Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity. (sinica.edu.tw)
Tumour5
BACKGROUND: This study aimed to investigate the expression and clinical significance of Dendritic cell-associated C-type lectin-1 (Dectin-1) in gastric cancer (GC), and to explore the mechanism of Dectin-1 regulating tumour-associated macrophage (TAM)-mediated immune evasion in GC. (bvsalud.org)
Furthermore, blockade of Dectin-1 could reprogramme Dectin-1+ TAMs and reactivate anti-tumour effects of T cells, as well as enhanced PD-1 inhibitor-mediated cytotoxicity of CD8+ T cells against tumour cells. (bvsalud.org)
CONCLUSIONS: Dectin-1 could affect T-cell anti-tumour immune response by regulating the immunosuppressive function of TAMs, leading to poor prognosis and immune evasion in GC patients. (bvsalud.org)
Tumour Growth and Metastasis Analysis The animal study was approved and Glutaminase-IN-1 monitored by the Ethics Committee of Harbin Medical University Cancer Hospital (the ID of animal experiment ethical approval: SYDW2021-056). (bms-911543.com)
For tumour estimation, nude mice were inoculated subcutaneously with 1 107 ZR75-1 cells with different constructs on the right side. (bms-911543.com)
Search1
Results 1-177 of 177 (Search time: 0.003 seconds). (sinica.edu.tw)
Cells9
CSLEX-1, HECA-452, and other widely used mAbs to sLe x and Le x did not bind to WEHI-3 cells and bound at very low levels to murine neutrophils. (ashpublications.org)
Consistent with very low endogenous fucosylation, forced fucosylation of intact WEHI-3 cells or murine neutrophils by exogenous α1-3-fucosyltransferase FTVI and GDP-fucose created many new epitopes for anti-sLe x mAbs such as HECA-452 and CSLEX-1. (ashpublications.org)
1 , 2 P- and E-selectin, expressed on activated platelets and/or endothelial cells, bind to ligands on leukocytes. (ashpublications.org)
BALB/c mice were injected with 1 106 MDA-MB-231 cells labelled with luciferase carrying the indicated constructs into the lateral tail vein. (bms-911543.com)
A) Luciferase reporter genes were determined in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with Glutaminase-IN-1 different concentrations of reporter and myc-ZKSCAN5. (bms-911543.com)
B) Luciferase reporter gene detection in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with expression in ZR75-1 and MDA-MB-231 cells, which were transfected with ZKSCAN5 shRNA, control shRNA, or ZKSCAN5 shRNA plus shRNA-resistant ZKSCAN5 (ZKSCAN5-R). The representative Western blot further showed the expression of ZKSCAN5. (bms-911543.com)
A, B) Cell proliferation and colony formation assays in HLECs cultured in conditioned medium come from ZR75-1 or MDA-MB-231 cells stably infected with lentivirus carrying ZKSCAN5 shRNA or ZKSCAN5 shRNA plus ZKSCAN5-R. The representative Western blot displays the expression of ZKSCAN5. (bms-911543.com)
p 0.01 versus the control shRNA group (A, B). (C, D) Wound healing assays for HLECs cultured in conditioned medium from ZR75-1 or MDA-MB-231 cells, which were Glutaminase-IN-1 stably infected as in (A). The image shown is one of the representative results (C, D). Scale bar: 100 m. (bms-911543.com)
VEGF-D (E, F) Tube formation assays for HLECs cultured in the conditioned medium from ZR75-1 or MDA-MB-231 cells, which were stably infected as in (A). All values shown are the mean SD of triplicate measurements and were repeated three times with analogous. (bms-911543.com)
Medical1
Informed consent was obtained from the patients, and all study protocols were approved by the Institutional Review or Glutaminase-IN-1 Committees of Harbin Medical University Cancer Hospital. (bms-911543.com)
Glycosylation patterns2
Mucin expression and glycosylation patterns on cancer cells differ markedly from healthy cells. (bvsalud.org)
Glycopeptides are enriched without degradation to preserve the glycosylation patterns and structures. (ludger.com)