Sensitive cycloheximide. Medical search. Frequent questions

Indeed, DNA isolated from uninduced mesenchyme showed progressive degradation, a process that was prevented by treatment with actinomycin-D or cycloheximide and by buffering intracellular calcium. (rupress.org)
This dexamethasone-dependent repression involved a reduced COX-2 mRNA half-life, was blocked by actinomycin D or cycloheximide, and was antagonized by the steroid antagonist RU38486. (mdc-berlin.de)

At prolonged incubation (14 days, 30°C), a fungal culture on BD Difco dehydrated culture media Sabouraud brain heart infusion agar base (with chloramphenicol and cycloheximide) ( https://www.bd.com ) showed flat, floccose to powdery, whitish growth. (cdc.gov)

Cycloheximide is a protein synthesis inhibitor in eukaryotic cells. (goldbio.com)
Inhibition of nascent protein synthesis with cycloheximide prevents AZC-induced protein aggregation and abrogates ROS generation confirming that aggregate formation causes ROS production. (manchester.ac.uk)
Nuclear movement and flagellar root contraction and extension are not sensitive to inhibitors of protein synthesis (cycloheximide), or drugs that influence either microtubules (colchicine) or actin-based microfilaments (cytochalasin D). Detergent-extracted cell models contract and extend their flagellar roots and move their nuclei in response to alterations of free calcium levels in the medium. (rupress.org)
Estrogen modulation of HIF-1 and subsequent effects on endothelial cells is dependent on the Akt/PI3K pathway and protein synthesis as validated by the use of the inhibitors wortmannin and cycloheximide which abrogated estrogen's effects respectively. (biomedcentral.com)
By using this sensitive assay, the kinetics of oestrogen-induced elaboration of this specific protein in immature chicks was investigated. (iisc.ac.in)
α-Amanitin and cycloheximide administered along with or shortly after the sex steroid severely curtailed the protein elaboration. (iisc.ac.in)

HSPA5 mRNA was induced by 3 μM BaP in an AD-sensitive manner, but this response was unaffected by the presence of heavy metals. (bioone.org)

Sabouraud's dextrose agar ) at room temperature or 37 °C. Cryptococcus neoformans are sensitive to cycloheximide so media containing cycloheximide should be avoided. (microbeonline.com)

Cycloheximide in this formulation inhibits saprophytic fungi, while Nalidixic Acid is used to suppress the growth of Gram-negative organisms. (alphabiosciences.com)

The induction response was sensitive to transcriptional and translational inhibition by actinomycin D (AD) and cyclohexamide, respectively. (bioone.org)
Myelomas are antibody-secreting tumor cells derived from terminally differentiated B lymphocytes, and previous work from our laboratory showed that murine SP2/0 myeloma cells and derived B-cell hybridomas were highly sensitive to apoptosis induction by a block of gene expression (cycloheximide). (aacrjournals.org)
In human pulmonary A549 cells, interleukin-1beta (IL-1beta) increases prostaglandin E2 (PGE2) synthesis via dexamethasone-sensitive induction of COX-2. (mdc-berlin.de)

Bacitrac99 is the most pure form of bacitracin available, and should be used for the most sensitive applications and on the most sensitive cell lines since any impurities that might cause inhibition of resistant cell lines have been removed. (goldbio.com)

Yeast mutants lacking TSA1 are sensitive to misfolding caused by exposure to the proline analogue azetidine-2-carboxylic acid (AZC). (manchester.ac.uk)
All mutants tested were cross-resistant to other benzimidazoles but not to cycloheximide or emetine. (ox.ac.uk)
MBC resistance of benB and benD mutants was expressed in plasmodia, but benA and benC mutant plasmodia were MBC sensitive, suggesting that benA and benC encode myxamoeba-specific products. (ox.ac.uk)
Clb2-LacZ and Visual Screen Temperature-sensitive mutants were patched onto YPD plates BIBR 953 and allowed to grow overnight at 23°C. They were then replica plated to YPD and placed at 37°C for 4 h replica plated again to Whatman filters (VWR San Francisco CA) on YPD plates that contained 1 μg/ml α-factor and returned to 37°C for 5 h. (bibf1120.com)

A biochemical correlate of apoptosis is the transcriptional activation of a calcium-sensitive endonuclease. (rupress.org)

Cycloheximide can be used to study cellular processes and inhibit yeast growth. (goldbio.com)

The activity of SMAL was sensitive to pronase and heating at 100(DEGREES)C for 30 minutes but insensitive to RNase. (etsu.edu)

An IFN-sensitive luciferase bioassay was utilized to determine IFN focus [20] and cyclohexamide-treated TNF-α-delicate L929 cells had been utilized to determine TNF-α creation [20]. (biobender.com)

Previously, it has been demonstrated that honeybees are sensitive to one aspect of the US magnitude, the US duration, in a visual conditioning paradigm ( Menzel, 1968 ). (jneurosci.org)

Mark A.D, et al 2010).Thesekind of studies depends on the chemical shift pattern of FNMR as this method isvery sensitive to change in its environment due to the presence of fluorine 19,as well as the existing weak Vander Waal force of bond as well as the presenceof the local electrostatic field. (relatewithkatypark.com)

Many diagnostic laboratories have replaced this test with a more sensitive cryptococcal latex agglutination test . (microbeonline.com)
En utilisant l'épreuve de fixation du complément comme méthode de référence pour les tests de confirmation, on a constaté que le test au Rivanol était plus sensible mais moins spécifique que l'épreuve d'agglutination en tube pour le dépistage de la brucellose. (who.int)