• AdnaTest ProstateCancerSelect enables the immunomagnetic enrichment of tumor cells via epithelial and tumor associated antigens. (qiagen.com)
  • The aim of this thesis was to realize single cell isolation by means of automated immunomagnetic bead enrichment and antibody-based fluorescence detection in a chip-based microfluidic system and the molecular biological characterization of these cells to assay tumor features from liquid biopsy. (fraunhofer.de)
  • Therefore, this work compares manual and automated immunomagnetic enrichment in terms of cell recovery in dependency of cell line and input number, bead characteristics, biofunctionaliza-tion protocol and blood cell contamination focusing on head and neck squamous cell carcinoma (HNSCC) and metastatic breast cancer. (fraunhofer.de)
  • In a prototype enrichment device (IsoMAG), we established an isolation protocol with EpCAM-targeting 1 µm magnetic beads for automated enrichment tested on different epithelial cancer cell lines. (fraunhofer.de)
  • In the microfluidic benchtop platform CTCelect, we characterized each process step with two different carcinoma cell lines (MCF-7, SCL-1) demon-strating up to 87 Percent enrichment, 73 percent optical detection and dispensing efficiency. (fraunhofer.de)
  • CTCs are present in the bloodstream at a very low concentration, thus their detection and characterization require highly sensitive and specific methods, which consist of a combination of enrichment (isolation) and detection (identification) strategies ( Table 1 ) and both steps are essential components of the identification process ( 19 ). (amegroups.org)
  • Previously defined strategies for CTC detection, isolation, and enrichment are based on procedures including density, immunomagnetic, size-exclusion and/or circulation manipulation in microfluidic devices (4-8). (biotechnologyconsultinggroup.com)
  • Reverse transcription results in cDNA, which is subsequently used as template for preamplification and tumor cell detection and characterization by qRT-PCR. (qiagen.com)
  • Isolation and characterization of a unique strain of Rickettsia parkeri associated with the hard tick Dermacentor parumapertus Neumann in the western United States. (cdc.gov)
  • Bacteriophages against Vibrio coralliilyticus and Vibrio tubiashii: Isolation, characterization and remediation of larval oyster mortalities. (usda.gov)
  • Quantification and characterization of exosomal proteins is then performed using appropriate detection antibodies against exosome-associated antigens, which can be either generic or cell / tissue specific exosomes. (immpact-international.org)
  • Isolation and characterization of CD34 + blast-derived exosomes in acute myeloid leukemia. (immpact-international.org)
  • A fast capillary-channeled polymer (C-CP) fiber spin-down tip method for the isolation of plant-derived extracellular vesicles (PDEVs) from 20 widespread fruit and vegetable sources. (lefnec.com)
  • To explore the heterogeneity of CTCs, a number of antibody-based and transcript-based assays have been developed for detection of a broader range of melanoma CTCs. (nature.com)
  • The purpose of this review is to provide a complete update of the various analytical methods for virus detection, such as molecular, immunological, sensor-based and other detection assays, along with the advantages and limitations of these strategies. (frontiersin.org)
  • We highlighted the bottlenecks of microfluidic cell isolation in dependency of bead-binding on the cell surface and implemented a gating function for the cytometer subunit to selectively dispense cells instead of autofluorescent objects. (fraunhofer.de)
  • It provides a comprehensive list of immunomagnetic bead products conjugated with different coating materials and functional groups in multiple sizes for research and industrial prospect development. (selfgrowth.com)
  • The defense of his dissertation on "Automated Isolation of Circulating Tumor Cells and their Molecular Biological Analysis" took place on January 30, 2023 at the Johannes Gutenberg University Mainz. (fraunhofer.de)
  • The application of molecular techniques in food is large, several authors comment on the detection and quantification of strains of pathogenic microorganisms present in foods and the presence of toxins and waste allergens not related to specific goods in production lines. (peertechzpublications.com)
  • Molecular techniques have facilitated the identification, genotyping, and monitoring of food contamination sources, identification of genetically modified organisms, and use as food detection methods to avoid falsification through genetic markers. (peertechzpublications.com)
  • Nonetheless the small number of CTCs compared to billions of blood cells in a patient sample requires sensitive methods to obtain reproducible tumor cell recovery rates. (fraunhofer.de)
  • The large heterogeneity of melanoma CTCs has hindered their detection and clinical application. (nature.com)
  • The heterogeneous expression of markers, together with their rare frequency (1-10 CTCs in 8 mL of blood) are the main intrinsic factors hampering CTC isolation and limiting in-depth study of these cells in patients with melanoma. (nature.com)
  • In the last decade, the detection of disseminated tumor cells (DTCs) in the bone marrow and circulating tumor cells (CTCs) in the blood have demonstrated to provide useful information for the clinical management of BC by predicting treatment benefit earlier than traditional imaging methods. (amegroups.org)
  • The aim of this review is to describe the current state of the art on CTCs detection and clinical use, the evidence to demonstrate their clinical validity, and their potential impact for both future clinical trial design and, decision-making process in our daily practice. (amegroups.org)
  • in this scenario, careful consideration must be given to the isolation approach, whereas methods that discriminate against certain subpopulations may result in the exclusion of CTCs that carry biological relevance. (oaepublish.com)
  • Herein, we review the history and the current status of CTC detection, discuss the prognostic, predictive, and diagnostic capabilities of these technologies, and describe application of CTCs as models for the study of tumor biology. (amegroups.org)
  • Unlike other detection methods, Pathatrix allows the entire pre-enriched sample or large pooled samples to be recirculated over antibody-coated paramagnetic beads. (wikipedia.org)
  • The combination of highly specific immunomagnetic cell selection system (using the optimized antibody combination, see "A CTC captured by three antibodies coupled to magnetic beads ") and highly sensitive RT-PCR technology (using a combination of mRNA tumor markers) provides the highest degrees of analytical specificity and sensitivity. (qiagen.com)
  • AdnaTest ProstateCancerPanel AR-V7 contains Oligo (dT)25 Beads for the isolation of mRNA from the lysate of pre-enriched tumor cells. (qiagen.com)
  • These findings indicate that LMS with WGA coupled to magnetic beads could be an efficient isolation step in the analysis of water sample concentrates for T. gondii oocysts, with detection either by microscopy or by qPCR. (usda.gov)
  • In general, the most known and used methods are Western Blots (WB), Enzyme-Linked Immune Sorbent Assay (ELISA), Lateral-Flow Assay (LFA), and Dot-Blot Assay (DBA) and have been applied in the detection of carcinogenic compounds, pathogenic and allergenic microorganisms using antibodies [8]. (peertechzpublications.com)
  • Hence, this review reports possible methods and strategies for the fast and reliable detection of ZIKV with particular emphasis on current updates, knowledge, and new hypotheses that might be helpful for medical professionals in poor and developing countries that urgently need to address this problem. (frontiersin.org)
  • This review summarizes the currently available methods employed for the rapid detection of ZIKV and suggests an innovative approach involving the application of a liposome-based hypothesis for the development of new strategies for ZIKV detection and their use as effective biomedicinal tools. (frontiersin.org)
  • Considering the calamity of ZIKV infection, there is an urgent need to develop rapid detection methods for ZIKV along with DENV, which shares common clinical symptoms with ZIKV. (frontiersin.org)
  • In this review article, we aimed to present contemporary methods and techniques for the sampling, isolation, and analysis of single cells and to give an overview of the current scientific literature about CRC at the single-cell level. (por-journal.com)
  • The simple and sensitive signal amplification strategy exhibits great potential for early clinic diagnosis by precise protein profiling of sEVs. (bvsalud.org)
  • In this review article we aimed to emphasize the importance of single-cell analyses by presenting tumor heterogeneity and the limitations of conventional investigational approaches, followed by an overview of the whole single-cell analytic workflow from sample isolation to amplification, sequencing and bioinformatic analysis and a review of recent literature regarding the single-cell analysis of colorectal cancers. (por-journal.com)
  • Moreover, neither isolation approach is readily compatible with routine clinical workflows, which is still an underappreciated aspect in studies attempting to harness the potential of EVs for medical diagnostics. (biomedcentral.com)
  • Aiming to provide proof-of-concept evidence and methodology for the potential role of serum-derived EVs as biomarkers in leukemic versus healthy samples in this study, we hope to pave the way for future detection of promising biomarkers for imminent disease progression and the identification of potential targets to be used in a therapeutic strategy. (nature.com)
  • Preclinical research on non-invasive biomarkers for the detection and monitoring of a number of pathological conditions (inflammation, cancer, neurodegeneration, etc. (immpact-international.org)
  • The incorporation of nanomaterials provides highly sensitive sensing devices allowing the efficient detection of pesticides. (scirp.org)
  • Addition-ally, the platform was compared to commercial CTC isolation technologies to highlight advantages and limitations of CTCelect. (fraunhofer.de)
  • Here, we addressed central aspects of EV immunoaffinity isolation from simple and complex matrices, such as plasma. (biomedcentral.com)
  • Although these biosensors are currently among the less popular tools for human disease detection, they have become useful tools for the screening and detection of pathogenic bacteria, fungi, and viruses because of their versatile advantageous features compared to other sensing devices. (frontiersin.org)
  • For 12 enrichments of tumor cells from whole blood and subsequent detection of prostate cancer-associated gene expression including AR-V7 expression. (qiagen.com)
  • This allows sensitive analysis of prostate cancer-associated gene expression including AR-V7 detection in immunomagnetically enriched tumor cells by reverse transcription and PCR. (qiagen.com)
  • The differential clinical value carried within each distinct EV subset highlights the advantages of selective isolation. (biomedcentral.com)
  • This review describes the current state of the art on CTC detection strategies, the evidence to demonstrate their clinical validity, and their potential impact for both future clinical trial design and, decision-making process in our daily practice. (amegroups.org)
  • The results of two large phase III clinical trials (STAMPEDE trial and CHAARTED trial) support the use of taxane chemotherapy in combination with standard androgen deprivation therapy (ADT) in patients with hormone-sensitive metastatic PC [ 3 , 4 ] . (oaepublish.com)
  • This EV isolation protocol facilitated the extraction of clinically useful information from plasma. (biomedcentral.com)
  • Its Absolute Mag™ platform is dedicated to developing magnetic microsphere for life science applications, such as immunoprecipitation, cell isolation, RNA/DNA extraction, and protein purification. (selfgrowth.com)
  • AdnaTest ProstateCancerPanel AR-V7 is a highly specific immunomagnetic cell-selection system for enriching circulating tumor cells from peripheral blood. (qiagen.com)
  • This cell isolation kit targets non-B cells for removal based on specific surface antigens. (stemcell.com)
  • Pathatrix is a high volume recirculating immuno magnetic-capture system developed by Thermo Fisher Scientific (and supplier parts by Life Technologies) for the detection of pathogens in food and environmental samples. (wikipedia.org)
  • Until recently, Salmonella enterica serotype Enteritidis has from imported poultry products, including imported broiler remained sensitive to most antibiotics. (cdc.gov)
  • Mitochondrial-related gene expression changes are sensitive to agonal-pH state: implications for brain disorders. (ucdavis.edu)
  • This situation has created an alarming public health emergency of international concern requiring the detection of this life-threatening viral candidate due to increased cases of newborn microcephaly associated with ZIKV infection. (frontiersin.org)
  • CTC detection using our multimarker approach was associated with shorter overall and progression-free survival. (nature.com)
  • The Pathatrix is a microbial detection system that allows for the entire sample to be analyzed. (wikipedia.org)
  • Plasma-derived EVs were captured and detected using this straightforward protocol, sequentially combining isolation and staining of specific surface markers, such as CD9 or CD41. (biomedcentral.com)
  • 2005. Development of a species-specific and sensitive detection assay for Phytophthora infestans and its application for monitoring of inoculum in tubers and soil . (hutton.ac.uk)
  • Optical, electrochemical and piezoelectric biosensors have been reported based on the detection method. (scirp.org)
  • Against this background, the Fraunhofer Institute for Microengineering and Microsystems has recognized the unmet need of standardized CTC isolation and developed automated microfluidic platforms to enrich, detect and isolate single tumor cells from 7.5 mL whole blood. (fraunhofer.de)
  • 40 to 56.7 percent of cells were recovered after complete isolation from 7.5 mL untreated whole blood. (fraunhofer.de)
  • This kit isolates untouched and highly purified B-CLL cells directly from human whole blood by fully immunomagnetic negative selection. (stemcell.com)
  • Stiefel's thesis deals with detecting tumor features based on the automated isolation of circulating tumor cells from liquid biopsies and thus contributes significantly to establishing a platform technology of Fraunhofer IMM in applied research of liquid biopsy. (fraunhofer.de)
  • Key Features: Sensitive and accurate. (immpact-international.org)
  • The AdnaTest's successful CTC detection is based on the Combination of Combinations Principle (COCP). (qiagen.com)
  • They have shown a wide range of application prospects in the fields of sensor detection, disease diagnosis, gene therapy vectors, and targeted drug delivery. (selfgrowth.com)
  • Application of LMS integrated with qPCR enabled detection of a single oocyst/ml in dirty water sample concentrate. (usda.gov)
  • Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. (immpact-international.org)
  • Since blood plasma circulates through the whole body, it is often the biofluid of choice for biomarker detection in EVs. (biomedcentral.com)
  • This review gives an overview of recent advances and new trends in biosensors for pesticide detection. (scirp.org)
  • Although the improvements in BC detection and adjuvant treatments led to a significant decrease in BC-related deaths in the last two decades, about 30% of women initially diagnosed with early-stage cancer eventually develop metastatic disease. (amegroups.org)
  • Multiplexed digital transcript detection data was generated using the Nanostring nCounter platform and evaluated through a dedicated bioinformatics pipeline. (biomedcentral.com)