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  • Assay
  • Use at an assay dependent concentration. (abcam.com)
  • It would be advantageous if one could combine screening of protein-protein interactions with tests for biological relevance by using a single assay system, thus validating the screening results and eliminating spurious interactions immediately. (pnas.org)
  • We have demonstrated that the DHFR PCA can be used as a sensitive survival-selection assay and also as a fluorescence assay that allows for quantitative detection of induced protein-protein interactions ( 4 , 5 ). (pnas.org)
  • If we observe an interaction between two proteins in a simple screen (survival-selection assay), what additional information must we generate, for example, using the fluorescence assay, to show that the interaction is biologically relevant? (pnas.org)
  • molecular
  • Click molecule labels to explore molecular sequence information. (nih.gov)
  • When it was found that the antibiotic rapamycin was a potent inhibitor of the proliferation of virtually every eukaryotic cell type examined (e.g., fungi, T cells, and tumor cells) [ 1 , 2 , 3 ], it became clear that the molecular target of rapamycin must be highly conserved and its function critical for cell survival. (mdpi.com)
  • ENTH
  • They contain the ENTH and ANTH (E/ANTH) domain that binds both inositol phospholipids and proteins that contribute to the nucleation and formation of clathrin coats on membranes [ PMID: 14657269 ]. (ebi.ac.uk)
  • ENTH/ANTH proteins and clathrin-mediated membrane budding. (ebi.ac.uk)
  • membrane
  • The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G proteins than PIK3CG:PIK3R5 and its membrane recruitment and beta-gamma G protein dimer-dependent activation requires HRAS bound to PIK3CG. (uniprot.org)
  • The association of PCTAIRE-like proteins with cyclins has not been widely studied, although PFTAIRE-1 has been shown to function as a CDK which is regulated by cyclin D3 as well as the membrane-associated cyclin Y. PCTAIRE-like proteins show unusual expression patterns with high levels in post-mitotic tissues, suggesting that they may be involved in regulating post-mitotic cellular events. (umbc.edu)
  • multiple proteins
  • Thus, ELMs and SLiMs are both identified as sequence patterns in multiple proteins that bind to a common target, with the SLiM-containing set likely to be entirely non-homologous but with no such restriction on the ELM-containing set. (biomedcentral.com)
  • bind
  • Many well represented domains recognize and bind to primary sequences less than 10 amino acids in length called Short Linear Motifs (SLiMs). (biomedcentral.com)
  • Short linear motifs (SLiMs) are also identified as specific sequence patterns that are over-represented in proteins that bind to a common partner, but the algorithms used to discover SLiMs employ filters to remove homologous proteins whereas the ELM-discovery algorithms do not. (biomedcentral.com)
  • This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. (nih.gov)
  • regulate
  • 14-3-3 binding to phosphorylated Ser 776 is proposed to regulate the interactions of ataxin-1 with two protein complexes, one including splicing factors and the other containing capicúa [ 10 , 15 , 16 ]. (biochemj.org)
  • Mutations
  • Lack of capicúa enables Drosophila cells to grow without Ras function, but does not compensate for growth defects due to mutations in insulin/PKB (protein kinase B, Akt) signalling [ 6 ]. (biochemj.org)
  • functional
  • This trend is more evident in those protein functional groups that are frequently reported to interact with specific domains. (biomedcentral.com)
  • The correlation between SLiM conservation with disorder prediction demonstrates that functional SLiMs recognized by each domain occur more often in disordered as compared to structured regions of proteins. (biomedcentral.com)
  • We report an experimental strategy that allows for detection of protein interactions and functional assays with a single reporter system. (pnas.org)
  • occurs
  • It often occurs in young females, and is the most common thyroid cancer in children and patients who have undergone radiation therapy to the head and neck ( 3 ). (spandidos-publications.com)
  • PMID
  • Yeast Sla2p has been extensively studied: Sla2p arrives at existing endocytic patches with a ~25 seconds delay relative to clathrin and dissociates simultaneously with clathrin upon recruitment of actin-related proteins [ PMID: 15059611 , PMID: 9362070 ]. (ebi.ac.uk)
  • amino
  • Early eluting fractions were subjected to amino acid sequence analysis by reverse-phase LC-MS/MS with data-dependent MS/MS/MS acquisition. (pnas.org)