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  • single-stranded
  • DNA and RNA, such as obtained by denaturing their double stranded forms, will hybridize or recombine under appropriate conditions with complementary single stranded nucleic acids. (google.ca)
  • By labeling such complementary probe nucleic acids with some readily detectable chemical group, it was then made possible to detect the presence of any polynucleotide sequence of interest in a test medium containing sample nucleic acids in single stranded form. (google.ca)
  • function
  • It includes sophisticated editing options and provides a range of analysis tools to investigate the structure and function of macromolecules through a multiple window interface. (jalview.org)
  • technique
  • In addition to the recombinant DNA field, the analytical hybridization technique can be applied to the detection of polynucleotides of importance in the fields of human and veterinary medicine, agriculture, and food science, among others. (google.ca)
  • As the technology for ancestral sequence reconstruction has advanced the technique has popularized. (openwetware.org)
  • primers
  • Phylogeny of R. fascians isolates obtained from Plant Clinic submissions was determined with 16S ribosomal DNA (16S rDNA) sequence analysis with sequence primers designed to amplify specific regions of R. fascians 16S rDNA. (oregonstate.edu)
  • They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes. (wikipedia.org)
  • 2001
  • 2001) showing near total congruence of mtDNA-based and nuclear-based trees when such sequences were excluded, some authors continued to produce misleading trees (Arnason et al. (wikipedia.org)
  • 2001). "Prediction of the coding sequences of unidentified human genes. (wikipedia.org)
  • novo
  • As the amount of ctDNA in cfDNA varies (and is often low in patients with low tumor burden), de novo discovery of mutations by plasma DNA analysis is not trivial. (ascopost.com)
  • Whether sequencing of ctDNA will render tumor biopsies redundant remains to be determined, particularly because of the strong association between ctDNA yield and disease burden and the technologic and bioinformatic developments required for de novo discovery of mutations based solely on ctDNA analysis. (ascopost.com)
  • tumor
  • Also discussed are important recent diagnostic applications of DNA sequencing in cancer, including analysis of tumor derived cell free DNA and exosomes that are present in body fluids. (frontiersin.org)
  • Sequencing Analysis of Tumor DNA: Is It All in the Plasma? (ascopost.com)
  • Circulating tumor DNA (ctDNA) in plasma comprises a fraction of cfDNA, and is believed to be shed in the bloodstream by cancer cells through apoptosis, necroptosis, or secretion (eg, exosomes) not only from the primary tumor, but also metastatic lesions and minimal residual disease. (ascopost.com)
  • In fact, there is evidence to suggest that these techniques may be useful for disease monitoring in cases where the primary tumor has been previously subjected to sequencing or for patients with tumor types characterized by the presence of highly recurrent mutations, as well as for the identification of emerging mutations already known to result in therapy resistance. (ascopost.com)
  • Synthesizer
  • Caruthers and Leroy Hood at the California Institute of Technology constructed the first automated DNA Synthesizer which the MBCF successfully tested after discussions with Don Taylor. (wikipedia.org)
  • Molecular
  • Molecular analysis of the patient isolate allowed the classification of the strain to B. lusitaniae, a genospecies previously thought to be nonpathogenic in humans The woman had a weak serological response, which is present in a high percentage of patients with unspecific and long-lasting skin manifestations. (wikipedia.org)
  • BPC) The Molecular Biology Core Facilities has had a continuous web presence since 1992 and began receiving primer sequence for high throughput DNA synthesis by web forms in 1995 when new high volume DNA synthesizers were brought on-line. (wikipedia.org)
  • synthetic
  • Typically, synthetic oligonucleotides are single-stranded DNA or RNA molecules around 15-25 bases in length. (wikipedia.org)
  • microarray
  • And, for those experiments where you are looking for concordance between your RNA-Seq and Microarray data, shouldn't you have the concordance in your analysis software as well? (genecodes.com)
  • computational
  • By consequence, many computational biologists have intensified studies in order to understand the content of these sequences and, in some cases, to search for association to disease. (springer.com)
  • synthesis
  • It appears that most spontaneously arising mutations result from error prone replication (trans-lesion synthesis) past a DNA damage in the template strand. (wikipedia.org)
  • detection
  • The sequencing techniques currently available for the detection of known or "hotspot" mutations, however, offer incredibly high levels of sensitivity. (ascopost.com)
  • next-gen
  • An Illumina MiSeq was evaluated on multiple projects and now a series of three MiSeqs have been streamlined for fast analysis of Next Gen samples. (wikipedia.org)
  • human
  • In addition, the Human Genome Project facilitated the development of technologies for high throughput sequencing of DNA and enhanced methods for DNA sequence analysis. (frontiersin.org)
  • Capabilities for characterizing human DNA sequence variations, including sequence variations that led to human diseases were thus facilitated. (frontiersin.org)
  • The results are discussed in light of what is known about the preservation of microbial DNA at the Iceman's site and of previous parasitological studies performed on the Iceman himself and on human coprolites. (calpoly.edu)
  • Our finding that the Neanderthal and human genomes are at least 99.5% identical led us to develop and successfully implement a targeted method for recovering specific ancient DNA sequences from metagenomic libraries. (sciencemag.org)
  • The automated DNA sequencer was an enabling technology for the Human Genome Project. (wikipedia.org)
  • mutations
  • DNA sequence information gathered from healthy and diseased tissues is highly valuable in the medical field as mutations can be identified, individualized medical care can be administered, and organisms identified and cataloged. (wikipedia.org)
  • However, some DNA damages remain and give rise to mutations. (wikipedia.org)
  • Thus it seems that DNA damages are the underlying cause of most spontaneous mutations, either because of error-prone replication past damages or error-prone repair of damages. (wikipedia.org)
  • multiple
  • Despite multiple papers pointing out that some mitochondrial sequences showed unusual properties (particularly murid rodents and hedgehogs) and were likely distorting the overall tree (Sullivan and Swofford 1997, Waddell et al. (wikipedia.org)
  • cell free
  • Although the presence of circulating DNA in plasma has long been known, circulating cell-free plasma DNA (cfDNA) is detected in normal individuals, and its measure is of limited utility in oncology. (ascopost.com)
  • initial analysis
  • This initial analysis of the Neanderthal genome advances our understanding of the evolutionary relationship of Homo sapiens and Homo neanderthalensis and signifies the dawn of Neanderthal genomics. (sciencemag.org)