Sensitivity and specificity of rna detection. Medical search. Frequent questions

Since 2001, LabNet reference laboratories in Australia, Cote d'Ivoire, Netherlands, Turkey, Uganda, the UK, and the United States have been working to 1) determine IgM and RNA stability in DBS and OF samples and 2) optimize the methods for IgM antibody assay and protocols for RNA detection in DBS and OF samples ( 8--10 ). (cdc.gov)
This multisite clinical evaluation describes the validation of an improved sample preparation method for extraction-free RT-LAMP and reports clinical performance of four RT-LAMP assay formats for SARS-CoV-2 detection. (surrey.ac.uk)
An extraction-less validation protocol was carried out to determine performance characteristics of the assay in both synthetic SARS-CoV-2 RNA as well as clinical specimens. (medrxiv.org)
Feasibility of the assay and analytical sensitivity was first determined by testing a dilution series of synthetic SARS-CoV-2 RNA in two different solvents (water and AMIES VTM), revealing a high degree of linearity and robustness in fluorescence readouts. (medrxiv.org)
Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. (peerj.com)
A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. (peerj.com)
This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. (peerj.com)
To our knowledge, this is the first report of the detection of SARS-CoV-2 by real-time reverse transcription LAMP (RT-LAMP) assay with HNB. (peerj.com)
Cited in more than 40K publications to date, TaqMan Assays deliver the specificity, sensitivity, and reproducibility your research deserves-not to mention they're backed by our TaqMan Assay performance guarantee . (thermofisher.com)
We aimed to evaluate the LIAISON ® SARS-CoV-2 antigen assay (DiaSorin), comparing its performance to real-time polymerase chain reaction (RT-PCR) for the detection of SARS-CoV-2 RNA. (mdpi.com)
The sensitivity and specificity of the antigen assay were calculated for different cut-offs, with RT-PCR serving as the reference method. (mdpi.com)
In this thesis, a qPCR assay was developed for S. salar detection. (dcu.ie)
We show the applicability of this technique to three salmonid species, with the S. salar assay compared to qPCR as a detection/non-detection assay in samples from Ireland and Canada. (dcu.ie)
To facilitate this RPA-CRISPR-Cas assay as an on-site detection tool, the method was adapted for visualisation on a custom portable fluorometer and via lateral flow. (dcu.ie)
Both systems maintain the specificity and sensitivity of the original assay but enable simplified readouts without complex instrumentation. (dcu.ie)
Assay precision determined by testing 4 different log reduction (LR) levels in human RNA, using 2 operators and 8 runs for a total of 192 data points. (asuragen.com)
The QuantideX ® qPCR BCR-ABL minor Kit, an in vitro diagnostic (IVD) assay, enables ultra-sensitive detection of BCR-ABL1 minor fusion transcripts from whole blood specimens. (asuragen.com)
The detection of IgY in yolk samples by ELISA using streptavidin-biotin binding as part of the assay methodology requires some technology to neutralize high concentration of biotin on sample or more steps beyond delipidation to isolate the target protein. (preprints.org)
The sensitivity of the assay is very dependent upon the time of illness onset in which the specimen is collected. (testcatalog.org)
The lower LOD of this assay is 1 to 5 target copies/mcL of RNA extract for serum, urine, and spinal fluid, and 27 to 60 copies/mcL for EDTA whole blood. (testcatalog.org)

A method for the detection of coxsackievirus B3 RNA was developed using the polymerase chain reaction based on the amplification of a cDNA copy of the positive-strand viral RNA. (nih.gov)
The results show that the polymerase chain reaction is a useful means for detecting coxsackie viral RNA and its application should help in the evaluation of hypotheses concerning the infectious etiology of human myocarditis and idiopathic dilated cardiomyopathy. (nih.gov)
In addition, detection of viral RNA by reverse transcription--polymerase chain reaction (RT-PCR), usually in a throat swab or urine sample, and subsequent genotyping of strains is valuable for diagnosis and molecular epidemiology ( 2 ). (cdc.gov)
Nucleic acid amplifications tests (NAAT) such as multiplex reverse-transcriptase polymerase chain reaction (RT-PCR) remain the test of choice 3 for the detection of respiratory viruses due to their sensitivity, specificity, and time to virus detection. (medrxiv.org)
Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. (peerj.com)
ABSTRACT We evaluated the COBAS AMPLICOR polymerase chain reaction (PCR) based test for the detection of Mycobacterium tuberculosis complex in 866 respiratory and non-respiratory samples. (who.int)
AccuPower ® SARS-CoV-2 Real-Time RT-PCR Kit is an in vitro diagnostic kit that helps diagnose COVID-19 infections, detects SARS-CoV-2 (E gene and RdRp gene) RNA from a suspected infection patient's sample (such as sputum, nasopharyngeal swab, oropharyngeal swab) through Real-Time Polymerase chain reaction(PCR). (jurupro.com)
However, polymerase chain reaction (PCR) testing can be used to detect WNV RNA in serum, whole blood, and urine specimens from patients with recent WNV infection (ie, 3-5 days following infection) when specific antibodies to the virus are not yet present. (testcatalog.org)
For HCV RNA polymerase chain reaction (PCR) testing in adults, 4-6 mL of blood should be collected into an EDTA (purple-top) tube. (medscape.com)

The surging COVID19 pandemic has underlined the need for quick, sensitive, and high-throughput SARS-CoV-2 detection assays. (medrxiv.org)
And so, this requires particular types of molecular assays to detect RET fusions with a high degree of sensitivity and specificity. (reachmd.com)
These are next-generation sequencing assays and we're differentiating between DNA-based and RNA-based assays. (reachmd.com)
We know that RNA-based sequencing assays are able to identify novel fusion partners, able to identify functional transcripts, don't necessarily need to tie along all the introns. (reachmd.com)
In this setting, antigen rapid tests yielded an overall sensitivity of 83.3% (not significantly different between the four assays) while the use of automated antigen dosing would have spared 93.5% of asymptomatic inpatient screening PCRs. (frontiersin.org)
Applied Biosystems predesigned TaqMan Assays consist of target-specific primers and one or more probes optimized for specific applications, including gene expression, SNP genotyping, miRNA, mutation detection, and copy number variation analysis. (thermofisher.com)
First, we developed three specific ddPCR-based assays to detect EWS-FLI1 or EWS-ERG fusion transcripts, which naturally showed superior sensitivity to DNA detection on in vitro control samples. (elsevierpure.com)
These patient-specific assays show that although plasma tumor RNA can be detected in select newly diagnosed patients, positive results are low and statistically unreliable compared with ptDNA assays, which reproducibly detect robust positive results across most patients. (elsevierpure.com)
Here, we conclude that, even with optimized highly sensitive and specific assays, tumor DNA detection is superior to RNA detection in Ewing sarcoma patients. (elsevierpure.com)
AF-100S features long battery life, a small size, and an easy-to-use interface, making it ideal for use anywhere, and its high sensitivity and specificity ensure that patients receive accurate results with a wide range of assays. (whnt.com)
Analysis of clinical samples showed a good correlation between the INT-O and LTR-O assays (r = 0.8240), with an improvement of analytical sensitivity. (scienceopen.com)
Constant monitoring of genetic changes in the circulating influenza A(H1N1)pdm09 viruses is important for maintaining the sensitivity of molecular detection assays. (who.int)
RNA Mini Kit (Qiagen, Hilden, Germany) according to PCR assays are rapid, sensitive methods for detecting the the manufacturer's instructions. (who.int)
Qualitative tests only detect the presence of HCV RNA (specificity, approximately 98%) whereas the quantitative assays detect the viral load in IU/mL (specificity, approximately 98-99%) and provide adequate information that guides clinicians to manage and monitor treatment outcome. (medscape.com)

The kit provides high levels of specificity, sensitivity, and reproducibility. (qiagen.com)
Analytical sensitivity, repeatability, and reproducibility were also determined. (scienceopen.com)

These include superior sensitivity and specificity, as well as the ability to reveal dynamic changes in the biology of a patient's tumor over time. (lelezard.com)

Shortly after its identification, 2019_nCoV was renamed to the current consensus 2 term SARS-CoV-2 and the first complete SARS-CoV-2 RNA sequence was made public by the Chinese Centers for Disease Control and Prevention. (medrxiv.org)

Hepatitis C virus (HCV) is a single-stranded RNA virus that causes liver inflammation that can progress over time to advanced fibrosis, cirrhosis, and hepatocellular carcinoma (HCC) ( 1 - 3 ). (cdc.gov)
West Nile virus (WNV) is a mosquito-borne flavivirus (single-stranded RNA virus) that primarily infects birds but occasionally infects horses and humans. (testcatalog.org)

Conventional laboratory confirmation of suspected cases of measles and rubella is based on the detection of virus-specific immunoglobulin M (IgM) in a single serum sample collected soon after the onset of symptoms ( 2 ). (cdc.gov)
This work has provided data on sensitivity and specificity of OF and DBS samples compared with serum and also has identified logistic challenges in implementing alternative sampling techniques. (cdc.gov)
Identification of HCV RNA in the serum is also necessary to determine the magnitude of viremia and HCV genotype. (cdc.gov)
The results demonstrate that serum mixed with RNA protective solution and blotted on Whatman filter paper no 3 can be effectively used for diagnosis, serotyping and genotyping of DENVs. (lww.com)
Studies indicate that whole blood testing by PCR may provide higher sensitivity when testing patients with acute WNV disease (up to 87%) compared to serum, plasma, urine, and cerebrospinal fluid testing. (testcatalog.org)
5) Serum testing offers lower sensitivity (26%) but may be used when it is the only specimen type available. (testcatalog.org)
After an acute HCV infection, HCV RNA could be detectable in serum within 2 weeks following exposure. (medscape.com)

Previous studies have described reverse-transcription loop-mediated isothermal amplification (RTLAMP) for the rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in nasopharyngeal/oropharyngeal swab and saliva samples. (surrey.ac.uk)
The kit is based on the amplification and detection of specific BCR-ABL Mbcr p210 b2a2 or b3a2 transcripts, relative to ABL control gene expression, in total RNA. (qiagen.com)
In order to further reduce costs and enable detection by the naked eye, we used hydroxynaphthol blue (HNB) dye for the colorimetric detection of the amplification reaction. (peerj.com)
This offered the detection and quantitation of potential targets in highly processed or degraded samples where PCR amplification was not possible due to template crisis. (hindawi.com)
amplification and detection system for rou- tine diagnostic PCR. (who.int)
The demand for clinical signs or symptoms of pulmonary or sensitivity, specificity and speed of M. tu- extra-pulmonary TB) which had been sent berculosis detection led to the development to the TB laboratory at the Saudi-British of nucleic acid-based amplification tests to Laboratories for MTBC testing. (who.int)
To circumvent this, we developed an isothermal approach that couples Recombinase Polymerase Amplification to CRISPR-Cas12a detection as a route to a cost-effective biosensor device. (dcu.ie)
AmplifyRP ® XRT for ToBRFV is a rapid RNA amplification and detection platform designed for testing peppers and tomatoes for Tomato brown rugose fruit virus . (agdia.com)
AmplifyRP ® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. (agdia.com)
A highly sensitive technique, PCR provides a quick means for nucleic acid cloning, taking only a few hours, with minimal templates for detection and amplification of sequences. (labmanager.com)
But it also covers the steps of the overall process of pre-examination, examination and post-examination for the analysis of nucleic acid amplification method for the detection of SARS-CoV-2, and specifies the methods and comprehensive assessment indicators including accuracy, limit of detection, inclusivity, and specificity, etc. (iso.org)

NATs differ from other tests in that they detect genetic materials (RNA or DNA) rather than antigens or antibodies. (wikipedia.org)
Here we report the development of a direct RT-PCR SARS-CoV-2 detection method that can reliably detect minute quantities of SARS-CoV-2 gRNA in nasopharyngeal swab samples as well as the presence of human genomic DNA. (medrxiv.org)
To ensure highest sensitivity, the ipsogen BCR-ABL1 Mbcr Kit has been optimized to detect BCR-ABL Mbcr p210 b2a2 or b3a2 transcripts and the ABL control gene using prediluted plasmid standards and primers and probe mixes (see figures Accurate detection of BCR-ABL Mbcr standards. BCR-ABL Mbcr standards (F1–F5): 10 1 , 10 2 , 10 3 , 10 5 , and 10 6 copies/5 µl. "> Accurate detection of BCR-ABL Mbcr standards and Reliable detection of ABL plasmid standards. ABL standards (C1–C3): 10 3 ,10 4 , and 10 5 copies/5 µl. "> Reliable detection of ABL plasmid standards ). (qiagen.com)
Exai Bio today announced new data from a 3,300 subject study demonstrating that its novel RNA- and AI-based platform can detect multiple cancer types at the earliest stages using a single, standard blood sample. (lelezard.com)
These results demonstrate that Exai's RNA- and AI-based platform can detect stage I cancer at very high sensitivity across multiple cancers while also maintaining the high specificity which is required for real-world clinical utility," said Patrick Arensdorf, Chief Executive Officer of Exai Bio. (lelezard.com)
The study demonstrates successful detection of stage I breast and prostate cancers which, to date, have both been extremely challenging to detect at early stages using other blood-based approaches. (lelezard.com)
Anbio's LAMP Solution is a diagnostic technique to amplify and detect specific DNA or RNA sequences. (whnt.com)
First invented in 1983 by American biochemist Kary Mullis, PCR allows scientists to amplify and detect DNA and RNA sequences. (labmanager.com)
Q: What are the real-world implications of being able to detect BRAF V600E with this level of sensitivity and specificity? (afirma.com)
2017 to March 2018, there was circulation of both detect a single target in a very small concentration of DNA influenza A and B, with influenza A(H1N1)pdm09 again or RNA because it uses a fluorescent dye that binds to predominating in Viet Nam. (who.int)

Quantitative PCR (qPCR) expands the conventional PCR process flow and provides improved sensitivity and specificity for the detection and quantification of DNA sequences. (labmanager.com)

We know that the sensitivity of FISH is high, as is the specificity. (reachmd.com)
As a result, the sensitivity is more moderate to high, it does have high specificity though, you know, like FISH, really should only be used in rare circumstances. (reachmd.com)
Thanks to their high sensitivity, ranging from 73.9 to 89.5% for high viral load samples [10 5 -10 7 RNA copies/swab ( 3 )], and their overall specificity ( 4 , 5 ), antigen-detection rapid diagnostic tests have been integrated in several countries' testing strategies ( 6 - 10 ) 1 , 2 . (frontiersin.org)
Notably, Exai's platform maintained high sensitivity and specificity in both independent training and testing cohorts, highlighting the strength of its unique generative AI technology. (lelezard.com)
The results demonstrate that Exai's platform may close the performance gap of other blood-based approaches and deliver what patients and clinicians need: high cancer detection rates at early stages coupled with fewer false positives. (lelezard.com)
This is mainly because of this tumor's high degree of sensitivity to radiation, as well as the anatomical constraints for surgical access to the highly complex nasopharyngeal region. (medscape.com)
Dr. Godwin's laboratory, which has received funding over the years from the OVERRUN Ovarian Cancer Foundation and the Vicki Welsh Ovarian Cancer Fund, is dedicated to finding better, more reliable methods for the detection of this deadly disease, particularly high-grade serous carcinomas, the deadliest form of ovarian cancer that primarily originates in the fallopian tubes and spreads rapidly. (kucancercenter.org)
Currently, no ideal prostate cancer (PCa) diagnostic or prognostic test is available due to the lack of biomarkers with high sensitivity and specificity. (lu.se)
However, achieving high sensitivity and high specificity remains a challenge. (elsevierpure.com)
The d iagnosis of hepatitis C virus (HCV) infection is made by detection of molecular and serologic al markers, but the cost of these methodologies is quite high and blood sample collection requires trained personnel what it is difficult in remote areas or prese nting few resources. (fiocruz.br)
Isolation of high quality RNA also allows for downstream analysis of malaria transcripts. (tropmedres.ac)
Thus, qPCR can quantify the initial number of copies of the template DNA with high accuracy, sensitivity, and over a wide dynamic range. (labmanager.com)
The specificity and sensitivity of such tests are relatively high and are of crucial significance for the early diagnosis of infectious diseases. (iso.org)
The QuantideX qPCR BCR-ABL minor Kit (RUO) delivers high performance through unmatched sensitivity and optimized laboratory efficiency. (asuragen.com)
SARS-CoV-2 (E gene and RdRp gene) RNA can be detected with high sensitivity. (jurupro.com)
Bioneer's proprietary Dual-HotStart technology accomplishes high sensitivity and specificity. (jurupro.com)
It is clinically meaningful that this classifier has both high sensitivity and specificity. (afirma.com)
Sensitivity and specificity are key characteristics for diagnostic tools, and a high sensitivity is important Ribonucleic acid (RNA) extraction was conducted on a when the test is used to identify emerging infectious 140l aliquot of each sample using the QIAamp Viral diseases. (who.int)
These tests have very high sensitivity and specificity for HCV detection (sensitivity of greater than 99%, specificity of 99% in immunocompetent patients). (medscape.com)

Nucleic acid (DNA and RNA) strands with corresponding sequences stick together in pairwise chains, zipping up like Velcro tumbled in a clothes dryer. (wikipedia.org)
The Luna Probe One-Step RT-qPCR Kit (No ROX) is optimized for real-time quantitation of target RNA sequences using hydrolysis probes. (neb.com)

Following analytical performance using synthetic RNA, the limit of detection was determined at equal to or less than 1 SARS-CoV-2 copy/ul of sample in a commercially available sample panel that contains surrogate clinical samples with varying SARS-CoV-2 viral load. (medrxiv.org)
We then established a diagnostic algorithm by approaching median viral loads in target populations and evaluated the limit of detection of each test using the PCR cycle threshold values. (frontiersin.org)
The limit of detection (LODs) of Allplex, PowerChek, and Real-Q was determined by testing the transcribed RNA of SARS-CoV-2 E and the RNA of SARS-CoV Frankfurt1. (frontiersin.org)
Its detection limit is equivalent to that of commercial kits. (scienceopen.com)
Accuracy studies were performed by testing negative clinical specimens with whole viral genomic RNA for lineages 1 and 2 near the limit of detection (LOD) and yielded greater than or equal to 97% sensitivity and specificity. (testcatalog.org)

The BCR-ABL1 minor breakpoint (e1a2) constitutes a rare but distinct leukemic variant and accurate detection and quantitation of these fusion transcripts are paramount to improving outcomes for all chronic myeloid leukemia (CML) patients. (asuragen.com)
Rapid, sensitive and precise probe-based qPCR detection and quantitation of target RNA targets. (neb.com)
One-Step RT-qPCR provides a convenient and powerful method for RNA detection and quantitation. (neb.com)
In a single tube, RNA is first converted to cDNA by a reverse transcriptase, and then a DNA-dependent DNA polymerase amplifies the cDNA, enabling quantitation via qPCR. (neb.com)

All cardiac specimens with adequate RNA for study from coxsackie B3-infected mice contained detectable viral RNA, in contrast to none of control specimens from noninfected mice. (nih.gov)
In patients with myocarditis, one of five specimens contained detectable viral RNA, whereas none of 11 specimens from patients with idiopathic dilated cardiomyopathy or 21 myocardial specimens from patients with a wide variety of other cardiac disorders contained detectable coxsackie B3 viral RNA. (nih.gov)
Upon the detection of either type of the antigen preparation, the lowest concentration of the antigen detectable in buffer with pH 5.1 has been found to be approximately equal, amounting to ~10 -14 M. This value has been found to be similar upon the use of either type of molecular probes. (preprints.org)

Additionally, numerous non-coding RNAs (ncRNAs) can be detected in liquid biopsy. (diagenode.com)
Study the occurrence of 5-methylcytosine (5-mC) in RNA -- a well-known methylation modification found in various RNA molecules including tRNAs, rRNAs, mRNAs, and non-coding RNAs (ncRNAs). (epigentek.com)

DBS has been used for various epidemiologic studies for the detection of measles- and rubella-specific IgG and IgM antibodies and viral RNA ( 3--5 ). (cdc.gov)
Detection of genetic materials allows an early diagnosis of a disease because the detection of antigens and/or antibodies requires time for them to start appearing in the bloodstream. (wikipedia.org)
Statistical analysis can be used, namely total anti-HCV This study was conducted from De- All data were entered into a Microsoft antibodies, HCV-RNA levels, HCV cember 2012 to March 2013 at the Excel worksheet and statistical analysis genotype and recently HCV core anti- Viral Hepatitis Research Laboratory at was performed using SPSS for Win- gen (HCVcAg) levels ( 5 ). (who.int)

Kawang K, Naoudom U, Kowitdamrong E, Kerr SJ, Ruxrungtham K, Nilaratanakul V. Sensitivity and specificity of anti-double-stranded RNA immunofluorescence for universal detection of viral infection in respiratory specimens . (hivnat.org)
The correct diagnosis and monitoring of HIV-1 group O (HIV-O) infection are essential for appropriate patient management, for the prevention of mother-to-child transmission, and for the detection of dual HIV-M/HIV-O infections. (scienceopen.com)
A test for hepatitis C virus (HCV) RNA is essential to identify persons with active, or current, HCV infection. (cdc.gov)
Rapid ascertainment of HCV infection status with reflex testing to RNA is critical to identify persons eligible for treatment. (cdc.gov)
To determine baseline trends in testing for active HCV infection before the updated 2013 guidelines, we examined the frequency of and factors associated with the performance of an HCV RNA test subsequent to a positive antibody test among persons in these same 4 large integrated healthcare organizations from 2003 through 2010. (cdc.gov)
HCV-RNA remains the gold standard for diagnosis of active HCV infection but HCVcAg can be used where PCR is not available. (who.int)
Egypt has the compared HCVcAg with HCV RNA and can lead to increased HCV mor- highest prevalence of HCV infection in genotype 4 infected patients ( 9-11 ). (who.int)
2 ). HCV infection is often further pare HCV-RNA quantification with The institutional review board of the complicated by underlying hepatitis B HCVcAg presence and quantification National Hepatology and Tropical and Shistosoma mansoni co-infection. (who.int)
A positive result indicates the presence of West Nile virus RNA and is consistent with early infection. (testcatalog.org)
On the other hand, HCV RNA results can be positive with negative anti-HCV findings in cases of early stages of acute infection, false positive results, or chronic infection in an immunosuppressed individual. (medscape.com)

Most blood biomarkers to date lack the necessary sensitivity and specificity for early detection of ovarian cancer. (kucancercenter.org)
There is an unmet medical need to develop combinations of multiple biomarkers which may have higher accuracy in detection of PCa and stratification of aggressive and indolent cancer patients. (lu.se)
Epigenetic alterations found in liquid biopsies have shown utility as biomarkers for early detection, prognosis, monitoring, and evaluation of therapeutic response in many types of cancer patients. (diagenode.com)
Another study analyzed the methylation of specific genes in urine sediments from patients with prostate cancer and showed the sensitivity of DNA methylation biomarkers in distinguishing early and late prostate cancer ( Rodriguez-Casanova 2021). (diagenode.com)

Small extracellular vesicles, on the other hand, contain enriched subsets of biomolecules mirroring the tumor cells of origin, including certain proteins and RNAs. (kucancercenter.org)
Data demonstrated that methylation profiling of CTCs represents a promising non-invasive approach for tumor detection. (diagenode.com)
The detection of tumor-specific nucleic acids from blood increasingly is being used as a method of liquid biopsy and minimal residual disease detection. (elsevierpure.com)
Here, we perform a direct comparison of two droplet digital PCR (ddPCR)-based detection methods, circulating plasma tumor RNA and circulating plasma tumor DNA (ptDNA), in blood samples from newly diagnosed Ewing sarcoma patients. (elsevierpure.com)
Furthermore, the unique disease biology of Ewing sarcoma enabled us to show that most cell-free RNA is not tumor-derived, although cell-free-DNA burden is affected strongly by tumor-derived DNA burden. (elsevierpure.com)

This kit includes lyophilized reaction pellets containing the necessary reagents to amplify ToBRFV RNA and an endogenous RNA control at a single operating temperature (42 °C). (agdia.com)

This large, rigorously designed study represents a significant achievement in the field of early cancer detection," stated Lee Schwartzberg, M.D., Chief, Medical Oncology and Hematology at the Renown Health-Pennington Cancer Institute. (lelezard.com)
In addition, Septin 9 methylation observed in cfDNAs may be a sensitive biomarker for early colorectal cancer detection. (diagenode.com)

Recent studies have shown that the detection of FCoV by reverse transcriptase quantitative polymer chain reaction (RT-qPCR), using template RNA, can provide further support for a diagnosis of effusive FIP, with sensitivity of ~ 65-89% and specificity of ~96-100% when testing effusions. (vin.com)
This may often involve the simultaneous detection of multiple targets in a single reaction well, using different pairs of primers for each target, with various flurophores or channels of colors available for use as probes. (labmanager.com)

single-stranded probe or primer molecules capture DNA or RNA target molecules of complementary strands. (wikipedia.org)
Investigate N6-methyladenosine (m6A) -- the most common and abundant methylation modification in RNA molecules present in eukaryotes. (epigentek.com)

Previously, Exai has presented non-small lung cancer data at the American Association for Cancer Research (AACR) meeting 2023, breast cancer early detection and screening data at the San Antonio Breast Cancer Symposium (SABCS) 2022, monitoring and molecular residual disease detection data in breast cancer at SABCS 2021, and colorectal cancer early detection and screening data at the 2022 European Society for Medical Oncology (ESMO) meeting. (lelezard.com)
Exai's universal platform can be used across multiple cancer care settings such as screening and early detection, monitoring, molecular residual disease and therapy selection. (lelezard.com)
Building on the simple workflow and best-in-class sensitivity established with the FDA-cleared QuantideX ® qPCR BCR-ABL IS Kit, the minor Kit allows labs and clinical researchers to examine the biology of disease for this very rare but distinct leukemic variant with unprecedented ease. (asuragen.com)
In the third paper, we investigated expression profiles of microRNA (miRNA) and small nucleolar RNA (snoRNA) in whole blood of individuals with TB disease, with and without HIV, and uninfected controls (total n=40). (lu.se)
Variants with the potential for increased transmissibility, more severe disease, diagnostic detection failures, or reduced response to available treatments and/or vaccines are tracked as Variants of Concern and are commonly referred to by their WHO-designated Greek alphabet label or their Pango lineage number. (msdmanuals.com)

According to the CDC Interim Guidelines for Collecting and Handling of Clinical Specimens for COVID-19 Testing, nasopharyngeal swabs (NPS) are the preferred method to obtain biological samples for respiratory virus detection. (medrxiv.org)

Even when present at lower levels, which is often the case when cancer is in its earliest phases, small extracellular vesicles serve as a promising biomarker for early detection," Dr. Godwin said. (kucancercenter.org)

Specificity was evaluated with 24 other respiratory virus-positive samples. (frontiersin.org)

Identification of SARS-CoV-2 positive and negative samples by end-point NextGenPCR RT-PCR is highly accurate as compared to a reference qPCR method on extracted RNA and, owing to its superior speed, is shown to be suitable for large-scale screening purposes where time to detection is a crucial factor. (medrxiv.org)
To do this, 304 samples collected from an active case detection program in Kayin state, Myanmar were compared using uPCR and RT-qPCR. (tropmedres.ac)
The QuantideX ® qPCR BCR-ABL minor Kit (RUO) is a clinical research tool enabling ultra-sensitive and precise detection of BCR-ABL1 minor fusion transcripts (e1a2) from whole blood specimens. (asuragen.com)
The QuantideX qPCR BCR-ABL minor Kit marries improved efficiency with unprecedented sensitivity, empowering labs to assess the deepest molecular responses in patients harboring the minor breakpoint with the ease-of-use they've come to expect from Asuragen. (asuragen.com)
This visible dye does not overlap spectrally with fluorophores commonly used in qPCR and does not interfere with real-time detection. (neb.com)

The first step is reverse transcription of total RNA in a sample into cDNA. (qiagen.com)

The updated algorithm features a specific sequence of tests to provide maximal sensitivity, specificity, and accuracy for HIV detection. (hivguidelines.org)

The emergence of SARS-CoV-2 has underlined the need for rapid and easy-to-implement diagnostic nucleic acid detection methods. (medrxiv.org)
I've outlined five different types of methods for RET detections, and I want to walk through both the pros and cons of each testing modality and ultimately whether it should be used or not. (reachmd.com)
Standard of Care methods to diagnose, or assess the treatment response of LM (Clinical Evaluation, MRI and Cytology) have limited sensitivity and specificity. (stanford.edu)
It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions. (agdia.com)
However, mutations performed using both standard molecular methods (con- in the viral genome that generate novel variants cause the ventional RT-PCR and real-time RT-PCR), employing sensitivity of these molecular tests to decrease and may specific primer and probe sets targeted to the matrix, hae- lead to false-negative results. (who.int)

The messenger RNA (mRNA) expression signature of the five genes was determined in 144 PCa and benign prostate specimens from. (lu.se)
Evidence has shown that RNA cytosine methylation affects various biological processes, such as RNA stability and mRNA translation. (epigentek.com)

Developing non-invasive and highly specific blood-based tests for pre-symptomatic screening and early detection of ovarian cancer is therefore crucial. (kucancercenter.org)
The early detection of cancer is of critical importance because survival rates markedly improve when the oral lesion is identified at an early stage. (medscape.com)
LMICs in the context of cancer control, in the number of doses for more effectiveness of cancer prevention and conducts clinical and screening trials, affordable and logistically feasible pro- early detection interventions to guide and conducts implementation and health grammes. (who.int)

Despite their limited sensitivity, SARS-CoV-2 antigen rapid diagnostic tests are vital tools in the fight against viral spread. (mdpi.com)

In this study we describe a highly efficient direct reverse transcriptase PCR (RT-PCR) protocol using our proprietary NextGenPCR ultra-fast thermocycler for the detection of SARS-CoV-2 genomic targets in NPS that does not require RNA extraction. (medrxiv.org)

and 4) a NAT for HCV RNA is recommended for perinatally exposed infants and children aged 7-17 months who previously have not been tested, and a hepatitis C virus antibody (anti-HCV) test followed by a reflex NAT for HCV RNA (when anti-HCV is reactive) is recommended for perinatally exposed children aged ≥18 months who previously have not been tested. (cdc.gov)

The methylation patterns may change with age and that may impact the sensitivity of detection, which is something that they didn't see in this particular study. (medscape.com)
Compare and choose the right RNA methylation kits for you. (epigentek.com)
Expand upon your understanding of RNA methylation with the help of EpiGentek's Global RNA Methylation Kits. (epigentek.com)
From directly quantifying the "fifth RNA base" N6-Methyladenosine (m6A) to measuring 5-mC RNA, our kits make it easy for you to determine how RNA methylation influences your research project. (epigentek.com)
Known as the "fifth RNA base", m6A RNA modifications are reversible, highly dynamic, and likely play important biological roles comparable to DNA methylation and histone modification. (epigentek.com)
Schematic procedure for the EpiQuik m6A RNA Methylation Quantification Kit (Cat# P-9005). (epigentek.com)

Des échantillons sériques prélevés sur 80 personnes suspectes d'être positives au VHC ont été testés simultanément pour la charge d'ARN du VHC au moyen de l'amplification en chaîne par polymérase en temps réel (RT-PCR) et pour les concentrations de l'antigène de la nucléocapside par ELISA. (who.int)

We assessed trends in HCV RNA testing following a positive HCV antibody result among persons in 4 large healthcare organizations. (cdc.gov)
Data collected from adults with ≥2 clinical encounters during 2003–2010 were analyzed to determine the frequency of, interval between, and factors associated with having an RNA test after a first positive HCV antibody test. (cdc.gov)
From 2003–2010, 5860 persons had a positive antibody test, of whom 3570 (60.9%) had a follow-up RNA test. (cdc.gov)
30 000 (by census geocode) were less likely to have had a follow-up RNA test done within 6 months of a positive antibody test. (cdc.gov)
Less than two-thirds of persons with a positive HCV antibody test had a follow-up RNA test. (cdc.gov)

The kit provides reagents optimized for reliable and sensitive detection and quantification of BCR-ABL Mbcr b2a2 or b3a2 transcripts, relative to ABL control gene expression, in total RNA. (qiagen.com)

The blots were dried and stored at 4°C and 28°C and tested for presence of dengue RNA, serotypes and genotypes after 7 days of incubation. (lww.com)

The Luna WarmStart RT Enzyme Mix is supplied at 20X concentration and contains Luna WarmStart Reverse Transcriptase as well as Murine RNase Inhibitor to aid in preventing RNA degradation (see also template preparation in product manual). (neb.com)

Accurate laboratory results for detection of IgM and viral RNA are dependent on proper collection, processing, shipment, and storage of clinical samples and use of accurate tests performed by a proficient laboratory. (cdc.gov)

The loss of 5-mC in vault RNAs may cause aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. (epigentek.com)

In the independent testing cohort, overall stage I sensitivity across eight cancer types was 87% at 95% specificity. (lelezard.com)

The sensitivity of the technique was established at approximately 1 to 100 plaque-forming units of virus per gram of tissue, and the specificity was established as limited to the coxsackievirus B3 serotype among nine viruses tested. (nih.gov)
Influenza detection was genetic material of influenza viruses. (who.int)

Bio-Rad's CFX Opus Real-Time PCR Systems utilize solid-state light-emitting diodes (LEDs) for fluorescence excitation and photodiodes for detection. (labmanager.com)

This new platform uniquely enables detection of low abundant membrane proteins, even in the background of complex plasma proteins. (exosome-rna.com)
The RB synthesizes its own DNA, RNA, and proteins but requires energy in the form of adenosine triphosphate (ATP) from the host cell. (medscape.com)

In addition to the MCED data, Exai's abstract entitled " Consensus molecular subtypes and orphan non-coding RNAs in colorectal cancer " was published in the 2023 ASCO Annual Meeting proceedings. (lelezard.com)
These data build upon Exai's previously published subtyping data in breast cancer, demonstrating that oncRNAs are also predictive of detection of colorectal cancer subtypes. (lelezard.com)

We were particularly interested in the BRAF classifier's sensitivity and specificity for this mutation. (afirma.com)

Anatomy11

Organisms12

Diseases6

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment76

Psychiatry and Psychology3

Phenomena and Processes65

Disciplines and Occupations5

Technology, Industry, Agriculture1

Information Science9

Health Care15

Assay20

Polymerase Chain Re9

Assays14

Reproducibility2

Superior sensitivity1

SARS-CoV-2 RNA1

Single-stranded RNA virus2

Serum7

Amplification11

Detect10

Quantification of DNA Sequences1

High19

Sequences2

Limit5

Quantitation4

Detectable3

NcRNAs2

Antibodies3

Infection10

Biomarkers4

Tumor5

Amplify1

Cancer detection2

Reaction2

Molecules2

Disease5

Clinical specimens1

Vesicles1

Respiratory1

QPCR5

CDNA1

Accuracy1

Methods5

MRNA2

Early detection3

Antigen rapid1

Genomic1

Hepatitis C vir1

Methylation6

ELISA1

Antibody5

Reagents1

Dengue1

Reverse transcriptase1

Accurate1

Aberrant1

Cohort1

Viruses2

Fluorescence1

Proteins2

Colorectal cancer2

Mutation1