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  • nucleic
  • Accordingly, the invention relates to the preparation of this enzyme from various sources by recombinant techniques, to nucleic acid segments which encode the enzyme or which can be used as probes for the selection of related sequences, as well as to assay methods for the identification of candidate substances which will affect the activity of the enzyme. (google.co.uk)
  • Proteins use their functional regions to exploit various activities, including binding to other proteins, nucleic acids, or drugs. (springer.com)
  • Superfamily
  • The Major Facilitator Superfamily (MFS), the largest family of secondary transport proteins, catalyzes transport of a wide range of substrates. (pnas.org)
  • This information is exceptionally difficult to obtain with the Major Facilitator Superfamily (MFS) because of the broad sequence diversity of the members. (pnas.org)
  • The exceptionally diverse Major Facilitator Superfamily (MFS) includes over 10,000 sequenced members and is comprised of 74 families with members from Archaea to Homo sapiens ( 1 ⇓ - 3 ). (pnas.org)
  • Probst WC, Snyder LA, Schuster DI, Brosius J, Sealfon SC (1992) Sequence alignment of the G protein-coupled receptor superfamily. (springer.com)
  • functional
  • However, with respect to sequence information, many functionally and structurally important sites are hard to distinguish and consequently a large number of incorrectly predicted functional sites have to be expected. (uni-regensburg.de)
  • To determine which residues play an obligatory role in the mechanism and to create a library of mutants with a single-Cys residue at each position of the molecule for structure/function studies, each residue was replaced individually with Cys in a functional mutant devoid of native Cys residues ( 7 ). (pnas.org)
  • V194G and R237L, which elicit functional alterations leading to disruption of ligand binding and G protein coupling, respectively. (springer.com)
  • Functional sites of the proteins have a tendency to be more conserved than the rest of the protein surface. (springer.com)
  • Tuncbag N, Gursoy A, Guney E et al (2008) Architectures and functional coverage of protein-protein interfaces. (springer.com)
  • clusters
  • Additionally, we find evidence that extensive gene conversion contributes to the maintenance of sequence identity within chromosome-level clusters of MSP genes. (g3journal.org)
  • structurally
  • Keskin O, Tsai C-J, Wolfson H, Nussinov R (2004) A new, structurally nonredundant, diverse data set of protein-protein interfaces and its implications. (springer.com)
  • yet cross-species comparison of PTMs is difficult since they often lie in structurally disordered protein domains. (garvan.org.au)
  • Kinesin-1 and mitochondrial motility control by discrimination of structurally equivalent but distinct subdomains in Ran-GTP-binding domains of Ran-binding protein 2. (nih.gov)
  • conformational
  • We constructed intra-protein network graphs to discriminate the global conformational changes induced by mutations. (springer.com)
  • Frontispiece : probing transient conformational States of proteins by solid‐state r1ρ relaxation‐dispersion NMR spectroscopy. (ibs.fr)
  • genes
  • This protein forms a heterodimeric complex and binds and activates, in a glucose-dependent manner, carbohydrate response element (ChoRE) motifs in the promoters of triglyceride synthesis genes. (genetex.com)
  • Identifying
  • Recent technical advances have led to several high-throughput experimental methods for identifying partners in protein-RNA complexes, but determining RNA-binding residues in proteins is still expensive and time-consuming. (iastate.edu)
  • This chapter focuses on available computational methods for identifying which amino acids in an RNA-binding protein participate directly in contacting RNA. (iastate.edu)
  • Mutations
  • Additionally, these mutations lead to compact ICL3 region compared to the wild type, indicating that the receptor would be inaccessible for G protein coupling. (springer.com)
  • If two sequences in an alignment share a common ancestor, mismatches can be interpreted as point mutations and gaps as indels (that is, insertion or deletion mutations) introduced in one or both lineages in the time since they diverged from one another. (wikipedia.org)
  • Furthermore, these mutations fall preferably onto loci in sequence neighborhoods that are high scoring in terms of germline fitness. (genetics.org)
  • intermediate
  • This has prevented us from answering fundamental structure-function relationship questions related to the biomechanical role of intermediate filaments, which is crucial to link structure and function in the protein material's biological context. (asme.org)
  • PTMs
  • BACKGROUND: Most biological processes are influenced by protein post-translational modifications (PTMs). (garvan.org.au)
  • selective
  • Measuring hydrogen exchange in proteins by selective water saturation in 1H‐ 15N SOFAST/BEST‐type experiments : advantages and limitations. (ibs.fr)
  • Computational
  • Li Y, Hou T, Goddard I (2010) Computational modeling of structure-function of G protein-coupled receptors with applications for drug design. (springer.com)
  • A variety of computational algorithms have been applied to the sequence alignment problem. (wikipedia.org)
  • methods
  • The DNA sequence encoding steroid 5α-reductase 2, the major active isozyme of human genital tissue, is disclosed herein, in addition to methods and compositions for its preparation and pharmacological analysis. (google.co.uk)
  • de Juan D, Pazos F, Valencia A (2013) Emerging methods in protein co-evolution. (springer.com)
  • receptor
  • Several dementia-specific citrulline residues were also identified in soluble proteins previously categorized as pro-immunogenic, which include the receptor P2X7, alpha-internexin, GFAP, CNP, MBP, and histones. (biomedcentral.com)
  • recognition
  • Discola KF, Forster A, Boulay F, Simorre JP, Attree I, Dessen A, and Job V Membrane and chaperone recognition by the major translocator protein PopB of the type III secretion system of Pseudomonas aeruginosa. (ibs.fr)
  • Membrane and chaperone recognition by the major translocator protein PopB of the Type III secretion system of Pseudomonas aeruginosa. (ibs.fr)