• However, a Borealin homologue has never been characterized in the fission yeast, Schizosaccharomyces pombe. (uky.edu)
  • Thus, this study characterizes a fission yeast Borealin homologue and reveals a previously unrecognized connection between the CPC and the process of cytokinesis in S. pombe. (uky.edu)
  • We have sequenced and annotated the genome of fission yeast (Schizosaccharomyces pombe), which contains the smallest number of protein-coding genes yet recorded for a eukaryote: 4,824. (inrae.fr)
  • When expressed in S. cerevisiae , the S. pombe Mdm12p homolog conferred a dominant-negative phenotype of giant mitochondria and aberrant mitochondrial distribution, suggesting partial functional conservation of Mdm12p activity between budding and fission yeast. (rupress.org)
  • The fission yeast S. pombe lacks Sir3p and Sir4p but employs methylation of histone H3 lysine 9 (K9) plus a Sir2p homologue (Sir2) for silencing [ 15 ]. (biomedcentral.com)
  • Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha , which mate to form viable hybrid diploids that efficiently complete meiosis, but generate few viable gametes. (elifesciences.org)
  • have now looked at two species of fission yeast- S. pombe and S. kambucha -that share 99.5% of their DNA sequence. (elifesciences.org)
  • Mitochondrial ribosomal proteins were studied best in yeast, where the small subunit was shown to contain about 35 proteins. (pasteur.fr)
  • Using a recently described affinity purification technique and tagged versions of yeast Ykl155c and Mrp1, we isolated this mitochondrial ribosomal subunit and identified a total of 20 proteins, of which 12 are new. (pasteur.fr)
  • For a subset of the newly described ribosomal proteins, we showed that they are localized in mitochondria and are required for the respiratory competency of the yeast cells. (pasteur.fr)
  • The C-terminal domain shows strong sequence similarity to human, frog, and yeast Orc4 proteins, including conserved ATP-binding motifs. (embl.de)
  • Homologues of the yeast Oxa1 protein are found in chloroplasts of plants and in a wide variety of bacteria. (tcdb.org)
  • Nonconventional yeast species are attracting attention due to their unique properties and as a potential source of genes that encode proteins with unusual characteristics. (bvsalud.org)
  • The FH1 domain is also a binding site for diverse SH3-domain containing proteins like Src-like non-receptor tyrosine kinases, WISH (WASP-interacting SH3 protein) and IRSp53 (insulin receptor substrate) in mammals, and Hof1p in yeast [ 6 ]. (biomedcentral.com)
  • Yet, genetic and biochemical studies identified only 14 proteins, half of which were predictable by sequence homology with prokaryotic ribosomal components of the small subunit. (pasteur.fr)
  • Pairwise blast analysis using the clustal omega program revealed strong homology between the mouse and human prmt7 sequences (85. (shopambitionhustle.com)
  • Formins are multidomain proteins defined by a conserved FH2 (formin homology 2) domain with actin nucleation activity preceded by a proline-rich FH1 (formin homology 1) domain. (biomedcentral.com)
  • Annotation transfer for function and structure within the sequence homology concept essentially requires protein sequence similarity for the secondary structural blocks forming the fold of a protein. (beds.ac.uk)
  • Based on our previous work of dissecting the hidden markov model (HMMER) based similarity score into fold-critical and the non-globular contributions to improve homology inference, we propose a framework-dissectHMMER, that identifies more fold-related domain hits from standard HMMER searches. (beds.ac.uk)
  • The proposed framework-dissectHMMER, is faithful to the original inception of the sequence homology concept while improving upon the existing HMMER search tool through the rescue of statistically evaluated false-negative yet fold-related domain hits to the query sequence. (beds.ac.uk)
  • The sequence homology concept [ 1 - 3 ] is collectively founded upon the inductive reasoning that a homologous protein group (as an antecedent) shares a high level of sequence similarity (as a consequent) [ 4 - 8 ]. (beds.ac.uk)
  • Despite the simplicity and elegance of the sequence homology concept, homology itself is not readily computable. (beds.ac.uk)
  • This measure comes along with the caveat of possibly high sequence similarity does not necessarily imply homology. (beds.ac.uk)
  • Worse, as we delve deeper into sequence search space, high sequence divergence among the distant homologs will inevitably corrupt the homology signal. (beds.ac.uk)
  • CENP-H shows sequence similarity to the Schizosaccharomyces pombe kinetochore protein Fta3 which is a subunit of the Sim4 complex. (wikipedia.org)
  • Nbl1 (novel Borealin-like 1) physically associates with known CPC components, affects the kinase activity and stability of the S. pombe Aurora B homologue, Ark1, colocalizes with known CPC subunits during mitosis, and shows sequence similarity to human Borealin. (uky.edu)
  • This brings to 26 the total number of proteins described as components of the mitochondrial small ribosomal subunit. (pasteur.fr)
  • We have characterized a Schizosaccharomyces pombe homologue of the ORC subunit, Orc4p. (embl.de)
  • This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. (icbinhibitor.com)
  • The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. (icbinhibitor.com)
  • The genomic sequence provides access to the complete structures of all genes, including those without known function, their control elements, and, by inference, the proteins they encode, as well as all other biologically important sequences. (inrae.fr)
  • Sequences and the proteins they encode. (gpiaca.com)
  • claiming that two homologous chromosomes paired between complementary sequences lead to the formation of a cross-stranded structure that physically links the two component helices. (iucr.org)
  • the filamentous fungus aspergillus oryzae is a well-known expression host used to express homologous and heterologous proteins in a number of industrial applications. (liverpool.ac.uk)
  • Implicitly, this refers to a high level of similarity among comparable structural elements across the sequences so that a common structural fold among these homologs is maintained which, in turn, governs the general biological function of this homologous protein family. (beds.ac.uk)
  • As such, statistically significant yet spurious alignments (attributed by the remnant segments) can pass off as homologous sequences once they escape the designated statistical threshold. (beds.ac.uk)
  • In more distant species, putative homologues were detected for Ykl155c, which shares conserved motifs with uncharacterized proteins of higher eukaryotes including humans. (pasteur.fr)
  • Two genes encoding putative internalin proteins were chosen for further study. (omictools.com)
  • Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for STB5 . (yeastrc.org)
  • Transferring the functional annotation of a protein to its orthologs in other species is therefore routine in genome annotation. (biomedcentral.com)
  • To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. (biomedcentral.com)
  • Mdm12p is the third mitochondrial outer membrane protein required for normal mitochondrial morphology and distribution to be identified in S. cerevisiae and the first such mitochondrial component that is conserved between two different species. (rupress.org)
  • The re-annotation also utilises sequence data from additional Campylobacter strains and species not available during the original annotation. (omictools.com)
  • MMDB and VAST+: tracking structural similarities between macromolecular complexes. (nih.gov)
  • One of these components, the Mdm1p protein, is a cytoskeletal element that exhibits structural similarities to the intermediate filament proteins of animal cells ( McConnell and Yaffe, 1992 , 1993 ). (rupress.org)
  • SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
  • Hence, the only recourse to maintain on the correct search path is to piggyback on the similarity between the structural pieces of the alignment to ensure reasonable fold similarity and, hence, the implied biological function. (beds.ac.uk)
  • Regrettably, current implementation of sequence search algorithms do not consciously differentiate between the 3D-structural (i.e., fold-critical) sequence segments and the non-globular (i.e., remnant) segments. (beds.ac.uk)
  • The ScTrk1 protein can be of great interest to industry, as it was shown that changes in its activity influence ethanol production and tolerance in S. cerevisiae and also cell performance in the presence of organic acids or high ammonium under low K+ conditions. (bvsalud.org)
  • The S. cerevisiae Mdm12p was localized by indirect immunofluorescence microscopy and by subcellular fractionation and immunodetection to the mitochondrial outer membrane and displayed biochemical properties of an integral membrane protein. (rupress.org)
  • Telomeric silencing has been demonstrated in organisms ranging from yeasts to humans (reviewed in [ 9 ]) but it has been best characterized in S. cerevisiae , Schizosaccharomyces pombe and Drosophila melanogaster , organisms that have no, or very little, DNA methylation. (biomedcentral.com)
  • Mitochondrial TAT transports folded proteins in those eukaryotes with TatA and TatC subunits encoded in the mitochondrial genome. (tcdb.org)
  • The N- glycosylation is an essential protein modification taking place in the membranes of the endoplasmic reticulum (ER) in eukaryotes and the plasma membranes in archaea. (biomedcentral.com)
  • The problem is solved in most eukaryotes by the addition of repeated sequences to the chromosome ends [ 1 ]. (biomedcentral.com)
  • The N-terminal domain contains nine copies of the AT-hook motif found in a number of DNA-binding proteins, including the members of the HMG-I(Y) family of chromatin proteins. (embl.de)
  • Furthermore, AT-hook motifs are frequently associated with known functional domains seen in chromatin proteins and in DNA-binding proteins (e.g. histone folds, homeodomains and zinc fingers). (embl.de)
  • The DNA in the cell is packaged with histone proteins into chromatin. (usc.edu)
  • In plants and Schizosaccharomyces pombe , small interfering RNA (siRNA)-directed chromatin modifications (DNA methylation for plants and H3K9 methylation [H3K9me] for S. pombe ) lead to transcriptional repression. (biomedcentral.com)
  • Despite these similarities, several features of nuclear RNAi in C. elegans make this pathway a unique model system to explore novel mechanisms of RNA-mediated chromatin silencing and its roles in developmental regulation. (biomedcentral.com)
  • Mex67p of Schizosaccharomyces pombe interacts with Rae1p in mediating mRNA export. (ox.ac.uk)
  • In vitro, the FH2 domain competes with barbed-end capping proteins and is necessary and sufficient to nucleate actin polymerization, but the FH1 domain, which interacts with profilin-actin, funnels actin to the nucleation vicinity and confers full activity to the molecule [ 1 ]. (biomedcentral.com)
  • Here, we isolate a previously uncharacterized S. pombe protein through association with the Cdc14 phosphatase homologue, Clp1/Flp1, and identify it as a Borealin-like member of the CPC. (uky.edu)
  • The genome of C. albicans strain WO-1, sequenced at the Broad Institute as part of the Fungal Genome Initiative (FGI). (candidagenome.org)
  • Small DNA-binding motif first described in the high mobility group non-histone chromosomal protein HMG-I(Y). (embl.de)
  • The AT-hook is a small DNA-binding protein motif which was first described in the high mobility group non-histone chromosomal protein HMG-I(Y). Since its discovery, this motif has been observed in other DNA-binding proteins from a wide range of organisms. (embl.de)
  • Post-translational modification of proteins at lysine residues by reversible acetylation is catalyzed by the opposing activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs), which act on both histone and non-histone substrates despite their names. (biomedcentral.com)
  • dim-5 , which encodes a histone H3 K9 methyltransferase and hpo , which encodes heterochromatin protein-1. (biomedcentral.com)
  • Silent information regulator 2 (Sir2) proteins, or sirtuins, are protein deacetylases dependent on nicotine adenine dinucleotide (NAD) and are found in organisms ranging from bacteria to humans. (biomedcentral.com)
  • The comparison of nucleotide or protein sequences from the same or different organisms is a very powerful tool in molecular biology. (gpiaca.com)
  • Cytosine C5 methylation is an important epigenetic control mechanism in a wide array of eukaryotic organisms and generally carried out by proteins of the C-5 DNA methyltransferase family (DNMTs). (mdpi.com)
  • We have classified these motifs into three types according to their sequence similarity and have found that they are prevalent in many eukaryotic nuclear proteins in single or multiple copies. (embl.de)
  • He further utilized the model organism, Schizosaccharomyces pombe , to investigate the role of the SPN/FACT complex in eukaryotic DNA replication. (usc.edu)
  • We identify highly conserved genes important for eukaryotic cell organization including those required for the cytoskeleton, compartmentation, cell-cycle control, proteolysis, protein phosphorylation and RNA splicing. (inrae.fr)
  • The origin of the eukaryotic N- glycosylation pathway is not unique and less straightforward than previously thought: some basic components likely have proteoarchaeal origins, but the pathway was extensively developed before the eukaryotic diversification through multiple gene duplications, protein co-options, neofunctionalizations and even possible horizontal gene transfers from bacteria. (biomedcentral.com)
  • More than half of all eukaryotic proteins are glycoproteins, and 90 % of those are N- glycosylated [ 33 ]. (biomedcentral.com)
  • Sequencing of the A. oryzae genome was accomplished using the whole-genome shotgun (WGS) approach. (nature.com)
  • Comparative analysis of the sequence of chromosome 20 to whole-genome shotgun-sequence data of two other vertebrates, the mouse Mus musculus and the puffer fish Tetraodon nigroviridis, provides an independent measure of the efficiency of gene annotation, and indicates that this analysis may account for more than 95% of all coding exons and almost all genes. (inrae.fr)
  • Sequence data is accepted on the basis of individual sanger sequences as well as whole genome level. (candidagenome.org)
  • We performed whole-genome (WG) de novo sequencing and complete assembly of S. fibuligera KJJ81 and KPH12, two isolates from wheat-based Nuruk in Korea. (biomedcentral.com)
  • Amino-acid sequence and structure comparison suggested that the enzyme belongs to a group of enzymes classified as archaeal Holliday junction-resolving enzymes, which are typically divalent metal ion-binding dimers that are able to cleave X-shaped dsDNA-Holliday junctions (Hjs). (iucr.org)
  • The 37-Mb genome was predicted to contain a total of 12,074 genes encoding proteins with a length greater than 100 amino acid residues (see Methods). (nature.com)
  • The hallmark of the family is a domain of approximately 260 amino acids that has a high degree of sequence similarity in all sirtuins. (biomedcentral.com)
  • Based on their amino acid sequences, hydropathy profiles and solvent solubility, two classes of hydrophobins are distinguished. (biomedcentral.com)
  • Drosophila's chromosome ends are capped by arrays of retrotransposons and the adjacent subtelomeric DNA consists of repetitive elements called telomere-associated sequences (TAS) [ 4 ]. (biomedcentral.com)
  • Lactose regulatory protein LAC9 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / N. (yeastrc.org)
  • The C. albicans strain SC5314 genome sequence and diploid contig-level assembly by the Stanford Genome Technology Center was published in Jones et al. (candidagenome.org)
  • Home page for C. albicans strain 1161 sequencing at the Sanger Institute . (candidagenome.org)
  • Multi Locus Sequence Typing (MLST) schemes and isolate databases for C. albicans , C. glabrata , C. krusei , and C. tropicalis are available from PubMLST.org The website offers tools for strain typing and epidemiology, as well as extensive, manually curated isolate databases. (candidagenome.org)
  • We performed 12-generation temperature-shift experiments using the wild-type C. elegans and a mutant strain that lacks the germline-specific nuclear Argonaute protein HRDE-1/WAGO-9. (biomedcentral.com)
  • With the advent of fully sequenced genomes, the computational study of orthologous protein relationships in evolution, comparative genomics, but also for substantiating the evolutionary conservation of fundamental cellular processes increased exponentially. (biomedcentral.com)
  • Protein families are in practice more complicated, as genomes have evolved substantially, leading amongst others to gene duplications and losses [ 2 ]. (biomedcentral.com)
  • BLAST search for sequence similarity within microbial genomes. (candidagenome.org)
  • Comparing the 3.27-megabase (Mb) genome sequence of an armadillo-derived Indian isolate of the leprosy bacillus with that of Mycobacterium tuberculosis (4.41 Mb) provides clear explanations for these properties and reveals an extreme case of reductive evolution. (inrae.fr)
  • Blasting online sequence databases is a way to retrieve orthologs for a protein of interest. (shopambitionhustle.com)
  • Searching the orthologs of a given protein or DNA sequence is one of the most important and most commonly used Bioinformatics methods in Biology. (biomedcentral.com)
  • Programs like BLAST or the orthology search engine Inparanoid can be used to find orthologs when the similarity between two sequences is sufficiently high. (biomedcentral.com)
  • Based on relaxed sequence similarity searches, morFeus selects sequences based on the similarity of their alignments to the query, tests for orthology by iterative reciprocal BLAST searches and calculates a network score for the resulting network of orthologs that is a measure of orthology independent of the E-value. (biomedcentral.com)
  • Detecting remotely conserved orthologs of a protein using morFeus thus requires no manual intervention. (biomedcentral.com)
  • Trying to find the orthologs of a given protein or DNA sequence has co-evolved with sequencing itself. (biomedcentral.com)
  • Manual comparison of sequence alignments is oftentimes used to detect remotely conserved orthologs in the twilight zone. (biomedcentral.com)
  • In turn, fold conservation implies the conservation of a sequence pattern of hydrophilic/hydrophobic and size-restricted residues within the protein family. (beds.ac.uk)
  • Tools for analysis of nucleotide sequence patterns in Assembly 19. (candidagenome.org)
  • Knowledge of the complete genomic DNA sequence of an organism allows a systematic approach to defining its genetic components. (inrae.fr)
  • Developing predictive models of multi-protein genetic systems to understand and optimize their behavior remains a combinatorial challenge, particularly when measurement throughput is limited. (omictools.com)
  • RNAi knockdown of human kinetochore protein CENP-H". Biochemical and Biophysical Research Communications. (wikipedia.org)
  • It was initially discovered as a biochemical pathway in which double-stranded RNA (dsRNA) leads to degradation of target mRNA in a highly sequence-specific manner [ 1 , 2 ]. (biomedcentral.com)
  • The completely sequenced S. fibuligera genome with high-quality annotation and RNA-Seq analysis establishes an important foundation for functional inference of S. fibuligera in the degradation of fermentation mash. (biomedcentral.com)
  • Examples of function annotation using dissectHMMER, including the function discovery of an uncharacterized membrane protein Q9K8K1_BACHD (WP_010899149.1) as a lactose/H+ symporter, are presented. (beds.ac.uk)
  • We now describe the complete re-annotation and re-analysis of the C. jejuni NCTC11168 genome using current database information, novel tools and annotation techniques not used during the original annotation.ResultsRe-annotation was carried out using sequence database searches such as FASTA, along with programs such as TMHMM for additional support. (omictools.com)
  • The C. jejuni NCTC11168 re-annotation reduced the total number of coding sequences from 1654 to 1643, of which 90.0% have additional information regarding the identification of new motifs and/or relevant literature. (omictools.com)
  • The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. (wikipedia.org)
  • 2007 ). Several Oxa1-type insertases in the ER serve as catalytically active core subunits in the ER membrane protein complex (EMC), the guided entry of tail-anchored (GET) and the GET- and EMC-like (GEL) complex. (tcdb.org)
  • Background: This gene encodes one of seven subunits of the human Arp2/3 protein complex. (icbinhibitor.com)
  • They then export some of these proteins as well some mitochondrially-encoded proteins to the inter membrane space, or they insert them into the inner membrane. (tcdb.org)
  • Bacteria also export (to the periplasm) N-tails of membrane proteins synthesized without leader sequences by a Sec (Type IIPS)-independent mechanism. (tcdb.org)
  • Insertion of a membrane protein via the Oxa1p export machinery follows the 'positive-inside' rule for membrane protein topology. (tcdb.org)
  • The matrix exposed C-terminal α-helical domain of Oxa1 can bind mitochondrial ribosomes to facilitate co-translational insertion of proteins into the mitochondrial membrane ( Jia et al . (tcdb.org)
  • 2001 ). Mitochondria have two, one for co-translational, and one for post-translational insertion of membrane proteins. (tcdb.org)
  • 2022 ). The YidC transmembrane (TM) groove is essential for a high- affinity interaction, and the hydrophilic nature of the YidC groove plays an important role in protein transport across the cytoplasmic membrane bilayer to the periplasmic side. (tcdb.org)
  • At different stages of the insertion process, conformational changes in YidC's TM domain and membrane core have a mechanistic effect on Pf3 coat protein insertion. (tcdb.org)
  • The genome of Aspergillus oryzae , a fungus important for the production of traditional fermented foods and beverages in Japan, has been sequenced. (nature.com)
  • functional characterization of nat/ncs2 proteins of aspergillus brasiliensis reveals a genuine xanthine-uric acid transporter and an intrinsically misfolded polypeptide. (liverpool.ac.uk)
  • Pfam is a large collection of protein families, represented by multiple sequence alignments and hidden markov models (hmms). (shopambitionhustle.com)
  • HMG-I(Y)] or as part of a multidomain protein [e.g. (embl.de)
  • Formins are multidomain proteins conserved from plants to fungi and vertebrates. (biomedcentral.com)
  • The blocks of A. oryzae -specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. (nature.com)
  • The similarities between the MHC and other compatibility systems will be emphasized with the view that the MHC is primarily an inbreeding avoidance system and its role in histocompatibility is a secondary one 1 . (tripod.com)
  • While the genomic sequence of DcAOX2a was previously described, we characterize here the complete genomic sequence of DcAOX1 . (frontiersin.org)
  • 2018 ). Through YidC, proteins are inserted into the lipid bilayer via the SecYEG-dependent complex, but YidC functions as a chaperone in protein folding processes. (tcdb.org)
  • It shares mechanistic similarities based on the use of polyisoprenol lipid carriers with other glycosylation pathways involved in the synthesis of bacterial cell wall components (e.g. peptidoglycan and teichoic acids). (biomedcentral.com)
  • A protein discovered in the late 1970s has been shown to defend human cells from becoming cancerous. (shopambitionhustle.com)
  • The finished sequence of human chromosome 20 comprises 59,187,298 base pairs (bp) and represents 99.4% of the euchromatic DNA. (inrae.fr)
  • Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ARPC1A. (icbinhibitor.com)
  • To improve our understanding of this important human pathogen, the C. jejuni NCTC11168 genome was sequenced and published in 2000. (omictools.com)
  • Mitochondria import nuclearly-encoded proteins, made in the cell cytoplasm, into the mitochondrial matrix where their mitochondrial targeting sequences are removed by proteolysis. (tcdb.org)
  • Syntenic analysis of the three aspergilli revealed the presence of syntenic blocks and A. oryzae -specific blocks of sequence (lacking synteny with the two other aspergilli) in a mosaic manner throughout the A. oryzae genome ( Fig. 1 ). (nature.com)
  • Thus, the increase in genome size seems to be due to an A. oryzae lineage-specific acquisition of sequence, rather than loss of sequence in A. nidulans and A. fumigatus . (nature.com)
  • If, on the other hand, A. nidulans and A. fumigatus are assumed to have lost 7-9 Mb of sequence after branching off from their A. oryzae -like ancestor, a greater proportion of syntenic blocks would be conserved between each of them and A. oryzae than between the two. (nature.com)
  • This suggests that the genome size differences are largely due to sequence acquisition in A. oryzae . (nature.com)
  • to facilitate higher yields of proteins of interest, we constructed the pasop vector to express heterologous proteins in a. oryzae. (liverpool.ac.uk)
  • Blast protein - perform protein blast searches using a. (shopambitionhustle.com)
  • Blastp (protein blast): compares one or more protein query sequences to a subject protein sequence or a database of protein sequences. (shopambitionhustle.com)
  • You can find most of the protein databases that were available here at the uniprot blast service. (shopambitionhustle.com)
  • Slutter man brått med kortisontabletter vil kroppen være uten dette livsviktige stoffet og pasienten vil raskt bli svært alvorlig syk, protein blast. (shopambitionhustle.com)
  • Nucleotide to protein blast (blastx). (gpiaca.com)
  • Blast finds region of similarity between biological sequences. (gpiaca.com)
  • Ik vraag me af en hoop dat het volledig stoppen met het gebruik al deze aandoeningen kan stoppen of in elk geval verbeteren, protein blast. (gpiaca.com)
  • Conclusions: Low endogenous levels of testosterone may be related to reduced cognitive ability, and testosterone substitution may improve some aspects of cognitive ability, protein blast. (gpiaca.com)
  • The program compares nucleotide or protein sequences to sequence databases. (gpiaca.com)
  • Characterization of a novel kinetochore protein, CENP-H". The Journal of Biological Chemistry. (wikipedia.org)
  • We then combined the search algorithm with system-level kinetic modeling, requiring the construction and characterization of 73 variants to build a sequence-expression-activity map (SEAMAP) for a biosynthesis pathway. (omictools.com)
  • Blastn - dna query to dna database, blastp - protein query to protein database, blastx - translated (6. (gpiaca.com)
  • pombe replication origin, ars1. (embl.de)
  • pombe origins of replication, which differ significantly from those of Sa. (embl.de)
  • Replication origins in the DNA nucleate the ordered assembly of protein factors to form a prereplication complex (preRC) that is poised for DNA synthesis. (usc.edu)
  • 2004). View the A19 paper and download the A19 supplementary information and sequence files or the original A19 sequence trace files . (candidagenome.org)
  • ClinVar variants which are reported in the ClinVar database and could be investigated for pathogeneity with protein modelling (see supplementary Table S6). (springer.com)
  • Electronic supplementary material Additional file 1: The sequences of PCR primers. (cox-inhibitors.com)
  • Hide enter nucleotide query sequence. (gpiaca.com)
  • Subsequent statistical stratification of the fold-related hits into cohorts of functionally-related domains allows for the function postulation of the query sequence. (beds.ac.uk)
  • AT-hook motifs identified in a wide variety of DNA-binding proteins. (embl.de)
  • Using pattern searches and position-dependent matrices, we have extracted the AT-hook motifs present in a non-redundant protein sequence database. (embl.de)
  • Genome downsizing and the current mosaic arrangement appear to have resulted from extensive recombination events between dispersed repetitive sequences. (inrae.fr)