Search molecular peptide nucleic acids. Medical search. Frequent questions

It enables the synthesis of proteins with more than the naturally-encoded 20 amino acids, and provides insight into how nucleic acids form duplex structures, how proteins interact with nucleic acids, and how alternative genetic systems might appear in non-terran life. (wikipedia.org)
Molecules directly encoded by the genome (e.g. nucleic acids, proteins and peptides derived from proteins by cleavage) are not as a rule included in ChEBI. (obofoundry.org)
communicating and validating the models resulting from 3D structural studies on peptides, proteins, nucleic acids, and other molecules that act as ligands and co-factors. (europa.eu)
Minimizing the need for dramatic course reorganization, the structure, reactivity, and chemical biology applications of classes of biological monomers and polymers have been integrated into introductory organic chemistry courses through three series of semester-long weekly assignments that explored (a) Carbohydrates and Oligosaccharides, (b) Amino Acids, Peptides, and Proteins, and (c) Nucleosides, Nucleotides, and Nucleic Acids. (ed.gov)
A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. (lookformedical.com)
Protein cleavage is inhibited where the proteins, or nucleic acids and protein, contact each other. (lookformedical.com)
In addition, the molecular function of some of the described proteins suggests a central role for SVEVs in the cytotoxicity of the snake venom and sheds new light in the envenomation process. (nature.com)
After analysis of deduced amino acid sequences from cloned cDNAs of dipeptidyl peptidase IV (DPP IV) and aminopeptidase A (APA), it was noticed that DPP IV and APA are type II membrane proteins that have one transmembrane domain near the N-terminus. (nature.com)
Produced by a variety of cell types, extracellular vesicles (EVs) are able to transfer lipids, nucleic acids and proteins to recipient cells. (nature.com)
In addition to the well-established sense-antisense complementarity abundantly present in the nucleic acid world and serving as a basic principle of the specific double-helical structure of DNA, production of mRNA, and genetic code-based biosynthesis of proteins, sense-antisense complementarity is also present in proteins, where sense and antisense peptides were shown to interact with each other with increased probability. (usf.edu)
In proteins, the complementarity between sense and antisense peptides depends on a specific hydropathic pattern, where codons for hydrophilic and hydrophobic amino acids in a sense peptide are complemented by the codons for hydrophobic and hydrophilic amino acids in its antisense counterpart. (usf.edu)
These proteins show similar domain architectures as known organelle-targeted expression regulators of the octotrico peptide repeat type in algae and plants. (frontiersin.org)
Different surface coatings and chemical properties give each type of bead its own binding properties, which can be used for the magnetic separation (separation and purification) of nucleic acids, proteins or other biomolecules in an easy, effective and scalable manner. (aisenbio.com)
In molecular biology, magnetic beads provide a simple and reliable method to purify various types of biomolecules, including genomic DNA, plasmids, mitochondrial DNA, RNA and proteins. (aisenbio.com)
1) Binding biotinylated ligands, such as proteins, nucleic acids and peptides. (aisenbio.com)
This method is used not only for the analysis and purification of very large molecules, such as proteins and nucleic acids, but also for easier pregnant molecules, including pregnant sugars, amino acids, peptides, nucleotides, and simple ions. (pasteurlab.ir)
Proteins are composed of covalent amino acids and have different sizes and electrical loads, so they can be isolated electrochemically. (pasteurlab.ir)

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
Nucleic acid sequences involved in regulating the expression of genes. (lookformedical.com)
Recently it has become possible to accurately predict spectra from peptide sequences 14 , 15 , but direct searching of single-injection DIA data has remained problematic, in part due to the false discovery rate (FDR) correction required when considering all possible tryptic peptides in a FASTA database. (nature.com)
somewhat similar sequences of about 110 amino acids each. (wikipedia.org)
A group of scientists at the Wyss Institute for Biologically Inspired Engineering at Harvard University and MIT are convinced that automated machine learning (autoML) is going to revolutionize biology by removing many of the technical barriers to using computational models to answer fundamental questions about sequences of nucleic acids, peptides, and glycans. (bio-itworld.com)
Serology in the Digital Age: Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays. (cdc.gov)
The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)

MR contrast agent composed of cholesterol and peptide nucleic acids: Design, synthesis and cellular uptake. (mpg.de)
Synthesis and cellular uptake of a MR contrast agent coupled to an antisense peptide nucleic acid - cell- penetrating peptide conjugate. (mpg.de)
This course evolves around the synthesis of the naturally occurring biopolymers: carbohydrates, peptides, nucleic acids and hybrids thereof. (universiteitleiden.nl)
Their binding to DNA inhibits synthesis of nucleic acids. (lookformedical.com)
When a defined protein/peptide (or combinations thereof) control and define the synthesis of an inorganic nanoparticle, the result is a cloneable NanoParticle (cNP). (rsc.org)
These fatty acids are transported to the liver, where they are used for triglyceride synthesis and are exported as very-low-density lipoprotein (VLDL), which is elevated in these patients. (medscape.com)
Paradoxically, even in the face of hypoglycemia, patients with glycogen-storage disease I do not develop significant ketosis because the abundance of acetyl coenzyme A (CoA) derived from glycolysis activates the acetyl CoA carboxylase enzyme that produces malonyl CoA in the first step of fatty acid synthesis. (medscape.com)

Benner and his colleagues have since developed a six-letter artificially expanded genetic information system called Artificially Expanded Genetic Information System (AEGIS) which includes two additional nonstandard nucleotides (Z and P) in addition to the four standard nucleotides (G, A, C, and T). AEGIS has its own supporting molecular biology. (wikipedia.org)
The immense increase in the intracellular phosphorylated intermediate compounds of glycolysis concurrently inhibits rephosphorylation of adenine nucleotides, activating the nucleic acid degradation pathway and resulting in increased uric acid, the end product. (medscape.com)

Microbiome-derived molecules comprising nucleic acids, (poly)peptides and metabolites are present at high levels in the gut but have so far eluded systematic study. (fnr.lu)

Covalent Pyrenebutyrate-Cell Penetrating Peptide Conjugates: Enhanced Direct Membrane Translocation of Coupled Molecular Imaging Agents. (mpg.de)
Context Dependent Effects of Chimeric Peptide Morpholino Conjugates Contribute to Dystrophin Exon-skipping Efficiency. (ox.ac.uk)
Four additional chimeric peptide-PMO conjugates including newly identified peptide 9 (B-9-PMO and 9-B-PMO) and control peptide 3 (B-3-PMO and 3-B-PMO) were tested in mdx mice. (ox.ac.uk)
Immunohistochemical staining, RT-PCR and western blot results indicated that B-9-PMO induced significantly higher level of exon skipping and dystrophin restoration than its counterpart (9-B-PMO), further corroborating the notion that the activity of chimeric peptide-PMO conjugates is dependent on relative position of the tissue-targeting peptide motif within the chimeric peptide with respect to PMOs. (ox.ac.uk)
Surprisingly, further evidence showed that the uptake of chimeric peptide-PMO conjugates of both orientations (B-MSP-PMO and MSP-B-PMO) was ATP- and temperature-dependent and also partially mediated by heparan sulfate proteoglycans (HSPG), indicating that endocytosis is likely the main uptake pathway for both chimeric peptide-PMO conjugates. (ox.ac.uk)
Peptide nucleic acids and their conjugates to peptides can self-assemble and generate complex architectures. (bvsalud.org)

Finally, this work developed a method of using translation occluding Peptide Nucleic Acids (PNA) to inhibit Rickettsial protein expression. (umaryland.edu)
While this method is often prohibitively expensive because it requires enough instrument time and protein content for multiple injections for each sample, the use of multiple GPF injections can be applied just to pooled samples to generate DIA-only chromatogram libraries that make it easier to detect peptides in single-injection DIA experiments 13 . (nature.com)
This ease of use makes it easy to automate and is ideal for a range of applications, including sample preparation for next-generation sequencing (NGS) and PCR, protein purification, molecular and immunodiagnosis, and even magnetic activated cell sorting (MACS) Wait. (aisenbio.com)
The presented data clearly demonstrate the existence of hot spots of aggregation located in different regions, mostly in the N-terminal domain (NTD) of the entire NPM1 protein, and provide a more comprehensive view of the molecular details potentially at the basis of NPMc+-dependent AML. (bvsalud.org)
We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
All recognized mutations for AD are associated with increased deposition of amyloid-beta (Abeta), a peptide fragment comprising 39-43 amino acids that derive from the catabolism of the amyloid precursor protein (APP) molecule. (medscape.com)
Goethe-Universita¨t, DE-60439 tein (I-LBP) and intestinal fatty acid-binding protein (I-FABP). (lu.se)

Cell-Penetrating Peptides and Peptide Nucleic Acid-Coupled MRI Contrast Agents: Evaluation of Cellular Delivery and Target Binding. (mpg.de)
We designed antimiRs against both miRNAs with a phosphorodiamidate morpholino oligonucleotide (PMO) chemistry conjugated to cell-penetrating peptides (CPPs) to improve delivery to affected tissues. (ox.ac.uk)
We have recently reported that cell-penetrating peptides (CPPs) and novel chimeric peptides containing CPP (referred as B peptide) and muscle-targeting peptide (referred as MSP) motifs significantly improve the systemic exon-skipping activity of morpholino phosphorodiamidate oligomers (PMOs) in dystrophin-deficient mdx mice. (ox.ac.uk)

By 1996 Benner joined the faculty at the University of Florida, as a professor in both chemistry and cell & molecular biology. (wikipedia.org)
In: Peptides 2008: Chemistry of Peptides in Life Science Technology and Medicine, 4-19-122, pp. 550 - 551 (Eds. (mpg.de)
No clinical assessment currently employs the basic phosphodiester chemistry found in natural nucleic acids, because of these and other known problems. (justia.com)
Bioconjugation via click chemistry is widely used in proteomics and nucleic acid research. (crbdiscovery.com)
For students with basic knowledge of organic chemistry (BSc in Chemistry, Molecular Science and Technology, Life Science and Technology or a related discipline). (universiteitleiden.nl)
Combine peptide/carbohydrate and nucleic acid chemistry to synthesize hybrid structures. (universiteitleiden.nl)
Our research blends chemistry with engineering, biophysics and pharmacology to, for example, study factors involved in the recognition of small-molecule targets by nucleic acid- or peptide-based receptors, design and fabricate implantable biosensing devices, develop metabolism-responsive drug delivery approaches, and produce diagnostic platforms for personalized health care. (hopkinsmedicine.org)
The course consists of a series of lectures that cover available databases relevant to chemistry, search strategies, and relevant search terms within chemistry. (lu.se)

In my group we use and develop bioinformatics tools for analysing molecular sequence data. (lu.se)

Beyond the bulk fluid assay and droplet devices, use of dsPNA probes may be advantageous in a wide variety of assays that employ homogenous nucleic acid hybridization. (psu.edu)
Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of mycobacterium immunogenum and DNA-FISH assay for analysis of pseudomonads in metalworking fluid s and sputum. (cdc.gov)
A sensitive and quantitative assay using peptide nucleic acid-mediated real-time PCR was developed for detecting FGFR3 mutations in the urine samples and evaluated as a molecular marker for detecting intravesical recurrence of NMIBC in patients undergoing transurethral resection of bladder tumor. (elsevierpure.com)

Benner attended Yale University, receiving his B.S./M.S. in molecular biophysics and biochemistry in 1976. (wikipedia.org)
While our canonical knowledge of biosignatures is rooted in Terran-based examples, agnostic approaches in astrobiology can cast a wider net, to search for signs of life that may not be based on Terran-like biochemistry. (frontiersin.org)
Thus, a robust search for present or past life on other planetary bodies will be facilitated by the development of analytical tools that enable the search for multiple types of biosignatures, whether they are based on familiar biochemistry or not. (frontiersin.org)

A structured classification of molecular entities of biological interest focusing on 'small' chemical compounds. (obofoundry.org)
A freely available dictionary of molecular entities focused on 'small' chemical compounds. (obofoundry.org)

Intracellular MR Contrast Agents with Enzymatically Cleavable Part for Molecular Imaging. (mpg.de)
Comparison of a lipid- vs. peptide-based delivery system for intracellular targeted MR imaging probes. (mpg.de)
Vectors based on reducible polycations facilitate intracellular release of nucleic acids. (ox.ac.uk)
BACKGROUND: Inefficient intracellular delivery of nucleic acids limits the therapeutic usefulness of synthetic vectors such as poly(L-lysine) (PLL)/DNA polyplexes. (ox.ac.uk)
This article reports on the characterisation of a new type of synthetic vector based on a linear reducible polycation (RPC) that can be cleaved by the intracellular environment to facilitate release of nucleic acids. (ox.ac.uk)
CONCLUSIONS: These results demonstrate that lipid-mediated activation of RPC-based polyplexes is a useful strategy to enhance intracellular delivery of nucleic acids and potentiate therapeutic activity. (ox.ac.uk)
I-FABP) and bovine heart fatty acid-binding pro- tissues and in intracellular as well as extracellular tein (H-FABP). (lu.se)

Modified nucleic acids such as peptide nucleic acid (PNA) offer advantages compared to conventional DNA probes allowing for faster hybridization, better stability and minimal sample preparation for direct detection of pathogens. (psu.edu)
Here, we compared the sensitivity and specificity of double stranded PNA probes (dsPNA) and PNA molecular beacons targeting E. coli and P. aeruginosa for direct detection of bacterial pathogens. (psu.edu)

Biocytin-based contrast agents for molecular imaging: an approach to developing new in vivo neuroanatomical tracers for MRI. (mpg.de)
This invention relates to compositions comprising a combination of a lipid and a therapeutic agent, particularly to lipid-nucleic acid compositions, for in vivo therapeutic use. (justia.com)
In the present study, the general mechanistic significance of the chimeric peptide configuration on the activity and tissue uptake of peptide conjugated PMOs in vivo was investigated. (ox.ac.uk)

Diverse strategies including decoy oligonucleotides peptide nucleic acids ribozymes small-interfering RNAs and low-molecular fat compounds have already been explored to inhibit specifically Sp1 appearance or activity for healing applications [8-14]. (colinsbraincancer.com)
The symptoms of LMW asthma are similar to those induced allergens like trimellitic anhydride (TMA), the relationship by high molecular weight (HMW) compounds. (cdc.gov)

N-terminal amino acid sequence analysis of the purified DPP IV revealed that the N-terminus is not processed. (nature.com)

A program for building, displaying and manipulating all kinds of crystal and molecular structures. (rcsb.org)

Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. (bvsalud.org)

Molecular Microbiology and Immunology. (umaryland.edu)

CyLoP-1: A novel cell penetrating peptide with remarkable cellular uptake for cytosolic targeting. (mpg.de)
Collectively, our data demonstrate that peptide orientation in chimeric peptides is an important parameter that determines cellular uptake and activity when conjugated directly to oligonucleotides. (ox.ac.uk)

Genome Sequence of a Candidate World Health Organization Reference Strain of Zika Virus for Nucleic Acid Testing. (cdc.gov)
To contribute to the hazard identification of low molecular weight (LMW) respiratory allergens, respiratory allergy induced by trimellitic anhydride (TMA) was characterized by whole genome analysis of lung tissue and blood proteomics in Brown Norway rats. (cdc.gov)

Methods for the preparation of a lipid-nucleic acid composition are provided. (justia.com)
According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or Polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. (justia.com)
Additionally, the invention provides methods of making the compositions, as well as methods of introducing the nucleic acids into cells using the compositions and treating disease conditions. (justia.com)
METHODS: RPCs of molecular weight (mwt) 45 and 187 kDa were prepared by oxidative polycondensation of the peptide Cys-Lys(10)-Cys and used to condense nucleic acids. (ox.ac.uk)
In order to characterise this syndrome better, facilitate the diagnosis, and provide targeted health care for affected individuals, we have studied 33 patients (32 new observations) with a pure 22q13 partial monosomy, using molecular and cytogenetic methods. (bmj.com)
METHODS: Research and review articles and preprints connecting EphA2 to different viruses have been searched through PubMed and the web. (bvsalud.org)

We demonstrate a library generation workflow that leverages fragmentation and retention time prediction to build libraries containing every peptide in a proteome, and then refines those libraries with empirical data. (nature.com)

Her work leverages molecular simulations to explore the structural properties of conducting polymers and bioinspired materials for applications in biosensors and bioelectronics. (kcl.ac.uk)

After completion of the cutting reaction, the remaining peptide fragments are analyzed by electrophoresis. (lookformedical.com)

I-LBP, ileal lipid-binding to the aqueous solubility of the fatty acid. (lu.se)

Here, we delve into the search for extraterrestrial chemical and morphological biosignatures and examine several possible approaches to agnostic life detection using mass spectrometry. (frontiersin.org)
Nucleic acid hybridization is at the core of many amplification-free molecular diagnostics and detection probe configuration is key to diagnostic performance. (psu.edu)
Only peptides present in the libraries can be detected, and the peptide detection reports must be corrected to limit the number of potential false discoveries 8 . (nature.com)
Gas-phase fractionation 12 (GPF) improves detection rates with these tools 10 by injecting the same sample multiple times with tiled precursor isolation windows, allowing each injection to have narrower windows (and thus fewer co-fragmented peptides) with the same instrument duty cycle. (nature.com)

The prognosis of lung carcinoma has changed since the discovery of molecular targets and their specific drugs. (bio-itworld.com)

Interrogating Enzymatic Reactions using Nucleic Acid Molecular Recognition and Assembly. (rsc.org)
Dr. Arroyo's research lab uses DNA as a molecular recognition tool, and integrates the DNA into electronic devices for biomedical sensing applications. (hopkinsmedicine.org)

As evidenced by the 1990 US patent, their potential in nucleic acid purification has been recognized. (aisenbio.com)

The latter vaccines may also be whole viruses whose nucleic acids have been modified. (bvsalud.org)

By providing mechanistic insights into the molecular basis of human-microbiome interactions, the project will generate essential new knowledge about causal relationships between the gut microbiome and the immune system. (fnr.lu)

17-19 Among these, the simulations by molecular dynamics (MD) simulations. (lu.se)

Interactive molecular display for research and educational uses. (rcsb.org)
Nucleic Acids Research. (lu.se)
Additionally, there is a brief project where participants conduct a preliminary search with an associated search strategy within their research field. (lu.se)

The molecular entities in question are either products of nature or synthetic products used to intervene in the processes of living organisms. (obofoundry.org)

In fact, manufacturers have now developed many commercial nucleic acid separation kits based on magnetic beads. (aisenbio.com)

The course also discusses the advantages and disadvantages of different search strategies, tools, and databases. (lu.se)

Bioconjugation is a descriptive term for the joining of two or more different molecular species by chemical or biological means, in which at least one of the molecular species is a biological macromolecule. (justia.com)
This definition broadly captures all biological systems on Earth (Terran life) as well as life forms with potentially differing underlying molecular frameworks. (frontiersin.org)
To this end database search engines were rapidly developed after the introduction of ionization techniques for biological mass spectrometry [1-5]. (lu.se)

When experiment-specific spectrum library generation is either impossible or impractical, as is frequently the case with non-model organisms, sequence variants, splice isoforms, or scarce sample quantities, software tools such as Pecan 10 and DIA-Umpire 11 can detect peptides from DIA experiments without a spectrum library by directly searching every peptide in FASTA databases. (nature.com)
However, even when using GPF, these tools still generally detect fewer peptides than library search engines, which can leverage previously acquired instrument-specific fragmentation and measured retention times. (nature.com)

Both modified and unmodified peptides have to be present in the sample to be detected. (lu.se)

This study demonstrates that these phylogenetically distinct peptides are also distinct in their antimicrobial mechanism of action and specificity for transmitted bacterial species. (umaryland.edu)

Severe hypoglycemia stimulates epinephrine secretion, which activates lipoprotein lipase and the release of free fatty acids. (medscape.com)
Because malonyl CoA inhibits transport of fatty acid into the mitochondrion, beta-oxidation of fatty acids for energy to support the hypoglycemic cells does not occur. (medscape.com)

Anatomy1

Diseases2

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes7

Information Science2

Health Care1

Proteins17

Sequences8

Synthesis7

Nucleotides2

Molecules1

Conjugates6

Protein8

Cell-penetrat3

Chemistry8

Sequence Data1

Assay3

Biochemistry3

Chemical compounds2

Intracellular7

Probes2

Vivo3

Compounds2

Amino acid seq1

Structures1

Antigens1

Immunology1

Cellular uptake2

Genome2

Methods6

Proteome1

Structural1

Electrophoresis1

Lipid1

Detection4

Targets1

Recognition2

Purification1

Viruses1

Mechanistic1

Simulations1

Research3

Synthetic1

Separation1

Advantages1

Biological3

Detect2

Present1

Distinct1

Fatty2