified a target DNA sequence that was several-folds shorter than a typical PCR-template. (hindawi.com)

mRNA localisation requires primary sequences and secondary structures often localised in their 3'UTR (3). (europa.eu)

YouTube video ads sequencing lets you tell your brand story over a series of ads in a specific order, or you can showcase your product message across multiple videos. (semrush.com)
The pH optimum of the affinity-purified enzyme was between 3.5 and 3.9 as measured by hemoglobin hydrolysis and the enzymatic activity was completely inhibited by pepstatin a specific inhibitor of aspartic proteinases (EC 3.4.23). (lu.se)

So I am curious if there is any progress in this area, is there already some stable/pilot protocols of in silico affinity maturation that can be followed to be applied in similar works? (rosettacommons.org)

In analyzing the stability of DNA replication origins in S. cerevisiae (see Stable vs. unstable ARSs in mcm1-1 mutant below) we faced the question of whether one set of sequences has more and/or better binding sites of a particular transcription factor than the other. (biomedcentral.com)

Current sequencing methods allow for detailed samples of T cell receptors (TCR) repertoires. (frontiersin.org)
Determination of the transmembrane topology of a protein starts with a model of the protein based on sequence information and theoretical methods. (lu.se)

Quantitative assessment of the translatome is achieved by direct RNA sequencing (RNA-SEQ), which provides accurate quantitation of ribosome-associated mRNAs and reveals alternatively spliced isoforms. (rna-seqblog.com)

Such an approach requires extensive repertoire sequencing for every condition tested. (frontiersin.org)

Anatomy16

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