Sequence tagged site sequence tagged sites. Medical search. Frequent questions

As we recently reported, analysis of 77 FHWT by whole genome sequencing (WGS, 58 patients) or whole exomic sequencing (WES,19 patients) identified 825 high-quality somatic, non-synonymous variants, with an average of 11 candidate mutations/case (range 2-42) (ref. 6 ). (nature.com)
In total, 2957 new AhMITE1 markers were developed in addition to identifying 465 already reported markers from the whole genome re-sequencing data (WGRS) of 33 diverse genotypes of peanut. (biomedcentral.com)
But, analysis of whole genome re-sequencing (WGRS) data from a large number of diverse genotypes is expected to capture all AIPs when the short reads are analyzed using the computational method polymorphic TEs and their movement detection (PTEMD) [ 23 ] for the de novo discovery of AIPs. (biomedcentral.com)
Targeted sequencing of the S gene, using either NGS or Sanger sequencing, or whole genome sequencing using NGS will provide positive identification of Omicron. (who.int)

A sequence-tagged site (or STS) is a short (200 to 500 base pair) DNA sequence that has a single occurrence in the genome and whose location and base sequence are known. (wikipedia.org)
The completion of the first human genome drafts (Yamey 2000 ) was just a start of the modern DNA sequencing era which resulted in further invention, improved development toward new advanced strategies of high-throughput DNA sequencing, so called the " high-throughput next generation sequencing " (HT-NGS). (springer.com)
For the future direction, we need the in-depth genome sequence information and analysis for most of the mammals, including human to fully understand genome variation of economic traits, genetic susceptibility to diseases, and pharmacogenomics of drug response. (springer.com)
Year 2011 is celebrated as the 10th anniversary since the human genome was first sequenced ( www.nature.com/natureconferences/hg10years/index.html ). (springer.com)
During this period, tremendous success has been achieved in the fields of decoding of human genome, technological advancement of new era of human genome applications, toward personalized genomes and discovery of rare variants, leveraging genome sequencing to impact on cancer researches and mammalian evolution and population structure. (springer.com)
The frequencies of CNVs in these populations were analyzed by oligonucleotide array comparative genome hybridization, quantitative PCR, PCR, DNA sequencing across breakpoints, and single-worm PCR. (biomedcentral.com)

The prediction of transcription factor binding sites in genomic sequences is in principle very useful to identify upstream regulatory factors. (lu.se)

DNA, randomly amplified polymorphic DNA (RAPD) locus sequence types that are not found in human GBS analysis, and multilocus sequence typing (MLST), have strains. (cdc.gov)
This study aimed to develop and validate new AhMITE1 and cleaved amplified polymorphic sequences (CAPS) markers. (biomedcentral.com)
Use of diverse genotypes including the genetically unstable peanut mutants which show hyperactivity of AhMITE1 for marker discovery might detect a large number of AhMITE1 insertion polymorphic sites (AIPs), which could be employed to develop new markers. (biomedcentral.com)

The high-throughput - next generation sequencing (HT-NGS) technologies are currently the hottest topic in the field of human and animals genomics researches, which can produce over 100 times more data compared to the most sophisticated capillary sequencers based on the Sanger method. (springer.com)
Each mutation was verified by Sanger sequencing of PCR products spanning the variants ( Fig. 1 ). (nature.com)

Different proteins were known to have unique sequences, whereas it was thought that all DNA molecules have the same sequence. (proprofs.com)
In contrast to the signal sequences of exported proteins, the bacterial SRP signal sequences are more hydrophobic and are mostly "uncleaved signal sequences" present in membrane proteins that remain in the final protein-chain as transmembrane anchor sequences. (nature.com)
The increase of the hydrophobicity in the signal sequences of E. coli presecretory proteins makes it possible to re-route the SecA dependent preproteins into the SRP targeting cycle 17 . (nature.com)
A method for determining the sequence specificity of DNA-binding proteins. (lookformedical.com)
A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. (lookformedical.com)
Specifically, cullin-7 helps assemble a complex called an E3 ubiquitin ligase, which tags the unneeded proteins for degradation. (medlineplus.gov)
Mutations in the CUL7 or OBSL1 gene prevent the cullin-7 protein from bringing together the components of the E3 ubiquitin ligase complex, interfering with the process of tagging unneeded proteins for degradation. (medlineplus.gov)

It is also called nucleotide sequence. (lookformedical.com)

They are used in shotgun sequencing, specifically to aid sequence assembly. (wikipedia.org)

All DNA sequences for the three genes known to be involved in CMT disease, for healthy individuals and patients, were confirmed by direct sequencing. (hindawi.com)
Nucleic acid sequences involved in regulating the expression of genes. (lookformedical.com)
Cis-acting DNA sequences which can increase transcription of genes. (lookformedical.com)
We developed TS-REX, a database/software system that supports the analysis of tissue and cell type-specific transcription factor-gene networks based on expressed sequence tag abundance of transcription factor-encoding genes in UniGene EST libraries. (lu.se)
The use of expression levels of transcription factor-encoding genes according to hierarchical anatomical classifications covering different tissues and cell types makes it possible to filter out irrelevant binding site predictions and to identify candidates of potential functional importance for further experimental testing. (lu.se)

An examination of the content and distribution of repetitive DNA sequences to identify the location of such sequences within specific chromosomes and their conservation across species of the Pinaceae family. (le.ac.uk)
Fluorescent in situ hybridisation (FISH) on metaphase chromosomes and on extended chromatin will be used to physically locate repetitive as well as single copy DNA sequences on conifer chromosomes and to integrate physical and genetic maps. (le.ac.uk)
From the SNPs on the A03, B01, B02 and B03 chromosomes, 11,730 snip-SNPs (potential CAPS sites) were identified, and 500 CAPS markers were developed from chromosome A03. (biomedcentral.com)

The Arc gene, which contains remnants of a structural GAG retrotransposon sequence, produces a protein that self-assembles into capsid-like structures harboring Arc mRNA. (lu.se)
To enable the tracking of Arc molecules from individual neurons in vivo, we devised an adeno-associated virus (AAV) mediated approach to tag the N-terminal of the mouse Arc protein with a fluorescent reporter using CRISPR/Cas9 homologous. (lu.se)
To enable the tracking of Arc molecules from individual neurons in vivo, we devised an adeno-associated virus (AAV) mediated approach to tag the N-terminal of the mouse Arc protein with a fluorescent reporter using CRISPR/Cas9 homologous independent targeted integration (HITI). (lu.se)

7- 9 All reported mutations are single nucleotide alterations leading to splice site, missense, nonsense, or frameshift mutations, or intra-exonic deletions and insertions of up to four nucleotides, which have been detected through exonic PCR amplifications and sequencing. (bmj.com)

When STS loci contain genetic polymorphisms (e.g. simple sequence length polymorphisms, SSLPs, single nucleotide polymorphisms), they become valuable genetic markers, i.e. loci which can be used to distinguish individuals. (wikipedia.org)
DNA isolation, genotyping of simple tandem repeat polymorphisms, PCR amplification and sequencing were performed using standard techniques 2 and all simple tandem repeat polymorphisms were amplified in the presence of 10% glycerol and 5% DMSO after labelling one oligonucleotide primer with 32 P and analysed on a 6% polyacrylamide gel. (bmj.com)
However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. (origene.com)

We show here that the multiplex genotyping of multiple mutation sites is possible, based on multiplex PCR DNA amplification and a stem-loop probe method. (hindawi.com)
In Is PETase Austin , the focus was to narrow the active site and to make it more cutinase-like by introducing the double mutation S238F and W159H, whereas the triple mutation S121E, D186H and R280A in Is PETase Son was centred around stabilizing loop regions, thereby improving the overall temperature stability. (biomedcentral.com)
The relative prevalence of each mutation in Omicron sequences in not yet known as the sequencing data at the present time is limited. (who.int)

Furthermore, it is suggested that the binding of SRP to the signal sequence is promoted by the presence of basic amino acid residues through electrostatic interactions 18 . (nature.com)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)

For this reason they are useful for constructing genetic and physical maps from sequence data reported from many different laboratories. (wikipedia.org)

To determine the actual sequence specificity to SRP, a collection of mutants was constructed. (nature.com)

These reactions produce templates that can be amplified by PCR, using universal primer sequences. (hindawi.com)
Finally, the pooling of DNA samples or multiplex PCR strategies for SNP genotyping have also been described in several microarray methods, such as the original arrayed primer extension (APEX) [ 9 - 12 ] or single base extension (SBE-tags) [ 13 , 14 ] assays. (hindawi.com)

In contrast to the abundance mutations in SDHB and SDHD , only a single multiply affected family and an isolated case, containing a single nucleotide initiation codon and a splice site mutations in SDHC , respectively, have been described by Niemann et al . (bmj.com)

Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. (lookformedical.com)

METHODS: To establish the prevalence of VAPB mutations, we screened 80 familial ALS samples by DNA sequencing. (bvsalud.org)

Sequencing of these specimens identified the new variant of SARS CoV-2 B.1.1.529, now called Omicron which has over 30 mutations in the S gene. (who.int)

DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. (lookformedical.com)

The Molecular Inversion Probe assay (Parallele Biosciences, South San Francisco, Calif, USA) [ 6 ] uses probes that can be circularized and contain two sequences complementary to regions adjacent to SNPs in the DNA target in the first step of the genotyping reaction. (hindawi.com)

Swine-specific sequence tagged (Microsatellite) sites, STS and STMS, were assigned chromosomally by polymerase chain reaction analysis of the somatic cell hybrid panel. (usda.gov)

He thanked CDC and KTL for their support in providing accurate genomic sequencing results of polioviruses in a timely manner, as this greatly facilitated planning and targeting the polio eradication activities. (who.int)

Spot signal intensities were 18 to 74 times greater for perfect matches than for mismatched target sequences differing by a single nucleotide (discrimination ratio) for "homozygous" DNA from healthy individuals. (hindawi.com)
With the ongoing developments of high throughput sequencing machines and advancement of modern bioinformatics tools at unprecedented pace, the target goal of sequencing individual genomes of living organism at a cost of $1,000 each is seemed to be realistically feasible in the near future. (springer.com)
While nine spCas9 gene editing sites surround the Arc start codon, the accuracy of the editing was highly sequence-dependent, with only a single target resulting in an in-frame reporter integration. (lu.se)

Single pass sequencing of cDNA clones provides a rapid route to gene discovery and production of EST's (2). (le.ac.uk)
Segregating populations will be characterised with both single locus (microsatellites) and multi-locus (AFLPs) marker systems providing framework maps for the eventual location of cDNAs generated from single pass sequencing. (le.ac.uk)

Find support for a specific problem in the support section of our website. (mdpi.com)

In this "SRP pathway", an open question is how a signal sequence is recognized since no consensus motif exists and is relevant for SRP binding. (nature.com)
Bugs come from flaws in the browsers' parsing of HTML -- one sequence of HTML tags can crash the application and leave it open to attack -- and its implementation of CSS (Cascading Style Sheets), which can lead to a buffer overflow and then a complete computer compromise. (designdir.net)
We show that a sequence coding for mCherry can successfully be knocked in at the 5′ end of the Arc open reading frame. (lu.se)

In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. (nature.com)
PCR screening can support the selection of specimens for sequencing which will provide definitive identification of Omicron. (who.int)

This clone was engineered to express the complete ORF with an expression tag. (origene.com)

Characterisation and mapping of expressed sequence tags or EST's to serve as anchored sequences tagged sites (STS). (le.ac.uk)

Danish vulnerability tracker Secunia tagged the overall updates -- to Firefox 1.5.0.2 and 1.08, and Sea Monkey 1.0.1 -- as "Highly critical," its second-from-the-top ranking. (designdir.net)

If so, these two closely associated hosts from the two major lineages (restriction digest types III-2 could act as reservoirs for each other and sites for the and III-3) that infect human neonates. (cdc.gov)

Within this sequence a Walker A motif is present at residues 30-38. (nature.com)

This is achieved by cleaving genomic DNA into fragments with a restriction enzyme and then introducing common adaptor sequences into the restriction products by ligation. (hindawi.com)

We do not evaluate or guarantee the accuracy of any content in this site. (lookfordiagnosis.com)

2003 ). The NHGRI has categorized new sequencing approaches into those that offer near-term and revolutionary benefits with a 100-fold cost reduction per base pair (bp) within the next five years. (springer.com)

1 Common tumour sites include the carotid body in the head and neck and adrenal and extra-adrenal paraganglia in the abdomen. (bmj.com)

These common sequences are then used as binding sites for common PCR primers [ 1 , 2 ]. (hindawi.com)

Anatomy3

Organisms3

Chemicals and Drugs28

Analytical, Diagnostic and Therapeutic Techniques and Equipment11

Phenomena and Processes33

Information Science4

Whole genome4

Genome6

Genomic sequences1

Polymorphic3

Sanger2

Proteins7

Nucleotide Sequence1

Shotgun1

Genes5

Chromosomes3

Protein3

Nucleotides1

Polymorphisms3

Mutation3

Amino acid2

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Primer2

Abundance1

Eukaryotes1

Methods1

Mutations1

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Polymerase1

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