High affinityAntibodyMissing an affinity groupProteinsLigandAntibodiesReceptorReagentsMass SpectrometryPeptidePropagation2023PredictionResinPurificationScholarshipReceptorsFacultyGroup2017Biological SciencesFossilReductionDepartmentGroupsJournalPeriodsTransactionsMinimalUniversityInnovationCellMethodsKnowledgeStudyActivityMembersTechnologyEngineeringProvideLifeHumanShowPartnership
High affinity10
- Study of the regulatory properties of glucokinase by site-directed mutagenesis: conversion of glucokinase to an enzyme with high affinity for glucose. (diabetesjournals.org)
- A molecular database of bound conformations of 16 structurally diverse agonists was established by alignment with a high- affinity template compound for the D1 receptor, 3-allyl-6-bromo-7,8- dihydroxy-1-phenyl-2,3,4,5-tetrahydro-1H-benzazepin. (elsevierpure.com)
- Several of the lead compounds closely resembled high-affinity training set compounds. (elsevierpure.com)
- Establish overexpression of high-affinity CD16 on NK cells as a potential combination therapy for improving ADCC activity of anti-tumor monoclonal antibodies. (maxcyte.com)
- Specifically, demonstrate efficient expression of high-affinity CD16 in NK cells with minimal effects on cell viability and phenotype using mRNA electroporation and assess the effects of CD16-158V expression on rituximab - mediated cytotoxic activity against B cell lymphoma cells. (maxcyte.com)
- Ex vivo expanded NK cells electroporated with mRNA encoding the high-affinity Fc receptor, CD16-158V, demonstrated a significant increase in CD16 surface expression (Figure 1A). (maxcyte.com)
- Ex vivo expanded NK cells were electroporated with mRNA (4 µg/10 6 NK cells) encoding the high-affinity, CD16-158V Fc receptor. (maxcyte.com)
- We show here that the reactivity of a high affinity, virus-neutralizing human antibody against the AD-2 epitope of cytomegalovirus gB can be modified by introducing other Vkappa sequences together with the original VH sequence. (lu.se)
- It was also evident that the VH/Vkappa pairing was not promiscuous, since antibody fragments selected by phage display retained light chain sequences very similar to the original hybridoma-derived light chain, proving that a high affinity interaction was very dependent on a co-operativity between both variable domains. (lu.se)
- These findings show that phage display technology might modify the binding properties of pre-existing, high affinity antibodies. (lu.se)
Antibody10
- For the affinity purification of non-antibody products, affinity ligands (link is external) are lacking. (avacta.com)
- Monoclonal antibodies have proven typically too fragile to withstand the elution and cleaning procedures, prove sensitive to protease attack and exhibit difficulties with discrete antibody affinities preventing target elution without damaging the product from some systems, such as in cell purification screens. (avacta.com)
- One strategy that has been used to isolate the increasing array of non-antibody biopharmaceuticals is to tag target proteins and capture based upon the affinity-tag. (avacta.com)
- B ) Immuno- and affinity-histochemistry of a polyclonal anti-VEGFR2 antibody and of representative Affimers B8 and A9. (elifesciences.org)
- Patients homozygous for a natural CD16 polymorphism, CD16-158V which displays higher IgG1 and IgG3 affinity, have improved clinical outcomes upon anti-tumor therapeutic antibody treatment. (maxcyte.com)
- Antibody-free and selective detection of okadaic acid using an affinity peptide-based indirect assay. (bvsalud.org)
- Antibody binding to bacterial surfaces plays a crucial role in immunity, and a key characteristic of this protein-protein interaction is the binding affinity. (lu.se)
- Determining the affinity of an antibody binding to its antigen is the first step in predicting the function in a physiological environment where other competing protein interactions may be present. (lu.se)
- Antibody-antigen affinity is often evaluated with isolated proteins. (lu.se)
- In this chapter, we present a flow cytometry-based assay to measure and calculate the cell surface binding affinity or avidity of any mono- or polyclonal antibody solution. (lu.se)
Missing an affinity group1
- Notice we're missing an affinity group? (csteachers.org)
Proteins4
- Protein tagging is often undesirable in itself- particularly for therapeutic proteins where the affinity-tag may induce immunogenicity. (avacta.com)
- A methylated DNA affinity precipitation method was implemented to assay binding of proteins to methylated DNA. (biomedcentral.com)
- Methylated DNA affinity capture by methyl-CpG binding proteins produces fractions highly enriched for methylated DNA, suitable for coupling to next generation sequencing technologies. (biomedcentral.com)
- Such assays can be used to study binding affinity and recruitment specificity of either recombinant or cellular proteins [ 27 , 28 ]. (biomedcentral.com)
Ligand2
- Protein A affinity purification is the accepted industry standard for purifying monoclonal antibodies, yet multiple problems (link is external) present with industry use of this affinity ligand. (avacta.com)
- Estimates of ligand-binding affinities supported by quantum mechanical methods. (lu.se)
Antibodies1
- Taken together with their high production costs we can see that antibodies are less than ideal ligands for affinity purification processes. (avacta.com)
Receptor3
- To elucidate the role of affinity in regulating these processes, a technique for measuring B cell receptor affinity maturation in GCs in situ was developed. (bham.ac.uk)
- Antigenicity and receptor affinity of SARS-CoV-2 BA.2.86 spike. (cdc.gov)
- each has varying affinity for each receptor. (medscape.com)
Reagents2
- The rapid development times of alternative affinity reagents in affinity purification can reduce project-development timelines limiting costs and offer an essential competitive advantage, for example in the production of biosimilars. (avacta.com)
- This potential for new biopharmaceuticals and drugs to be easily purified at lower cost, through the use of prokaryotic systems is a major factor in the increased interest in and uptake of alternative affinity reagents for affinity purification applications across both research and industrial fields. (avacta.com)
Mass Spectrometry1
- These studies have combined peptide affinity pull-downs and quantitative mass spectrometry enabled by SILAC ( S table I sotope L abeling by A mino Acids in C ell Culture) [ 32 ]. (biomedcentral.com)
Peptide2
- In this study, we developed an affinity peptide -based direct competition enzyme-linked immunosorbent assay (dc- ELISA ) for the detection of OA in real samples. (bvsalud.org)
- These results suggest that the affinity peptide -based dc- ELISA can be a promising tool for detecting OA in shellfish samples. (bvsalud.org)
Propagation8
- In statistics and data mining, affinity propagation (AP) is a clustering algorithm based on the concept of "message passing" between data points. (wikipedia.org)
- Unlike clustering algorithms such as k-means or k-medoids, affinity propagation does not require the number of clusters to be determined or estimated before running the algorithm. (wikipedia.org)
- Similar to k-medoids, affinity propagation finds "exemplars," members of the input set that are representative of clusters. (wikipedia.org)
- The inventors of affinity propagation showed it is better for certain computer vision and computational biology tasks, e.g. clustering of pictures of human faces and identifying regulated transcripts, than k-means, even when k-means was allowed many random restarts and initialized using PCA. (wikipedia.org)
- A study comparing affinity propagation and Markov clustering on protein interaction graph partitioning found Markov clustering to work better for that problem. (wikipedia.org)
- Another recent application was in economics, when the affinity propagation was used to find some temporal patterns in the output multipliers of the US economy between 1997 and 2017. (wikipedia.org)
- A Julia implementation of affinity propagation is contained in Julia Statistics's Clustering.jl package. (wikipedia.org)
- Non-metric affinity propagation for unsupervised image categorization. (wikipedia.org)
20232
Prediction2
- We will focus on quantitative binding affinity prediction in order to fully characterize the activity spectrum of a drug. (videolectures.net)
- The resulting CoMFA models yielded cross-validated R 2 (q 2 ) values (standard error of prediction)of 0.879 (1.471, with five principal components) and 0.834 (1.652, with five principal components) for D1 and D2 affinity, respectively. (elsevierpure.com)
Resin2
- However, reusing the protein A resins leads to fouling of the protein A affinity resin. (avacta.com)
- A methylated DNA affinity resin can be applied to probe for MBD activity in extracts. (biomedcentral.com)
Purification6
- Between 60 and 80% of the downstream processing costs for biopharmaceuticals are attributed to the affinity purification process, where the biomolecule is purified from the complex cell background in which it has been manufactured, and a large component of these costs are taken up by the cost of affinity resins. (avacta.com)
- The exorbitant costs of protein A affinity resins on an industrial scale mean that biomanufacturers typically use multiple cycles of affinity purification per batch of product, to allow for the use of smaller protein A affinity purification columns thus keeping cost down. (avacta.com)
- To limit these processes with the initiation of smart monitoring systems would require an increased understanding of the factors that influence protein A affinity purification performance loss, though this knowledge is not currently available. (avacta.com)
- Tag-free affinity purification simplifies process-development, by reducing the number of process steps and the time required, making it more cost-effective and scalable for large scale production. (avacta.com)
- Over the past five years interest has rapidly increased in the development of downstream technologies, such as affinity purification. (avacta.com)
- The need to engineer new affinity ligands for use in affinity purification protocols rather than depending upon natural affinity ligands is clear. (avacta.com)
Scholarship1
- This scholarship is made possible by the fund raising efforts of the Ottawa Affinity Newfoundland and Labrador group and is valued at a portion of the income on the investment according to Memorial University of Newfoundland's Awards Program Spending Policy. (mun.ca)
Receptors3
- Subsequently, the developed AFM technique was applied in interrogation of B cells and hybridomas - expressing receptors of varying affinity towards NP - and two GCs in tissue sections (Chapter 6). (bham.ac.uk)
- We have previously shown that using agonist affinity at recombinant receptors selectively expressed in clonal cells as the dependent variable in three-dimensional quantitative structure-activity relationship studies (3D- QSAR) presents a unique opportunity for accuracy and precision in measurement. (elsevierpure.com)
- The ln(1/K(L)) values for the low-affinity agonist binding conformation at recombinant D1 and D2 dopamine receptors stably expressed in C6 glioma cells were used as the target property for the CoMFA (comparative molecular field analysis) of the 16 aligned structures. (elsevierpure.com)
Faculty1
Group9
- Interested in joining an affinity group? (csteachers.org)
- Browse our affinity group directory to learn about existing groups! (csteachers.org)
- Start by joining the associated virtual community to get connected, join a meet-up at the next CSTA event, or reach out to the Affinity Group lead to learn more! (csteachers.org)
- Why should a WWU student consider joining an affinity group? (wwu.edu)
- UNC student Nathan Wetzel plays Dungeons and Dragons with the Gender and Sexuality Resource Center's (GSRC) Queer D&D affinity group. (unco.edu)
- Welcome to Bear in Mind, I sat down with Nathan Wetzel, who has been playing Dungeons and Dragons with the Gender and Sexuality Resource Center's Queer DND affinity group. (unco.edu)
- An affinity group is a just kind of like specific group for people with maybe just one specific identity that they want to like. (unco.edu)
- So the affinity group kind of narrows down the queer community as a whole into the little subcategories that exist for those people of those labels, identities or interests to come together and have an even more succinct and smaller group. (unco.edu)
- Calvapilosa , in possessing a radula , unequivocally shows that other fossils like Halkieria [an ancient slug-like creature with two shell 'hats' found in Greenland] and Orthrozanclus [a spiny critter found in Canada's Burgess Shale deposit] belong to the molluskan … group," Van Roy told Live Science in an email. (livescience.com)
20171
Biological Sciences1
Fossil2
- Jefferies, R. P. S. Some fossil chordates with echinoderm affinities. (nature.com)
- Comparisons with fossil and modern conifer pollen grains suggest an affinity with conifers. (whiterose.ac.uk)
Reduction1
- A small reduction in the affinity for the regulatory protein was observed with mutations of residues on the smaller domain and in the hinge region, confirming the bipartite nature of the binding site for the regulatory protein. (diabetesjournals.org)
Department2
- I describe them as an armored, spiny slug with one single shell at the head end," said the study's co-lead researcher, Luke Parry, a doctoral student in the Department of Earth Sciences at the University of Bristol in England. (livescience.com)
- Department of Food Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea. (bvsalud.org)
Groups8
- CSTA Affinity Groups provide a space for CSTA members to connect with other members who share a common identity or interest. (csteachers.org)
- CSTA Affinity Groups host events, connect year-round via the CSTA Virtual Community and provide opportunities for members to meet with one another at CSTA in person and virtual events. (csteachers.org)
- We review these applications on a rolling basis, and look forward to welcoming new affinity groups to help CSTA members connect! (csteachers.org)
- Affinity groups provide another way to find your community within CSTA. (csteachers.org)
- Affinity groups can include professional networking groups, caucuses or councils and provide forums for groups of students or employees who share a common background of interest. (wwu.edu)
- In this episode they talk about what the GSRC and affinity groups are. (unco.edu)
- Some content may only be accessible if you belong to specific Specialty or Affinity Groups. (aag.org)
- and it is evident, on glancing at the subject cursorily, that any classification that brings together in groups diseases that have considerable affinity, or that are liable to be confounded with each other, is likely to facilitate the deduction of general principles. (cdc.gov)
Journal1
- American Journal of Science , 245 , 337-365. (palass.org)
Periods1
- Many recent comparative analyses of hominin footprints have used 3D analytical methods to assess their morphological affinities, comparing tracks from different locations and/or time periods. (bournemouth.ac.uk)
Transactions1
- Transactions of the Royal Society of Edinburgh: Earth Sciences , 92 , 15-37. (palass.org)
Minimal1
- Two mutations increased the affinity for glucose by twofold (K296M) and sixfold (Y214A), the latter also decreasing the Hill coefficient from 1.75 to 1.2 with minimal change in the affinity for ATP. (diabetesjournals.org)
University5
- Applicants must have a minimum of 60 credits earned, enrolled at an accredited college or university in the state of Minnesota or an Affinity Plus member studying out-of-state at the time of award in the fall semester. (petersons.com)
- White Rose Research Online is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. (whiterose.ac.uk)
- 2 Plant Science Research Laboratory, Unité Mixte de Recherche 5546, Centre National de la Recherche Scientifique/University of Toulouse 3, 31320 Auzeville Tolosane, France. (nih.gov)
- With a variety of degree programs in the arts, humanities, sciences, engineering, and professional disciplines, the university is well known for its learn-by-doing approach and Teacher Scholar Model . (ashg.org)
- Heather Massey, PhD, a senior lecturer in Sport, Health and Exercise Science at University of Portsmouth blames her father, a dinghy sailor, for her affinity for cold-water swimming. (medscape.com)
Innovation1
- Engineered affinity ligands have the potential to isolate higher capacities of a wider variety of targets with the ability to calibrate the target affinity to the required application, allowing vital process innovation. (avacta.com)
Cell1
- B ) Western blot showing Affimer-mediated affinity-precipitation of endogenously expressed Grb2 protein from U2OS cell lysates using five Grb2 Affimers bound to colbalt magnetic beads (n = 2). (elifesciences.org)
Methods1
- Most of the methods require a cut-off threshold in order to classify a K-mer as a binding site (BS) and finding such a threshold is usually done by handcraft rather than a science. (biorxiv.org)
Knowledge1
- Materials Today is a community dedicated to the creation and sharing of materials science knowledge and experience. (materialstoday.com)
Study1
Activity1
- these changes in PIP 2 affinity have corresponding effects on channel activity. (aspetjournals.org)
Members1
- Established in partnership with the Affinity Plus Foundation, scholarships will be awarded to Minnesota students or Affinity Plus members studying out-of-state pursuing a career related to the music industry. (petersons.com)
Technology2
- Separation Science And Technology , 45 (15), 2187-2193. (unl.pt)
- With the further development of science and technology, tremendous opportunities and broad prospects for the development of EIs have emerged. (cdc.gov)
Engineering2
- Image: Biomaterials Science & Engineering. (materialstoday.com)
- By making accurate predictions of water repulsion and protein adsorption for even hypothetical materials, the researchers' approach, reported in a paper in ACS Biomaterials Science & Engineering , opens up new possibilities for the screening and design of organic materials with desired functions. (materialstoday.com)
Provide1
- 1. Provide affinity spaces. (harvard.edu)
Life1
- Kolata, D. R., Frest, T. J. & Mapes, R. H. The youngest carpoid: occurrence, affinities and life mode of a Pennsylvanian (Morrowan) mitrate from Oklahoma. (nature.com)
Human1
- To identify the amino acids involved in the specific regulatory properties of glucokinase, and particularly its low affinity for glucose, mutants of the human islet enzyme have been prepared, in which glucokinase-specific residues have been replaced. (diabetesjournals.org)
Show1
- All the metasediments show affinity for sedimentary parentage, the granite gneiss is probably of igneous origin while the intrusives are magmatic. (earthsciencesmalaysia.com)
Partnership1
- Lavalier Personal Jewelry Insurance and Gemological Science International (GSI) announced an affinity partnership today. (berkleyassetpro.com)