Analysis of plasmid, cosmid or genomic DNA in combination with various methods, e.g. (openwetware.org)
Chromosome3
Sequencing of six neighbouring EcoRI fragments of cosmid UKG041 from chromosome XI. (elsevierpure.com)
Starting with two cosmids within 3p21.1, D3S92 and D3S93, we have isolated two separate contigs of overlapping cosmids within 3p21.1, by screening a library of 5700 chromosome 3-specific cosmid clones. (elsevierpure.com)
These results represent a valuable transcriptional map of chromosome 11q13 that will help in the search for disease genes in this region. (ox.ac.uk)
Clones2
One hundred cosmid clones, each with ≈40-Kb DNA insert, were partially sequenced in both directions by using primers M13 and T7. (cdc.gov)
Twenty-four of these clones were purified, analyzed, and found to comprise two groups according to the reactivity of the recombinant proteins with LL sera and to the DNA restriction patterns of the recombinant plasmids and cosmids. (elsevierpure.com)
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Genes4
The nucleotide sequences were assembled with Sequencher 4.6 (Gene Codes Corp., Ann Arbor, MI, USA) and BLAST ( www.ncbi.nlm.nih.gov/BLAST ) homology searches identified 37 putative genes. (cdc.gov)
With Sean Brady, he developed a method to insert DNA directly from environmental DNA (eDNA) like soil, into cosmid libraries, search for biosynthetic gene clusters and identify the products of these genes. (wikipedia.org)
We have identified five new genes from these two contigs and there is evidence to suggest that several additional genes reside within these cosmid contigs. (elsevierpure.com)
Two adjacent genes, salA and salB, conferring beta-glucosidase activity to Escherichia coli, have been identified in a cosmid library of A. irakense DNA. (lookformedical.com)
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Library2
6 ). A cosmid DNA library was constructed by using pWEB kit (Epicenter Biotechnologies, Madison, WI, USA) by following protocols described elsewhere ( 7 ). (cdc.gov)
Screening of the Mycobacterium leprae cosmid library with pooled sera from lepromatous leprosy (LL) patients by a colony immunoblot technique resulted in the identification of about 100 colonies that produced immunologically reactive proteins. (elsevierpure.com)
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Putative1
Similarity searches and Southern blot analyses suggested that a putative tyrosinase an polysaccharide deacetylase gene define the ends of the cluster. (usda.gov)
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Categories1
Select up to three search categories and corresponding keywords using the fields to the right. (cdc.gov)
Analysis1
Diagnostic analysis of the Rubinstein-Taybi syndrome: five cosmids should be used for microdeletion detection and low number of protein truncating mutations. (medscape.com)
Results2
Use filters to refine the search results. (edu.au)
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Bacterial Artificial1
Die Analyse großer Cosmid- und BAC- (Bacterial Artificial Chromosome) Contigs, welche im Rahmen dieser Arbeit erstellt wurden und die mehr als 1 Mb sowohl des X- als auch des Y-Chromosoms des Platys X. maculatus abdecken, zeigte eine hohe Dichte von Retroelementen und anderen repetitiven Sequenzen, besonders im Bereich des dominanten Onkogens Xmrk und in einer durch das duplizierte Gen ps-criptY gekennzeichneten, Y-spezifischen Region. (uni-wuerzburg.de)
Single cosmid4
Bufton L, Bruns GA, Magenis RE, Tomar D, Shaw D, Brook D, Litt M , Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to chromosome 19. (coriell.org)
Buroker NE, Magenis RE, Weliky K, Bruns G, Litt M , Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q. (coriell.org)
A typical HTG record might consist of all the first-pass sequence data generated from a single cosmid, BAC, YAC, or P1 clone, which together make up more than 2 kb and contain one or more gaps. (nih.gov)
For example, although the majority of submissions represent a collection of unordered or ordered sequences derived from a single cosmid, BAC, or PAC clone, there have been cases where each individual sequence was submitted as phase 1, then updated to phase 2, then upon assembly updated to phase 3. (nih.gov)
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Artificial chromosomes1
FISH probes may be gene- or locus-specific -- such as cosmids or yeast artificial chromosomes (YACs) -- or may be probes that "paint" an entire chromosome (1,2). (nih.gov)
Probes1
Thus, when an investigator wishes to map a new human cDNA to its chromosomal location, genome experts such as NCHGR's Mihael Polymeropoulos advocate first searching the sequence databases (to confirm that the gene or a homolog hasn't already been mapped), then generating oligo probes that can be hybridized to a series of known YAC markers that have been developed to span the entire genome. (nih.gov)
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Gene4
18. Cystic fibrosis: diagnostic testing and the search for the gene. (nih.gov)
The nucleotide sequences were assembled with Sequencher 4.6 (Gene Codes Corp., Ann Arbor, MI, USA) and BLAST ( www.ncbi.nlm.nih.gov/BLAST ) homology searches identified 37 putative genes. (cdc.gov)
With Sean Brady, he developed a method to insert DNA directly from environmental DNA (eDNA) like soil, into cosmid libraries, search for biosynthetic gene clusters and identify the products of these genes. (wikipedia.org)
A DNA-stable isotope probing-based cosmid library enabled the retrieval of functional genes from an uncultivated organism capable of PCB metabolism and suggest dispersed dioxygenase gene organization in nature. (nih.gov)
Contigs2
Approximately 60 % of the expressed genes of the human genome is known to be associated with CpG islands, most of which are able to be detected with BssHII, EagI and SacII.To help isolate and identify genes, we have sought CpG-islands in the cosmid/PAC contigs with these diagnostic enzymes, assuming close clustering (within l kb) of two or more cutting sites as potential CpG islands. (nii.ac.jp)
Publications] Osoegawa, K.: 'An Integrated Map with Cosmid/PAC Contigs of 4-Mb Down Syndrome Critical Region' Genomics. (nii.ac.jp)
Sequence7
There is no standard nomenclature for the genetic maps, and if searching for a particular sequence, there is a 20 to 30 percent chance the clone is incorrect. (nih.gov)
This approach, designated as CpG-tagged sequence, is rapid and convenient for searching genes and will provide a scaffold for the construction of a transcription map. (nii.ac.jp)
In addition, we have established a 1200-kb sequence-ready contig consisting of 26 cosmids, eight BACs, and eight PACS that encompass this region. (ox.ac.uk)
A 31.8-kb cosmid clone, detected by aromatic dioxygenase primers, contained genes of biphenyl dioxygenase subunits bphAE, while the rest of the clone's sequence was similar to that of an unknown member of the Gammaproteobacteria. (nih.gov)
Sequence data in this division are available for BLAST homology searches against either the "htgs" database or the "month" database, which includes all new submissions for the prior month. (nih.gov)
As the genetic and physical map (and ultimately the sequence) for the human genome become more complete, it is increasingly likely that mapping by computer search will become routine, and band-region information will become available for all markers. (nih.gov)
Both systems allow searching of the Taxonomy database for names, and both link to the relevant sequence data. (nih.gov)
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Restriction1
The steps in the analysis are the development of maps: (1) physical, (2) restriction, (3) cosmid, and (4) linkage. (nih.gov)
Polymorphisms1
This was achieved by investigating 17 cosmids for microsatellite polymorphisms, which defined two novel polymorphisms at the VRF and A0138 loci, and utilizing these to characterize recombinants in MEN1 families. (ox.ac.uk)
Retrieval1
However, the Taxonomy Browser provides a hierarchical view of the classification (the best display for most casual users interested in exploring our classification), whereas Entrez Taxonomy provides a uniform indexing, search, and retrieval engine with a common mechanism for linking between the Taxonomy and other relevant Entrez databases. (nih.gov)
Genetic1
7. Physical and genetic analysis of cosmids from the vicinity of the cystic fibrosis locus. (nih.gov)
Terms1
This search feature obtains best-matches with the terms you choose, and shows an overall score based on the scientific rankings. (nih.gov)
Human2
Publications] Soeda, E.: 'Cosmid Assembly and Anchoring to Human Chromosome21' Genomics. (nii.ac.jp)
Tg19959 mice were constructed by injecting FVB x 129S6 F1 embryos with a cosmid insert containing human APP695 with two familial AD mutations (KM670/671NL and V717F), under the control of the hamster PrP promoter. (nih.gov)
Updates1
Would you like email updates of new search results? (nih.gov)
Results2
Use filters to refine the search results. (edu.au)
Results 1-1 of 1 (Search time: 0.002 seconds). (edu.au)
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History1
Clipboard, Search History, and several other advanced features are temporarily unavailable. (nih.gov)
Page1
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