Loading...
  • Genes
  • To study the prevalence and clinical impact of PRC2 aberrations in an unselected childhood ALL cohort (n = 152), we performed PRC2 mutational screenings by Sanger sequencing and promoter methylation analyses by quantitative pyrosequencing for the three PRC2 core component genes EZH2, suppressor of zeste 12 (SUZ12), and embryonic ectoderm development (EED). (cancerindex.org)
  • Targeted deep next-generation sequencing of 30 frequently mutated genes in leukemia was performed to search for cooperating mutations in patients harboring PRC2 aberrations. (cancerindex.org)
  • Such genes significantly affected the metabolism of lipid, cellular ketone, organic acid, and carboxylic acids. (nih.gov)
  • Significant sequence similarity and shared functional domains indicate that these two genes are orthologous genes, inherited from the shared ancestor. (wikipedia.org)
  • citation needed] Given their tremendous importance for biology and bioinformatics, orthologous genes have been organized in several specialized databases that provide tools to identify and analyze orthologous gene sequences. (wikipedia.org)
  • Mutation
  • CS-BLAST (Context-Specific BLAST) is a tool that searches a protein sequence that extends BLAST (Basic Local Alignment Search Tool), (Basic Local Alignment Search Tool) using context-specific mutation probabilities. (wikipedia.org)
  • amino acids
  • 1985) determined that human plasma beta-glycoprotein hemopexin consists of a single polypeptide chain of 439 amino acids residues with six intrachain disulfide bridges and has a molecular mass of approximately 63 kD. (wikipedia.org)
  • Similarities between amino acids or nucleotides are quantified in these substitution matrices. (wikipedia.org)
  • Histogram bar heights represent the fraction of amino acids in profile columns" . (wikipedia.org)
  • The extent of the inaccuracy increases with the number of amino acids in the loop. (wikipedia.org)
  • The loop amino acids' side chains dihedral angles are often approximated from a rotamer library, but can worsen the inaccuracy of side chain packing in the overall model. (wikipedia.org)
  • In general, the most accurate predictions are for loops of fewer than 8 amino acids. (wikipedia.org)
  • Even with all these methods non-template based approaches are most accurate up to 12 residues (amino acids within the loop). (wikipedia.org)
  • nucleotide
  • We then constructed a phylogenetic tree for these birds by the use of nucleotide sequences, choosing the Japanese quail (Coturnix coturnix japonica) as an outgroup. (biomedsearch.com)
  • Direct sequencing of the M-MSP products from the methylated primary samples showed the expected nucleotide changes after bisulphite treatment, therefore confirming complete bisulphite conversion and specificity of MSP (Fig. 2A). (nih.gov)
  • Motifs
  • Within protein domains, shorter signatures known as 'motifs' are associated with particular functions, and motif databases such as PROSITE ('database of protein domains, families and functional sites') can be searched using a query sequence. (wikipedia.org)
  • 1988
  • 1988) concluded that the gene evolved through intron-mediated duplications of a primordial sequence to a 5-exon cluster. (wikipedia.org)
  • residues
  • A) Sequence analysis of the M-MSP product from bisulphite-treated DNA showed that the cytosine [C] residues of CpG dinucleotides were methylated and remained unchanged, whereas all the other C residues were unmethylated and were converted to thymidine [T], indicating complete bisulphite conversion and specificity of MSP. (nih.gov)
  • polymorphism
  • In the present study, we use intraspecific sequence polymorphism of three sex-linked markers to seek evidence for possible events of XY recombination during this species' late Pleistocene history. (nih.gov)
  • quantify
  • Due to the rapid development of "-omics" technology, such as DNA microarrays [ 1 ] and next-generation sequencing (NGS) [ 2 , 3 ], scientists are now able to quantify large numbers of transcripts from organisms simultaneously. (biomedcentral.com)
  • restriction
  • DNA sequencing was successful in identifying a unique BluI restriction site present only in each copy of the 10q26-derived repeat units and a XapI site in all 4q35-derived repeats. (barnardhealth.us)
  • Regions
  • The high degree of homology between the 4q35 and the 10q26 D4F104S1 region is thought to have been responsible for interchromosomal exchanges between these two regions. (barnardhealth.us)
  • With the aim of elucidating in greater detail the genealogical origin of the present domestic fowls of the world, we have determined mtDNA sequences of the D-loop regions for a total of 21 birds, of which 12 samples belong to red junglefowl (Gallus gallus) comprising three subspecies (six Gallus gallus gallus, three Gallus gallus spadiceus, and three Gallus gallus bankiva) and nine represent diverse domestic breeds (Gallus gallus domesticus). (biomedsearch.com)
  • detection
  • This is calculated with the fraction: (pairs correctly aligned)/(pairs aligned) The graph is the benchmark Biegert and Söding used to evaluate homology detection. (wikipedia.org)
  • 1989
  • 1989). "Regional assignment of the loci for adenylate kinase to 9q32 and for alpha 1-acid glycoprotein to 9q31-q32. (wikipedia.org)
  • conservation
  • This is not to be confused with conservation in amino acid sequences, where the amino acid at a specific position has been substituted with a different one that has functionally equivalent physicochemical properties. (wikipedia.org)
  • Conservation of ebv-sisRNA sequence and secondary structure between EBV and other herpesviruses suggest shared functions in latent infection. (wikipedia.org)
  • Domains
  • The development of protein domain databases such as Pfam (Protein Families Database) allow us to find known domains within a query sequence, providing evidence for likely functions. (wikipedia.org)
  • divergence
  • Conversely, an organism that is further removed evolutionarily from another organism is likely to display a greater divergence in the sequence of the orthologs being studied. (wikipedia.org)
  • complete
  • For a complete list see: tclinkdb.txt T-Coffee comes along with a sophisticated sequence reformatting utility named seq_reformat. (wikipedia.org)
  • method
  • Andrej Sali's homology modeling suite MODELLER includes a facility explicitly designed for loop modeling by a satisfaction of spatial restraints method. (wikipedia.org)
  • amino acid residues
  • and wherein the Targeting Moiety is located between the protease cleavage site and the translocation domain, and wherein the protease or fragment thereof and the translocation domain are distanced apart from one another by a maximum of 100 amino acid residues. (patentsencyclopedia.com)
  • 2. The fusion protein according to claim 1, wherein the Targeting Moiety and the protease cleavage site are separated by at most 10 amino acid residues, preferably by at most 5 amino acid residues, and more preferably by at most zero amino acid residues. (patentsencyclopedia.com)
  • 6. The fusion protein according to claim 1, wherein the Targeting Moiety comprises at most 50 amino acid residues, preferably at most 40 amino acid residues, more preferably at least 30 amino acid residues, and most preferably at most 20 amino acid residues. (patentsencyclopedia.com)
  • 93% Negroid The M and N antigens differ at two amino acid residues: the M allele has serine at position 1 (C at nucleotide 2) and glycine at position 5 (G at nucleotide 14) while the N allele has leucine at position 1 (T at nucleotide 2) and glutamate at position 5 (A at nucleotide 14). (wikipedia.org)
  • There are three differences in the first three amino acid residues: the usual form has Tryptophan1-Serine-Threonine-Serine-Glycine5 while Henshaw has Leucine1-Serine-Threonine-Threonine-Glutamate5. (wikipedia.org)
  • motifs
  • Additionally, FUS/TLS has been found to bind a relatively long region in the 3′ untranslated region (UTR) of the actin-stabilising protein Nd1-L mRNA, suggesting that rather than recognising specific short sequences, FUS/TLS interacts with multiple RNA-binding motifs or recognises secondary conformations. (wikipedia.org)
  • intron
  • The double stranded regions of RNA are formed by base-pairing between residues in the close to region of the editing site with residues usually in a neighboring intron but can be an exonic sequence. (wikipedia.org)
  • binds
  • C) DNA-binding domain (DBD): Highly conserved domain containing two zinc fingers that binds to specific sequences of DNA called hormone response elements (HRE). (wikipedia.org)
  • prediction
  • To deal with the situation that many protein sequences have no solved structures, some function prediction servers such as RaptorX are also developed that can first predict the 3D model of a sequence and then use structure-based method to predict functions based upon the predicted 3D model. (wikipedia.org)
  • loci
  • this technique is able to establish point mutations, gene deletions, and short sequence insertions in several genomic loci with increased efficiency and time sensitivity. (wikipedia.org)
  • specific
  • General formula: R CH(NH 2 )COOH, with the side group (R) specific for each individual amino acid. (tolweb.org)
  • We show that the imprint corresponds to a single‐strand DNA break (SSB), which is site‐ but not sequence‐specific. (embopress.org)
  • A specific sequence of nucleotides in DNA is transcribed into mRNA , which is read by the ribosome in a process called translation . (worldheritage.org)
  • Prostatic acid phosphatase (PAP), also prostatic specific acid phosphatase (PSAP), is an enzyme produced by the prostate. (wikipedia.org)
  • cellular
  • The development of context-based and structure based methods have expanded what information can be predicted, and a combination of methods can now be used to get a picture of complete cellular pathways based on sequence data. (wikipedia.org)
  • mutation
  • Mi-I (Mia), Mi-II(Vw), Mi-VII and Mi-VIII are carried on glycophorin A. Mi-I is due to a mutation at amino acid 28 (threonine to methionine: C→T at nucleotide 83) resulting in a loss of the glycosylation at the asparagine26 residue. (wikipedia.org)
  • Mi-II is due to a mutation at amino acid 28 (threonine to lysine:C->A at nucleotide 83). (wikipedia.org)
  • position
  • For more information about the meaning and exact position of a sequence modification, move the cursor over the icon. (rcsb.org)
  • Here the glutamine (Q_) is codon is converted to an arginine (R). The third K/R editing site within the coding sequence is found at amino acid position 15 of the final protein where a Lysine is converted to an Arginine. (wikipedia.org)
  • found
  • The highest levels of acid phosphatase are found in metastasized prostate cancer. (wikipedia.org)
  • Of these, 11 are found within the intronic sequence (1-11), 3 are in the 5'UTR region (5a,5b,5c) while 3 are found within the coding sequence(Y/C site, Q/R site, K/R site). (wikipedia.org)
  • The Q/R site is a second coding region found at amino acid 5 of the final protein. (wikipedia.org)
  • Beyond nucleic acid binding, FUS/TLS was also found to associate with both general and more specialized protein factors to influence the initiation of transcription. (wikipedia.org)
  • region
  • The region that base pairs with the editing region is known as an Editing Complementary Sequence (ECS). (wikipedia.org)
  • It is likely since all the editing sites fall within the duplex region that editing occurs in exonic and intronic sequences at the same time. (wikipedia.org)
  • either
  • The first 39 amino acids of the Dantu antigen are derived from glycophorin B and residues 40-99 are derived from glycophorin A. Dantu is associated with very weak s antigen, a protease-resistant N antigen and either very weak or no U antigen. (wikipedia.org)
  • base
  • Both sequences are thought to be essential for base pairing during the initiation and resolution steps of the gene conversion process required for mating‐type switching. (embopress.org)
  • linear
  • Therefore, counterselection against the cells containing the marker needs to be performed in order to identify the cells that have successfully incorporated the linear DNA into the target sequence. (wikipedia.org)