Secretome induces cell senescence. Medical search. Frequent questions

Senescent cells are characterized by an upregulation of proinflammatory cytokines, which is termed the senescence-associated secretory phenotype (SASP). (nature.com)
SnCs exhibit irreversible growth arrest accompanied by increased expression of cyclin-dependent kinase inhibitors (CDKi) such as p16 INK4a , and p21 Cip1 , accumulation of DNA damages, and secretion of diverse bioactive molecules known as the senescence-associated secretory phenotype (SASP). (aging-us.com)
Senescence-associated ß-galactosidase activity (SA-ß-gal), Î³-H2A.X, p53, p21, and senescence-associated secretory phenotype (SASP) were evaluated. (bvsalud.org)
Senescence-associated secretory phenotype (SASP) is responsible for chemotherapy adverse effects: drug resistance and the induction of cancer cell proliferation, migration, and invasion. (phiab.com)
This is called the senescence-associated secretory phenotype - or secretome. (institutducerveau-icm.org)
Senescent cells are characterized by proliferation arrest and a specific secretome, senescence-associated secretory phenotype (SASP). (ubatubasat.com)
On the other hand, senescent cells secrete many proinflammatory cytokines, chemokines, growth factors, and proteases, collectively termed as the senescence-associated secretory phenotype (SASP), which causes chronic inflammation and tissue dysfunction. (molcells.org)

Senescent cells contribute to the aging phenotype, including frailty syndrome, sarcopenia, and aging-associated diseases. (wikipedia.org)
Although senescent cells can no longer replicate, they remain metabolically active and commonly adopt an immunogenic phenotype consisting of a pro-inflammatory secretome, the up-regulation of immune ligands, a pro-survival response, promiscuous gene expression (pGE), and stain positive for senescence-associated β-galactosidase activity. (wikipedia.org)
Senescent cells can undergo conversion to an immunogenic phenotype that enables them to be eliminated by the immune system. (wikipedia.org)
This phenotype consists of a pro-inflammatory secretome, the up-regulation of immune ligands, a pro-survival response, promiscuous gene expression (pGE) and stain positive for senescence-associated β-galactosidase activity. (wikipedia.org)
As viral replication is usually more efficient in senescent cells, many viruses, including CMV and probably SARS-CoV-2, promote this phenotype in host cells to facilitate invasion (19, 21, 22). (ubatubasat.com)
This ability of MSCs to adopt a different phenotype in response to sensing an inflammatory environment is not captured in assays that are commonly used to characterize these cells, but it is crucial for understanding their therapeutic potential in immune-mediated disorders. (pdffox.com)

Senescent cells display persistent DDR that appears to be resistant to endogenous DNA repair activities. (wikipedia.org)
Such senescent cells in mammalian culture and tissues retain DSBs and DDR markers. (wikipedia.org)
The nucleus of senescent cells is characterized by senescence-associated heterochromatin foci (SAHF) and DNA segments with chromatin alterations reinforcing senescence (DNA-SCARS). (wikipedia.org)
Senescent cells affect tumour suppression, wound healing and possibly embryonic/placental development and a pathological role in age-related diseases. (wikipedia.org)
December 2, 2022 -- Scientists at the Salk Institute for Biological Studies have found that neurons from people with Alzheimer's disease show deterioration and undergo a late-life stress process called cellular senescence, while senescent cells could be a way to slow neuroinflammation and neurodegeneration. (scienceboard.net)
With studies linking the persistence of senescent cells late in life to tissue dysfunction and age-related disease, scientists at the Salk Institute questioned if the phenomenon plays a role in Alzheimer's, most cases of which currently have no known origin. (scienceboard.net)
An in vitro test found the combination of dasatinib and quercetin, molecules that remove senescent cells in conditions such as osteoarthritis, returned the number of senescent neurons to normal levels. (scienceboard.net)
University of California, San Francisco researchers have found some senescent cells help to heal damaged tissues, raising questions about the merits of. (scienceboard.net)
Senescent cells (SnCs) have been described to accumulate in osteoarthritis (OA) joint tissues in response to injury, thereby participating in OA development and progression. (aging-us.com)
Indeed, senescent cells (SnCs) are described to accumulate in joint tissues in response to injury and during aging, thereby participating in its development and progression [ 8 - 10 ]. (aging-us.com)
Different pharmacological strategies have been used to eliminate senescent cells by inducing their apoptosis or modifying their secretome. (bvsalud.org)
published in Nature Communications , Isabelle Le Roux (CNRS) and her colleagues from the "Genetics and development of brain tumors" team at Paris Brain Institute have shown that the elimination of senescent cells, i.e., cells that have stopped dividing, can modify the tumor ecosystem and slow its progression. (institutducerveau-icm.org)
They found, in varying proportions (0.4% to 7% of the original mass of glioblastoma), senescent cells of different cell types - tumoral, immune, or glial - located mainly in areas of malignant cell proliferation, as well as in necrosis zones. (institutducerveau-icm.org)
Eventually, we could consider treating patients with senolytics, i.e., molecules that target senescent cells to destroy them, says the researcher. (institutducerveau-icm.org)
however, senescence in living organisms is poorly understood, largely because of technical limitations relating to the identification and characterization of senescent cells in tissues and organs. (elsevierpure.com)
Furthermore, newly recognized beneficial signaling functions of senescence suggest that indiscriminately targeting senescent cells or modulating their secretome for anti-aging therapy may have negative consequences. (elsevierpure.com)
Here we discuss current progress and challenges in understanding the stressors that induce senescence in vivo, the cell types that are prone to senesce, and the autocrine and paracrine properties of senescent cells in the contexts of aging and age-related diseases as well as disease therapy. (elsevierpure.com)
However, damage accumulated during ageing increases the number of senescent cells and this contributes to the chronic inflammation and deregulation of the immune function, which increases susceptibility to infectious disease in ageing organisms. (whiterose.ac.uk)
Because age-related cellular senescence and type 2 diabetes (T2D) have been recognised as risk factors for CRC development, the recent finding that type 2 diabetic patients present an elevated circulating volume of senescent cells raises the question whether type 2 diabetes facilitates the process of CRC tumorigenesis by inducing premature cell senescence. (frontiersin.org)
Over lifetime, due to the action of several stressors such as DNA damage and telomere shortening, senescent cells accumulate in the organism and release a variety of pro-inflammatory cytokines responsible for low-grade inflammation. (frontiersin.org)
Much effort has been recently made to therapeutically target detrimental effects of cellular senescence including selectively eliminating senescent cells (senolytics) and modulating a proinflammatory senescent secretome (senostatics). (molcells.org)
This led to an intriguing hypothesis that senescence contributes to aging, and thus the elimination of senescent cells might delay the aging process. (molcells.org)
Upon the administration of a synthetic drug called rapalog, p16 INK4A positive senescent cells are removed in this system by apoptosis. (molcells.org)
The effects of the elimination of senescent cells were simply striking. (molcells.org)
A fluorogenic substrate is added directly to senescent cells in a 35 mm dish. (cellbiolabs.com)

Cellular senescence is a stable growth arrest that is implicated in tissue ageing and cancer. (nature.com)
Cellular senescence is a phenomenon characterized by the cessation of cell division. (wikipedia.org)
Hayflick's discovery of mortal cells paved the path for the discovery and understanding of cellular aging molecular pathways. (wikipedia.org)
Cellular senescence can be initiated by a wide variety of stress inducing factors. (wikipedia.org)
Depletion of NAD+ can lead to DNA damage and cellular senescence in vascular smooth muscle cells. (wikipedia.org)
Two proteins, senescence-associated beta-galactosidase and p16Ink4A, are regarded as biomarkers of cellular senescence. (wikipedia.org)
Recently, the role of telomeres in cellular senescence has aroused general interest, especially with a view to the possible genetically adverse effects of cloning. (wikipedia.org)
BRAFV600E and Ras are two oncogenes implicated in cellular senescence. (wikipedia.org)
Based on the findings, Gage and his collaborators speculated that senotherapeutics, drugs that target cellular senescence, may prevent or treat Alzheimer's. (scienceboard.net)
Several hallmarks of cellular senescence, such as cell cycle arrest, expression of cyclin-dependent kinase inhibitors, DNA damages, and senescence-associated secretory profile were evaluated. (aging-us.com)
Etoposide-induced senescence model may help investigate the initiation of cellular senescence in chondrocytes, and provide a useful model to develop therapeutic approaches to target senescence in OA. (aging-us.com)
Cellular senescence has been described as one of the major drivers of aging [ 6 ] and has been implicated in the pathogenesis of many age-related diseases [ 7 ]. (aging-us.com)
Recently, cellular senescence has emerged as a new target to treat OA [ 8 ]. (aging-us.com)
Because a specific marker for cellular senescence has yet to be identified, a combination of markers should be used to identify SnCs [ 14 ]. (aging-us.com)
Considering the role of cellular senescence in age-related diseases including OA, the therapeutic potential of senolytic (drugs that induced SnCs death) and senomorphic (drugs that modulate the SASP) compounds have been contemplated with growing interest [ 15 ]. (aging-us.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
One recent approach consists in targeting a key biological process: cellular senescence. (institutducerveau-icm.org)
The secretome can influence the cellular environment in a beneficial or detrimental way. (institutducerveau-icm.org)
Modulating cellular senescence could therefore constitute a new therapeutic avenue to be combined with conventional treatments - to increase their effectiveness. (institutducerveau-icm.org)
Cellular senescence, a process that imposes permanent proliferative arrest on cells in response to various stressors, has emerged as a potentially important contributor to aging and age-related disease, and it is an attractive target for therapeutic exploitation. (elsevierpure.com)
Damage to our genomes triggers cellular senescence characterised by stable cell cycle arrest and a pro-inflammatory secretome that prevents the unrestricted growth of cells with pathological potential. (whiterose.ac.uk)
In this review, we will discuss the mechanisms according to which T2D induces cellular senescence and the role of type 2 diabetes-induced cellular senescence in the pathogenesis and progression of colorectal cancer. (frontiersin.org)
More recently, cellular senescence has been considered as an additional cause of age-related tumorigenesis. (frontiersin.org)
Senescence is a stress-response cellular state characterised by proliferative arrest but active metabolism ( 7 ). (frontiersin.org)
However, cellular senescence is not exclusive to ageing. (frontiersin.org)
Age-related and metabolic diseases such as type 2 diabetes (T2D) represent a source of cellular stress due to their disruptive effect on normal physiological processes and, therefore, can induce premature senescence ( 10 ). (frontiersin.org)
Keywords: SARS-CoV-2, cellular senescence, angiotensin II, prognosis, crucial illness, immune checkpoint inhibitors Introduction High transmissibility, asymptomatic service providers, and the absence of herd immunity have contributed to the quick worldwide spread of COVID-19 disease (1, 2). (ubatubasat.com)
Boosting these levels has in turn demonstrated significant benefits in staving off cellular senescence. (decadethirty.com)
Although much should be achieved for completely understanding the biological basis of aging, cellular senescence is now believed to mainly contribute to organismal aging via two independent, yet not mutually exclusive mechanisms: on the one hand, senescence of stem cells leads to exhaustion of stem cells and thus decreases tissue regeneration. (molcells.org)
RNA-Seq analysis of primary oligodendrocytes and microglia cells uncovers gene expression signatures associated with activated stress responses and increased post mitotic cellular senescence (PoMiCS) which was confirmed by elevated senescence-associated β-galactosidase activity and SASP gene expression profile. (biomedcentral.com)
Here, we investigated bleomycin-induced lung injury responses in young and aged mice at single-cell resolution to gain insights into the cellular and molecular contributions of aging to fibrosis. (researchgate.net)
At the cellular level, the mesenchymal stem cell pool in the bone marrow niche shows a biased differentiation into adipogenesis at the cost of osteogenesis. (biomedcentral.com)
We applied functional pathway analysis to show that HPV-positive cancers are characterised by perturbations of numerous cellular processes, predominantly in those linked to the cell cycle, mitosis, cytokine and immune cell signalling. (fortuneonline.org)
In response to this challenge, the International Society for Cellular Therapy formulated minimal criteria for defining MSCs in order to create a broader consensus for more uniform characterization of these cells (Dominici et al. (pdffox.com)
Our Cellular Senescence Flow Cytometry Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. (cellbiolabs.com)

Here, we show that nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme of the NAD + salvage pathway, governs the proinflammatory SASP independent of senescence-associated growth arrest. (nature.com)
Fig. 5: AMPK signalling mediates the proinflammatory SASP induced by NAD + metabolism. (nature.com)
On the other hand, etoposide treatment reliably induces DNA damage-related senescence in human articular chondrocytes evidenced by loss of proliferative capacity, DNA damage accumulation, and expression of some SASP components. (aging-us.com)
Doxo is able to trigger CFs senescence, as evidenced by an increase of Î³-H2A.X, p53, p21, and SA-ß-gal, and changes in the SASP profile. (bvsalud.org)
In addition, IL-1ß also triggered CFs senescence, as evidenced by the increase of Î³-H2A.X, p53, p21, SA-ß-gal activity, and SASP. (bvsalud.org)
The implication of ROCK 2 as a potential senotherapeutic target via the suppression of the harmful effects of the SASP: Do senescent cancer cells really engulf the other cells? (phiab.com)
ROCK2) have the potential to be developed into semomorphic drugs as they change SASP composition and reduce senescence cell secretory activity. (phiab.com)

Depending on the severity of the DNA damage, the cells may no longer be able to undergo repair and either go through apoptosis or cell senescence. (wikipedia.org)
We are investigating the role of Gli2 in protecting human keratinocytes from UVB induced apoptosis as well as its role in cell ploidy. (qmul.ac.uk)

We have made stable Gli1 and Gli2 expressing cell lines as well as PTCH knockdowns (in both N/TERT-1 and NEB-1) keratinocytes and these are being used as in vitro models to investigate the HH pathway in skin cancer and in conjunction with BCC derived stromal cells to develop novel in vitro models of BCC as alternatives to animals. (qmul.ac.uk)
PAI-1 expression is each necessary and enough to drive senescence in vitro downstream of p53,Circulation. (adenosine-receptor.com)
In vitro experiments show DNA damage induced by purified recombinant typhoid toxin or infection by toxigenic Salmonella elicited DNA damage responses and cell-cycle arrest, which was coincident with increased expression and secretion of APOC3 and LYZ. (whiterose.ac.uk)
MSC use frequently requires in vitro expansion, thus exposing cells to replicative senescence. (encyclopedia.pub)
However, insufficient number of cells and lack of regenerative properties during in vitro expansion still limit the clinical applicability of MSC therapies. (biomedcentral.com)
Most significant, concurrent activation of each RAS and PIK3CA/AKT impairs RAS-induced senescence, both in vitro and in vivo. (narturalproducts.com)
The latter encompass human skin regenerated on immune deficient mice as well as organotypic constructs with epithelial and stromal cells embedded within architecturally faithful mesenchyma in vitro. (stanford.edu)

This process is known as "replicative senescence", or the Hayflick limit. (wikipedia.org)
Mechanistically, replicative senescence can be triggered by a DNA damage response due to the shortening of telomeres. (wikipedia.org)
This induces replicative senescence. (wikipedia.org)
out there in PMC 2014 November 19.Boe et al.Pageand PAI-1-deficient murine embryonic fibroblasts are resistant to replicative senescence.25, 26 Nevertheless, really little is identified regarding the part of PAI-1 in senescence in vivo. (adenosine-receptor.com)

Notch 1 is expressed on thymocytes, bone marrow hematopoietic stem cells, T and NK cells. (thermofisher.com)
Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
Embryonic stem cells isolation primarily requires the consent of donors and can include the killing of fertilized eggs. (techscience.com)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)
We demonstrate that both, adipocyte- and iPSC-derived mesenchymal stem cells can guide this process. (regenhu.com)
Our tissue engineering research focuses on repairing diseased or damaged tissues, incorporating the areas of biomaterials, stem cells, computer modelling and molecular biology. (edu.au)
This review will focus on the most recent/relevant molecular findings driving this functional impairment of mesenchymal stem cells in the aging process. (biomedcentral.com)
Mesenchymal stem cells (MSCs), which are activated by secreted, active transforming growth factor beta 1 (TGFβ) to migrate to bone-resorptive sites and differentiate into osteoblasts (see below). (biomedcentral.com)
Human mesenchymal stem cells (MSCs) are therapeutic for clinical applications because of their excellent immunomodulatory and multiple lineage differentiation abilities at tissue injury sites. (biomedcentral.com)
MSCs have limited pluripotency and capacity for cell differentiation compared to embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). (biomedcentral.com)
After implantation, self-renewal and proliferative cytotrophoblasts appear in the early first trimester (6-9 weeks) and can help identify trophoblast stem cells (TSCs) [ 13 ]. (biomedcentral.com)
Using computational predictions, we revealed that HPV-positive cervical cancers are regulated by transcription factors including, SOX2, E2F, NANOG, OCT4, and MYC, which control various processes such as the renewal of cancer stem cells, and the proliferation and differentiation of tumour cells. (fortuneonline.org)
This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)

The phosphorylation cascade initiated by these two kinases causes the eventual arrest of the cell cycle. (wikipedia.org)
Importantly, we also present that activation of PIK3CA/AKT is deficient in its ability to drive two practical outputs with the senescence system which are central to senescence-mediated tumor suppression, namely upregulation of your senescence secretome and productive proliferation arrest. (narturalproducts.com)
In stratified epithelia proliferative basal cells adherent to the underlying basement membrane undergo cell cycle arrest then outward migration and terminal differentiation. (stanford.edu)

Senescent endothelial cells exhibit reduced eNOS activity and NO production,20, 21 and NO has been shown to be protective against the improvement of senescence, an effect which is D4 Receptor Inhibitor Source abrogated by L-NAME treatment.22, 23 Even so, the function of NO and L-NAME in vascular senescence in vivo is uncertain. (adenosine-receptor.com)
Therefore, along with validating TM5441 as a potential therapeutic, we also have demonstrated a function for L-NAME, NO, and PAI-1 in vascular senescence in vivo.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author CD30 Inhibitor Purity & Documentation ManuscriptMethodsTM5441 Activity and Specificity Assays The inhibitory activity and specificity of TM5441 (created in the United Centers for Sophisticated Research and Tr. (adenosine-receptor.com)
Due to the low abundance of MSCs in human adult tissues (about 1/10 6 cells in adult bone marrow and 1/10 3-4 cells in adipose tissue and umbilical cord) [ 16 ] , frequently ex-vivo expansion precedes therapeutic administration, to obtain a clinically relevant number of cells. (encyclopedia.pub)
To affirm this in vivo, in mice haboring activated RAS and activated PIK3CA/AKT signaling, the potent mTOR inhibitor, rapamycin, reactivated RAS-senescence. (narturalproducts.com)

This study used etoposide, to induce DNA damage-related senescence or chronic exposure to IL-1β to entail inflammation-related senescence in human OA chondrocytes. (aging-us.com)
Wu J, Li Q, Fu X. Fusobacterium nucleatum contributes to the carcinogenesis of colorectal cancer by inducing inflammation and suppressing host immunity. (exp-oncology.com.ua)
The persistent induction of senescence by pathogens, mediated directly through virulence determinants or indirectly through inflammation and chronic infection, also contributes to age-related pathologies such as cancer. (whiterose.ac.uk)
However, chronic inflammation can also elicit this response by prolonged activation of T cell receptors (TCRs) (14). (ubatubasat.com)

This pitfall may be due to the lack of understanding of the mechanisms underlying chondrocyte senescence. (aging-us.com)
It is therefore of critical importance to unveil the underlying mechanisms of MSC senescence and to define shared methods to assess MSC aging status. (encyclopedia.pub)
ABDULKADIR, RR, ALWJWAJ, M, RAKKAR, K and OTHMAN, OA, Outgrowth Endothelial Cell Conditioned Medium Negates TNF-alpha-Evoked Cerebral Barrier Damage: A Reverse Translational Research to Explore Mechanisms Stem Cell Reviews and Reports. (nottingham.ac.uk)
The pathogenic mechanisms involve a wide range of cells and soluble factors. (mdpi.com)
Epigenetic control of gene expression lasts through multiple cell divisions without alterations in primary DNA sequence and can occur via mechanisms that include histone modification and DNA methylation. (stanford.edu)

In their experiments during the early 1960s, Leonard Hayflick and Paul Moorhead found that normal human fetal fibroblasts in culture reach a maximum of approximately 50 cell population doublings before becoming senescent. (wikipedia.org)
Cardiac fibroblasts (CFs) undergo senescence in reaction to different stressors, leading to a poor prognosis of cardiac disease. (bvsalud.org)
In fact, several studies have shown that T2D induces senescence in multiple types of cells, including fibroblasts and endothelial cells ( 11 , 12 ). (frontiersin.org)
They include endothelial cells, fibroblasts, and keratinocytes. (frontiersin.org)

Indeed, SARS-CoV-2 has been associated with upregulation of ANG II, a molecule previously Pitavastatin Lactone shown to promote senescence in vascular easy muscle mass cells (VSMCs) and endothelial cells (ECs) (24C26). (ubatubasat.com)
Whilst activated RAS inhibits mTOR action to upregulate autophagy and promote senescence , activated AKT1 was in a position to activate mTOR even while in the presence of activated RAS, probable explaining the potential of mAKT1 to inhibit RASG12V-induced autophagy. (narturalproducts.com)

The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)
Interstitial microvoids enhanced differentiation of multiple stem/progenitor cells (vascular, mesenchymal, neural), otherwise not possible with conventional bulk hydrogels. (regenhu.com)
Mesenchymal cells in the lung are crucial during development, but also contribute to the pathogenesis of fibrotic disorders, including idiopathic pulmonary fibrosis (IPF), the most common and deadly form of fibrotic interstitial lung diseases. (researchgate.net)
Mesenchymal stem/stromal cells (MSCs) are a reservoir for tissue homeostasis and repair that age during organismal aging. (encyclopedia.pub)
In addition to their stem/progenitor properties, mesenchymal stromal cells (MSCs) possess broad immunoregulatory properties that are being investigated for potential clinical application in treating immune-based disorders. (pdffox.com)
Introduction Mesenchymal stromal cells (MSCs) are adult, fibroblast-like multipotent cells characterized by the ability to differentiate into tissues of mesodermal origin, such as adipocytes, chondroblasts, and osteoblasts (Friedenstein et al. (pdffox.com)

The current study was designed to test the hypothesis that resolvin E1 (RvE1) and overexpression of the receptor for RvE1 (ERV1) will prevent and/or reverse tumor generation in a gain-of-function mouse model of tumor seeding with lung cancer cells. (bvsalud.org)
There is an urgent need to better understand the biology of the tumor, including the diversity of cell types of which it is composed, and their role , Isabelle Le Roux explains. (institutducerveau-icm.org)
In that case, senescence contributes to the body's anti-tumor response. (institutducerveau-icm.org)
In the short term, the secretome is involved in recruiting immune cells to eliminate tumor cells , Isabelle Le Roux explains. (institutducerveau-icm.org)
In mice, suppressing a part of the senescent tumor cells made it possible to modify the immune activity within the tumor and extend the animal's lifespan. (institutducerveau-icm.org)
One strategy to increase cure rates is the identification of patient-specific drug responses in tissue models that mimic the interaction between patient cancer cells and tumor environment. (regenhu.com)
A gelatin-methacrylate/fibrin-based matrix containing multiple cell types mimics the tumor-microenvironment that promotes spontaneous micro-vessel formation by embedded endothelial cells. (regenhu.com)
We demonstrate that micro-vessels are attracted by and grow into tumor spheroids and that neuroblastoma cells invade the tumor-environment as soon as the spheroids disrupt. (regenhu.com)
Typically tumor cells secrete a complex milieu of growth factors, cytokines and chemokines, some of which are pro-tumorogenic [ 7 , 8 , 9 ]. (iospress.com)
Distinct functions of macrophage-derived and cancer cell-derived cathepsin Z combine to promote tumor malignancy via interactions with the extracellular matrix. (uni-freiburg.de)

Alzheimer's "patient-derived directly induced neurons exhibit strong transcriptomic, epigenetic, and molecular biomarker signatures, indicating a specific human neuronal senescence-like state," according to the researchers. (scienceboard.net)
A proteomic atlas of senescence-associated secretomes for aging biomarker development. (nih.gov)
PAI-1 is recognized as a marker of senescence and is a crucial member of a group of proteins collectively generally known as the senescence-messaging secretome (SMS).24 However, it is actually likely that PAI-1 just isn't just a biomarker of senescence, but as an alternative may perhaps be a important driver of this process. (adenosine-receptor.com)

The involvement of the NLRP3 inflammasome/interleukin-1 receptor (IL-1R) signaling pathway on CFs senescence was studied using an NLRP3 inhibitor (MCC950) and an endogenous IL-1R antagonist (IR1A). (bvsalud.org)

Lastly, we will explore the current therapeutic approaches and challenges in targeting senescence. (frontiersin.org)
Conversely, brown adipose tissue (BAT) and browning of WAT represent potential therapeutic approaches, since dysfunctional white adipocyte-induced lipid overspill can be halted by BAT/browning-mediated oxidative anti-lipotoxic effects. (springer.com)

Abstract In living tissues, cells express their functions following complex signals from their surrounding microenvironment. (regenhu.com)

The physiological importance for cell senescence has been attributed to prevention of carcinogenesis, and more recently, aging, development, and tissue repair. (wikipedia.org)
However, this results in a false positive for cells that naturally have these two proteins such as maturing tissue macrophages with senescence-associated beta-galactosidase and T-cells with p16Ink4A. (wikipedia.org)
Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
Bioprinted channels are coated with endothelial cells post printing to form a dense vessel - tissue barrier. (regenhu.com)
The tissue model thereby mimics structure and function of human soft tissue with endothelial cell-coated larger vessels for perfusion and micro-vessel networks within the hydrogel-matrix. (regenhu.com)
This pressing need has led to the rise of 'tissue engineering and regenerative medicine', a multidisclinary field which aims to induce the body's natural regenerative abilities and produce functional substitutes of biological tissue for clinical use. (edu.au)
The fundamental concept combines various tissue engineering elements, most often a scaffold as a supporting matrix in combination with living cells and/or bioactive molecules, to form a tissue engineering construct that repairs or regenerates the diseased or damaged tissue or organ. (edu.au)
Tissue engineering and regenerative medicine can provide a novel treatment regime based on the use of synthetic biomaterials, which may be constructed into three-dimensional implants and combined with biologics (such as cells and/or bioactive molecules). (edu.au)
These cells possess both differentiation plasticity (stemness) and tissue supportive functions (stromalness) that can coexist and overlap, with differences depending on tissue source, donor characteristics, culture conditions and delivery strategies, leading to alternative best fittings for the term "stem" or "stromal" [ 7 ] . (encyclopedia.pub)
During aging of the organism, MSCs also age, and this implies an impairment of stem cell functions contributing to the progressive decrease in tissue maintenance and repair, a characteristic of the aging process. (encyclopedia.pub)
Bone is a very dynamic and heterogeneous tissue formed by two components which are in close relationship with each other during the bone remodeling process: extracellular matrix (ECM) and bone cells. (biomedcentral.com)
Following the expansion of stem cell therapy using autologous MSC for tissue regeneration [ 3 ]. (biomedcentral.com)
There is also evidence that the deleterious effects mediated by dysfunctional white adipocyte-induced lipid overspill can be halted by the pro-oxidative anti-lipotoxic effects mediated by brown adipose tissue (BAT) activation. (springer.com)
Our experimental focus is on the mammalian setting using multiomics, informatics, mouse genetics, human genetics, single cell studies, and new human tissue platforms. (stanford.edu)

MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)
Here, we demonstrated a preconditioning strategy with trophoblast stem cell-derived extracellular vesicles (TSC-EVs) to boost the proliferation and regenerative capacity of MSCs. (biomedcentral.com)

This cell surface receptor is involved in T cell lineage commitment, thymocyte development, and Th2 differentiation. (thermofisher.com)
Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
As proof-of-concept, EmVP was applied to create complex synthetic biology-inspired intercellular communication models, where adipocyte differentiation is regulated by optogenetic-engineered pancreatic cells. (regenhu.com)
We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)

were the first to assess nanoparticle-like exosomes released from trophoblasts and determine the functional activity of trophoblast exosomes in human uterine spiral artery remodeling by inducing vascular smooth muscle cell migration for successful pregnancy [ 16 ]. (biomedcentral.com)

Myelination and homeostatic maintenance of myelin sheaths critically depend on the function of both mature OLs and their progenitor cells (called OPCs or NG2-glia), a process coupled to high energy demand. (biomedcentral.com)
Dr Bayraktutan is the Chief investigator of the Dunhill Medical Trust EPC study investigating the specific effect of ageing on the circulatory level and functional aspects of endothelial progenitor cells as well as the diagnostic and prognostic value of endothelial progenitor cells in elderly patients with lacunar or cortical stroke. (nottingham.ac.uk)
Prognostic, Diagnostic and Therapeutic Potential of Endothelial Progenitor Cells for Patients with Ischaemic Stroke: hype or hope BIOCELL. (nottingham.ac.uk)
Outgrowth Endothelial Progenitor Cells Restore Cerebral Barrier Function Following Ischaemic Damage: the impact of nox2 inhibition European Journal of Neuroscience. (nottingham.ac.uk)

As a result, angiotensin II (ANG II) accumulates in endothelial cells (ECs), inducing vascular senescence with upregulation of interleukin-6 (IL-6) and reactive oxygen species (ROS), impairing both innate and adaptive immunity. (ubatubasat.com)
Indeed, as in avian influenza, the Pitavastatin Lactone upregulation of NK cell, and CTC exhaustion markers (EMs) has been observed (11). (ubatubasat.com)
We hypothesize that vascular senescence-mediated upregulation of IL-6 and ROS is responsible for both coagulation and immune dysfunction. (ubatubasat.com)
Especially, we show that inactivation of PTEN and activation of AKT is impaired in its skill to induce senescence, as recorded by several effectors of senescence, such as upregulation of p16, induction of DNA injury, recruitment of HIRA to PML bodies, formation of SAHF and upregulation of autophagy. (narturalproducts.com)
First, activated AKT1 reversed the upregulation of p16INK4a the original source induced by activated RAS. (narturalproducts.com)

The composition of this fluid is very variable in terms of both the quantity and type of cells present, and the soluble factors and cytokines present. (iospress.com)

Rutgers University researchers have discovered that the underproduction of immune cells called mucosal-associated invariant T cells contribute to Alzheimer's. (scienceboard.net)
The hepatocytes, endothelial and immune cells of the liver are aligned along this vasculature, and their spatial distribution along the liver zonation dictates their phenotypes and functions. (encyclopedia.pub)
Microbial sensing by toll-like receptors (TLR) on liver sinusoidal endothelial cells (LSECs) results in a CXCL9 chemokine gradient that attracts Kupffer cells closer to the periportal region to ensure immune surveillance and host defense from invading pathogens arriving through the portal vein circulation. (encyclopedia.pub)
This project sheds light on host innate immune responses activated by bacterial-induced DNA damage and highlights that virulence factors can be viewed as double-edged swords, which promote infection but may also alert host defence. (whiterose.ac.uk)
Immunotherapy involves harnessing the body's immune system or T cells to combat the disease by directly killing the infected cells. (edu.au)

Mutations in genes relating to genome maintenance has been linked with premature aging diseases, supporting the role of cell senescence in aging (see DNA damage theory of aging). (wikipedia.org)
The successive shortening of the chromosomal telomeres with each cell cycle is also believed to limit the number of divisions of the cell, contributing to aging. (wikipedia.org)
Initially identified during the normal aging of cells, it corresponds to their loss of ability to divide. (institutducerveau-icm.org)
Long considered a simple marker of aging, we now know that senescence occurs throughout life, especially in response to genotoxic stress - that is, an event that disrupts or damages DNA, such as chemotherapy ," says Alexa Saliou , a doctoral student in the team and co-first author of the article. (institutducerveau-icm.org)
Built upon scDiffCom, scAgeCom is an atlas of age-related cell-cell communication changes covering 23 mouse tissues from 58 single-cell RNA sequencing datasets from Tabula Muris Senis and the Calico murine aging cell atlas. (nature.com)
Actually, stem cell exhaustion is considered one of the promoters of aging. (encyclopedia.pub)

The DNA damage response (DDR) arrests cell cycle progression until DNA damage, such as double-strand breaks (DSBs), are repaired. (wikipedia.org)
The researchers wondered whether there was senescence in glioblastoma and, if so, what role it might play in the cancer progression. (institutducerveau-icm.org)
Here, we draw upon studies that have shed light on the initiation and progression of islet autoimmunity from the point of view of the beta cell. (springer.com)

The blood microvascular endothelium consists of a heterogeneous population of cells with regionally distinct morphologies and transcriptional signatures in different tissues and organs. (researchgate.net)
We aim to gain a better understanding of the relationship between T cell functions and their physical microenvironment by 3D printing hydrogels as a model for the soft tissues and organs in our bodies. (edu.au)
Skin malignancies, including epidermal squamous cell carcinoma (SCC), alone account for nearly as many cancers as all other tissues combined. (stanford.edu)

Secretomes are released in response to the surrounding microenvironment. (techscience.com)
Through them, the cells modify their microenvironment and the behavior of neighboring cells. (frontiersin.org)

Second, the team looked at senescence markers and gene expression in post-mortem brains of 20 people with Alzheimer's and matched healthy controls. (scienceboard.net)

In this way, senescence can be considered a powerful innate defence against cancer and viral infection. (whiterose.ac.uk)
This review highlights the dichotomous role of senescence in infection: an innate defence that is exploited by pathogens to cause disease. (whiterose.ac.uk)

The researchers then defined a characteristic signature of senescence based on the expression of 31 genes in mice and ensured that it was identical in humans. (institutducerveau-icm.org)
Systemic induction of senescence in young mice after single heterochronic blood exchange. (nih.gov)
Within this study, we show that L-NAME remedy as well as the subsequent loss of NO production induces vascular senescence in wild-type (WT) mice, and that therapy using the PAI-1 antagonist TM5441 is protective against this senescence. (adenosine-receptor.com)

IL-17 and immunologically induced senescence regulate response to injury in osteoarthritis. (nih.gov)

Transcriptome data from isolated, primary OLs and microglia cells were explored by in silico pathway analysis and validated by complementary molecular approaches. (biomedcentral.com)
Molecular pathogenesis of acetaminophen-induced liver injury and its treatment options[J]. Journal of Zhejiang University Science B, 2022, 23(4): 265-285. (zju.edu.cn)

The EV composition depends on the producer cell type and its physiological conditions. (frontiersin.org)

Different approaches to evaluate the future of biomaterials and stem cell properties have been developed. (techscience.com)

Chronic exposure to IL-1β induces only partial expression of senescence markers and does not allow us to conclude on its ability to induce senescence in chondrocytes. (aging-us.com)

When cells enter senescence, they secrete various molecules. (institutducerveau-icm.org)

However, the discovery of senescence-like features in terminally differentiated cells, including neurons, has challenged the assumption that the phenomenon only applies to proliferating cells. (scienceboard.net)

CONCLUSION: These data show the anti-senescent role of RvE1 in Doxo-induced CFs senescence, which could be mediated by reducing IL-1ß secretion. (bvsalud.org)
In Basal Cell Carcinoma we are investigating the role of paracrine Hedgehog (HH) signalling between the stroma and epithelium and the role of oncogene induced senescence (OIS). (qmul.ac.uk)
We are also investigating the role of oxidative stress, Reactive Oxygen Species (ROS) and Dihydrotestosterone (DHT) on balding hair follicle senescence and the role of TGF-β. (qmul.ac.uk)
and CD8 + T cells play a major role as beta cell killers [ 6 , 7 ]. (springer.com)
For example, SARS-CoV-2 gains access to host cells via angiotensin-converting enzyme-2 (ACE-2) associated with the renin-angiotensin system (RAS), which, aside from regulating arterial blood pressure, plays a major role in immunity (15). (ubatubasat.com)
In the present work, we have isolated and characterized the extracellular vesicles from human AD-MSC to investigate their role in the chondroprotective actions of these cells. (karger.com)
Our findings demonstrate an essential role of IKK/NF-κB signaling in mature, post-mitotic OLs in regulating stress-induced senescence in these cells. (biomedcentral.com)
We employed cell proliferation analyses such as CCK8 and BrdU assays to determine the proliferation-promoting role of TSC-EVs on MSCs. (biomedcentral.com)
A recent study supports the pivotal role of trophoblast exosomes in that extracellular vesicles (EVs) derived from TSCs promote fertility by regulating endometrial cell receptivity [ 17 ]. (biomedcentral.com)
The adaptor protein Grb2 is able to enhance the activity of the cytoplasmic tyrosine kinase Btk through a novel mechanism, revealing a new role for Grb2 in B-cell signaling. (elifesciences.org)

Alzheimer's patients have significantly higher proportions of neurons that express markers of age-related deterioration, according to the research team's paper published December 1 in the journal Cell Stem Cell . (scienceboard.net)
First, the researchers took skin samples from people with Alzheimer's and converted the cells into neurons in the lab. (scienceboard.net)
Through the analyses, the researchers showed that the brains of Alzheimer's patients have significantly higher proportions of neurons that express senescence markers, and that their distribution indicates bystander effects. (scienceboard.net)
Single-cell transcriptomics showed that "senescent-like neurons face oncogenic challenges and metabolic dysfunction as well as display a pro-inflammatory signature. (scienceboard.net)
The researchers also performed integrative profiling of the inflammatory secretome of the induced neurons and patient cerebral spinal fluid. (scienceboard.net)

HSC, hepatic stellate cell. (encyclopedia.pub)
APAP overdose can induce hepatic toxicity, known as acetaminophen -induced liver injury (AILI). (zju.edu.cn)

Theoretically, it is possible upon the discovery of the exact mechanism of biological immortality to genetically engineer cells with the same capability. (wikipedia.org)

Doxorubicin (Doxo) is an antineoplastic drug with strong cardiotoxic effects, which induces IL-1ß secretion and thus, triggers a potent pro-inflammatory response. (bvsalud.org)

Normally, cell senescence is reached through a combination of a variety of factors (i.e., both telomere shortening and oxidative stress). (wikipedia.org)
Oligodendrocytes (OL) are responsible for homeostasis and maintenance of the myelin sheaths, which is a complex and highly energy demanding process sensitizing these cells to metabolic, oxidative and other forms of stress. (biomedcentral.com)

Hence, developing senolytic/semomorphic drugs, targeting senescence cells, are important to improve cancer treatment outcome. (phiab.com)

They are capable of secreting a broad range of biomolecules such as proteins, nucleic acids, exosomes, microRNAs, and membrane vesicles, collectively known as secretomes. (techscience.com)
The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. (frontiersin.org)
The exosome vesicles are a type of extracellular vesicles (EV), which are defined as lipid-bilayer spheroid structures, without replicating capacity, that are released from cells, including both prokaryotes and eukaryotes. (frontiersin.org)

Anatomy17

Organisms11

Diseases4

Chemicals and Drugs45

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes45

Disciplines and Occupations3

Information Science4

Secretory phenotype7

Phenotype6

Senescent cells25

Cellular35

SASP7

Apoptosis2

Vitro7

Replicative senescence4

Stem Cells14

Arrest3

Vivo4

Inflammation4

Mechanisms5

Fibroblasts4

Promote senescence2

Mesenchymal6

Tumor10

Biomarker3

Signaling pathway1

Therapeutic approaches2

Abstract1

Tissue15

Regenerative3

Differentiation5

Vascular smooth1

Progenitor cells4

Upregulation5

Cytokines1

Immune5

Aging6

Progression3

Tissues3

Microenvironment2

Gene expression1

Innate2

Mice3

Osteoarthritis1

Molecular2

Physiological1

Approaches1

Chondrocytes1

Secrete various1

Terminally differentiated1

Role10

Neurons5

Hepatic2

Biological1

Inflammatory1

Oxidative2

Senolytic1

Vesicles3