Seeding human umbilical vein endothelial cells. Medical search. Frequent questions

We hypothesize that blood-derived endothelial progenitor cells (EPCs) have the required proliferative and vasculogenic activity to create vascular networks in vivo. (ashpublications.org)
MATERIALS AND METHODS: Human umbilical vein endothelial cells or human endothelial progenitor cells were treated with bemiparin, fondaparinux or unfractionated heparin, at concentrations reflecting the doses used in clinical practice. (unipi.it)
Furthermore, both bemiparin and fondaparinux caused a significant reduction in an in vitro vasculogenesis-like model, as demonstrated by the decrease of tubule network after co-seeding of endothelial progenitor cells and human umbilical vein endothelial cells. (unipi.it)
Another approach, in situ endothelialization, is based on colonization with ECs in the patient's body, which is possible through transanastomotic growth, transmural infiltration, and endothelialization with endothelial progenitor cells circulating in bloodstream [ 7 ]. (biomedcentral.com)
Previously we found that estrogen aided in this neovasculogenesis via recruitment of bone marrow derived endothelial progenitor cells (BM-EPCs), leading to increased vessel formation and vascular endothelial growth factor (VEGF) production in vivo . (biomedcentral.com)

VEGF is an important component in angiogenesis that binds to heparin, and RGD promotes cell adhesion. (uvm.edu)
Promotion of angiogenesis was evaluated by directly seeding human umbilical vein endothelial cells (HUVECs) onto visible light-crosslinked alginate-based hydrogels. (uvm.edu)
Their in vitro effects on hypoxic cells concerning cell viability, apoptosis, migration, and angiogenesis were detected through gain- and loss-of-function assays. (hindawi.com)
LCN2 was increased by SOX9 and SIRT1 deacetylated SOX9 to promote its nuclear translocation, which further inhibited the viability of human retinal pigment epithelial cells and promoted cell apoptosis and angiogenesis as well as CNV-induced AMD formation. (hindawi.com)
Extract of Artemisia lavandulaefolia Inhibits In Vitro Angiogenesis in Human Umbilical Vein Endothelial Cells. (docksci.com)
In addition, angiogenesis involve complex and diverse cellular actions such as degradation of extracellular matrix, proliferation and migration of endothelial cells, and morphological differentiation of 3,4 endothelial cells to form tubes. (docksci.com)
AQP1 knockout mouse models of human cancers showed marked inhibition of tumor-induced angiogenesis, and in pre-clinical studies of colon adenocarcinomas, forced over-expression of AQP1 was shown to increase angiogenesis, invasion and metastasis. (biomedcentral.com)
The effect of AqB013 on angiogenesis was tested using an endothelial cell tube-formation assay. (biomedcentral.com)
RESULTS: Bemiparin gave a significant decrease of in vitro angiogenesis as shown by the reduction of endothelial cell tubule network, while both fondaparinux and unfractionated heparin did not show any significant effect. (unipi.it)
Tetraspanin Compact disc151 is expressed in endothelial cells and regulates pathologic angiogenesis highly. (biospraysehatalami.com)
Furthermore, key markers in angiogenesis, such as angiogenin and vascular endothelial growth factor (VEGF) were upregulated or secreted in enhanced quantities due to the released by-products of CPO decomposition. (uconn.edu)
Here, it was demonstrated that NV-EXO (normal piglet's Umbilical Veins derived exosomes) promoted angiogenesis within the subdued pro-angiogenesis context of IV-EXO (IUGR piglet's Umbilical Veins derived exosomes). (ijbs.com)

However, the effects of NAC on LPS‑induced apoptosis of human umbilical vein endothelial cells (HUVECs) and the associated mechanisms are not well characterized. (spandidos-publications.com)
The results of CCK‑8 and flow cytometric analyses showed that pretreatment of HUVECs with NAC or p38MAPK inhibitor (SB203580) attenuated LPS‑induced decrease in cell viability and increase in cell apoptosis. (spandidos-publications.com)
they not only effectively induced macrophage polarization to M2 phenotype but also promoted the migration, tube formation, and angiogenic differentiation of human umbilical vein endothelial cells (HUVECs) and facilitated the migration, osteo-differentiation, and extracellular matrix (ECM) mineralization of MC3T3-E1 cells. (biomedcentral.com)
Application of unidirectional pulsatile shear stress to human umbilical vein endothelial cells (HUVECs) decreased PDCD4 protein but not mRNA level. (plos.org)
Therefore, we examined the effect of extract of A. lavandulaefolia on the vascular network formation of human umbilical vein endothelial cells (HUVECs). (docksci.com)
Therefore, we examined the anti-angiogenic effects by A. lavandulaefolia in human umbilical vein endothelial cells (HUVECs). (docksci.com)
Therefore, it is highly probable that tHGA may have anti-inflammatory effects on LPS-induced inflammation, Human Umbilical Vein Endothelial Cells (HUVECs) were used as an in vitro representative of endothelial cells. (upm.edu.my)
HUVECs were seeded to confluence in cell culture insert, where the cells were pretreated with tHGA and induced with LPS. (upm.edu.my)
Quantitative analyses showed the microvessel density was significantly superior to that generated by human dermal microvascular endothelial cells (HDMECs) but similar to that generated by human umbilical vein endothelial cells (HUVECs). (ashpublications.org)
RNAi A retrovirus-delivered shRNA system explained previously4 23 was used to knock down expression in human dermal microvascular endothelial cells (HMECs) and human umbilical vein endothelial cells (HUVECs). (biospraysehatalami.com)
After 15-oxo-ETE treatment, Human umbilical vein endothelial cells (HUVECs) showed more attractive to monocytes, whereas monocyte adhesion is suppressed when treated with PKC inhibitor. (biomedcentral.com)
To this aim, poly(lactide-co-glycolide)/calcium peroxide (PLAGA/CPO) microsphere scaffolds have been fabricated, characterized, and shown to have potential in directing human adipose derived stem cells (hADSCs) and human umbilical vein endothelial cells (HUVECs) towards bone and blood vessel formation in vitro due to the observed release of calcium ions, hydrogen peroxide molecules, oxygen molecules, and hydroxyl ions. (uconn.edu)
In culture with HUVECs, CPO scaffolds were effective in promoting cell adhesion, proliferation, and the formation of microcapillary-like structured as early as 3 days, as determined by scanning electron microscopy (SEM). (uconn.edu)
Up-regulation of miR-150 was able to increase proliferation, migration and tube formation of Human umbilical vein endothelial cells (HUVECs), suggesting a pro-angiogenic role. (ijbs.com)
Expression of chemoresistance molecules was evaluated using flow cytometry in U87MG and human umbilical vein endothelial cells (HUVECs). (biomedcentral.com)

However, few studies have investigated LPS-induced apoptosis of vascular endothelial cells. (spandidos-publications.com)
Furthermore, periodontitis has been shown to be associated with systemic cardiovascular disease ( 5 - 7 ), which suggests its close association with vascular endothelial cells. (spandidos-publications.com)
The aim of this study was to determine the feasibility of heparin and arginyl-glycyl-aspartic acid (RGD) conjugated alginate hydrogels encapsulated with vascular endothelial growth factor (VEGF) as a wound dressing. (uvm.edu)
Vascular endothelial cells, as a monolayer in direct contact with the flowing blood, bear the most of the wall shear stresses and have important homeostatic functions in response to stress [16] . (plos.org)
ECs were treated with oxLDL under HG conditions in the presence or absence of ROS scavengers mitoTEMPO and NAC, p38 inhibitor SB203580, FOXO1 inhibitor AS1842856 or transfected with the TXNIP siRNA, before protein expression levels of intercellular adhesion molecule 1 (ICAM‑1), vascular cell adhesion molecule‑1 (VCAM‑1), phosphorylated vascular endothelial‑cadherin (VE‑cadhedrin), VE‑cadherin and zonula occludens‑1 (ZO‑1) were measured by western blotting. (spandidos-publications.com)
A number of studies have previously suggested that vascular endothelial dysfunction caused by increased junctional permeability is a key event in the development and progression of atherosclerosis and atherosclerotic cardiovascular diseases ( 3 , 4 ). (spandidos-publications.com)
Rat vascular endothelial cells (RVECs) were patterned on collagen-coated nitrocellulose paper to create a tissue chip. (biomedcentral.com)
However, some tissues are exposed to both types of mechanical stimuli, e.g. vascular endothelial cells experience both shear flow and compression [ 16 ]. (biomedcentral.com)

During an inflammatory response, neutrophils move from the blood to the site of inflammation by transmigrating across the endothelial barrier and through the basement membrane (BM) of the surrounding extracellular matrix (ECM). (nature.com)
Additionally, some cytokines found to play an important role in human inflammation such as IL-8 7 , are not expressed by mice. (nature.com)
Toll-like receptors (TLRs) enable innate immune cells, such as macrophages, to recognize a wide variety of microbial ligands, thereby promoting inflammation. (frontiersin.org)
Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)
Endothelial inflammation increases the adhesiveness of monocytes to the endothelium in addition to increasing vascular permeability, promoting diabetic atherogenesis. (spandidos-publications.com)
after transplantation to the recipient region, single cells are vulnerable to the interference of various factors, such as ischemia and inflammation, leading to cell loss or death [ 16 ]. (biomedcentral.com)

We demonstrated leukosomes biocompatibility, endothelium adhesion capability, and tumor target in three-dimensional (3D) settings using CRC cell lines. (frontiersin.org)
The integrity of this monolayer can be disrupted by lipopolysaccharide (LPS), where LPS could increase the permeability of the endothelium via dissociation of cell-to-cell junctions and rearrangement of cytoskeleton. (upm.edu.my)
In the first phase of the extravasation process, selectins such as L-selectin on leukocytes and E- and P-selectin on endothelial cells lead to a loose connection that permits tethering and rolling of leukocytes on the endothelium under hydrodynamic shear [ 10 ]. (biomedcentral.com)
The basis of these experiments is often the use of static parasite binding assays, either using purified receptors or cells, either expressing specific receptors or host-derived endothelium. (biomedcentral.com)

At three weeks, the influx of endothelial cells and the synthesis of immature extracellular matrix components by fibroblasts began. (encyclopedia.pub)
The main advantage of hydrogels for microfluidic cell cultures, however, is their mimicry of extracellular matrix structures including adequate porosity for cellular organization, biocompatibility, and representative stiffness, all key parameters that promote native-like tissue function. (heraldopenaccess.us)
Despite recent achievements of microfluidic 2D cell culture systems [9], they still do not address the fact that in vivo cells coexist in 3D communities that are influenced by spatial orientation of cells and cell-to-cell contact within the extracellular matrix [10]. (heraldopenaccess.us)
Exosomes are membranous vesicles (30-100 nm in diameter) that are released by a variety of cell types into the extracellular space. (ijbs.com)
Our results proposed 16-h perfusion of 0.25% sodium dodecyl sulfate (SDS) + 0.5% Triton X-100 combination to the vessel as an optimal decellularization protocol in terms of cell elimination as well as extracellular matrix preservation. (biomedcentral.com)

Apoptosis refers to the self-regulated and orderly death of cells controlled by genes in order to maintain the stability of internal environment ( 8 ). (spandidos-publications.com)
The apoptosis of endothelial cells is a key event that may impair the integrity of the vessel wall and lead to the formation of atherosclerotic plaques ( 9 ). (spandidos-publications.com)
The death receptor pathway plays an important role in cell apoptosis ( 12 ). (spandidos-publications.com)
A series of caspases proteins have been shown to be activated by the death induced signal complex [a form of death ligand that binds to the corresponding death receptor on the cell surface ( 13 )], which in turn induces apoptosis. (spandidos-publications.com)
Low PDCD4 level was associated with reduced proliferation but not apoptosis or phosphorylation of endothelial nitric oxide synthase caused by pulsatile shear stress to help maintain the homeostasis of endothelial cells. (plos.org)
Decreased stability of CD151-deficient endothelial networks may result from increased apoptosis reduced cell proliferation rates and/or altered cell movement. (biospraysehatalami.com)
Mechanism of pingyangmycin-induced apoptosis of cultured human umbilical vein endothelial cells. (geneticsmr.com)
In this study, we investigated the effects of pingyangmycin (PYM) on the growth inhibition and apoptosis of human umbilical vein endothelial cells (HUVEC). (geneticsmr.com)
Correlation between TRAIL and caspase-8 expression and their relationship with cell proliferation and apoptosis in human osteosarcoma. (geneticsmr.com)
Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
1 - 4 Multiple mechanisms may be involved in its potent antitumor effect, including induction of apoptosis, cell cycle arrest, telomerase inhibition, antiangiogenesis activity, and an antimetastasis effect. (dovepress.com)
To investigate the putative anti-proliferative effect, apoptosis assay, microRNA expression profile and study of cell cycle were performed. (biomedcentral.com)

Under normal condition, the endothelial cells form a compact monolayer to line the blood vessel. (upm.edu.my)
In addition, intact endothelial monolayer inhibits the proliferation of smooth muscle cells (SMCs), limiting the risk of intimal hyperplasia. (biomedcentral.com)
It has been repeatedly demonstrated that the presence of a 3D matrix promotes many biologically relevant functions otherwise not observed in 2D monolayer cell cultures [11]. (heraldopenaccess.us)
Furthermore, monocyte transmigration through the endothelial monolayer was significantly increased by oxLDL treatment under HG conditions. (spandidos-publications.com)
Subsequently, rosmarinic acid treatment significantly reversed oxLDL‑induced overexpression of adhesion molecules and monocyte‑EC adhesion, oxLDL‑induced endothelial junction hyperpermeability and monocyte transmigration through the endothelial monolayer under HG conditions, in a dose‑dependent manner. (spandidos-publications.com)
Endothelial cells (ECs) form a semi-permeable monolayer that separates the bloodstream from underlying tissues and regulate the infiltration of blood cells and proteins through the vessel wall ( 1 , 2 ). (spandidos-publications.com)

These results suggest that rosmarinic acid can exert a protective effect against oxLDL‑mediated endothelial dysfunction under HG conditions by reducing the interaction between monocytes and ECs in addition to preventing monocyte diapedesis. (spandidos-publications.com)

As a proof of principle, Human Umbilical Vein Endothelial Cells (HUVEC) and Human Mesenchymal Stem Cells (hMSC) were seeded and cultured into the cell culture unit. (unimi.it)

Estrogen treated TG1-1 cells secreted higher levels of VEGF which were comparable to secreted levels from cells grown under hypoxic conditions. (biomedcentral.com)

Similar patterns were observed in normal (NA) and IUGR (IA) umbilical arteries as well. (ijbs.com)
Structurally, the umbilical cord consists of 2 umbilical arteries (UA) and 1 umbilical vein (UV). (ijbs.com)
Pediatric vascular disease consists of conditions that are caused by abnormal development of arteries, veins, or other blood vessels. (medscape.com)

Human umbilical vein endothelial cells formed confluent vessels against collagen I and geltrex mix, a mix of basement membrane extract and collagen I. TNF-α-stimulation of vessels upregulated inflammatory cytokine expression and promoted neutrophil transmigration. (nature.com)
Inhibition of neutrophil proteases inhibited neutrophil transmigration on geltrex mix, but not collagen I. These findings highlight the important role of the ECM in determining cell phenotype and response to inhibitors. (nature.com)

To test this, EPCs isolated from human umbilical cord blood or from adult peripheral blood, and human saphenous vein smooth muscle cells (HSVSMCs) as a source of perivascular cells, were combined in Matrigel and implanted subcutaneously into immunodeficient mice. (ashpublications.org)
Tissue engineering of native vessels is an alternative approach for patients with vascular disease who lack sufficient saphenous vein or other suitable conduits for autologous vascular graft. (biomedcentral.com)

Investigation of the miRNA transcriptome of umbilical cord vein and artery exosomes between IUGR and normal littermates showed significant differences between umbilical veins from normal (NV) and IUGR (IV) piglets. (ijbs.com)

Equally, studying neutrophils using in vivo animal models has important ethical implications and can inaccurately model human physiology 5 . (nature.com)
This lack of translation from current in vitro and in vivo models into humans has led to challenges in drug discovery 8 . (nature.com)
Cell and animal experiments were performed to characterize the immunomodulatory, angiogenic, and osteogenic capabilities of the piezoelectric hydrogel scaffold in vitro and in vivo. (biomedcentral.com)
This maturation correlated with a decrease in the degree of vascularization in vivo, which could be compensated for by increasing the number of EPCs seeded into the implants. (ashpublications.org)
1 One strategy is to seed the appropriate cells on a biodegradable scaffold engineered with the desired mechanical properties, followed by stimulation of cell growth and differentiation in vitro, such that, on implantation in vivo, the engineered construct undergoes remodeling and maturation into functional tissue. (ashpublications.org)
The potential of recombinant ALP to block the interactions of leukocytes with the endothelial lining was concomitantly investigated in vitro and in vivo . (biomedcentral.com)
In vitro permeability assays, trans-endothelial invasion assays, in vivo permeability assays and tissue fluorescence staining were conducted to examine vascular permeability. (thno.org)

These computer chip-sized devices provide cells with a more physiological 3D environment that supports cell differentiation, enabling organ-like functions. (nature.com)
hADSCs seeded on CPO scaffolds adhered, spread, and underwent osteogenic differentiation up to 28 days, as shown by SEM, alkaline phosphatase activity, alizarin red staining, and elevated levels of osteocalcin, a late stage bone matrix protein. (uconn.edu)

The expression of caspase‑3, Bax, Bcl‑2, phosphorylated (p)‑p38MAPK/total (t‑)p38MAPK and p‑endothelial e nitric oxide synthase (eNOS)/t‑eNOS proteins were determined by western blotting. (spandidos-publications.com)
In physiological conditions, the lumen of the blood vessel is covered with endothelial cells (ECs), which actively counteract the processes of platelet aggregation and blood coagulation through the synthesis and secretion of various bioactive substances e.g.: nitric oxide, heparan sulphate, prostacyclin [ 6 ]. (biomedcentral.com)

This multi-step process is enabled by inflammatory receptors and molecules expressed by the endothelial cells which capture the neutrophils and support their extravasation, alongside gradients of chemokines that guide the neutrophils through the ECM. (nature.com)
Therefore, we investigated the in vitro effect of DHEA on the expression pattern of adhesion molecules of human endothelial cells and neutrophils. (biomedcentral.com)
Neutrophils were freshly isolated from blood of 10 male healthy volunteers, stimulated the same way as endothelial cells and analyzed for surface expression of L-selectin, CD11b and CD18. (biomedcentral.com)

Activated by cytokines and chemokines, leukocytes and endothelial cells express distinct adhesion molecules on their cell surfaces [ 9 ]. (biomedcentral.com)
These adhesion molecules enable the deceleration of blood cells on the endothelial layer in order to enable subsequent diapedesis. (biomedcentral.com)
These findings support the notion that the newly uncovered properties of ALP to interfere with cytokine signalling and upregulation of adhesion molecules in endothelial cells are likely to contribute to the therapeutic potential of ALP in immune-complex-induced tissue injury. (biomedcentral.com)
Endothelial dysfunction, as a comprehensive index of the overall CVD risk factor burden includes three main consequences, exposure of adhesion molecules, the activation and aggregation of platelets, cholesterol accumulation [ 20 ]. (biomedcentral.com)

Aquaporins (AQP) are water channel proteins that enable fluid fluxes across cell membranes, important for homeostasis of the tissue environment and for cell migration. (biomedcentral.com)
Our hypothesis is that inhibition of AQP1 will reduce migration and invasiveness of colon cancer cells, and the migration and tube-forming capacity of endothelial cells in vitro . (biomedcentral.com)
The effect of varying concentrations of the AQP1 inhibitor AqB013 was tested on human colon cancer cell lines expressing high versus low levels of AQP1, using wound closure (migration) assays, matrigel invasion assays, and proliferation assays. (biomedcentral.com)
0.0001) treated with 160 or 320 μM AqB013 respectively, whereas there was no effect on migration of HCT-116 cells with low AQP1 expression. (biomedcentral.com)
We assessed the effect of CD151 loss on cell migration and survival and found that CD151 silencing did not significantly alter (1) EC motility in Transwell migration (supplemental Physique 3A) and wound healing (data not shown) assays and (2) EC viability after being plated on Matrigel immediately (supplemental Figure. (biospraysehatalami.com)
Cell migration ability melanoma A375 was monitored by using cell scratch assay. (biomedcentral.com)
Tan II A reduced CXCL12-induced A375 cell invasive ability and migration in a dose dependent manner. (biomedcentral.com)
Soluble factors in conditioned media from E 2 treated breast cancer cells also lead to migration and tube formation of human umbilical vein endothelial cells (HUVEC) in vitro . (biomedcentral.com)
particularly, the effect of miR-195-5p modulation seemed to affect cell cycle, while miR-107 seemed to be implicated in the inhibition of cells migration. (biomedcentral.com)

Cell viability was assessed using the Cell Counting Kit‑8 (CCK‑8) assay. (spandidos-publications.com)
The objective of the current study was to evaluate the influence of flow driven shear stress on the viability of cultured cells inside the luminal wall of a serpentine network. (mdpi.com)
The cell viability of human umbilical vein endothelial cells (HUVEC) in high doses of these two contrast agents were measured using the xCELLigence Real-Time Cell Analysis (RTCA) S16 Instrument. (dovepress.com)
After 24h, cell viability, proliferation, tubule formation and angiogenic molecular mechanisms, such as activation of the serine/threonine kinase AKT, were assessed. (unipi.it)
The application of hydrogels as a matrix for 3-dimensional cell cultures has become an indispensable tool in tissue engineering, biotechnology and biomedical research due to the improved functionality and viability of the in vitro biological system. (heraldopenaccess.us)
We also developed a novel strategy to eliminate the remnant detergents from the scaffold and increase cell viability by incubating acellular scaffolds with Bio-Beads SM-2 resin. (biomedcentral.com)
These findings reveal that the incubation of the scaffold with Bio-Beads SM-2 is a novel and promising approach for increasing cell viability and growth within the scaffold. (biomedcentral.com)
In conclusion, our results provide a platform in which xenograft vessels are decellularized properly in a short time, and the recellularization process is significantly improved after the bioconjugation of the acellular scaffold with apelin-13 in terms of cell adhesion and viability within the scaffold. (biomedcentral.com)

Expression of SIRT1/SOX9/LCN2 in the hypoxic cells was determined, and their interactions were predicted by bioinformatics websites and followed by the verification by luciferase assay and chromatin immunoprecipitation (ChIP). (hindawi.com)
An IL-1β-activated endothelial layer formed by a murine glomerular cell line (glEND.2) was used to assay the interaction with human leukocytes in vitro . (biomedcentral.com)
Cell proliferation was assessed by using methyl thiazol tetrazolium (MTT) assay. (biomedcentral.com)
This In Vitro Vascular Permeability Assay kit employs a 24-well plate and provides an efficient system for evaluating the effects of chemicals & drug compounds on endothelial cell adsorption, transport & permeability. (emdmillipore.com)

Fibrinogen (FG) adsorption on surfaces with controlled fraction of -OH groups was investigated with AFM and correlated to the initial interaction of primary endothelial cells (HUVEC). (ibecbarcelona.eu)
Further, hypoxia and estrogen are interchangeable as both similarly modulate epithelial-endothelial cell interaction. (biomedcentral.com)

Studies have shown that periodontitis is associated with altered expression of inflammatory mediators in gingival fibroblasts and periodontal membrane cells ( 3 , 4 ). (spandidos-publications.com)
Subsequently, the scaffold was bioconjugated with apelin-13 using the EDC-NHS linker and acellular scaffolds were recellularized using fibroblasts, endothelial cells, and smooth muscle cells. (biomedcentral.com)

Programmed cell death 4 (PDCD4) is an important tumor suppressor in the development of various human cancers [1] and inhibits translation rather than transcription. (plos.org)

Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
When the cell's DNA and protein damaged, the cells maintained its cellular homeostasis through autophagy. (biomedcentral.com)

It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
The extract also showed a dose dependent growth inhibition of all tested cell lines, IC 50 values against HCT-116, HUVEC, CCD-18CO and HT-29 were 14, 23, 50 and 52 μg mL -1 , respectively. (scialert.net)

This micrograph shows a prostate cancer cell (orange) suspended between cellulose nanocrystal clusters (green) doped into Poly(ethylene glycol)-diacrylate (PEGDA) hydrogel scaffolds. (msc-smc.org)
In this study, inkjet bioprinting was used to deposit a cell-laden bioink into additively manufactured scaffolds of differing architectures to engineer fibrocartilage grafts with user defined collagen architectures. (regenhu.com)
The architecture of MEW scaffolds can be tailored to not only modulate the directionality of the collagen fibres laid down by cells, but also to tune the anisotropic tensile mechanical properties of the resulting constructs, thereby enabling the engineering of biomimetic meniscal-like tissues. (regenhu.com)
Endothelial coverage after 1, 3, and 7 days of culture on flat samples (2D model) was higher on Nano prostheses as compared with Micro scaffolds. (biomedcentral.com)
This work details the first use of CPO as an inexpensive, stable, chemical agent capable of delivery from biodegradable polymeric microsphere scaffolds to promote the angiogenic behaviors of endothelial cells as well as osteo- and angiogenic behaviors of adult stem cells, and provides a strong foundation for future work exploring the molecular mechanisms regulating the observed behaviors as well as potential success in clinically relevant settings as a bone graft substitute. (uconn.edu)
The immunohistochemical (IHC) staining of CD31, α-SMA, and vimentin markers suggested placement of seeded cells in the suitable sites and considerable elevation of cell attachment within the scaffolds bioconjugated with apelin-13 compared to the non-bioconjugated, and decellularized groups. (biomedcentral.com)

More recent research applications include microchips for genomics, proteomics and cell-based assays. (heraldopenaccess.us)
Investigations into this adhesive phenomenon have used protein and cell-based adhesion assays to quantify the ability of infected red blood cells to bind. (biomedcentral.com)
Cell based assays were also used as inputs to one of the automated systems (ImageSXM) and produced variable, but encouraging, results. (biomedcentral.com)
The use of automated systems for counting cell-based assays was also considered. (biomedcentral.com)

In addition, the presence of EVs reduced inflammatory responses in Pam 3 CSK 4 -treated endothelial cells and HEK Dual reporter cells, demonstrating that TLR2-EVs can act as decoy receptors. (frontiersin.org)

Therefore, the present study aimed to elucidate the mechanisms involved in oxLDL‑induced endothelial permeability and monocyte diapedesis under HG conditions, in addition to the potential effects of rosmarinic acid. (spandidos-publications.com)
Chronic metabolic disorders, such as hyperglycemia and dyslipidemia, promote endothelial dysfunction and eventually leakage, resulting in increased monocyte diapedesis ( 5 - 7 ). (spandidos-publications.com)

Human Umbilical Vein Endothelial Cells were seeded and grown within a microfluidic chip until they were confluent. (msc-smc.org)
This review focuses primarily on recent advances in biologically inspired microfluidic systems that are based on 3-dimensional hydrogel cell cultures and discusses advantages and current challenges, various applications of microfluidic hydrogel cell cultures and future perspectives. (heraldopenaccess.us)
These microfluidic cell cultures are considered potential candidates to provide next generation cell analysis systems. (heraldopenaccess.us)
Starting from single cell analysis using miniaturized flow cytometers [3] a variety of microfluidic devices have been developed for cell studies to investigate cell transport and cultivation in the absence and presence of concentration and temperature gradients or shear force conditions. (heraldopenaccess.us)
The main benefit of microfluidic systems for cell culture analysis is that they can perform a number of crucial liquid handling steps including cell loading, nutrient supply and waste removal under physiologically relevant shear force conditions, all while offering real time microscopy [4]. (heraldopenaccess.us)
In addition, shear flow can easily be applied to microfluidic tissue models, where the microfluidic channels mimic the network of vessels found in human tissues and organs. (biomedcentral.com)
In fact, shear flow and compression stimuli require totally different tissue model platforms: shear flow has typically been demonstrated on silicone-based microfluidic tissue models, while compression on 3D gel-based cell culture models. (biomedcentral.com)
Application of stretch and/or strain to in vitro tissue models also requires 3D gel-based cell culture models that are stretchable and/or compressible, which is inappropriate for silicone-based microfluidic models. (biomedcentral.com)

As far as LEV is concerned, in 2010 Bobustuc hypothesized that AED may modulate O-6 methylguanine-DNA methyltransferase (MGMT), a DNA repair protein that has an important role in tumor cell resistance to alkylating agents, and LEV was reported as the most potent MGMT inhibitor among several AED [ 10 ]. (biomedcentral.com)

Endothelial cells derived from human umbilical cord were subjected to an lipopolysaccharide (LPS) challenge. (biomedcentral.com)

Interestingly, the addition of catalase, an enzyme that catalyzes the decomposition of hydrogen peroxide to oxygen, accelerated bone formation as well as promoted the induction of hADSCs towards the endothelial phenotype, as observed by changes in cellular morphologies as well as the formation of microcapillary-like structures. (uconn.edu)

The effects of oxLDL‑mediated inflammasome activation on endothelial permeability under HG conditions, in addition to the effects of rosmarinic acid on these oxLDL‑mediated processes, also remain poorly understood. (spandidos-publications.com)
In addition, oxLDL‑stimulated ECs under HG conditions exhibited increased phosphorylated VE‑cadherin protein levels and decreased ZO‑1 protein expression levels compared with those in untreated ECs, suggesting increased endothelial permeability. (spandidos-publications.com)

tHGA is able to preserve barrier integrity of LPS-induced endothelial cells via preservation of actin cytoskeleton and junctional proteins. (upm.edu.my)

Dysfunctional umbilical cord blood (UCB) is a key factor for the development of intrauterine growth restriction (IUGR) in utero . (ijbs.com)

Using the murine mammary cell line TG1-1 we observed estrogen (E 2 ) lead to an up regulation of hypoxia inducible factor-1 (HIF-1), an effect abrogated by the anti-estrogen Fulvestrant and the HIF-1 inhibitor YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole) suggesting the interchangeability of hypoxia and estrogen mediated effects. (biomedcentral.com)

It was hypothesized that an elastic and tough alginate-based hydrogel would be amendable to RGD and heparin conjugation to support cell proliferation and in vitro tubular network formation. (uvm.edu)
Crosslinked alginate-heparin maintained cell adhesion and promoted tubular formation. (uvm.edu)

The biological activity of the surface-induced FG network to provide 3D cues in a potential tissue engineered scaffold, making use of electrospun PEA fibers (-OH(0)), seeded with human umbilical vein endothelial cells was investigated. (ibecbarcelona.eu)
6 To overcome the problem of vascularization, strategies such as embedding angiogenic factors into the scaffold to promote ingrowth of microvessels, fabrication technologies to create polymers containing vessellike networks, and prevascularization of matrices prior to cell seeding have been proposed. (ashpublications.org)
Scaffold architecture was found to influence the spatial organization of the collagen network laid down by the jetted cells, with higher aspect ratios (1:4 and 1:16) supporting the formation of structurally anisotropic tissues. (regenhu.com)

We investigated whether atheroprotective unidirectional pulsatile shear stress affects the expression of PDCD4 in endothelial cells. (plos.org)
En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)

Enhanced ZNF281 transcriptionally regulated CCL2 and CCL5 expression to activate P38 MAPK signaling in endothelial cells. (thno.org)

Cells were fixed and stained to identify their nuclei (blue, DAPI), actin (green, phalloidin-AF488), and VE-Cadherin proteins (red, antibody-AF555), and imaged with standard laser-scanning confocal microscopy, Plan apo 10x (Na 0.45, WD 4.0 mm 10X objective lens, Nikon A1). (msc-smc.org)
The host cell exerts its self-clearing of toxic substances such as damaged proteins and organelles through autophagy processes [ 7 ]. (biomedcentral.com)

The present study results indicated that compared with the same number of 2D-cultured cells, microtissue could secrete more nerve regeneration related cytokines to promote SCs proliferation and axons growth. (biomedcentral.com)

Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
Tan IIA promoted autophagic body production and increased autophagy-associated protein beclin-1 and LC3-II expression in A375 cells. (biomedcentral.com)
We concluded that Tan II A reduced A375 cells proliferation by activation of autophagy production, blocked PI3K- Akt - mTOR - p70S6K1 signaling pathway, increased autophagic related gene beclin-1, LC3-II protein expressions and induced autophagocytosis. (biomedcentral.com)
Estrogen modulation of HIF-1 and subsequent effects on endothelial cells is dependent on the Akt/PI3K pathway and protein synthesis as validated by the use of the inhibitors wortmannin and cycloheximide which abrogated estrogen's effects respectively. (biomedcentral.com)

Endothelial network formation on 3D matrices Matrigel was plated in 48-well plates and incubated at 37°C for 1 hour for gelation. (biospraysehatalami.com)

The development of biomimetic nanoparticles (NPs) has revolutionized the concept of nanomedicine by offering a completely new set of biocompatible materials to formulate innovative drug delivery systems capable of imitating the behavior of cells. (frontiersin.org)

In addition to the incorporation of frequently used bioactive macromolecules and nanomolecules, burgeoning research has found that multiple physiological cues, including mechanics, electricity, and magnetism, represent fresh prospects for bone regeneration by influencing bone-related cell behaviors and cell maturation events [ 12 ]. (biomedcentral.com)

2 Examples of this approach include blood vessels and cardiovascular substitutes, where autologous vascular cells have been used for this purpose without immune rejection. (ashpublications.org)
Emerging evidence indicates that the role of Grape Seed Extract (GSE) in support of cardiovascular health may extend beyond its important antioxidant functions. (lifesourcevitamins.com)
The umbilical cord changes, with respect to the physical dimensions and composition, can be considered reflective of changes in the fetal cardiovascular system [ 7 ]. (ijbs.com)

We sought to explore the possible mechanism by which Tan II A regulated cell proliferation through autophagy signaling pathway in A375 cells. (biomedcentral.com)

Transwell chamber experimental was performed to assess the effect of Tan II A on A375 melanoma cell invasion ability. (biomedcentral.com)

The combination of 3-dimensional hydrogel cell cultures with microchip technology further allows (i) spatial and temporal control of cell growth, (ii) application of defined mechanical (e.g. shear, strain, stretch) and chemical (e.g. gradients) stimuli, as well as (iii) monitoring of dynamic cellular responses using integrated sensing strategies. (heraldopenaccess.us)

Thus, we hypothesized that the upregulated membrane-bound TLR2 might serve as a precursor for soluble TLR2 (sTLR2), known to antagonize TLR2-dependent cell actions. (frontiersin.org)
We found that Tan IIA inhibited melanoma A375, MV3, and M14 cell proliferation in dose and time dependent manner. (biomedcentral.com)

However, the action of PDCD4 in endothelial cells is unclear. (plos.org)

For the vascular fraction, the optimized formulation consisted of 5% GelMA, 0.8 mg·ml−1 collagen and 1 mg·ml−1 fibrinogen with a higher crosslinked density, which led to enhanced vascular cell connectivity. (regenhu.com)

Anatomy39

Organisms8

Diseases9

Chemicals and Drugs137

Analytical, Diagnostic and Therapeutic Techniques and Equipment19

Phenomena and Processes42

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science3

Progenitor cells5

Angiogenesis12

HUVECs15

Vascular endothelial8

Inflammation6

Endothelium4

Extracellular5

Apoptosis14

Monolayer6

Mediated endothelial dysfunction1

Mesenchymal Stem1

VEGF1

Arteries3

Transmigration2

Saphenous vein2

Artery1

Vivo7

Differentiation2

Nitric2

Neutrophils3

Adhesion molecules4

Migration9

Viability8

Assay4

Interaction2

Fibroblasts2

Inhibits1

Homeostasis2

Inhibition2

Scaffolds6

Assays4

Inflammatory1

Monocyte2

Microfluidic8

Tumor cell1

Lipopolysaccharide1

Phenotype1

Permeability2

Junctional1

Cord blood1

Inhibitor1

Heparin2

Scaffold3

Shear stress2

MAPK1

Proteins2

Cytokines1

Protein4

Matrices1

Behavior1

Bioactive1

Cardiovascular3

Pathway1

Invasion1

Spatial1

Dependent2

Unclear1

Collagen1