Scratch wound healing assay. Medical search. Frequent questions

Cell migration was detected by wound healing and Transwell assays, while cell invasion was detected by a Matrigel‑coated Transwell method. (spandidos-publications.com)

PTEN was validated by dual luciferase assay as a target gene of miR-183. (nature.com)
The 3′UTR-binding sequence site of miR-128-5p on the p21 gene was predicted and assessed by luciferase assays. (hindawi.com)
Using bioinformatics, a luciferase reporter assay and western blot analyses, the current study identified that runt‑related transcription factor 2 (RUNX2) was a target of miR‑205 in PC and overexpression of miR‑205 suppressed the expression of RUNX2. (spandidos-publications.com)
A dual luciferase reporter assay was used to identify whether TGF-β is the target gene of miR-296-5p. (biomedcentral.com)
Dual luciferase reporter gene assay showed that miR-146a-5p can directly target FLAP mRNA. (biomedcentral.com)

HUVECs were administrated with TMZ or TMZ and lentivirus, the expression of EndMT and related proteins was observed by wound healing assay, immunoblotting, and immunofluorescence. (dovepress.com)
Then, immunofluorescence imaging, scratch wound-healing, transwell migration and invasion assays were used to observe the effects of miR-296-5p on cell metastasis and invasion. (biomedcentral.com)
Changes to the expression and phosphorylation of PAK1 and its effectors were determined by western blotting, and changes to the actin cytoskeleton were determined by performing an immunofluorescence assay. (biomedcentral.com)
A) Representative photos of FAK in C1632-treated and untreated A549R cells in immunofluorescence assays. (emlinhibitor.com)

The migration of these cells also increased as demonstrated by wound healing and transwell assays. (jcancer.org)
Our in vitro studies included scratch wound-healing migration assays and transwell assays to examine PPI's effect on HCC cell migration and invasion. (bvsalud.org)

The purpose of this scratch is to produce a cell-free area in hopes of inducing cells to migrate and close the gap. (wikipedia.org)
The scratch test is only ideal for cell types that migrate as a collective epithelial sheets and not useful for non-adherent cells. (wikipedia.org)
Also, the damage could physically damage the cells adjacent to the wound and create inaccurate wound size areas. (wikipedia.org)
The Electric Cell Impendance Sensing assays utilize to prevent damage to the cells in the underlying extracellular matrix that can likely happen with the manual scratching approaches. (wikipedia.org)
Additionally, there is still mention with the problem of accumulation of cells at the edge of the scratch, making the cell densities uneven. (wikipedia.org)
Outlined is a standard approach to carry-out this assay without the advanced technology: Plate cells of choice in growth medium into a live cell imaging dish or chamber slide. (wikipedia.org)
When the confluency of the cells are ideal, use a pipette tip to scratch a wound through the entire center of the well. (wikipedia.org)
The CytoSelect™ wound healing assay kits assess the migratory, proliferation and wound healing characteristics of cells. (bioscience.co.uk)
Overcoming the weaknesses of the traditional scratch method by using proprietary treated inserts that can generate a defined wound field or gap without impeding the proliferation or migration of cells, the CytoSelect™ wound healing assay kit produces accurate and consistent results. (bioscience.co.uk)
STO cells were tested using the CytoSelect™ 24-well wound healing assay. (bioscience.co.uk)
Cells were cultured 24h until a monolayer formed, at which time the inserts were removed to begin the assay. (bioscience.co.uk)
Proprietary inserts generate a consistent 0.9mm wound gap between the cells. (2bscientific.com)
Cells may then be treated or monitored for proliferation or migration across the wound field by imaging samples at fixed time points or by time-lapse microscopy. (2bscientific.com)
Treated with PGE2 and EP3 antagonist, RL95-2 cells were investigated by MTT, BrdU, and wound healing assay for functional assessment of EP3. (oncotarget.com)
The expressions of miR-296-5p in NPC tissues and cells were determined using GSE32920 database analysis and real-time PCR and miRNA microarray assays. (biomedcentral.com)
Researchers then conducted a cell viability assay and calculated the doubling time of the cells. (dermatologytimes.com)
Afterwards, they performed a scratch wound healing assay on NHDF cells by making a scratch in the cellular monolayer at confluence. (dermatologytimes.com)
Through significant differences in FPCL contraction rates between control and serum NHDF cells, as well as stronger growth states in cells treated with bovine sialoglycoproteins, researchers noted the capability of bovine sialoglycoproteins to aid in skin wound healing. (dermatologytimes.com)
In addition, the hemocompatibility assay revealed that the NPs do not significantly rupture red blood cells up to a dose of 1000 µg/mL. (biomedcentral.com)
Furthermore, in vitro scratch assay revealed that ZnFe 2 O 4 NPs improved cell migration and proliferation of cells, with noticeable shrinkage of the artificial wound model. (biomedcentral.com)
Therefore, for the management of burn wound infection, new antimicrobial agents that are effective against antibiotic-resistant bacteria, non-toxic to normal cells, cost-effective, and do not cause bacterial resistance are needed. (biomedcentral.com)
Wound Healing Assay Time series DIC images of mouse DA3 cells, derived from the mouse mammary adenocarcinoma cell line D1-DMBA-3, induced in BALB/C mice by dimethylbenzanthracene were grown to 90% confluence. (ucsd.edu)
A scratch was generated using a 200 µl tip, and the cells were incubated and subjected to time lapse confocal laser scanning microscopy (CLSM-510, Zeiss, Germany) for approximately 26 hrs, with images taken at 14.5 min intervals. (ucsd.edu)
On top of that, an Edu staining assay and flow cytometry have been performed to additional investigate regardless of whether C1632 inhibited the colony formation of A549 and/or A549R cells by DNA replication inhibitionand cell cycle arrest. (emlinhibitor.com)
C) C1632 inhibits migration of A549R cells inside the scratch-wound healing assay. (emlinhibitor.com)
D) Quantification of the results in (C). (E) C1632 inhibits migration and invasion of A549R cells in the Transwell assay. (emlinhibitor.com)

In vitro assays confirmed that the two compounds, to varying extents, inhibited RA-FLS proliferation, migration, invasion, and NF-κB signaling. (frontiersin.org)
The material from each step isscreened using acellular and in vitro assays for evaluating general toxicity, mechanisms of toxicity, and macrophage function. (cdc.gov)

The mechanism of cell cycle progression, cellular proliferation and caspase cascade activation was screened using scratch assay, flow cytometry, and mRNA expression analysis. (bvsalud.org)

HCT116 cell proliferation was assessed by conducting crystal violet and BrdU assays. (biomedcentral.com)

This is also called a scratch assay because it is done by making a scratch on a cell monolayer and capturing images at regular intervals by time lapse microscope. (wikipedia.org)
A scratch approximately 300um wide was made in the cell monolayer, and images were recorded every 14.5 min for 26 hr. (ucsd.edu)
Endothelial toxicity was evaluated by quantifying pro-inflammatory mRNA expression, monolayer permeability, and wound closure. (cdc.gov)
Conditioned medium from CBox-treated macrophages induced significant elevation in endothelial cell pro-inflammatory mRNA expression, enhanced monolayer permeability and impairment of scratch healing in CXCR3 dependent manner. (cdc.gov)

Our CytoSelect™ 24-Well Wound Healing Assay provides a much more consistent method to measure cell migration across a wound field gap in vitro. (2bscientific.com)

Damage to the skin such as wounding results in the loss of protection to the host and can permit the invasion of opportunistic pathogens, which can cause disruption to the healing process resulting in a chronic wound. (hud.ac.uk)
the cell migration and invasion were detected by Transwell assay. (cnki.net)
The results of Scratched wound healing assay and Tranwell assay showed that the overexpression of ECRG4 inhibited the cell migration and invasion. (cnki.net)

Additionally, the Woundmaker makes fast and uniform wounds across various numbered well-plates options (96 or 384) and allows for high throughput screening, which is a major advantage for various medical research studies. (wikipedia.org)

Moreover, down-regulation of MMP2/MMP9 scratch and matrigel assays revealed anti-metastatic properties of AgNPs. (springeropen.com)

In one study, 1 researchers sought to investigate the effects of sialoglycoproteins on normal human dermal fibroblasts (NHDF), citing the role of fibroblasts in contributing to aging and potential for chronic wounds, particularly in the elderly. (dermatologytimes.com)
Pseudomonas aeruginosa is an opportunistic pathogen and a coloniser of chronic wounds and is becoming increasingly recognised as major cause of hospital acquired infections due to its ability to secrete a variety of virulence and pathogenic factors such as toxins, proteases, vesicles and in addition biofilm formation. (hud.ac.uk)

The secretions from cultures grown with ethanol for 80 hours produced increased toxicity resulting in greater keratinocyte death and longer healing times in a scratch assay model of wound healing, in addition there was a high pro-inflammatory response from the keratinocytes compared to those exposed to live bacteria grown in the same conditions and also when compared to secretions from glucose grown PS3. (hud.ac.uk)
Toxicity and functional assays were also performed on murine macrophage (RAW 264.7) and endothelial cell lines (C166) with and without pharmacological inhibitors. (cdc.gov)

Using microscopic viewing and image software, researchers measured the scratch area and calculated wound closure. (dermatologytimes.com)

To assess the effects of EOPK on cell migration, we performed a wound-healing assay. (biomedcentral.com)

Despite the new technology that is increasing this assay's accuracy and efficacy, there are still confounding factors that can skew the assay results, such as cell "crowding", cell/cell adhesion effects and matrix effects. (wikipedia.org)
These results suggested that bovine sialoglycoproteins may be a candidate for anti-skin aging and scarless wound healing. (dermatologytimes.com)

Softer PSLipos-L-NAC could resist macrophage capture, but remarkably prolong their targeting effect period on macrophages via durable binding to macrophage surface, and subsequently more effectively suppress inflammatory response in macrophages and then hasten inflammatory lung epithelial cell wound healing. (biomedcentral.com)
Our previous work showed that lower Young's modulus (E) of PS-containing deformable nano-liposomes would greatly affect PS-receptor-mediated macrophage responses, including cellular internalization, anti-inflammatory and pro-healing cytokine production, but the synergistic effects of drug-loaded PS-containing deformable nano-liposomes on macrophage-based therapy for inflammatory injuries remain much less understood [ 13 ]. (biomedcentral.com)

Many different cellular assay kits to choose from. (ahdiagnostics.dk)

In PrestoBlue and live/dead assays, ZnFe 2 O 4 NPs exhibited dose-dependent cytotoxic effects on human dermal fibroblasts. (biomedcentral.com)

Due to its antimicrobial and wound healing-promoting activity, metal nanoparticles have attracted attention for dermatological applications. (biomedcentral.com)
This study indicated that ZnFe 2 O 4 NPs have the potential to be used as a future antimicrobial and wound healing drug. (biomedcentral.com)

The resistance of burn wound-related pathogens such as Staphylococcus aureus ( S. aureus ) and Pseudomonas aeruginosa ( P. aeruginosa ) to local and systemic anti-infective agents is making treatment more difficult [ 7 ]. (biomedcentral.com)

Its medical importance is its propensity to cause devastating central nervous system (CNS) infection after contact of infected saliva with mucosal fluids, wounds, or tissues. (medscape.com)

To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay. (nature.com)
PF cultures grown in the same conditions produced no measurable virulence factors and the secretions had no toxic effects on the keratinocytes, however faster healing in the scratch assay occurred for some conditions. (hud.ac.uk)
Investigation the biological effects of DC-SIGNR were verified by MTT, wounding and transwell in vitro and experiments on animals to confirm gastric cancer liver metastasis by IVIS. (biomedcentral.com)

Then monitor cell migration/wound healing in real-time. (ahdiagnostics.dk)
They also calculated the contraction rate of fibroblast-populated collagen lattice (FPCL) and conducted a reverse transcription-quantitative real time PCR, enzyme-linked immunosorbent assay, lectin histochemistry assay, SDS-PAGE and lectin blotting, western blot assay, and a statistical analysis. (dermatologytimes.com)

Burn wounds are the most lethal form of trauma that requires infection control to reduce morbidity and mortality [ 4 ]. (biomedcentral.com)
Primates with B virus infection are often asymptomatic, but B virus, in rare cases, can produce life-threatening disease in humans with mucosal or percutaneous contact (bite, scratch, splash, or needlestick) to primate saliva or other body fluids. (medscape.com)

A scratch wound-healing assay was used to detect the migration of VSMCs, and immunocytochemistry and western blotting for SM22ɑ were used to evaluate phenotypic transformation. (hindawi.com)

We also have RQSA - robust, quantitative scratch assay analysis system. (toronto.edu)
Combined with in-vitro proliferation assays using CRISPR-Cas9/RNAi and patient survival analysis, MEK inhibitors PD19830 and BRD-K12244279, pilocarpine, and tremorine were discovered as potential new drug options for treating breast cancer. (cdc.gov)

In this study, poly(ε-caprolactone) (PCL)/gelatin (GEL) electrospun nanofibers loaded with two different concentrations of Pinus radiata bark extracts (PEs) were fabricated via electrospinning for wound healing applications. (mdpi.com)
A wound healing assay is a laboratory technique used to study cell migration and cell-cell interaction. (wikipedia.org)
Traditionally scratch assays have been used to study cell migration, cell proliferation and wound healing. (2bscientific.com)
This study is designed to investigate the scope and bactericidal potential of zinc ferrite nanoparticles (ZnFe 2 O 4 NPs), and the mechanism of anti-bacterial action along with cytocompatibility, hemocompatibility, and wound healing properties. (biomedcentral.com)

Two strains of Pseudomonas (aeruginosa, hospital strain (PS3) isolated from a chronic wound dressing and (fluorescens, a laboratory reference strain (PF) that had no known virulence factors were grown in different media, supplemented with ethanol or glucose and in an additional media, simulated wound fluid for 24 or 80 hours. (hud.ac.uk)

This scratch can be made through various approaches, such as mechanical, thermal, or chemical damage. (wikipedia.org)

Bovine sialoglycoproteins may be effective agents in several dermatologic indications, including skin aging, scarring, and wounds. (dermatologytimes.com)
Wang X, Yue L, Zhang F, Bao X, Song W, Li Z. Roles of bovine sialoglycoproteins for anti‐skin aging and accelerating skin wound healing. (dermatologytimes.com)

One limitation of this assay is that there could be inconsistencies with the depth and size of the scratch. (wikipedia.org)

As mentioned previously, this is where the potential inconsistency comes into play with this assay. (wikipedia.org)

There are some skeptics who think that the scratch created for the assay isn't a very accurate representation of an actual wound. (wikipedia.org)

Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing. (nih.gov)

If the scratch is made manually, it is important to make sure that the wound is visible on both side of the field of view and should be around 0.5 mm wide. (wikipedia.org)

Specifically, this assay isn't ideal for chemotaxis studies. (wikipedia.org)

This is very likely true as real wounds are inherently more complex, but this assay does allow for collective cell movements under defined experimental conditions to provide some insight. (wikipedia.org)

As shown in Fig. 1D, chlorotyrosine substantially promoted cell migration during the wound healing process compared with the control. (bso14.org)

Confluent AoSMC were wounded by a scratch injury line made with a sterile cell scraper. (bso14.org)

The scratch tool for would healing lets you simultaneously produce 96 identical scratch wounds. (ahdiagnostics.dk)

In vitro kinase assay is applied to identify the kinase of PIK3CA/p110α tyrosine phosphorylation. (biomedcentral.com)

Anatomy13

Organisms8

Diseases22

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment13

Psychiatry and Psychology3

Phenomena and Processes14

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture4

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Detected by wound healing and Transwel1

Luciferase5

Immunofluorescence4

Transwell assays2

Cells26

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Flow cytometry1

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CytoSelect1

Invasion3

Uniform wounds1

Matrigel1

Chronic wounds2

Toxicity2

Closure1

Assess1

Results2

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