• Mutations in the gene for the antennapedia homeodomain protein are associated with the conversion of antenna to leg or leg to antenna DROSOPHILA. (lookformedical.com)
  • Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL). (lookformedical.com)
  • Products of the adenovirus E1A gene can act synergistically with cAMP to activate transcription of several viral early genes and the cellular genes c-fos and jun-B. Transcription factor AP-1-binding activity is also induced by the combined action of E1A and cAMP. (princeton.edu)
  • These data suggest that the 243- and 289-amino acid E1A proteins both interact functionally with the cAMP signaling system to activate transcription of a cellular gene and AP-1-binding activity. (princeton.edu)
  • I have previously reported (Frisch, 1991) that adenovirus E1a is a tumor suppressor gene in certain human cell lines. (rupress.org)
  • The transforming gene products of these viruses, such as the E1A oncoproteins of adenovirus 5, led to the identification of cellular factor p60, known as cyclin A. This research was the first demonstration of a physical link between cellular transformation and the cell cycle, thereby paving the way for the melding of these two areas of research. (newswise.com)
  • The role of these host factors in virus infection was determined using gene silencing, identifying several host proteins involved in the SARS-CoV-2 replication cycle. (bvsalud.org)
  • The retinoblastoma (Rb) gene product forms a complex with the cellular transcription factor DRTF1, a property assumed to be important for mediating negative growth control because certain viral oncogenes, such as adenovirus E1a, prevent this interaction and mutant Rb alleles, which have lost the capacity to regulate growth, encode proteins that fail to associate with DRTF1. (ox.ac.uk)
  • Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)
  • In the present study, the hypothesis that adenoviral infection followed by persistent expression of its E1A gene is capable of producing epithelial-mesenchymal transformation was examined by investigating guinea pig PLECs that express adenoviral E1A. (ersjournals.com)
  • CBP (CREB (cAMP responsive element binding protein) binding protein (CREBBP)) and P300 (adenovirus E1A-associated 300 kDa protein) are two closely related histone acetyltransferases (HATs) that play a key role in the regulation of gene transcription. (ox.ac.uk)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
  • 1994. Expression of thyrotropin-releasing hormone receptors by adenovirus-mediated gene transfer reveals that thyrotropin-releasing hormone desensitization is cell specific. . (cornell.edu)
  • 1985. Transcription termination within the E1A gene of adenovirus induced by insertion of the mouse beta-major globin terminator element. . (cornell.edu)
  • These studies have generated adenoviruses with miRNA target sites placed in the 3'UTR of early gene products. (oncotarget.com)
  • In this work, we have studied the feasibility of providing tissue-specific selectivity to replication-competent adenoviruses through the regulation of the late structural protein fiber (L5 gene). (oncotarget.com)
  • In vivo , liver production of infective viral particles was highly impaired, similarly to that triggered by an adenovirus with miRNA target sites regulating the early E1A gene. (oncotarget.com)
  • Gene therapy using a recombinant adenovirus (Ad) encoding secretory human endostatin (Ad-Endo) has been demonstrated to be a promising antiangiogenesis and antitumor strategy of in animal models and clinical trials. (biomedcentral.com)
  • Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. (riken.jp)
  • Fukuda K., E1A, E1B double-restricted replicative adenovirus at low dose greatly augments tumor-specific suicide gene therapy for gallbladder cancer. (riken.jp)
  • Recombinant adenovirus (Adenovirus), a replication-defective adenoviral vector system, is widely used for gene delivery in most cell types. (9lb.cc)
  • Adenovirus packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro. (9lb.cc)
  • Genemedi has launched a comprehensive adenovirus production service, including a variety of different adenoviral expression systems used for gene over-expression and shRNA mediated gene down-regulation, which may also allow researchers to produce adenoviruses by themselves as they need. (9lb.cc)
  • 2. What is the role of the E1 Gene in adenoviruses? (9lb.cc)
  • Simply, the E1 gene products are early proteins that are transcribed in the early transcribed regions and required for proceeding subsequent steps in viral replication. (9lb.cc)
  • The E1 gene contains E1A and E1B, involved in the replication of adenovirus. (9lb.cc)
  • E1A is critical to start viral replication by promoting transcription from rep gene promoters, P5 and P19, and facilitate viral replication by activating the early adenovirus promoters. (9lb.cc)
  • Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. (lookformedical.com)
  • The proteins encoded by homeobox genes are called HOMEODOMAIN PROTEINS. (lookformedical.com)
  • In contrast, the 243-amino acid protein was a poor activator of the viral early genes E2 and E4 compared with the 289-amino acid protein. (princeton.edu)
  • Search for RefSeq genes (e.g. (vectorbuilder.kr)
  • The Conserved region 3 (CR3) portion of the human adenovirus (HAdV) 5 E1A protein functions as a potent transcriptional activator that induces expression of viral early genes during infection. (birmingham.ac.uk)
  • The transcription of protein-coding genes involves RNA polymerase II and a number of common factors (TFIIA, -B, -D, -E, -F and -H) that form functional preinitiation complexes (on promoters) via an ordered assembly pathway. (rockefeller.edu)
  • In order to produce a replication-deficient vector, the two early genes E1A and E1B of adenovirus were replaced with transgene (the insertion antigen of the target pathogen) expression cassette, thereby preventing the virus from replicating in the host cell. (medicaltrend.org)
  • In addition, by genetically depleting the E3 and E4 genes of the adenovirus vector, the host cell-mediated elimination of adenovirus-infected cells and the instability of the transgene can be prevented. (medicaltrend.org)
  • The adenovirus without E1/E3 can express all of the other genes in the viral backbone and hence induces immunogenic responses, while rAAV does not have any of the AAV genes, thus no immunogenicity from viral protein. (9lb.cc)
  • In humans, 57 adenovirus serotypes have been identified and further divided into 7 subgroups (A-G). Most adenovirus-based vaccines are prepared using adenovirus type 5 (Ad5). (medicaltrend.org)
  • Oncolytic adenoviruses hold great promise for cancer treatment since they can replicate and destroy cancer cells. (oncotarget.com)
  • The development of more potent oncolytic adenoviruses faces with a potential increase in associated-toxicities. (oncotarget.com)
  • To improve the safety of oncolytic adenoviruses a control of the viral tropism based on tissue-specific miRNA regulation has been exploited. (oncotarget.com)
  • Antiviral immune responses present a major hurdle to the efficacious use of oncolytic adenoviruses as cancer treatments. (aacrjournals.org)
  • In this study, we tested the hypothesis that tumor-infiltrating T cells could be more effectively activated and redirected by oncolytic adenoviruses that were armed with bispecific T-cell-engager (BiTE) antibodies. (aacrjournals.org)
  • The Rb protein therefore acts in trans to repress the transcriptional activity of DRTF1 whereas adenovirus E1a prevents this interaction and thus maintains DRTF1 in a constitutively active state. (ox.ac.uk)
  • Transcriptional repression mediated by the Rb protein and inactivation of repression by the E1a protein are likely to play an important role in mediating their biological effects. (ox.ac.uk)
  • Adenovirus Protein VII Condenses DNA, Represses Transcription, and Associates with Transcriptional Activator E1A , 78 Journal of Virology 6459 (2004) (with Yvette Osheim, Yuming Xue, Margaret Emanuel, Peter Lewis, Alex Bankovich, Ann Beyer & Daniel Engel). (northwestern.edu)
  • Our results identify a second independent binding site for pCAF in E1A and Suggest that it contributes to CR3 dependent transcriptional activation. (birmingham.ac.uk)
  • A cellular transcriptional coactivator that was originally identified by its requirement for the stable assembly IMMEDIATE-EARLY PROTEINS of the HERPES SIMPLEX VIRUS. (rush.edu)
  • E1a induces the expression of epithelial characteristics. (rupress.org)
  • However, the combination of the bacterial part and the carrier protein induces a powerful and comprehensive immune response. (medicaltrend.org)
  • Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. (cshlpress.com)
  • The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)
  • E1/E3 deleted adenoviral reporter vector, CMV promoter, expresses green fluorescent protein. (umich.edu)
  • The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can't be integrated into host genome, guaranteeing the security for subsequent operations. (9lb.cc)
  • In addition, non-replicating mRNAs can be used to produce RNA-related vaccines that involve mRNA sequences encoding pathogen-specific proteins (antigens) in host cells. (medicaltrend.org)
  • When you construct a recombinant adenovirus genome in E. coli using cosmid technology, you are almost sure that every colony carries a full-size genome, and not a smaller plasmid that lost chuncks of the adenovirus genome by DNA recombination. (od260.com)
  • Both of them are flanking both ends of the recombinant adenovirus genome contained in your cosmid. (od260.com)
  • Because the PacI site is immediately flanking the start of the adenovirus genome, the DNA ends generated by PacI resemble the most the ends obtained from deproteinised virion DNA and might therefore be more efficient in promoting virus replication. (od260.com)
  • Based on a database search, we identified abemaciclib, an FDA-approved Cdk4/Cdk6 inhibitor used for the treatment of metastatic breast cancer, as potent inhibitor of HIPK2, HIPK3, and DYRK1A. (nature.com)
  • Among the most potent antagonists is non-structural protein 5 (NS5), which has been shown for all disease-causing flaviviruses to target different steps and players of the type I IFN signaling pathway. (shengsci.com)
  • Recombinant vaccinia virus and adenovirus have been developed as potent vaccine vehicles for treating cancer and infectious diseases. (dukecancerinstitute.org)
  • Significant E1A-dependent induction of c-fos mRNA and AP-1-binding activity was observed in cells expressing either E1A protein. (princeton.edu)
  • E1A-PLECs and E1A-NLFs positive for E1A DNA, mRNA and protein expressed cytokeratin and vimentin but not smooth muscle α-actin. (ersjournals.com)
  • In RNA vaccines, mRNA is used as a template for the endogenous synthesis of the target pathogenic protein (antigen). (medicaltrend.org)
  • Such vaccines do not include other protein sequences that induce self-amplification of the inserted mRNA sequence. (medicaltrend.org)
  • Some new promising strategies for biomarker discovery include microarray-based profiling at the DNA and mRNA levels, and mass-spectrometry-based profiling at the protein and peptide levels [ 2 ]. (biomedcentral.com)
  • Adenovirus is often used as a viral vector to produce vaccines with replication ability and vaccines with replication defects. (medicaltrend.org)
  • An E1/E3 deleted adenovirus type 5 vector expressing cre recombinase under CMV promoter control. (umich.edu)
  • this requires regions in E1a necessary for cellular transformation. (ox.ac.uk)
  • Adenovirus Protein VII Functions Throughout Early Phase and Interacts with Cellular Proteins SET and pp32 , 79 Journal of Virology 2474 (2005) (with Yuming Xue, David Ornelles, Judy Lieberman & Daniel Engel). (northwestern.edu)
  • Adv infects the host cells through binding to different cellular receptors such as coxsackievirus and adenovirus receptor (CAR) [ 12 ]. (hindawi.com)
  • During Adv DNA replication, host cellular proteins such as nuclear factor I and POU2F1 are used by Adv [ 13 , 14 ]. (hindawi.com)
  • When amplifying purified adenovirus that has been serially propagated it may be wise to isolate viral clones (with a plaque amplification), in order to avoid any possible problems with replication-competent adenovirus. (umich.edu)
  • Generally, pathogen products used in subunit vaccines include purified or recombinant protein-inactivated bacterial toxins, or polysaccharides present on surface capsules. (medicaltrend.org)
  • Moreover, the long-term systemic delivery of a recombinant protein is an expensive, painful experience for patients and is cumbersome for medical staff. (biomedcentral.com)
  • Recombinant protein expression of interest is detectable from 24 hours post infection. (9lb.cc)
  • It is postulated that this intermediate phenotype may play a major role in the remodelling of the airways in chronic obstructive pulmonary disease associated with persistence of adenovirus E1A DNA. (ersjournals.com)
  • Notably, intratumoral injection of this cBiTE-expressing adenovirus increased the persistence and accumulation of tumor-infiltrating T cells in vivo , compared with the parental virus lacking such effects. (aacrjournals.org)
  • E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)
  • These peptides were then tested in our previously described oncolytic cancer vaccine platform PeptiCRAd, a vaccine platform that combines an immunogenic oncolytic adenovirus (OAd) coated with tumor antigen peptides. (elifesciences.org)
  • Viral titers are determined by TCID50 method, which is the most accurate way to measure the titer of adenovirus. (9lb.cc)
  • Conjugate vaccines are made by combining polysaccharide residues in the bacterial surface capsule with carrier proteins (such as diphtheria toxoid protein). (medicaltrend.org)
  • Transduction results of various Adenovirus vectors. (9lb.cc)
  • pAd1129-01 is a shuttle plasmid designed for constructing E3-deleted and bipartite (dicistronic) recombinant adenovirus vectors. (od260.com)
  • Thus, miRNA-control of late proteins constitutes a novel strategy to provide selectivity to adenoviruses. (oncotarget.com)
  • Knowing the miRNA expression profile will help understand the role of miRNAs in modulating the host response to adenovirus infection and possibly improve the diagnosis of adenovirus-infected pneumonia. (hindawi.com)
  • The top 3 upregulated miRNA (hsa-miR-127-3p, hsa-miR-493-5p, and hsa-miR-409-3p) were identified in adenovirus-infected children and provided a clear distinction between infected and healthy individuals. (hindawi.com)
  • cdc2 protein kinase is complexed with both cyclin A and B: Evidence for proteolytic inactivation of MPF. (cshlpress.com)
  • enhancer contains two functionally distinct domains: one is specific for E1A and the other modulates all . (wikigenes.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Physical mapping of temperature-sensitive mutations of adenoviruses. (cshlpress.com)
  • Both exhibited cuboidal morphology and junctional complexes, but did not contain lamellar bodies or express surfactant protein A, B or C mRNAs. (ersjournals.com)
  • Adenovirus Type 5 DNA-Protein Complexes from Formaldehyde Cross-Linked Cells Early After Infection , 312 Virology 204 (2003) (with David Spector, Nicholas Baird & Daniel Engel). (northwestern.edu)
  • Several other genetic backgrounds result in enlargement of the haltere significantly beyond the normal range of haploinsufficient phenotypes, suggesting genetic variation in cofactors that mediate homeotic protein function. (lookformedical.com)
  • The 32-aa MYND domain (for myeloid, Nervy, and Deaf-1) contains non-DNA-binding zinc fingers that are thought to mediate protein-protein interactions (Gross, 1996). (sdbonline.org)
  • Together, our findings show that adenoviruses coding for CD40L mediate multiple antitumor effects including oncolysis, apoptosis, induction of T-cell responses, and upregulation of T(H)1 cytokines. (mek-inhibitors.com)
  • In this study, we corroborate that the SARS-CoV-2 non-structural proteins 3 and 4 (nsp3 and nsp4) suffice to remodel the endoplasmic reticulum to form double-membrane vesicles similar to ROs observed in viral infection. (bvsalud.org)
  • In this study, we corroborate that two viral proteins, nsp3 and nsp4, are the major drivers of membrane remodeling in SARS-CoV-2 infection. (bvsalud.org)
  • This chaperone increased in abundance on DENV infection via activation of the unfolded protein response (UPR). (bvsalud.org)
  • The present report concerns the role of adenovirus infection in inducing this transformation in an animal model of chronic obstructive pulmonary disease. (ersjournals.com)
  • These human observations are supported by experiments in guinea pigs, where, in the absence of actively replicating virus but the presence of E1A DNA, adenovirus infection resulted in excess lung inflammation following a single exposure to cigarette smoke 7 , and increased parenchymal and airway wall inflammation with greater emphysematous destruction after chronic exposure 8 . (ersjournals.com)
  • Systemic administration is necessary for the treatment of metastatic disease, however the intravenous administration of adenovirus results in viral retention in the liver causing significant infection of hepatocytes leading to liver damage [ 1 ]. (oncotarget.com)
  • Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children, which results in significant morbidity and mortality in children every year. (hindawi.com)
  • Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children [ 1 , 2 ]. (hindawi.com)
  • Here, we characterize such a protein, iASPP (inhibitory member of the ASPP family), encoded by PPP1R13L in humans and ape-1 in Caenorhabditis elegans. (ox.ac.uk)
  • E1a thus appeared to partially convert diverse human tumor cells into an epithelial phenotype. (rupress.org)
  • Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
  • Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
  • Furthermore, E1A DNA and protein persisted in epithelial cells in the lungs of these animals 9 . (ersjournals.com)
  • These highly upregulated microRNAs may have crucial roles in Adv pathogenesis and are potential biomarkers for adenovirus-infected pneumonia. (hindawi.com)
  • Expression of HAdV-5 CR3 in the yeast Saccharomyces cerevisiae inhibits growth, as do the Corresponding regions of the HAdV-3, 4, 9, 12 and 40 E1A Proteins, which represent the remaining five HAdV Subgroups. (birmingham.ac.uk)
  • In this study, we show that the wild-type Rb protein can specifically repress transcription from promoters driven by DRTF1 whereas a naturally occurring mutant Rb protein cannot. (ox.ac.uk)
  • Mouse S49 cells were infected with adenovirus variants expressing either the 243- or 289-amino acid E1A protein and treated with the cAMP analog dibutyryl-cAMP. (princeton.edu)
  • Western blot assay was used to determine the protein expression of nuclear factor kappa B (NF-κB) p65, p-NF-κB p65, IκBα, and p-IκBα in Huh-7 cells, which are the key targets of the NF-κB pathway. (bvsalud.org)
  • Moreover, KD-1 could significantly reduce the protein expression of p-NF-κB p65, NF-κB p65, and p-IκBα, while increasing the expression of IκBα in Huh-7 cells. (bvsalud.org)
  • An important pathogenicity factor is non-structural protein 1 (NS1) required for viral replication and secreted from infected cells. (bvsalud.org)
  • The transgenic expression of Ad5 E1A and E1B19K simulated the rescue effect of dl 1520 supporting Ad-Endo replication in GC cells. (biomedcentral.com)
  • Some of the new approaches depend on tumor biology and aim specifically to inhibit tumor growth and metastasis by targeting the tumor microenvironment or vasculature (leaving normal cells unaffected) or focusing on specific protein or signal transduction pathways. (medscape.com)
  • Shen, Y.H., Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice. (riken.jp)
  • Hence, an inhibitory form of ASPP resembling 53BP2 could allow cells to bypass the tumor-suppressor functions of p53 and the ASPP proteins. (ox.ac.uk)
  • Moreover, iASPP is an oncoprotein that cooperates with Ras, E1A and E7, but not mutant p53, to transform cells in vitro. (ox.ac.uk)
  • These messages direct cells to make the proteins that play many critical roles in the body. (ucla.edu)
  • Despite the existence of a highly immunosuppressive tumor environment, adenovirus-infected cells can nonetheless be efficiently cleared by infiltrating cytotoxic T lymphocytes (CTL) without compromising tumor burden. (aacrjournals.org)
  • Adenovirus has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues. (9lb.cc)
  • Selected results of Adenovirus transduction in the adult rat cardiac cells (A), eyes (B), neuron cells (C). (9lb.cc)
  • Homeodomain-interacting protein kinases (HIPKs) belong to the CMGC kinase family and are closely related to dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs). (nature.com)
  • Homeodomain-interacting protein kinases (HIPKs) are an evolutionarily conserved kinase family comprising the members HIPK1, HIPK2, and HIPK3 in vertebrates, and, additionally, HIPK4 in mammals. (nature.com)
  • A chemical or drug that inhibits the mitogen-activated protein kinase kinase enzymes MEK1 and/or MEK2. (mek-inhibitors.com)
  • Electrophoretic mobility shift assays were performed in order to examine nuclear factor (NF)-κB and activator protein (AP)-1 binding activities. (ersjournals.com)
  • The microvessel density and Ad replication in tumor tissue were evaluated by checking the expression of CD34 and hexon proteins, respectively. (biomedcentral.com)
  • Regulation of poly(A) site selection in adenovirus. (cornell.edu)
  • The adenovirus sequences are flanked by two SfiI sites, which generate non-symmetrical sticky ends suitable for directional cloning with the other AdenoQuick2.0 plasmids (pAd1127, pAd1128, pAd1130, and their derivatives). (od260.com)
  • We further report a number of host cell factors interacting with these viral proteins and supporting the viral replication cycle, some of them by contributing to the formation of the SARS-CoV-2 replication organelle. (bvsalud.org)
  • Both proteins contain a bromodomain flanking the HAT catalytic domain that is important for the targeting of CBP/P300 to chromatin and which offeres an opportunity for the development of protein-protein interaction inhibitors. (ox.ac.uk)
  • Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. (lookformedical.com)
  • by the coinjection of SV40 T- antigen, adenovirus E1A, or a high level of E 2 F - 1 expression plasmids. (wikigenes.org)
  • Expression profiles of whole blood microRNAs were altered and distinctly different in adenovirus-infected children. (hindawi.com)
  • Antennapedia homeodomain protein is a homeobox protein involved in limb patterning in ARTHROPODS. (lookformedical.com)
  • also known as ASH1), neurogenic differentiation factor 1 (NeuroD1), yes-associated protein 1 (YAP1) and POU class 2 homeobox 3 (POU2F3). (cancerindex.org)
  • BACKGROUND The histone methyltransferase (HMT) family includes histone lysine methyltransferases (HKMTs) and histone/protein arginine methyltransferases (PRMTs). (cancerindex.org)
  • A 1.9 kb XbaI fragment encoding gp19K membrane protein and the adenovirus "death" protein (ADP, 11.6K) was deleted, and replaced with a multiple cloning site. (od260.com)