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  • affinity
  • The binding affinity of NOX-S93 to S1P had a Kd value of 4.3 nM. (nih.gov)
  • Only starting with round seven the ratio of bound RNA compared with the applied concentration of L-e-S1P(bio) slowly increased, indicating emerging affinity in the library (Supplementary Figure S1A at http://www.biochemj.org/bj/462/bj4620153add.htm). (nih.gov)
  • upstream
  • An UP-element that can bind the C-terminal domain of the RNA polymerase (RNAP) alpha-subunit was cloned upstream of the DNA site for CRP at CC(-41.5) and downstream of the DNA site for CRP at CC(-72.5). (biochemj.org)
  • The positive effects of binding RNAP alpha-subunits upstream of the DNA site for CRP at -41.5 are suppressed if the UP-element is incorrectly positioned. (biochemj.org)
  • genome
  • strain PCC7120 has been cloned by PCR amplification with primers designed after TBLASTN analysis of its genome sequence using the Escherichia coli LexA sequence as a probe. (springer.com)
  • We developed an algorithm (Trident) to search genome-wide for potential triplex-forming sites and show that several mammalian and non-mammalian genomes are enriched for strong microRNA triplex binding sites. (osti.gov)
  • Show
  • Double-helical DNA is capable of forming triple-helical structures through Hoogsteen and reverse Hoogsteen interactions in the major groove of the duplex, and we show physical evidence that microRNAs form triple-helical structures with duplex DNA, and identify microRNA sequences that favor triplex formation. (osti.gov)
  • thus
  • The cofactor nicotinamide adenine dinucleotide disrupted complex formation between tRNA and GAPDH and thus may share a common binding site with the RNA. (sciencemag.org)
  • sites
  • A ) Sites reporting the oldest DNA and collagen sequences are from high latitude sites compared to ostrich eggshell samples from sites in Africa illustrated in ( B ) for which the current mean annual air temperatures are much higher. (elifesciences.org)
  • The resulting collection of descriptors can be used to screen sequence databases in order to predict functional sites. (utexas.edu)
  • Traditionally, these binding sites have been viewed only as docking sites. (pubmedcentralcanada.ca)
  • mechanism
  • In the case of EBV, autoregulation of BRLF1/Rta, which is a homologue of ORF50/Lyta, is also observed in EBV lytic replication, and this regulation occurs through a non-DNA-binding mechanism of Rta in certain cell lines ( 24 , 39 ). (asm.org)
  • nuclear
  • To examine the higher-order packaging of these in vivo SATB1-binding sequences, high-resolution in situ fluorescence hybridization was performed with both nuclear "halos" with distended loops and the nuclear matrix after the majority of DNA had been removed by nuclease digestion. (ovid.com)
  • In human breast cancer SK-BR-3 cells that do not express SATB1, at least one such sequence was found not anchored onto the nuclear matrix. (ovid.com)
  • replication
  • All in vivo SATB1-binding sequences examined contained typical ATC sequence contexts, with some exhibiting homology to autonomously replicating sequences from the yeast Saccharomyces cerevisiae that function as replication origins in yeast cells. (ovid.com)
  • entity
  • These are represented by 1 sequence-unique entity. (rcsb.org)
  • In the table for each entity, view a list of similar sequences by selecting the link associated with the percentage cutoff. (rcsb.org)
  • domains
  • , The functional domains of SATB2 consist of two CUT domains that aid in the binding to the matrix attachment region (MAR) and a homeodomain at the C-terminus, both of which are highly conserved through evolution and across vertebrate taxa, such as mouse, chicken, and zebrafish. (carcinogenesis.com)
  • concentration
  • In order to select for the best binding aptamers, the stringency of the selection process was gradually increased by decreasing the concentration of both target and library to a final concentration of 40 pM and 2.5 nM respectively, in round 16. (nih.gov)
  • dependent
  • We conclude that TrpC4 α is selectively involved in mechanisms downstream of PLC β 1b culminating in cardiomyocyte hypertrophy, and that the hypertrophic response is dependent on the TrpC4 α splice variant-specific sequence that binds to PIP 2 . (aspetjournals.org)
  • functional
  • Within these 15-bp GBSs, five positions are nearly invariant, whereas the remainder can be altered with little effect on GR binding ( 4 ) and vary substantially among all functional GBSs ( 5 ). (pubmedcentralcanada.ca)
  • shows
  • The graphical representation below shows this entry's sequences as reported in UniProtKB, in the sample (SEQRES), or as observed in the experiment (ATOM). (rcsb.org)
  • The Spiegelmer® shows equal binding to dihydro-S1P, but no cross-reactivity to the related lipids sphingosine, lysophosphatidic acid, ceramide, ceramide-1-phosphate or sphingosine phosphocholine. (nih.gov)
  • expression
  • Cotransfection of ORF50/Lyta with an Oct-1 expression vector enhanced the transactivation mediated by the Oct-1-binding element. (asm.org)
  • factor
  • Our results suggest a new physiological role for hnRNP D0B that involves binding to double- and single-stranded DNA sequences in a specific manner and functioning as a transcription factor. (biochemj.org)
  • contains
  • If a derived type is declared containing a UNION, and the type is a SEQUENCE type or is interoperable (BIND(C)), and the UNION contains a misaligned field, the compiler incorrectly adds padding bytes before each misaligned field. (chiplist.com)