They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
Poor in vitro embryo development is a major obstacle in porcine assisted reproduction. (mdpi.com)
The application of reproductive biotechnology tools such as estrus synchronization (ES), artificial insemination (AI), embryo transfer (ET) through multiple ovulation and in vivo collection of embryos, in-vitro embryo production (IVEP) and cryopreservation, somatic cell nuclear transfer (SCNT) to produce the desired breed and sex of animal, and other reproductive biotechnologies that enhances reproduction potential are important innovations that can address specific needs and purpose for animal reproduction. (ifory.id)
This study aims to determine the ability of in vitro embryo cleavage of Bali cattle 48 hours after the fertilization process based on different ovarian reproductive status. (ifory.id)
Somatic4
Induced totipotent cells can be obtained by reprogramming somatic cells with somatic-cell nuclear transfer (SCNT). (wikipedia.org)
The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
Through somatic cell nuclear transfer, we finally obtain transgenic cattle with increased resistance to tuberculosis. (biomedcentral.com)
Somatic Cell Nuclear Transfer (SCNT-Cloning) is a promising technique in many areas and is based on genetically identical individuals. (pvb.com.br)
Vivo1
They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into endodermal, ectodermal and mesodermal cells both in vitro and in vivo. (wikipedia.org)
Membrane3
In the current study, we found that lamin A is mainly concentrated in the nuclear membrane. (cambridge.org)
The prelamin A is increased in both the nuclear membrane and nucleus, while phosphorylated lamin A (p-lamin A) is significantly reduced. (cambridge.org)
Finally, we propose the hypothesis that ZP3 can stabilize farnesylated prelamin A on the nuclear membrane of AIPL1, and promote its further processing into mature lamin A, therefore promoting the occurrence of GVBD. (cambridge.org)
Nucleus1
When ZP3 is knocked down, lamin A will be partially transferred to the nucleus of oocytes. (cambridge.org)
Fertilization3
After maturation, in vitro fertilization was carried out using TALP media with a final concentration of spermatozoa 1.5 x 106 spermatozoa/ml. (ifory.id)
Oocytes 5-6 after fertilization were washed 3 times using CR1aa media then cultured in 5% CO2 incubator, temperature 38.5 °C for 48 hours. (ifory.id)
SECM technique may be a valuable tool for accurately assessing the quality of embryos and thereby contribute to improving outcomes associated with assisted reproduction, including human in vitro fertilization. (bioone.org)
Supplement2
In the current study, we utilized modified Spirulina maxima pectin nanoparticles as a supplement to improve porcine in vitro maturation medium. (mdpi.com)
This study may be an important supplement for the mechanism of oocyte meiotic resumption and provide new diagnostic targets and treatment clues for infertility patients with oocyte maturation disorder. (cambridge.org)
Mechanism1
Little is known about the mechanism regulating the activation of primordial oocytes. (go.jp)
Involves1
A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Human1
hESCs can be generated by SCNT using dermal fibroblasts nuclei from both a middle-aged 35-year-old male and an elderly, 75-year-old male, suggesting that age-associated changes are not necessarily an impediment to SCNT-based nuclear reprogramming of human cells. (wikipedia.org)
Found2
In our previous studies, we found that zona pellucida 3 (ZP3) was strongly concentrated in the nuclear region of germinal vesicle (GV) oocytes and interacted with aryl hydrocarbon receptor-interacting protein-like 1 (AIPL1) and lamin A to promote GVBD. (cambridge.org)
It was found that FIGNL1, a member of MTSPs, was predominantly expressed in mouse oocytes and distributed at the spindle poles during meiosis in the present study. (cambridge.org)
Cells3
One example is the transformation of iris cells to lens cells in the process of maturation and transformation of retinal pigment epithelium cells into the neural retina during regeneration in adult newt eyes. (wikipedia.org)
The fact that transdetermination (change of the path of differentiation) often occurs for a group of cells rather than single cells shows that it is induced rather than part of maturation. (wikipedia.org)
After that, oocytes are collected by slicing and only oocytes with a cytoplasmic are homongent and have compact cumulus cells used in the study. (ifory.id)
Somatic cell nuc3
Developing of an optimized procedure for oocyte collection would be critical for allowing the use of in vitro embryo production technologies (IVEP) and somatic cell nuclear transfer (SCNT) as tools for rebuilding balance in animal numbers for such endangered species. (imv-technologies.com)
Induced totipotent cells can be obtained by reprogramming somatic cells with somatic-cell nuclear transfer (SCNT). (wikipedia.org)
In conclusion, a caffeine treatment at 1.25 mM during delayed activation for 4 h can improve the preimplantation development of porcine somatic cell nuclear transfer embryos by activating nuclear reprogramming. (koreamed.org)
Recipient oocyte3
The key important factors that have been identified for increasing the efficiency of transgenic animal production by SCNT are the quality of the recipient oocyte and the efficiency of nuclear reprogramming of the donor cell in the recipient oocyte [ 19 20 21 22 ]. (koreamed.org)
A high level of MPF activity affects nuclear envelope breakdown (NEBD) and premature chromosome condensation (PCC) of the donor cell nucleus in the cytoplasm of the recipient oocyte [ 24 25 ]. (koreamed.org)
The oocyte has two mtDNA haploplasmy states - mitochondrial heteroplasmy in the donor, and mitochondrial homoplasmy in the recipient oocyte. (fertiligy.com)
Zebrafish2
They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
Actin networks are responsible for driving ooplasm segregation in zebrafish oocytes. (fertiligy.com)
Donor5
90% of the number of follicles aspirated and an average of 32.6 oocytes/donor. (imv-technologies.com)
These data demonstrate that both donor age and grafting period may be important factors regulating the maturation of bovine testis xenografts, indicating that intrinsic differences exist within testis tissue at these donor ages. (bioone.org)
Therefore, efficient nuclear reprogramming of the donor cell in the oocyte determines the ultimate production efficiency of producing transgenic animals. (koreamed.org)
Of the many factors related to nuclear reprogramming of donor nuclei, it was reported that the maturation promoting factor (MPF) activity is important [ 23 ]. (koreamed.org)
Ooplasm donation is a technique where donor oocytes are used to make embryos. (fertiligy.com)
Nuclei1
hESCs can be generated by SCNT using dermal fibroblasts nuclei from both a middle-aged 35-year-old male and an elderly, 75-year-old male, suggesting that age-associated changes are not necessarily an impediment to SCNT-based nuclear reprogramming of human cells. (wikipedia.org)
Embryos3
Traditional transgenic techniques rely upon injection into one-cell embryos, but ideally these transgenes would be expressed only in adult somatic tissues. (bvsalud.org)
This study examined the effects of a caffeine treatment to improve nuclear reprogramming in porcine cloned embryos. (koreamed.org)
It also discusses the effects of ooplasmic transfer on oocytes and embryos, and mtDNA. (fertiligy.com)
Transgenic1
Porcine SCNT has been performed to produce transgenic pigs for use in xenotransplantation, and many transgenic pigs have been produced over the past decade [ 9 10 11 ]. (koreamed.org)
Process4
This transition can be a part of the normal maturation process, or caused by an inducement. (wikipedia.org)
One example is the transformation of iris cells to lens cells in the process of maturation and transformation of retinal pigment epithelium cells into the neural retina during regeneration in adult newt eyes. (wikipedia.org)
The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
The cytoplasmic streaming process in Drosophila oocytes offers a striking example of hydrodynamics in action. (fertiligy.com)
Quality1
Without ooplasmic regulators, oocytes can be rendered of low quality. (fertiligy.com)
Production1
Bovine ectopic testis tissue grafting is a technique that can be used to study bovine spermatogenesis and for the production of germ cells for a variety of applications. (bioone.org)