Sequence genetic complementation test. Medical search. Frequent questions

We have cloned and sequenced polyketide synthase (PKS) genes from the aclacinomycin producer Streptomyces galilaeus ATCC 31,615. (nih.gov)
The sequenced 13.5-kb region contained 13 complete genes. (nih.gov)
Their organization as well as their protein sequences showed high similarity to those of other type II PKS genes. (nih.gov)
Furthermore, these FGF ligands are not regulated solely by differential expression, but the sequences of these linked genes have evolved to serve different functions. (biologists.com)
We have sequenced the remainder of the nopaline-specific T-DNA (containing genes a to d) and acs to iaaM. (cnrs.fr)
The restoration after fusion of normal RRS levels allows the classification of patients into different complementation groups (i.e., they are defective in different genes) whereas the maintenance of impaired RRS levels indicates that the patients are in the same complementation group (i.e., they are defective in the same gene). (geneskin.org)
In parallel to the classical complementation assay, genetic analysis may be carried out also by analyzing the level of RRS in patient's cells after microinjection of plasmid vectors expressing the UVSS genes or after infection with recombinant lentivirus or adenovirus vectors (Jia et al. (geneskin.org)
A new diagnostic approach based on the direct search for mutations in a panel of relevant genes has been recently developed by the use of next generation sequencing technology (Calmels et al. (geneskin.org)
Using the Drosophila nephrocyte as a genetic screen platform, most genes involved in endocytosis and cell trafficking were screened, and the key components were identified of the cell trafficking pathway required for SD protein endocytosis and recycling. (sdbonline.org)
Researchers from Canada, Sweden, and the US present findings from a functional screen of nearly 200 genetic variants in 22 genes implicated in human disease in the budding yeast assay Saccharomyces cerevisiae . (genomeweb.com)
After looking at how well human disease genes rescued the activity of temperature sensitive versions of orthologous yeast genes, the team came up with a set of 26 functional assays for evaluating human disease gene activity, including assays based on 19 complementation relationships not reported previously. (genomeweb.com)
The chapter considers why scientists are unable to name every gene that is discovered, and explains how genetic analysis uses the properties of genes as an approach to solve or dissect a complex biological problem. (oxfordsciencetrove.com)
Transcriptional profiling of these candidate GST genes and key anthocyanin biosynthetic pathway genes (PAL, CHS, DFR, and UFGT) in cell suspensions and grape berries against anthocyanin accumulation demonstrated strong positive correlation with two sequences, VvGST1 and VvGST4, respectively. (oregonstate.edu)
For example, in the short term, inheriting copies of both parents' genes usually provides good insurance against mutations that might kill the function of one of those gene copies - a process called complementation," Fitch continues. (agribusinessinfo.com)
Their results showed that D. pachys fuses the six chromosomes of its ancestor into a single chromosome and skips the first division of meiosis, where genes are recombined, so that its offspring keeps the high genetic diversity of the parents. (agribusinessinfo.com)

PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING. (lookformedical.com)
At the organismal level, evolutionary distance is defined by genome-wide sequence homology between species. (frontiersin.org)
Reinventing potato from a clonally propagated tetraploid to a true seed-propagated diploid has the potential to considerably accelerate genetic improvement, and would enable the genome design of a crop that has been highly recalcitrant to the use of molecular breeding and genomics approaches 3 , 6 , 7 . (nature.com)
Here we report genome sequences and analyses of 44 diploid potatoes, as well as 2 species in the Etuberosum section. (nature.com)
Whole genome sequencing technology and complementation analysis were used to determine that the stl83 phenotype is the result of a missense mutation in the gene actin-related protein 10 (actr10), which encodes a critical component of the dynactin complex. (grantome.com)
The researchers decided to sequence the genome of D. pachys to test how the single chromosome was structured, whether by loss or by fusion of multiple ancestral chromosomes. (agribusinessinfo.com)
In line with its global importance, an initiative to sequence the oil palm genome was carried out successfully, producing huge amounts of sequence information, allowing SNP discovery. (mpob.gov.my)

Specific gene sequencing testing to define the causative genetic mutation is strongly recommended, though not required. (sparkcures.com)
Must have genetic testing confirming a mutation in the Shwachman-Bodian-Diamond syndrome (SBDS) gene, and/or classic clinical features of Shwachman-Diamond Syndrome, including pancreatic insufficiency, musculoskeletal anomalies, and endocrinopathies ii. (sparkcures.com)
Using a forward genetic screen, we identified the recessive boygirl ( byg ) mutation. (plos.org)
2007). Here, we report the unusual genetic behavior of a quantitative gain-of-function CNGC mutation ( brush ) in Lotus japonicus resulting in a leaky tetrameric channel. (elifesciences.org)
We sequenced the genomic DNA spanning BRI1 in the cp3 and found that cp3 has a point mutation in the region encoding the 13th LRR of BRI1, resulting in a change from serine to phenylalanine (S399F). (biomedcentral.com)
Traditionally, monogenic disorders have been considered as relatively straightforward genetic conditions, where a mutation in a single gene is sufficient to cause the observed phenotype. (e-kjgm.org)

Sequence analysis of the affected gene is usually carried out at the cDNA and genomic DNA levels. (geneskin.org)
D) Schematic representation of pGS-3700 targeting plasmid containing a 3723 bp fragment viral genomic sequence including the viral cyclin from positions 101,654 and 105,377 (shown in reverse orientation here) in the pGS284 BAC recombination vector, and modifications to create the pGS3700. (galeriaespacio48.com)
The gHV68 cyclin and M11 coding sequences are indicated, and gHV68 genomic sequence is indicated MC-Val-Cit-PAB-carfilzomib by dashed line. (galeriaespacio48.com)
G-quadruplex (G4) DNA, an alternate structure formed by Hoogsteen hydrogen bonds between guanines in G-rich sequences, threatens genomic stability by perturbing normal DNA transactions including replication, repair, and transcription. (nih.gov)
With the advent of advanced genomic technologies and large-scale sequencing projects, it has become evident that the genetic architecture of many disorders is more complex than previously anticipated. (e-kjgm.org)

Patients and consumers with specific questions about a genetic test should contact a health care provider or a genetics professional. (beds.ac.uk)
The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development. (lookformedical.com)

We have cloned the RAD6 homolog from Drosophila melanogaster and find that its encoded protein displays a very high degree of identity in amino acid sequence with the homologous RAD6 proteins from the two divergent yeasts, S. cerevisiae and Schizosaccharomyces pombe, and from human. (eur.nl)
Here, we tested whether these RNA-binding proteins act jointly in a common mechanism. (bvsalud.org)
These proteins underwent Edman sequencing and mass spectrometry fingerprinting, with the resultant fragments aligned with predicted GSTs within public databases. (oregonstate.edu)
The ability of VvGST1 and VvGST4 to transport anthocyanins was confirmed in the heterologous maize bronze-2 complementation model, providing further evidence for their function as anthocyanin transport proteins in grape cells. (oregonstate.edu)
In chapter 2, by using CRISPR/Cas9 gene editing, genetic analysis and biochemical assays, I identified the differential pairings of typical NLR receptor SOC3 with atypical NLR proteins CHS1 or TN2 to guard the homeostasis of the E3 ligase SAUL1. (ubc.ca)

Next generation sequencing (NGS) of nucleic acid extracts from plants infected with unknown viruses is expected to yield information about the genomes of previously uncharacterized viruses without any background information on what viruses might be infecting the plant. (usda.gov)

The rimR 2 deletion and complementation assays were conducted to explore its role. (biomedcentral.com)

The newly sequenced worm, Diploscapter pachys , is a tiny, transparent, free-living roundworm and closely related to Caenorhabditis elegans , an organism commonly used for biomedical research. (agribusinessinfo.com)
Pictured is Diploscapter pachys (D. pachys), a newly sequenced roundworm that is approximately one-third of a millimeter long and one of a very few known animals that have only a single chromosome. (sciencebulletin.org)

Specific testing to define the subtype of Fanconi Anemia through genetic sequencing for causative mutations or complementation group studies is strongly recommended, though not required. (sparkcures.com)
https://www.cincinnatichildrens.org/service/d/diagnostic-labs/cytogenetics/fanconi-anemia 000 Complete the appropriate test requisition and have it signed by the referring physician. (beds.ac.uk)

The complementation assay is carried out by fusing fibroblasts of the patient under evaluation with fibroblasts mutated in either the CSA , CSB or UVSSA gene and by analyzing RRS levels at late times after irradiation in heterodikaryons. (geneskin.org)
Here, we develop a highly parallel protein-protein interaction sequencing (PPiSeq) platform that uses a novel double barcoding system in conjunction with the dihydrofolate reductase protein-fragment complementation assay in Saccharomyces cerevisiae . (nature.com)
A yeast complementation assay showed that heterologous expression of NcACBP rescued the phenotypic defects in Δacbp yeast, indicating of the binding activity of NcACBP in vivo . (biomedcentral.com)

The corresponding coding sequences were cloned, with heterologous expression in Escherichia coli used to confirm GST activity. (oregonstate.edu)

The long-term objective of this project is to develop effective means for the detection and identification of new and emerging plant viral and bacterial diseases of ornamentals, thus allowing growers to select pathogen-free or pathogen-indexed plants (tested for absence of specific pathogens) for propagation. (usda.gov)
A Pennsylvania State University-led team describes a strategy for sequencing mammalian Y chromosomes in a manner that's speedier and more affordable than bacterial artificial chromosome-based approaches used in the past. (genomeweb.com)

spMex67p, a 596-amino-acid-long protein, has considerable sequence similarity to the Saccharomyces cerevisiae Mex67p (scMex67p) and human Tap. (ox.ac.uk)
Comparison of the wheat PAB protein sequence with PABs from yeast and other species revealed that wheat PAB contained the characteristic features of all PABs, including four RNA binding domains each of which contained the conserved RNP1 and RNP2 sequence motifs. (oregonstate.edu)
The wheat PAB cDNA functionally complemented a pab1 mutant in yeast suggesting that, although the amino acid sequence of wheat PAB is only 47% conserved from that of yeast PAB, this monocot protein can function in yeast. (oregonstate.edu)

Identification of the defective gene is followed by sequence analysis to detect the type and location of the causing mutations. (geneskin.org)

Furthermore, the identification of viral sequences largely depends on similarity searches against reference databases. (cnrs.fr)

Additionally, this study revealed distinct genetic complementation groups of the mammalian cyclins, demonstrating that mammalian cyclins can fulfill the biochemical features of the v-cyclin in infection. (galeriaespacio48.com)

Definition of the gene responsible for the repair defect in UV S S patients is performed by complementation analysis based on somatic cell hybridization. (geneskin.org)

Here, we compared sequence and systematically mapped position of DNase I hypersensitive sites, acetylation status of histone H3/H4, and in vivo binding of transcription factors over approximately 120 kilobases flanking the human GATA1 gene and the corresponding region in mice. (ox.ac.uk)
Our study demonstrated that genetic ablation of NcACBP did not impair the survival and growth phenotype of N. caninum but enhanced its pathogenicity in mice. (biomedcentral.com)

Genetic complementation studies indicate that the Drosophila RAD6 homolog can functionally substitute for the S. cerevisiae RAD6 gene in its DNA-repair and UV-mutagenesis functions but cannot substitute in sporulation. (eur.nl)

From analysis of a limited number of conserved gene loci, sequence comparison has proved a robust and efficient way to locate cis-elements. (ox.ac.uk)
Sequence and functional analysis of the left-hand part of the Ŧ-region from the nopaline-type Ti plasmid, pTiC58. (cnrs.fr)
Detailed protocols for evaluation of UDS, RRS and viability after UV irradiation and for complementation analysis are reported in the corresponding section in Cockayne syndrome card. (geneskin.org)
Further analysis of two overlapping deficiency lines and mutant alleles led to identification of genetic interactions between the CDK8-CycC pair and the components of the Decapentaplegic (Dpp, the Drosophila homolog of TGFβ, or Transforming Growth Factor-β) signaling pathway. (sdbonline.org)
Finally, all BAC recombinants for cyclin analysis were transfected into Vero-Cre cells for removal of BAC sequences prior to infection of 3T12 fibroblasts for generation of virus stocks. (galeriaespacio48.com)
Co-dominant simple sequence repeat (SSRs) are popular DNA markers, widely applied in oil palm genetic studies, especially in genetic mapping, marker-trait association, diversity analysis and detecting illegitimacy. (mpob.gov.my)
OPSRI is a well-structured database that can expedite marker development and reduce redundancy in the on-going efforts at developing DNA markers for genetic analysis of oil palm. (mpob.gov.my)

D. pachys overcomes the disadvantages of asexual reproduction by maintaining genetic variation, and with it, complementation," explains Fitch. (agribusinessinfo.com)
Thus, the mystery of its longevity seems largely resolved: D. pachys overcomes the disadvantages of asexual reproduction by maintaining genetic variation, and with it, complementation," explains Fitch. (sciencebulletin.org)

Must have chromosomal breakage (stress) testing performed demonstrating increased sensitivity to DNA damage caused by mitomycin C (MMC) or diepoxybutane (DEB) iii. (sparkcures.com)

Here, we employed a Drosophila model to test links between glycosphingolipids, neurotransmission and neurodegeneration. (bvsalud.org)
To identify upstream regulators or downstream effectors of CDK8, a dominant modifier genetic screen was performed in Drosophila based on the defects in vein patterning caused by specific depletion or overexpression of CDK8 or CycC in developing wing imaginal discs. (sdbonline.org)

A snc1-influencing plant E3 ligase reverse (SNIPER) genetic screen identified two snc1-suppressors, SNIPER1 and SNIPER2, both of which completely suppress snc1-mediated autoimmunity upon overexpression. (ubc.ca)

Chromosome breakage can be ordered sequentially along with molecular genetic testing. (beds.ac.uk)

We now confirm those findings, as complementation of a csrS mutant restored Mg(2+)-dependent gene regulation. (lu.se)
The mutant saul1-1 displays seedling lethality from its autoimmunity, which makes it a suitable background for genetic screens. (ubc.ca)

In this study, we investigated the genetic architecture controlling grain free amino acid composition and its relationship to other traits in a Robigus × Claire doubled haploid population. (rothamsted.ac.uk)

and, (3) Characterize genomes of bacteria of major significance to ornamental and nursery crops to develop diagnostic tests for accurate pathogen detection. (usda.gov)
The framework has a supporting table with information on the diagnostic tests, biomarkers and proposed cutoffs, characteristics, and feasibility of collecting the myriad information that can help elucidate the anemia etiology. (cdc.gov)

To uncover new regulators of myelination, our lab performed a large-scale forward genetic screen in zebrafish. (grantome.com)

In insects, we compared sequences of small and long RNAs, demonstrating that viral sequences are enriched in the small RNA fraction. (cnrs.fr)
We also noted that the small RNA size profile is a unique signature for each virus and can be used to identify novel viral sequences without known relatives in reference databases. (cnrs.fr)

Chromosomal breakage syndromes are a group of genetic disorders that are typically transmitted in an autosomal recessive mode of inheritance. (medscape.com)

Biotechnology - new and sometimes controversial branch of biology that studies the manipulation of living matter, including genetic modification and synthetic biology. (wikipedia.org)
We present relevant studies and highlight the importance of adopting a broader genetic approach in understanding the complex genetic architecture of these conditions. (e-kjgm.org)

The causes of anemia are classified as non-nutritional (for example, because of infection, inflammation, blood loss, or genetic disorders) or nutrition-specific (for example, because of deficiencies of iron, vitamin A, riboflavin, vitamin B(12), or folate). (cdc.gov)

Samples were tested for the presence of various arboviral RNA genomes by using the TaqMan quantitative reverse transcription-PCR (qRT-PCR) technology and specific primers and probes (protocols available upon request to the corresponding author). (cdc.gov)

A team of scientists has sequenced, for the first time, a tiny worm that belongs to a group of exclusively asexual species that originated approximately 18 million years ago - making it one of the oldest living lineages of asexual animals known. (agribusinessinfo.com)

More generally, mouse human sequence comparison may fail to identify all cis-elements. (ox.ac.uk)
In this regard, emerging technologies of chimeric human organ production via blastocyst complementation (BC) holds great promise. (frontiersin.org)

For patients with a family history of colorectal cancer or advanced adenoma that was diagnosed before age 60 years in one first-degree relative or at any age in two first-degree relatives, testing should begin with colonoscopy at an age10 years younger than the youngest age at diagnosis of a first-degree relative, or age 40, to be repeated every 5 years. (medscape.com)

In Aim 1, I will characterize defects observed in actr10 zebrafish mutants in order to determine the stage at which actr10 regulates myelination and test whether the deficits are glial cell autonomous. (grantome.com)

1. Patients must have testing (eg. (sparkcures.com)
For patients at average risk, testing with a tier 1 test should begin at age 45 years for African Americans and at age 50 for patients of all other races. (medscape.com)
In patients with one first-degree relative with colorectal cancer, advanced adenoma, or an advanced serrated lesion diagnosed at age 60 or older, screening should begin with a tier 1 test at age 40 and continue at the same intervals as in average-risk patients. (medscape.com)
Colonoscopy screening should be discontinued in patients aged 75 or older with prior negative screening tests or whose life expectancy is less than 10 years, or in those 85 years or older without prior screening. (medscape.com)

Graded scoring techniques that assign a fitness to each colony have been employed elsewhere, for example, in synthetic genetic interaction screens 16 . (nature.com)

cycFdr (founder) plasmid for uniform insertion of cyclin coding sequences. (galeriaespacio48.com)

When a laboratory updates a registered test, a new version number is assigned. (beds.ac.uk)
Services are registered in the GTR with the registration of the laboratory, and not as a test. (beds.ac.uk)

Our results indicated that all the pretreatment inhibitors tested in this study had a detrimental effect on both Z. mobilis and S. cerevisiae , and vanillin had the most inhibitory effect followed by furfural and then HMF for both Z. mobilis and S. cerevisiae . (biomedcentral.com)
This study also characterised a large number of expressed sequence tags (EST)-SSRs using an in-silico approach. (mpob.gov.my)

Organisms5

Chemicals and Drugs3

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Phenomena and Processes18

Information Science3

Health Care1

Genes15

Genome7

Mutation6

Genomic5

Genetics2

Proteins5

Nucleic Acid1

Assays1

Newly sequenced2

Fanconi Anemia2

Assay3

Heterologous expression1

Bacterial2

Protein3

Mutations1

Similarity1

Mammalian1

Hybridization1

Mice2

Mutagenesis1

Analysis7

Pachys2

Sensitivity1

Drosophila2

Plant1

Chromosome1

Mutant2

Amino1

Diagnostic Tests2

Forward genetic screen1

Viral2

Breakage1

Studies2

Disorders1

Genomes1

Group1

Human2

Relatives1

Regulates1

Patients4

Screens1

Cyclin1

Laboratory2

Study2