• The DNA sequences of ospA , ospB , rrs-rrlA , and ospC ideal for deer and the small mammals that maintain the I. were determined for use in our phylogenetic analysis. (cdc.gov)
  • We will utilize several computational strategies to identify the genome difference between Lso haplotypes, including whole genome comparisons, ortholog analysis, gene neighborhood analysis, phylogenetic inference and conservation analysis. (usda.gov)
  • Sequencing results will be further incorporated into comparative genomics and phylogenetic analyses (approaches in Objective 1) to correlate genotypic data with our phenotypic knowledge. (usda.gov)
  • There is also the phenomenon of HAP2 - a fusion protein that is used by many different eucaryotic organisms "all over" the phylogenetic tree (from Chlamydomonas to Bees) for mediating cell-cell fusion, predominantley of gametes. (virology.ws)
  • Different computational methods have been designed for estimating protein function based on the information generated from sequence similarity, subcellular localization, phylogenetic profiles, mRNA expression profiles, homology modelling etc. [ 15 ]. (biomedcentral.com)
  • Kirstyn Brunker completed a Ph.D. at the University of Glasgow in 2016, where she established whole genome sequencing and phylogenetic pipelines to study rabies virus transmission in Tanzania. (nanoporetech.com)
  • Phylogenetic analysis with DNA amplification technologies. (who.int)
  • Several techniques based on the polymerase chain reaction (PCR) to facilitate the characterization and phylogenetic analysis of variola virus isolates were described. (who.int)
  • There is little information on their evolution in Jordan so this study investigated the genetic and antigenic variability of A(H1N1)pdm09 and H3N2 viruses in Jordan by performing phylogenetic and genetic analyses of the HA and NA genes of A(H1N1)pdm09 and H3N2 viruses between 2011 and 2013. (who.int)
  • in Jordan, we performed a phylogenetic analyser (Applied Biosystems, Foster analysis and genetic analysis of the HA City, CA, USA). (who.int)
  • Through a whole-exome sequencing-based analysis of rare CNVs in 98 high-risk Northern Finnish breast cancer cases, the researchers aimed to tease out the role in breast cancer risk. (genomeweb.com)
  • Whole exome sequencing identifies MRVI1 as a susceptibility gene for moyamoya syndrome in neurofibromatosis type 1. (cancerindex.org)
  • Whole exome sequencing was carried out on an Italian family with moyamoya-complicated neurofibromatosis type 1 to identify putative genetic modifiers independent of the NF1 locus and potentially involved in moyamoya pathogenesis. (cancerindex.org)
  • Survival Motor Neuron Gene Copy Number Analysis by Exome Sequencing: Assisting Spinal Muscular Atrophy Diagnosis and Carrier Screening. (harvard.edu)
  • Throughout her life, she has received a number of medical opinions but it wasn't until she was in her forties, that she received a definitive diagnosis using exome sequencing that confirmed that a particular gene mutation was responsible for her condition. (cdc.gov)
  • 80%) and biological pathways (such as adipocytokine, Notch, Hedgehog and NOD-like receptor signaling) were not identified by previous gene array studies. (mdpi.com)
  • These genes each encode proteins involved in pathways previously found essential for breast cancer development. (genomeweb.com)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Further examination demonstrated that the genes exhibiting differential expression were predominantly associated with the advancement of photosynthesis, sucrose and starch metabolism, nitrogen metabolism, chloroplast development, and phenylpropanoid biosynthetic pathways, and the key potential genes were screened. (bvsalud.org)
  • Here we show that the AN gene encodes a C-terminal binding proteins/brefeldin A ADP-ribosylated substrates (CtBP/BARS) related protein. (nih.gov)
  • The M protein gene ( emm) encodes the cell surface M virulence protein responsible for at least 100 Streptococcus pyogenes M serotypes. (cdc.gov)
  • it encodes a 102 amino acid protein showing 79% similarity to TrV1. (gc.ca)
  • This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. (cancerindex.org)
  • This gene encodes a protein containing a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions. (cancerindex.org)
  • The MTHFD1L gene encodes the mitochondrial monofunctional enzyme with proven 10-formyltetrahydrofolate synthetase activity. (dcu.ie)
  • Moreover, by resolving allelic and locus heterogeneity, structural variants improve the power to identify genetic factors underlying agronomically important traits leading to, for example, the identification of two new genes potentially contributing to soluble solid content. (nature.com)
  • This method, and some of its variants, has been successfully applied to gene expression, sequence analysis, functional characterization of genes and text mining. (sciweavers.org)
  • Three transcript variants encoding different isoforms have been found for this gene. (cancerindex.org)
  • The majority of pathogenic variants are identified by DNA analysis. (jax.org)
  • For example, 11% of VUS in hereditary cancer genes are splice site variants (Karam 2019). (jax.org)
  • Landrith (2020) demonstrated in increase of 9.1% in the detection of pathogenic variants in 18 hereditary cancer genes. (jax.org)
  • UMOD risk variants identified in the above-mentioned GWAS are located in the promoter region of the gene, leading to a theory that they altered UMOD expression. (medscape.com)
  • This means taking different types of cancer, for example, as the starting point and trying to identify the genes and the different protein variants that differ between healthy and ill individuals. (lu.se)
  • To investigate further the potential role of mutated protein kinases in the development of TGCT and to characterize the prevalence and patterns of point mutations in these tumors, we have sequenced the coding exons and splice junctions of the annotated protein kinase family of 518 genes in a series of seven seminomas and six nonseminomas. (ox.ac.uk)
  • Sequencing of STK10 in an additional 40 TGCTs revealed no further mutations. (ox.ac.uk)
  • Mutations found in methyl CpG binding protein 2(MECP2) are associated with Rett syndrome. (cms.gov)
  • Better understanding of new mutations and the wide range of possible phenotypes led to the development of a new nomenclature proposal, based on the gene and inheritance pattern. (medscape.com)
  • [ 6 ] However, the gene mutations responsible for the different forms of CMT1 are clearly myelin genes. (medscape.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Increased curiosity about CAPN3 was highly stimulated when it had been reported that mutations in its gene bring about limb-girdle muscular dystrophy (LGMD) type 2A, seen as a the continuous atrophy of hip and make muscle tissues (LGMD2A, or calpainopathy) (21). (exposed-skin-care.net)
  • As opposed to other styles of muscles dystrophy, where mutations take place in genes encoding structural protein, calpainopathy was the initial reported kind of dystrophy predetermined by mutations within a gene encoding a proteolytic enzyme. (exposed-skin-care.net)
  • You can find over 440 noted mutations within the calpain 3 gene up to now, included in this 212 (50%) are missense mutations, a lot of which alter its catalytic activity (22). (exposed-skin-care.net)
  • Furthermore, the deleterious aftereffect of missense mutations can occur not merely from immediate disruption of CAPN3 function(s), but additionally from disruption from the protein's structural integrity, that could affect its intra or intermolecular protein lead and interactions to decreased stability or altered localization. (exposed-skin-care.net)
  • Complete genomic sequences of 12 different coronavirus strains demonstrated that the viral structure, with small irrelevant mutations, was stable in the transmission chains and showed remarkable traits of infectious traceability. (ersjournals.com)
  • Mutations in the gene coding for survival of motor neuron 1 protein may result in SPINAL MUSCULAR ATROPHIES OF CHILDHOOD. (harvard.edu)
  • Mutations can occur in any area of the gene. (medscape.com)
  • Murine gene mutations in human counterparts may be associated with a clinical illness different from the illness seen in mice. (medscape.com)
  • At least 17 DPY19L2 gene mutations have been found in men with globozoospermia, a condition characterized by abnormal sperm cells that have a round head and no acrosome. (medlineplus.gov)
  • Most of these mutations delete large regions of the gene or the whole gene. (medlineplus.gov)
  • These mutations lead to a loss of functional DPY19L2 protein. (medlineplus.gov)
  • MLPA and sequence analysis of DPY19L2 reveals point mutations causing globozoospermia. (medlineplus.gov)
  • Peu de renseignements sont disponibles sur les mutations des virus saisonniers de la grippe A(H1N1)pdm09 et H3N2 en Jordanie. (who.int)
  • Les mutations individuelles sont décrites en détail. (who.int)
  • This means that ultimately the genes also determines the risk for an individual to develop different disease like cancer, since genetic abnormalities, such as mutations, can lead to cancer. (lu.se)
  • There were 3954 correlations and a total of 15 correlations for differentially expressed proteins (DEPs) and differentially expressed genes (DEGs) from proteomics and transcriptomics. (hindawi.com)
  • PCC 6803 inferred from proteomics and multivariate sequence analyses. (harvard.edu)
  • Proteomics is a new science direction that explores biological activity related to protein expression [ 28 ]. (hindawi.com)
  • Within the research field of proteomics, researchers study the link between proteins and disease. (lu.se)
  • However, mapping all our proteins, which is the initial goal of the new research field known as proteomics, will take a long time. (lu.se)
  • Raf-1 kinase inhibitor protein: structure, function, regulation of cell signaling, and pivotal role in apoptosis. (wikipedia.org)
  • We now confirm those findings, as complementation of a csrS mutant restored Mg(2+)-dependent gene regulation. (lu.se)
  • The effect of Mg(2+) on regulation of global gene expression was evaluated using genomic microarrays in an M-type 3 strain of GAS and in an isogenic csrS mutant. (lu.se)
  • We observed CsrS-dependent regulation of 72 of the 73 genes whose expression changed in response to elevated extracellular Mg(2+) in wild-type bacteria, a result that identifies CsrS as the principal, if not exclusive, sensor for extracellular Mg(2+) in GAS. (lu.se)
  • To our knowledge, this study is the first to characterize global gene regulation by a GAS two-component system in response to a specific environmental stimulus. (lu.se)
  • When comparing the transcriptomes across developmental stages, we found an over-representation of genes involved in growth regulation in the early development stage in C. virgata . (frontiersin.org)
  • While many proteins often interact with other proteins towards expediting their functions, there are challenges that are not just limited to their function but also to their regulation [ 7 ]. (biomedcentral.com)
  • Transcriptomics focuses on gene transcription and regulation of transcription in cells [ 25 , 26 ]. (hindawi.com)
  • Molecular mechanisms for regulation of gene expression at different levels: remodeling of chromatin, initiation of transcription, nuclear transport and signalling, and RNA interference. (lu.se)
  • Principles for regulation of gene expression through intercellular signalling. (lu.se)
  • Gene regulation in developmental biology and the cell cycle. (lu.se)
  • Successful cloning and expression of the 70-kDa protein-encoding gene of phage I3 in Escherichia coli and its complete nucleotide sequence determination have been accomplished. (ias.ac.in)
  • The main tool used in transcriptomics is total RNA sequencing, which is a powerful tool for analyzing gene expression changes in response to various environmental stresses [ 27 ]. (hindawi.com)
  • To analyze the regulatory mechanism involved in the high growth potential of C. virgata , we performed RNA-seq-based transcriptome analysis and illustrated a comprehensive gene expression map of the species. (frontiersin.org)
  • Through de novo transcriptome assembly with the RNA-seq reads from whole organ samples of C. virgata at the germination stage (2 days after germination, DAG), early young development stage (8 DAG), young development stage (17 DAG), and adult development stage (28 DAG), we identified 21,589 unified transcripts (contigs) and found that 19,346 and 18,156 protein-coding transcripts were homologous to those in rice and Arabidopsis , respectively. (frontiersin.org)
  • Comprehensive transcriptome information will provide a useful resource for gene discovery and facilitate a deeper understanding of the diversity of the regulatory systems that have evolved in C. virgata while adapting to severe environmental conditions. (frontiersin.org)
  • Transcriptome sequencing of tumor subpopulations reveals a spectrum of therapeutic options for squamous cell lung cancer. (ca.gov)
  • In so doing, it demonstrates how transcriptome sequencing provides insights into the molecular underpinnings of cancer propagating cells that, importantly, can be leveraged to identify new potential therapeutic options for cancers beyond what is possible with DNA sequencing. (ca.gov)
  • METHODS: We sorted a SCC into CD133- and CD133+ subpopulations and then examined both by copy number analysis (CNA) and whole genome and transcriptome sequencing. (ca.gov)
  • Gopinathan, Karumathil P. (1994) Structural proteins of mycobacteriophage I3: cloning, expression and sequence analysis of a gene encoding a 70-kDa structural protein Gene, 143 (1). (ias.ac.in)
  • The structural proteins of mycobacteriophage 13 have been analysed by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE), radioiodination and immunoblotting. (ias.ac.in)
  • Based on their abundance the 34-and 70-kDa bands appeared to represent the major structural proteins. (ias.ac.in)
  • We will also utilize the domain-centric protein sequence and structural analyses to understand the potential functions of the identified genes. (usda.gov)
  • RNA testing looks for changes in gene expression, and protein analysis assesses structural changes in the protein product of the gene. (jax.org)
  • We have previously discussed the idea that viruses originated from selfish genetic elements such as plasmids and transposons when these nucleic acids acquired structural proteins (see A plasmid on the road to becoming a virus ). (virology.ws)
  • I want to explore in more detail the idea that the structural proteins of viruses likely originated from cell proteins ( link to paper ). (virology.ws)
  • Missing from these hypothesis is how nucleic acids became virus particles - that is, how they acquired structural proteins. (virology.ws)
  • It seems likely that viral structural proteins originated from cellular genes. (virology.ws)
  • The core proteins of alphaviruses (think Semliki Forest virus) has structural similarity with chymotrypsin-like serine proteases. (virology.ws)
  • Retroviral structural proteins also appear to have originated from cell proteins, with clear homologies with matrix, capsid, and nucleocapsid proteins. (virology.ws)
  • At some point these genetic elements acquired structural proteins from the cells and became bona fide virus particles. (virology.ws)
  • nevertheless, recent studies have got exposed a fresh potential function for CAPN3 being a structural proteins (23,24). (exposed-skin-care.net)
  • Based on the genome sequencing results, an analysis of structural proteins and prediction of putative microRNAs of Hz-2V was performed. (umass.edu)
  • Four of structural genes, p11.7, p15.1, p28.4, and p31.7 were identified by matrix assisted laser desorption/ionization time-of-flight mass spectroscopy. (umass.edu)
  • Interstingly, Hz-2V showed 93.5% of sequence homology with Hz-1V and with other nudiviruses, hytrosaviruses, and polydnaviruses. (umass.edu)
  • This graph pangenome was used for genome-wide association study analyses and heritability estimation of 20,323 gene-expression and metabolite traits. (nature.com)
  • Our results show a remarkably low mutation frequency, with only a single somatic point mutation, a K277E mutation in the STK10 gene, being identified in a total of more than 15 megabases of sequence analyzed. (ox.ac.uk)
  • Using whole-genome sequencing on drug-exposed cell lines, researchers saw high mutation rates in control cell lines intermittently treated with a compound called CX-5461. (genomeweb.com)
  • DNA testing did not identify a pathogenic variant (mutation) using a multi-gene neurology panel. (jax.org)
  • This disease, previously referred to as MCKD type 1, is due to a mutation in the variable-number tandem repeat region of the MUC1 (Mucin 1) gene. (medscape.com)
  • The frameshift mutation results in the formation of a truncated protein which cannot fold properly, promoting aggregation, and subsequent deposition in tubular cells. (medscape.com)
  • The best-aligned sequences were annotated with gene ontology groups. (frontiersin.org)
  • Beside of the trees, other data like alignments of the sequences, statistics on nucleotide changes, domain and ontology data are also available. (lu.se)
  • trees with species from the same taxon group or genes with similar ontology terms etc. (lu.se)
  • Protein analyses examine the primary structure (amino acids) as well as the secondary folded structure, more complex tertiary structures, and protein interactions. (jax.org)
  • The BTK gene is large and consists of 19 exons that encode the 659 amino acids that form the Btk cytosolic tyrosine kinase. (medscape.com)
  • Others change single protein building blocks (amino acids) in the DPY19L2 protein. (medlineplus.gov)
  • Proteins are formed of only 20 different amino acids, but almost all proteins are composed in such a way that part of the sequence is unique. (lu.se)
  • Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)
  • Southern and Northern analyses, using DNA and RNA extracted from infected Cf-124T cells, revealed the presence of both TrV1- and TrV4-carrying genome segments and transcripts, but neither DNA, at least in episomal form, nor mRNA persisted for more than a few days p.i. (gc.ca)
  • Genes contain both coding (exon) and non-coding (introns) sections that are transcribed into mRNA. (jax.org)
  • was subsequently clearly documented by means of genetically manipulated mice that generated a bicistronic mRNA encoding the OR as well as a marker protein. (jneurosci.org)
  • Biodistribution of onasemnogene abeparvovec DNA, mRNA and SMN protein in human tissue. (harvard.edu)
  • MicroRNA-9 and 197 were proven to downregulate MTHFD1L at mRNA and protein level. (dcu.ie)
  • The second part of the project will be computer based as we want to correlate the experimental results with the sequenced genomes. (dal.ca)
  • Here, the student will need to identify genes in the genomes that could be potentially involved in hydrocarbon degradation. (dal.ca)
  • In addition, over the past 4 years, the NIH funded Centers for Mendelian Genomics have conducted sequencing and analysis of protein-coding portions of more than 20,000 human genomes and have identified over 740 genes that likely cause genetic diseases. (cdc.gov)
  • Philogenetic trees has been generated for all the 893 gene groups together with the available orthologs from eukaryota genomes. (lu.se)
  • Researchers tracked down a handful of previously unappreciated susceptibility genes using exome sequences for more than 244,000 European women with or without breast cancer. (genomeweb.com)
  • This gene is a susceptibility gene for Moyamoya disease, a vascular disorder of intracranial arteries. (cancerindex.org)
  • We report on the cloning and sequencing of two Tranosema rostrale ichnovirus (TrIV) genes, and assess their relatedness to TrV1, the gene encoding the most abundant TrIV transcript in last-instar Choristoneura fumiferana larvae parasitized by T. rostrale . (gc.ca)
  • Twenty putative pre-microRNA sequences were predicted in Hz-2V by searching stem-loop structures with less than -25kcal/mol of free energy, and one of the microRNA candidates was predicted to target the Hz2V007 gene transcript, which codes for a putative juvenile hormone esterase. (umass.edu)
  • Investigators used RNAi screening and CRISPR editing validation testing in fruit flies to untangle functional contributions for conserved proteins with unknown functions. (genomeweb.com)
  • The conformational analysis was performed utilizing FT-IR matrix isolation technique and theoretical methods such as density functional theory and ab initio calculations. (researchgate.net)
  • However, for a discrete number of patients whose results are uninformative, it may be possible to get a more definitive answer about the consequences of a variant using additional methods like RNA sequencing, protein analysis and predictive or functional studies. (jax.org)
  • The predicted effects of a variant on functional areas of the protein such as binding sites for DNA or other proteins can also be analyzed. (jax.org)
  • Functional analyses use stem cells and other biological systems in vitro . (jax.org)
  • Techniques to decipher sequence-structure-function relationship, especially in terms of functional modelling of the HPs have been developed by researchers, but using the features as classifiers for HPs has not been attempted. (biomedcentral.com)
  • These known-unknown regions for which no functional links are discovered, i.e. those with no biochemical properties or obvious relatives in protein and nucleic acid databases are known as orphan genes, and the end products are called HPs [ 2 ]. (biomedcentral.com)
  • These proteins are of great importance, as many of them might be associated with human diseases, thus falling into functional families. (biomedcentral.com)
  • The question whether OR proteins are indeed present in the axons and nerve terminals of OSNs is thus of fundamental importance toward an understanding of the functional wiring in the olfactory system. (jneurosci.org)
  • Conserved motif analysis and functional predictions unveiled unique motifs in each BSM, while BSAP appeared to play a crucial role in BSM folding and pathogen resistance. (bvsalud.org)
  • There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. (nhbs.com)
  • It is amazing that now we can have this level of insight into the lifestyle of a long-extinct organism, just from doing laboratory experiments on one protein. (utoronto.ca)
  • Those RNA nucleic acid sequences are taken up by other cells in the organism and act as signalling molecules. (virology.ws)
  • Hypothetical proteins [HP] are those that are predicted to be expressed in an organism, but no evidence of their existence is known. (biomedcentral.com)
  • Genomics is the study of all genes in a cell or organism and has during the recent ten-year period been subject to an unprecedented development. (lu.se)
  • Genes determines what proteins and other building blocks an organism will consist of. (lu.se)
  • To characterize genetic diversity and gene flow among Daphnia galeata populations, the complete nucleotide (nt) sequences of the mitochondrial (mt) DNAs of D. galeata clones isolated from five lakes in Japan (Lakes Shirakaba, Suwa, Kizaki, Kasumigaura, and Biwa) were determined. (unboundmedicine.com)
  • Sequence analysis was performed to discover and characterize MTHFD1 and MTHFD1L similar sequences. (dcu.ie)
  • In this study, we conducted transcriptomic and proteomic profiling to characterize the brochosome protein composition in the rice green leafhopper Nephotettix cincticeps. (bvsalud.org)
  • This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. (cancerindex.org)
  • This gene is also a translocation partner in anaplastic large cell lymphoma and inflammatory myofibroblastic tumor cases, where a t(2;17)(p23;q25) translocation has been identified with the anaplastic lymphoma kinase (ALK) gene on chromosome 2, and a t(8;17)(q24;q25) translocation has been identified with the MYC gene on chromosome 8. (cancerindex.org)
  • In humans the protein is encoded by a single gene found near the inversion telomere of a large inverted region of CHROMOSOME 5. (harvard.edu)
  • The locus at chromosome 1q21 was identified by linkage mapping in 1998, but the gene has only recently been discovered due to difficulty with sequencing this highly repetitive region and was previously missed using next-generation sequencing. (medscape.com)
  • Since then, she has continued work on viral genomics as a postdoc in Glasgow, establishing local genomic surveillance platforms using nanopore sequencing in Tanzania and sites in Southeast Asia and Latin America. (nanoporetech.com)
  • Presently, systematic and prospective analyses of breast cancer are ongoing to evaluate genomics in blood-based monitoring of residual disease of treatment effect. (lu.se)
  • The in-silico part of the project will teach the student to work with NCBI, blast, and a variety of gene and protein sequence analysis server. (dal.ca)
  • Nucleotide sequences were initially checked using the Basic Local Alignment Search Tool hosted by the National Center for Biotechnology Information ( http://blast.ncbi.nlm.nih.gov/Blast.cgim ) for comparison with other known nucleotide sequences. (who.int)
  • This helps to avoid confounding of the 180-base M protein gene segment by the similar emm- like mrp and enn sequences (see CDC Streptococcus Laboratory GAS bioinformatic pipeline for S. pyogenes external icon ). (cdc.gov)
  • Bioinformatic analyses of DNA- and protein sequences. (lu.se)
  • With the rise in number of annotation strategies, next-generation sequencing methods have provided further understanding the functions of HPs. (biomedcentral.com)
  • While high-throughput experimental methods like the yeast two-hybrid (Y2H) method and mass spectrometry are available to discern the function of proteins, the datasets generated by these methods tend to be incomplete and generate false positives [ 9 ]. (biomedcentral.com)
  • Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. (nhbs.com)
  • A secondary goal that should be sought by a bioinformatics facility is to make algorithms and analysis methods accessible for non-computational biologists. (lu.se)
  • The students will have opportunity to practice several of these methods during laboratory work, including site-directed mutagenesis, recombinant protein production and purification, real-time RT-PCR. (lu.se)
  • Methods for gene identification and analysis of gene structure: cloning, PCR, restriction mapping, in situ hybridisation, DNA sequencing. (lu.se)
  • Methods for analysis of gene expression: microarray, hybridisation, promoter analyses. (lu.se)
  • Gene technology: basic and applied molecular genetic methods. (lu.se)
  • Sequence analysis of the protein kinase gene family in human testicular germ-cell tumors of adolescents and adults. (ox.ac.uk)
  • The protein kinase gene family is the most frequently mutated in human cancer. (ox.ac.uk)
  • Comparative genomic hybridization and LOH analysis using SNP arrays demonstrated that the 13 TGCTs mutationally screened through the 518 protein kinase genes were uniformly aneuploid with consistent chromosomal gains on 12p, 8q, 7, and X and losses on 13q, 18q, 11q, and 4q. (ox.ac.uk)
  • Our results do not provide evidence for a mutated protein kinase implicated in the development of TGCT other than KIT. (ox.ac.uk)
  • This disorder is now formally referred to as X-linked agammaglobulinemia (XLA), and the gene defect has been mapped to the gene that codes for Bruton tyrosine kinase (Btk) at band Xq21.3. (medscape.com)
  • Its activation is tightly controlled by numerous other signaling proteins including protein kinase C (PKC), Sab/SH3BP, and caveolin-1. (medscape.com)
  • We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
  • In our previous work, we developed a six-point classification scoring schema with annotation pertaining to protein family scores, orthology, protein interaction/association studies, bidirectional best BLAST hits, sorting signals, known databases and visualizers which were used to validate protein interactions. (biomedcentral.com)
  • It is a comprehensive science focusing on translational changes, posttranslational modifications, and interactions among protein molecules. (hindawi.com)
  • Comparative analysis of other Hemiptera species demonstrated that all BSM and some BSAP are specific to the Cicadellidae family. (bvsalud.org)
  • and protein sequence, structure, and interaction analysis. (mdpi.com)
  • This is not surprising given the strong evidence for interaction between myelin and axon gene expression in development and after experimental nerve lesions. (medscape.com)
  • One such widely used technique is protein-protein interaction (PPI) analyses, which is considered valuable in interpreting the function of HPs [ 6 ]. (biomedcentral.com)
  • protein interaction and localization studies in vitro and in vivo. (lu.se)
  • Yet, how these genes are recruited and evolve to mediate plant-insect interaction remains unknown. (bvsalud.org)
  • For this reason, using alternative primers for obtaining the emm sequence is discouraged (see emm typing protocol ). (cdc.gov)
  • Following PCR validation, any PCR products that are produced from the novel Lso haplotypes using the targeted primers will then be subjected to sequence analysis. (usda.gov)
  • 7 Primers designed to amplify the partial groEL gene encoding heat-shock protein of Anaplasma phagocytophilum EphplgroELF (5′-ATGGTATGCAGTTTGATCGC-3′) and EphplgroELR (5′-TCTACTCTGTCTTTGCGTTC-3′) were used and expected to yield a 625-bp product for Anaplasma phagocytophilum and for Anaplasma platys , respectively. (who.int)
  • Direct DNA sequencing was performed using the same PCR primers. (who.int)
  • If the sequence result was of low quality, the amplicon was cloned into a plasmid vector using a TOPO TA cloning kit (Invitrogen, Carlsbad, California) and then sequenced using the primers provided with the kit. (who.int)
  • Missing heritability in genome-wide association studies defines a major problem in genetic analyses of complex biological traits 1 , 2 . (nature.com)
  • The Committee concluded that additional work may be needed to assess the viability of the stocks held in VECTOR, and that further molecular characterization of additional strains may be valuable in helping to identify strains from which further DNA sequences could be determined. (who.int)
  • Because we showed that integration neither affects the levels of viral genes, nor those of virally disrupted human genes, a genome-wide screen was performed to identify human genes which expression is influenced by viral integration and have clinical relevance. (cancerindex.org)
  • There has been several studies related to the development and evolution of immunological processes, but a full scale genome and sequence based analysis has been carried out now, including 893 human genes involved in immune related processes. (lu.se)
  • Even if the interest on this technique by the bioinformatics community has been increased during the last few years, there are not many available simple standalone tools to specifically perform these types of data analysis in an integrated environment. (sciweavers.org)
  • The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. (nhbs.com)
  • Recently, there have been major breakthroughs in sequence technologies coupled with bioinformatics, advancing the prospect of cancer genome analysis. (lu.se)
  • The uni- and multivariate logistic regression analyses were applied to identify factors associated with ever, current (in the previous 30 days) and continued e-cigarette use. (who.int)
  • Certain molecules are always present at very high levels, while other, pathogenic proteins may only be present at very low levels and thus be difficult to detect. (lu.se)
  • The main object of the ImmTree database are the human immune system related genes and the corresponding philogenetic trees which describe the evolutionary past of each ortholog groups. (lu.se)
  • Our approach pairs sparse scATAC-seq data with robust detection of hundreds of cell surface and intracellular protein markers and optional capture of mitochondrial DNA for clonal tracking, capturing three distinct modalities in single cells. (nature.com)
  • Fig. 7: ASAP-seq enables detection of intracellular proteins with barcoded antibodies. (nature.com)
  • The viral core protein retains protease activity, needed for cleavage from a protein precursor. (virology.ws)
  • Multiplexed quantification of proteins and transcripts in single cells. (nature.com)
  • Nuclear magnetic resonance imaging, principle components analyses, and hierarchical clustering will be used to prioritize metabolite qualification and quantification via discriminant analyses of selected metabolites versus representative bioassay extracts for mechanism activities. (dal.ca)
  • Lee (2020) and Frésard (2019) demonstrated clinical utility in adding RNA sequencing to genomic testing for cohorts of individuals with undiagnosed Mendelian disease, increasing the diagnostic yield by 18% and 7.5%, respectively. (jax.org)
  • A putative late gene promoter motif sequence, AGTAT was found in the 3' upstream sequences of these genes. (umass.edu)
  • A very common motif among viral capsid proteins is called the single jelly roll , made up of eight beta strands in two four-stranded sheets. (virology.ws)
  • The extra sequences at the N-termini of viral jelly roll capsid proteins, involved in recognizing the viral genome, likely evolved after the capture of these proteins from cells. (virology.ws)
  • Given this information on the origin of viral capsid proteins, we can modify the three hypotheses for the origin of viruses into one. (virology.ws)
  • We know that viral proteins can be returned to cells, where they serve useful functions. (virology.ws)
  • Turns out, that this protein look exactly like a class II viral fusion protein. (virology.ws)
  • Now, the question if of course - is it of viral origin or is it a cellular protein co-opted by viruses? (virology.ws)
  • The demographics and clinical features of the 35 laboratory-confirmed cases were investigated and viral RNA of 12 cases was sequenced and analysed. (ersjournals.com)
  • Strategies for gene therapy and production of medicines via genetically-modified organisms (expression vectors and viral vectors). (lu.se)
  • Caris molecular profiling performs thorough molecular testing on DNA, RNA and proteins to identify the biomarkers driving a patient's tumor, and to compare the biomarkers with data from clinical studies around the world. (carislifesciences.com)
  • Our comprehensive molecular profiling approach precisely analyzes DNA, RNA, and protein biomarkers, revealing the highest quality molecular blueprint for evidence-based selection of the most appropriate cancer therapy. (carislifesciences.com)
  • A few years ago, researchers believed that they would easily be able to identify individual biomarkers for different diseases, i.e. that one protein would be enough to diagnose a certain disease. (lu.se)
  • All of these mechanisms negatively affect expression of the gene. (jax.org)
  • Two transmembrane domains were predicted in p11.7, and a DNA binding motif was found in p31.7 by amino acid sequence analysis, therefore p11.7 and p31.7 were predicted to be envelope and capsid protein, respectively. (umass.edu)
  • However, it is challenging to incorporate coordinates of non-reference sequences into existing analysis pipelines 16 . (nature.com)
  • Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (nih.gov)