• Massively parallel sequencing analyses have demonstrated that most of the common malignancies display relatively complex repertoires of somatic genetic alterations, that the number of highly recurrent mutations is limited, and that a large number of genes is mutated in a small minority of tumors from a given cancer type. (ascopost.com)
  • Evidence suggests that massively parallel sequencing analysis of ctDNA from patients with advanced disease allows for the identification of the entire constellation of somatic mutations found in cancer cells, either from the primary tumor or from metastatic lesions. (ascopost.com)
  • Recent advances in DNA sequencing methodologies have caused an exponential growth of publicly available genomic sequence data. (springer.com)
  • Several lines of evidence indicate that the 65,250 base pairs of hominid sequence so far identified in the library are of Neanderthal origin, the strongest being the ascertainment of sequence identities between Neanderthal and chimpanzee at sites where the human genomic sequence is different. (sciencemag.org)
  • As the amount of ctDNA in cfDNA varies (and is often low in patients with low tumor burden), de novo discovery of mutations by plasma DNA analysis is not trivial. (ascopost.com)
  • Whether sequencing of ctDNA will render tumor biopsies redundant remains to be determined, particularly because of the strong association between ctDNA yield and disease burden and the technologic and bioinformatic developments required for de novo discovery of mutations based solely on ctDNA analysis. (ascopost.com)
  • By consequence, many computational biologists have intensified studies in order to understand the content of these sequences and, in some cases, to search for association to disease. (springer.com)
  • Yes, deletions hide information (e.g., sequence substitutions or other smaller deletions which were contained within the larger deleted region) and may be the result of parallel evolutionary changes (stem-loop structures will have a common underlying _mechanism_ for their deletion with the pattern of removal being independent of the lineage relationships). (bio.net)
  • 12. Highlight the entire coding sequence, including the start and stop codons (375 bases in the wild-type with no insertions or deletions). (navinpokala.org)
  • A Protein Sequencer using Edman degradation was installed in 1989 quickly followed by several DNA Sequencers which were the first to use fluorescent dye terminator chemistry. (wikipedia.org)
  • A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer. (nih.gov)
  • An Illumina MiSeq was evaluated on multiple projects and now a series of three MiSeqs have been streamlined for fast analysis of Next Gen samples. (wikipedia.org)
  • With powerful analyses and rich visualizations, it has a unique paradigm where every analysis is an experiment that make your work really flow. (genecodes.com)
  • Examples of cases where exome sequencing has facilitated diagnosis and led to improved medical management are presented. (frontiersin.org)
  • Two broad areas that Huston's research focuses are developing computer models for cellular networks and performing DNA analysis. (txstate.edu)
  • Our finding that the Neanderthal and human genomes are at least 99.5% identical led us to develop and successfully implement a targeted method for recovering specific ancient DNA sequences from metagenomic libraries. (sciencemag.org)
  • Caruthers and Leroy Hood at the California Institute of Technology constructed the first automated DNA Synthesizer which the MBCF successfully tested after discussions with Don Taylor. (wikipedia.org)
  • Although the presence of circulating DNA in plasma has long been known, circulating cell-free plasma DNA (cfDNA) is detected in normal individuals, and its measure is of limited utility in oncology. (ascopost.com)