• A dynamic programming algorithm to determine the maximum likelihood alignment of error prone electrophoretic mobility data to the expected fragment mobilities given the consensus sequence and restriction enzymes is derived and used to assess the likelihood of detecting rearrangements in genomic sequencing projects. (aaai.org)
  • The local alignment approach also means that an mRNA can be aligned with a piece of genomic DNA , as is frequently required in genome assembly and analysis. (nih.gov)
  • To identify the mutation responsible for renal disease, we sequenced genomic DNA from an affected animal using the Applied Biosystems SOLiD sequencing platform. (nih.gov)
  • SMASH, a fragmentation and sequencing method for genomic copy number analysis. (sfari.org)
  • Three specific biomolecular techniques are addressed: polymerase chain reaction, microarray comparative genomic hybridization, and whole-genome sequencing. (umd.edu)
  • For microarray, novel pan-genomic design and analysis methods are presented for the characterization of unknown microbial isolates. (umd.edu)
  • School of Young Scientists 'Genomic Sequencing and Data Analysis', organised by Institute of Chemical Biology and Fundamental Medicine SB RAS , is devoted to the problems of analysis of data produced by the high-throughput DNA sequencing. (nsc.ru)
  • Dr. Lin's research interests lie in the development and application of scalable statistical and machine learning methods for the analysis of massive data from the genome, exposome and phenome, including big and complex genetic and genomic, epidemiological and health data. (duke.edu)
  • Target sequence capture has emerged as a powerful method to sequence hundreds or thousands of genomic regions in a cost - and time -efficient approach. (bvsalud.org)
  • Since the development of methods of high-throughput production of gene and protein sequences, the rate of addition of new sequences to the databases increased very rapidly. (wikipedia.org)
  • The development of high-throughput DNA sequencing (also commonly referred to as "Next Generation") techniques have allowed for a more complete characterization of marine microbes to be obtained. (nova.edu)
  • This study aims to use high-throughput sequencing (HTS) and ion chromatography to determine: the compositions of the microbial communities ("microbiomes") present in the port and their changes over a year, understand how water chemistry changes may correlate with changes in the microbial composition, and determine which harmful human and marine pathogens are present in the port. (nova.edu)
  • Chipster integrates a comprehensive collection of analysis tools for high-throughput data such as NGS, microarrays and proteomics. (csc.fi)
  • Although high-throughput analysis of liquid biopsies has uncovered many differentially expressed genes in the context of cancer, the functional connection between these genes is not investigated in depth. (frontiersin.org)
  • In 1969 the analysis of sequences of transfer RNAs was used to infer residue interactions from correlated changes in the nucleotide sequences, giving rise to a model of the tRNA secondary structure. (wikipedia.org)
  • There are millions of protein and nucleotide sequences known. (wikipedia.org)
  • When compared by phylogenetic analysis, the nucleotide sequences of FIPV and FECV M genes distributed into paraphyletic patterns rather than in monophyletic clusters (Figure, panel A). Thus, as we observed earlier for the 3c gene ( 10 ), M gene sequences generally clustered according to the cattery from where they originated, irrespective of their pathotype (e.g. (cdc.gov)
  • In some PCR reactions multiple sequences have identical nucleotide sequences, for example if five PCR products have identical sequences a 5 will be found in this column. (cdc.gov)
  • And when they looked at the distribution of the African pan-genome sequences in genomes from a dozen more African individuals and a dozen European individuals enrolled in the Simons Genome Diversity Project, the investigators found that while some of the sequences turned up in individuals of European ancestry, they were far more common in the African individuals profiled. (genomeweb.com)
  • It also identified 20 candidate loci for natural selection where genome sequences were altered as a result of survival and adaptation to local environments during human evolution. (medicalxpress.com)
  • In bioinformatics, sequence analysis is the process of subjecting a DNA, RNA or peptide sequence to any of a wide range of analytical methods to understand its features, function, structure, or evolution. (wikipedia.org)
  • In social sciences and in sociology in particular, sequence methods are increasingly used to study life-course and career trajectories, time use, patterns of organizational and national development, conversation and interaction structure, and the problem of work/family synchrony. (wikipedia.org)
  • Weighing each method's performance and ease, we identified the dUTP second-strand marking and the Illumina RNA ligation methods as the leading protocols, with the former benefitting from the current availability of paired-end sequencing. (nature.com)
  • These methods are potentially the future of diagnostics, but each requires sophisticated computational design or analysis to operate effectively. (umd.edu)
  • This dissertation presents novel computational methods that unlock the potential of these diagnostics by efficiently analyzing whole-genome DNA sequences. (umd.edu)
  • Finally, multiple methods are presented for the validation of whole-genome sequence assemblies, which are capable of identifying assembly errors in even finished genomes. (umd.edu)
  • The drawback is that the amount of data generated in the process is large and extracting useful information from it requires new methods to process and analyze it.The main contribution of this thesis is the development of a novel experimental method coined tagRNA-seq, combining 5'tagRACE, a previously developed technique, with RNA-sequencing technology. (dissertations.se)
  • Examples of her current research include analytic methods and applications for large scale Whole Genome Sequencing studies, biobanks and Electronic Health Records, techniques and tools for whole genome variant functional annotations, analysis of the interplay of genes and environment, multiple phenotype analysis, polygenic risk prediction and heritability estimation. (duke.edu)
  • Our analysis has been the amino acid sequences in proteins differ from what is carried out using two different methods, which differ substantially expected from random sequences in a statistically significant from what is used in ref. 3, although the starting point is similar. (lu.se)
  • An alignment of the relevant part of the polypeptide sequence, comprising the presumed signature residues at positions 108, 120, 138, 163 and 199, is shown in the Figure, panel B, for all FIPV and FECV genomes sequenced in this study. (cdc.gov)
  • The researchers brought together nearly 1.2 trillion paired-end reads for the analysis - the 910 individuals included in the study had their genomes sequenced to an average depth of 30- to 40-fold by the Consortium on Asthma among African-Ancestry Populations in the Americas (CAAPA). (genomeweb.com)
  • Since the very first sequences of the insulin protein were characterized by Fred Sanger in 1951, biologists have been trying to use this knowledge to understand the function of molecules. (wikipedia.org)
  • The method used in this study, which is called the "Sanger method" or Sanger sequencing, was a milestone in sequencing long strand molecules such as DNA. (wikipedia.org)
  • Below are instructions on how to register to iLab and create a service request for Sanger and fragment analysis services. (helsinki.fi)
  • Studies of index cases was done with MISEQ-ILLUMINA, and family screenings and confirmation studies of mutations was done by Sanger sequencing. (degruyter.com)
  • Initial sequencing and comparative analysis of the mouse genome. (bsc.es)
  • The dog genome: survey sequencing and comparative analysis. (jcvi.org)
  • 1.5x coverage) demonstrates the power of sample sequencing for comparative analysis of mammalian genomes and the generation of species-specific resources. (jcvi.org)
  • A common use for pairwise sequence alignment is to take a sequence of interest and compare it to all known sequences in a database to identify homologous sequences. (wikipedia.org)
  • Southern analysis suggests that each gene maps to a different TrIV genome segment, with homologous sequences apparently present on other segments. (gc.ca)
  • CLIMB has proved pivotal to this, providing the computing infrastructure and bioinformatics analysis capability that has so far helped sequence more than 100,000 virus genomes. (cardiff.ac.uk)
  • This self-paced, introductory course, aimed at professionals who want to break into the sequencing-related field of bioinformatics, explores important public access tools used for analyzing biological sequence data. (ucsc-extension.edu)
  • Seven Bridges GRAF™ Suite comprises bioinformatics workflows and tools for secondary analysis of next generation sequencing (NGS) data, based on a pan-genome graph reference. (sevenbridges.com)
  • The latest schedule for the course Bioinformatics and sequence analysis in TimeEdit. (lu.se)
  • Moreover, utilizing advanced bioinformatics tools, the project will employ Gene Set Enrichment Analysis (GSEA) to discern the enrichment patterns of HIF-2α target upregulated genes within the obtained datasets. (lu.se)
  • See how JMP Genomics can help deploy single-cell RNA-sequencing technologies (scRNA-seq) to uncover new and rare cell populations, track trajectories of distinct cell lineages in development, and reveal differentially expressed genes between specific cell types. (jmp.com)
  • This course covers several aspects of NGS data analysis and visualization, ranging from quality control, filtering and alignment to more specific tasks such as finding differentially expressed genes and detecting copy number aberrations, variants, methylation and ChIP-seq peaks. (csc.fi)
  • Methodologies used include sequence alignment, searches against biological databases, and others. (wikipedia.org)
  • Nowadays, there are many tools and techniques that provide the sequence comparisons (sequence alignment) and analyze the alignment product to understand its biology. (wikipedia.org)
  • Relationships between these sequences are usually discovered by aligning them together and assigning this alignment a score. (wikipedia.org)
  • There are two main types of sequence alignment. (wikipedia.org)
  • Pair-wise sequence alignment only compares two sequences at a time and multiple sequence alignment compares many sequences. (wikipedia.org)
  • There are many ways of performing a sequence similarity search, but probably the most popular method is the "Basic Local Alignment Search Tool" ( BLAST ) ( 1 , 2 ). (nih.gov)
  • If instead BLAST started out by attempting to align two sequences over their entire lengths (known as a global alignment), fewer similarities would be detected, especially with respect to domains and motifs. (nih.gov)
  • The megaBLAST nucleotide-nucleotide search, optimized for very similar sequences (in the same or in closely related species), first looks for an exact match of 28 bases, and then attempts to extend that initial match into a full alignment ( 3 , 4 ). (nih.gov)
  • It placed the 296.5 million bases of sequences that resisted alignment with the reference genome into 125,715 contigs, including 33,599 contigs present in the genomes of two or more of the CAAPA participants and tens of thousands of contigs that closely resembled sequences in Korean or Chinese genomes. (genomeweb.com)
  • Conclusion BSBolt offers streamlined processing of bisulfite sequencing data through an integrated toolset that offers support for simulation, alignment, methylation calling, and data aggregation. (biorxiv.org)
  • Sequencing and alignment of this region in 11 cancer cell lines and 4 normal cell lines were also performed, followed by cloning, mutation and luciferase assay analysis. (jcancer.org)
  • In this course you will be provided with both a practical and a theoretical overview of database searching, sequence alignment, phylogenetic and gene prediction, and genome analysis. (lu.se)
  • Strand-specific, massively parallel cDNA sequencing (RNA-seq) is a powerful tool for transcript discovery, genome annotation and expression profiling. (nature.com)
  • Thus, sequence analysis can be used to assign function to genes and proteins by the study of the similarities between the compared sequences. (wikipedia.org)
  • In genetics and genomics research, gene expression refers to the process by which information encoded in a gene's DNA sequence is converted into functional proteins or RNA molecules. (pharmiweb.com)
  • In search for global principles that may explain the organization of the space of all possible proteins, we study all known protein sequences and structures. (aaai.org)
  • This organization extends our ability to predict the structure and function of many proteins beyond what is possible with existing tools for sequence analysis. (aaai.org)
  • Learn how to use a basic Single-Cell RNA-Seq workflow to perform standard exploration on a scRNA-seq data set, investigate customized marker genes, and launch ANOVA process for Differential Gene Expression analysis. (jmp.com)
  • While many flavors of single-cell sequencing have been developed, single-cell RNA sequencing (scRNA-seq) is currently the most prolific form in published literature. (techscience.com)
  • Bioinformatic analysis of differential biology within the population of cells studied relies on inferences and grouping of cells due to the spotty nature of data within individual cell scRNA-seq gene counts. (techscience.com)
  • Authors are encouraged to attempt these aneuploid analyses when reporting scRNA-seq data, much like copy-number variants are commonly reported in bulk genome sequencing data. (techscience.com)
  • In this viewpoint, we direct investigators to select scRNA-seq CNA data analysis tools. (techscience.com)
  • Revealing the evolution and genetic diversity of sequences and organisms Identification of molecular structure from sequence alone In chemistry, sequence analysis comprises techniques used to determine the sequence of a polymer formed of several monomers (see Sequence analysis of synthetic polymers). (wikipedia.org)
  • Genetic mapping by bulk segregation analysis excluded all mutations but this one as the cause of renal disease in Aoba mice. (nih.gov)
  • DGE analysis provides critical insights into the genetic programs governing various stages of development. (pharmiweb.com)
  • Analyses of copy number variants (CNVs) have contributed significantly to our understanding of the genetic etiology of a variety of diseases, including autism spectrum disorder (ASD). (sfari.org)
  • The DNA sequence of a 225.4 kilobase segment of the Escherichia coli K-12 genome is described here, from 76.0 to 81.5 minutes on the genetic map. (nih.gov)
  • Very high frequency of novel mutations (42%) in our study population, supports that in heterogenous disorders like MODY sequence analysis provides rapid, cost effective and accurate genetic diagnosis. (degruyter.com)
  • A new genetic databank has been established containing the completed whole-genome sequencing (WGS) data of close to 5,000 Singaporeans. (medicalxpress.com)
  • The representation of the human reference genome as a linear haploid DNA sequence poses limitations when trying to incorporate the known genetic diversity of human populations. (sevenbridges.com)
  • We used whole- exome sequencing (WES) to evaluate the presence of genetic variants in developmental disorder genes (diagnostic genetic variants) in a cohort of fetuses with structural anomalies and samples from their parents , after exclusion of aneuploidy and large CNVs. (bvsalud.org)
  • Background Bisulfite sequencing is commonly employed to measure DNA methylation. (biorxiv.org)
  • Loci lacking molecular data for a large number of samples are commonly excluded from downstream analyses, even though they may still contain valuable information. (bvsalud.org)
  • Through a whole-exome sequencing-based analysis of rare CNVs in 98 high-risk Northern Finnish breast cancer cases, the researchers aimed to tease out the role in breast cancer risk. (genomeweb.com)
  • Detection of CNVs has been limited, in part due to the significant costs associated with both chromosomal microarray analyses (CMA) and whole-genome sequencing (WGS). (sfari.org)
  • Testing for aneuploidy and CNVs is routine during the investigation of fetal structural anomalies, but there is little information on the clinical usefulness of genome -wide next-generation sequencing in the prenatal setting. (bvsalud.org)
  • NEW YORK (GenomeWeb) - Using genome sequence data from more than 900 individuals of African ancestry, a team led by researchers at Johns Hopkins University has identified hundreds of millions of bases that are not represented in the human reference genome. (genomeweb.com)
  • The researchers analyzed deep whole-genome sequence data from 910 previously sequenced African individuals, uncovering more than 296 million bases of sequence that did not align to version GRCh38 of the human reference genome. (genomeweb.com)
  • The team's analysis suggested that an African pan-genome bolstered by these new sequences contained roughly 10 percent more DNA than found in the GRCh38 human reference genome. (genomeweb.com)
  • The first example describes a research project involving sequencing based gene expression analysis and the second example describes a microarray service facility use of BASE. (lu.se)
  • In general, the matches in the database are ordered to show the most closely related sequences first, followed by sequences with diminishing similarity. (wikipedia.org)
  • A sequence similarity search often provides the first information about a new DNA or protein sequence. (nih.gov)
  • The BLAST algorithm is tuned to find these domains or shorter stretches of sequence similarity. (nih.gov)
  • Find clusters of DNA sequences based on their global similarity to two reference sequences. (wolfram.com)
  • How to Do Differential Gene Expression Analysis? (pharmiweb.com)
  • Differential Gene Expression analysis is a fundamental tool for identifying genes whose expression levels significantly differ between experimental conditions or biological samples. (pharmiweb.com)
  • In comparison to the frequently used differential gene expression analysis the approach offers additional insights by incorporating information about the relationship between genes detected in a given sample. (frontiersin.org)
  • Additionally, they discuss recent developments in library preparation and data analysis tools further streamlining the sequencing process that integrate workflows in laboratories or in remote field scenarios. (ojp.gov)
  • Users can save their analysis pipelines as reusable automatic workflows, and both workflows and analysis sessions can be shared with colleagues. (csc.fi)
  • As they reported today in Nature Genetics , the researchers then assembled those non-reference sequences into more than 125,700 "African pan-genome contigs," including hundreds of contigs that overlapped with and altered protein-coding genes. (genomeweb.com)
  • Maturity onset diabetes of the young in India - a distinctive mutation pattern identified through targeted next-generation sequencing. (degruyter.com)
  • 9 ). Their conclusion was based on sequence analyses of parts of the viral genome including the matrix (M) gene, phylogenetic analysis of which revealed reciprocal monophyly of the sequences obtained from FIP cases versus those of asymptomatic FECV-infected animals. (cdc.gov)
  • Phylogenetic analysis of the three aspergilli using the whole-genome data showed that A. nidulans branched off earlier than A. oryzae and A. fumigatus 5 . (nature.com)
  • Phylogenetic analysis of Bma-RXR and related nuclear receptor sequences. (figshare.com)
  • Our study demonstrates the speed and utility of whole genome sequencing coupled with low resolution meiotic mapping as a means of identifying causative mutations induced by ENU. (nih.gov)
  • Our study thus demonstrates the feasibility of performing gene co-expression network analysis from cell-free RNA data and its utility in studying hepatocellular carcinoma. (frontiersin.org)
  • Target sequence capture of Barnadesioideae (Compositae) demonstrates the utility of low coverage loci in phylogenomic analyses. (bvsalud.org)
  • The CORALL™ Total RNA-Seq V2 Library Prep kits from Lexogen enables easy and cost-efficient generation of UMI labelled, stranded libraries for whole transcriptome analysis using Illumina® next generation sequencing (NGS) platforms. (bioscience.co.uk)
  • Through hands-on examples and exercises, you will learn how to access public databases for raw sequence data and perform the basic steps in processing next-generation sequence data for RNA, DNA, and ChIP sequencing data to obtain interpretable results. (ucsc-extension.edu)
  • The aim of this study is to investigate both novel and proven mutations of 11 MODY genes in Turkish children by using targeted next generation sequencing. (degruyter.com)
  • Phenotypic heterogeneity in monogenic diabetes: The clinical and diagnostic utility of a gene panel-based next-generation sequencing approach. (degruyter.com)
  • Aneuploidy, the loss or gain of chromosome arms, appears less prevalent in normal tissue in these clonal mutant next-generation sequencing analyses. (techscience.com)
  • Next Generation Sequencing (NGS) is a new technology that has revolutionized the way we study living organisms. (dissertations.se)
  • Deep sequencing and SNP array analyses of pediatric T-cell acute lymphoblastic leukemia reveal NOTCH1 mutations in minor subclones and a high incidence of uniparental isodisomies affecting CDKN2A. (lu.se)
  • Sequence analysis detects mutations in 95% of males and 95% of female carriers. (medscape.com)
  • which are detectable by chromosomal microarrays), and pathogenic sequence variants in developmental genes . (bvsalud.org)
  • Processing bisulfite sequencing data is often challenging due to the computational demands of mapping a low complexity, asymmetrical library and the lack of a unified processing toolset to produce an analysis ready methylation matrix from read alignments. (biorxiv.org)
  • We found that BSBolt provides accurate and fast bisulfite sequencing alignments and methylation calls. (biorxiv.org)
  • Our analysis provides a comprehensive benchmark, and our computational pipeline is applicable for assessment of future protocols in other organisms. (nature.com)
  • A computational system set up for COVID-19 sequencing analysis has won a major award. (cardiff.ac.uk)
  • Computational nodes are connected to the main computer and used to run configured analysis plugins in a seamless integrated fashion. (lu.se)
  • Approximately 74% of the nucleotides comprising coding sequences and splice junctions in the mouse genome were covered at least three times. (nih.gov)
  • Ab initio construction of a eukaryotic transcriptome by massively parallel mRNA sequencing. (nature.com)
  • A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome. (nature.com)
  • This includes trimming adapter sequences, filtering low-quality reads, and aligning the reads to a reference genome or transcriptome. (pharmiweb.com)
  • Analysis and execution of standard analysis pipelines for sequencing data will be performed through BASE. (lu.se)
  • Reads most sequence formats. (cnet.com)
  • DNA sequence analysis depends on the accurate assembly of fragment reads for the determination of a consensus sequence. (aaai.org)
  • However, this analysis requires accurate reference genomes to identify the specific genes from which RNA reads originate. (osti.gov)
  • Briefly, tagRNA-seq makes it possible to identify the 5' ends of RNAs in bacteria and directly probe for their type, primary or processed, by ligating short RNA sequences, the tags, to the beginnings of RNA molecules. (dissertations.se)
  • Two important algorithms for aligning pairs of sequences are the Needleman-Wunsch algorithm and the Smith-Waterman algorithm. (wikipedia.org)
  • There are numerous algorithms available as freeware or by public access in the cloud that make complex biological sequence analyses accessible to everyone. (ucsc-extension.edu)
  • For designing real-time detection assays, an efficient data structure and search algorithm are presented to identify the most distinguishing sequences of a pathogen that are absent from all other sequenced genomes. (umd.edu)
  • This recognition comes as ultra-deep sequencing technologies coupled with error-reducing sequencing techniques have enabled the detection of mutant cells occupying less than one percent of an examined tissue sample ( Martincorena, 2019 ). (techscience.com)
  • Sequence-specific, single primer amplification and detection of PCR products for identification of hepatitis viruses. (cdc.gov)
  • We report on the cloning and sequencing of two Tranosema rostrale ichnovirus (TrIV) genes, and assess their relatedness to TrV1, the gene encoding the most abundant TrIV transcript in last-instar Choristoneura fumiferana larvae parasitized by T. rostrale . (gc.ca)
  • This suggests that the genome size differences are largely due to sequence acquisition in A. oryzae . (nature.com)
  • In addition, such an analysis should avoid biases in transcript counts related to differences in organism abundance. (osti.gov)
  • In addition to the literature review of behavior analytic studies, we examine some conceptual differences between Behavior Analysis and cognitive approaches and provide explanations about these differences. (bvsalud.org)
  • An underexplored way of analyzing the rich data obtained from sequencing liquid biopsy-derived cfRNA liquid biopsy is weighted gene co-expression network analysis (WGCNA). (frontiersin.org)
  • Analyses of RNA-seq data obtained in the context of these experiments revealed subtle artifacts in the coverage signal towards gene ends, that we were able to explain and quantify based Kolmogorov's broken stick model. (dissertations.se)
  • Results are presented that show these "signature" sequences can be used to detect pathogens in complex samples and differentiate them from their non-pathogenic, phylogenetic near neighbors. (umd.edu)
  • The results showed that there were four possible HREs (HRE1-4) sequences in 1275bp range before ATG start codon. (jcancer.org)
  • Sequencing results were interpreted with a targeted virtual gene panel for developmental disorders that comprised 1628 genes . (bvsalud.org)
  • The possible hypoxia response element (HRE) sequences in 5' non-coding Region before ATG start codon of Sema4D were screened, followed by point mutagenesis and luciferase assay analysis. (jcancer.org)
  • Using available RNA sequencing data from previously published studies on intestinal organoids, the project will compare the gene expression profiles with newly generated RNA sequencing data from our lab's organoid cultures at hypoxia. (lu.se)
  • Hence, our data do not confirm the diagnostic potential of the M protein sequence nor do they support the suggested role of the membrane protein in FIP pathogenesis ( 9 ). (cdc.gov)
  • Doesn't appear to be from Blast but try blasting the DNA sequence rather than protein sequence. (bio.net)
  • BLASTP performs protein-protein sequence comparison, and its algorithm is the basis of many other types of BLAST searches such as BLASTX and TBLASTN . (nih.gov)
  • Each row corresponds to a specific residue and describes the probability of related sequences having that residue at that position. (nih.gov)
  • Our analysis revealed a number of genes previously associated with hepatocellular carcinoma and uncovered their association network in the blood. (frontiersin.org)
  • On the other hand , including data-poor loci may bias phylogenetic analyses . (bvsalud.org)
  • Here we use a target sequence capture dataset of an ecologically and taxonomically diverse group of spiny sunflowers ( Asteraceae , or Compositae Barnadesioideae) to test how the inclusion or exclusion of such data-poor loci affects phylogenetic inference . (bvsalud.org)
  • We investigate the sensitivity of concatenation and coalescent approaches to missing data with matrices of varying taxonomic completeness by filtering loci with different proportions of missing samples prior to data analysis . (bvsalud.org)
  • With built-in UMIs and 384 UDIs for maximised sequencing output, and an all-in-one protocol, this technology allows for the complete and uniform transcript coverage from start to end, and flexible input requirements (1ng to 1 μg). (bioscience.co.uk)
  • As a starting material we use purified PCR products mixed with sequencing primer or ready made sequencing/fragment analysis reactions. (helsinki.fi)
  • By subjecting DEGs to gene ontology enrichment analysis, pathway analysis, or functional annotation, we gain insights into the biological processes, molecular functions, and pathways associated with the observed gene expression changes. (pharmiweb.com)
  • In this talk, I will discuss the analytic tools and resources for scalable analysis of large scale biobank- and population-based Whole Genome Sequencing (WGS) association studies of common and rare variants by integrating WGS data with multi-faceted functional annotation data. (duke.edu)
  • Discussions include fitting mixed models for continuous and discrete and survival phenotypes using sparse GRM in population and biobank based studies, and rare variant association tests and meta-analysis by incorporating multi-faceted variant functional annotations including single-cell-based cell-specific annotations using individual level data and WGS summary statistics. (duke.edu)
  • Oxford Nanopore Technologies (ONT) has developed a portable, nanopore-based sequencing instrument that has already been utilized for on-site identification of Zika and Ebola viruses, full genome sequencing, evaluation of DNA and RNA base modifications, and enrichment-free mitochondrial DNA analysis. (ojp.gov)
  • a fast and scalable bisulfite sequencing analysis platform. (biorxiv.org)
  • To date, many studies have focused on WGCNA analysis of bulk or single-cell RNA (scRNA) sequencing data from tissue samples. (frontiersin.org)
  • Most job postings for technicians and scientists in the molecular biology field ask for experience with next-gen sequencing. (ucsc-extension.edu)
  • 9 ). GenBank accession numbers for the FCoV M gene sequences determined in this study are HQ738691-HQ738733. (cdc.gov)
  • The objective of this study was to identify and map seismic horizons that are continuous throughout the entire area in order to generate a chronostratigraphic framework for the syn-kinematic succession, and then to define seismic facies in the interpreted sequences that would help to understand the depositional environments, and therefore, to predict the distribution of reservoir, source and seal rocks. (aapg.org)
  • Our study describes the sequence characteristics of 5' non-coding region of Sema4D, enhances our understanding of the regulatory mechanism of Sema4D and benefits the development of a possible anti-angiogenesis therapeutic strategy for malignancies. (jcancer.org)
  • To describe studies referring to stimulus equivalence, we present two approaches for the study of verbal behavior sequencing. (bvsalud.org)
  • Prenatal exome sequencing analysis in fetal structural anomalies detected by ultrasonography (PAGE): a cohort study. (bvsalud.org)
  • A search allows scientists to infer the function of a sequence from similar sequences. (nih.gov)
  • The physiological conditions experienced by a microbial community can thus be inferred using meta-transcriptomic sequencing by comparing transcription levels of specifically chosen genes. (osti.gov)
  • 5) Emigration of second-generation moths was confirmed by both radar observation and mtDNA analysis. (bioone.org)
  • Throughout these studies, one feature of verbal behavior presented itself as a common and essential issue: verbal behavior sequencing/ordering. (bvsalud.org)
  • while in Behavior Analysis, this issue is presented in a fragmented way and under different perspectives. (bvsalud.org)
  • In light of this, the purpose of this paper was to present a review of behavior analytic explanations of the acquisition, maintenance, and generalization of sequencing verbal behavior repertoires while identifying how this analysis can provide alternative explanations to the findings from cognitive studies, especially the ones dedicated to storytelling behaviors. (bvsalud.org)
  • The analysis showed that the body of literature produced by cognitive approaches is, in many aspects, in opposition to the one produced by Behavior Analysis. (bvsalud.org)
  • When presenting Skinner's explanation of sequencing verbal behavior, we indicate that this explanation is based on speakers' repertoires, on the search for variables that control the verbal operants, and on the reinforcing practices of the verbal community. (bvsalud.org)
  • formed a Fourier analysis based on the random walk represen- erties in terms of thermodynamical and kinetic behavior, tation. (lu.se)