Self renewal and differentiation. Medical search. Frequent questions

However, the possible differences in proliferation and differentiation capabilities among independently-derived human embryonic stem cells (hESCs) are not well known because of insufficient characterization. (biomedcentral.com)
The regulations of NPCs' differentiation and proliferation by the Ras-ERK pathway were also shown by the rescues of the effects of bFGF depletion, neuronal differentiation, and antiproliferation by epidermal growth factor. (elsevierpure.com)
In summary, Sur8 is an antidifferentiation factor that stimulates proliferation for maintenance of self-renewal in NPCs via modulation of the Ras-ERK pathway. (elsevierpure.com)
This includes the analysis of genes in germline stem cell proliferation and differentiation. (ncl.ac.uk)
Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
TWS 119 enhances the proliferation and survival of γδ T-cells, and induces differentiation of CD8+ T cells towards a stem cell-like memory T cell phenotype at 2.5 μM. (tocris.com)
Compounds for NSC Proliferation and Renewal " has 8 results in Products. (rndsystems.com)
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. (nih.gov)
Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)
Pax6 serves as a regulator in the coordination and pattern formation required for differentiation and proliferation to successfully take place, ensuring that the processes of neurogenesis and oculogenesis are carried out successfully. (biolegend.com)
STAT3 protein belongs to a group of intracellular transcription factors that mediate a variety of functions such as cellular differentiation, proliferation, and apoptosis. (biolegend.com)
The Wnt signaling pathway promotes the growth and division (proliferation) of cells and helps determine the specialized functions a cell will have (differentiation). (medlineplus.gov)
These genes increase the proliferation and differentiation of cells associated with the hair follicle matrix. (medlineplus.gov)
Hematopoiesis, in particular the genetic control of hematopoietic cell proliferation and differentiation is also studied in human cell cultures and transgenic mouse model systems. (lu.se)
A homeodomain protein and transcription regulator that functions in BLASTOCYST INNER CELL MASS and EMBRYONIC STEM CELL proliferation and CELL SELF RENEWAL. (bvsalud.org)
Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)

They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
Hematopoietic stem/progenitor cells (HSPC) are known to be functionally diverse in terms of their self-renewal potential and lineage output. (lu.se)

Promotes differentiation of cardiomyocytes from human pluripotent stem cells (PSCs) that have been induced to mesoderm by addition of Activin A and/or BMP4 (Ren et al. (stemcell.com)
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids. (tocris.com)

In stratified epithelia proliferative basal cells adherent to the underlying basement membrane undergo cell cycle arrest then outward migration and terminal differentiation. (stanford.edu)
These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
Cancer stem cells are a selective clonal subset of tumour cells that have avoided various cell regulatory mechanisms, including terminal differentiation, and yet have retained the self-renewal properties and proliferative potential of adult stem cells. (who.int)

Transcriptome analysis revealed the persistent expression of pluripotency genes and underexpression of developmental genes during differentiation in the absence of Kdm5b, suggesting that KDM5B plays a key role in cellular fate changes. (nih.gov)
Promotes self-renewal and maintains pluripotency of human embryonic stem cells and mouse Epi-stem cells when used in combination with CHIR99021 (Kim et al. (stemcell.com)
Functional validation analysis of LCK in self-renewal of hESCs by using LCK inhibitor or gene silencing with siLCK resulted in a loss of undifferentiation characteristics- morphological change, reduced alkaline phosphatase activity and pluripotency gene expression, demonstrating a potential functional role of LCK in self-renewal of hESCs. (ca.gov)
FUNGENES identified the gene subsets that are active in self-renewing cells, during the exit from pluripotency and at specific stages of mouse ES cell differentiation. (europa.eu)
It confers pluripotency on embryonic stem cells and prevents their differentiation towards extraembryonic ENDODERM and trophectoderm (TROPHOBLAST) CELL LINEAGES. (bvsalud.org)

Here, we identify molecular and cellular mechanisms by which Wolbachia influences the molecularly distinct processes of germline stem cell (GSC) self-renewal and differentiation. (geneconvenevi.org)

Induces the differentiation of human PSC-derived alveolar epithelial type II (AETII) to AETI cells (Ghaedi et al. (stemcell.com)
TWS 119 induces neuronal differentiation in pluripotent murine embryonal carcinoma cells and embryonic stem cells at 400 nM. (tocris.com)

At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)

Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
However, MSCs have gained considerable attention for tissue regeneration owing to their differentiation ability with immunomodulatory effects. (techscience.com)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)

In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)

May 25, 2023 The many types of cells in the human body are produced through the process of differentiation, in which stem cells are converted to more specialized types. (sciencedaily.com)
The use of ASCs in regenerative medicine and tissue engineering research has important advantages in comparison with ESCs, since there are no ethical complications and the process of differentiation of these cells is better controlled. (bvsalud.org)

We examined if there are a set of key genes consistently altered during differentiation of hESCs regardless of differentiation conditions. (ca.gov)
To identify a set of key genes consistently altered during differentiation of hESCs regardless of differentiation conditions we have performed microarray analysis on undifferentiated hESCs (H1 and H9) and differentiated EB's and validated our results using publicly available expression array data sets. (ca.gov)
Genes expressed in LRECe revealed retention of some stem-like properties along with up-regulation of differentiation factors. (frontiersin.org)

The telomerase-expressing somatic cells observed in this study are not thought to be immortal, and expression was not correlated with cell cycle distribution or differentiation state. (nih.gov)
Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. (tocris.com)
Our Stem Cells review gives an overview of the use of small molecules in the control of stem cell growth & differentiation and somatic cell reprogramming. (tocris.com)
Understanding the genetic pathways underlying differentiation of ES cells to somatic cells would contribute to future therapeutic strategies for degenerative diseases such as heart disease, diabetes and Parkinson's. (europa.eu)

erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)

Specific properties, such as proliferative and differentiation potential, are slightly different among DSCs. (bvsalud.org)
SCs are not only capable of maintaining themselves (self-renewal) but also give rise to a proliferative 'transit amplifying' compartment and ultimately to postmitotic differentiated cells with specialized functions. (medscape.com)

Undifferentiated I3 cells grew significantly slower but their differentiation potential was greater than I6 and BG01V. (biomedcentral.com)
Depletion of Kdm5b leads to an extension in the self-renewal of ES cells in the absence of LIF. (nih.gov)
We examined hematopoietic stem cells at four stages of differentiation, lineage-committed progenitors, and mature myeloid and lymphoid cells. (nih.gov)
The frequency of telomerase-expressing cells within each population was proportional to the frequency of cells thought to have self-renewal potential. (nih.gov)
To qualify as stem cells, self-renewal has to be shown as well [ 10 ]. (hindawi.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
Discovery of consensus gene signature and intermodular connectivity defining self-renewal of human embryonic stem cells. (ca.gov)
Our understanding of self-renewal and differentiation capacity of human embryonic stem cells (hESCs) remains elusive on the detailed molecular mechanisms. (ca.gov)
In one of the locations-called the bulge- these McSCs undergo self-renewal to maintain a population of immature stem cells. (medicalnewstoday.com)
This differentiation of stem cells to a cell type that performs a specific function is thought to be irreversible. (medicalnewstoday.com)
The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. (nih.gov)
In addition, Wnt signaling also regulates the self-renewal and differentiation of adult stem cells. (novapublishers.com)
This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. (biolegend.com)
In adult tissues, this pathway plays a role in the maintenance and renewal of stem cells, which are cells that help repair tissue damage and can give rise to other types of cells. (medlineplus.gov)
Stem cells (SCs) are undifferentiated cells with self-renewal ability and capacity to differentiate into specialized cell types. (bvsalud.org)
8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)
Finally, there is a discussion about the mechanisms behind the self-renewal and differentiation of stem cells and the role of stem cells in renewal of the body's tissues. (lu.se)
The spleen plays an important role in granulocyte homeostasis also by influencing the elimination of senescent cells and regulatory effects on granulocyte renewal in the bone marrow. (medscape.com)
[ 2 , 3 ] Another study indicates that the T lymphocyte subset in congenital aplasia may be associated with presence of CD4(+) T cells that express the "naïve" phenotype, possible failure in CD8(+) cytotoxic effectors differentiation and tendency to the proinflammatory status of cells, low interleukin (IL) 10 expression, and suboptimal lymphocyte responses to mitogenic stimulation. (medscape.com)
By contrast, transit amplifying cells and differentiated cells are incapable of self-renewal and tissue regeneration. (medscape.com)

The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)

All cell lines differentiated into three embryonic germ lineages in embryoid bodies and into neural cell lineages when cultured in neural differentiation medium. (biomedcentral.com)

Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

Bioinformatics approach for the identification of new molecular markers defining undifferentiated hESCs, interacting partners and interconnectivity analyses may contribute to delineating molecular mechanisms of stem cell self-renewal/differentiation and can be a useful tool to identify molecular factors inducing stemness from different cell types. (ca.gov)

We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)

Here, we show that KDM5B, which demethylates H3K4, is important for ES cell differentiation and presents a barrier to the reprogramming process. (nih.gov)
These findings provide functional insight into the role for KDM5B in regulating ES cell differentiation and as a barrier to the reprogramming process. (nih.gov)
This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. (tocris.com)

In particular, they recovered their capacity for self-renewal and their former differentiation potential, and do not preserve any traces of previous aging. (eurekalert.org)
Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)

By comprehensive genome-wide consensus microarray analyses, we have profiled gene expression signatures that are most significantly affected by differentiation in hESCs from our own microarray data sets as well as publically available microarrays. (ca.gov)
We constructed consensus modules by Weighted Gene Correlation Analysis (WGCNA) and discovered novel markers that are consistently present in undifferentiated hESCs under various differentiation conditions. (ca.gov)

Such CD133+ TICs have also been isolated from three other HCC animal models and patient HCC tissues and were characterized by their NANOG-dependent self-renewal and tumor-initiating activities 6 , 7 , 8 . (nature.com)
ASCs are present in a number of posnatal tissues and are responsible for normal tissue renewal as well as for regeneration and healing after injuries. (bvsalud.org)
Tumors, like the tissues from which they originate, contain a unique SC population that is capable of self-renewal and of sustaining tumor growth indefinitely. (medscape.com)

Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)

This study demonstrates that the developmental characteristic most consistently associated with telomerase expression is self-renewal potential. (nih.gov)
Although HSCs are associated with a fairly clear functional definition regarding their developmental and self-renewal potential, all HSCs do not behave the same. (lu.se)

Furthermore, pathways controlling the self-renewal capacity of normal tissue SCs are frequently deregulated in human cancer. (medscape.com)

The Ras-ERK pathway is involved in Sur8-mediated regulations of differentiation, as the treatment of ERK kinase (MEK) inhibitors blocks the effects of Sur8. (elsevierpure.com)

DNA Methyltransferase 1 Controls Nephron Progenitor Cell Renewal and Differentiation. (bvsalud.org)

Consequently, investigation of molecular heterogeneity in the differentiation potential of HSPC is vital to identify novel regulators that affect generation of specific cell types, especially RBC. (lu.se)

In the present study, a side-by-side comparison of the ability to maintain an undifferentiated state and to self-renew under standard conditions, the ability to spontaneously differentiate into cell types of three germ layers in embryonic bodies, and directed differentiation under neural differentiation-promoting conditions was made between three NIH registered hESC lines I3, I6 and BG01V. (biomedcentral.com)

Signalling pathways influencing stem cell self-renewal and differentiation. (sciencecentral.in)
We are interested in understanding the role of the extracellular matrix in regulating stem cell self-renewal and differentiation, and how this regulation goes awry in cancer. (stanford.edu)
The FUNGENES project aimed to achieve a detailed basic understanding of stem cell self-renewal and differentiation. (europa.eu)

A broader interest of our laboratory is to understand the molecular regulation of HSCs both from the perspectives of self-renewal and differentiation, and how these features become altered upon disease-causing or disease-associated perturbations. (lu.se)

The role and effect of 5hmC on 5mC turnover and hence DNA demethylation, on gene expression per se and stem cell fate and differentiation is a topic of vigorous interest. (stanford.edu)

Our finding has unveiled the novel molecular markers that determine self-renewal and form intramodular hubs. (ca.gov)
Most of analysis has been done under a specific differentiation condition that may present significantly different molecular changes over others. (ca.gov)

All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)

Anatomy67

Organisms12

Diseases9

Chemicals and Drugs71

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Psychiatry and Psychology10

Phenomena and Processes55

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena2

Humanities1

Information Science3

Health Care1

Proliferation17

Progenitor cells3

Pluripotent stem2

Terminal differentiation3

Pluripotency5

Germline stem cell1

Induces2

Osteogenic1

MSCs3

Pathways during differen1

Process of differentiation2

Genes3

Somatic4

Vitro1

Proliferative2

Cells22

Epigenetic1

Lineages1

Hematopoiesis1

Mechanisms1

Epidermal1

Reprogramming3

Capacity for self-renewal2

HESCs2

Tissues3

Proteins1

Developmental2

Human1

Inhibitors1

Methyltransferase1

Potential1

Germ layers1

Cell self-renewal3

Regulation1

Gene expression1

Molecular2

Biological processes1