• The results indicated that 2 of the fragments were homologous to the gene encoding the precursor of terminal protein (pTP) of adenoviruses. (cdc.gov)
  • AB302971) were homologous to the gene encoding the precursor of protein VI (pVI) of adenoviruses. (cdc.gov)
  • Phylogeny of adenoviruses based on analysis of partial amino acid sequences of DNA polymerase protein. (cdc.gov)
  • The amino acid is added to a growing protein during the process of translation. (wikibooks.org)
  • This research confirmed that deletion of some regions of the secretory signal sequence significantly improved the efficiency of HIP protein expression in P. pastoris . (springeropen.com)
  • One of these, derived from a patient with rapid CD4 cell decline, contained an amino acid substitution in a highly conserved endocytosis signal (Y721C), as mediated virus entry with very poor efficiency, although they did not contain sequence changes predicted to alter protein function. (duke.edu)
  • The miniF plasmid C protein: sequence, purification and DNA binding. (shengsci.com)
  • C protein binds strongly to pifO, and weakly to sequences in the ori-1 region. (shengsci.com)
  • Deep sequencing of RNA molecules cross-linked to immunoprecipitated protein particles (CLIP-seq) revealed. (shengsci.com)
  • Aftertranscription, these fragments guide protein complexes that target foreign nucleic acids and promote their degradation. (pjmonline.org)
  • This invention provides an APP-cleaving protein and related nucleic acid compounds. (everypatent.com)
  • Researchers hypothesize that abnormal accumulation of this 4 Kd protein in the brain is due to cleavage of a larger precursor protein, called amyloid precursor protein (APP). (everypatent.com)
  • While all coronaviruses contains these proteins, a subset (HEV, HCV-OC43 and BCV) is now recognized to possess an additional glycopolypeptide (gp65), which is unrelated to S or M. N protein The number of amino acids in the N protein has been determined by cloning and sequencing for MHV strains A59 (Armstrong et al. (pdfroom.com)
  • Highly specific and effective gene silencing of any disease can be achieved by an accurate knowledge of the target mRNA sequence and rational design of its complementary antisense agents for the downregulation of its protein message. (genomicglossaries.com)
  • Complementary DNA copies of select agent viral genomes, including reverse genetics systems, are not regulated because they would first need to be transcribed into RNA, then translated into protein, and therefore would not be an immediate precursor to the virus. (selectagents.gov)
  • The viral core protein retains protease activity, needed for cleavage from a protein precursor. (virology.ws)
  • Of note, functionally-relevant differences between humans and chimpanzees are observed regarding the 3 main components of this pathway: i) the protein sequences of immunes TFs binding MER41 LTRs, ii) the insertion sites of MER41 LTRs in the promoter regions of ID-associated genes and iii) the protein sequences of the targeted ID-associated genes. (biorxiv.org)
  • Genetic engineering of the processing site of D1 precursor protein of photosystem II reaction center in Chlamydomonas reinhardtii. (bio.net)
  • All recognized mutations for AD are associated with increased deposition of amyloid-beta (Abeta), a peptide fragment comprising 39-43 amino acids that derive from the catabolism of the amyloid precursor protein (APP) molecule. (medscape.com)
  • The proposed method is novel because it works independently of protein sequence databases and without any prior knowledge about modifica- tions. (lu.se)
  • The matching is com- plicated by the fact that there are protein modifications and the sequence databases store the unmodified sequences. (lu.se)
  • PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)
  • RNA sequences that serve as templates for protein synthesis. (bvsalud.org)
  • CRISPR/Cas systems constitute a widespread class of immunity systems that protect bacteria and archaea against phages and plasmids, and commonly use repeat/spacer-derived short crRNAs to silence foreign nucleic acids in a sequence-specific manner. (nature.com)
  • Among others, these conditions include the invasion of foreign nucleic acids such as bacteriophages and plasmids. (pjmonline.org)
  • A unique feature of the CRISPR-Cas system is that it provides adaptive and hereditary immunity against foreign nucleic acids, mim-icking in some ways the immune systems of eukaryotes. (pjmonline.org)
  • In addition, a phylogenic tree based on amino acid sequences indicated that RV1 belongs to family Adenoviridae , genus Mastadenovirus ( Figure ). (cdc.gov)
  • The 85 amino acid sequences of α-factor consisted of the 19 amino acids as pre-region followed by the 66 amino acids as a pro-region with three N-linked glycosylation sites at the amino acid numbers 23, 57, 67, and terminated by the Kex2 endo-proteinase cleavage site [ 23 , 24 ]. (springeropen.com)
  • FIGS. 8A-C depict amino acid sequences of wild-type and variant AAV capsids. (justia.com)
  • Analysis of their deduced amino acid sequences revealed several important differences from prototypic subtype B strains, including changes in the number and distribution of cysteine residues, substantial length differences in hypervariable regions, and premature truncations in the gp41 domain. (duke.edu)
  • The naturally available ribozymes are known to catalyze a narrow range of reactions such as phosphoryl transfer in nucleic acids and peptide bond formation in proteins. (jove.com)
  • The present article is based on the large amount of sequence data accumulated in these years and focuses on the viral nucleic acids and proteins and their function. (pdfroom.com)
  • It seems that not until the technologies for working with nucleic acids and proteins are better integrated will their researchers be more connected than they are now. (genomicglossaries.com)
  • rRNA genes contain regions of variable DNA sequence that are unique to the species carrying the … Ribosomal RNA is transcribed from ribosomal DNA (rDNA) and then bound to ribosomal proteins to form small and large ribosome subunits. (printerresource.com)
  • We have previously discussed the idea that viruses originated from selfish genetic elements such as plasmids and transposons when these nucleic acids acquired structural proteins (see A plasmid on the road to becoming a virus ). (virology.ws)
  • Missing from these hypothesis is how nucleic acids became virus particles - that is, how they acquired structural proteins. (virology.ws)
  • An analysis of the sequence an structure of major virion proteins has identified likely ancestors in cellular proteins. (virology.ws)
  • The extra sequences at the N-termini of viral jelly roll capsid proteins, involved in recognizing the viral genome, likely evolved after the capture of these proteins from cells. (virology.ws)
  • Some researchers have reported that XNAs containing specific sequences can act as enzymes and bind to proteins. (astrobiology.com)
  • Serum amyloid A and alpha-1 acid glycoprotein are transport proteins, and fibrinogen is a coagulation factor. (msdmanuals.com)
  • Antibodies recognize specific configurations (epitopes, or antigenic determinants) on the surfaces of antigens (eg, proteins, polysaccharides, nucleic acids). (msdmanuals.com)
  • Efforts to mimic nature typically utilize amino acids and amino acid derivatives to form peptidomimetics having amide bonds.Presented here is a new class of synthetic sequence-defined polymers which do not use peptide bonds. (pnnl.gov)
  • This sequential reactivity provides advancement over prior methods used to form conventional sequence-defined peptide polymers because it provides completely new polymeric architectures, as compared to conventional peptide, peptoid, or nucleic acid polymers. (pnnl.gov)
  • The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
  • The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)
  • Such methods generally comprise providing a cell with one or more precursor nucleic acid sequences that encode two or more RNA molecules. (genomeweb.com)
  • Nature uses sequence-defined polymers such as polypeptides to create biomaterials, encode information, perform biocatalysis, participate in molecular recognition, and shuttle species across membranes. (pnnl.gov)
  • These nucleic acid polymers encode for the all of the materials an organism needs to live in the form of genes. (wikibooks.org)
  • Nucleic acids that encode for the genomes of select agent bacteria or fungi, including chromosomal, recombinant, or synthetic DNA. (selectagents.gov)
  • Nucleic acids that encode for toxins not subject to the select agent regulations but derived from regulated select agents. (selectagents.gov)
  • Nucleic acids that encode for toxins and the genomes of select agents that have been excluded from regulation under section 3(e) of the select agent regulations. (selectagents.gov)
  • Moreover, transiently expressed glycoprotein precursor molecules varied considerably in both size and carbohydrate content. (duke.edu)
  • RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
  • More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)
  • Those RNA nucleic acid sequences are taken up by other cells in the organism and act as signalling molecules. (virology.ws)
  • Nagoya University scientists in Japan have demonstrated how DNA-like molecules could have come together as a precursor to the origins of life. (astrobiology.com)
  • Before this stage, the pre-RNA world may have been based on molecules called xeno nucleic acids (XNAs). (astrobiology.com)
  • The structural and sequence features on pre-miRNA which can facilitate position and efficiency of cleavage are not well known. (biomedcentral.com)
  • 2013). The proteomic component is responsible for the incorporation of new template sequences, processing them into a form that enables base pairing with target nucleic acids, as well as for scanning and cleavage of target DNA or RNA. (pjmonline.org)
  • Profesora Wacława Szybalskiego przyznano dwie równorzędne nagrody: dr Julia Staręga-Rosłan z Zakładu Biomedycyny Molekularnej, z Instytutu Chemii Bioorganicznej PAN w Poznaniu Starega-Roslan J, Galka-Marciniak P, Krzyzosiak W.J. Nucleotide sequence of miRNA precursor contributes to cleavage site selection by Dicer. (fpws.pl)
  • Functional analyses of ectopically expressed copies of the variant miRNA precursors demonstrate loss of function, gain of function, and altered expression levels. (genomeweb.com)
  • The miR-129 microRNA precursor is a small non-coding RNA molecule that regulates gene expression. (wikipedia.org)
  • A first RNA molecule comprises a double-stranded sequence, which includes a guide strand sequence that is complementary to a portion of an mRNA transcript encoded by the target gene. (genomeweb.com)
  • Preferably, the second guide strand sequence comprises one or more bases that are mismatched with a nucleic acid sequence of the mRNA transcript encoded by the target gene. (genomeweb.com)
  • We have determined the nucleotide sequence of the C gene. (shengsci.com)
  • Nucleotide sequencing of the M gene of MHV-A59 and MHV-JHM (Armstrong et al. (pdfroom.com)
  • Distel, D. L., DeLong, E. F., and Waterbury, J. Epub … Bacterial Identification by 16S rRNA Gene Sequence Analysis Background All bacteria contain 16S ribosomal RNA (rRNA) genes of approximately 1500 base pairs (bp) in length. (printerresource.com)
  • For purposes of 18 U.S.C. § 175c, "variola virus" is defined as "a virus that can cause human smallpox or any derivative of the variola major virus that contains more than 85 percent of the gene sequence of the variola major or the variola minor virus. (selectagents.gov)
  • Nucleotide sequence of the PetM gene encoding a 4 kDa subunit of the cytochrome b6f complex from Chlamydomonas reinhardtii. (bio.net)
  • The gene , GNRH1 , for the GnRH precursor is located on chromosome 8 . (wikidoc.org)
  • We developed number of models using various types of features and achieved maximum accuracy of 66% using binary profile of nucleotide sequence taken from 5p arm of hairpin. (biomedcentral.com)
  • Small ribozymes are usually 50 to 150 nucleotides long and are self-cleaving nucleotide sequence motifs. (jove.com)
  • REFERENCE 1 (bases 89 to 309) AUTHORS Miwa,T. and Kamada,S. TITLE The nucleotide sequence of a human smooth muscle (enteric type) gamma-actin cDNA JOURNAL Nucleic Acids Res. (bio.net)
  • A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
  • They synthesized fragments of acyclic (non-circular) L-threoninol nucleic acid (L-aTNA), a molecule that is thought to have existed before RNA came to be. (astrobiology.com)
  • The mature sequence is excised by the Dicer enzyme from the 5' arm of the hairpin. (wikipedia.org)
  • They also found that the amplicons were similar in size distribution to the parent cDNA and showed sequence heterogeneity. (genomicglossaries.com)
  • Therefore, the level of Cab mRNA, as measured by the presence of sequences hybridizing to a cDNA probe, does not limit the rate of chlorophyll accumulation after transfer of pea seedlings to white light. (ncsu.edu)
  • The previous studies successfully expressed recombinant human insulin precursors (HIP) in Pichia pastoris truncated and full-length α-factor recombinant clones. (springeropen.com)
  • Phylogenetic analyses of full-length env sequences indicated that the panel included members of all major sequence subtypes of HIV-1 group M (clades A to G), as well as an intersubtype recombinant (F/B) from an infected individual in Brazil. (duke.edu)
  • In addition, all subtype E and three subtype G viruses initially classified on the basis of partial env sequences were found to cluster in subtype A in the 3' half of their gp41 coding region, suggesting that they are also recombinant. (duke.edu)
  • However, no viral nucleic acid sequence was detected from an RNA sample in the RV1-infected BKT1 cells. (cdc.gov)
  • For detection of viral DNA, we developed a system for rapid determination of viral DNA sequences (RDV-D) by minor modification to the RDV system for RNA viruses ( 2 - 4 ). (cdc.gov)
  • Complementary DNA made from regulated select agent nucleic acids ( only the positive strand RNA form of the viral genome is regulated). (selectagents.gov)
  • The adaptive immune response produces a diversity of different antibodies (on the order of 10 11 unique sequences in a single individual), each of which is capable of binding to a different target epitope. (justia.com)
  • Further, even in the case of two patients that make autoantibodies against the same target, the sequences of these antibodies will be different. (justia.com)
  • RNA which contains an intron sequence that has an enzyme- like catalytic activity. (genomicglossaries.com)
  • The paper, "Nonenzymatic polymerase-like template-directed synthesis of acyclic L-threoninol nucleic acid," was published online in the journal Nature Communications on February 5, 2021 at DOI: 10.1038s41467-021-21128-0. (astrobiology.com)
  • They also made a longer L-aTNA with a nucleobase sequence that complemented the sequences of the fragments, similar to how DNA strands match up. (astrobiology.com)
  • Ribonuclease P is found in some bacteria and processes precursor tRNA to generate a mature 5' end. (jove.com)
  • The genomic component of the CRISPR-Cas system is formed by a series of variable spacers, which in some cases share sequence similarity with viruses, plasmids, or bacteria. (pjmonline.org)
  • Nucleic acids or genetic elements that cannot produce infectious forms of any of the regulated select agent viruses. (selectagents.gov)
  • DNA is a nucleic acid which is capable of duplicating itself via the enzyme known as DNA polymerase. (wikibooks.org)
  • 87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
  • FIND) has partnered with Cepheid, Inc. (Sunnyvale, CA) and the University of Medicine and Dentistry of New Jersey (UMDNJ, Newark, NY) to develop a TB-specific automated, cartridge-based nucleic amplification assay (Xpert MTB/RIF) based on the GeneXpert multi-disease platform, currently unique in its simplification of molecular testing with fully integrated and automated sample preparation, amplification and detection required for real-time polymerase chain reaction. (bvsalud.org)
  • Scholars@Duke publication: Molecular cloning and analysis of functional envelope genes from human immunodeficiency virus type 1 sequence subtypes A through G. The WHO and NIAID Networks for HIV Isolation and Characterization. (duke.edu)
  • To generate a more representative panel of genetically diverse envelope genes, we PCR amplified, cloned, and sequenced complete gp160 coding regions of 35 primary (peripheral blood mononuclear cell-propagated) HIV-1 isolates collected at major epicenters of the current AIDS pandemic. (duke.edu)
  • The CcpA regulon comprises genes involved in sugar uptake, fermentation and amino acids metabolism, confirming the role of CcpA as a link between carbon and nitrogen pathways. (studyres.com)
  • Using combination of chromatin immunoprecipitation and genome sequence analysis, we detected 55 CcpA binding sites corresponding to 140 genes directly controlled by CcpA. (studyres.com)
  • Here, we present evidence that the promoter regions of intellectual disability-associated genes are uniquely enriched in LTR sequences of the MER41 HERVs. (biorxiv.org)
  • Thus, multiple reactive sites are utilized that can be sequentially acted upon in a pre-determined, well-controlled manner to create molecular precursors or submonomers. (pnnl.gov)
  • In DNA a sequence of three bases, which is called a codon, is responsible for the encoding of a single amino acid. (wikibooks.org)
  • In Genbank the codon start is /codon_start=1 for HUMACTSG7 and the translated sequence is different. (bio.net)
  • Different combination in percentage of the individual 20 amino acid mix codons can be ordered to be synthesized at the same codon position. (genelink.com)
  • Thus at a single codon position we can spike it with a customer desired percentage of a particular codon or number of amino acid codon. (genelink.com)
  • Some of these sites are located near the initiation region for the 45 S rRNA precursor, while others are associated with a series of short direct repeats 5′ to the 45 S rRNA initiation site. (ncsu.edu)
  • A. Ribosomal RNA analysis StructRNAfinder - predicts and annotates RNA families in transcript or genome sequences. (printerresource.com)
  • 1. A purified protease which comprises the amino acid sequence SEQ ID NO:1, or a functional equivalent thereof produced by conservative amino acid alterations which result in a protease which exhibits the same biological, biochemical, chemical, physical and structural properties of the protease of SEQ ID NO:1. (everypatent.com)
  • A4 is often an aspartic acid (Asp), indicating that a protease which cleaves between the methionine (Met) at position 596 (Met.sub.596 using the numbering system according to Kang J. et al. (everypatent.com)
  • Kunitz-like domain"--A protease inhibitor similar to soybean trypsin inhibitor or a nucleic acid sequence encoding a protease inhibitor which is similar to the soybean trypsin inhibitor. (everypatent.com)
  • In 2004 Rawlings and colleagues introduced a classification of protease inhibitors based on similarities detectable at the level of amino acid sequence. (wikipedia.org)
  • DNA is a long chain of linear polymers containing deoxyribose sugars and their covalently bonded bases known as nucleic acids. (wikibooks.org)
  • It has also been demonstrated that STAT1 (Signal Transducer And Activator Of Transcription 1), a transcription factor (TF) crucially involved in the IFNγ pathway, binds consensus sequences that, in humans, are located with a high frequency in the LTRs (Long Terminal Repeats) of the MER41 family of primate-specific HERVs (Human Endogenous Retrovirus). (biorxiv.org)
  • High-throughput RNA sequencing (RNA-seq) and bioinformatics were used to identify the potential microorganisms. (asm.org)
  • Nucleic Acids Research, 16(22), 10511–10528. (ncsu.edu)
  • Several methods exist for the prediction of precursor miRNAs (pre-miRNAs) in genomic or sRNA-seq (small RNA sequences) data produced by NGS (Next Generation Sequencing). (biomedcentral.com)
  • We provide a simpler and much faster method with very reasonable sensitivity and precision, which can be applied without special adaptation to the prediction of both animal and plant pre-miRNAs, using as input either genomic sequences or sRNA-seq data. (biomedcentral.com)
  • The glycolate pathway also generates C 1 compounds and serves as the generator of precursors for nucleic acid biosynthesis. (exama2z.in)
  • Sequence analysis of the amplified product (DDBJ/EMBL/GenBank accession no. (cdc.gov)
  • Immunoglobulin Heavy Chain High-Throughput Sequencing in Pediatric B-Precursor Acute Lymphoblastic Leukemia: Is the Clonality of the Disease at Diagnosis Related to Its Prognosis? (frontiersin.org)
  • High-throughput sequencing (HTS) of the immunoglobulin heavy chain ( IgH ) locus is a recent very efficient technique to monitor minimal residual disease of B-cell precursor acute lymphoblastic leukemia (BCP-ALL). (frontiersin.org)
  • B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is the most common pediatric neoplasm ( 1 , 2 ). (frontiersin.org)
  • ConoServer, a database for conopeptide sequences and structures. (conoserver.org)
  • It also reveals the sequences of clonal rearrangements, therefore, the multiclonal structure, of BCP-ALL. (frontiersin.org)
  • High-risk types (e.g., types 16 and 18) can cause low-grade cervical cell abnormalities, high-grade cervical cell abnormalities that are precursors to cancer, and cancers ( 5 - 7 ). (cdc.gov)
  • Nucleic acid mapping to Plasmodium was also used on the patient samples, confirming results obtained with an antigen-based rapid diagnostic test (RDT) conducted in the field laboratories. (asm.org)
  • Policy statement: automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance: Xpert MTB/RIF system. (bvsalud.org)
  • Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). (abcam.com)
  • There is also the phenomenon of exosomes that package nucleic acids in a membrane envelope. (virology.ws)
  • The present invention relates to methods for isolating the sequences of an antibody that reacts with a disease related antigen, e.g., an autoantigen, without knowing the identity of the antigen (sequence or structural epitope) a priori. (justia.com)
  • In photorespiration, glycolic acid (glycolate), a two-carbon product of photosynthesis is released from chloroplasts and oxidized into and H 2 O 2 by a peroxisomal enzyme called glycolic acid oxidase. (exama2z.in)
  • These regions are interspaced with repeat sequences that are identical or almost identical within a single CRISPR cassette. (pjmonline.org)
  • Earlier studies systematically analyzed sequence variations in human pri-miRNAs/pre-miRNA and experimentally discovered that single nucleotide polymorphisms (SNPs) in miR-125a obstruct the processing of pri-miRNA to pre-miRNA [ 16 ]. (biomedcentral.com)