• First, we cocultured dermal fibroblasts with dermal microvascular endothelial cells at a ratio of 2 : 1. (hindawi.com)
  • A fibrous sheet was formed by the interactions between the fibroblasts and the endothelial cells, and capillary-like structures were observed after 20 days of coculture. (hindawi.com)
  • More recent skin substitutes combine epidermal and dermal layers by introducing fibroblasts and keratinocytes into an acellular matrix [ 5 ]. (hindawi.com)
  • Subsequently, the scaffold was bioconjugated with apelin-13 using the EDC-NHS linker and acellular scaffolds were recellularized using fibroblasts, endothelial cells, and smooth muscle cells. (biomedcentral.com)
  • FBN2 silencing recapitulates hypoxic conditions and induces elastic fiber impairment in human dermal fibroblasts. (mcgill.ca)
  • They include endothelial cells, fibroblasts, and keratinocytes. (frontiersin.org)
  • Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. (nature.com)
  • As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. (nature.com)
  • Generally, wound healing in the oral cavity is known to occur more quickly and scar less than dermal tissue, which may be due to the elements in saliva and unique phenotype of oral fibroblasts ( 14 - 16 ). (spandidos-publications.com)
  • This decellularization method has been used in a variety of contexts including primary human dermal and lung fibroblast cultures, cancer-associated fibroblast cultures from esophageal, ovarian, renal, pancreatic, prostatic and lung tumors, and murine mammary fibroblasts 10,12,13 . (researchsquare.com)
  • To this end, primary fibroblasts were seeded at a high density (25,000 cells per cm 2 ) and cultured for 7 days, as originally described for mouse NIH-3T3 cells, to generate 3D cultures 11 . (researchsquare.com)
  • In vitro experiments withcardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibilityof BC-Ppy composites. (ibecbarcelona.eu)
  • Stratagraft (allogeneic cultured keratinocytes and dermal fibroblasts in murine collagen-dsat) is an allogeneic cellularized scaffold product used for the treatment of adults with thermal burns containing intact dermal elements for which surgical intervention is clinically indicated (also known as deep partial-thickness burns). (drugbank.com)
  • Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
  • The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
  • PTHrP-2 intensified intercellular communication between endothelial cells and fibroblasts and initiated endothelial-epithelial intercellular communication. (biomedcentral.com)
  • Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
  • She was awarded with a postdoc fellow from the Ministry of Science at the Technical University of Catalonia in Biomaterials for bone tissue regeneration and since 2010 has been professor at this university. (ibecbarcelona.eu)
  • Advances in bioengineered tissue mimetics, including three-dimensional ovarian follicle culture, represent an important new avenue of investigation in the study of normal reproductive function and the regeneration of diseased tissues [ 5 ]. (biomedcentral.com)
  • By accelerating wound closure, enhancing re-epithelialization, increasing angiogenesis, promoting granulation tissue formation, modulating inflammation, and regulating ECM remodeling, administration of MSCs has demonstrated a beneficial effect on cutaneous wound healing and skin regeneration (Fig. 1 ). (biomedcentral.com)
  • The differences, physiologically speaking, between the repair and regeneration of a tissue are subtle. (barefacedtruth.com)
  • Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)
  • For many years there is an urgent need in the field of regenerative medicine to develop replacements of non-functional tissues. (nature.com)
  • Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)
  • Sustained hypoxia or lack of nutrients leads to fibroblast dysfunction, decreased keratinocyte migration and proliferation, and tissue loss [ 6 ]. (hindawi.com)
  • Furthermore, the results demonstrated considerable elevation of cell adhesion and proliferation in scaffolds bioconjugated with apelin-13. (biomedcentral.com)
  • Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
  • The remaining follicular cells develop into a corpus luteum that produces E and progesterone (P). In turn, P promotes more proliferation within the uterus and vagina, and cornification in the vagina. (biomedcentral.com)
  • In vitro direct effects of PTHrP-2 were determined by proliferation, migration, Vascular Endothelial Grown Factor and collagen I secretion of cells and Akt/ Erk1/2 pathway change. (biomedcentral.com)
  • In vitro, PTHrP-2 promoted proliferation and migration of endothelial and fibroblast cells, but had no effect on epithelial cells. (biomedcentral.com)
  • HA is reported to have a role in several cellular interactions (cell differentiation, proliferation, development, and recognition) and physiological processes (lubrication, hydration balance, matrix structure, and steric interactions). (biotechnolabs.com)
  • ELISA demonstrated that vascularization was promoted by significant upregulation of vascularization associated growth factors due to interactions among the 3 types of cells in the bilayer, as compared to cocultures of fibroblast and endothelial cells and monocultures. (hindawi.com)
  • Most of these methods involve culturing endothelial cells on or within ECM substrates (e.g., collagen, fibrin, fibronectin, laminin, etc.) or other types of biomaterials to form capillary-like structures [ 15 ]. (hindawi.com)
  • We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
  • During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
  • Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
  • Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)
  • We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. (nature.com)
  • The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. (nature.com)
  • The mRNA and protein expression of matrix metalloproteinase 2 (MMP2) and vascular endothelial growth factor (VEGF) in Tβ4-treated RP cells was assessed by quantitative polymerase chain reaction (RT-qPCR) and western blot analysis, respectively. (spandidos-publications.com)
  • In addition, extracellular, administered Tβ 4 promoted the cell migration of various types of cells including cardiomyocytes, human umbilical vein endothelial cells and conjunctival epithelial cells ( 4 - 6 ). (spandidos-publications.com)
  • 2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
  • Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. (lww.com)
  • 1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
  • Fibrocytes, fibers, endothelial cells and capillaries construct the corium layer, whereas epithelial cells compose the dermal layer. (biomedcentral.com)
  • Endothelial cells and epithelial cells attach within or on the scaffold and form capillaries and the dermal layer. (biomedcentral.com)
  • Previous studies by other scholars and our research group have shown that PTH can not only stimulate osteoblasts but also endothelial cells and promote angiogenesis [ 17 ]. (biomedcentral.com)
  • Endothelial cells, which form the inner lining of microvessels, create this semipermeable barrier via a combination of cellular junctions, their glycocalyx, and the basement membrane that consists of vessel-specific extracellular matrix (ECM) [ 2 ]. (biomedcentral.com)
  • Embedded within the basement membrane and in direct contact with endothelial cells are pericytes, which are crucial for microvessel maturation, maintenance, as well as proper function. (biomedcentral.com)
  • The microvascular system and especially endothelial cells play a major role in inflammation, act as an endocrine organ and tightly regulate blood coagulation [ 1 ]. (biomedcentral.com)
  • A static tensile test was applied using a strength testing device to assess the mechanical properties of the electrospun scaffolds. (bvsalud.org)
  • The myocardial nicheplays a critical role in directing the function and fate of cardiomyocytes;therefore, engineering a biomimetic scaffold holds excellent promise.We produced an electroconductive cardiac patch of bacterial nanocellulose(BC) with polypyrrole nanoparticles (Ppy NPs) to mimic the naturalmyocardial microenvironment. (ibecbarcelona.eu)
  • Epithelial cells were then seeded on the fibrous sheet to assemble the bilayered tissue. (hindawi.com)
  • Exosomes were co-cultured with original cells, HUVECs or HFF-1 cells, and epithelial cells. (biomedcentral.com)
  • Loss of tissue is then repaired in a complex process that involves tissue-resident immune, stromal, and epithelial cells, as well as infiltrating immune cells [ 2 ]. (biomedcentral.com)
  • The 3T3 cell line was developed in 1962 and is derived from mouse embryonic fibroblast cells. (wikipedia.org)
  • Upregulation of Tβ 4 by cDNA-mediated transfection was found to cause actin depolymerization in fibroblast cells ( 1 ), which demonstrated that the modulation of Tβ 4 affected the polymerization state of actin cytoskeleton, as well as other cell processes associated with the organization of actin cytoskeleton, such as cell migration. (spandidos-publications.com)
  • These findings reveal that the incubation of the scaffold with Bio-Beads SM-2 is a novel and promising approach for increasing cell viability and growth within the scaffold. (biomedcentral.com)
  • The 3T3 Neutral Red Uptake (NRU) Phototoxicity Test, approved by the OECD, detects the viability of 3T3 cells after exposure to a chemical in the presence or absence of light. (wikipedia.org)
  • We also developed a novel strategy to eliminate the remnant detergents from the scaffold and increase cell viability by incubating acellular scaffolds with Bio-Beads SM-2 resin. (biomedcentral.com)
  • In conclusion, our results provide a platform in which xenograft vessels are decellularized properly in a short time, and the recellularization process is significantly improved after the bioconjugation of the acellular scaffold with apelin-13 in terms of cell adhesion and viability within the scaffold. (biomedcentral.com)
  • Cell viability, adhesion and migration of primary cultured RP cells were observed in the presence of Tβ4 at various concentrations ranging from 1 to 1,000 ng/ml. (spandidos-publications.com)
  • Tβ4 did not affect cell viability and adhesion, but RP cell migration was stimulated by Tβ4 at concentrations of 100 and 1,000 ng/ml. (spandidos-publications.com)
  • BC-Ppy scaffolds improved cell viability andattachment, promoting a desirable cardiomyoblast morphology. (ibecbarcelona.eu)
  • Skin tissue substitutes must readily adhere, have good physical and mechanical properties, and be nonantigenic [ 2 ]. (hindawi.com)
  • 50 Moreover, the mechanical properties of engineered tissues can also be improved by culturing under mechanical stimulation. (researchgate.net)
  • 43 Therefore, cell sheets with enhanced thickness and mechanical properties obtained through appropriate cyclic mechanical stimulation possess great potential for bone tissue engineering applications. (researchgate.net)
  • Testometric tensile testing machine was also used for the assessment of mechanical properties and uniaxial tensile strength of decellularized and recellularized vessels compared to that of native tissues. (biomedcentral.com)
  • These CDMs have distinct mechanical properties such as thickness, structure, and density compared to tissue harvests usually requiring optimized reagents, reagent concentrations, and incubation times 9 . (researchsquare.com)
  • On the other hand, even if 3D aggregate culture methods are able to recapitulate in vitro the cell-extracellular matrix interactions properly observed in vivo, and the synthetic/natural matrix and scaffolds have biochemical and mechanical properties, in order to mimic the native extracellular matrix, both of these systems possesses some limitations and some methodological improvements are needed. (razavihospital.ir)
  • Thus, the long-term survival and function of 3D tissues depend on the rapid development of new blood vessels to provide nutrients and oxygen to cells in the center of the tissue grafts. (hindawi.com)
  • Moreover, the harvest of vessels prolongs the surgical procedure and it may lead to the morbidity of donor site in elder patients: therefore, it seems that the use of tissue-engineered vessels would be an attractive and less invasive substitute for autologous vascular grafts. (biomedcentral.com)
  • One way is to carry out the transplantation, but the success of this strategy is complicated by immune reactions to allogeneic or xenogeneic grafts or deficiency of donor tissue. (nature.com)
  • Autogenous gingival grafts are often accompanied by discomfort, pain and retarded tissue repair depending on a patient's condition. (spandidos-publications.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • Exogenous Tβ 4 was also reported to accelerate the tissue repair of damaged cardiac, corneal and dermal tissues ( 4 , 6 , 13 ), which demonstrates potential for clinical applications in wound healing. (spandidos-publications.com)
  • Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
  • Our results highlight the remarkablepotential use of BC-Ppy scaffolds as a cardiac patch in tissue regenerativetherapies. (ibecbarcelona.eu)
  • Nano-thin and nano-porous membranes and heart like electric stimulus were used in the cardiac-mimetic cell-culture system. (thno.org)
  • An immunologically identical cardiac tissue can be produced by direct reprogramming from the patients' tissue without the potential risk of tumorigenesis that occurs with induced pluripotent stem cells [ 2 ] and the ethical issue using embryonic stem cells. (thno.org)
  • Extracellular matrix scaffolds derived from porcine small intestinal submucosa (SIS-ECM) have been shown to promote the formation of site-specific tissue in a number of preclinical animal studies. (researchgate.net)
  • However, efficient harvest and handling of cell sheets remain challenging, including insufficient extracellular matrix content and poor mechanical strength. (researchgate.net)
  • Mechanical loading has been widely used to enhance extracellular matrix production in a variety of cell types. (researchgate.net)
  • We found that the extracellular matrix content and thickness of cell sheet were markedly elevated upon appropriate mechanical conditioning. (researchgate.net)
  • Our results proposed 16-h perfusion of 0.25% sodium dodecyl sulfate (SDS) + 0.5% Triton X-100 combination to the vessel as an optimal decellularization protocol in terms of cell elimination as well as extracellular matrix preservation. (biomedcentral.com)
  • The dynamics of cell-extracellular matrix interactions in normal and pathological situations. (mcgill.ca)
  • The Reinhardt laboratory is interested in components and mechanisms involved in the biogenesis, function and pathological aspects of the extracellular matrix and its interactions with cells. (mcgill.ca)
  • We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)
  • These CDMs can be used to study cell interactions with the extracellular matrix (ECM) by reseeding cells in them. (researchsquare.com)
  • 8 nm).Ppy NPs effectively augment the conductivity, surface roughness, andthickness of BC composites despite reducing scaffolds' transparency.BC-Ppy composites were flexible (up to 10 mM Ppy), maintained theirintricate 3D extracellular matrix-like mesh structure in all Ppy concentrationstested, and displayed electrical conductivities in the range of nativecardiac tissue. (ibecbarcelona.eu)
  • The research themes rank around molecular mechanisms matrix proteins and fibers exert in disorders affecting blood vessels, bone, skin and adipose tissue. (mcgill.ca)
  • 48,49 In addition, cyclic mechanical strain enhances the function and development of engineered tissues by improving the production of collagen and elastin. (researchgate.net)
  • Scaffold choice, immunogenicity, degradation rate, toxicity of degradation products, host inflammatory responses, fibrous tissue formation due to scaffold degradation, and mechanical mismatches with the surrounding tissue are key issues that may affect the long-term behavior of the engineered tissue constructs and directly interfere with their primary biological functions [ 17 ]. (hindawi.com)
  • Keloids are the result of an overgrowth of dense fibrous tissue that usually develops after healing of a skin injury. (medscape.com)
  • Excessive fibrous tissue is classified as either a keloid or a hypertrophic scar. (medscape.com)
  • Different types of scaffolds containing these factors have been combined to enhance capillary formation in dermal wound healing models [ 9 , 10 ]. (hindawi.com)
  • Advances in tissue engineering technology have led to the production of novel human skin equivalents and organoids that reproduce cell-cell interactions with tissue-scale tensional homeostasis, and enable us to evaluate skin tissue morphology, functionality, drug response and wound healing. (mdpi.com)
  • Enhancement of RP cell migration and angiogenesis is likely to be involved in the promotion of wound healing. (spandidos-publications.com)
  • Besides the effect on cell migration, exogenous Tβ 4 was found to have multifunctional activities such as angiogenesis, anti-apoptosis, anti-oxidative stress and anti-inflammation ( 8 - 11 ), which emphasize its therapeutic potential in the repair of damaged tissues or wound healing. (spandidos-publications.com)
  • Despite the relatively rapid wound healing, however, tissues damaged during periodontal and implant surgery are continuously challenged by bacterial infection in the oral cavity, necessitating meticulous maintenance of oral hygiene and additional plaque control. (spandidos-publications.com)
  • Therefore, an optimal approach might involve the target of all three cell types to promote wound healing via synergistic effects. (biomedcentral.com)
  • We have critically reviewed the results of the novel studies that show promise for the continued development of MSC-based wound-healing therapies and provide direction for continued research in this field. (biomedcentral.com)
  • Enhanced cutaneous wound healing by mesenchymal stem cells. (biomedcentral.com)
  • Collagen is involved in the wound-healing process, where it helps to form a scaffold for new tissue growth and repair. (intactone.com)
  • The subsequent wound healing processes aim to restore dermal and epidermal integrity and functionality. (biomedcentral.com)
  • In this context, regulatory T cells (Tregs) hold a key role in balancing immune homeostasis and mediating cutaneous wound healing. (biomedcentral.com)
  • The maintenance of tissue homeostasis and proper wound healing is, therefore, a critical process that can be disturbed by a plethora of external and internal factors. (biomedcentral.com)
  • While researchers agree on the abundance of Tregs in distinct dermal layers, the exact involvement of Tregs in cutaneous wound healing remains to be elucidated [ 18 ]. (biomedcentral.com)
  • Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
  • Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
  • The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. (nature.com)
  • Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs. (nature.com)
  • Results: The processes by which re-aggregated adult single cell types or adult and embryonic explanted tissues are able to recapitulate embryogenesis in vitro, when cultured in adhesion or embedded in 3D gels, is not surprising and is clearly under the control of a reminiscent cellular memory which recapitulates early developmental stages. (razavihospital.ir)
  • HA can bind to the three main classes of cell surface receptors, each having a unique role: CD44 (a membrane glycoprotein), RHAMM (the receptor for hyaluronate-mediated motility), and ICAM-1 (intercellular adhesion molecule 1).CD44 is the most widely distributed cell surface receptor recognised for HA binding Other ligands that interact with CD44 include osteopontin, collagens, and matrix metalloproteinases (MMPs). (biotechnolabs.com)
  • Using functional annotation, we found that proteins belonging to pathways associated with regulation of the actin cytoskeleton, EGF/EGFR, TGF-β, MAPK signaling, integrin-mediated cell adhesion, and lipid metabolism were significantly enriched in the OA samples (p ≤10 −5 ). (biomedcentral.com)
  • In addition, keloids and hypertrophic scars differ from healthy skin by a rich vasculature, high mesenchymal cell density, and thickened epidermal cell layer. (medscape.com)
  • These epidermal and connective tissue layers protect against mechanical and chemical harm and repel pathogenic invaders [ 19 ]. (biomedcentral.com)
  • Two major alternatives to in vivo animal testing are in vitro cell culture techniques and in silico computer simulation. (wikipedia.org)
  • The testing of cosmetic products directly onto an animal can be minimized or eliminated by the use of in vitro cell growth and development. (wikipedia.org)
  • Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)
  • Therefore, the implantation of human cells reprogrammed in vitro represents an alternative strategy [ 16 - 19 ]. (thno.org)
  • Moscona A. The Development in Vitro of Chimeric Aggregates of Dissociated Embryonic Chick and Mouse Cells. (razavihospital.ir)
  • Kurosawa H. Methods for inducing embryoid body formation: in vitro differentiation system of embryonic stem cells. (razavihospital.ir)
  • An in vitro investigation examined the interaction between HA and exogenous sulfate-labeled cartilage proteoglycans (PGs) at the calf articular cartilage chondrocyte cell surface. (biotechnolabs.com)
  • To determine the general biological conse quence of BRG1 re expression in SK MEL5 cells, we investigated if BRG1 promotes alterations in the ability of melanoma cells for being invasive in vitro. (histaminereceptor-signal.com)
  • b) Computer-controlled application of a vacuum uniaxially strains the membranes, driving the loading pins apart to stretch the growth channel, and apply controlled dynamic strain to developing scaffold-free tendon fibers, emulating tensile strains seen in tendon development in vivo [90]. (researchgate.net)
  • 2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
  • Through repair phenomenon in vivo some problems were detected, and further research on their mechanisms was made. (biomedcentral.com)
  • Although exact figures are difficult to obtain, some have estimated that one million foetal cows are sacrificed each year to obtain the world's supply of foetal bovine serum, used to grow cultured cells. (wikipedia.org)
  • Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
  • However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. (lww.com)
  • EpiDerm from Mattek and EpiSkin and SkinEthic RHE model are derived from human skin cells which have been cultured to produce a model of human skin. (wikipedia.org)
  • We showed that ZIKV interacts with the AXL receptor to infect astrocytes and microglial cells in human fetal brain (Meertens et al. (gencelldis.fr)
  • These IMRCs are differentiated from human embryonic stem cells that are expanded and then harvested. (drugbank.com)
  • Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
  • Recombinant human/mouse/rat BMP-2 (355-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. (novusbio.com)
  • Collagen is the most abundant protein in the human body and is a fundamental component of connective tissues. (intactone.com)
  • Shih CC, Forman SJ, Chu P, Slovak M. Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice. (razavihospital.ir)
  • Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel. (razavihospital.ir)
  • A method is provided for reducing the effects of psychologically-mediated stress on the skin of a human or animal which method comprises administering to the individual a composition capable of inhibiting glucocorticoid-induced chronic stress in a dermal cell pr a cell involved in skin inflammatory responses. (allindianpatents.com)
  • In a related aspect, the present invention provides the use of a composition capable of inhibiting glucocorticoid-induced chronic stress in a dermal cell or a cell involved in skin inflammatory responses in the manufacture of a composition for use in reducing the effects of psychologically-mediated stress on the skin of a human or animal. (allindianpatents.com)
  • Ito et al ( 2 ) showed that overexpression of Tβ 4 enhanced the formation of actin-based pseudopodia and cell motility in prostate cancer cells, providing direct evidence that Tβ 4 plays a role in cell migration. (spandidos-publications.com)
  • Colon cancer cells overexpressing Tβ 4 also exhibited enhanced cell migration, due in part to the activation of the Rac1 signaling pathway ( 3 ). (spandidos-publications.com)
  • The mechanism by which exogenous Tβ 4 influences cell migration remains to be elucidated. (spandidos-publications.com)
  • The models use isolated primary support cells cultured onto a biological scaffold and within a microfluidic system to create a niche and support the desired differentiation of epithelia, germ and somatic cells from patient-derived induced pluripotent stem cells. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) are characterized by the ability to self-renew and exhibit differentiation into multiple tissue-forming cell lineages, such as osteoblasts, adipocytes, chondrocytes, tenocytes, and myocytes [ 1 ]. (biomedcentral.com)
  • Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics. (razavihospital.ir)
  • Czyz J, Wobus A. Embryonic stem cell differentiation: the role of extracellular factors. (razavihospital.ir)
  • Engineering the embryoid body microenvironment to direct embryonic stem cell differentiation. (razavihospital.ir)
  • therefore, covalent attachment of apelin-13 to the acellular scaffolds might be a favorable approach for improving recellularization efficacy. (biomedcentral.com)
  • SEM images and characterization methods were also used to evaluate the effect of apelin-13 attachment to the acellular scaffold on tissue recellularization. (biomedcentral.com)
  • In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)
  • However, these cytokines are often expensive and have strict limitations regarding use currently used factors exert their healing abilities in either the dermal layer or corium layer of wounds [ 8 ]. (biomedcentral.com)
  • Research into cytokines, and their roles in health and disease, is booming. (barefacedtruth.com)
  • Our work with stem cells as sources of cytokines has driven us to a deeper understanding, and appreciation, for the complexities of cytokines. (barefacedtruth.com)
  • Exploring the key differences between the inflammatory healing associated with older age, and the scarless fetal healing, focusing on several cytokines at the center of this phenomenon. (barefacedtruth.com)
  • In other words, we have found that chronic stress leads to impairment of dermal matrix remodelling which has clear implications with respect to skin condition. (allindianpatents.com)
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • Non-traditional sources of MSCs, including Wharton's jelly and medical waste material, have shown efficacy comparable to that of traditional sources, such as bone marrow and adipose tissue. (biomedcentral.com)
  • Reconstruction of peripheral nerve defects with tissue engineered nerve scaffolds is an exciting field of biomedical research and holds potential for clinical application. (bvsalud.org)
  • and altering cell and tissue characteristics for biomedical research and manufacturing. (nationalacademies.org)
  • Various decellularization methods have been described in literature, and they involve removal of cells and intracellular components using various chemical (such as acids, bases, and detergents), biological (such as enzymes, chelation agents, and toxins), or physical (such as freeze-thaw cycling) agents. (researchsquare.com)
  • The protocols for decellularization are dependent on cell and tissue type and species of origin usually requiring multiple chemical and biological reagents supplied in a series of incubations 6 . (researchsquare.com)
  • Hence, a major threat for the clinical use of dermal substitutes is insufficient vascularization leading to loosening, infection, or partial necrosis of the dermal substitute [ 7 ]. (hindawi.com)
  • In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
  • The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
  • We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
  • Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
  • Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
  • Angiogenesis is an important factor for tissue-engineered skin constructs. (hindawi.com)
  • Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
  • In orthopaedic non‐load bearing devices or dental implants, the preferred materials can be resorbable ceramics chemically similar to the inorganic component of the bone tissue, such as hydroxyapatite 1 , 2 . (nature.com)
  • Tissue and cell type-specific cell-free extracellular matrices generated by various decellularization protocols can be important tools to study cell interactions with their distinct extracellular niche. (researchsquare.com)
  • This protocol efficiently removes cytoskeletal proteins, including actin and beta-tubulin, and DNA from the cultures, which could interfere with the cell-matrix interactions of cells seeded on the decellularized matrices. (researchsquare.com)
  • Thus, it may refer to the "exosome complex ," being a proteic (enzymatic) macromolecular machinery, present in archaea and eukaryotic cells, being involved in RNA degradation. (frontiersin.org)
  • In order to avoid protease-mediated degradation during processing, decellularization methods also need to include protease inhibitors to inactivate intracellular proteases released during the cell lysis 5,6 . (researchsquare.com)
  • In particular, we have found that chronically stressed cells have a reduced ability to express proteins required for matrix degradation and matrix synthesis - processes that are needed to repair and maintain skin. (allindianpatents.com)
  • Type IV collagen forms the basis of basement membranes, which are thin layers of connective tissue that separate and support various cell layers. (intactone.com)
  • Another group engineered tendon constructs by rolling tenocyte-seeded SIS scaffolds into tubular constructs that could be mounted into custom loading frames for static or dynamic loading within an incubator. (researchgate.net)
  • The structure is essential because native ECM creates space where the cells are anchored, communicate, proliferate, differentiate, and die. (nature.com)
  • At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)
  • Mora MV, Ibán MAR, Heredia JD, Laakso RB, Cuéllar R, Arranz MG. Stem cell therapy in the management of shoulder rotator cuff disorders. (wjgnet.com)
  • Rejuvenation therapies aim to reverse or repair age-related cellular changes such as molecular waste, calcification , tissue stiffening , loss of stem cell function , genetic alterations, and impaired energy production . (fightaging.org)
  • Stem Cell Res. (sciendo.com)
  • From gene therapy to cell therapy to cytokine pharming to cellular conditioned media, to stem cell orchestras producing cytokine symphonies. (barefacedtruth.com)
  • Potentiality of embryonic stem cells: an ethical problem even with alternative stem cell sources. (razavihospital.ir)
  • One classical approach to promoting vascularization is to decorate or supplement the scaffolds with proangiogenic factors such as VEGF, bFGF, and PDGF [ 8 ]. (hindawi.com)
  • Tβ4 also increased the mRNA and protein expression of MMP2 and VEGF in RP cells. (spandidos-publications.com)
  • Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
  • Three cell types are closely related in anatomic and functional aspects. (biomedcentral.com)
  • Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. (razavihospital.ir)
  • Ying QL, Stavridis M, Griffiths D, Li M, Smith A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent Ferro F et al. (razavihospital.ir)
  • Nakano T, Kodama H, Honjo T. Generation of lymphohematopoietic cells from embryonic stem cells in culture. (razavihospital.ir)
  • It involves incubation of the cultures in an alkaline detergent solution (20 mM NH 4 OH/0.5% Triton X-100, pH=8.0) to lyse cell membranes followed by digestion with DNase to remove DNA. (researchsquare.com)
  • In a preferred embodiment, the composition comprises a first substance which is capable of inhibiting glucocorticoid-induced chronic stress in a cell involved in skin inflammatory responses and a second substance which is capable of inhibiting glucocorticoid-induced chronic stress in a dermal cell, provided that said first substance and second substance are different. (allindianpatents.com)
  • Specifically, the timely orchestration of pro- and anti-inflammatory responses, based on the activation and inhibition of various cell types, such as tissue-resident cells and different types of immune cells, can significantly impact wound repair [ 7 ]. (biomedcentral.com)
  • 2022). Fibrillin-1 regulates white adipose tissue development, homeostasis, and function. (mcgill.ca)