Sequence contigs 4 chromosomes human 4 5. Medical search. Frequent questions

The physical map provides a framework for assembly of genome sequence and information for localization of genes that are difficult to find through positional cloning. (frontiersin.org)
Identification of 40 genes on a 1-Mb contig around the IL-4 cytokine family gene cluster on mouse chromosome 11. (jax.org)
FDY originated 2 million years ago from a duplication of a contiguous autosomal segment of 11 kb containing five genes that inserted into the Y chromosome. (speakerdeck.com)
Consistent loss of DNA sequences from several regions on the short arm of human chromosome 3 has suggested that multiple tumor suppressor genes reside on chromosome 3p in various types of cancer cells. (elsevierpure.com)
We have identified five new genes from these two contigs and there is evidence to suggest that several additional genes reside within these cosmid contigs. (elsevierpure.com)
Of the 155 markers present in the two contigs, 141 (33 genes and 108 STS) were generated and mapped in this study. (uaeu.ac.ae)
Considering limited representation of the region in the latest version of the horse whole genome sequence EquCab2, the dense map developed in this study will prove useful for the assembly and annotation of the sequence data on ECA21 and will be instrumental in rapid search and isolation of candidate genes for traits mapped to this region. (uaeu.ac.ae)
Here we show that the 37-megabase (Mb) genome of A. oryzae contains 12,074 genes and is expanded by 7-9 Mb in comparison with the genomes of Aspergillus nidulans 5 and Aspergillus fumigatus 6 . (nature.com)
The blocks of A. oryzae -specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. (nature.com)
Distribution and expression of the genes on chromosome 1. (nature.com)
Screening the porcine CHORI-242 BAC library with a full-length AKR1C4 cDNA identified 7 positive clones and sample sequencing of 5 BAC clones revealed 5 distinct AKR1C genes ( AKR1CL2 and AKR1C1 through 4 ), which mapped to 126-128 cM on SSC10. (biomedcentral.com)
Using the IMpRH 7000rad and IMNpRH2 12000rad radiation hybrid panels, these 5 genes mapped between microsatellite markers SWR67 and SW2067 . (biomedcentral.com)
Comparison of sequence data with the porcine BAC fingerprint map show that the cluster of genes resides in a 300 kb region. (biomedcentral.com)
In the human, an aldo-keto reductase (AKR) gene family (AKR1C) has been identified near the telomere on chromosome 10p15 that contains at least six aldo-keto reductase family 1, member C genes [ 11 ]. (biomedcentral.com)
The homologous region on mouse chromosome 13 contains a cluster of eight or nine AKR1C genes [ 12 ]. (biomedcentral.com)
These duplicated genes maintain a high degree of sequence similarity, but differ greatly in their substrate specificity and tissue expression patterns. (biomedcentral.com)
The human pregnancy-specific glycoprotein genes are tightly linked on the long arm of chromosome 19 and are coordinately expressed. (nih.gov)
and "As many as 100,000 distinct isoform transcripts could be produced from the 20,000 human protein-coding genes (Pan et al. (uncommondescent.com)
upstream1000.fa.gz - Sequences 1000 bases upstream of annotated transcription starts for RefSeq genes with annotated 5' UTRs. (ucsc.edu)
2. Users are free to use the data in scientific papers analyzing particular genes and regions if the providers of this data (the Rat Genome Sequencing Consortium) are properly acknowledged. (ucsc.edu)
For the vast majority of genes, the sequence in these regions was indeed finished. (bcgsc.ca)
We also identified several genes that do not appear to be protein coding, and generated 17 kb of novel transcript sequence data from re-sequencing 97 EST clones. (biomedcentral.com)
The candidate interval was shown to exhibit an extraordinary level of alternate splicing - 19 transcripts were identified for the 5 protein coding genes, but it appears that a significant portion (14/19) of these alternate transcripts did not have an open reading frame, hence their functional role is questionable. (biomedcentral.com)
Once the human and mouse genomes are done and the genes have been identified by comparison of the two genomes, much of the excitement will pass. (uthsc.edu)
Bets are actually being taken on the number of human genes, and the number ranges from 34000 to 153000. (uthsc.edu)
How can it be that we have 87% of the human genome sequence, but we cannot agree on the number of genes within a factor of 4 to 5? (uthsc.edu)
Because P450 genes are moderately long, about 10000 to 40000 bp, the short reads in phase 0 sequence cannot cover a whole gene. (uthsc.edu)
It is still difficult and hazardous to assemble genes from phase 1 sequence. (uthsc.edu)
Ten of them had P450 sequence on them, but there was evidence for five different subfamilies being present and probably 7 different genes. (uthsc.edu)
This was a phase two sequence with only two fragments and they were ordered, so it was now possible to assemble the genes correctly assuming that none of them fell in the single gap or ran off one end or the other. (uthsc.edu)
and he Began to use it to Identify genes welke are Expressed in the human brain. (multi-mania.be)
The NIH Office of Technology Transfer and Venter decided to use synthesis ESTs in an attempt to patent the genes they 'those given based on hun studies or mRNA expression in the human brain. (multi-mania.be)
A common approach to the mutational screening of candidate genes consists of sequencing their exons and immediate intron boundaries. (molvis.org)
However, since pathogenic mutations can sometimes be located deep within introns, as was recently shown for retinal degeneration genes as well [ 7 , 8 ], we decided to analyze the full MDH1 sequence. (molvis.org)
Radiation hybrid maps have a resolution power intermediate to that of the genetic and BAC contig maps and are also a powerful tool for the mapping of ESTs and genes by simple PCR. (biomedcentral.com)
This information was used to develop markers from chicken EST sequence data orthologous to genes in these human regions, in addition to the existing markers from the chicken chromosome 5 genetic map. (biomedcentral.com)
have analyzed the tef-1α sequences of F. equiseti , as well as the toxin-related genes PKS13 , PKS4, and TRI5 [26], and Kari has successfully cloned a protease gene [27]. (researchsquare.com)
Thus, about 100 human "twin genes", were found in the Arabidopsis genome, including, those responsible for such severe diseases as cystic, ﬁbrosis and breast cancer [50]. (centrocardiobogota.com)
With the human DNA probe, signals were detected in the major sites of the ribosomal genes on chromosomes 5I (7H) and 6I (6H) only when the chromosome preparations were obtained using an optimized technique with obligatory pepsin treatment followed by hybridization. (centrocardiobogota.com)
The above-mentioned ab, of repetitive sequences in plant genomes (up to 99%), makes difﬁcult the application of this technique for, mapping individual plant genes [40]. (centrocardiobogota.com)
Genes adjacent to pvtramp were identified in silico to determine the degree of similarity between the protein sequences encoded by equivalent chromosomic fragments in P. falciparum and Plasmodium knowlesi . (biomedcentral.com)
Using the latest version of the draft sequence, the differentially expressed genes were mapped to the pig genome. (biomedcentral.com)
This enabled co-location of DE genes and previously studied QTL regions, but the draft genome sequence is still incomplete and will contain many errors. (biomedcentral.com)
Genes Chromosomes Cancer 2008(1): 26-33 [ PubMed abstract ]. (lu.se)
Genes Chromosomes Cancer 2007(6): 517-21 [ PubMed abstract ]. (lu.se)
Genes Chromosomes Cancer 2006(6): 583-91 [ PubMed abstract ]. (lu.se)

Whole genome profiling (WGP) is a sequence-based physical mapping technology and uses sequence tags generated by next generation sequencing for construction of bacterial artificial chromosome (BAC) contigs of complex genomes. (frontiersin.org)
Sequencing of the A. oryzae genome was accomplished using the whole-genome shotgun (WGS) approach. (nature.com)
Phylogenetic analysis of the three aspergilli using the whole-genome data showed that A. nidulans branched off earlier than A. oryzae and A. fumigatus 5 . (nature.com)
human whole-genome. (scirp.org)
Using long-insert jumping libraries at 105X mean physical coverage and linked-read whole-genome sequencing from 10X Genomics, we document seven major SV classes at ~5 kb SV resolution. (biomedcentral.com)
In this study, the Illumina HiSeq 4000 and PacBio platforms were used to sequence and assemble the whole genome of Fusarium equiseti D25-1. (researchsquare.com)
Advances in whole-genome sequencing technologies have enabled numerous key discoveries. (researchsquare.com)
sequence) of the whole genome or its separate parts. (centrocardiobogota.com)
Real-time reverse transcription-polymerase chain reaction (RT-PCR), viral whole genome sequencing, and viral culture was performed on these nasal specimens. (cdc.gov)

Genomics 54, 354-5. (u-tokyo.ac.jp)
An important breakthrough in horse Y chromosome research came with the availability of new genomics tools and resources, such as large-insert genomic bacterial artificial chromosome (BAC) libraries (for review, see Rubes et al. (veteriankey.com)
It is here suggested that the word "cytogenomics" should rather be used with the goal to paraphrase a new field of research in genomics and diagnostics in human genetics, with an integrative and comprehensive view. (frontiersin.org)
Despite these discoveries, the patterns, rates, and properties of cxSVs have primarily been the focus of cancer genomics and such rearrangements remain largely underappreciated in the human germline. (biomedcentral.com)
The ﬁrst section introduces sequence-basedanalyses,includinggeneﬁnd-ing, gene family and phylogenetic analy-ses, and comparative genomics approaches. (centrocardiobogota.com)

Reducing assembly complexity of microbial genomes with single-molecule sequencing. (speakerdeck.com)
As we will describe in more detail below, irrespective of the approach, computational representations of the resulting graphs require large amounts of memory, thereby requiring substantial computational resources (both memory and run time) to assemble large genomes (such as human). (biomedcentral.com)
The 'VirION' (Viral, long-read metagenomics via MinION sequencing) approach was first validated using mock communities where it was found to be as relatively quantitative as short-read methods and provided significant improvements in recovery of viral genomes. (peerj.com)
Different variants of FISH can be used to retrieve information on genomes from (almost) base pair to whole genomic level, as besides only second and third generation sequencing approaches can do. (frontiersin.org)
In contrast to reference-based approaches which relate sequences to a single genome, these data structures encode the mutual relationships between all the genomes represented. (biorxiv.org)

Multi-dimensional pooling of 295,680 BAC clones and sequencing the ends of restriction fragments of pooled DNA generated 1,814 million high quality reads, of which 825 million were deconvolutable to 1.11 million unique WGP sequence tags. (frontiersin.org)
Assembly of the BAC clones using the modified Fingerprinted Contigs (FPC) program has resulted in 13,040 contigs, consisting of 213,719 BACs, and 6,294 singleton BACs. (frontiersin.org)
Additionally, the 1.1 million WGP tags will support efficient assignment of sequence scaffolds to the BAC clones, and thus an efficient sequencing of BAC pools with targeted genome regions of interest. (frontiersin.org)
WGP technology is based on generation of short sequence tags from terminal ends of restriction fragments of individual BAC clones, followed by assembly of BAC clones into contigs based on shared regions containing identical sequence tags. (frontiersin.org)
16 Selection for the active X chromosome yielded 12 clones bearing only the normal X and five clones retaining both X chromosomes. (bmj.com)
the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries. (jax.org)
Starting with two cosmids within 3p21.1, D3S92 and D3S93, we have isolated two separate contigs of overlapping cosmids within 3p21.1, by screening a library of 5700 chromosome 3-specific cosmid clones. (elsevierpure.com)
A total of 207 BAC clones containing 155 loci were isolated and arranged into a map of linearly ordered overlapping clones over the proximal part of horse chromosome 21 (ECA21), which corresponds to the proximal half of the short arm of human chromosome 19 (HSA19p) and part of HSA5. (uaeu.ac.ae)
The clones form two contigs - each corresponding to the respective human chromosomes - that are estimated to be separated by a gap of ∼200 kb. (uaeu.ac.ae)
1993) Isolation and FISH mapping of 80 cosmid clones on the short arm of human chromosome 3. (u-tokyo.ac.jp)

5 megabase contig on mouse chromosome 1. (u-tokyo.ac.jp)

Many different restriction fragments derived from these two contigs were found to be evolutionarily conserved and hybridized to distinct message transcripts. (elsevierpure.com)
These fragments were used to identify homologous cDNAs from an adenogastric cDNA library, and several of these cDNAs were partially sequenced. (elsevierpure.com)
Files included in this directory: chromAgp.zip - Description of how the working draft was generated from fragments at a chromosome layout level. (ucsc.edu)
contigAgp.zip - Description of how the working draft was generated from fragments at a contig layout level. (ucsc.edu)
The number of contigs drops as the fragments are joined. (uthsc.edu)
The short cDNA sequence Fragments when making this method are called expressed sequence tags , or ESTs. (multi-mania.be)
Genome assembly is the process of merging fragments of a DNA sequence produced by shotgun sequencing in order to reconstruct the original genome. (biomedcentral.com)

WARNING: The following transcripts appear on multiple chromosomes in the GTF: This can indicate a problem with the reference or annotations. (10xgenomics.com)

This sequence-based physical map will be useful to assemble the genome sequence of pea. (frontiersin.org)
WGP is designed based on the use of sequence tags generated by next generation sequencing (NGS) and is a powerful alternative to traditional DNA fingerprinting based physical mapping technologies, and also simultaneously generates a partial genome sequence. (frontiersin.org)
To create and use a custom reference package, Space Ranger requires a reference genome sequence (FASTA file) and gene annotations (GTF file). (10xgenomics.com)
Slide 3 As you can see from this figure, most of the human genome sequence has been acquired in the past 15 months, since March 1999. (uthsc.edu)
The gene encoding PvTRAMP was identified through an extensive search of the databases hosting the genome sequence of P. vivax . (biomedcentral.com)
Here we provide a case study where we explore ways to integrate QTL data and microarray data for the pig, which has only a partial genome sequence. (biomedcentral.com)
This paper provides a case study for the integration of QTL data and microarray data for a species with limited genome sequence information and annotation. (biomedcentral.com)

The most common approaches for solving this problem (Overlap-Layout-Consensus, and the de Bruijn graph) first construct a graph encoding the relationships between the sequencing reads generated during the shotgun sequencing process. (biomedcentral.com)
Slide 4 A large BAC clone, is shotgun sequenced and all the unassembled reads from this sequence set are given one accession number and placed in Genbank in the HTGS (high throughput genomic) section. (uthsc.edu)
Venter believed therein shotgun sequencing was the fastest and must effective way to get useful human genome data. (multi-mania.be)

To map the CCR7 gene in chicken chromosome, a 6 000 rads chicken-hamster radiation hybrid panel (ChickRH6) was used. (bvsalud.org)
The high resolution framework map obtained in this study has markers covering the entire chicken chromosome 5 and reveals the existence of a high number of rearrangements when compared to the human genome. (biomedcentral.com)

Mechanisms whereby chromosomes paired during meiosis may undergo unequal crossover resulting in Williams syndrome have typically been thought to result from an unequal overlap of repetitive Alu sequences flanking the region, resulting in a type of misalignment of the chromosomal regions during a crossover event. (medscape.com)
We also identify multiple cases of catastrophic chromosomal rearrangements known as chromoanagenesis, including somatic chromoanasynthesis, and extreme balanced germline chromothripsis events involving up to 65 breakpoints and 60.6 Mb across four chromosomes, further defining rare categories of extreme cxSV. (biomedcentral.com)
gene is mapped on the wheat chromosome 5B, but, neither obtaining a physical marker nor microdissec-, tion of this chromosomal region gave practical results, [56]. (centrocardiobogota.com)

To address the challenges of accurate assembly of the pea genome (∼4.2 GB of which approximately 85% is repetitive sequences), we have adopted the WGP technology for assembly of a pea BAC library. (frontiersin.org)
In the current study, we have undertaken construction of a sequence-based physical map of pea to address the challenge in the assembly of these repetitive sequences and overcome the shortcomings of traditional restriction digestion based physical maps. (frontiersin.org)
Human genome assembly solved? (speakerdeck.com)
Chaisson, Wilson, Eichler (2015) emphasize the importance of complete de novo assembly as opposed to read mapping as the primary means to understanding the full range of human genetic variation. (speakerdeck.com)
Typical memory requirements for modern assemblers range in the hundreds of giga-bytes (GB) for human genome assembly. (biomedcentral.com)
Gene organization of the pregnancy-specific glycoprotein region on human chromosome 19: assembly and analysis of a 700-kb cosmid contig spanning the region. (nih.gov)
The assembly was produced at the Baylor College of Medicine Human Genome Seuqencing Center. (ucsc.edu)
The information graphic showing the history of the human genome assembly is part of my series of designs created for the Scientific American Graphic Science page. (bcgsc.ca)
In March 2022, a flurry of publications announced the first ever complete assembly of a human genome. (bcgsc.ca)
For modeling and analysis - such as in cancer research, for example, which is what we do here - by far the most important parts of the human genome assembly are the parts that code for protein ( transcribed regions and their ORFs ), along with their adjacent regulatory sequences . (bcgsc.ca)
Finally, this RH map was used for testing the accuracy of the chicken genome assembly for chromosome 5. (biomedcentral.com)
Only two discrepancies were observed in relation to the sequence assembly recently reported for this chromosome. (biomedcentral.com)
While in the process of finishing our map, the first draft sequence assembly of the chicken genome was released (March 1st, 2004). (biomedcentral.com)
The quality of both the GGA5 RH map and of the sequence assembly was therefore checked by alignment of all the markers by BLAST searches. (biomedcentral.com)
However, in de novo assembly, only crude metrics such as contig number and size are typically used to evaluate assembly quality. (biomedcentral.com)
The assembly problem is known to be NP hard for a number of formulations [ 1 - 3 ] and is also complicated by the many types of sequencing errors, experimental biases and the volume of data that must be processed. (biomedcentral.com)
Paradoxically, the difficulties of sequence assembly have been compounded by sequencing advances in recent years collectively termed next-generation sequencing technologies. (biomedcentral.com)
Genome assembly by maximizing likelihood has been proposed previously by Myers [ 26 ] and Medvedev and Brudno [ 1 ] but their formulations are based on simplified models that do not use important parameters, especially the sequencing error. (biomedcentral.com)
Sequence and annotation data downloads are usually made available within the first week of the release of a new assembly. (fleming.gr)
For information on extracting a large set of sequences from an assembly, see Extracting sequence in batch from an assembly . (fleming.gr)
I have a lot of coordinates for an assembly and want to extract the corresponding sequences. (fleming.gr)
For example, the sequence for human assembly hg17 can be found in ftp://hgdownload.cse.ucsc.edu/goldenPath/hg17/chromosomes/ . (fleming.gr)
To construct a DAS query, combine an assembly's base URL with the sequence entry point and type specifiers available for that assembly. (fleming.gr)

Somatic Cell and Molecular Genetics , 20 (4), 255-265. (elsevierpure.com)
Cytogenetics and Cell Genetics , 85(3-4):232-6. (u-tokyo.ac.jp)
One such report appeared on January 26, 2001 on the, Genetics, Inc. regulating chromosome pairing in polyploid plants. (centrocardiobogota.com)

For a given genome coverage c (the average number of reads covering a particular base in the genome) for every read, this approach, thus, requires storing approximately c overlaps, each of which requires storing a 4-8 byte pointer, as well as at least another 2 bytes of additional information about the overlap (coordinates within the reads, level of similarity, etc. (biomedcentral.com)
Variants (SNPs and short INDELs) in sequencing data are identified mainly through mapping-based approaches [ 9 - 11 ], in which raw sequencing reads are first mapped to a reference sequence and the sites differing between reads and the reference are then identified by variant calling [ 9 , 12 ]. (biomedcentral.com)
Slide 5 Here the reads have been assembled into contigs by searching for overlaps. (uthsc.edu)
Despite the intrinsic complexity of human genome sequences, reads were easily mapped and analyzed, and all algorithms used provided the same results. (molvis.org)

In this study, the complete genomic sequence of Fusarium equiseti D25-1 was determined using multiple sequencing platforms. (researchsquare.com)

That changed when the Supreme Court decided, in Dickinson v. Zurko (1999), pursuant to the provisions of the Administrative Procedures Act (5 U.S.C. §§ 551 et seq . (patentdocs.org)

The probes have been used for molecular hybridizations (fluorescence in situ hybridization, or FISH) in clinical cytogenetics, Y chromosome mapping, sequencing, gene discovery, and comparative Y chromosome studies between the horse and other equids/ Perssodactyls. (veteriankey.com)
This review is about "molecular cytogenetics" including 1) the historical perspective of its development from cytogenetics, 2) technical aspects, 3) available probe sets, and 4) variants and applications of the basic fluorescence in situ hybridization (FISH) approach. (frontiersin.org)

Chicken is the first major agricultural species for which the complete genome sequencing was undertaken. (biomedcentral.com)

Chromosome painting, performed on metaphase chromosomes preparations with an X chromosome specific probe (P5222-DG.5, Oncor), 17 confirmed that these hybrids contained only one X chromosome. (bmj.com)
Dual color fluorescence in situ hybridization on metaphase and interphase chromosomes verified the relative order of some of the BACs and helped to orient them accurately in the contigs. (uaeu.ac.ae)

The dominant theoretical model for the early stages of sex chromosome evolution 3 , 4 , 5 predicts that recombination will be selected against in the region between a sex determining gene and a nearby locus with alleles of sex-specific effect. (nature.com)
Therefore, in this system, current models of sex chromosome evolution predict that recombination would be selected against between the sex determining locus and linked loci involved in colouration. (nature.com)
One contig extends for 100 kb and encompassed both ACY1 and D3S92, and the other extends about 80 kb around the D3S93 locus. (elsevierpure.com)
Positions Contents --------- -------- 01-05 'LOCUS' 06-12 spaces 13-28 Locus name 29-29 space 30-40 Length of sequence, right-justified 41-41 space 42-43 'bp' 44-47 spaces 48-54 DNA, RNA, mRNA, rRNA, tRNA or cRNA, left justified 55-55 space 56-63 'linear' followed by two spaces, or 'circular' 64-64 space 65-67 The division code (see '3.1. (nig.ac.jp)
We previously localized a Quantitative Trait Locus for HPFH in an extensive Asian-Indian kindred to chromosome 6q23. (biomedcentral.com)
Therefore, we reasoned that MDH1 could correspond to the RP28 locus, and mutations in its sequence could be responsible for the disease in a manner similar to that of pathogenic changes in IDH3B . (molvis.org)

This process would shrink the pseudoautosomal region in favour of expanding X- and Y-specific regions, creating a male supergene on the Y chromosome containing multiple colouration loci and thereby resolving sexually antagonistic selection. (nature.com)
Quantitative trait loci (QTL) for age of puberty, nipple number and ovulation rate have been identified in Meishan crosses on pig chromosome 10q (SSC10) near the telomere, which is homologous to human chromosome 10p15 and contains an aldo-keto reductase (AKR) gene cluster with at least six family members. (biomedcentral.com)
This file defines the order of the loci presented in per-chromosome downloadable genotype data. (ox.ac.uk)

How do I obtain the sequence and/or annotation data for a release? (fleming.gr)
You can download sequence and annotation data using our FTP server , but we recommend using rsync, which has the advantage of starting up where it left off after a failure, when run again. (fleming.gr)
The entry point specifies chromosome position, and the type indicates the annotation table requested. (fleming.gr)

To confirm the validity of the map and to provide valuable comparative mapping information, both markers from the genetic map and a high number of ESTs (Expressed Sequence Tags) were used. (biomedcentral.com)

4, 5 Until recently, the only known contiguous gene syndrome involving the COL4A5 gene was Alport syndrome and diffuse leiomyomatosis (ATS-DL, OMIM 308940), 6- 9 in which the deletion extends towards the centromere to include the first two exons of the adjacent COL4A6 gene. (bmj.com)
A long range deletion map was constructed using available X chromosome YAC contigs and the DNA of the proband of family 1, hybrid 1C3 derived from the mother of family 2, and patients 3 and 4. (bmj.com)
The extent of these four deletions was compared with a previously reported deletion present in patient 5, diagnosed with ATS-DL. (bmj.com)
In virtually all cases of Williams syndrome, haploinsufficiency (loss of 1 of 2 copies) due to a deletion at chromosome band 7q11.23 that involves the elastin gene ( ELN ) is implicated. (medscape.com)
3 : Deletion on the subject sequence. (ox.ac.uk)

Two-dimensional or multi-dimensional BAC clone pooling is an effective strategy for DNA preparation and sequencing to reduce the costs of sample preparation. (frontiersin.org)
Back selection for the inactive X on one diploid clone yielded 12 hybrids containing the deleted inactive X chromosome, three of which were selected for analysis. (bmj.com)
450 /chromosome="12" /clone="GT200015" /clone_lib="lambda gt11 human liver cDNA (GeneTech. (nig.ac.jp)
to determine the id, 1 recursivMapping Title : recursivMapping Usage : $obj->recursivMapping($newval) Function: Flag for activating the recursive mapping (desactivated by default) Returns : value of recursivMapping (a scalar) Args : on set, new value (a scalar or undef, optional) Goal : When we have a clone mapped on a chromosome, the recursive mapping maps the features of the clone on the chromosome. (perl.org)

1994 Jul;20(4):255-265. (elsevierpure.com)
1994 Oct;5(10):597-607. (u-tokyo.ac.jp)
1994) IRE-bubble PCR: a rapid method for efficient and representative amplification of human genomic DNA sequences from complex sources. (u-tokyo.ac.jp)

The resolution of radiation hybrid (RH) maps is intermediate between that of the genetic and BAC (Bacterial Artificial Chromosome) contig maps. (biomedcentral.com)

The nucleotide sequence data reported have been submitted to GenBank and assigned accession numbers [GenBank:DQ474064-DQ474068, GenBank:DQ494488-DQ494490 and GenBank:DQ487182-DQ487184]. (biomedcentral.com)
This sequence data is updated once a week via automatic GenBank updates. (ucsc.edu)
5. Any redistribution of the data should carry this notice. (ucsc.edu)
Notice for patent related sequence data 4. (nig.ac.jp)
This morning I would like to talk about data mining in the human genome and what king of problems you may run into. (uthsc.edu)
These data provide a foundational map of large SV in the morbid human genome and demonstrate a previously underappreciated abundance and diversity of cxSV that should be considered in genomic studies of human disease. (biomedcentral.com)
The available human/chicken comparative mapping data indicated conservation of synteny between GGA5 and portions of HSA11, HSA14 and HSA15. (biomedcentral.com)
means that in downloaded data for Chromosome 1, the 7th value corresponds to Affymetrix ID 14143477 which is also RS ID rs12562034, and is a True SNP wherein exactly one nucleotide on the flanking sequence is replaced with exactly one nucleotide on the subject sequence. (ox.ac.uk)
have recently demonstrated that high-quality de novo assemblies can be routinely generated from third-generation long read sequencing data. (biorxiv.org)
A. Download the appropriate fasta files from our ftp server and extract sequence data using your own tools or the tools from our source tree. (fleming.gr)
This is the recommended method when you have very large sequence datasets or will be extracting data frequently. (fleming.gr)
Sequence data for most assemblies is located in the assembly's 'chromosomes' subdirectory on the downloads server. (fleming.gr)
Select the custom track in the Table browser, then select the 'sequence' output format to retrieve data. (fleming.gr)

These WGP tags were used to assemble 220,013 BACs into contigs. (frontiersin.org)
The BACs provide a 4-5× coverage of the region and span an estimated length of ∼3.3 Mb. (uaeu.ac.ae)

In the past this has been done with the help of a number of measures such as N50 scaffold or contig lengths - which is the maximum contig (scaffold) length such that at least half the total length is contained in contigs (scaffolds) of length greater than or equal to that length. (biomedcentral.com)

Sex chromosomes evolve once recombination is halted between a homologous pair of chromosomes. (nature.com)
Sex chromosomes are typically thought to evolve as recombination is halted between a homologous pair of chromosomes in one sex. (nature.com)
One of the QTL regions is located on the long arm of pig chromosome 10 (SSC10q) near the telomere, which is homologous to human chromosome 10p15 [ 5 , 6 ]. (biomedcentral.com)

A single nucleotide on the flanking sequence is substituted with the sequence of nucleotides with total length more than one. (ox.ac.uk)
Exactly one nucleotide on the flanking sequence is replaced with exactly one nucleotide on the subject sequence. (ox.ac.uk)

92% of the human genome is euchromatic. (bcgsc.ca)
This polymorphism is primarily attributed to the various amount of Y heterochromatin, though earlier studies also suggest variation in the euchromatic portion of the chromosome (Power, 1988). (veteriankey.com)
1992). Due to the largely heterochromatic nature of ECAY and repeat-biased amplification of DOP-PCR, microdissected probes tend to be enriched with repetitive sequences, while euchromatic sequences remain underrepresented. (veteriankey.com)

We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken CCR7 gene, and found that the assembled contig shared a high percentage of similarity with that of the human gene. (bvsalud.org)

He suggested to introduce the term chromosomics being equal to cytogenomics to bring the three-dimensional morphologically of chromosomes into the focus of research, as this is essential for gene regulation. (frontiersin.org)

Here especially highlighted variations of FISH are molecular combing, chromosome orientation-FISH (CO-FISH), telomere-FISH, parental origin determination FISH (POD-FISH), FISH to resolve the nuclear architecture, multicolor-FISH (mFISH) approaches, among other applied in chromoanagenesis studies, Comet-FISH, and CRISPR-mediated FISH-applications. (frontiersin.org)
The sequences also show an The conventional and marker-assisted breeding (MAB) are the two approaches used to accomplish plant breeding (Breseghello and Coelho, 2013). (centrocardiobogota.com)
The dashed arrows outline an alternative strategy where comparisons are made on the human genome, using comparative mapping approaches. (biomedcentral.com)

chromFa.zip - The working draft sequence chromosome in one file per chromosome. (ucsc.edu)
For those of you that have looked at the human genome rough draft sequence, you have probably had some frustrations. (uthsc.edu)

2007). This allowed isolation of ECAY sequences and generation of Y-specific molecular probes. (veteriankey.com)

Mules are infertile because they inherit 32 chromosomes from their horse parent, but only 31 chromosomes from their donkey parent-and so have an odd chromosome that they cannot pair-off when they make sperm or egg cells. (elifesciences.org)
However, even if a hybrid inherits the same number of chromosomes from each parent, if the chromosomes from the two parents have different structures, the hybrid may still be infertile. (elifesciences.org)
Additionally, there are two reports about infertile stallions having XXY sex chromosomes - an equine counterpart to human Kleinfelter syndrome (Kubien et al. (veteriankey.com)

High-resolution physical mapping by combined Alu-hybridization/PCR screening: construction of a yeast artificial chromosome map covering 31 centimorgans in 3p21-p14. (u-tokyo.ac.jp)
have now looked at two species of fission yeast- S. pombe and S. kambucha -that share 99.5% of their DNA sequence. (elifesciences.org)
Although hybrids of these two species inherit three chromosomes from each parent, the majority of spores (the yeast equivalent of sperm) that these hybrids produce fail to develop into new yeast cells. (elifesciences.org)

Leukemia 2007(6): 1303-5 [ PubMed abstract ]. (lu.se)
Br J Haematol 2006(4): 448-50 [ PubMed abstract ]. (lu.se)
Blood 2005(6): 2183-5 [ PubMed abstract ]. (lu.se)
Hum Mutat 2005(5): 506 [ PubMed abstract ]. (lu.se)

The total repetitive sequence length was 1,713,918 bp, accounting for 4.2033% of the genome. (researchsquare.com)

Mutations of the PTPN11 gene in therapy-related MDS and AML with rare balanced chromosome translocations. (lu.se)
Identification of Bruton tyrosine kinase mutations in 12 Chinese patients with X-linked agammaglobulinaemia by long PCR-direct sequencing. (lu.se)

Genetic and biological characterization of tick-borne were serologically diagnosed, demonstrating that humans encephalitis virus isolated from wild rodents in southern Hokkaido, Japan in 2008. (cdc.gov)
1993) Clinical and genetic studies of renal cell carcinomas in a family with a constitutional chromosome 3;8 translocation. (u-tokyo.ac.jp)

I'll give you an example from chromosome 19 which has a CYP2 family gene cluster. (uthsc.edu)

If you had looked for a human gene in Genbank before 1998 you probably would not have found it. (uthsc.edu)

However, read lengths are considerably shorter and error rates are higher than those in Sanger sequencing. (biomedcentral.com)

In 1888, Heinrich W. Waldeyer introduced the name "stained body" as "chromosome" ( Waldeyer, 1888 ) for what Gregor Mendel already postulated as "Kopplungsgruppen", which refers to "linked up groups" in German ( Mendel, 1866 ). (frontiersin.org)

At least three distinct meiotic drive alleles, one on each S. kambucha chromosome, independently contribute to hybrid infertility by causing nonrandom spore death. (elifesciences.org)

This might be because rearrangements in the gene-poor Y chromosome have milder effect on the phenotype, as a result of which the affected individuals escape attention and are not subjected to cytogenetic analysis. (veteriankey.com)
The only true Y chromosome structural rearrangements, reported so far, are large-scale ECAY deletions in 64,XY sex reversal mares (Raudsepp et al. (veteriankey.com)

Large-scale refers to regions with size on the order of a chromosome (that is, 30 Mb or more). (ucsc.edu)
Therefore, microdissected Y-chromosome probes are good for FISH but not suitable for cloning and sequencing of the male-specific or pseudoautosomal regions of the chromosome. (veteriankey.com)
Constraints on the design of primers were to avoid presence of long introns, whose position and length was predicted on the basis of the orthologous human gene structure, and to design primers in the most divergent regions of the human/chicken alignment, to limit cross-amplification with the hamster DNA present in the hybrids. (biomedcentral.com)

results can serve as an important argument in favour of the proposal to develop a special program for sequencing the genome chromosomes and for, To estimate the possibility of plant genome mapping using human genome probes, the probes fluorescent in situ hybridization (FISH) of human 18S-28S rDNA (clon 22F9 from the LA-13NCO1 library) was carried out on chromosomes of the spring barleyHordeum vulgareL. (centrocardiobogota.com)

Repeats from RepeatMasker and Tandem Repeats Finder (with period of 12 or less) are in lower case while non-repeating sequence is in upper case. (ucsc.edu)
The full set of arguments used for the RepeatMasker operation were: -ali -s -spec rat -comp mouse chromTrf.zip - Tandem Repeats Finder locations, filtered to keep repeats with period less than or equal to 12, translated into one .bed file per chromosomes. (ucsc.edu)

Anatomy57

Organisms12

Diseases19

Chemicals and Drugs17

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes145

Disciplines and Occupations5

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture3

Information Science10

Health Care1

Genes46

Whole-genome9

Genomics5

Genomes5

Clones10

Mouse chromosome1

Fragments7

Multiple chromosomes1

Genome sequence7

Shotgun3

Chicken chromosome2

Chromosomal3

Assembly23

Genetics3

Reads4

Genomic Sequence1

19991

Cytogenetics2

Complete genome1

Metaphase2

Locus6

Loci3

Annotation3

ESTs1

Deletion5

Clone4

19943

Radiation hybrid1

Data13

BACs2

Scaffolds1

Homologous3

Nucleotide2

Euchromatic3

Similarity1

Morphologically1

Approaches3

Draft sequence2

Isolation1

Infertile3

Yeast3

Abstract4

Repetitive sequence1

Mutations2

Genetic2

Gene cluster1

GenBank1

Sanger1

Gregor Mendel1

Distinct1

Rearrangements2

Regions3

Hybridization1

Repeats2