Sequence protein domain carrier proteins. Medical search. Frequent questions

One PCR product was sequenced and appeared to be in part homologous to the pla gene, but the adjacent sequence displayed high homology to genes coding for replicon ( rep) proteins in several bacterial genera in the family Enterobacteriaceae , e.g. (cdc.gov)
This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members. (phosphosolutions.com)
Previously undetected bacterial homologues were identified for# plant pathogenesis-related proteins, Pad1, von Willebrand factor type A, src homology 3 and YWTD repeat-containing domains. (embl.de)

Our findings suggest that macro domain tags can be employed in recombinant protein expression in E. coli, and in carrier-driven crystallization. (rcsb.org)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
2 μg/lane of Biotinylated Recombinant Human IFN‑ gamma R1/CD119 Avi-tag His-tag Protein (Catalog # AVI11030) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42‑55 kDa. (rndsystems.com)
The recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. (prosci-inc.com)
When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. (prosci-inc.com)
Recombinant Human HB-EGF is a 9.7 kDa protein containing 86 amino acid residues, corresponding to the extracellular EGF-like and heparin-binding domains of the full length HB-EGF protein. (prosci-inc.com)
The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. (justia.com)
The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions. (justia.com)
The preparation of valuable recombinant (genetically engineered) polypeptides, for example pharmaceutical proteins, relies frequently on techniques which involve the production of these polypeptides as fusion or hybrid proteins. (justia.com)
4083. The ED 50 for this effect is 1.5-6 µg/mL in the presence of 10 ng/mL of Recombinant Human NRG1‑ beta 1/HRG1‑ beta 1 Extracellular Domain (Catalog # 377-HB ). (bio-techne.com)

Overview of all the structural information available in the PDB for UniProt: Q6NUK1 (Human Calcium-binding mitochondrial carrier protein SCaMC-1) at the PDBe-KB. (wikipedia.org)

The sequence identity among all species is about 30%, and their structures and functions should be similar in all microbes. (biorxiv.org)

The STAS domain forms a complex with the acyl carrier protein, ACP and malonyl-ACP, and the complex has been determined by x-ray crystallography (PDB# 3NY7). (lbl.gov)

Pla genes obtained from 2 of the peritoneum samples collected from black rats were sequenced and appeared to be identical (GenBank accession no. (cdc.gov)

The structures exhibit the same general fold as the majority of known carrier protein structures, in spite of the complex biosynthetic role that PCP domains from the final non-ribosomal peptide synthetase module must play in glycopeptide antibiotic biosynthesis. (rcsb.org)
Antibiotic biosynthesis monooxygenase domain [InterProScan]. (ntu.edu.sg)
We examined the enzyme protein and biosynthesis of human trifunctional protein harboring enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and 3-ketoacyl-CoA thiolase activity in cultured skin fibroblasts from two patients with long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency. (rhea-db.org)
Heat shock proteins (HSP) are molecular chaperones which facilitate the biosynthesis and maturation of proteins within cells (protein folding). (surfinglion.com)
Built YchM deletion exhibited artificial lethality with multiple fatty acidity biosynthesis genes, as well as the YchM polypeptide bodily interacted numerous proteins from the fatty acidity biosynthetic pathway. (exposed-skin-care.net)
Certainly, STAS domain-encoding SulP genes have already been found as the different parts of antibiotic operons, like the BLM bleomycin biosynthesis operon of (Du et al. (exposed-skin-care.net)

The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. (phosphosolutions.com)
protein_coding" "AGT23894","N559_2182","Klebsiella pneumoniae","putative ABC-type taurine transport system periplasmic binding component [Ensembl]. (ntu.edu.sg)
protein_coding" "AGT24100","N559_2404","Klebsiella pneumoniae","putative ABC transporter substrate binding protein [Ensembl]. (ntu.edu.sg)
protein_coding" "AGT25156","N559_3508","Klebsiella pneumoniae","putative short-chain dehydrogenase/reductase SDR [Ensembl]. (ntu.edu.sg)
RFC proteins possess 12 putative transmembrane a-helical spanners (TMSs) and evidence for a 12 TMS topology has been published for the human RFC. (nih.gov)
2010). NMR answer structures have already been resolved for the STAS BIIE 0246 supplier domain name of putative sulfate transporter Rv1739c from (Sharma et al. (exposed-skin-care.net)
Open up in another window Physique 2 Structural topology style of SLC26 polypeptides displaying the brief cytoplasmic N-terminal area accompanied by a transmembrane domain name with 12 putative membrane-spanning -helices, as well as the C-terminal cytoplasmic area, largely composed of the STAS domain name. (exposed-skin-care.net)

transmembrane domain, AhpC/TSA family [InterProScan]. (ntu.edu.sg)
Mature human IFNGR1 consists of an extracellular domain (ECD) with 2 Ig-like domains, a transmembrane domain and an intracellular domain with both Jak1 and Stat1 binding motifs. (rndsystems.com)

Affibody molecules hold great promise as carriers of cytotoxic drugs for cancer therapy due to their typically high affinity, easy production, and inherent control of the drug molecules' loading and spatial arrangement. (mdpi.com)
Usually, the NOE and NMRD in B-DNA is expected to be less ``integral'' than methods can only provide bounds on the residence water molecules buried inside proteins. (lu.se)

Calcium-binding mitochondrial carrier protein SCaMC-1 is a protein that in humans is encoded by the SLC25A24 gene. (wikipedia.org)
In the majority of cases, Rf genes produce proteins that act directly on the CMS conferring mitochondrial transcripts by binding them specifically and promoting processing events. (frontiersin.org)
The male sterilizing factors are produced by recombination of the mitochondrial genome and consist of unusual genes or open reading frames (ORFs) that usually contain a portion of functional mitochondrial genes and sequences of unknown origin ( Hanson and Bentolila, 2004 ). (frontiersin.org)
These unusual ORFs are maternally inherited, and effectively translated into novel mitochondrial proteins, with the resulting failure to produce functional pollen as the sole observed phenotype ( Chen and Liu, 2014 ). (frontiersin.org)
In the majority of cases, Rf genes produce proteins that bind specifically to the CMS conferring transcripts in the mitochondria and promote processing events leading to a strong reduction in the production of mitochondrial CMS-inducing proteins (reviewed in Chen and Liu, 2014 ). (frontiersin.org)
Mitochondrial trifunctional protein deficiency. (rhea-db.org)

protein_coding" "AAC74185","ptsG","Escherichia coli","fused glucose-specific PTS enzymes: IIB component/IIC component [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74323","adhE","Escherichia coli","fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74850","yeaD","Escherichia coli","D-hexose-6-phosphate epimerase-like protein [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74888","manY","Escherichia coli","mannose-specific enzyme IIC component of PTS [Ensembl]. (ntu.edu.sg)

Search proteins in UniProtKB for this molecule. (rhea-db.org)

Obtaining well-ordered crystals remains a significant challenge in protein X-ray crystallography. (rcsb.org)

Click molecule labels to explore molecular sequence information. (nih.gov)
The structures were solved by molecular replacement, using isolated macro domain structures as search models. (rcsb.org)
The antibody detects a 220 kDa* protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of adult mouse brain and Cos-7 cells transfected with mouse Plexin-A1. (phosphosolutions.com)
With more than 500 SH3 domains in the human genome, binding selectivity is a key issue in understanding the molecular basis of SH3 domain interactions. (ox.ac.uk)

In von Willebrand factor, the type A domain (vWF) is the prototype for a protein superfamily. (embl.de)
TIM barrel domain, Glyoxalase/fosfomycin resistance/dioxygenase domain, Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily [InterProScan]. (ntu.edu.sg)

Human GITR Ligand consists of a 50 amino acid cytoplasmic domain, a 21 aa transsmembrane segment, and a 128 aa extracellular domain (ECD). (genscript.com)

The diminution of trifunctional protein was associated with a decreased activity of enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and 3-ketoacyl-CoA thiolase, when measured using medium-chain to long-chain substrates. (rhea-db.org)

hence the protein may regulate transcription by competitively binding to specific DNA-binding transcription factors. (prospecbio.com)
Tet proteins regulate gene expression by removing methyl groups from DNA bases. (natureasia.com)
Two of these differ in the presence of juxtamembrane extracellular sequences which regulate the ability of TACE (TNF-alpha converting enzyme) to proteolytically cleave ErbB4 from the cell surface. (bio-techne.com)

Mutations of NPHS1 or NPHS2, the genes encoding for the glomerular podocyte proteins nephrin and podocin, cause steroid-resistant proteinuria. (nih.gov)

The ECD of human IFNGR1 shares 50% amino acid sequence identity with mouse IFNGR1. (rndsystems.com)
Mature mouse IL-22BP consists of two tandem Fibronectin type-III domains and shares 85% and 68% amino acid sequence identity with rat IL-22BP and the short isoform of human IL-22BP, respectively (6, 14). (rndsystems.com)

In addition, mice lacking NEPH1 develop a nephrotic syndrome that resembles NPHS mutations, suggesting that all three proteins are essential for the integrity of glomerular podocytes. (nih.gov)

This protein family is largely expanded in land plant genomes. (frontiersin.org)

We have structurally characterized the final peptidyl carrier protein domain of the teicoplanin non-ribosomal peptide synthetase machinery: this domain is believed to mediate the interactions with tailoring Oxy enzymes in addition to its function as a shuttle for intermediates between multiple non-ribosomal peptide synthetase domains. (rcsb.org)
VWA domains in extracellular eukaryotic proteins mediate adhesion via metal ion-dependent adhesion sites (MIDAS). (embl.de)
The Reduced Folate Carrier (RFC) Family (TC 2.A.48) Members of the RFC family mediate the uptake of folate, reduce folate, derivatives of reduced folate and the drug, methotrexate. (nih.gov)

LMO1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol. (prospecbio.com)
SRA1 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) , 0.1MNaCl ,10% glycerol and 1mM DTT. (prospecbio.com)

The enzyme transfers CO 2 from glutaconyl-CoA to a biotin carrier protein (the γ-subunit) that is subsequently decarboxylated by the carboxybiotin decarboxylation site within the Na + pumping beta subunit (Gcdβ). (tcdb.org)

The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. (phosphosolutions.com)

Dynamics of p38 MAP kinase inferred from a structural ensemble using PCA is compared to intrinsic dynamics of the protein modeled using ANM. (bahargroup.org)
Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. (bio-techne.com)
Anaplastic lymphoma kinase (ALK) protein may be detected in most cases (60-70%) of systemic ALCL by immunohistochemistry. (medscape.com)
In the poster section, Joanna Shisler (University of Illinois at Urbana-Champaign [UIUC], Urbana) reported that the modified virus, Ankara, activates nuclear factor κB through the mitogen-activated protein kinase, extracellular signal–regulated kinase (MEK)/extracellular signal–regulated kinase (ERK) pathway, possibly facilitating the host immune response. (cdc.gov)

The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)

A major difference was found between the distributions of enzyme families that are typically found in all three divisions of cellular life and non-enzymatic domain families that are usually eukaryote-specific. (embl.de)
GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams ). (lbl.gov)
Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
The cleavage reaction is performed by contacting the fusion protein with a proteolytic enzyme under appropriate conditions. (justia.com)

The domain adopts a classic alpha/beta Rossmann fold and contains an unusual metal ion coordination site at its surface. (embl.de)

LMO1 encodes a transcriptional regulator which contains two cysteine-rich LIM domains but lacks a DNA-binding domain. (prospecbio.com)
This gene encodes a carrier protein that transports ATP-Mg exchanging it for phosphate. (wikipedia.org)
The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF. (prosci-inc.com)

The α-subunits of the oxaloacetate and methylmalonyl-CoA decarboxylases are homologous to many biotin-containing enzymes including (1) pyruvate carboxylases, (2) homocitrate synthases, (3) biotin carboxyl carrier proteins, (4) isopropylmalate synthases and (5) acyl-CoA carboxylase. (tcdb.org)

protein_coding" "AGT24181","N559_2495","Klebsiella pneumoniae","cytochrome C biogenesis protein transmembrane region [Ensembl]. (ntu.edu.sg)

For both mechanisms a protein carrier/trafficker was required, since galectin-9 lacks a secretion sequence. (bmj.com)
The crystal structure of a complex between the C-terminal SH3 domain (SH3C) of Mona/Gads and a SLP-76 peptide has now been solved to 1.7 A. The peptide lacks the canonical SH3 domain binding motif P-x-x-P and does not form a frequently observed poly-proline type II helix. (ox.ac.uk)

Using solution state NMR, we have determined structures of this PCP domain in two states, the apo and the post-translationally modified holo state, both of which conform to a four-helix bundle assembly. (rcsb.org)
These structures thus support the hypothesis that it is subtle rearrangements, rather than dramatic conformational changes, which govern carrier protein interactions and selectivity during non-ribosomal peptide synthesis. (rcsb.org)
Outward-facing (OF) and inward-facing (IF) structures of Glt Ph show a large displacement of the core domains. (bahargroup.org)
This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)

All of these procedures result in the production a hybrid protein in which the protein of interest is linked to an additional polypeptide. (justia.com)

In addition, SRA1 mediates transcriptional coactivation of steroid receptors ligand-dependently via the steroid-binding domain (AF-2). (prospecbio.com)
NAD(P)-binding domain [InterProScan]. (ntu.edu.sg)
NAD binding domain [InterProScan]. (ntu.edu.sg)
2Fe-2S iron-sulfur cluster binding domain, 2Fe-2S ferredoxin-type iron-sulfur binding domain [InterProScan]. (ntu.edu.sg)
The RNA-binding protein FMR1 has a key role in the neurodevelopmental disorder fragile X syndrome, but the RNAs targeted by the protein were mostly unknown. (natureasia.com)
Coevolution of NEF-binding residues analzyed using mutual information is displayed for the Hsp70 ATPase domain. (bahargroup.org)
Probable molybdopterin binding domain [Interproscan]. (ntu.edu.sg)
Molydopterin dinucleotide binding domain, Molybdopterin oxidoreductase Fe4S4 domain [Interproscan]. (ntu.edu.sg)
4Fe-4S binding domain, 4Fe-4S dicluster domain [Interproscan]. (ntu.edu.sg)
Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). (rndsystems.com)
Fusion partners known to the prior art include maltose binding protein (Di Guan et al. (justia.com)
Structural basis for SH3 domain-mediated high-affinity binding between Mona/Gads and SLP-76. (ox.ac.uk)
Most of the circulating testosterone is bound to carrier proteins (SHBG = sex hormone-binding globulin). (cdc.gov)
He also showed that double-stranded (ds)RNA- and ZDNA binding proteins had a role in poxvirus pathogenesis. (cdc.gov)

Secondly, those proteins which are present in soluble form in the cytoplasm often are subject to degradation by host specific enzymes, thus reducing the amounts of active protein that can be recovered. (justia.com)
Enzymes known to the prior art as "proteolytic enzymes" have been found to be particularly well suited for the cleavage of fusion proteins. (justia.com)
SH3 domains are protein recognition modules within many adaptors and enzymes. (ox.ac.uk)

PPR proteins represent the most frequent protein class among identified Rfs and they exhibit ideal characteristics to evolve into restorer of fertility when the mechanism of restoration implies a post-transcriptional action. (frontiersin.org)

Hits to curated proteins without experimental data as to their function are never considered high confidence. (lbl.gov)
This model correctly accounts for the experimental data over a large scattering domain. (surfinglion.com)

N-terminal domain, C-terminal domain, FGGY family of carbohydrate kinases [InterProScan]. (ntu.edu.sg)
Aldehyde dehydrogenase domain [InterProScan]. (ntu.edu.sg)
NmrA-like domain [InterProScan]. (ntu.edu.sg)
EcpB C-terminal domain [InterProScan]. (ntu.edu.sg)
Protein of unknown function (DUF520) [Interproscan]. (ntu.edu.sg)
tRNA synthetase class II core domain (G, Seryl-tRNA synthetase N-terminal domain [Interproscan]. (ntu.edu.sg)
MGS-like domain [Interproscan]. (ntu.edu.sg)
Peptidase family M20/M25/M40, Peptidase dimerisation domain [Interproscan]. (ntu.edu.sg)
AAA domain [Interproscan]. (ntu.edu.sg)

Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)

2010). The mammalian and bacterial STAS domains reported to day have already been monomeric in answer. (exposed-skin-care.net)
A small amount of bacterial SulP transporters absence a C-terminal STAS domain name, but have in its place an enzymatically energetic (Nishimori et al. (exposed-skin-care.net)

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. (lbl.gov)

Eukaryotic signalling domain homologues in archaea and bacteria. (embl.de)
Phyletic distributions of eukaryotic signalling domains were studied using recently developed sensitive methods for protein sequence analysis, with an emphasis on the detection and accurate enumeration of homologues in bacteria and archaea. (embl.de)
the only other organisms known to live in environments this extreme are members of the domain Archaea . (eol.org)

The PYRIN domain-only protein POP3 inhibits ALR inflammasomes and regulates responses to infection with DNA viruses. (ox.ac.uk)
Background Galectin-9 is a member of the family of lectin proteins and crucially regulates human immune responses, particularly because of its ability to suppress the anticancer activities of T lymphocytes and natural killer cells. (bmj.com)

PPR proteins have in common a canonical P-type 35 amino acid domain repeated in tandem up to 30 times. (frontiersin.org)

It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. (bio-techne.com)

The existence of a concatenated pla-rep sequence in rat tissue samples was confirmed by amplification of a PCR product from a primer targeting the pla gene, combined with a primer targeting the rep gene sequence that was acquired by using the genewalking procedure. (cdc.gov)
Signs of cytoprotection through up-regulation of so-called heat shock proteins, indications of tissue alterations by proteases (stromelysin) and finally. (surfinglion.com)

Anatomy8

Organisms14

Chemicals and Drugs106

Analytical, Diagnostic and Therapeutic Techniques and Equipment41

Phenomena and Processes78

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science13

Health Care2

Homology3

Recombinant12

Uniprot1

Species1

Acyl carrier1

Accession1

Biosynthesis6

Putative7

Transmembrane domain2

Molecules2

Mitochondrial6

Escherichia4

UniProtKB1

Crystallography1

Molecular4

Superfamily2

Extracellular domain1

Enoyl1

Regulate3

Genes1

Amino acid sequence ide2

Mice1

Genomes1

Mediate3

20mM Tris-HCl buffer2

Subunit1

Motifs1

Kinase4

Transporter1

Enzyme4

Fold1

Encodes3

Homologous1

Ensembl1

Lacks2

Structures4

Polypeptide1

Binding14

Enzymes3

Transcriptional1

Experimental Data2

InterProScan9

Biological1

Bacterial2

Relies1

Archaea3

Regulates2

Canonical1

Cytoplasmic domain1

Tissue2