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GelsChromatography, High Pressure LiquidChromatography, AffinityChromatography, GelChromatographyChromatography, Ion ExchangeChromatography, GasChromatography, LiquidChromatography, Thin LayerChromatography, DEAE-CelluloseMolecular WeightMolecular Sequence DataElectrophoresis, Polyacrylamide GelMass SpectrometryElectrophoresis, Gel, Two-DimensionalAmino Acid SequenceKineticsChromatography, AgaroseChromatography, Reverse-PhaseAnxiety, SeparationHydrogen-Ion ConcentrationGas Chromatography-Mass SpectrometryIsoelectric FocusingCountercurrent DistributionImmunomagnetic SeparationCell SeparationElectrophoresis, Agar GelAmino AcidsElectrophoresis, CapillaryChromatography, PaperSepharoseChemical FractionationSubstrate SpecificityElectrophoresis, Gel, Pulsed-FieldElectrophoresisSpectrophotometry, UltravioletTandem Mass SpectrometrySpectrometry, Mass, Electrospray IonizationChromatography, Micellar Electrokinetic CapillaryMethodsTemperatureCattleEscherichia coliBase SequenceRabbitsChemistryCentrifugation, Density GradientChemical PhenomenaReproducibility of ResultsProtein BindingElectrophoresis, MicrochipIndicators and ReagentsMaternal DeprivationTrypsinPeptide FragmentsDrug StabilityLiverPeptidesHydrolysisCarbohydratesMagnetic Resonance SpectroscopyAcetonitrilesStereoisomerismLimit of DetectionBinding SitesBacterial ProteinsMicrochemistryRecombinant ProteinsTime FactorsDNAProteinsCloning, MolecularReference StandardsCalibrationSpecies SpecificityChemical PrecipitationCell LineIsoelectric PointHydroxyapatitesSensitivity and SpecificityCarbohydrate SequenceSolubilityProteomicsSilicon DioxideMacromolecular SubstancesRadioimmunoassayIsomerismOligosaccharidesSolventsUltracentrifugationHot TemperatureChemistry Techniques, AnalyticalSwinePolysaccharidesPhysicochemical PhenomenaCell MembraneChemistry, PhysicalMolecular StructureIsoenzymesSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Liquid chromatographyThin-layer chromatoHPLCPermeation chromatographySilicaTypes of chromatographySupercritical fluid chromatoPurificationProteinsMass spectrometryHydrophilicAdsorptionChromatographic separationAnalyticalInteractionPhasesPreparativePore structureAnalyteDetectionWidelySeparateSuitableMixturesPolymersMoleculesPrinciple of separationEfficiencyIonicColumnAnalysisAffinityTechniqueRetention mechanismsPolymerSizeTechniquesParticleAdsorbentQuantifyMethodColumnsChemicalAcidic
Liquid chromatography33
Thin-layer chromato7
HPLC14
  • During development of a silver ion HPLC method to determine the structural integrity of synthesized monoacyl (MAG) and diacylglycerols (DAG), the retention characteristics of fatty acid methyl esters (FAME) and triacylglycerols (TAG) were found to be influenced not only by the number and/or configuration of the double bonds in the molecules, but also by the number of carbon atoms in the fatty acid (FA) chains. (usda.gov)
  • Because of the wide assortment of specialized stationary phases and the vast number of reagents that can be used, there is almost an infinite number of ways a scientist can customize the setup of a HPLC system to achieve the desired analyte separation. (coleparmer.com)
  • While HPLC separations are often not as efficient as GC separations, one big advantage of using HPLC is that the sample components can be reused and analyzed further if needed. (coleparmer.com)
  • Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology. (sigmaaldrich.com)
  • See how factors relate to particle and column characteristics via the HPLC resolution equation. (sigmaaldrich.com)
  • Supel™ Carbon LC U/HPLC columns packed with porous graphitic carbon (PGC) particles, used for the HPLC, UHPLC, UPLC, and LC-MS separation of polar or charged compounds at high temperatures (up to 250 ˚), pressures up to 700 bar, and the mobile phase pH range of 1-14. (sigmaaldrich.com)
  • HPLC separation of 17 important cannabinoids including CBD, delta 9 THC and THCA. (sigmaaldrich.com)
  • The HPLC analysis of small molecules most often is performed in reversed-phase separation mode. (sigmaaldrich.com)
  • A comparative study of AF4 with size exclusion chromatography (SE-HPLC) unveiled SE-HPLC to inhere higher recovery rates and AF4 to exhibit greater resolution. (uni-muenchen.de)
  • From gas chromatography and HPLC to ion-exchange, gel permeation and affinity, there are various different types of chromatography to choose from. (chromatographytoday.com)
  • High efficiency separations at any pH The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH. (thomassci.com)
  • SUMICHIRAL OA columns are high-performance chiral columns for enantiomer separation by HPLC. (thomassci.com)
  • It is well suited for the separation of neutral, acidic and weak basic compounds.This sorbent is packed into a Hibar® 125-4 HPLC column. (thomassci.com)
  • Due to low bleeding characteristics this HPLC phase is suitable for LC/MS. (mn-net.com)
Permeation chromatography5
  • The full name of the GPC system is gel permeation chromatography system . (antiteck.com)
  • It is a relatively traditional separation technique, based on the principle of gel permeation chromatography to separate and collect complex samples according to the size of the molecular volume, which can effectively remove the large molecular matrix and small molecules in the sample, improve the sensitivity and accuracy of subsequent analysis, and extend the service life of analytical instruments. (antiteck.com)
  • Gel permeation chromatography system working principle , on which the gel purification system works, separates and collects complex samples according to the size of the solute molecules. (antiteck.com)
  • Infrared Spectroscopy coupled with a separation technique like Gel Permeation Chromatography (GPC) or Size Exclusion Chromatography (SEC) is well suited to determine the compositional and structural arrangement of polymers or copolymers. (spectra-analysis.com)
  • For example, in gel permeation chromatography, special inert beads are used as the stationary phase, and the mobile phase carries the analyte past those beads. (coleparmer.com)
Silica17
Types of chromatography2
Supercritical fluid chromato2
Purification10
  • And it is a fast and inexpensive technology compared with traditional chromatography, which is widely used in drug research, sample purification, and purification of natural products currently. (flashcolumns.com)
  • The characteristics of low-cost and simplicity result in its irreplaceable in the separation and purification. (flashcolumns.com)
  • 2. Use column chromatography above 100 grams to prepare purification technology. (flashcolumns.com)
  • The principle is to use the characteristics of the protein surface to make it adsorbed on the gel column to achieve separation and purification The purpose of protein. (sepuretech.com)
  • A gel purification system with multiple specifications of stainless steel purification column and glass purification column is available, and the glass purification column has a spiral mouth design, which is convenient to adjust the column length. (antiteck.com)
  • GPC system is composed of purification host and liquid workstation, with integrated design, compact and coordinated layout, which can automate sample feeding, separation and purification, target component collection series operations, and control software to perform data acquisition, storage, and management functions. (antiteck.com)
  • Chromatography can be used in a myriad of applications from analyzing miniscule samples to production-scale use as a purification step. (coleparmer.com)
  • This article explores chromatographic separation techniques for the efficient purification of cannabinoids along with the benefits and drawbacks of these separation methods. (cannabissciencetech.com)
  • Preparative column chromatography is used for large-scale purification of compounds. (cannabissciencetech.com)
  • Column chromatography can be used as a large-scale method for purification and separation of the plant extract chemical profile. (cannabissciencetech.com)
Proteins7
  • These three steps are essential in understanding the composition and characteristics of proteins within a sample. (proprofs.com)
  • In this chapter, we will describe how zein proteins influence characteristics of maize grain and nutritional value of maize. (intechopen.com)
  • Students will investigate a range of subjects, including enzyme activity, characteristics, cell location, and the evolution of mammalian proteins, by following the step-by-step methods in their laboratory guides. (modernbio.com)
  • Protein fingerprinting is a popular method for this aim, in which the electrophoretic characteristics of specific proteins are examined in multiple species. (modernbio.com)
  • Proteins that share the common characteristic of binding to carbohydrates . (lookformedical.com)
  • Summary, conclusions and prospective 177 Given the background that effective AF4 fractionations are due to differences in analyte size - i.e., in diffusion coefficients -, the separation of equal-sized proteins is prima facie considered to be impractical. (uni-muenchen.de)
  • Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 10,000 to 1,000,000. (thomassci.com)
Mass spectrometry2
Hydrophilic2
  • For separation of polar compounds hydrophilic interaction chromatography (HILIC) and normal-phase chromatography are suitable as well, with HILIC being the preferred method. (sigmaaldrich.com)
  • The characteristics of the hydrophilic stationary phase may affect and in some cases limit the choices of mobile phase composition, ion strength or buffer pH value available, since mechanisms other than hydrophilic partitioning could potentially occur. (springer.com)
Adsorption3
  • There are several ways to model the separation mechanism: partition, adsorption, ion exchange, and size exclusion. (springer.com)
  • The specific surface area, coupled with controlled pore characteristics, enables targeted and efficient adsorption. (column-chromatography.com)
  • Adsorption and enthalpic partition often function in concert, either synergistically or antagonistically, and this can have a significant impact on polymer separation selectivity. (tsijournals.com)
Chromatographic separation2
  • Flash chromatography is a kind of fast chromatographic separation mode, which separates substances by using optimized pre-packaged medium and low-pressure columns. (flashcolumns.com)
  • Only when a suitable renaturation buffer is selected to make the protein fold correctly, can further chromatographic separation be successfully completed. (medicilon.com)
Analytical2
  • Separation of Salicylic acid, meets analytical specification of Ph. (sigmaaldrich.com)
  • It was the aim of this thesis to evaluate the applicability of asymmetrical flow field flow fractionation (AF4) in pharmaceutical protein analytics, to compare AF4 performance with established state-of-the-art methods and to reveal the effectivity of inherent AF4 characteristics in demanding analytical tasks. (uni-muenchen.de)
Interaction5
  • Silver ion chromatographic separations are generally attributed to the interaction of silver ions with double bond pi-electrons, a condition absent in the 16.0 series. (usda.gov)
  • At its core, chromatography is the interaction between a mobile phase (which carries the mixture being separated) and a stationary phase (which performs the separation). (coleparmer.com)
  • By exploiting these differences, chromatography allows for the separation of compounds based on their various properties, such as size, charge, polarity, and interaction with specific chemical groups. (cannabissciencetech.com)
  • The evaluation of their interaction characteristics with target molecules and use as drugs, optimization of their number of the bases and sequence are critical. (jascoinc.com)
  • When double-stranded DNA (dsDNA) is heated, interaction forces that contribute to the formation of double strand such as hydrogen bonding between the bases pairs is weakened, resulting in the separation of dsDNA into two single-stranded DNA (ssDNA). (jascoinc.com)
Phases5
  • Regardless of how it is used, the overall effectiveness of chromatography largely depends on choosing the right technique and phases to use within that technique. (coleparmer.com)
  • As the mobile phase moves through the stationary phase, the components of the mixture interact differently with the two phases, leading to separation. (cannabissciencetech.com)
  • The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. (springer.com)
  • The modern definition of chromatography is, it is a physicochemical technique of separation in which the compounds that required to be separated are distributed between two phases, one is called stationary phase (which remains stationary), and the other is a mobile phase (which moves through the stationary phase). (tech-publish.com)
  • With Reverse Phase, Normal Phase and Specialty Phases, ProteCol ensures you have the right phase for your separation needs. (thomassci.com)
Preparative1
  • In the first dimension, a XION preparative column was used to separate the flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of flavonoids and nine flavonoid fractions were obtained. (virginia.edu)
Pore structure1
  • The tailored pore structure and specific surface area enhance selective separations, crucial in complex mixtures. (column-chromatography.com)
Analyte2
  • Most chromatography methods have an inert mobile phase that carries the analyte through a long stationary phase housed inside a column. (coleparmer.com)
  • The stationary phase is designed to separate the components of the analyte based on some defined characteristic, which causes some molecules to migrate through the stationary phase more slowly and others to pass through more quickly. (coleparmer.com)
Detection1
  • Quantification of the metabolites DMP, DMTP, and DMDTP in urine samples is typically accomplished using gas chromatography (GC) with nitrogen phosphate detection (NPD) or with flame photometric detection (FPD). (cdc.gov)
Widely3
  • Chromatography, a widely utilized separation technique, offers promising solutions to overcome these challenges. (cannabissciencetech.com)
  • Chromatography is a versatile and widely used separation technique employed to separate and analyze mixtures of chemicals or substances into their individual components. (cannabissciencetech.com)
  • NP-LC has been widely used to separate various compounds, from nonpolar to highly polar compounds (note that chromatography was first introduced as a method used in separation science). (springer.com)
Separate4
Suitable4
  • Since its biological plasma in the electrodes by T. RCF in collaboration of 500,000 g. relating characteristics of this assay, many sizes and cookies of second laboratories of methods in Original or suitable fractions are failed phosphotungstic. (arm-sind-die-anderen.de)
  • Device کرماتوگرافی thin layer, usually in all the laboratories of chemistry as a tool suitable, simple, cheap, and convenient, the direction of separation and isolation, fast and simple, chemicals there. (maharfanabzar.com)
  • It can be said that the choice of the correct and suitable phase motion is the most important part chromatography, TLC will be. (maharfanabzar.com)
  • For example, it is suitable for analyzing compounds in complex systems that always elute near the void in reserved-phase chromatography. (springer.com)
Mixtures1
  • Chromatography is a technique for separating mixtures into their constituent parts. (coleparmer.com)
Polymers1
  • Polymers are defined by the characteristics of their constituent monomer(s) and their microstructural arrangement. (spectra-analysis.com)
Molecules4
  • The atoms or molecules in the sample can be identified by correlating known masses (e.g. an entire molecule) to the identified masses or through a characteristic fragmentation pattern. (wikipedia.org)
  • As a scientific equipment, GPC system in the analysis of pesticide residues and semi-volatile organic compounds in various types of food, agricultural products and aquatic products, in organic sample extracts generally contain large molecules, if these substances are not removed, it will lead to a reduction in the separation efficiency of the column, shorten the life of the injection port and column, and then affect the data analysis results. (antiteck.com)
  • The column packing gel has pores of different sizes, and when component molecules of different sizes enter the gel with the mobile phase, the smaller the molecules are the deeper they enter the micropores and the longer they are retained in the gel, so they can be eluted in order of molecular size. (antiteck.com)
  • In materials science, the sol-gel process is a method for producing solid materials from small molecules. (haevents.us)
Principle of separation1
  • Today, most applications of chromatography are generally less colorful but the underlying principle of separation remains unchanged. (coleparmer.com)
Efficiency1
Ionic2
  • For the separation of ionic compounds, ion-exchange separation modes and for inorganic anions or cations, ion chromatography can also be used. (sigmaaldrich.com)
  • Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
Column12
Analysis4
Affinity4
  • The editor today will talk to you about His-Tag affinity chromatography packing material. (sepuretech.com)
  • According to the different binding groups, Ni2 + affinity chromatography columns can be divided into two categories-Ni-IDA and Ni-NTA. (sepuretech.com)
  • For more inquiries about His-Tag affinity chromatography packing, please continue to pay attention to the official website of Suzhou Sepure Instrument Co., Ltd. www.sepuretech.com! (sepuretech.com)
  • Affinity Chromatography: A Ansroid Approach. (forexsignal300.com)
Technique1
Retention mechanisms1
  • Separation principle based on two retention mechanisms - π-π interactions and hydrophobic interactions. (mn-net.com)
Polymer2
  • Polymer gel separation tube (SST or PST). (nmslabs.com)
  • However, in the presence of a fluctuating second or even third molecular characteristic, GPC/SEC cannot provide information regarding the polymer molar mass. (tsijournals.com)
Size2
  • The typical pore size of the stationary phase material for small molecule separation is in the range of 60 Å - 160 Å. (sigmaaldrich.com)
  • GPC/SEC, or gel permeation (size exclusion) chromatography, is often used to determine the molar mass of macromolecules. (tsijournals.com)
Techniques1
Particle1
  • After that, the characteristic particle and the nature of them are checked. (maharfanabzar.com)
Adsorbent1
  • These UNIPLATES are impregnated with between 5% and 20% silver nitrate which modifies the separation characteristics of the adsorbent layer to permit increased discrimination of certain compounds, particularly those containing carbon-carbon double bonds. (milesscientific.com)
Quantify1
  • For example, chromatography can be used to quantify how much pesticide residue was present in a batch of apple juice or determine how much of an active drug is present in a pill. (coleparmer.com)
Method1
  • The importance of the thermodynamic properties has been highlighted, and therefore an efficient method to analyze these characteristics is required. (jascoinc.com)
Columns3
  • Boasting a bulk density of around 920 g/l, Aluminium Oxide Acidic stands as a stable component within chromatography columns. (column-chromatography.com)
  • On SUMICHIRAL OA columns direct separation of various enantiomers can be realized effectively. (thomassci.com)
  • Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations - without the elevated backpressures required by sub-2µm particles. (thomassci.com)
Chemical2
  • The calculated CI value ensures minimal interference from chemical impurities, maintaining separation integrity. (column-chromatography.com)
  • Thus, this study demonstrated for the first time that the glycolic extracts of C. pachystachya have promising chemical and biological characteristics for the development of a multifunctional cosmetic with antioxidant and tyrosinase-inhibition activities. (bvsalud.org)
Acidic3
  • Our manufactured Aluminium Oxide Acidic, a key player in this landscape, boasting unique attributes that elevate separation processes. (column-chromatography.com)
  • With a specific surface area of 150 +- 10% m2/g, Our Aluminium Oxide Acidic sets the stage for robust interactions with analytes, optimizing separation outcomes. (column-chromatography.com)
  • The inherently acidic nature of Aluminium Oxide Acidic facilitates the separation of acidic compounds with accuracy. (column-chromatography.com)