Sequence situ localization of specific rnas. Medical search. Frequent questions

We here present a micrometer-scale implementation of fluorescence in situ hybridization that we term μFISH. (springer.com)
It relies on the sequence-specific hybridization of probes to their complementary targets in individual cells, followed by direct or indirect detection of the labelled probe. (springer.com)
A key milestone in ISH-based techniques was fluorescence in situ hybridization (FISH) (Bauman et al. (springer.com)
The subcellular localization and stage-specific expression of several putative lncRNAs were validated using RNA fluorescence in situ hybridization (RNA-FISH). (biorxiv.org)
However, here using reverse transcription-PCR and in situ hybridization we show that mRNA encoding the ρ1 subunit is highly expressed in brainstem neurons. (jneurosci.org)
They are microdissection techniques, Fluorescent in situ hybridization methods, in situ sequencing, in situ capture protocols and in silico approaches. (wikipedia.org)
Fluorescence in situ hybridization fish is a molecular biology technique that can be used to detect microorganisms known to biodegrade contaminants. (web.app)
Fluorescence in situ hybridization fish assays employ fluorescently labeled probes to interrogate the bcr and abl1 loci. (web.app)
Fluorescence in situ hybridization fish is an effective technique that enables direct visualization of genetic alterations in the cell. (web.app)
Fluorescence in situ hybridization fish is a cytogenic technique used for the detection and localization of rna sequences within tissues or cells 116. (web.app)
Fluorescent in situ hybridization fish is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a. (web.app)
Fluorescent in situ hybridization fish assay grenoble ecobiz. (web.app)
Fluorescence in situ hybridization fish is a technique that uses fluorescent probes which bind to special sites of the chromosome with a high degree of sequence complementarity to the probes. (web.app)
Fluorescence in situ hybridization fish preparation of fish probe recommended filter set fish is a technique used to identify and localize the presence or absence of specific dna sequences on cells and tissues. (web.app)
It has emerged as a powerful cytogenetic method for the analysis of cells and tissues on the transcriptome and genome level with more than 6 publications in the literature per day since the late 1990s fig. Fluorescence in situ hybridization fact sheet itrc. (web.app)
Why is fluorescence in situ hybridization relevant. (web.app)
Fluorescence in situ hybridization fish is a cytogenetic technique used to detect the presence or absence and location of specific gene sequences. (web.app)
Review applications of fluorescence in situ hybridization fish in. (web.app)
The application of fluorescence in situ hybridization fish to cll has substantially enhanced our ability to detect chromosomal aberrations. (web.app)
Fluorescence in situ hybridization fish preparation of fish probe recommended filter set. (web.app)
The development of molecular hybridization techniques such as fluorescence in situ hybridization fish has had a major impact on efforts to detect and characterize the genetic changes that give. (web.app)
Fluorescence in situ hybridization fish is a molecular cytogenetic technique that uses. (web.app)
Pdf the development of molecular hybridization techniques such as fluorescence in situ hybridization fish has had a major impact on. (web.app)
Procedures for fluorescent in situ hybridization materials supplied directly labeled probe in hybridization buffer green or orange depending on the kit type storage instruction store at 20c in the dark. (web.app)
See the in situ hybridization pattern in Kohara NextDB . (nih.gov)
We detected TSPyV RNA in tissue specimens by in situ hybridization, which revealed localization to endothelial cells. (cdc.gov)
We performed in situ hybridization using a custom RNAScope probe that targeted the complete TSPyV viral genome (GenBank accession no. (cdc.gov)
For negative in situ hybridization controls, we used tissue specimens from patients with Merkel cell polyomavirus-positive Merkel cell carcinoma and BK polyomavirus nephropathy and a T-cell lymphoma tissue microarray that included 1 case of Merkel cell polyomavirus-positive T-cell lymphoma. (cdc.gov)
We performed hematoxylin and eosin (H&E) staining and RNAScope in situ hybridization (ISH) to detect trichodysplasia spinulosa. (cdc.gov)
Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
Alternatively spliced lncRNAs were detected with in situ hybridization with variant-specific RNA probes on human cornea sections. (molvis.org)
Finally, in situ hybridization analyses revealed the presence of RP11-295G20.2 in the nuclei of corneal epithelial cells, but not in the stromal cells of the human cornea, while RP11-322M19.1 was present in epithelial and non-epithelial cells. (molvis.org)
Therefore, we analyzed the potential of fluorescence in situ hybridization (FISH) for diagnosing WD, using a Tropheryma (T.) whipplei -specific probe. (frontiersin.org)
The expression levels of FENDRR in hypoxic pulmonary artery endothelial cells (HPAECs) were detected by using quantitative real-time polymerase chain reaction (qRT-PCR) and fluorescence in situ hybridization (FISH). (biomedcentral.com)
Individual expression patterns are extraordinarily diverse, but by supplementing qualitative in situ hybridization data with quantitative microarray time-course data using a hybrid clustering strategy, we identify groups of genes with similar expression. (biomedcentral.com)
RNA in situ hybridization has the potential to reveal both spatial and temporal aspects of gene expression during development. (biomedcentral.com)
However, RNA in situ hybridization is not quantitative [ 7 ]. (biomedcentral.com)
The probes are great for in situ hybridization and cell or tissue imaging applications requiring a short probe, such as in the detection of short sequence mutations or for discriminating between closely related sequences such as splice variants and isoforms. (qiagen.com)
And their high affinity for their target sequences allows use of stringent hybridization and washing conditions, despite the short probe lengths. (qiagen.com)
This GLABR gene was further characterized by a conditional knockout experiment as well as an in situ hybridization on sectioned epithelial cells in order to better determine its function. (ubc.ca)
RNA pull down assay, luciferase reporter assay and fluorescence in situ hybridization were applied to verify the interaction between BCRC-3 and microRNAs. (biomedcentral.com)
Localization of HuC (ELAVL3) to chromosome 19p13.2 by fluorescence in situ hybridization utilizing a novel tyramide labeling technique. (nih.gov)
RT-PCR and in situ hybridization (ISH) demonstrated that granulosa cells express wnt-2 mRNA. (elsevierpure.com)
In general, spatial resolution transcriptomics is mainly divided into two categories: One is an image-based method based on in situ hybridization, in which staining and barcoding RNA in its original position are the key points to achieve multiple detection. (encyclopedia.pub)
Image-based methods originating from single-molecule RNA in situ hybridization are mainly divided into fluorescence in situ hybridization and fluorescence in situ sequencing. (encyclopedia.pub)
Fluorescence in situ hybridization uses labeled fluorescent probes to determine RNA/DNA abundance in tissues or cells, and the first example of imaging a single RNA species can be attributed to single-molecule fluorescence in situ hybridization (smFISH) [ 12 ] , which provides absolute quantification of the copy number and localization of RNA molecules in cells. (encyclopedia.pub)
NEW YORK, NY, Sept. 26, 2022 (GLOBE NEWSWIRE) -- Enzo Biochem, Inc. (NYSE: ENZ) ("Enzo" or the "Company"), a leading biosciences and diagnostics company, today announced the launch of the AMPIVIEW™ line, a breakthrough RNA gene expression platform powered by Enzo's LoopRNA™ in situ hybridization technology. (enzo.com)
HPV testing is part of Enzo's focus on women's health and oncology and leverages the Company's long history in HPV detection while complementing the existing offering of Enzo's POLYVIEW® immunohistochemistry ("IHC") and PATHO-GENE® in situ hybridization ("ISH") detection platforms, an enhanced diagnostic detection system used by pathologists in reading tissue biopsies. (enzo.com)
Mechanistically, dual luciferase reporter assays, fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP) and RNA pull-down experiments were performed to confirm the underlying mechanisms of circHERC1. (biomedcentral.com)

Spatial transcriptomics is a method for assigning cell types (identified by the mRNA readouts) to their locations in the histological sections and can also be used to determine subcellular localization of mRNA molecules. (wikipedia.org)
Then, streptavidin capture of the biotin group is used to extract poly(A)-tailed mRNA molecules bound to unblocked tags, after which these mRNAs are analyzed by RNA sequencing. (wikipedia.org)
To address the mechanism of isoform-specific mRNA localization, molecular chimeras were constructed by insertion of actin sequences between the Lac Z coding region and SV-40 3'UTR in a reporter plasmid. (umassmed.edu)
Since the blue-insoluble reaction product co-localized with the specific mRNAs expressed from each construct, it was used as a bioassay for mRNA localization. (umassmed.edu)
We propose that the mechanism of mRNA localization facilitates actin isoform sorting in the cytoplasm. (umassmed.edu)
In situ localization of HLA class I mRNA in human testis. (ox.ac.uk)
We have used a 0.35-kilobase (kb) antisense RNA probe complementary to the monomorphic regions of both classical and nonclassical HLA class I sequences to detect histocompatibility-class-I-antigen-specific mRNA in human testicular tissue. (ox.ac.uk)
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. (nih.gov)
Our Custom LNA mRNA Detection Probes are designed with optimal LNA positioning to achieve high sequence specificity, low secondary structure and minimal self-annealing. (qiagen.com)
Our user-friendly online design tool lets you easily design highly sensitive LNA probes for your specific mRNA sequence of interest. (qiagen.com)
This lack of increase contrasts with previous reports as does the mRNA length: this is 50% larger as confirmed by sequencing the 3' end. (embl.de)
Zu Beginn der Arbeit sollten Methoden für die exakte Identifizierung der beteiligten regulatorischen RNA-Sequenzen und der miRNA-mRNA Interaktionen entwickelt werden. (fu-berlin.de)
The first aim of the study was to develop methods for the efficient and accurate identification of the involved regulatory RNA sequences and the miRNA-mRNA interactions. (fu-berlin.de)

Additionally, the genome-wide occupancy of several candidate nuclear lncRNAs was explored using Chromatin Isolation by RNA Purification (ChIRP). (biorxiv.org)
The relationship between FENDRR and dynamin-related protein 1 (DRP1) was explored using bioinformatics analysis, Chromatin Isolation by RNA Purification (CHIRP), Electrophoretic mobility shift assay (EMSA) and Methylation-Specific PCR (MSP) assays. (biomedcentral.com)

Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
RNA-targeting CRISPR-Cas systems - We use biochemical activity assays in conjunction with structural techniques and sequencing to investigate RNA-targeting CRISPR systems, such as the Type VI effector, Cas13a. (berkeley.edu)
The interaction of circHERC1 with FOXO1 was verified by RNA pull-down, RNA immunoprecipitation (RIP) and western blot assays. (biomedcentral.com)

In theory, any nucleic acid can be used as a probe provided it can be labeled to permit detection and quantitation of the hybrid molecules formed between the probe and sequence to be identified. (enzolifesciences.com)
RNA: Fluorochrome can detect RNA molecules, allowing researchers to study gene expression at the transcriptional level. (dadangoray.com)
They can be used to label and identify specific molecules or structures within cells or tissues. (dadangoray.com)
Long noncoding RNAs (lncRNAs) are a large class of RNA molecules ranging in length from 200 to 100,000 nt and located in the nucleus or cytoplasm (Ponting et al. (biomedcentral.com)
RNA molecules are uniquely capable of encoding and controlling the expression of genetic information, often as a consequence of their three-dimensional structures. (berkeley.edu)
Noncoding RNAs (ncRNAs) are a large and diverse class of transcribed RNA molecules and participate in many biological processes [ 3 ]. (biomedcentral.com)

An advanced alternative for RNA Sequencing of Individual Cryosections described above, RNA tomography (tomo-seq) features better RNA quantification and spatial resolution. (wikipedia.org)
The sequence analyses and quantification of splice variants of candidate lncRNAs, including RP11-295B20.2, RP11-18I14.1, and RP11-322M19.1, demonstrated complex configuration of their splicing changes, with a significant impact of PNN on the process. (molvis.org)

Therefore, 3'UTR sequences play a key role in modulating the distribution of actin mRNAs in muscle cells. (umassmed.edu)
They can bind to the 3′ untranslated region (3′UTR) of target messenger RNAs (mRNAs) in order to regulate gene expression post-transcriptionally with no sequence specificity but by potential electrostatic interactions with the phosphate backbone of RNA 2 , 3 . (nature.com)
In practice, double and single stranded DNAs, mRNAs, and other RNAs synthesized in vitro are all used as probes. (enzolifesciences.com)
Some circRNAs contain miRNA response elements (MREs) and function as competing endogenous RNAs (ceRNAs) to interact with miRNAs and regulate the expression of target mRNAs. (biomedcentral.com)

Expressed sequence tag (EST) analysis and developmental polymerase chain reaction (PCR) studies demonstrated that Ov-phy-1 was expressed in L3 and adult parasites. (embl.de)

Circular RNAs (circRNAs) are regulatory RNAs which have recently been shown to have clinical significance in several diseases, including, but not limited to, various cancers, neurological diseases and cardiovascular diseases. (sciencegate.app)
Circular RNAs (circRNAs) are a new member of noncoding RNAs (ncRNAs) that have recently been described as key regulators of gene expression. (biomedcentral.com)
Circular RNAs (circRNAs), a new member of noncoding RNAs (ncRNAs), have attracted great attentions for their closed continuous loop structure and potential value in clinical work [ 9 , 10 ]. (biomedcentral.com)
Noncoding RNAs such as circular RNAs (circRNAs) are abundant in the human body and influence the occurrence and development of various diseases. (biomedcentral.com)

Fluorochrome detection refers to a technique used to identify and quantify specific biomolecules using fluorescent dyes. (dadangoray.com)
Fluorochrome detection involves using a specific dye that is excited by light of a particular wavelength to emit light at a different wavelength. (dadangoray.com)
In summary, fluorochrome detection is a powerful technique used to identify and quantify specific biomolecules in cells and tissues. (dadangoray.com)
This gives you superior binding affinity and very specific and sensitive detection, with an exceptionally high signal-to-noise ratio. (qiagen.com)
INTERPRETATION: This investigation substantiates the use of metagenomic next-generation sequencing for the broad-based detection of rare or unexpected pathogens. (cdc.gov)
This innovative design enables sensitive and specific detection of target genes in tissue or cell samples while preserving the morphology. (enzo.com)

Currently, a range of probes can be synthesized to locate and quantify specific short RNAs, genes, entire chromosomes, and even cells (Evanko 2007 ). (springer.com)
In Vertebrates, Nanos paralogous genes were associated to partial redundancies and specific functional evolutions. (nature.com)
In teleostean fish, two nanos1 genes were generated, probably during the Teleost specific whole genome duplication (3R). (nature.com)
Swr1 is required for the deposition of histone H2AZ at specific chromosome locations in vivo, and Swr1 and H2AZ commonly regulate a subset of yeast genes. (sdbonline.org)

In Paper II , we present an assay for spatial RNA quality evaluation, used to estimate the success for tissue specimens before proceeding with more expensive spatial sequencing methods. (kth.se)

By emitting fluorescent light when exposed to specific wavelengths of light, fluorochromes have become indispensable tools in the fields of biology and medicine. (dadangoray.com)

These systems are tested for function in vitro , heterologously in vivo , in situ microbial communities, and in eukaryotic cells. (berkeley.edu)
Deconvolution of spatial sequencing provides accurate characterization of hESC-derived DA transplants in vivo. (lu.se)

This method allows further RNA transcript profiling and cDNA library generation of the retrieved cells. (wikipedia.org)
In Paper IV , we present an approach to investigate in situ transcript derivedinferred copy number variation (iCNV) profiles based on spatial transcriptomics data. (kth.se)

A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)

Besides oligo(dT) tail and spatial barcode, which indicates the x and y position on the arrayed slide, the probe contains a cleavage site, amplification and sequencing handle, and unique molecular identifier. (wikipedia.org)
We used the RNAscope 2.5 LS Probe-Hs-PPIB-sense probe (Advanced Cell Diagnostics), which is specific for peptidylprolyl isomerase B, to demonstrate the presence of intact RNA in the tissue sections. (cdc.gov)
In developing a probe, a sequence of nucleotides must be identified, isolated, reproduced in sufficient quantity, and tagged with a label that can be detected. (enzolifesciences.com)
In collaboration with the laboratory of David Savage , we have also developed a series of high-throughput mutant library construction, screening, and sequencing pipelines to probe CRISPR-Cas protein function and to isolate engineered variants with novel properties. (berkeley.edu)

In vitro production of enzymatically active O. volvulus prolyl 4-hydroxylase should facilitate identification of specific inhibitors of the parasite enzyme. (embl.de)

Proteins supported by a single continuous cDNA sequence lead to underlining the name/ending of the variant. (nih.gov)
Nanos are RNA-binding proteins playing crucial roles in germ cell development and maintenance. (nature.com)
The Nanos proteins family regroups highly conserved RNA-binding proteins in higher eukaryotes implicated in germ cell development and maintenance. (nature.com)
They can detect everything from DNA and RNA to proteins and lipids, allowing researchers to investigate a vast array of cellular functions. (dadangoray.com)
Proteins: Fluorochrome can detect specific proteins in cells or tissues, allowing researchers to study their distribution and expression levels. (dadangoray.com)
The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. (nih.gov)

Specific molecular probes and primers are designed for this purpose. (enzolifesciences.com)
Random priming is a type of primer extension in which a mixture of small oligonucleotide sequences, acting as primers, anneal to a heat-denatured double-stranded template. (enzolifesciences.com)
Using RT-PCR with degenerate primers on RNA from ovaries of hormone-stimulated immature rats, we identified transcripts for wnt-2 and wnt-2b. (elsevierpure.com)
RT-PCR analysis, using primers designed from this wnt-2b cDNA sequence, failed to detect transcripts in the ovarian follicular compartment (granulosa and oocyte). (elsevierpure.com)
RT-PCR, using degenerate and specific primers for wnts, on RNA from five ovarian cancer cell lines confirmed the expression of transcripts for wnt-2b. (elsevierpure.com)
The second category is based on in situ capture and sequencing: the spatial information is marked by barcoded primers attached to a solid surface, and then the spatial information is retrieved by high-throughput sequencing. (encyclopedia.pub)
We verified this excision event using primers (not shown) to amplify the 3' arm and sequencing through the remaining FRT site. (cdc.gov)

Knockdown of PNN in HCET cells led to specific changes in the inclusion of multiple cassette exons as well as in the use of alternative splice sites in RP11-322M19.1 and RP11-18I14.1, resulting in considerable net changes in the ratio between the splice variants. (molvis.org)

This was revealed by co-hybridizing, in situ, fluorochrome-conjugated oligonucleotide probes specific for each isoform. (umassmed.edu)
Molecular probes can be broadly categorized into DNA probes and RNA probes, cDNA probes, and synthetic oligonucleotide probes. (enzolifesciences.com)

High-throughput sequencing methods are utilized to analyze the mutational spectrum and evolutionary trajectories of tumor cells. (spandidos-publications.com)

Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
Prokaryotes have evolved a nucleic acid-based immune system that shares some functional similarities with RNA interference in eukaryotes. (berkeley.edu)

Further mechanistic research indicated that circHERC1 acted as a competing endogenous RNA of miR-142-3p to relieve the repressive effect of miR-142-3p on its target HMGB1, activating the MAPK/ERK and NF-κB pathways and promoting cell migration and invasion. (biomedcentral.com)

RNA sequencing of the selected regions in individual cryosections is another method that can produce location-based genome-wide expression data. (wikipedia.org)
It is also based on tissue cryosectioning with further RNA sequencing of individual sections, yielding genome-wide expression data and preserving spatial information. (wikipedia.org)

RNA immunoprecipitation (RIP) and m6A dot blot were used to detect the m6A modification levels of FENDRR. (biomedcentral.com)

Polyglutamine-binding protein 1 ( PQBP1 ) is an RNA-splicing factor that, when mutated, in humans causes Renpenning syndrome, an X-linked intellectual disability disease characterized by severe cognitive impairment, but also by physical defects that suggest PQBP1 has broader functions in embryonic development. (xenbase.org)
To begin, we hypothesize that distinct morphological features, in other words, hairiness are a result of Wookiee specific protein-coding gene(s). (ubc.ca)
A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. (nih.gov)
Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis. (nih.gov)
Lipid-specific oligomerization of the Marburg virus matrix protein VP40 is regulated by two distinct interfaces for virion assembly. (uci.edu)
These systems can be extremely divergent mechanistically and provide a rich area to research RNA:protein interactions, including novel protein folds. (berkeley.edu)
In this platform, catalytically inactive Cas9 serves as an RNA-guided DNA binding protein to silence gene expression in a sequence-specific manner. (berkeley.edu)

Microglia cells have been implicated to play an important role in the tumor microenvironment, and we found spatial co-localization of microglia and epithelial-to-mesenchymal transition (EMT) signatures in our patient cohort. (kth.se)

We showed that the method is capable of measuring high RNA quality in tissue areas of both high and low cell density and that the spatial RNA integrity patterns are reflected in spatial transcriptomics data. (kth.se)

A dualcolor, dualfusion approach is commonly used and can detect all bcrabl1 fusions with excellent sensitivity and specificity including cytogenetically cryptic translocations fig. Fish is a cytogenetic technique used to detect and localize the presence or absence of specific. (web.app)
Fish is a technique used to identify and localize the presence or absence of specific dna sequences on cells and tissues. (web.app)
In contrast, sequences from the 5'UTR through the coding region of either actin gene did not localize the blue product. (umassmed.edu)
In contrast to our hypothesis, we found that TSPyV RNA did not localize to neoplastic cells. (cdc.gov)

Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)
Meanwhile, it has unique advantages in the analysis of specific gene expression and cell localization and can provide key information for the research of detailed molecular mechanisms. (encyclopedia.pub)
Our results uncover the structural basis for foreign DNA capture and the mechanism by which Cas1-Cas2 functions as a molecular ruler to dictate the sequence architecture of CRISPR loci. (berkeley.edu)

This problem was resolved by adding RNA of a distantly related Drosophila species to each tube after initial RNA extraction. (wikipedia.org)

This affinity and complementary sequence allow binding to specific regions of a target sequence of nucleotides. (enzolifesciences.com)

ChIRP-seq of candidate lncRNAs revealed that lncRNA occupancy sites within the parasite genome are focal and sequence-specific with a particular enrichment for several parasite-specific gene families, including those involved in pathogenesis, erythrocyte remodeling, and regulation of sexual differentiation. (biorxiv.org)

We employ traditional biochemistry, combined with sequencing, live-cell imaging, and structural biology approaches, to uncover the fundamental mechanisms driving the CRISPR effectors now being developed as genome editing and diagnostic tools. (berkeley.edu)

Thanks to the expression of specific receptors, tip cells can sense the microenvironment, and respond to guidance cues to lead the angiogenic sprout. (elifesciences.org)

We tested various specimens (blood, cerebrospinal fluid, intraocular fluid, serum, and tissues) from the organ donor and recipients by serology, RT-PCR, immunohistochemistry, metagenomic next-generation sequencing, and host gene expression, and conducted a traceback of blood transfusions received by the organ donor. (cdc.gov)

Profiling 2 to 4 single cells per patient and measuring gene-specific expression from targets previously associated with metastatic breast cancer supports the use of our protocol as a diagnostic tool. (kth.se)

The BK polyomavirus nephropathy sections showed weak, orange-red discoloration of tubular epithelium ( Figure , panel L) that was distinguishable from the bright red, granular staining patterns for TSPyV RNA observed in the positive control and tissue specimens from the 3 patients. (cdc.gov)
APOE ε4 genotype further modified the strength of the associations with different patterns, depending on specific marker and type of dementia. (sciencegate.app)

ISH is performed without isolation of the targets from their source, i.e., in situ , and is widely used in research and diagnostics. (springer.com)
Nucleocytoplasmic localization of FOXO1 was determined by nucleocytoplasmic isolation and immunofluorescence. (biomedcentral.com)

However, this cascade of transcripts is unlikely to be regulated by the limited number of identified parasite-specific transcription factors. (biorxiv.org)
Emerging evidence suggests that N6-methyladenosine (m6A)-modified transcripts of long noncoding RNAs (lncRNAs) are important regulators that participate in many diseases. (biomedcentral.com)
The localization of these transcripts in specific ovarian compartments suggests that this signal transduction pathway may be involved in follicular development and ovarian function. (elsevierpure.com)

Results Using one of our primary models, we show that our method accurately and rapidly classifies human and mouse sequences from nine different sequencing libraries by species, library and both together, 98.32%, 97.86% and 96.38% of the time, respectively. (ghenry.info)
Furthermore, other near-human species such as Zeltron, Chiss , and Sullustan share the human ortholog of this locus, which supports the inference that the ancestral sequence was noncoding, and that the GLABR has de novo origins in the Wookiee. (ubc.ca)
The channel inventories probably reflect adaptations to species-specific challenges during fertilization. (elifesciences.org)

NC_014361.1) and RNAscope 2.5 HD Reagent Kit-RED (Advanced Cell Diagnostics, https://acdbio.com ) to detect TSPyV RNA in cutaneous biopsy specimens. (cdc.gov)

Nanos family is characterized by two specific Cys-Cys-His-Cys zinc finger motifs at the carboxy-terminal region which present a regular spacing between the Cys and the His residues. (nature.com)

2019 ). However, the reported lncRNAs in HPH are all focused on the cytoplasm of PASMCs, and the specific lncRNAs located in the nucleus, especially in PAECs involved in HPH progression and their related regulatory mechanism, remain largely unknown. (biomedcentral.com)

We further validated the function of one specific lncRNA (lncRNA-ch14) using the CRISPR-Cas9 genome editing tool. (biorxiv.org)

Additionally, DRP1 is a downstream target gene of FENDRR, and FENDRR formed an RNA-DNA triplex with the promoter of DRP1, which led to an increase in DRP1 promoter methylation that decreased the transcriptional level of DRP1. (biomedcentral.com)

We had analyzed circRNA high-throughout sequencing from human tissues and determined bladder cancer related circRNA-3 (BCRC-3, GenBank: KU921434.1) as a new candidate circRNA derived from PSMD1 gene. (biomedcentral.com)
High-throughput sequencing was used to analyze differential circRNA expression in patients with lung cancer. (biomedcentral.com)

Recent work has demonstrated the role of CRISPRs in adaptive immunity and shown that small RNAs derived from CRISPRs (crRNAs) are implemented as homing oligos for the targeted interference of foreign DNA. (berkeley.edu)

The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)

It can visualize specific cytogenetic abnormalities copy number aberrations such as chromosomal deletion, amplification, and translocation. (web.app)

There are numerous examples of ocular surface diseases in which the corneal-specific epithelial qualities are not maintained and significant anterior eye physiologic perturbations occur resulting in dramatic loss of vision. (molvis.org)

Anatomy39

Organisms21

Diseases1

Chemicals and Drugs85

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Psychiatry and Psychology1

Phenomena and Processes72

Disciplines and Occupations3

Information Science4

Hybridization50

MRNA13

Chromatin Isolation by RNA Purification2

Assays5

Molecules6

Quantification2

MRNAs4

Polymerase chain re1

Circular RNAs4

Detection6

Genes4

Assay1

Fluorescent1

Vivo2

Transcript2

Nucleotide sequence1

Probe4

Vitro1

Proteins6

Primers7

Splice variants1

Oligonucleotide Probes2

Methods1

Nucleic2

Endogenous1

Genome-wide2

Immunoprecipitation1

Protein7

Tumor microenvironment1

Spatial transcriptomics1

Localize4

Molecular3

Distantly related1

Complementary1

ChIRP1

Approaches1

Microenvironment1

Immunohistochemistry1

Gene-specific1

Patterns2

Isolation2

Transcripts3

Species3

Biopsy specimens1

Motifs1

Regulatory1

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Additionally1

CircRNA2

Interference1

Isoform1

Cytogenetic1

Diseases1